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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
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Supplemental Information
Screen Description
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Pi PEG
Barcodes
Pi PEG
Screen Structure
Pi PEG
Well Count | 96 |
---|---|
Number of distinct ingredients | 18 |
Internal diversity (score mean dist) | 0.920 |
Screen Composition
ingredient type | Pi PEG |
---|---|
Buffer | 96 |
Organic (non-volatile) | 28 |
Polymer | 168 |
Salt | 16 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.8 | 225 μl of Polyethylene glycol 200 (100 v/v) 50 μl of Sodium acetate pH 4.8 (1 M) 725 μl of water (100 v/v) |
A2 |
| pH 5.2 | 200 μl of Polyethylene glycol 300 (100 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 750 μl of water (100 v/v) |
A3 |
| pH 5.6 | 50 μl of MES pH 5.6 (1 M) 200 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 750 μl of water (100 v/v) |
A4 |
| pH 6 | 200 μl of Polyethylene glycol 400 (100 v/v) 50 μl of MES pH 6 (1 M) 750 μl of water (100 v/v) |
A5 |
| pH 6.4 | 200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of ADA pH 6.4 (1 M) 750 μl of water (100 v/v) |
A6 |
| pH 6.8 | 50 μl of ADA pH 6.8 (1 M) 400 μl of Polyethylene glycol 600 (50 v/v) 550 μl of water (100 v/v) |
A7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 350 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v) |
A8 |
| pH 7.3 | 350 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of HEPES pH 7.3 (1 M) 600 μl of water (100 v/v) |
A9 |
| pH 7.6 | 50 μl of tris chloride BCC pH 7.6 (1 M) 300 μl of Polyethylene glycol 2,000 (50 w/v) 650 μl of water (100 v/v) |
A10 |
| pH 8 | 50 μl of tris chloride BCC pH 8 (1 M) 300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 650 μl of water (100 v/v) |
A11 |
| pH 8.4 | 50 μl of BICINE pH 8.4 (1 M) 300 μl of Polyethylene glycol 3,000 (50 w/v) 650 μl of water (100 v/v) |
A12 |
| pH 8.8 | 300 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of BICINE pH 8.8 (1 M) 650 μl of water (100 v/v) |
B1 |
| pH 4.8 | 72 μl of Polyethylene glycol 4,000 (50 w/v) 300 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of Sodium acetate pH 4.8 (1 M) 578 μl of water (100 v/v) |
B2 |
| pH 5.2 | 64 μl of Polyethylene glycol 200 (100 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 258 μl of Polyethylene glycol 2,000 (50 w/v) 628 μl of water (100 v/v) |
B3 |
| pH 5.6 | 57 μl of Polyethylene glycol 300 (100 v/v) 50 μl of MES pH 5.6 (1 M) 258 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 635 μl of water (100 v/v) |
B4 |
| pH 6 | 258 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of MES pH 6 (1 M) 57 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 635 μl of water (100 v/v) |
B5 |
| pH 6.4 | 258 μl of Polyethylene glycol 4,000 (50 w/v) 57 μl of Polyethylene glycol 400 (100 v/v) 50 μl of ADA pH 6.4 (1 M) 635 μl of water (100 v/v) |
B6 |
| pH 6.8 | 57 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of ADA pH 6.8 (1 M) 193 μl of Polyethylene glycol 200 (100 v/v) 700 μl of water (100 v/v) |
B7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 171 μl of Polyethylene glycol 300 (100 v/v) 114 μl of Polyethylene glycol 600 (50 v/v) 665 μl of water (100 v/v) |
B8 |
| pH 7.3 | 50 μl of HEPES pH 7.3 (1 M) 100 μl of Polyethylene glycol 1,000 (50 w/v) 171 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 679 μl of water (100 v/v) |
B9 |
| pH 7.6 | 100 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 171 μl of Polyethylene glycol 400 (100 v/v) 679 μl of water (100 v/v) |
B10 |
| pH 8 | 171 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of tris chloride BCC pH 8 (1 M) 86 μl of Polyethylene glycol 2,000 (50 w/v) 693 μl of water (100 v/v) |
B11 |
| pH 8.4 | 50 μl of BICINE pH 8.4 (1 M) 342 μl of Polyethylene glycol 600 (50 v/v) 86 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 522 μl of water (100 v/v) |
B12 |
| pH 8.8 | 86 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of BICINE pH 8.8 (1 M) 300 μl of Polyethylene glycol 1,000 (50 w/v) 564 μl of water (100 v/v) |
C1 |
| pH 4.8 | 114 μl of Polyethylene glycol 300 (100 v/v) 50 μl of Sodium acetate pH 4.8 (1 M) 143 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 693 μl of water (100 v/v) |
C2 |
| pH 5.2 | 143 μl of Polyethylene glycol 400 (100 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 114 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 693 μl of water (100 v/v) |
C3 |
| pH 5.6 | 143 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of MES pH 5.6 (1 M) 114 μl of Polyethylene glycol 400 (100 v/v) 693 μl of water (100 v/v) |
C4 |
| pH 6 | 114 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 286 μl of Polyethylene glycol 600 (50 v/v) 50 μl of MES pH 6 (1 M) 550 μl of water (100 v/v) |
C5 |
| pH 6.4 | 228 μl of Polyethylene glycol 600 (50 v/v) 250 μl of Polyethylene glycol 1,000 (50 w/v) 50 μl of ADA pH 6.4 (1 M) 472 μl of water (100 v/v) |
C6 |
| pH 6.8 | 50 μl of ADA pH 6.8 (1 M) 250 μl of Polyethylene glycol 1,500 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 500 μl of water (100 v/v) |
C7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 200 μl of Polyethylene glycol 1,500 (50 w/v) 214 μl of Polyethylene glycol 2,000 (50 w/v) 536 μl of water (100 v/v) |
C8 |
| pH 7.3 | 50 μl of HEPES pH 7.3 (1 M) 214 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 172 μl of Polyethylene glycol 2,000 (50 w/v) 564 μl of water (100 v/v) |
C9 |
| pH 7.6 | 214 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 172 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 564 μl of water (100 v/v) |
C10 |
| pH 8 | 214 μl of Polyethylene glycol 4,000 (50 w/v) 172 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of tris chloride BCC pH 8 (1 M) 564 μl of water (100 v/v) |
C11 |
| pH 8.4 | 142 μl of Polyethylene glycol 4,000 (50 w/v) 161 μl of Polyethylene glycol 200 (100 v/v) 50 μl of BICINE pH 8.4 (1 M) 647 μl of water (100 v/v) |
C12 |
| pH 8.8 | 129 μl of Polyethylene glycol 200 (100 v/v) 143 μl of Polyethylene glycol 300 (100 v/v) 50 μl of BICINE pH 8.8 (1 M) 678 μl of water (100 v/v) |
D1 |
| pH 4.8 | 50 μl of Sodium acetate pH 4.8 (1 M) 171 μl of Polyethylene glycol 400 (100 v/v) 172 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 607 μl of water (100 v/v) |
D2 |
| pH 5.2 | 171 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 172 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of Sodium acetate pH 5.2 (1 M) 607 μl of water (100 v/v) |
D3 |
| pH 5.6 | 172 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of MES pH 5.6 (1 M) 342 μl of Polyethylene glycol 600 (50 v/v) 436 μl of water (100 v/v) |
D4 |
| pH 6 | 129 μl of Polyethylene glycol 200 (100 v/v) 300 μl of Polyethylene glycol 1,000 (50 w/v) 50 μl of MES pH 6 (1 M) 521 μl of water (100 v/v) |
D5 |
| pH 6.4 | 300 μl of Polyethylene glycol 1,500 (50 w/v) 114 μl of Polyethylene glycol 300 (100 v/v) 50 μl of ADA pH 6.4 (1 M) 536 μl of water (100 v/v) |
D6 |
| pH 6.8 | 50 μl of ADA pH 6.8 (1 M) 114 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 258 μl of Polyethylene glycol 2,000 (50 w/v) 578 μl of water (100 v/v) |
D7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 114 μl of Polyethylene glycol 400 (100 v/v) 258 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 578 μl of water (100 v/v) |
D8 |
| pH 7.3 | 114 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 258 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of HEPES pH 7.3 (1 M) 578 μl of water (100 v/v) |
D9 |
| pH 7.6 | 214 μl of Polyethylene glycol 4,000 (50 w/v) 228 μl of Polyethylene glycol 600 (50 v/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 508 μl of water (100 v/v) |
D10 |
| pH 8 | 193 μl of Polyethylene glycol 200 (100 v/v) 50 μl of tris chloride BCC pH 8 (1 M) 200 μl of Polyethylene glycol 1,000 (50 w/v) 557 μl of water (100 v/v) |
D11 |
| pH 8.4 | 200 μl of Polyethylene glycol 1,500 (50 w/v) 171 μl of Polyethylene glycol 300 (100 v/v) 50 μl of BICINE pH 8.4 (1 M) 579 μl of water (100 v/v) |
D12 |
| pH 8.8 | 50 μl of BICINE pH 8.8 (1 M) 171 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 172 μl of Polyethylene glycol 2,000 (50 w/v) 607 μl of water (100 v/v) |
E1 |
| pH 4.8 | 86 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 458 μl of Polyethylene glycol 600 (50 v/v) 50 μl of Sodium acetate pH 4.8 (1 M) 406 μl of water (100 v/v) |
E2 |
| pH 5.2 | 172 μl of Polyethylene glycol 600 (50 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 378 μl of water (100 v/v) |
E3 |
| pH 5.6 | 400 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of MES pH 5.6 (1 M) 150 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
E4 |
| pH 6 | 150 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of MES pH 6 (1 M) 342 μl of Polyethylene glycol 2,000 (50 w/v) 458 μl of water (100 v/v) |
E5 |
| pH 6.4 | 342 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50 μl of ADA pH 6.4 (1 M) 128 μl of Polyethylene glycol 2,000 (50 w/v) 480 μl of water (100 v/v) |
E6 |
| pH 6.8 | 50 μl of ADA pH 6.8 (1 M) 342 μl of Polyethylene glycol 3,000 (50 w/v) 128 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 480 μl of water (100 v/v) |
E7 |
| pH 7.1 | 286 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of HEPES pH 7.1 (1 M) 128 μl of Polyethylene glycol 3,000 (50 w/v) 536 μl of water (100 v/v) |
E8 |
| pH 7.3 | 128 μl of Polyethylene glycol 4,000 (50 w/v) 257 μl of Polyethylene glycol 200 (100 v/v) 50 μl of HEPES pH 7.3 (1 M) 565 μl of water (100 v/v) |
E9 |
| pH 7.6 | 96 μl of Polyethylene glycol 200 (100 v/v) 229 μl of Polyethylene glycol 300 (100 v/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 625 μl of water (100 v/v) |
E10 |
| pH 8 | 86 μl of Polyethylene glycol 300 (100 v/v) 50 μl of tris chloride BCC pH 8 (1 M) 229 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 635 μl of water (100 v/v) |
E11 |
| pH 8.4 | 50 μl of BICINE pH 8.4 (1 M) 229 μl of Polyethylene glycol 400 (100 v/v) 86 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 635 μl of water (100 v/v) |
E12 |
| pH 8.8 | 229 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of BICINE pH 8.8 (1 M) 86 μl of Polyethylene glycol 400 (100 v/v) 635 μl of water (100 v/v) |
F1 |
| pH 4.8 | 86 μl of Polyethylene glycol 4,000 (50 w/v) 500 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of Sodium acetate pH 4.8 (1 M) 364 μl of water (100 v/v) |
F2 |
| pH 5.2 | 64 μl of Polyethylene glycol 200 (100 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 428 μl of Polyethylene glycol 2,000 (50 w/v) 458 μl of water (100 v/v) |
F3 |
| pH 5.6 | 57 μl of Polyethylene glycol 300 (100 v/v) 50 μl of MES pH 5.6 (1 M) 428 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 465 μl of water (100 v/v) |
F4 |
| pH 6 | 428 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of MES pH 6 (1 M) 57 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 465 μl of water (100 v/v) |
F5 |
| pH 6.4 | 358 μl of Polyethylene glycol 4,000 (50 w/v) 57 μl of Polyethylene glycol 400 (100 v/v) 50 μl of ADA pH 6.4 (1 M) 535 μl of water (100 v/v) |
F6 |
| pH 6.8 | 57 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of ADA pH 6.8 (1 M) 321 μl of Polyethylene glycol 200 (100 v/v) 572 μl of water (100 v/v) |
F7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 286 μl of Polyethylene glycol 300 (100 v/v) 114 μl of Polyethylene glycol 600 (50 v/v) 550 μl of water (100 v/v) |
F8 |
| pH 7.3 | 50 μl of HEPES pH 7.3 (1 M) 100 μl of Polyethylene glycol 1,000 (50 w/v) 286 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 564 μl of water (100 v/v) |
F9 |
| pH 7.6 | 100 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 286 μl of Polyethylene glycol 400 (100 v/v) 564 μl of water (100 v/v) |
F10 |
| pH 8 | 286 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of tris chloride BCC pH 8 (1 M) 86 μl of Polyethylene glycol 2,000 (50 w/v) 578 μl of water (100 v/v) |
F11 |
| pH 8.4 | 50 μl of BICINE pH 8.4 (1 M) 86 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 286 μl of Polyethylene glycol 600 (100 v/v) 578 μl of water (100 v/v) |
F12 |
| pH 8.8 | 86 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of BICINE pH 8.8 (1 M) 500 μl of Polyethylene glycol 1,000 (50 w/v) 364 μl of water (100 v/v) |
G1 |
| pH 4.8 | 50 μl of Sodium acetate pH 4.8 (1 M) 514 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50 μl of Polyethylene glycol 1,000 (50 w/v) 386 μl of water (100 v/v) |
G2 |
| pH 5.2 | 50 μl of Polyethylene glycol 1,500 (50 w/v) 514 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of Sodium acetate pH 5.2 (1 M) 386 μl of water (100 v/v) |
G3 |
| pH 5.6 | 428 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of MES pH 5.6 (1 M) 42 μl of Polyethylene glycol 2,000 (50 w/v) 480 μl of water (100 v/v) |
G4 |
| pH 6 | 386 μl of Polyethylene glycol 200 (100 v/v) 42 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50 μl of MES pH 6 (1 M) 522 μl of water (100 v/v) |
G5 |
| pH 6.4 | 343 μl of Polyethylene glycol 300 (100 v/v) 42 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of ADA pH 6.4 (1 M) 565 μl of water (100 v/v) |
G6 |
| pH 6.8 | 50 μl of ADA pH 6.8 (1 M) 42 μl of Polyethylene glycol 4,000 (50 w/v) 343 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 565 μl of water (100 v/v) |
G7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 32 μl of Polyethylene glycol 200 (100 v/v) 343 μl of Polyethylene glycol 400 (100 v/v) 575 μl of water (100 v/v) |
G8 |
| pH 7.3 | 343 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 29 μl of Polyethylene glycol 300 (100 v/v) 50 μl of HEPES pH 7.3 (1 M) 578 μl of water (100 v/v) |
G9 |
| pH 7.6 | 686 μl of Polyethylene glycol 600 (50 v/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 29 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 235 μl of water (100 v/v) |
G10 |
| pH 8 | 29 μl of Polyethylene glycol 400 (100 v/v) 50 μl of tris chloride BCC pH 8 (1 M) 600 μl of Polyethylene glycol 1,000 (50 w/v) 321 μl of water (100 v/v) |
G11 |
| pH 8.4 | 29 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 600 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of BICINE pH 8.4 (1 M) 321 μl of water (100 v/v) |
G12 |
| pH 8.8 | 58 μl of Polyethylene glycol 600 (50 v/v) 50 μl of BICINE pH 8.8 (1 M) 514 μl of Polyethylene glycol 2,000 (50 w/v) 378 μl of water (100 v/v) |
H1 |
| pH 4.8 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium acetate pH 4.8 (1 M) 450 μl of water (100 v/v) |
H2 |
| pH 5.2 | 450 μl of Polyethylene glycol 200 (100 v/v) 50 μl of Sodium acetate pH 5.2 (1 M) 500 μl of water (100 v/v) |
H3 |
| pH 5.6 | 400 μl of Polyethylene glycol 300 (100 v/v) 50 μl of MES pH 5.6 (1 M) 550 μl of water (100 v/v) |
H4 |
| pH 6 | 50 μl of MES pH 6 (1 M) 400 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 550 μl of water (100 v/v) |
H5 |
| pH 6.4 | 400 μl of Polyethylene glycol 400 (100 v/v) 50 μl of ADA pH 6.4 (1 M) 550 μl of water (100 v/v) |
H6 |
| pH 6.8 | 400 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 50 μl of ADA pH 6.8 (1 M) 550 μl of water (100 v/v) |
H7 |
| pH 7.1 | 50 μl of HEPES pH 7.1 (1 M) 800 μl of Polyethylene glycol 600 (50 v/v) 150 μl of water (100 v/v) |
H8 |
| pH 7.3 | 50 μl of HEPES pH 7.3 (1 M) 700 μl of Polyethylene glycol 1,000 (50 w/v) 250 μl of water (100 v/v) |
H9 |
| pH 7.6 | 700 μl of Polyethylene glycol 1,500 (50 w/v) 50 μl of tris chloride BCC pH 7.6 (1 M) 250 μl of water (100 v/v) |
H10 |
| pH 8 | 50 μl of tris chloride BCC pH 8 (1 M) 600 μl of Polyethylene glycol 2,000 (50 w/v) 350 μl of water (100 v/v) |
H11 |
| pH 8.4 | 50 μl of BICINE pH 8.4 (1 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 350 μl of water (100 v/v) |
H12 |
| pH 8.8 | 600 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of BICINE pH 8.8 (1 M) 350 μl of water (100 v/v) |