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Supplemental Information

Screen Description

MCSG-2

Created 2011-06-15.

Export

Barcodes

MCSG-2

No barcodes found.

Screen Structure

MCSG-2

Well Count 96
Number of distinct ingredients 71
Internal diversity (score mean dist) 0.956

Screen Composition

2025-08-16T14:28:00.071211 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MCSG-2
Buffer 104
Complex ingredient 2
Cryoprotectant 1
Organic (non-volatile) 8
Organic (volatile) 4
Polymer 53
Salt 115

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MCSG-2 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
A2
  • Potassium bromide 0.15 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
A3
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 8,000 20 w/v -
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.5
  • Sodium di-hydrogen phosphate 0.8 M -
  • di-Potassium hydrogen phosphate 1.2 M -
pH 4.5
50.0 μl of Sodium acetate pH 4.5 (2 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
300.0 μl of di-Potassium hydrogen phosphate (4 M)
450.0 μl of water (100 v/v)
A5
  • Lithium sulfate 0.2 M -
  • CAPS 0.1 M pH 10
  • Sodium di-hydrogen phosphate 1.2 M -
  • di-Potassium hydrogen phosphate 0.8 M -
pH 10
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Sodium di-hydrogen phosphate (3 M)
200.0 μl of di-Potassium hydrogen phosphate (4 M)
200.0 μl of water (100 v/v)
A6
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 1.26 M -
pH 6.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
540.0 μl of water (100 v/v)
A7
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 3,000 10 w/v -
pH 4.2
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
660.0 μl of water (100 v/v)
A8
  • Potassium formate 0.2 M pH 7.3
  • Polyethylene glycol 3,350 20 w/v -
pH 7.3
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium formate pH 7.3 (1 M)
400.0 μl of water (100 v/v)
A9
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
A10
  • Ammonium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium nitrate pH 6.3 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A11
  • Lithium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A12
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 600 40 w/v -
pH 5.5
800.0 μl of Polyethylene glycol 600 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
100.0 μl of water (100 v/v)
B1
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
B2
  • Potassium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Potassium acetate (5 M)
560.0 μl of water (100 v/v)
B3
  • di-Ammonium hydrogen citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
520.0 μl of water (100 v/v)
B4
  • Sodium Potassium phosphate 1.8 M pH 8.2
pH 8.2
600.0 μl of Sodium Potassium phosphate pH 8.2 (3 M)
400.0 μl of water (100 v/v)
B5
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
242.9 μl of water (100 v/v)
B6
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
B7
  • BIS-TRIS propane 0.1 M pH 7
  • Sodium acetate 2.8 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
700.0 μl of Sodium acetate (4 M)
200.0 μl of water (100 v/v)
B8
  • Tacsimate 60 v/v -
NA
600.0 μl of Tacsimate pH 7 (100 v/v)
400.0 μl of water (100 v/v)
B9
  • Ammonium sulfate 1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 1 w/v -
pH 5.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
594.3 μl of water (100 v/v)
B10
  • di-Sodium malonate 1.1 M -
  • HEPES 0.1 M pH 7
  • Jeffamine® ED-2001 0.5 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
323.5 μl of di-Sodium malonate pH 7 (3.4 M)
566.5 μl of water (100 v/v)
B11
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
B12
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
C1
  • di-Sodium succinate 0.8 M -
NA
666.7 μl of di-Sodium succinate pH 7 (1.2 M)
333.3 μl of water (100 v/v)
C2
  • Tacsimate 35 v/v -
NA
350.0 μl of Tacsimate pH 7 (100 v/v)
650.0 μl of water (100 v/v)
C3
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
C4
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of tri-Ammonium citrate pH 7 (3 M)
533.3 μl of water (100 v/v)
C5
  • tri-Sodium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate pH 7 (1.6 M)
475.0 μl of water (100 v/v)
C6
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 6,000 20 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C7
  • di-Ammonium hydrogen phosphate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
C8
  • Magnesium sulfate 1.8 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
900.0 μl of Magnesium sulfate (2 M)
C9
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
C10
  • Sodium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium nitrate (7 M)
571.4 μl of water (100 v/v)
C11
  • di-Ammonium tartrate 1.3 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
650.0 μl of di-Ammonium tartrate (2 M)
250.0 μl of water (100 v/v)
C12
  • di-Ammonium tartrate 1.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
700.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
D1
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
471.4 μl of water (100 v/v)
D2
  • Ammonium sulfate 1.26 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
440.0 μl of water (100 v/v)
D3
  • Ammonium sulfate 2 M -
  • CAPS 0.1 M pH 10
  • Lithium sulfate 0.2 M -
pH 10
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Lithium sulfate (2 M)
228.6 μl of water (100 v/v)
D4
  • Calcium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Sodium chloride 3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D6
  • Sodium acetate 2.8 M -
NA
700.0 μl of Sodium acetate pH 7 (4 M)
300.0 μl of water (100 v/v)
D7
  • di-Ammonium tartrate 1.1 M -
NA
550.0 μl of di-Ammonium tartrate pH 7 (2 M)
450.0 μl of water (100 v/v)
D8
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate pH 6.9 (3 M)
533.3 μl of water (100 v/v)
D9
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
400.0 μl of water (100 v/v)
D10
  • tri-Lithium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Lithium citrate (1.5 M)
466.7 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 1.5 M -
pH 8.5
750.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
D12
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
542.9 μl of water (100 v/v)
E1
  • BIS-TRIS propane 0.1 M pH 7
  • Sodium acetate 1.8 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
450.0 μl of Sodium acetate pH 7 (4 M)
450.0 μl of water (100 v/v)
E2
  • BIS-TRIS propane 0.1 M pH 7
  • Sodium chloride 3.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
640.0 μl of Sodium chloride (5 M)
260.0 μl of water (100 v/v)
E3
  • BIS-TRIS propane 0.1 M pH 7
  • Lithium sulfate 1.5 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
  • 1,4-Dioxane 10 w/v -
pH 6.5
200.0 μl of 1,4-Dioxane (50 w/v)
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
242.9 μl of water (100 v/v)
E5
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 8,000 30 w/v -
pH 4.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.3 μl of Sodium acetate pH 4.5 (3 M)
266.7 μl of water (100 v/v)
E6
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
33.3 μl of Sodium acetate pH 4.5 (3 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
681.0 μl of water (100 v/v)
E7
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 20 w/v -
pH 4.2
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
E8
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
E9
  • CHES 0.1 M pH 9.5
  • tri-Sodium citrate 1 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
625.0 μl of tri-Sodium citrate pH 7 (1.6 M)
275.0 μl of water (100 v/v)
E10
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Sodium di-hydrogen phosphate 1.6 M -
  • di-Potassium hydrogen phosphate 0.4 M -
pH 4.2
400.0 μl of Sodium di-hydrogen phosphate (4 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
100.0 μl of di-Potassium hydrogen phosphate (4 M)
400.0 μl of water (100 v/v)
E11
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 3,000 10 w/v -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
E12
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
F1
  • Sodium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
  • di-Ammonium hydrogen phosphate 1 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
574.3 μl of water (100 v/v)
F2
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
F3
  • Sodium chloride 3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
F4
  • Sodium Potassium phosphate 1 M -
NA
1000.0 μl of Sodium Potassium phosphate pH 7 (1 M)
F5
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
  • Poly(acrylic acid sodium salt) 5,100 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Magnesium chloride (2 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
450.0 μl of water (100 v/v)
F6
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
541.2 μl of water (100 v/v)
F7
  • di-Sodium DL-malate 0.15 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
550.0 μl of water (100 v/v)
F8
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
F9
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 10 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
F11
  • di-Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of di-Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
F12
  • Lithium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
25.0 μl of Lithium nitrate (8 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
575.0 μl of water (100 v/v)
G1
  • di-Sodium hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of di-Sodium hydrogen phosphate (1 M)
400.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.1 M pH 8.5
  • di-Ammonium hydrogen phosphate 2.4 M -
pH 8.5
685.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
214.3 μl of water (100 v/v)
G3
  • Sodium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
  • di-Potassium hydrogen phosphate 0.4 M -
  • Sodium di-hydrogen phosphate 1.6 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
400.0 μl of Sodium di-hydrogen phosphate (4 M)
100.0 μl of di-Potassium hydrogen phosphate (4 M)
360.0 μl of water (100 v/v)
G4
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
  • 2-Propanol 10 v/v -
pH 6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Calcium acetate (1 M)
33.3 μl of MES pH 6 (3 M)
666.7 μl of water (100 v/v)
G5
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
  • Ammonium sulfate 1.26 M -
pH 9.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of CHES pH 9.5 (1 M)
40.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
G6
  • Sodium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • di-Ammonium hydrogen phosphate 1 M -
pH 5.5
40.0 μl of Sodium chloride (5 M)
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
574.3 μl of water (100 v/v)
G7
  • tris chloride BCC 0.1 M pH 7
  • Ethanol 15 v/v -
pH 7
150.0 μl of Ethanol (100 v/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
816.7 μl of water (100 v/v)
G8
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 1,000 20 w/v -
pH 7
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.3 μl of tris chloride BCC pH 7 (3 M)
566.7 μl of water (100 v/v)
G9
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 1,000 20 w/v -
  • Sodium chloride 0.2 M -
pH 6.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
40.0 μl of Sodium chloride (5 M)
526.7 μl of water (100 v/v)
G10
  • CAPS 0.1 M pH 10
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
pH 10
100.0 μl of CAPS pH 10 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7
  • Jeffamine® ED-2001 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
G12
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H1
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H2
  • Potassium chloride 0.2 M -
  • HEPES 0.05 M pH 7.5
  • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
50.0 μl of Potassium chloride (4 M)
200.0 μl of water (100 v/v)
H3
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H4
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
200.0 μl of Ammonium acetate (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
H5
  • di-Sodium succinate 0.1 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
83.3 μl of di-Sodium succinate pH 7 (1.2 M)
616.7 μl of water (100 v/v)
H6
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
671.4 μl of water (100 v/v)
H7
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 20,000 10 w/v -
  • 1,4-Dioxane 2 v/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
H8
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
H9
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2.5 M -
pH 4.6
833.3 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
66.7 μl of water (100 v/v)
H10
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
H11
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2.5 M -
pH 8.5
833.3 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
66.7 μl of water (100 v/v)
H12
  • Zinc acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,000 10 w/v -
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
33.3 μl of Sodium acetate pH 4.5 (3 M)
100.0 μl of Zinc acetate (2 M)
666.7 μl of water (100 v/v)