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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
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Pi PEG
Barcodes
Pi PEG
No barcodes found.
Screen Structure
Pi PEG
Well Count | 96 |
---|---|
Number of distinct ingredients | 18 |
Internal diversity (score mean dist) | 0.920 |
Screen Composition
ingredient type | Pi PEG |
---|---|
Buffer | 96 |
Organic (non-volatile) | 28 |
Polymer | 168 |
Salt | 16 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.8 | 225.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 725.0 μl of water (100 v/v) |
A2 |
| pH 5.2 | 200.0 μl of Polyethylene glycol 300 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 750.0 μl of water (100 v/v) |
A3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 750.0 μl of water (100 v/v) |
A4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 750.0 μl of water (100 v/v) |
A5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 750.0 μl of water (100 v/v) |
A6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 400.0 μl of Polyethylene glycol 600 (50 v/v) 550.0 μl of water (100 v/v) |
A7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 350.0 μl of Polyethylene glycol 1,000 (50 w/v) 600.0 μl of water (100 v/v) |
A8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 350.0 μl of Polyethylene glycol 1,500 (50 w/v) 600.0 μl of water (100 v/v) |
A9 |
| pH 7.6 | 300.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 650.0 μl of water (100 v/v) |
A10 |
| pH 8 | 300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 650.0 μl of water (100 v/v) |
A11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 300.0 μl of Polyethylene glycol 3,000 (50 w/v) 650.0 μl of water (100 v/v) |
A12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v) |
B1 |
| pH 4.8 | 300.0 μl of Polyethylene glycol 1,500 (50 w/v) 72.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 578.0 μl of water (100 v/v) |
B2 |
| pH 5.2 | 258.0 μl of Polyethylene glycol 2,000 (50 w/v) 64.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 628.0 μl of water (100 v/v) |
B3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 57.0 μl of Polyethylene glycol 300 (100 v/v) 258.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 635.0 μl of water (100 v/v) |
B4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 258.0 μl of Polyethylene glycol 3,000 (50 w/v) 57.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 635.0 μl of water (100 v/v) |
B5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 258.0 μl of Polyethylene glycol 4,000 (50 w/v) 57.0 μl of Polyethylene glycol 400 (100 v/v) 635.0 μl of water (100 v/v) |
B6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 193.0 μl of Polyethylene glycol 200 (100 v/v) 57.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 700.0 μl of water (100 v/v) |
B7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 171.0 μl of Polyethylene glycol 300 (100 v/v) 114.0 μl of Polyethylene glycol 600 (50 v/v) 665.0 μl of water (100 v/v) |
B8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 171.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 679.0 μl of water (100 v/v) |
B9 |
| pH 7.6 | 100.0 μl of Polyethylene glycol 1,500 (50 w/v) 171.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 679.0 μl of water (100 v/v) |
B10 |
| pH 8 | 86.0 μl of Polyethylene glycol 2,000 (50 w/v) 171.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 693.0 μl of water (100 v/v) |
B11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 342.0 μl of Polyethylene glycol 600 (50 v/v) 86.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 522.0 μl of water (100 v/v) |
B12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 300.0 μl of Polyethylene glycol 1,000 (50 w/v) 86.0 μl of Polyethylene glycol 3,000 (50 w/v) 564.0 μl of water (100 v/v) |
C1 |
| pH 4.8 | 114.0 μl of Polyethylene glycol 300 (100 v/v) 143.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 693.0 μl of water (100 v/v) |
C2 |
| pH 5.2 | 143.0 μl of Polyethylene glycol 400 (100 v/v) 114.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 693.0 μl of water (100 v/v) |
C3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 114.0 μl of Polyethylene glycol 400 (100 v/v) 143.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 693.0 μl of water (100 v/v) |
C4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 286.0 μl of Polyethylene glycol 600 (50 v/v) 114.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 550.0 μl of water (100 v/v) |
C5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 250.0 μl of Polyethylene glycol 1,000 (50 w/v) 228.0 μl of Polyethylene glycol 600 (50 v/v) 472.0 μl of water (100 v/v) |
C6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 250.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
C7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 200.0 μl of Polyethylene glycol 1,500 (50 w/v) 214.0 μl of Polyethylene glycol 2,000 (50 w/v) 536.0 μl of water (100 v/v) |
C8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 172.0 μl of Polyethylene glycol 2,000 (50 w/v) 214.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 564.0 μl of water (100 v/v) |
C9 |
| pH 7.6 | 214.0 μl of Polyethylene glycol 3,000 (50 w/v) 172.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 564.0 μl of water (100 v/v) |
C10 |
| pH 8 | 172.0 μl of Polyethylene glycol 3,000 (50 w/v) 214.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 564.0 μl of water (100 v/v) |
C11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 161.0 μl of Polyethylene glycol 200 (100 v/v) 142.0 μl of Polyethylene glycol 4,000 (50 w/v) 647.0 μl of water (100 v/v) |
C12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 129.0 μl of Polyethylene glycol 200 (100 v/v) 143.0 μl of Polyethylene glycol 300 (100 v/v) 678.0 μl of water (100 v/v) |
D1 |
| pH 4.8 | 171.0 μl of Polyethylene glycol 400 (100 v/v) 172.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 607.0 μl of water (100 v/v) |
D2 |
| pH 5.2 | 172.0 μl of Polyethylene glycol 3,000 (50 w/v) 171.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 607.0 μl of water (100 v/v) |
D3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 172.0 μl of Polyethylene glycol 4,000 (50 w/v) 342.0 μl of Polyethylene glycol 600 (50 v/v) 436.0 μl of water (100 v/v) |
D4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 300.0 μl of Polyethylene glycol 1,000 (50 w/v) 129.0 μl of Polyethylene glycol 200 (100 v/v) 521.0 μl of water (100 v/v) |
D5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 300.0 μl of Polyethylene glycol 1,500 (50 w/v) 114.0 μl of Polyethylene glycol 300 (100 v/v) 536.0 μl of water (100 v/v) |
D6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 258.0 μl of Polyethylene glycol 2,000 (50 w/v) 114.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 578.0 μl of water (100 v/v) |
D7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 114.0 μl of Polyethylene glycol 400 (100 v/v) 258.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 578.0 μl of water (100 v/v) |
D8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 258.0 μl of Polyethylene glycol 3,000 (50 w/v) 114.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 578.0 μl of water (100 v/v) |
D9 |
| pH 7.6 | 214.0 μl of Polyethylene glycol 4,000 (50 w/v) 228.0 μl of Polyethylene glycol 600 (50 v/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 508.0 μl of water (100 v/v) |
D10 |
| pH 8 | 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 193.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 557.0 μl of water (100 v/v) |
D11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 200.0 μl of Polyethylene glycol 1,500 (50 w/v) 171.0 μl of Polyethylene glycol 300 (100 v/v) 579.0 μl of water (100 v/v) |
D12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 172.0 μl of Polyethylene glycol 2,000 (50 w/v) 171.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 607.0 μl of water (100 v/v) |
E1 |
| pH 4.8 | 458.0 μl of Polyethylene glycol 600 (50 v/v) 86.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 406.0 μl of water (100 v/v) |
E2 |
| pH 5.2 | 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 172.0 μl of Polyethylene glycol 600 (50 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 378.0 μl of water (100 v/v) |
E3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 150.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
E4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 150.0 μl of Polyethylene glycol 1,500 (50 w/v) 342.0 μl of Polyethylene glycol 2,000 (50 w/v) 458.0 μl of water (100 v/v) |
E5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 128.0 μl of Polyethylene glycol 2,000 (50 w/v) 342.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 480.0 μl of water (100 v/v) |
E6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 342.0 μl of Polyethylene glycol 3,000 (50 w/v) 128.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 480.0 μl of water (100 v/v) |
E7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 128.0 μl of Polyethylene glycol 3,000 (50 w/v) 286.0 μl of Polyethylene glycol 4,000 (50 w/v) 536.0 μl of water (100 v/v) |
E8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 257.0 μl of Polyethylene glycol 200 (100 v/v) 128.0 μl of Polyethylene glycol 4,000 (50 w/v) 565.0 μl of water (100 v/v) |
E9 |
| pH 7.6 | 96.0 μl of Polyethylene glycol 200 (100 v/v) 229.0 μl of Polyethylene glycol 300 (100 v/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 625.0 μl of water (100 v/v) |
E10 |
| pH 8 | 86.0 μl of Polyethylene glycol 300 (100 v/v) 229.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 635.0 μl of water (100 v/v) |
E11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 229.0 μl of Polyethylene glycol 400 (100 v/v) 86.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 635.0 μl of water (100 v/v) |
E12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 86.0 μl of Polyethylene glycol 400 (100 v/v) 229.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 635.0 μl of water (100 v/v) |
F1 |
| pH 4.8 | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 86.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 364.0 μl of water (100 v/v) |
F2 |
| pH 5.2 | 428.0 μl of Polyethylene glycol 2,000 (50 w/v) 64.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 458.0 μl of water (100 v/v) |
F3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 57.0 μl of Polyethylene glycol 300 (100 v/v) 428.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 465.0 μl of water (100 v/v) |
F4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 428.0 μl of Polyethylene glycol 3,000 (50 w/v) 57.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 465.0 μl of water (100 v/v) |
F5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 358.0 μl of Polyethylene glycol 4,000 (50 w/v) 57.0 μl of Polyethylene glycol 400 (100 v/v) 535.0 μl of water (100 v/v) |
F6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 321.0 μl of Polyethylene glycol 200 (100 v/v) 57.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 572.0 μl of water (100 v/v) |
F7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 286.0 μl of Polyethylene glycol 300 (100 v/v) 114.0 μl of Polyethylene glycol 600 (50 v/v) 550.0 μl of water (100 v/v) |
F8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 286.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 564.0 μl of water (100 v/v) |
F9 |
| pH 7.6 | 100.0 μl of Polyethylene glycol 1,500 (50 w/v) 286.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 564.0 μl of water (100 v/v) |
F10 |
| pH 8 | 86.0 μl of Polyethylene glycol 2,000 (50 w/v) 286.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 578.0 μl of water (100 v/v) |
F11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 286.0 μl of Polyethylene glycol 600 (100 v/v) 86.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 578.0 μl of water (100 v/v) |
F12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 500.0 μl of Polyethylene glycol 1,000 (50 w/v) 86.0 μl of Polyethylene glycol 3,000 (50 w/v) 364.0 μl of water (100 v/v) |
G1 |
| pH 4.8 | 50.0 μl of Polyethylene glycol 1,000 (50 w/v) 514.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 386.0 μl of water (100 v/v) |
G2 |
| pH 5.2 | 50.0 μl of Polyethylene glycol 1,500 (50 w/v) 514.0 μl of Polyethylene glycol 3,000 (50 w/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 386.0 μl of water (100 v/v) |
G3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 42.0 μl of Polyethylene glycol 2,000 (50 w/v) 428.0 μl of Polyethylene glycol 4,000 (50 w/v) 480.0 μl of water (100 v/v) |
G4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 386.0 μl of Polyethylene glycol 200 (100 v/v) 42.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 522.0 μl of water (100 v/v) |
G5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 42.0 μl of Polyethylene glycol 3,000 (50 w/v) 343.0 μl of Polyethylene glycol 300 (100 v/v) 565.0 μl of water (100 v/v) |
G6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 42.0 μl of Polyethylene glycol 4,000 (50 w/v) 343.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 565.0 μl of water (100 v/v) |
G7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 32.0 μl of Polyethylene glycol 200 (100 v/v) 343.0 μl of Polyethylene glycol 400 (100 v/v) 575.0 μl of water (100 v/v) |
G8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 29.0 μl of Polyethylene glycol 300 (100 v/v) 343.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 578.0 μl of water (100 v/v) |
G9 |
| pH 7.6 | 686.0 μl of Polyethylene glycol 600 (50 v/v) 29.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 235.0 μl of water (100 v/v) |
G10 |
| pH 8 | 600.0 μl of Polyethylene glycol 1,000 (50 w/v) 29.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 321.0 μl of water (100 v/v) |
G11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 29.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 321.0 μl of water (100 v/v) |
G12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 514.0 μl of Polyethylene glycol 2,000 (50 w/v) 58.0 μl of Polyethylene glycol 600 (50 v/v) 378.0 μl of water (100 v/v) |
H1 |
| pH 4.8 | 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.8 (1 M) 450.0 μl of water (100 v/v) |
H2 |
| pH 5.2 | 450.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of Sodium acetate pH 5.2 (1 M) 500.0 μl of water (100 v/v) |
H3 |
| pH 5.6 | 50.0 μl of MES pH 5.6 (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 550.0 μl of water (100 v/v) |
H4 |
| pH 6 | 50.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 550.0 μl of water (100 v/v) |
H5 |
| pH 6.4 | 50.0 μl of ADA pH 6.4 (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v) |
H6 |
| pH 6.8 | 50.0 μl of ADA pH 6.8 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 550.0 μl of water (100 v/v) |
H7 |
| pH 7.1 | 50.0 μl of HEPES pH 7.1 (1 M) 800.0 μl of Polyethylene glycol 600 (50 v/v) 150.0 μl of water (100 v/v) |
H8 |
| pH 7.3 | 50.0 μl of HEPES pH 7.3 (1 M) 700.0 μl of Polyethylene glycol 1,000 (50 w/v) 250.0 μl of water (100 v/v) |
H9 |
| pH 7.6 | 700.0 μl of Polyethylene glycol 1,500 (50 w/v) 50.0 μl of tris chloride BCC pH 7.6 (1 M) 250.0 μl of water (100 v/v) |
H10 |
| pH 8 | 600.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of tris chloride BCC pH 8 (1 M) 350.0 μl of water (100 v/v) |
H11 |
| pH 8.4 | 50.0 μl of BICINE pH 8.4 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 350.0 μl of water (100 v/v) |
H12 |
| pH 8.8 | 50.0 μl of BICINE pH 8.8 (1 M) 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 350.0 μl of water (100 v/v) |