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Supplemental Information

Screen Description

MCSG-4

Created 2011-06-15.

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MCSG-4

    Screen Structure

    MCSG-4

    Well Count 96
    Number of distinct ingredients 53
    Internal diversity (score mean dist) 0.947

    Screen Composition

    2025-06-14T13:44:27.880548 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MCSG-4
    Buffer 110
    Complex ingredient 3
    Cryoprotectant 2
    Organic (non-volatile) 18
    Organic (volatile) 13
    Polymer 57
    Salt 100

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MCSG-4 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,000 20 w/v -
    pH 4.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    550 μl of water (100 v/v)
    A2
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Sodium chloride (5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    510 μl of water (100 v/v)
    A3
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 3,000 10 w/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    700 μl of water (100 v/v)
    A4
    • CHES 0.1 M pH 9.5
    • Ethanol 15 v/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    150 μl of Ethanol (100 v/v)
    750 μl of water (100 v/v)
    A5
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • Polyethylene glycol 3,000 10 w/v -
    pH 6.2
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    766 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.1 M pH 8.5
    • 2-Propanol 10 v/v -
    pH 8.5
    100 μl of 2-Propanol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    800 μl of water (100 v/v)
    A7
    • Zinc acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Sodium chloride 2.5 M -
    pH 8
    500 μl of Sodium chloride (5 M)
    100 μl of Zinc acetate (2 M)
    100 μl of Imidazole pH 8 (1 M)
    300 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 4.5
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (2 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    600 μl of water (100 v/v)
    A9
    • Magnesium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 1,000 20 w/v -
    pH 6.5
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    420 μl of water (100 v/v)
    A10
    • Zinc acetate 0.2 M -
    • MES 0.1 M pH 6
    • Ethanol 15 v/v -
    pH 6
    33 μl of MES pH 6 (3 M)
    100 μl of Zinc acetate (2 M)
    150 μl of Ethanol (100 v/v)
    716 μl of water (100 v/v)
    A11
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 7
    33 μl of tris chloride BCC pH 7 (3 M)
    40 μl of Sodium chloride (5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    576 μl of water (100 v/v)
    A12
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium nitrate 4 M -
    pH 7
    571 μl of Sodium nitrate (7 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    328 μl of water (100 v/v)
    B1
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium DL-malate 2.4 M -
    pH 7
    800 μl of di-Sodium DL-malate pH 7 (3 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    100 μl of water (100 v/v)
    B2
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium Sodium tartrate 1.2 M -
    pH 7
    800 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    100 μl of water (100 v/v)
    B3
    • Sodium acetate 0.1 M pH 4.6
    • Lithium sulfate 1.5 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    750 μl of Lithium sulfate (2 M)
    150 μl of water (100 v/v)
    B4
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium formate 0.7 M -
    pH 7
    700 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    200 μl of water (100 v/v)
    B5
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium Sodium tartrate 0.6 M -
    pH 7
    400 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 0.7 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    437 μl of tri-Sodium citrate (1.6 M)
    462 μl of water (100 v/v)
    B7
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium nitrate 6 M -
    pH 8.5
    600 μl of Ammonium nitrate pH 6.3 (10 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    B8
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium chloride 1.5 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Ammonium chloride (5 M)
    600 μl of water (100 v/v)
    B9
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium formate 0.4 M -
    pH 7
    400 μl of Magnesium formate (1 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    B10
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium DL-malate 1.2 M -
    pH 7
    400 μl of di-Sodium DL-malate pH 7 (3 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    500 μl of water (100 v/v)
    B11
    • BIS-TRIS propane 0.1 M pH 7
    • Magnesium sulfate 1 M -
    pH 7
    500 μl of Magnesium sulfate (2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    400 μl of water (100 v/v)
    B12
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium formate 0.4 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of Magnesium formate (1 M)
    500 μl of water (100 v/v)
    C1
    • BIS-TRIS propane 0.1 M pH 7
    • di-Ammonium hydrogen citrate 2 M -
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    800 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of water (100 v/v)
    C2
    • tris chloride BCC 0.1 M pH 8.5
    • tri-Sodium citrate 1.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    750 μl of tri-Sodium citrate (1.6 M)
    150 μl of water (100 v/v)
    C3
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium formate 3.5 M -
    pH 8.5
    500 μl of Sodium formate (7 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    C4
    • Tacsimate 35 v/v -
    • BIS-TRIS propane 0.1 M pH 7
    pH 7
    350 μl of Tacsimate pH 7 (100 v/v)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    550 μl of water (100 v/v)
    C5
    • Sodium acetate 0.1 M pH 4.6
    • Magnesium sulfate 1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of Magnesium sulfate (2 M)
    400 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium hydrogen citrate 1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    400 μl of di-Ammonium hydrogen citrate (2.5 M)
    500 μl of water (100 v/v)
    C7
    • Sodium Potassium phosphate 1 M -
    NA
    333 μl of Sodium Potassium phosphate pH 5 (3 M)
    666 μl of water (100 v/v)
    C8
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium DL-malate 2.2 M -
    pH 7
    733 μl of di-Sodium DL-malate pH 7 (3 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    166 μl of water (100 v/v)
    C9
    • Tacsimate 60 v/v -
    • BIS-TRIS propane 0.1 M pH 7
    pH 7
    600 μl of Tacsimate pH 7 (100 v/v)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    300 μl of water (100 v/v)
    C10
    • Sodium Potassium phosphate 1 M pH 8.2
    pH 8.2
    333 μl of Sodium Potassium phosphate pH 8.2 (3 M)
    666 μl of water (100 v/v)
    C11
    • BIS-TRIS propane 0.1 M pH 7
    • di-Sodium succinate 0.5 M -
    pH 7
    416 μl of di-Sodium succinate pH 7 (1.2 M)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    483 μl of water (100 v/v)
    C12
    • Sodium acetate 0.1 M pH 4.6
    • di-Ammonium tartrate 1.1 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    550 μl of di-Ammonium tartrate pH 7 (2 M)
    350 μl of water (100 v/v)
    D1
    • Lithium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 50 v/v -
    pH 4.5
    500 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    33 μl of Sodium acetate pH 4.5 (3 M)
    366 μl of water (100 v/v)
    D2
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 40 v/v -
    pH 8.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    D3
    • Calcium chloride 0.14 M -
    • Sodium acetate 0.07 M pH 4.6
    • 2-Propanol 14 v/v -
    • Glycerol 30 v/v -
    pH 4.6
    70 μl of Sodium acetate pH 4.6 (1 M)
    140 μl of 2-Propanol (100 v/v)
    70 μl of Calcium chloride (2 M)
    300 μl of Glycerol (100 v/v)
    420 μl of water (100 v/v)
    D4
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium sulfate 1 M -
    pH 8.5
    500 μl of Magnesium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    D5
    • Tacsimate 15 v/v -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 3,350 2 w/v -
    pH 7
    150 μl of Tacsimate pH 7 (100 v/v)
    40 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    710 μl of water (100 v/v)
    D6
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    D7
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6
    • 1,4-Butanediol 20 v/v -
    pH 6
    100 μl of Lithium sulfate (2 M)
    500 μl of 1,4-Butanediol (40 v/v)
    33 μl of MES pH 6 (3 M)
    366 μl of water (100 v/v)
    D8
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Ethanol 15 v/v -
    pH 5.5
    100 μl of Lithium sulfate (2 M)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    150 μl of Ethanol (100 v/v)
    650 μl of water (100 v/v)
    D9
    • Sodium Potassium phosphate 0.1 M pH 6.2
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 6.2
    33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    616 μl of water (100 v/v)
    D10
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    pH 6
    100 μl of Lithium sulfate (2 M)
    33 μl of MES pH 6 (3 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    516 μl of water (100 v/v)
    D11
    • CAPS 0.1 M pH 10
    • Polyethylene glycol 400 30 v/v -
    pH 10
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of CAPS pH 10 (1 M)
    600 μl of water (100 v/v)
    D12
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 15 v/v -
    pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    150 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    670 μl of water (100 v/v)
    E1
    • tri-Sodium citrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 15 v/v -
    pH 8.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    625 μl of water (100 v/v)
    E2
    • Magnesium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 25 v/v -
    pH 4.6
    40 μl of Magnesium chloride (2.5 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    610 μl of water (100 v/v)
    E3
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 25 v/v -
    pH 8.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    550 μl of water (100 v/v)
    E4
    • Calcium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 400 30 v/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Calcium chloride (2 M)
    550 μl of water (100 v/v)
    E5
    • Sodium acetate 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of Sodium acetate (4 M)
    100 μl of MES pH 6.5 (1 M)
    575 μl of water (100 v/v)
    E6
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    40 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    560 μl of water (100 v/v)
    E7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 1,000 25 w/v -
    pH 7.5
    500 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    E8
    • Polyethylene glycol 1,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    E9
    • Polyethylene glycol 1,500 15 w/v -
    NA
    300 μl of Polyethylene glycol 1,500 (50 w/v)
    700 μl of water (100 v/v)
    E10
    • Nickel (II) chloride 10 mM -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Nickel (II) chloride (100 mM)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    E11
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    NA
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    500 μl of water (100 v/v)
    E12
    • Sodium acetate 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    25 μl of Sodium acetate (4 M)
    100 μl of MES pH 6.5 (1 M)
    275 μl of water (100 v/v)
    F1
    • Sodium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,000 20 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Sodium acetate (4 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    450 μl of water (100 v/v)
    F2
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 4 w/v -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    80 μl of Polyethylene glycol 4,000 (50 w/v)
    820 μl of water (100 v/v)
    F3
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Sodium acetate (4 M)
    530 μl of water (100 v/v)
    F4
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 16 w/v -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    F5
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    F6
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 7.5
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of Sodium acetate (4 M)
    435 μl of water (100 v/v)
    F7
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 6.5
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    320 μl of water (100 v/v)
    F8
    • Polyethylene glycol 4,000 30 w/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    F9
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 7.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium chloride (2 M)
    200 μl of water (100 v/v)
    F10
    • Polyethylene glycol 4,000 30 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    F11
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    pH 8.5
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Sodium acetate (4 M)
    250 μl of water (100 v/v)
    F12
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    220 μl of water (100 v/v)
    G1
    • Polyethylene glycol 4,000 35 w/v -
    NA
    700 μl of Polyethylene glycol 4,000 (50 w/v)
    300 μl of water (100 v/v)
    G2
    • Lithium chloride 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 8.5
    80 μl of Lithium chloride (10 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    660 μl of water (100 v/v)
    G3
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 20 v/v -
    NA
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    G4
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 10 w/v -
    • 2-Propanol 10 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    600 μl of water (100 v/v)
    G5
    • 2-Propanol 5 v/v -
    • Polyethylene glycol 4,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    650 μl of water (100 v/v)
    G6
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 15 w/v -
    NA
    57 μl of Ammonium sulfate (3.5 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    642 μl of water (100 v/v)
    G7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 15 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    542 μl of water (100 v/v)
    G8
    • Magnesium sulfate 0.2 M -
    • Polyethylene glycol 4,000 20 w/v -
    • Glycerol 10 v/v -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium sulfate (2 M)
    100 μl of Glycerol (100 v/v)
    400 μl of water (100 v/v)
    G9
    • tri-Sodium citrate 0.1 M -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 5 v/v -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of 2-Propanol (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    487 μl of water (100 v/v)
    G10
    • tri-Sodium citrate 0.1 M -
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 v/v -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    337 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    pH 7.5
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    G12
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 22 w/v -
    • Sodium acetate 0.1 M -
    NA
    57 μl of Ammonium sulfate (3.5 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Sodium acetate (4 M)
    477 μl of water (100 v/v)
    H1
    • Lithium sulfate 0.2 M -
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium acetate 0.1 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    25 μl of Sodium acetate (4 M)
    275 μl of water (100 v/v)
    H2
    • Sodium acetate 0.1 M -
    • Polyethylene glycol 4,000 25 w/v -
    • 2-Propanol 8 v/v -
    NA
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of 2-Propanol (100 v/v)
    25 μl of Sodium acetate (4 M)
    395 μl of water (100 v/v)
    H3
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    pH 6.5
    57 μl of Ammonium sulfate (3.5 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    242 μl of water (100 v/v)
    H4
    • Sodium chloride 2 M -
    • Polyethylene glycol 6,000 12 w/v -
    NA
    400 μl of Sodium chloride (5 M)
    240 μl of Polyethylene glycol 6,000 (50 w/v)
    360 μl of water (100 v/v)
    H5
    • Lithium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 6,000 25 w/v -
    pH 7.5
    10 μl of Lithium chloride (10 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 6,000 (50 w/v)
    390 μl of water (100 v/v)
    H6
    • tri-Sodium citrate 0.01 M -
    • Polyethylene glycol 6,000 33 w/v -
    NA
    660 μl of Polyethylene glycol 6,000 (50 w/v)
    6 μl of tri-Sodium citrate (1.6 M)
    333 μl of water (100 v/v)
    H7
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 0.5 M -
    NA
    250 μl of Lithium sulfate (2 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    710 μl of water (100 v/v)
    H8
    • Polyethylene glycol 8,000 2 w/v -
    • Lithium sulfate 1 M -
    NA
    500 μl of Lithium sulfate (2 M)
    40 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    H9
    • Lithium chloride 0.2 M -
    • Polyethylene glycol 8,000 8 w/v -
    • Magnesium sulfate 0.05 M -
    NA
    20 μl of Lithium chloride (10 M)
    25 μl of Magnesium sulfate (2 M)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    795 μl of water (100 v/v)
    H10
    • Zinc acetate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    • Imidazole 0.1 M pH 6.5
    pH 6.5
    100 μl of Imidazole pH 6.5 (1 M)
    100 μl of Zinc acetate (2 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    600 μl of water (100 v/v)
    H11
    • Calcium acetate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Calcium acetate (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    H12
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Magnesium acetate (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    600 μl of water (100 v/v)