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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
MCSG-1
Barcodes
MCSG-1
Screen Structure
MCSG-1
Well Count | 96 |
---|---|
Number of distinct ingredients | 57 |
Internal diversity (score mean dist) | 0.911 |
Screen Composition
ingredient type | MCSG-1 |
---|---|
Buffer | 96 |
Cryoprotectant | 6 |
Organic (non-volatile) | 11 |
Organic (volatile) | 1 |
Polymer | 78 |
Salt | 99 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 7.5 | 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
A2 |
| pH 9.5 | 100 μl of CHES pH 9.5 (1 M) 600 μl of Polyethylene glycol 3,000 (50 w/v) 300 μl of water (100 v/v) |
A3 |
| pH 6.2 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40 μl of Sodium chloride (5 M) 726 μl of water (100 v/v) |
A4 |
| pH 7 | 33 μl of tris chloride BCC pH 7 (3 M) 100 μl of Magnesium chloride (2 M) 500 μl of Sodium chloride (5 M) 366 μl of water (100 v/v) |
A5 |
| pH 4.5 | 360 μl of Ammonium sulfate (3.5 M) 50 μl of Sodium acetate pH 4.5 (2 M) 40 μl of Sodium chloride (5 M) 550 μl of water (100 v/v) |
A6 |
| pH 5.5 | 57 μl of Ammonium sulfate (3.5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 342 μl of water (100 v/v) |
A7 |
| pH 5.5 | 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of water (100 v/v) |
A8 |
| pH 7 | 33 μl of tris chloride BCC pH 7 (3 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 566 μl of water (100 v/v) |
A9 |
| pH 7.5 | 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |
A10 |
| pH 7.5 | 100 μl of Calcium chloride (2 M) 280 μl of Polypropylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 520 μl of water (100 v/v) |
A11 |
| pH 6.5 | 100 μl of Magnesium chloride (2 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 600 μl of water (100 v/v) |
A12 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Calcium chloride (2 M) 400 μl of water (100 v/v) |
B1 |
| pH 6.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 120 μl of Sodium chloride (5 M) 380 μl of water (100 v/v) |
B2 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
B3 |
| pH 5.5 | 200 μl of Ammonium acetate (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of water (100 v/v) |
B4 |
| pH 6.5 | 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 300 μl of water (100 v/v) |
B5 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 300 μl of water (100 v/v) |
B6 |
| pH 6 | 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Calcium acetate (1 M) 33 μl of MES pH 6 (3 M) 366 μl of water (100 v/v) |
B7 |
| pH 5.6 | 85 μl of tri-Sodium citrate pH 5.6 (1 M) 150 μl of Glycerol (100 v/v) 510 μl of Polyethylene glycol 4,000 (50 w/v) 170 μl of Ammonium acetate (1 M) 85 μl of water (100 v/v) |
B8 |
| pH 8.5 | 85 μl of tris chloride BCC pH 8.5 (1 M) 150 μl of Glycerol (100 v/v) 510 μl of Polyethylene glycol 4,000 (50 w/v) 56 μl of Sodium acetate (3 M) 198 μl of water (100 v/v) |
B9 |
| NA | 100 μl of Magnesium chloride (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of water (100 v/v) |
B10 |
| pH 8.5 | 80 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Glycerol (100 v/v) 80 μl of Magnesium chloride (2 M) 480 μl of Polyethylene glycol 4,000 (50 w/v) 160 μl of water (100 v/v) |
B11 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Magnesium chloride (2 M) 400 μl of water (100 v/v) |
B12 |
| pH 6.5 | 560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 340 μl of water (100 v/v) |
C1 |
| pH 5.5 | 100 μl of Ammonium acetate (1 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 460 μl of water (100 v/v) |
C2 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Lithium sulfate (2 M) 300 μl of water (100 v/v) |
C3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium formate pH 5.9 (1 M) 400 μl of water (100 v/v) |
C4 |
| pH 4.6 | 150 μl of Glycerol (100 v/v) 510 μl of Polyethylene glycol 4,000 (50 w/v) 170 μl of Ammonium acetate (1 M) 85 μl of Sodium acetate pH 4.6 (1 M) 85 μl of water (100 v/v) |
C5 |
| NA | 200 μl of Magnesium acetate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
C6 |
| pH 7.5 | 100 μl of Magnesium chloride (2 M) 300 μl of Polypropylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
C7 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Calcium chloride (2 M) 300 μl of water (100 v/v) |
C8 |
| pH 5.6 | 57 μl of Ammonium sulfate (3.5 M) 100 μl of tri-Sodium citrate pH 5.6 (1 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 342 μl of water (100 v/v) |
C9 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Lithium chloride (10 M) 640 μl of Polyethylene glycol 4,000 (50 w/v) 180 μl of water (100 v/v) |
C10 |
| NA | 600 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Lithium chloride (10 M) 33 μl of Sodium acetate (3 M) 266 μl of water (100 v/v) |
C11 |
| pH 7 | 33 μl of tris chloride BCC pH 7 (3 M) 200 μl of Calcium acetate (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 366 μl of water (100 v/v) |
C12 |
| pH 6.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 400 μl of water (100 v/v) |
D1 |
| pH 6.5 | 57 μl of Ammonium sulfate (3.5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 342 μl of water (100 v/v) |
D2 |
| pH 6.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
D3 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 300 μl of Polyethylene glycol 400 (100 v/v) 500 μl of water (100 v/v) |
D4 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 25 μl of Sodium thiocyanate pH 6.9 (8 M) 575 μl of water (100 v/v) |
D5 |
| NA | 20 μl of Ammonium formate pH 6.6 (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v) |
D6 |
| pH 7 | 411 μl of di-Sodium malonate pH 7 (3.4 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 488 μl of water (100 v/v) |
D7 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 500 μl of water (100 v/v) |
D8 |
| pH 6.5 | 428 μl of Ammonium sulfate (3.5 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 20 μl of Sodium chloride (5 M) 451 μl of water (100 v/v) |
D9 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Sodium chloride (5 M) 360 μl of water (100 v/v) |
D10 |
| pH 6.5 | 200 μl of Calcium acetate (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of water (100 v/v) |
D11 |
| NA | 200 μl of Sodium iodide (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
D12 |
| NA | 200 μl of Ammonium chloride pH 6.3 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
E1 |
| pH 7.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of HEPES pH 7.5 (1 M) 328 μl of water (100 v/v) |
E2 |
| pH 6.5 | 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 25 μl of Calcium chloride (2 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 575 μl of water (100 v/v) |
E3 |
| pH 7.5 | 25 μl of Magnesium chloride (2 M) 300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 575 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Lithium sulfate (2 M) 300 μl of water (100 v/v) |
E5 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 800 μl of Magnesium sulfate (2 M) 100 μl of water (100 v/v) |
E6 |
| NA | 400 μl of Potassium sulfate (0.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of water (100 v/v) |
E7 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Ammonium iodide (2 M) 500 μl of water (100 v/v) |
E8 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 900 μl of Magnesium sulfate (2 M) |
E9 |
| NA | 100 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of water (100 v/v) |
E10 |
| NA | 100 μl of di-Ammonium tartrate (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 500 μl of water (100 v/v) |
E11 |
| pH 8.2 | 333 μl of Sodium Potassium phosphate pH 8.2 (3 M) 666 μl of water (100 v/v) |
E12 |
| NA | 705 μl of di-Sodium malonate pH 7 (3.4 M) 294 μl of water (100 v/v) |
F1 |
| pH 6.5 | 400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 500 μl of water (100 v/v) |
F2 |
| pH 6.5 | 200 μl of Ammonium acetate (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 200 μl of water (100 v/v) |
F3 |
| NA | 80 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v) |
F4 |
| pH 5.5 | 100 μl of tri-Sodium citrate pH 5.5 (1 M) 100 μl of Magnesium chloride (2 M) 400 μl of Polyethylene glycol 400 (100 v/v) 400 μl of water (100 v/v) |
F5 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of Sodium acetate (3 M) 533 μl of water (100 v/v) |
F6 |
| NA | 25 μl of Potassium thiocyanate (8 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 575 μl of water (100 v/v) |
F7 |
| pH 5.6 | 186 μl of Sodium Potassium phosphate pH 8.8 (3 M) 146 μl of Sodium Potassium phosphate pH 4.6 (3 M) 666 μl of water (100 v/v) |
F8 |
| NA | 500 μl of Sodium formate pH 7 (7 M) 500 μl of water (100 v/v) |
F9 |
| pH 6.5 | 14 μl of Ammonium sulfate (3.5 M) 600 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v) 50 μl of BIS-TRIS pH 6.5 (1 M) 335 μl of water (100 v/v) |
F10 |
| pH 8.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 328 μl of water (100 v/v) |
F11 |
| pH 7.5 | 57 μl of Ammonium sulfate (3.5 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 342 μl of water (100 v/v) |
F12 |
| NA | 12 μl of Potassium thiocyanate (8 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 387 μl of water (100 v/v) |
G1 |
| pH 7.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Ethylene glycol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v) |
G2 |
| NA | 320 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 26 μl of Potassium di-hydrogen phosphate (1.5 M) 453 μl of water (100 v/v) |
G3 |
| pH 7 | 428 μl of Ammonium sulfate (3.5 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 471 μl of water (100 v/v) |
G4 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 133 μl of Potassium Sodium tartrate (1.5 M) 466 μl of water (100 v/v) |
G5 |
| pH 6.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of BIS-TRIS pH 6.5 (1 M) 328 μl of water (100 v/v) |
G6 |
| pH 4.5 | 50 μl of Sodium acetate pH 4.5 (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 450 μl of water (100 v/v) |
G7 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
G8 |
| pH 8.5 | 57 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 342 μl of water (100 v/v) |
G9 |
| NA | 28 μl of Sodium formate (7 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 571 μl of water (100 v/v) |
G10 |
| NA | 100 μl of Magnesium formate (1 M) 300 μl of Polyethylene glycol 3,350 (50 w/v) 600 μl of water (100 v/v) |
G11 |
| pH 4.2 | 400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 500 μl of water (100 v/v) |
G12 |
| pH 6.5 | 288 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 160 μl of Calcium acetate (1 M) 80 μl of Sodium cacodylate pH 6.5 (1 M) 272 μl of water (100 v/v) |
H1 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium fluoride (10 M) 580 μl of water (100 v/v) |
H2 |
| NA | 200 μl of Potassium iodide (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
H3 |
| NA | 40 μl of Lithium acetate (5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 560 μl of water (100 v/v) |
H4 |
| pH 5.6 | 100 μl of tri-Sodium citrate pH 5.6 (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 300 μl of water (100 v/v) |
H5 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of Potassium chloride (4 M) 550 μl of water (100 v/v) |
H6 |
| pH 4.6 | 500 μl of Sodium formate (7 M) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of water (100 v/v) |
H7 |
| pH 5.5 | 571 μl of Ammonium sulfate (3.5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 328 μl of water (100 v/v) |
H8 |
| pH 7 | 20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 833 μl of di-Sodium succinate pH 7 (1.2 M) 100 μl of HEPES pH 7 (1 M) 46 μl of water (100 v/v) |
H9 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 400 μl of water (100 v/v) |
H10 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v) |
H11 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Sodium chloride (5 M) 100 μl of HEPES pH 7.5 (1 M) 360 μl of water (100 v/v) |
H12 |
| pH 7.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Lithium sulfate (2 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v) |