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Supplemental Information

Screen Description

MCSG-2

Created 2011-06-15.

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MCSG-2

    Screen Structure

    MCSG-2

    Well Count 96
    Number of distinct ingredients 71
    Internal diversity (score mean dist) 0.956

    Screen Composition

    2025-06-14T14:01:14.428895 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MCSG-2
    Buffer 104
    Complex ingredient 2
    Cryoprotectant 1
    Organic (non-volatile) 8
    Organic (volatile) 4
    Polymer 53
    Salt 115

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MCSG-2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Potassium Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of Potassium Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    A2
    • Potassium bromide 0.15 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    37 μl of Potassium bromide (4 M)
    362 μl of water (100 v/v)
    A3
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 8,000 20 w/v -
    pH 4.2
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Sodium di-hydrogen phosphate 0.8 M -
    • di-Potassium hydrogen phosphate 1.2 M -
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (2 M)
    300 μl of di-Potassium hydrogen phosphate (4 M)
    200 μl of Sodium di-hydrogen phosphate (4 M)
    450 μl of water (100 v/v)
    A5
    • Lithium sulfate 0.2 M -
    • CAPS 0.1 M pH 10
    • Sodium di-hydrogen phosphate 1.2 M -
    • di-Potassium hydrogen phosphate 0.8 M -
    pH 10
    200 μl of di-Potassium hydrogen phosphate (4 M)
    100 μl of CAPS pH 10 (1 M)
    400 μl of Sodium di-hydrogen phosphate (3 M)
    100 μl of Lithium sulfate (2 M)
    200 μl of water (100 v/v)
    A6
    • Sodium cacodylate 0.1 M pH 6.5
    • Ammonium sulfate 1.26 M -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    360 μl of Ammonium sulfate (3.5 M)
    540 μl of water (100 v/v)
    A7
    • Sodium chloride 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 3,000 10 w/v -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    40 μl of Sodium chloride (5 M)
    660 μl of water (100 v/v)
    A8
    • Potassium formate 0.2 M pH 7.3
    • Polyethylene glycol 3,350 20 w/v -
    pH 7.3
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Potassium formate pH 7.3 (1 M)
    400 μl of water (100 v/v)
    A9
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    A10
    • Ammonium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium nitrate pH 6.3 (10 M)
    580 μl of water (100 v/v)
    A11
    • Lithium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Lithium chloride (10 M)
    580 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 600 40 w/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    800 μl of Polyethylene glycol 600 (50 w/v)
    100 μl of water (100 v/v)
    B1
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    pH 6.5
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    B2
    • Potassium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    40 μl of Potassium acetate (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    B3
    • di-Ammonium hydrogen citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    B4
    • Sodium Potassium phosphate 1.8 M pH 8.2
    pH 8.2
    600 μl of Sodium Potassium phosphate pH 8.2 (3 M)
    400 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    242 μl of water (100 v/v)
    B6
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    28 μl of Sodium formate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    B7
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium acetate 2.8 M -
    pH 7
    700 μl of Sodium acetate (4 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    200 μl of water (100 v/v)
    B8
    • Tacsimate 60 v/v -
    NA
    600 μl of Tacsimate pH 7 (100 v/v)
    400 μl of water (100 v/v)
    B9
    • Ammonium sulfate 1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 1 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    285 μl of Ammonium sulfate (3.5 M)
    594 μl of water (100 v/v)
    B10
    • di-Sodium malonate 1.1 M -
    • HEPES 0.1 M pH 7
    • Jeffamine® ED-2001 0.5 v/v -
    pH 7
    10 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    323 μl of di-Sodium malonate pH 7 (3.4 M)
    566 μl of water (100 v/v)
    B11
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    B12
    • Sodium chloride 3 M -
    • BIS-TRIS 0.1 M pH 5.5
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    C1
    • di-Sodium succinate 0.8 M -
    NA
    666 μl of di-Sodium succinate pH 7 (1.2 M)
    333 μl of water (100 v/v)
    C2
    • Tacsimate 35 v/v -
    NA
    350 μl of Tacsimate pH 7 (100 v/v)
    650 μl of water (100 v/v)
    C3
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of water (100 v/v)
    C4
    • tri-Ammonium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of tri-Ammonium citrate pH 7 (3 M)
    533 μl of water (100 v/v)
    C5
    • tri-Sodium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    125 μl of tri-Sodium citrate pH 7 (1.6 M)
    475 μl of water (100 v/v)
    C6
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 6,000 20 w/v -
    pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    C7
    • di-Ammonium hydrogen phosphate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    428 μl of di-Ammonium hydrogen phosphate (3.5 M)
    471 μl of water (100 v/v)
    C8
    • Magnesium sulfate 1.8 M -
    • BIS-TRIS propane 0.1 M pH 7
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    900 μl of Magnesium sulfate (2 M)
    C9
    • Sodium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    250 μl of Sodium fluoride (0.8 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    350 μl of water (100 v/v)
    C10
    • Sodium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium nitrate (7 M)
    571 μl of water (100 v/v)
    C11
    • di-Ammonium tartrate 1.3 M -
    • BIS-TRIS propane 0.1 M pH 7
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    650 μl of di-Ammonium tartrate (2 M)
    250 μl of water (100 v/v)
    C12
    • di-Ammonium tartrate 1.4 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of di-Ammonium tartrate (2 M)
    200 μl of water (100 v/v)
    D1
    • Ammonium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    471 μl of water (100 v/v)
    D2
    • Ammonium sulfate 1.26 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    360 μl of Ammonium sulfate (3.5 M)
    100 μl of Lithium sulfate (2 M)
    440 μl of water (100 v/v)
    D3
    • Ammonium sulfate 2 M -
    • CAPS 0.1 M pH 10
    • Lithium sulfate 0.2 M -
    pH 10
    100 μl of CAPS pH 10 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Lithium sulfate (2 M)
    228 μl of water (100 v/v)
    D4
    • Calcium acetate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 4.5
    • Polyethylene glycol 400 30 v/v -
    pH 4.5
    100 μl of tri-Sodium citrate pH 4.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    200 μl of Calcium acetate (1 M)
    400 μl of water (100 v/v)
    D5
    • Sodium chloride 3 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    D6
    • Sodium acetate 2.8 M -
    NA
    700 μl of Sodium acetate pH 7 (4 M)
    300 μl of water (100 v/v)
    D7
    • di-Ammonium tartrate 1.1 M -
    NA
    550 μl of di-Ammonium tartrate pH 7 (2 M)
    450 μl of water (100 v/v)
    D8
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    66 μl of Potassium nitrate pH 6.9 (3 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    533 μl of water (100 v/v)
    D9
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    100 μl of Lithium chloride (10 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    400 μl of water (100 v/v)
    D10
    • tri-Lithium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    133 μl of tri-Lithium citrate (1.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    466 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 1.5 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    750 μl of Lithium sulfate (2 M)
    150 μl of water (100 v/v)
    D12
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    542 μl of water (100 v/v)
    E1
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium acetate 1.8 M -
    pH 7
    450 μl of Sodium acetate pH 7 (4 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    450 μl of water (100 v/v)
    E2
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium chloride 3.2 M -
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    640 μl of Sodium chloride (5 M)
    260 μl of water (100 v/v)
    E3
    • BIS-TRIS propane 0.1 M pH 7
    • Lithium sulfate 1.5 M -
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    750 μl of Lithium sulfate (2 M)
    150 μl of water (100 v/v)
    E4
    • Ammonium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    • 1,4-Dioxane 10 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    200 μl of 1,4-Dioxane (50 w/v)
    457 μl of Ammonium sulfate (3.5 M)
    242 μl of water (100 v/v)
    E5
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 8,000 30 w/v -
    pH 4.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    100 μl of Lithium sulfate (2 M)
    266 μl of water (100 v/v)
    E6
    • di-Ammonium hydrogen phosphate 1 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    33 μl of Sodium acetate pH 4.5 (3 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    681 μl of water (100 v/v)
    E7
    • Lithium sulfate 0.2 M -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 20 w/v -
    pH 4.2
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Lithium sulfate (2 M)
    400 μl of water (100 v/v)
    E8
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 20 w/v -
    pH 9.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CHES pH 9.5 (1 M)
    500 μl of water (100 v/v)
    E9
    • CHES 0.1 M pH 9.5
    • tri-Sodium citrate 1 M -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    625 μl of tri-Sodium citrate pH 7 (1.6 M)
    275 μl of water (100 v/v)
    E10
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Sodium di-hydrogen phosphate 1.6 M -
    • di-Potassium hydrogen phosphate 0.4 M -
    pH 4.2
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of di-Potassium hydrogen phosphate (4 M)
    400 μl of Sodium di-hydrogen phosphate (4 M)
    400 μl of water (100 v/v)
    E11
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 3,000 10 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    600 μl of water (100 v/v)
    E12
    • Lithium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    500 μl of water (100 v/v)
    F1
    • Sodium chloride 0.2 M -
    • Imidazole 0.1 M pH 8
    • di-Ammonium hydrogen phosphate 1 M -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    40 μl of Sodium chloride (5 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    574 μl of water (100 v/v)
    F2
    • Sodium chloride 3 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    600 μl of Sodium chloride (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F3
    • Sodium chloride 3 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    600 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    F4
    • Sodium Potassium phosphate 1 M -
    NA
    1000 μl of Sodium Potassium phosphate pH 7 (1 M)
    F5
    • Magnesium chloride 0.02 M -
    • HEPES 0.1 M pH 7.5
    • Poly(acrylic acid sodium salt) 5,100 22 w/v -
    pH 7.5
    440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    10 μl of Magnesium chloride (2 M)
    450 μl of water (100 v/v)
    F6
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    541 μl of water (100 v/v)
    F7
    • di-Sodium DL-malate 0.15 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of di-Sodium DL-malate pH 7 (3 M)
    550 μl of water (100 v/v)
    F8
    • Ammonium di-hydrogen phosphate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    pH 8.5
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    320 μl of water (100 v/v)
    F9
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 10 w/v -
    pH 7
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    700 μl of water (100 v/v)
    F10
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    NA
    200 μl of Glycerol (100 v/v)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    F11
    • di-Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of di-Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    F12
    • Lithium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Lithium nitrate (8 M)
    575 μl of water (100 v/v)
    G1
    • di-Sodium hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of di-Sodium hydrogen phosphate (1 M)
    400 μl of water (100 v/v)
    G2
    • tris chloride BCC 0.1 M pH 8.5
    • di-Ammonium hydrogen phosphate 2.4 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    685 μl of di-Ammonium hydrogen phosphate (3.5 M)
    214 μl of water (100 v/v)
    G3
    • Sodium chloride 0.2 M -
    • Imidazole 0.1 M pH 8
    • di-Potassium hydrogen phosphate 0.4 M -
    • Sodium di-hydrogen phosphate 1.6 M -
    pH 8
    100 μl of Imidazole pH 8 (1 M)
    100 μl of di-Potassium hydrogen phosphate (4 M)
    40 μl of Sodium chloride (5 M)
    400 μl of Sodium di-hydrogen phosphate (4 M)
    360 μl of water (100 v/v)
    G4
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6
    • 2-Propanol 10 v/v -
    pH 6
    33 μl of MES pH 6 (3 M)
    100 μl of 2-Propanol (100 v/v)
    200 μl of Calcium acetate (1 M)
    666 μl of water (100 v/v)
    G5
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Ammonium sulfate 1.26 M -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    40 μl of Sodium chloride (5 M)
    360 μl of Ammonium sulfate (3.5 M)
    500 μl of water (100 v/v)
    G6
    • Sodium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • di-Ammonium hydrogen phosphate 1 M -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    40 μl of Sodium chloride (5 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    574 μl of water (100 v/v)
    G7
    • tris chloride BCC 0.1 M pH 7
    • Ethanol 15 v/v -
    pH 7
    33 μl of tris chloride BCC pH 7 (3 M)
    150 μl of Ethanol (100 v/v)
    816 μl of water (100 v/v)
    G8
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 1,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    33 μl of tris chloride BCC pH 7 (3 M)
    566 μl of water (100 v/v)
    G9
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 1,000 20 w/v -
    • Sodium chloride 0.2 M -
    pH 6.2
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    40 μl of Sodium chloride (5 M)
    526 μl of water (100 v/v)
    G10
    • CAPS 0.1 M pH 10
    • Polyethylene glycol 8,000 20 w/v -
    • Sodium chloride 0.2 M -
    pH 10
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of CAPS pH 10 (1 M)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7
    • Jeffamine® ED-2001 30 v/v -
    pH 7
    600 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    300 μl of water (100 v/v)
    G12
    • Calcium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Calcium chloride (2 M)
    350 μl of water (100 v/v)
    H1
    • Calcium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 6.5
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Calcium chloride (2 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    350 μl of water (100 v/v)
    H2
    • Potassium chloride 0.2 M -
    • HEPES 0.05 M pH 7.5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
    pH 7.5
    50 μl of HEPES pH 7.5 (1 M)
    50 μl of Potassium chloride (4 M)
    700 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    200 μl of water (100 v/v)
    H3
    • Trimethylamine N-oxide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    200 μl of Trimethylamine N-oxide (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of water (100 v/v)
    H4
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of water (100 v/v)
    H5
    • di-Sodium succinate 0.1 M -
    • Polyethylene glycol 3,350 15 w/v -
    NA
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    83 μl of di-Sodium succinate pH 7 (1.2 M)
    616 μl of water (100 v/v)
    H6
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    pH 4
    100 μl of tri-Sodium citrate pH 4 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    H7
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 20,000 10 w/v -
    • 1,4-Dioxane 2 v/v -
    pH 9
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    40 μl of 1,4-Dioxane (50 v/v)
    100 μl of BICINE pH 9 (1 M)
    660 μl of water (100 v/v)
    H8
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    520 μl of water (100 v/v)
    H9
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2.5 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    833 μl of Ammonium sulfate (3 M)
    66 μl of water (100 v/v)
    H10
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium chloride (1 M)
    400 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2.5 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    833 μl of Ammonium sulfate (3 M)
    66 μl of water (100 v/v)
    H12
    • Zinc acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,000 10 w/v -
    pH 4.5
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Zinc acetate (2 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    666 μl of water (100 v/v)