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Supplemental Information

Screen Description

MCSG-1

Created 2011-06-15.

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MCSG-1

    Screen Structure

    MCSG-1

    Well Count 96
    Number of distinct ingredients 57
    Internal diversity (score mean dist) 0.911

    Screen Composition

    2025-06-14T13:45:41.218421 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MCSG-1
    Buffer 96
    Cryoprotectant 6
    Organic (non-volatile) 11
    Organic (volatile) 1
    Polymer 78
    Salt 99

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MCSG-1 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 8,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    A2
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 3,000 30 w/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    300 μl of water (100 v/v)
    A3
    • Sodium chloride 0.2 M -
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 8,000 10 w/v -
    pH 6.2
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    40 μl of Sodium chloride (5 M)
    726 μl of water (100 v/v)
    A4
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Sodium chloride 2.5 M -
    pH 7
    33 μl of tris chloride BCC pH 7 (3 M)
    100 μl of Magnesium chloride (2 M)
    500 μl of Sodium chloride (5 M)
    366 μl of water (100 v/v)
    A5
    • Sodium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 1.26 M -
    pH 4.5
    360 μl of Ammonium sulfate (3.5 M)
    50 μl of Sodium acetate pH 4.5 (2 M)
    40 μl of Sodium chloride (5 M)
    550 μl of water (100 v/v)
    A6
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    342 μl of water (100 v/v)
    A7
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of Magnesium chloride (2 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    300 μl of water (100 v/v)
    A8
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    33 μl of tris chloride BCC pH 7 (3 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    566 μl of water (100 v/v)
    A9
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    100 μl of Magnesium chloride (2 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    A10
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polypropylene glycol 400 28 v/v -
    pH 7.5
    100 μl of Calcium chloride (2 M)
    280 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    A11
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 6.5
    100 μl of Magnesium chloride (2 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    A12
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Calcium chloride (2 M)
    400 μl of water (100 v/v)
    B1
    • Sodium chloride 0.6 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    120 μl of Sodium chloride (5 M)
    380 μl of water (100 v/v)
    B2
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    B3
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of water (100 v/v)
    B4
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of Magnesium chloride (2 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    300 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    300 μl of water (100 v/v)
    B6
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 8,000 20 w/v -
    pH 6
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Calcium acetate (1 M)
    33 μl of MES pH 6 (3 M)
    366 μl of water (100 v/v)
    B7
    • Ammonium acetate 0.17 M -
    • tri-Sodium citrate 0.085 M pH 5.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 5.6
    85 μl of tri-Sodium citrate pH 5.6 (1 M)
    150 μl of Glycerol (100 v/v)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    170 μl of Ammonium acetate (1 M)
    85 μl of water (100 v/v)
    B8
    • Sodium acetate 0.17 M -
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 8.5
    85 μl of tris chloride BCC pH 8.5 (1 M)
    150 μl of Glycerol (100 v/v)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    56 μl of Sodium acetate (3 M)
    198 μl of water (100 v/v)
    B9
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    100 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    B10
    • Magnesium chloride 0.16 M -
    • tris chloride BCC 0.08 M pH 8.5
    • Polyethylene glycol 4,000 24 w/v -
    • Glycerol 20 v/v -
    pH 8.5
    80 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Glycerol (100 v/v)
    80 μl of Magnesium chloride (2 M)
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    160 μl of water (100 v/v)
    B11
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    400 μl of water (100 v/v)
    B12
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    pH 6.5
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    340 μl of water (100 v/v)
    C1
    • Ammonium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 10,000 17 w/v -
    pH 5.5
    100 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    460 μl of water (100 v/v)
    C2
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    C3
    • Magnesium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium formate pH 5.9 (1 M)
    400 μl of water (100 v/v)
    C4
    • Ammonium acetate 0.17 M -
    • Sodium acetate 0.085 M pH 4.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    pH 4.6
    150 μl of Glycerol (100 v/v)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    170 μl of Ammonium acetate (1 M)
    85 μl of Sodium acetate pH 4.6 (1 M)
    85 μl of water (100 v/v)
    C5
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    200 μl of Magnesium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    C6
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polypropylene glycol 400 30 v/v -
    pH 7.5
    100 μl of Magnesium chloride (2 M)
    300 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    C7
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Calcium chloride (2 M)
    300 μl of water (100 v/v)
    C8
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 25 w/v -
    pH 5.6
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    342 μl of water (100 v/v)
    C9
    • Lithium chloride 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 32 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Lithium chloride (10 M)
    640 μl of Polyethylene glycol 4,000 (50 w/v)
    180 μl of water (100 v/v)
    C10
    • Lithium chloride 1 M -
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 6,000 30 w/v -
    NA
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of Lithium chloride (10 M)
    33 μl of Sodium acetate (3 M)
    266 μl of water (100 v/v)
    C11
    • Calcium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,000 20 w/v -
    pH 7
    33 μl of tris chloride BCC pH 7 (3 M)
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    366 μl of water (100 v/v)
    C12
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of water (100 v/v)
    D1
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    342 μl of water (100 v/v)
    D2
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium chloride 0.2 M -
    pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    D3
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    D4
    • Sodium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate pH 6.9 (8 M)
    575 μl of water (100 v/v)
    D5
    • Ammonium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    20 μl of Ammonium formate pH 6.6 (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    D6
    • di-Sodium malonate 1.4 M -
    • BIS-TRIS propane 0.1 M pH 7
    pH 7
    411 μl of di-Sodium malonate pH 7 (3.4 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    488 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,000 20 w/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    500 μl of water (100 v/v)
    D8
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 1.5 M -
    pH 6.5
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    451 μl of water (100 v/v)
    D9
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    D10
    • Calcium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 300 40 v/v -
    pH 6.5
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of water (100 v/v)
    D11
    • Sodium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    200 μl of Sodium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D12
    • Ammonium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    200 μl of Ammonium chloride pH 6.3 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    E1
    • Ammonium sulfate 2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    328 μl of water (100 v/v)
    E2
    • Calcium chloride 0.05 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    25 μl of Calcium chloride (2 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    575 μl of water (100 v/v)
    E3
    • Magnesium chloride 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 7.5
    25 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    575 μl of water (100 v/v)
    E4
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    E5
    • Magnesium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    800 μl of Magnesium sulfate (2 M)
    100 μl of water (100 v/v)
    E6
    • Potassium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Potassium sulfate (0.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    E7
    • Ammonium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Ammonium iodide (2 M)
    500 μl of water (100 v/v)
    E8
    • Magnesium sulfate 1.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    900 μl of Magnesium sulfate (2 M)
    E9
    • Calcium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    100 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E10
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    100 μl of di-Ammonium tartrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E11
    • Sodium Potassium phosphate 1 M pH 8.2
    pH 8.2
    333 μl of Sodium Potassium phosphate pH 8.2 (3 M)
    666 μl of water (100 v/v)
    E12
    • di-Sodium malonate 2.4 M -
    NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    F1
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 6.5
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of water (100 v/v)
    F2
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of water (100 v/v)
    F3
    • di-Ammonium hydrogen citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    80 μl of di-Ammonium hydrogen citrate pH 5 (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    F4
    • Magnesium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    F5
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Sodium acetate (3 M)
    533 μl of water (100 v/v)
    F6
    • Potassium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    25 μl of Potassium thiocyanate (8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    575 μl of water (100 v/v)
    F7
    • Sodium Potassium phosphate 1 M pH 5.6
    pH 5.6
    186 μl of Sodium Potassium phosphate pH 8.8 (3 M)
    146 μl of Sodium Potassium phosphate pH 4.6 (3 M)
    666 μl of water (100 v/v)
    F8
    • Sodium formate 3.5 M -
    NA
    500 μl of Sodium formate pH 7 (7 M)
    500 μl of water (100 v/v)
    F9
    • Ammonium sulfate 0.05 M -
    • BIS-TRIS 0.05 M pH 6.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
    pH 6.5
    14 μl of Ammonium sulfate (3.5 M)
    600 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    50 μl of BIS-TRIS pH 6.5 (1 M)
    335 μl of water (100 v/v)
    F10
    • Ammonium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    F11
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    342 μl of water (100 v/v)
    F12
    • Potassium thiocyanate 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    NA
    12 μl of Potassium thiocyanate (8 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    387 μl of water (100 v/v)
    G1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 10 w/v -
    • Ethylene glycol 8 v/v -
    pH 7.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    G2
    • Potassium di-hydrogen phosphate 0.04 M -
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    NA
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    26 μl of Potassium di-hydrogen phosphate (1.5 M)
    453 μl of water (100 v/v)
    G3
    • BIS-TRIS propane 0.1 M pH 7
    • Ammonium sulfate 1.5 M -
    pH 7
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    471 μl of water (100 v/v)
    G4
    • Potassium Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of Potassium Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    G5
    • Ammonium sulfate 2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    328 μl of water (100 v/v)
    G6
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (2 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    450 μl of water (100 v/v)
    G7
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    G8
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    342 μl of water (100 v/v)
    G9
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium formate 0.2 M -
    NA
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    G10
    • Polyethylene glycol 3,350 15 w/v -
    • Magnesium formate 0.1 M -
    NA
    100 μl of Magnesium formate (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    G11
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    pH 4.2
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    500 μl of water (100 v/v)
    G12
    • Calcium acetate 0.16 M -
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 14.4 w/v -
    • Glycerol 20 v/v -
    pH 6.5
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    160 μl of Calcium acetate (1 M)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    272 μl of water (100 v/v)
    H1
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium fluoride 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium fluoride (10 M)
    580 μl of water (100 v/v)
    H2
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium iodide 0.2 M -
    NA
    200 μl of Potassium iodide (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    H3
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium acetate 0.2 M -
    NA
    40 μl of Lithium acetate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    H4
    • tri-Sodium citrate 0.1 M pH 5.6
    • 2-Propanol 20 v/v -
    • Polyethylene glycol 4,000 20 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    H5
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium chloride 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Potassium chloride (4 M)
    550 μl of water (100 v/v)
    H6
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 3.5 M -
    pH 4.6
    500 μl of Sodium formate (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of water (100 v/v)
    H7
    • Ammonium sulfate 2 M -
    • BIS-TRIS 0.1 M pH 5.5
    pH 5.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    328 μl of water (100 v/v)
    H8
    • di-Sodium succinate 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 1 w/v -
    pH 7
    20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    833 μl of di-Sodium succinate pH 7 (1.2 M)
    100 μl of HEPES pH 7 (1 M)
    46 μl of water (100 v/v)
    H9
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of water (100 v/v)
    H10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    H11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium chloride 0.2 M -
    pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    360 μl of water (100 v/v)
    H12
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    • Lithium sulfate 0.2 M -
    pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)