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Supplemental Information

Screen Description

MCSG-1

Created 2011-06-15.

Export

Barcodes

MCSG-1

No barcodes found.

Screen Structure

MCSG-1

Well Count 96
Number of distinct ingredients 57
Internal diversity (score mean dist) 0.911

Screen Composition

2025-08-16T09:05:43.212098 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MCSG-1
Buffer 96
Cryoprotectant 6
Organic (non-volatile) 11
Organic (volatile) 1
Polymer 78
Salt 99

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MCSG-1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 8,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
A2
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 3,000 30 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
300.0 μl of water (100 v/v)
A3
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 8,000 10 w/v -
pH 6.2
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
726.7 μl of water (100 v/v)
A4
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Sodium chloride 2.5 M -
pH 7
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Sodium chloride (5 M)
33.3 μl of tris chloride BCC pH 7 (3 M)
366.7 μl of water (100 v/v)
A5
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 1.26 M -
pH 4.5
360.0 μl of Ammonium sulfate (3.5 M)
50.0 μl of Sodium acetate pH 4.5 (2 M)
40.0 μl of Sodium chloride (5 M)
550.0 μl of water (100 v/v)
A6
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
A7
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
A8
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
566.7 μl of water (100 v/v)
A9
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
A10
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polypropylene glycol 400 28 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polypropylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
A11
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 10 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
A12
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B1
  • Sodium chloride 0.6 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
120.0 μl of Sodium chloride (5 M)
380.0 μl of water (100 v/v)
B2
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
B3
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
B4
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B6
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 8,000 20 w/v -
pH 6
200.0 μl of Calcium acetate (1 M)
33.3 μl of MES pH 6 (3 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
366.7 μl of water (100 v/v)
B7
  • Ammonium acetate 0.17 M -
  • tri-Sodium citrate 0.085 M pH 5.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 5.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
85.0 μl of water (100 v/v)
B8
  • Sodium acetate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
56.7 μl of Sodium acetate (3 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
198.3 μl of water (100 v/v)
B9
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
B10
  • Magnesium chloride 0.16 M -
  • tris chloride BCC 0.08 M pH 8.5
  • Polyethylene glycol 4,000 24 w/v -
  • Glycerol 20 v/v -
pH 8.5
200.0 μl of Glycerol (100 v/v)
80.0 μl of Magnesium chloride (2 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
160.0 μl of water (100 v/v)
B11
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 20 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B12
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
C1
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
C2
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
C3
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate pH 5.9 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C4
  • Ammonium acetate 0.17 M -
  • Sodium acetate 0.085 M pH 4.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 4.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate pH 4.6 (1 M)
85.0 μl of water (100 v/v)
C5
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C6
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polypropylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polypropylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
C7
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
100.0 μl of Calcium chloride (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C8
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 25 w/v -
pH 5.6
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
342.9 μl of water (100 v/v)
C9
  • Lithium chloride 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 32 w/v -
pH 8.5
80.0 μl of Lithium chloride (10 M)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
180.0 μl of water (100 v/v)
C10
  • Lithium chloride 1 M -
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 6,000 30 w/v -
NA
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
266.7 μl of water (100 v/v)
C11
  • Calcium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,000 20 w/v -
pH 7
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
366.7 μl of water (100 v/v)
C12
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D1
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
D2
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
D3
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
D4
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
D5
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium formate pH 6.6 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
D6
  • di-Sodium malonate 1.4 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
411.8 μl of di-Sodium malonate pH 7 (3.4 M)
488.2 μl of water (100 v/v)
D7
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
D8
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 1.5 M -
pH 6.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
20.0 μl of Sodium chloride (5 M)
451.4 μl of water (100 v/v)
D9
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
D10
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
D11
  • Sodium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium iodide (1 M)
400.0 μl of water (100 v/v)
D12
  • Ammonium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Ammonium chloride pH 6.3 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
E1
  • Ammonium sulfate 2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
328.6 μl of water (100 v/v)
E2
  • Calcium chloride 0.05 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
E3
  • Magnesium chloride 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
25.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
E4
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
E5
  • Magnesium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
E6
  • Potassium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of Potassium sulfate (0.5 M)
200.0 μl of water (100 v/v)
E7
  • Ammonium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Ammonium iodide (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E8
  • Magnesium sulfate 1.8 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
900.0 μl of Magnesium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
E9
  • Calcium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E10
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
E11
  • Sodium Potassium phosphate 1 M pH 8.2
pH 8.2
333.3 μl of Sodium Potassium phosphate pH 8.2 (3 M)
666.7 μl of water (100 v/v)
E12
  • di-Sodium malonate 2.4 M -
NA
705.9 μl of di-Sodium malonate pH 7 (3.4 M)
294.1 μl of water (100 v/v)
F1
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
F2
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
F3
  • di-Ammonium hydrogen citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M)
520.0 μl of water (100 v/v)
F4
  • Magnesium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
F5
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
533.3 μl of water (100 v/v)
F6
  • Potassium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
F7
  • Sodium Potassium phosphate 1 M pH 5.6
pH 5.6
271.6 μl of Sodium Potassium phosphate pH 4.6 (3 M)
61.8 μl of Sodium Potassium phosphate pH 8.8 (3 M)
666.7 μl of water (100 v/v)
F8
  • Sodium formate 3.5 M -
NA
500.0 μl of Sodium formate pH 7 (7 M)
500.0 μl of water (100 v/v)
F9
  • Ammonium sulfate 0.05 M -
  • BIS-TRIS 0.05 M pH 6.5
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
pH 6.5
14.3 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
335.7 μl of water (100 v/v)
F10
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
F11
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F12
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
G1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 10 w/v -
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
G2
  • Potassium di-hydrogen phosphate 0.04 M -
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
26.7 μl of Potassium di-hydrogen phosphate (1.5 M)
453.3 μl of water (100 v/v)
G3
  • BIS-TRIS propane 0.1 M pH 7
  • Ammonium sulfate 1.5 M -
pH 7
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS propane pH 7 (1 M)
471.4 μl of water (100 v/v)
G4
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
G5
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
328.6 μl of water (100 v/v)
G6
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 25 w/v -
pH 4.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
450.0 μl of water (100 v/v)
G7
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
G9
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
G10
  • Polyethylene glycol 3,350 15 w/v -
  • Magnesium formate 0.1 M -
NA
100.0 μl of Magnesium formate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
G11
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
G12
  • Calcium acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 14.4 w/v -
  • Glycerol 20 v/v -
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
272.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium fluoride 0.2 M -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium iodide 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium iodide (1 M)
400.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium acetate 0.2 M -
NA
40.0 μl of Lithium acetate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
H4
  • tri-Sodium citrate 0.1 M pH 5.6
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium chloride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
H6
  • Sodium acetate 0.1 M pH 4.6
  • Sodium formate 3.5 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of Sodium formate (7 M)
400.0 μl of water (100 v/v)
H7
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
328.6 μl of water (100 v/v)
H8
  • di-Sodium succinate 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
833.3 μl of di-Sodium succinate pH 7 (1.2 M)
46.7 μl of water (100 v/v)
H9
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
H10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
H11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
H12
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)