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Supplemental Information

Screen Description

Pi PEG

Pi screens / Pi PEG
Created 2011-06-15.

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Pi PEG

    Screen Structure

    Pi PEG

    Well Count 96
    Number of distinct ingredients 18
    Internal diversity (score mean dist) 0.920

    Screen Composition

    2025-06-13T21:23:02.976794 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Pi PEG
    Buffer 96
    Organic (non-volatile) 28
    Polymer 168
    Salt 16

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Pi PEG Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 200 22.5 v/v -
    • Sodium acetate 0.05 M pH 4.8
    pH 4.8
    225 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    725 μl of water (100 v/v)
    A2
    • Polyethylene glycol 300 20 v/v -
    • Sodium acetate 0.05 M pH 5.2
    pH 5.2
    200 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    750 μl of water (100 v/v)
    A3
    • Polyethylene glycol monomethyl ether 350 20 v/v -
    • MES 0.05 M pH 5.6
    pH 5.6
    50 μl of MES pH 5.6 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    750 μl of water (100 v/v)
    A4
    • Polyethylene glycol 400 20 v/v -
    • MES 0.05 M pH 6
    pH 6
    200 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of MES pH 6 (1 M)
    750 μl of water (100 v/v)
    A5
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • ADA 0.05 M pH 6.4
    pH 6.4
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of ADA pH 6.4 (1 M)
    750 μl of water (100 v/v)
    A6
    • Polyethylene glycol 600 20 v/v -
    • ADA 0.05 M pH 6.8
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    400 μl of Polyethylene glycol 600 (50 v/v)
    550 μl of water (100 v/v)
    A7
    • Polyethylene glycol 1,000 17.5 w/v -
    • HEPES 0.05 M pH 7.1
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    350 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    A8
    • Polyethylene glycol 1,500 17.5 w/v -
    • HEPES 0.05 M pH 7.3
    pH 7.3
    350 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of HEPES pH 7.3 (1 M)
    600 μl of water (100 v/v)
    A9
    • Polyethylene glycol 2,000 15 w/v -
    • tris chloride BCC 0.05 M pH 7.6
    pH 7.6
    50 μl of tris chloride BCC pH 7.6 (1 M)
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    650 μl of water (100 v/v)
    A10
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • tris chloride BCC 0.05 M pH 8
    pH 8
    50 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    650 μl of water (100 v/v)
    A11
    • Polyethylene glycol 3,000 15 w/v -
    • BICINE 0.05 M pH 8.4
    pH 8.4
    50 μl of BICINE pH 8.4 (1 M)
    300 μl of Polyethylene glycol 3,000 (50 w/v)
    650 μl of water (100 v/v)
    A12
    • Polyethylene glycol 4,000 15 w/v -
    • BICINE 0.05 M pH 8.8
    pH 8.8
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of BICINE pH 8.8 (1 M)
    650 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,500 15 w/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol 4,000 3.6 w/v -
    pH 4.8
    72 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    578 μl of water (100 v/v)
    B2
    • Polyethylene glycol 2,000 12.9 w/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol 200 6.4 v/v -
    pH 5.2
    64 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    258 μl of Polyethylene glycol 2,000 (50 w/v)
    628 μl of water (100 v/v)
    B3
    • Polyethylene glycol monomethyl ether 2,000 12.9 w/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol 300 5.7 v/v -
    pH 5.6
    57 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of MES pH 5.6 (1 M)
    258 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    635 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,000 12.9 w/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol monomethyl ether 350 5.7 v/v -
    pH 6
    258 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of MES pH 6 (1 M)
    57 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    635 μl of water (100 v/v)
    B5
    • Polyethylene glycol 4,000 12.9 w/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol 400 5.7 v/v -
    pH 6.4
    258 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of ADA pH 6.4 (1 M)
    635 μl of water (100 v/v)
    B6
    • Polyethylene glycol 200 19.3 v/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol monomethyl ether 550 5.7 v/v -
    pH 6.8
    57 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of ADA pH 6.8 (1 M)
    193 μl of Polyethylene glycol 200 (100 v/v)
    700 μl of water (100 v/v)
    B7
    • Polyethylene glycol 300 17.1 v/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol 600 5.7 v/v -
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    171 μl of Polyethylene glycol 300 (100 v/v)
    114 μl of Polyethylene glycol 600 (50 v/v)
    665 μl of water (100 v/v)
    B8
    • Polyethylene glycol monomethyl ether 350 17.1 v/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol 1,000 5 w/v -
    pH 7.3
    50 μl of HEPES pH 7.3 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    171 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    679 μl of water (100 v/v)
    B9
    • Polyethylene glycol 400 17.1 v/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol 1,500 5 w/v -
    pH 7.6
    100 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    171 μl of Polyethylene glycol 400 (100 v/v)
    679 μl of water (100 v/v)
    B10
    • Polyethylene glycol monomethyl ether 550 17.1 v/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 2,000 4.3 w/v -
    pH 8
    171 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    86 μl of Polyethylene glycol 2,000 (50 w/v)
    693 μl of water (100 v/v)
    B11
    • Polyethylene glycol 600 17.1 v/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol monomethyl ether 2,000 4.3 w/v -
    pH 8.4
    50 μl of BICINE pH 8.4 (1 M)
    342 μl of Polyethylene glycol 600 (50 v/v)
    86 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    522 μl of water (100 v/v)
    B12
    • Polyethylene glycol 1,000 15 w/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol 3,000 4.3 w/v -
    pH 8.8
    86 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of BICINE pH 8.8 (1 M)
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    564 μl of water (100 v/v)
    C1
    • Polyethylene glycol monomethyl ether 350 14.3 v/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol 300 11.4 v/v -
    pH 4.8
    114 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    143 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    693 μl of water (100 v/v)
    C2
    • Polyethylene glycol 400 14.3 v/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol monomethyl ether 350 11.4 v/v -
    pH 5.2
    143 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    114 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    693 μl of water (100 v/v)
    C3
    • Polyethylene glycol monomethyl ether 550 14.3 v/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol 400 11.4 v/v -
    pH 5.6
    143 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of MES pH 5.6 (1 M)
    114 μl of Polyethylene glycol 400 (100 v/v)
    693 μl of water (100 v/v)
    C4
    • Polyethylene glycol 600 14.3 v/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol monomethyl ether 550 11.4 v/v -
    pH 6
    114 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    286 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of MES pH 6 (1 M)
    550 μl of water (100 v/v)
    C5
    • Polyethylene glycol 1,000 12.5 w/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol 600 11.4 v/v -
    pH 6.4
    228 μl of Polyethylene glycol 600 (50 v/v)
    250 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of ADA pH 6.4 (1 M)
    472 μl of water (100 v/v)
    C6
    • Polyethylene glycol 1,500 12.5 w/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 1,000 10 w/v -
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    250 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    500 μl of water (100 v/v)
    C7
    • Polyethylene glycol 2,000 10.7 w/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol 1,500 10 w/v -
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    214 μl of Polyethylene glycol 2,000 (50 w/v)
    536 μl of water (100 v/v)
    C8
    • Polyethylene glycol monomethyl ether 2,000 10.7 w/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol 2,000 8.6 w/v -
    pH 7.3
    50 μl of HEPES pH 7.3 (1 M)
    214 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    172 μl of Polyethylene glycol 2,000 (50 w/v)
    564 μl of water (100 v/v)
    C9
    • Polyethylene glycol 3,000 10.7 w/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol monomethyl ether 2,000 8.6 w/v -
    pH 7.6
    214 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    172 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    564 μl of water (100 v/v)
    C10
    • Polyethylene glycol 4,000 10.7 w/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 3,000 8.6 w/v -
    pH 8
    214 μl of Polyethylene glycol 4,000 (50 w/v)
    172 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    564 μl of water (100 v/v)
    C11
    • Polyethylene glycol 200 16.1 v/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol 4,000 7.1 w/v -
    pH 8.4
    142 μl of Polyethylene glycol 4,000 (50 w/v)
    161 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of BICINE pH 8.4 (1 M)
    647 μl of water (100 v/v)
    C12
    • Polyethylene glycol 300 14.3 v/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol 200 12.9 v/v -
    pH 8.8
    129 μl of Polyethylene glycol 200 (100 v/v)
    143 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of BICINE pH 8.8 (1 M)
    678 μl of water (100 v/v)
    D1
    • Polyethylene glycol monomethyl ether 2,000 8.6 w/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol 400 17.1 v/v -
    pH 4.8
    50 μl of Sodium acetate pH 4.8 (1 M)
    171 μl of Polyethylene glycol 400 (100 v/v)
    172 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    607 μl of water (100 v/v)
    D2
    • Polyethylene glycol 3,000 8.6 w/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol monomethyl ether 550 17.1 v/v -
    pH 5.2
    171 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    172 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    607 μl of water (100 v/v)
    D3
    • Polyethylene glycol 4,000 8.6 w/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol 600 17.1 v/v -
    pH 5.6
    172 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of MES pH 5.6 (1 M)
    342 μl of Polyethylene glycol 600 (50 v/v)
    436 μl of water (100 v/v)
    D4
    • Polyethylene glycol 200 12.9 v/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol 1,000 15 w/v -
    pH 6
    129 μl of Polyethylene glycol 200 (100 v/v)
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of MES pH 6 (1 M)
    521 μl of water (100 v/v)
    D5
    • Polyethylene glycol 300 11.4 v/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol 1,500 15 w/v -
    pH 6.4
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    114 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of ADA pH 6.4 (1 M)
    536 μl of water (100 v/v)
    D6
    • Polyethylene glycol monomethyl ether 350 11.4 v/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 2,000 12.9 w/v -
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    114 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    258 μl of Polyethylene glycol 2,000 (50 w/v)
    578 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 11.4 v/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol monomethyl ether 2,000 12.9 w/v -
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    114 μl of Polyethylene glycol 400 (100 v/v)
    258 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    578 μl of water (100 v/v)
    D8
    • Polyethylene glycol monomethyl ether 550 11.4 v/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol 3,000 12.9 w/v -
    pH 7.3
    114 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    258 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of HEPES pH 7.3 (1 M)
    578 μl of water (100 v/v)
    D9
    • Polyethylene glycol 600 11.4 v/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol 4,000 10.7 w/v -
    pH 7.6
    214 μl of Polyethylene glycol 4,000 (50 w/v)
    228 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    508 μl of water (100 v/v)
    D10
    • Polyethylene glycol 1,000 10 w/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 200 19.3 v/v -
    pH 8
    193 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    557 μl of water (100 v/v)
    D11
    • Polyethylene glycol 1,500 10 w/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol 300 17.1 v/v -
    pH 8.4
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    171 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of BICINE pH 8.4 (1 M)
    579 μl of water (100 v/v)
    D12
    • Polyethylene glycol 2,000 8.6 w/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol monomethyl ether 350 17.1 v/v -
    pH 8.8
    50 μl of BICINE pH 8.8 (1 M)
    171 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    172 μl of Polyethylene glycol 2,000 (50 w/v)
    607 μl of water (100 v/v)
    E1
    • Polyethylene glycol monomethyl ether 550 8.6 v/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol 600 22.9 v/v -
    pH 4.8
    86 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    458 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    406 μl of water (100 v/v)
    E2
    • Polyethylene glycol 600 8.6 v/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol 1,000 20 w/v -
    pH 5.2
    172 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    378 μl of water (100 v/v)
    E3
    • Polyethylene glycol 1,000 7.5 w/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol 1,500 20 w/v -
    pH 5.6
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of MES pH 5.6 (1 M)
    150 μl of Polyethylene glycol 1,000 (50 w/v)
    400 μl of water (100 v/v)
    E4
    • Polyethylene glycol 1,500 7.5 w/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol 2,000 17.1 w/v -
    pH 6
    150 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of MES pH 6 (1 M)
    342 μl of Polyethylene glycol 2,000 (50 w/v)
    458 μl of water (100 v/v)
    E5
    • Polyethylene glycol 2,000 6.4 w/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol monomethyl ether 2,000 17.1 w/v -
    pH 6.4
    342 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of ADA pH 6.4 (1 M)
    128 μl of Polyethylene glycol 2,000 (50 w/v)
    480 μl of water (100 v/v)
    E6
    • Polyethylene glycol monomethyl ether 2,000 6.4 w/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 3,000 17.1 w/v -
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    342 μl of Polyethylene glycol 3,000 (50 w/v)
    128 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    480 μl of water (100 v/v)
    E7
    • Polyethylene glycol 3,000 6.4 w/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol 4,000 14.3 w/v -
    pH 7.1
    286 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of HEPES pH 7.1 (1 M)
    128 μl of Polyethylene glycol 3,000 (50 w/v)
    536 μl of water (100 v/v)
    E8
    • Polyethylene glycol 4,000 6.4 w/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol 200 25.7 v/v -
    pH 7.3
    128 μl of Polyethylene glycol 4,000 (50 w/v)
    257 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of HEPES pH 7.3 (1 M)
    565 μl of water (100 v/v)
    E9
    • Polyethylene glycol 200 9.6 v/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol 300 22.9 v/v -
    pH 7.6
    96 μl of Polyethylene glycol 200 (100 v/v)
    229 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    625 μl of water (100 v/v)
    E10
    • Polyethylene glycol 300 8.6 v/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol monomethyl ether 350 22.9 v/v -
    pH 8
    86 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    229 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    635 μl of water (100 v/v)
    E11
    • Polyethylene glycol monomethyl ether 350 8.6 v/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol 400 22.9 v/v -
    pH 8.4
    50 μl of BICINE pH 8.4 (1 M)
    229 μl of Polyethylene glycol 400 (100 v/v)
    86 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    635 μl of water (100 v/v)
    E12
    • Polyethylene glycol 400 8.6 v/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol monomethyl ether 550 22.9 v/v -
    pH 8.8
    229 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of BICINE pH 8.8 (1 M)
    86 μl of Polyethylene glycol 400 (100 v/v)
    635 μl of water (100 v/v)
    F1
    • Polyethylene glycol 4,000 4.3 w/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol 1,500 25 w/v -
    pH 4.8
    86 μl of Polyethylene glycol 4,000 (50 w/v)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    364 μl of water (100 v/v)
    F2
    • Polyethylene glycol 200 6.4 v/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol 2,000 21.4 w/v -
    pH 5.2
    64 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    428 μl of Polyethylene glycol 2,000 (50 w/v)
    458 μl of water (100 v/v)
    F3
    • Polyethylene glycol 300 5.7 v/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol monomethyl ether 2,000 21.4 w/v -
    pH 5.6
    57 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of MES pH 5.6 (1 M)
    428 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    465 μl of water (100 v/v)
    F4
    • Polyethylene glycol monomethyl ether 350 5.7 v/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol 3,000 21.4 w/v -
    pH 6
    428 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of MES pH 6 (1 M)
    57 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    465 μl of water (100 v/v)
    F5
    • Polyethylene glycol 400 5.7 v/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol 4,000 17.9 w/v -
    pH 6.4
    358 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of ADA pH 6.4 (1 M)
    535 μl of water (100 v/v)
    F6
    • Polyethylene glycol monomethyl ether 550 5.7 v/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 200 32.1 v/v -
    pH 6.8
    57 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of ADA pH 6.8 (1 M)
    321 μl of Polyethylene glycol 200 (100 v/v)
    572 μl of water (100 v/v)
    F7
    • Polyethylene glycol 600 5.7 v/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol 300 28.6 v/v -
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    286 μl of Polyethylene glycol 300 (100 v/v)
    114 μl of Polyethylene glycol 600 (50 v/v)
    550 μl of water (100 v/v)
    F8
    • Polyethylene glycol 1,000 5 w/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol monomethyl ether 350 28.6 v/v -
    pH 7.3
    50 μl of HEPES pH 7.3 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    286 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    564 μl of water (100 v/v)
    F9
    • Polyethylene glycol 1,500 5 w/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol 400 28.6 v/v -
    pH 7.6
    100 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    286 μl of Polyethylene glycol 400 (100 v/v)
    564 μl of water (100 v/v)
    F10
    • Polyethylene glycol 2,000 4.3 w/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol monomethyl ether 550 28.6 v/v -
    pH 8
    286 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    86 μl of Polyethylene glycol 2,000 (50 w/v)
    578 μl of water (100 v/v)
    F11
    • Polyethylene glycol monomethyl ether 2,000 4.3 w/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol 600 28.6 v/v -
    pH 8.4
    50 μl of BICINE pH 8.4 (1 M)
    86 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    286 μl of Polyethylene glycol 600 (100 v/v)
    578 μl of water (100 v/v)
    F12
    • Polyethylene glycol 3,000 4.3 w/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol 1,000 25 w/v -
    pH 8.8
    86 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of BICINE pH 8.8 (1 M)
    500 μl of Polyethylene glycol 1,000 (50 w/v)
    364 μl of water (100 v/v)
    G1
    • Polyethylene glycol 1,000 2.5 w/v -
    • Sodium acetate 0.05 M pH 4.8
    • Polyethylene glycol monomethyl ether 2,000 25.7 w/v -
    pH 4.8
    50 μl of Sodium acetate pH 4.8 (1 M)
    514 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of Polyethylene glycol 1,000 (50 w/v)
    386 μl of water (100 v/v)
    G2
    • Polyethylene glycol 1,500 2.5 w/v -
    • Sodium acetate 0.05 M pH 5.2
    • Polyethylene glycol 3,000 25.7 w/v -
    pH 5.2
    50 μl of Polyethylene glycol 1,500 (50 w/v)
    514 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    386 μl of water (100 v/v)
    G3
    • Polyethylene glycol 2,000 2.1 w/v -
    • MES 0.05 M pH 5.6
    • Polyethylene glycol 4,000 21.4 w/v -
    pH 5.6
    428 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of MES pH 5.6 (1 M)
    42 μl of Polyethylene glycol 2,000 (50 w/v)
    480 μl of water (100 v/v)
    G4
    • Polyethylene glycol monomethyl ether 2,000 2.1 w/v -
    • MES 0.05 M pH 6
    • Polyethylene glycol 200 38.6 v/v -
    pH 6
    386 μl of Polyethylene glycol 200 (100 v/v)
    42 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of MES pH 6 (1 M)
    522 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,000 2.1 w/v -
    • ADA 0.05 M pH 6.4
    • Polyethylene glycol 300 34.3 v/v -
    pH 6.4
    343 μl of Polyethylene glycol 300 (100 v/v)
    42 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of ADA pH 6.4 (1 M)
    565 μl of water (100 v/v)
    G6
    • Polyethylene glycol 4,000 2.1 w/v -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol monomethyl ether 350 34.3 v/v -
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    42 μl of Polyethylene glycol 4,000 (50 w/v)
    343 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    565 μl of water (100 v/v)
    G7
    • Polyethylene glycol 200 3.2 v/v -
    • HEPES 0.05 M pH 7.1
    • Polyethylene glycol 400 34.3 v/v -
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    32 μl of Polyethylene glycol 200 (100 v/v)
    343 μl of Polyethylene glycol 400 (100 v/v)
    575 μl of water (100 v/v)
    G8
    • Polyethylene glycol 300 2.9 v/v -
    • HEPES 0.05 M pH 7.3
    • Polyethylene glycol monomethyl ether 550 34.3 v/v -
    pH 7.3
    343 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    29 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of HEPES pH 7.3 (1 M)
    578 μl of water (100 v/v)
    G9
    • Polyethylene glycol monomethyl ether 350 2.9 v/v -
    • tris chloride BCC 0.05 M pH 7.6
    • Polyethylene glycol 600 34.3 v/v -
    pH 7.6
    686 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    29 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    235 μl of water (100 v/v)
    G10
    • Polyethylene glycol 400 2.9 v/v -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 1,000 30 w/v -
    pH 8
    29 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    321 μl of water (100 v/v)
    G11
    • Polyethylene glycol monomethyl ether 550 2.9 v/v -
    • BICINE 0.05 M pH 8.4
    • Polyethylene glycol 1,500 30 w/v -
    pH 8.4
    29 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of BICINE pH 8.4 (1 M)
    321 μl of water (100 v/v)
    G12
    • Polyethylene glycol 600 2.9 v/v -
    • BICINE 0.05 M pH 8.8
    • Polyethylene glycol 2,000 25.7 w/v -
    pH 8.8
    58 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of BICINE pH 8.8 (1 M)
    514 μl of Polyethylene glycol 2,000 (50 w/v)
    378 μl of water (100 v/v)
    H1
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium acetate 0.05 M pH 4.8
    pH 4.8
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Sodium acetate pH 4.8 (1 M)
    450 μl of water (100 v/v)
    H2
    • Polyethylene glycol 200 45 v/v -
    • Sodium acetate 0.05 M pH 5.2
    pH 5.2
    450 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Sodium acetate pH 5.2 (1 M)
    500 μl of water (100 v/v)
    H3
    • Polyethylene glycol 300 40 v/v -
    • MES 0.05 M pH 5.6
    pH 5.6
    400 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of MES pH 5.6 (1 M)
    550 μl of water (100 v/v)
    H4
    • Polyethylene glycol monomethyl ether 350 40 v/v -
    • MES 0.05 M pH 6
    pH 6
    50 μl of MES pH 6 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    550 μl of water (100 v/v)
    H5
    • Polyethylene glycol 400 40 v/v -
    • ADA 0.05 M pH 6.4
    pH 6.4
    400 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of ADA pH 6.4 (1 M)
    550 μl of water (100 v/v)
    H6
    • Polyethylene glycol monomethyl ether 550 40 v/v -
    • ADA 0.05 M pH 6.8
    pH 6.8
    400 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of ADA pH 6.8 (1 M)
    550 μl of water (100 v/v)
    H7
    • Polyethylene glycol 600 40 v/v -
    • HEPES 0.05 M pH 7.1
    pH 7.1
    50 μl of HEPES pH 7.1 (1 M)
    800 μl of Polyethylene glycol 600 (50 v/v)
    150 μl of water (100 v/v)
    H8
    • Polyethylene glycol 1,000 35 w/v -
    • HEPES 0.05 M pH 7.3
    pH 7.3
    50 μl of HEPES pH 7.3 (1 M)
    700 μl of Polyethylene glycol 1,000 (50 w/v)
    250 μl of water (100 v/v)
    H9
    • Polyethylene glycol 1,500 35 w/v -
    • tris chloride BCC 0.05 M pH 7.6
    pH 7.6
    700 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of tris chloride BCC pH 7.6 (1 M)
    250 μl of water (100 v/v)
    H10
    • Polyethylene glycol 2,000 30 w/v -
    • tris chloride BCC 0.05 M pH 8
    pH 8
    50 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Polyethylene glycol 2,000 (50 w/v)
    350 μl of water (100 v/v)
    H11
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • BICINE 0.05 M pH 8.4
    pH 8.4
    50 μl of BICINE pH 8.4 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    350 μl of water (100 v/v)
    H12
    • Polyethylene glycol 3,000 30 w/v -
    • BICINE 0.05 M pH 8.8
    pH 8.8
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of BICINE pH 8.8 (1 M)
    350 μl of water (100 v/v)