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Supplemental Information

Screen Description

Created 2011-06-15.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 71
Internal diversity (Score mean dist)0.96
Internal diversity (Ion mean dist)0.93
Internal diversity (PEG mean dist)0.93
Internal diversity (Known pH mean dist)0.87
Internal diversity (Penalty mean dist)0.93

Screen Composition

Chemical classes bar chart
ingredient type MCSG-2
Buffer 104
Complex ingredient 2
Cryoprotectant 1
Organic (non-volatile) 8
Organic (volatile) 4
Polymer 53
Salt 115

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MCSG-2 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.33 μl of Potassium Sodium tartrate (1.5 M)
466.67 μl of water (100 v/v)
A2
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
A3
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
A4
  • di-Potassium hydrogen phosphate 1.2 M -
  • Sodium di-hydrogen phosphate 0.8 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
300.0 μl of di-Potassium hydrogen phosphate (4 M)
50.0 μl of Sodium acetate pH 4.5 (2 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
450.0 μl of water (100 v/v)
A5
  • Sodium di-hydrogen phosphate 1.2 M -
  • Lithium sulfate 0.2 M -
  • di-Potassium hydrogen phosphate 0.8 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
200.0 μl of di-Potassium hydrogen phosphate (4 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Sodium di-hydrogen phosphate (3 M)
200.0 μl of water (100 v/v)
A6
  • Ammonium sulfate 1.26 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
540.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 3,000 10 w/v -
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
660.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M pH 7.3
pH 7.3
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium formate pH 7.3 (1 M)
400.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate pH 6.3 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium chloride 0.2 M -
NA
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 600 40 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
800.0 μl of Polyethylene glycol 600 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
100.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 20,000 12 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium acetate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Potassium acetate (5 M)
560.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
B4
  • Sodium Potassium phosphate 1.8 M pH 8.2
pH 8.2
600.0 μl of Sodium Potassium phosphate pH 8.2 (3 M)
400.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
57.14 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
242.86 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.57 μl of Sodium formate (7 M)
571.43 μl of water (100 v/v)
B7
  • Sodium acetate 2.8 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
700.0 μl of Sodium acetate (4 M)
200.0 μl of water (100 v/v)
B8
  • Tacsimate 60 v/v -
NA
600.0 μl of Tacsimate pH 7 (100 v/v)
400.0 μl of water (100 v/v)
B9
  • Ammonium sulfate 1 M -
  • Polyethylene glycol 3,350 1 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
285.71 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
594.29 μl of water (100 v/v)
B10
  • di-Sodium malonate 1.1 M -
  • Jeffamine® ED-2001 0.5 v/v -
  • HEPES 0.1 M pH 7
pH 7
323.53 μl of di-Sodium malonate pH 7 (3.4 M)
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
566.47 μl of water (100 v/v)
B11
  • Polyethylene glycol 3,350 25 w/v -
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
B12
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
C1
  • di-Sodium succinate 0.8 M -
NA
666.67 μl of di-Sodium succinate pH 7 (1.2 M)
333.33 μl of water (100 v/v)
C2
  • Tacsimate 35 v/v -
NA
350.0 μl of Tacsimate pH 7 (100 v/v)
650.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Ammonium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.67 μl of tri-Ammonium citrate pH 7 (3 M)
533.33 μl of water (100 v/v)
C5
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate pH 7 (1.6 M)
475.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C7
  • di-Ammonium hydrogen phosphate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.57 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
471.43 μl of water (100 v/v)
C8
  • Magnesium sulfate 1.8 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
900.0 μl of Magnesium sulfate (2 M)
C9
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.57 μl of Sodium nitrate (7 M)
571.43 μl of water (100 v/v)
C11
  • di-Ammonium tartrate 1.3 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
650.0 μl of di-Ammonium tartrate (2 M)
250.0 μl of water (100 v/v)
C12
  • di-Ammonium tartrate 1.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
700.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
D1
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.57 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
471.43 μl of water (100 v/v)
D2
  • Ammonium sulfate 1.26 M -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
440.0 μl of water (100 v/v)
D3
  • Ammonium sulfate 2 M -
  • Lithium sulfate 0.2 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
228.57 μl of water (100 v/v)
D4
  • Polyethylene glycol 400 30 v/v -
  • Calcium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 4.5
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Sodium chloride 3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D6
  • Sodium acetate 2.8 M -
NA
700.0 μl of Sodium acetate pH 7 (4 M)
300.0 μl of water (100 v/v)
D7
  • di-Ammonium tartrate 1.1 M -
NA
550.0 μl of di-Ammonium tartrate pH 7 (2 M)
450.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.67 μl of Potassium nitrate pH 6.9 (3 M)
533.33 μl of water (100 v/v)
D9
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
100.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
400.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Lithium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.33 μl of tri-Lithium citrate (1.5 M)
466.67 μl of water (100 v/v)
D11
  • Lithium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
750.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium sulfate 0.2 M -
NA
57.14 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
542.86 μl of water (100 v/v)
E1
  • Sodium acetate 1.8 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
450.0 μl of Sodium acetate pH 7 (4 M)
450.0 μl of water (100 v/v)
E2
  • Sodium chloride 3.2 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
640.0 μl of Sodium chloride (5 M)
260.0 μl of water (100 v/v)
E3
  • Lithium sulfate 1.5 M -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 1.6 M -
  • 1,4-Dioxane 10 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
200.0 μl of 1,4-Dioxane (50 w/v)
457.14 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
242.86 μl of water (100 v/v)
E5
  • Polyethylene glycol 8,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
33.33 μl of Sodium acetate pH 4.5 (3 M)
266.67 μl of water (100 v/v)
E6
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
285.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
33.33 μl of Sodium acetate pH 4.5 (3 M)
680.96 μl of water (100 v/v)
E7
  • Polyethylene glycol 1,000 20 w/v -
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
E9
  • tri-Sodium citrate 1 M -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
625.0 μl of tri-Sodium citrate pH 7 (1.6 M)
275.0 μl of water (100 v/v)
E10
  • Sodium di-hydrogen phosphate 1.6 M -
  • di-Potassium hydrogen phosphate 0.4 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
100.0 μl of di-Potassium hydrogen phosphate (4 M)
400.0 μl of Sodium di-hydrogen phosphate (4 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
400.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 3,000 10 w/v -
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 400 30 v/v -
  • Lithium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
500.0 μl of water (100 v/v)
F1
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium chloride 0.2 M -
  • Imidazole 0.1 M pH 8
pH 8
285.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
574.29 μl of water (100 v/v)
F2
  • Sodium chloride 3 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
F3
  • Sodium chloride 3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
F4
  • Sodium Potassium phosphate 1 M -
NA
1000.0 μl of Sodium Potassium phosphate pH 7 (1 M)
F5
  • Poly(acrylic acid sodium salt) 5,100 22 w/v -
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Magnesium chloride (2 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
450.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.2 M -
NA
58.82 μl of di-Sodium malonate pH 7 (3.4 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
541.18 μl of water (100 v/v)
F7
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium DL-malate 0.15 M -
NA
50.0 μl of di-Sodium DL-malate pH 7 (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
550.0 μl of water (100 v/v)
F8
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium tartrate 0.2 M -
NA
133.33 μl of di-Sodium tartrate (1.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
466.67 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium nitrate 0.2 M -
NA
25.0 μl of Lithium nitrate (8 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
575.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium hydrogen phosphate 0.2 M -
NA
200.0 μl of di-Sodium hydrogen phosphate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
G2
  • di-Ammonium hydrogen phosphate 2.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
685.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
214.29 μl of water (100 v/v)
G3
  • Sodium di-hydrogen phosphate 1.6 M -
  • Sodium chloride 0.2 M -
  • di-Potassium hydrogen phosphate 0.4 M -
  • Imidazole 0.1 M pH 8
pH 8
100.0 μl of di-Potassium hydrogen phosphate (4 M)
100.0 μl of Imidazole pH 8 (1 M)
40.0 μl of Sodium chloride (5 M)
400.0 μl of Sodium di-hydrogen phosphate (4 M)
360.0 μl of water (100 v/v)
G4
  • Calcium acetate 0.2 M -
  • 2-Propanol 10 v/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Calcium acetate (1 M)
33.33 μl of MES pH 6 (3 M)
666.67 μl of water (100 v/v)
G5
  • Ammonium sulfate 1.26 M -
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
pH 9.5
360.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of CHES pH 9.5 (1 M)
40.0 μl of Sodium chloride (5 M)
500.0 μl of water (100 v/v)
G6
  • di-Ammonium hydrogen phosphate 1 M -
  • Sodium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
285.71 μl of di-Ammonium hydrogen phosphate (3.5 M)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
574.29 μl of water (100 v/v)
G7
  • Ethanol 15 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
150.0 μl of Ethanol (100 v/v)
33.33 μl of tris chloride BCC pH 7 (3 M)
816.67 μl of water (100 v/v)
G8
  • Polyethylene glycol 1,000 20 w/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.33 μl of tris chloride BCC pH 7 (3 M)
566.67 μl of water (100 v/v)
G9
  • Polyethylene glycol 1,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
33.33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
526.67 μl of water (100 v/v)
G10
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
G11
  • Jeffamine® ED-2001 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
G12
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H1
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
H2
  • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
  • Potassium chloride 0.2 M -
  • HEPES 0.05 M pH 7.5
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
50.0 μl of Potassium chloride (4 M)
200.0 μl of water (100 v/v)
H3
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Ammonium acetate (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 3,350 15 w/v -
  • di-Sodium succinate 0.1 M -
NA
83.33 μl of di-Sodium succinate pH 7 (1.2 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
616.67 μl of water (100 v/v)
H6
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
228.57 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
671.43 μl of water (100 v/v)
H7
  • Polyethylene glycol 20,000 10 w/v -
  • 1,4-Dioxane 2 v/v -
  • BICINE 0.1 M pH 9
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 400 30 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
H9
  • Ammonium sulfate 2.5 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
833.33 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
66.67 μl of water (100 v/v)
H10
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium chloride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
H11
  • Ammonium sulfate 2.5 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
833.33 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
66.67 μl of water (100 v/v)
H12
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 3,000 10 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
33.33 μl of Sodium acetate pH 4.5 (3 M)
100.0 μl of Zinc acetate (2 M)
666.67 μl of water (100 v/v)