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Supplemental Information

Screen Description

Pi PEG

Pi screens / Pi PEG
Created 2011-06-15.

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Pi PEG

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Screen Structure

Pi PEG

Well Count 96
Number of distinct ingredients 18
Internal diversity (score mean dist) 0.920

Screen Composition

2025-08-16T04:27:14.308492 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Pi PEG
Buffer 96
Organic (non-volatile) 28
Polymer 168
Salt 16

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Pi PEG Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 200 22.5 v/v -
  • Sodium acetate 0.05 M pH 4.8
pH 4.8
225.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
725.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 300 20 v/v -
  • Sodium acetate 0.05 M pH 5.2
pH 5.2
200.0 μl of Polyethylene glycol 300 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
750.0 μl of water (100 v/v)
A3
  • Polyethylene glycol monomethyl ether 350 20 v/v -
  • MES 0.05 M pH 5.6
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
750.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 400 20 v/v -
  • MES 0.05 M pH 6
pH 6
50.0 μl of MES pH 6 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
750.0 μl of water (100 v/v)
A5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • ADA 0.05 M pH 6.4
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
750.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 600 20 v/v -
  • ADA 0.05 M pH 6.8
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
400.0 μl of Polyethylene glycol 600 (50 v/v)
550.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 1,000 17.5 w/v -
  • HEPES 0.05 M pH 7.1
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
350.0 μl of Polyethylene glycol 1,000 (50 w/v)
600.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 1,500 17.5 w/v -
  • HEPES 0.05 M pH 7.3
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
350.0 μl of Polyethylene glycol 1,500 (50 w/v)
600.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 2,000 15 w/v -
  • tris chloride BCC 0.05 M pH 7.6
pH 7.6
300.0 μl of Polyethylene glycol 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
650.0 μl of water (100 v/v)
A10
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • tris chloride BCC 0.05 M pH 8
pH 8
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
650.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 3,000 15 w/v -
  • BICINE 0.05 M pH 8.4
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
300.0 μl of Polyethylene glycol 3,000 (50 w/v)
650.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 4,000 15 w/v -
  • BICINE 0.05 M pH 8.8
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 1,500 15 w/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol 4,000 3.6 w/v -
pH 4.8
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
72.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
578.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 2,000 12.9 w/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol 200 6.4 v/v -
pH 5.2
258.0 μl of Polyethylene glycol 2,000 (50 w/v)
64.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
628.0 μl of water (100 v/v)
B3
  • Polyethylene glycol monomethyl ether 2,000 12.9 w/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol 300 5.7 v/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
57.0 μl of Polyethylene glycol 300 (100 v/v)
258.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
635.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,000 12.9 w/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol monomethyl ether 350 5.7 v/v -
pH 6
50.0 μl of MES pH 6 (1 M)
258.0 μl of Polyethylene glycol 3,000 (50 w/v)
57.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
635.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 4,000 12.9 w/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol 400 5.7 v/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
258.0 μl of Polyethylene glycol 4,000 (50 w/v)
57.0 μl of Polyethylene glycol 400 (100 v/v)
635.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 200 19.3 v/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol monomethyl ether 550 5.7 v/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
193.0 μl of Polyethylene glycol 200 (100 v/v)
57.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
700.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 300 17.1 v/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol 600 5.7 v/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
171.0 μl of Polyethylene glycol 300 (100 v/v)
114.0 μl of Polyethylene glycol 600 (50 v/v)
665.0 μl of water (100 v/v)
B8
  • Polyethylene glycol monomethyl ether 350 17.1 v/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol 1,000 5 w/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
171.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
679.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 400 17.1 v/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol 1,500 5 w/v -
pH 7.6
100.0 μl of Polyethylene glycol 1,500 (50 w/v)
171.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
679.0 μl of water (100 v/v)
B10
  • Polyethylene glycol monomethyl ether 550 17.1 v/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 2,000 4.3 w/v -
pH 8
86.0 μl of Polyethylene glycol 2,000 (50 w/v)
171.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
693.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 600 17.1 v/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol monomethyl ether 2,000 4.3 w/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
342.0 μl of Polyethylene glycol 600 (50 v/v)
86.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
522.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 1,000 15 w/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol 3,000 4.3 w/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
86.0 μl of Polyethylene glycol 3,000 (50 w/v)
564.0 μl of water (100 v/v)
C1
  • Polyethylene glycol monomethyl ether 350 14.3 v/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol 300 11.4 v/v -
pH 4.8
114.0 μl of Polyethylene glycol 300 (100 v/v)
143.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
693.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 400 14.3 v/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol monomethyl ether 350 11.4 v/v -
pH 5.2
143.0 μl of Polyethylene glycol 400 (100 v/v)
114.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
693.0 μl of water (100 v/v)
C3
  • Polyethylene glycol monomethyl ether 550 14.3 v/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol 400 11.4 v/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
114.0 μl of Polyethylene glycol 400 (100 v/v)
143.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
693.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 600 14.3 v/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol monomethyl ether 550 11.4 v/v -
pH 6
50.0 μl of MES pH 6 (1 M)
286.0 μl of Polyethylene glycol 600 (50 v/v)
114.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
550.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 1,000 12.5 w/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol 600 11.4 v/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
250.0 μl of Polyethylene glycol 1,000 (50 w/v)
228.0 μl of Polyethylene glycol 600 (50 v/v)
472.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 1,500 12.5 w/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol 1,000 10 w/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
250.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 2,000 10.7 w/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol 1,500 10 w/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
200.0 μl of Polyethylene glycol 1,500 (50 w/v)
214.0 μl of Polyethylene glycol 2,000 (50 w/v)
536.0 μl of water (100 v/v)
C8
  • Polyethylene glycol monomethyl ether 2,000 10.7 w/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol 2,000 8.6 w/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
172.0 μl of Polyethylene glycol 2,000 (50 w/v)
214.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
564.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 3,000 10.7 w/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol monomethyl ether 2,000 8.6 w/v -
pH 7.6
214.0 μl of Polyethylene glycol 3,000 (50 w/v)
172.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
564.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 4,000 10.7 w/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 3,000 8.6 w/v -
pH 8
172.0 μl of Polyethylene glycol 3,000 (50 w/v)
214.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
564.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 200 16.1 v/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol 4,000 7.1 w/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
161.0 μl of Polyethylene glycol 200 (100 v/v)
142.0 μl of Polyethylene glycol 4,000 (50 w/v)
647.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 300 14.3 v/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol 200 12.9 v/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
129.0 μl of Polyethylene glycol 200 (100 v/v)
143.0 μl of Polyethylene glycol 300 (100 v/v)
678.0 μl of water (100 v/v)
D1
  • Polyethylene glycol monomethyl ether 2,000 8.6 w/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol 400 17.1 v/v -
pH 4.8
171.0 μl of Polyethylene glycol 400 (100 v/v)
172.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
607.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 3,000 8.6 w/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol monomethyl ether 550 17.1 v/v -
pH 5.2
172.0 μl of Polyethylene glycol 3,000 (50 w/v)
171.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
607.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 4,000 8.6 w/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol 600 17.1 v/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
172.0 μl of Polyethylene glycol 4,000 (50 w/v)
342.0 μl of Polyethylene glycol 600 (50 v/v)
436.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 200 12.9 v/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol 1,000 15 w/v -
pH 6
50.0 μl of MES pH 6 (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
129.0 μl of Polyethylene glycol 200 (100 v/v)
521.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 300 11.4 v/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol 1,500 15 w/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
114.0 μl of Polyethylene glycol 300 (100 v/v)
536.0 μl of water (100 v/v)
D6
  • Polyethylene glycol monomethyl ether 350 11.4 v/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol 2,000 12.9 w/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
258.0 μl of Polyethylene glycol 2,000 (50 w/v)
114.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
578.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 400 11.4 v/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol monomethyl ether 2,000 12.9 w/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
114.0 μl of Polyethylene glycol 400 (100 v/v)
258.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
578.0 μl of water (100 v/v)
D8
  • Polyethylene glycol monomethyl ether 550 11.4 v/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol 3,000 12.9 w/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
258.0 μl of Polyethylene glycol 3,000 (50 w/v)
114.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
578.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 600 11.4 v/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol 4,000 10.7 w/v -
pH 7.6
214.0 μl of Polyethylene glycol 4,000 (50 w/v)
228.0 μl of Polyethylene glycol 600 (50 v/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
508.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 1,000 10 w/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 200 19.3 v/v -
pH 8
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
193.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
557.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 1,500 10 w/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol 300 17.1 v/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
200.0 μl of Polyethylene glycol 1,500 (50 w/v)
171.0 μl of Polyethylene glycol 300 (100 v/v)
579.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 2,000 8.6 w/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol monomethyl ether 350 17.1 v/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
172.0 μl of Polyethylene glycol 2,000 (50 w/v)
171.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
607.0 μl of water (100 v/v)
E1
  • Polyethylene glycol monomethyl ether 550 8.6 v/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol 600 22.9 v/v -
pH 4.8
458.0 μl of Polyethylene glycol 600 (50 v/v)
86.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
406.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 600 8.6 v/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol 1,000 20 w/v -
pH 5.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
172.0 μl of Polyethylene glycol 600 (50 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
378.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 1,000 7.5 w/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol 1,500 20 w/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
150.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 1,500 7.5 w/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol 2,000 17.1 w/v -
pH 6
50.0 μl of MES pH 6 (1 M)
150.0 μl of Polyethylene glycol 1,500 (50 w/v)
342.0 μl of Polyethylene glycol 2,000 (50 w/v)
458.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 2,000 6.4 w/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol monomethyl ether 2,000 17.1 w/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
128.0 μl of Polyethylene glycol 2,000 (50 w/v)
342.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
480.0 μl of water (100 v/v)
E6
  • Polyethylene glycol monomethyl ether 2,000 6.4 w/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol 3,000 17.1 w/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
342.0 μl of Polyethylene glycol 3,000 (50 w/v)
128.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
480.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 3,000 6.4 w/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol 4,000 14.3 w/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
128.0 μl of Polyethylene glycol 3,000 (50 w/v)
286.0 μl of Polyethylene glycol 4,000 (50 w/v)
536.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 4,000 6.4 w/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol 200 25.7 v/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
257.0 μl of Polyethylene glycol 200 (100 v/v)
128.0 μl of Polyethylene glycol 4,000 (50 w/v)
565.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 200 9.6 v/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol 300 22.9 v/v -
pH 7.6
96.0 μl of Polyethylene glycol 200 (100 v/v)
229.0 μl of Polyethylene glycol 300 (100 v/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
625.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 300 8.6 v/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol monomethyl ether 350 22.9 v/v -
pH 8
86.0 μl of Polyethylene glycol 300 (100 v/v)
229.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
635.0 μl of water (100 v/v)
E11
  • Polyethylene glycol monomethyl ether 350 8.6 v/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol 400 22.9 v/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
229.0 μl of Polyethylene glycol 400 (100 v/v)
86.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
635.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 400 8.6 v/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol monomethyl ether 550 22.9 v/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
86.0 μl of Polyethylene glycol 400 (100 v/v)
229.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
635.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 4,000 4.3 w/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol 1,500 25 w/v -
pH 4.8
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
86.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
364.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 200 6.4 v/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol 2,000 21.4 w/v -
pH 5.2
428.0 μl of Polyethylene glycol 2,000 (50 w/v)
64.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
458.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 300 5.7 v/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol monomethyl ether 2,000 21.4 w/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
57.0 μl of Polyethylene glycol 300 (100 v/v)
428.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
465.0 μl of water (100 v/v)
F4
  • Polyethylene glycol monomethyl ether 350 5.7 v/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol 3,000 21.4 w/v -
pH 6
50.0 μl of MES pH 6 (1 M)
428.0 μl of Polyethylene glycol 3,000 (50 w/v)
57.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
465.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 400 5.7 v/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol 4,000 17.9 w/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
358.0 μl of Polyethylene glycol 4,000 (50 w/v)
57.0 μl of Polyethylene glycol 400 (100 v/v)
535.0 μl of water (100 v/v)
F6
  • Polyethylene glycol monomethyl ether 550 5.7 v/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol 200 32.1 v/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
321.0 μl of Polyethylene glycol 200 (100 v/v)
57.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
572.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 600 5.7 v/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol 300 28.6 v/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
286.0 μl of Polyethylene glycol 300 (100 v/v)
114.0 μl of Polyethylene glycol 600 (50 v/v)
550.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 1,000 5 w/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol monomethyl ether 350 28.6 v/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
286.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
564.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 1,500 5 w/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol 400 28.6 v/v -
pH 7.6
100.0 μl of Polyethylene glycol 1,500 (50 w/v)
286.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
564.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 2,000 4.3 w/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol monomethyl ether 550 28.6 v/v -
pH 8
86.0 μl of Polyethylene glycol 2,000 (50 w/v)
286.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
578.0 μl of water (100 v/v)
F11
  • Polyethylene glycol monomethyl ether 2,000 4.3 w/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol 600 28.6 v/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
286.0 μl of Polyethylene glycol 600 (100 v/v)
86.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
578.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,000 4.3 w/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol 1,000 25 w/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
86.0 μl of Polyethylene glycol 3,000 (50 w/v)
364.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 1,000 2.5 w/v -
  • Sodium acetate 0.05 M pH 4.8
  • Polyethylene glycol monomethyl ether 2,000 25.7 w/v -
pH 4.8
50.0 μl of Polyethylene glycol 1,000 (50 w/v)
514.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
386.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 1,500 2.5 w/v -
  • Sodium acetate 0.05 M pH 5.2
  • Polyethylene glycol 3,000 25.7 w/v -
pH 5.2
50.0 μl of Polyethylene glycol 1,500 (50 w/v)
514.0 μl of Polyethylene glycol 3,000 (50 w/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
386.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 2,000 2.1 w/v -
  • MES 0.05 M pH 5.6
  • Polyethylene glycol 4,000 21.4 w/v -
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
42.0 μl of Polyethylene glycol 2,000 (50 w/v)
428.0 μl of Polyethylene glycol 4,000 (50 w/v)
480.0 μl of water (100 v/v)
G4
  • Polyethylene glycol monomethyl ether 2,000 2.1 w/v -
  • MES 0.05 M pH 6
  • Polyethylene glycol 200 38.6 v/v -
pH 6
50.0 μl of MES pH 6 (1 M)
386.0 μl of Polyethylene glycol 200 (100 v/v)
42.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
522.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,000 2.1 w/v -
  • ADA 0.05 M pH 6.4
  • Polyethylene glycol 300 34.3 v/v -
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
42.0 μl of Polyethylene glycol 3,000 (50 w/v)
343.0 μl of Polyethylene glycol 300 (100 v/v)
565.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 4,000 2.1 w/v -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol monomethyl ether 350 34.3 v/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
42.0 μl of Polyethylene glycol 4,000 (50 w/v)
343.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
565.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 200 3.2 v/v -
  • HEPES 0.05 M pH 7.1
  • Polyethylene glycol 400 34.3 v/v -
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
32.0 μl of Polyethylene glycol 200 (100 v/v)
343.0 μl of Polyethylene glycol 400 (100 v/v)
575.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 300 2.9 v/v -
  • HEPES 0.05 M pH 7.3
  • Polyethylene glycol monomethyl ether 550 34.3 v/v -
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
29.0 μl of Polyethylene glycol 300 (100 v/v)
343.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
578.0 μl of water (100 v/v)
G9
  • Polyethylene glycol monomethyl ether 350 2.9 v/v -
  • tris chloride BCC 0.05 M pH 7.6
  • Polyethylene glycol 600 34.3 v/v -
pH 7.6
686.0 μl of Polyethylene glycol 600 (50 v/v)
29.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
235.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 400 2.9 v/v -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 1,000 30 w/v -
pH 8
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
29.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
321.0 μl of water (100 v/v)
G11
  • Polyethylene glycol monomethyl ether 550 2.9 v/v -
  • BICINE 0.05 M pH 8.4
  • Polyethylene glycol 1,500 30 w/v -
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
29.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
321.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 600 2.9 v/v -
  • BICINE 0.05 M pH 8.8
  • Polyethylene glycol 2,000 25.7 w/v -
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
514.0 μl of Polyethylene glycol 2,000 (50 w/v)
58.0 μl of Polyethylene glycol 600 (50 v/v)
378.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.05 M pH 4.8
pH 4.8
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.8 (1 M)
450.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 200 45 v/v -
  • Sodium acetate 0.05 M pH 5.2
pH 5.2
450.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium acetate pH 5.2 (1 M)
500.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 300 40 v/v -
  • MES 0.05 M pH 5.6
pH 5.6
50.0 μl of MES pH 5.6 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
550.0 μl of water (100 v/v)
H4
  • Polyethylene glycol monomethyl ether 350 40 v/v -
  • MES 0.05 M pH 6
pH 6
50.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
550.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 400 40 v/v -
  • ADA 0.05 M pH 6.4
pH 6.4
50.0 μl of ADA pH 6.4 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
H6
  • Polyethylene glycol monomethyl ether 550 40 v/v -
  • ADA 0.05 M pH 6.8
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
550.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 600 40 v/v -
  • HEPES 0.05 M pH 7.1
pH 7.1
50.0 μl of HEPES pH 7.1 (1 M)
800.0 μl of Polyethylene glycol 600 (50 v/v)
150.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 1,000 35 w/v -
  • HEPES 0.05 M pH 7.3
pH 7.3
50.0 μl of HEPES pH 7.3 (1 M)
700.0 μl of Polyethylene glycol 1,000 (50 w/v)
250.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 1,500 35 w/v -
  • tris chloride BCC 0.05 M pH 7.6
pH 7.6
700.0 μl of Polyethylene glycol 1,500 (50 w/v)
50.0 μl of tris chloride BCC pH 7.6 (1 M)
250.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 2,000 30 w/v -
  • tris chloride BCC 0.05 M pH 8
pH 8
600.0 μl of Polyethylene glycol 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
350.0 μl of water (100 v/v)
H11
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • BICINE 0.05 M pH 8.4
pH 8.4
50.0 μl of BICINE pH 8.4 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
350.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 3,000 30 w/v -
  • BICINE 0.05 M pH 8.8
pH 8.8
50.0 μl of BICINE pH 8.8 (1 M)
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
350.0 μl of water (100 v/v)