Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Jump to well contents.

Supplemental Information

Screen Description

Wizard III&IV

Rigaku / Wizard III&IV
Created 2010-05-28.

Export

Wizard III&IV

Barcodes

Wizard III&IV

    Screen Structure

    Wizard III&IV

    Well Count 96
    Number of distinct ingredients 70
    Internal diversity (score mean dist) 0.963

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:52:15.639836 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Wizard III&IV
    Buffer 98
    Cryoprotectant 7
    Organic (non-volatile) 32
    Organic (volatile) 11
    Polymer 74
    Salt 89

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Wizard III&IV Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium hydrogen citrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    520 μl of water (100 v/v)
    A2
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.02 M -
    		pH 4.6
    19 μl of Sodium acetate pH 3.6 (3 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    13 μl of Sodium acetate pH 5.6 (3 M)
    10 μl of Calcium chloride (2 M)
    656 μl of water (100 v/v)
    A3
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium formate 0.2 M -
    		NA
    200 μl of Magnesium formate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A4
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium formate 0.2 M -
    		NA
    20 μl of Ammonium formate (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium chloride 0.2 M -
    		NA
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium formate 0.2 M -
    		NA
    14 μl of Potassium formate (14 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    585 μl of water (100 v/v)
    A7
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 0.2 M -
    		pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of water (100 v/v)
    A8
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium nitrate 0.2 M -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Potassium nitrate (3 M)
    533 μl of water (100 v/v)
    A9
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    		pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    A10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium thiocyanate 0.2 M -
    		NA
    25 μl of Sodium thiocyanate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    A11
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 9
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    A12
    • Polyethylene glycol 8,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 8 v/v -
    		pH 7.5
    80 μl of Ethylene glycol (100 v/v)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    B1
    • Polyethylene glycol 4,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    19 μl of Sodium acetate pH 3.6 (3 M)
    13 μl of Sodium acetate pH 5.6 (3 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    806 μl of water (100 v/v)
    B2
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    B3
    • tri-Sodium citrate 1.6 M -
    		NA
    400 μl of tri-Sodium citrate (4 M)
    600 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Potassium citrate 0.2 M -
    		NA
    133 μl of tri-Potassium citrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    B5
    • Polyethylene glycol 4,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-Propanol 10 v/v -
    		pH 5.5
    188 μl of tri-Sodium citrate pH 6 (0.5 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    11 μl of tri-Sodium citrate pH 3 (0.5 M)
    300 μl of water (100 v/v)
    B6
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 4
    • Lithium chloride 1 M -
    		pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium nitrate 0.2 M -
    		NA
    20 μl of Ammonium nitrate (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B8
    • Polyethylene glycol 6,000 10 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    700 μl of water (100 v/v)
    B9
    • Sodium Potassium phosphate 1.6 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    533 μl of Sodium Potassium phosphate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    366 μl of water (100 v/v)
    B10
    • Ethanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    200 μl of Ethanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    B11
    • Polyethylene glycol 20,000 10 w/v -
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    20 μl of 1,4-Dioxane (100 v/v)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    546 μl of water (100 v/v)
    B12
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    		pH 4.6
    19 μl of Sodium acetate pH 3.6 (3 M)
    13 μl of Sodium acetate pH 5.6 (3 M)
    571 μl of Ammonium sulfate (3.5 M)
    395 μl of water (100 v/v)
    C1
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    		NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    C2
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    		NA
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Glycerol (100 v/v)
    320 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    		pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    500 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    C5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    C6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Ammonium sulfate 0.17 M -
    • Glycerol 15 v/v -
    		NA
    150 μl of Glycerol (100 v/v)
    48 μl of Ammonium sulfate (3.5 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    291 μl of water (100 v/v)
    C7
    • 2-Propanol 14 v/v -
    • Sodium acetate 0.07 M pH 4.6
    • Calcium chloride 0.14 M -
    • Glycerol 30 v/v -
    		pH 4.6
    13 μl of Sodium acetate pH 3.6 (3 M)
    9 μl of Sodium acetate pH 5.6 (3 M)
    140 μl of 2-Propanol (100 v/v)
    70 μl of Calcium chloride (2 M)
    300 μl of Glycerol (100 v/v)
    466 μl of water (100 v/v)
    C8
    • Polyethylene glycol 8,000 16 w/v -
    • Potassium di-hydrogen phosphate 0.04 M -
    • Glycerol 20 v/v -
    		NA
    26 μl of Potassium di-hydrogen phosphate (1.5 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    453 μl of water (100 v/v)
    C9
    • Magnesium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    800 μl of Magnesium sulfate (2 M)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    C10
    • Polyethylene glycol 6,000 10 w/v -
    • BICINE 0.1 M pH 9
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C11
    • Polyethylene glycol 8,000 14.4 w/v -
    • Sodium cacodylate 0.08 M pH 6.5
    • Calcium acetate 0.16 M -
    • Glycerol 20 v/v -
    		pH 6.5
    24 μl of Sodium cacodylate pH 5 (1 M)
    55 μl of Sodium cacodylate pH 7.4 (1 M)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    160 μl of Calcium acetate (1 M)
    272 μl of water (100 v/v)
    C12
    • Jeffamine® M-600 30 v/v -
    • MES 0.1 M pH 6.5
    • Cesium chloride 0.05 M -
    		pH 6.5
    300 μl of Jeffamine® M-600 (100 v/v)
    50 μl of Cesium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    550 μl of water (100 v/v)
    D1
    • Ammonium sulfate 3.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    800 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of water (100 v/v)
    D2
    • Polyethylene glycol 10,000 15 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • 1,4-Dioxane 2 v/v -
    		pH 5.5
    20 μl of 1,4-Dioxane (100 v/v)
    188 μl of tri-Sodium citrate pH 6 (0.5 M)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    11 μl of tri-Sodium citrate pH 3 (0.5 M)
    480 μl of water (100 v/v)
    D3
    • Jeffamine® M-600 20 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    200 μl of Jeffamine® M-600 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of water (100 v/v)
    D4
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • BICINE 0.1 M pH 9
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    800 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 28 v/v -
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    		pH 7.5
    280 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    220 μl of water (100 v/v)
    D7
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    		NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    342 μl of water (100 v/v)
    D8
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium sulfate 0.2 M -
    		pH 8
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Lithium sulfate (2.5 M)
    220 μl of water (100 v/v)
    D9
    • Ammonium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 12 v/v -
    		pH 8.5
    120 μl of Glycerol (100 v/v)
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    351 μl of water (100 v/v)
    D10
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium chloride 200 mM -
    		pH 8.5
    400 μl of Ammonium chloride (500 mM)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    D11
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    57 μl of Ammonium sulfate (3.5 M)
    142 μl of water (100 v/v)
    D12
    • Polyethylene glycol 10,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    E1
    • Glycerol 20 v/v -
    • di-Potassium hydrogen phosphate 40 mM -
    • Polyethylene glycol 8,000 16 w/v -
    		NA
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    20 μl of di-Potassium hydrogen phosphate (2000 mM)
    200 μl of Glycerol (100 v/v)
    460 μl of water (100 v/v)
    E2
    • Ethanol 15 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 100 mM -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    		pH 8
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    150 μl of Ethanol (100 v/v)
    40 μl of Sodium chloride (2500 mM)
    660 μl of water (100 v/v)
    E3
    • Ethanol 40 v/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 5 w/v -
    		pH 4.2
    400 μl of Ethanol (100 v/v)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    400 μl of water (100 v/v)
    E4
    • Ammonium sulfate 200 mM -
    • BIS-TRIS 0.1 M pH 5.5
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium sulfate (1000 mM)
    700 μl of water (100 v/v)
    E5
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 400 2 v/v -
    		pH 5.5
    1 μl of Sodium acetate pH 3.6 (3 M)
    500 μl of Ammonium sulfate (4 M)
    32 μl of Sodium acetate pH 5.6 (3 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    446 μl of water (100 v/v)
    E6
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    		pH 4
    10 μl of tri-Sodium citrate pH 3 (1.6 M)
    51 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    228 μl of Ammonium sulfate (3.5 M)
    708 μl of water (100 v/v)
    E7
    • Lithium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Magnesium sulfate 100 mM -
    • 2-Propanol 5 v/v -
    		pH 4.5
    200 μl of Magnesium sulfate (500 mM)
    50 μl of 2-Propanol (100 v/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    400 μl of Lithium sulfate (5 M)
    316 μl of water (100 v/v)
    E8
    • Lithium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 2 v/v -
    		pH 8.5
    20 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Lithium sulfate (5 M)
    480 μl of water (100 v/v)
    E9
    • Lithium sulfate 2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Magnesium sulfate 100 mM -
    • Polyethylene glycol 400 5 v/v -
    		pH 5.5
    1 μl of Sodium acetate pH 3.6 (3 M)
    200 μl of Magnesium sulfate (500 mM)
    32 μl of Sodium acetate pH 5.6 (3 M)
    50 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium sulfate (5 M)
    316 μl of water (100 v/v)
    E10
    • Polyethylene glycol 200 50 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Magnesium chloride 200 mM -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    500 μl of Polyethylene glycol 200 (100 v/v)
    400 μl of Magnesium chloride (500 mM)
    E11
    • Polyethylene glycol 300 40 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Calcium acetate 200 mM -
    		pH 6.5
    31 μl of Sodium cacodylate pH 5 (1 M)
    68 μl of Sodium cacodylate pH 7.4 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    400 μl of Calcium acetate (500 mM)
    100 μl of water (100 v/v)
    E12
    • Jeffamine® M-600 30 v/v -
    • HEPES 0.1 M pH 7
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    300 μl of water (100 v/v)
    F1
    • di-Sodium succinate 800 mM pH 7
    		pH 7
    800 μl of di-Sodium succinate pH 7 (1000 mM)
    200 μl of water (100 v/v)
    F2
    • Polyethylene glycol 400 40 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 200 mM -
    		pH 8.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium sulfate (500 mM)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of water (100 v/v)
    F3
    • Polyethylene glycol 400 50 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 200 mM -
    		pH 4.5
    500 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    400 μl of Lithium sulfate (500 mM)
    66 μl of water (100 v/v)
    F4
    • Polyethylene glycol monomethyl ether 550 15 v/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    600 μl of water (100 v/v)
    F5
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 5.5
    		pH 5.5
    100 μl of SPG buffer pH 5.5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    F6
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 8.5
    		pH 8.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of SPG buffer pH 8.5 (1 M)
    400 μl of water (100 v/v)
    F7
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 6.5
    		pH 6.5
    100 μl of MMT buffer pH 6.5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    F8
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 9
    		pH 9
    100 μl of MMT buffer pH 9 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    F9
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 5
    		pH 5
    100 μl of MIB buffer pH 5 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    F10
    • Polyethylene glycol 1,500 25 w/v -
    • PCB buffer 0.1 M pH 7
    		pH 7
    100 μl of PCB buffer pH 7 (1 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    F11
    • Polyethylene glycol 1,500 12 w/v -
    • Sodium acetate 0.1 M pH 5.5
    • Sodium chloride 2.5 M -
    • 2-methyl-2,4-pentanediol BCC 1.5 v/v -
    		pH 5.5
    1 μl of Sodium acetate pH 3.6 (3 M)
    15 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    32 μl of Sodium acetate pH 5.6 (3 M)
    240 μl of Polyethylene glycol 1,500 (50 w/v)
    500 μl of Sodium chloride (5 M)
    211 μl of water (100 v/v)
    F12
    • di-Sodium malonate 2.4 M -
    		NA
    800 μl of di-Sodium malonate (3 M)
    200 μl of water (100 v/v)
    G1
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium bromide 150 mM -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of Potassium bromide (500 mM)
    100 μl of water (100 v/v)
    G2
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Sodium acetate 0.1 M pH 5.5
    • Ammonium sulfate 200 mM -
    		pH 5.5
    1 μl of Sodium acetate pH 3.6 (3 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    32 μl of Sodium acetate pH 5.6 (3 M)
    200 μl of Ammonium sulfate (1000 mM)
    566 μl of water (100 v/v)
    G3
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Trimethylamine N-oxide 200 mM -
    		pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Trimethylamine N-oxide (500 mM)
    100 μl of water (100 v/v)
    G4
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium fluoride 200 mM -
    		pH 6.5
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of Sodium fluoride (500 mM)
    100 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.3 M pH 4
    		pH 4
    300 μl of tri-Sodium citrate pH 4 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    G6
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • di-Sodium malonate 200 mM -
    		pH 8.5
    66 μl of di-Sodium malonate (3000 mM)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    433 μl of water (100 v/v)
    G7
    • Poly(acrylic acid sodium salt) 5,100 20 w/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 20 mM -
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    40 μl of Magnesium chloride (500 mM)
    400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    460 μl of water (100 v/v)
    G8
    • di-Sodium DL-malate 2.1e+03 mM pH 7
    		pH 7
    840 μl of di-Sodium DL-malate pH 7 (2500 mM)
    160 μl of water (100 v/v)
    G9
    • di-Potassium hydrogen phosphate 800 mM -
    • HEPES 0.1 M pH 7.5
    • Sodium di-hydrogen phosphate 800 mM -
    		pH 7.5
    400 μl of di-Potassium hydrogen phosphate (2000 mM)
    400 μl of Sodium di-hydrogen phosphate (2000 mM)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    G10
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Ammonium chloride 200 mM -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of Ammonium chloride (500 mM)
    G11
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 200 mM -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    50 μl of Sodium chloride (4000 mM)
    450 μl of water (100 v/v)
    G12
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium chloride 200 mM -
    		pH 8
    400 μl of Lithium chloride (500 mM)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of water (100 v/v)
    H1
    • Polyvinylpyrrolidone K15 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Cobalt (II) chloride 100 mM -
    		pH 8.5
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of Cobalt (II) chloride (300 mM)
    166 μl of water (100 v/v)
    H2
    • Ethylene glycol 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 200 mM -
    		pH 8.5
    500 μl of Ethylene glycol (100 v/v)
    400 μl of Magnesium chloride (500 mM)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    H3
    • Polyethylene glycol 8,000 20 w/v -
    • Imidazole 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 3 v/v -
    		pH 6.5
    30 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    470 μl of water (100 v/v)
    H4
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 100 mM -
    • Polyethylene glycol 400 20 v/v -
    		pH 8.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 400 (100 v/v)
    200 μl of Magnesium chloride (500 mM)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of water (100 v/v)
    H5
    • Polyethylene glycol 8,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 200 mM -
    • 2-Propanol 10 v/v -
    		pH 7.5
    100 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Ammonium sulfate (1000 mM)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of water (100 v/v)
    H6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,500 25 w/v -
    		pH 4.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    166 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Imidazole 0.1 M pH 6.5
    • Ammonium sulfate 200 mM -
    • Polyethylene glycol 3,350 10 w/v -
    		pH 6.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of Ammonium sulfate (1000 mM)
    100 μl of Imidazole pH 6.5 (1 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.5 M -
    • Polyethylene glycol 8,000 8 w/v -
    		pH 8.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    340 μl of water (100 v/v)
    H9
    • 2-Propanol 40 v/v -
    • Imidazole 0.1 M pH 6.5
    • Polyethylene glycol 8,000 15 w/v -
    		pH 6.5
    400 μl of 2-Propanol (100 v/v)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    200 μl of water (100 v/v)
    H10
    • 2-Propanol 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 30 w/v -
    		pH 8.5
    300 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    H11
    • Polyethylene glycol 10,000 17 w/v -
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium acetate 100 mM -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    333 μl of Ammonium acetate (300 mM)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    226 μl of water (100 v/v)
    H12
    • Polyethylene glycol 20,000 15 w/v -
    • HEPES 0.1 M pH 7
    		pH 7
    500 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of HEPES pH 7 (1 M)
    400 μl of water (100 v/v)