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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
MCSG-4
Barcodes
MCSG-4
No barcodes found.
Screen Structure
MCSG-4
Well Count | 96 |
---|---|
Number of distinct ingredients | 53 |
Internal diversity (score mean dist) | 0.947 |
Screen Composition
ingredient type | MCSG-4 |
---|---|
Buffer | 110 |
Complex ingredient | 3 |
Cryoprotectant | 2 |
Organic (non-volatile) | 18 |
Organic (volatile) | 13 |
Polymer | 57 |
Salt | 100 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 550.0 μl of water (100 v/v) |
A2 |
| pH 7.5 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 510.0 μl of water (100 v/v) |
A3 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 700.0 μl of water (100 v/v) |
A4 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 150.0 μl of Ethanol (100 v/v) 750.0 μl of water (100 v/v) |
A5 |
| pH 6.2 | 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 766.7 μl of water (100 v/v) |
A6 |
| pH 8.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 800.0 μl of water (100 v/v) |
A7 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium chloride (5 M) 100.0 μl of Zinc acetate (2 M) 300.0 μl of water (100 v/v) |
A8 |
| pH 4.5 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 600.0 μl of water (100 v/v) |
A9 |
| pH 6.5 | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 420.0 μl of water (100 v/v) |
A10 |
| pH 6 | 150.0 μl of Ethanol (100 v/v) 33.3 μl of MES pH 6 (3 M) 100.0 μl of Zinc acetate (2 M) 716.7 μl of water (100 v/v) |
A11 |
| pH 7 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Sodium chloride (5 M) 33.3 μl of tris chloride BCC pH 7 (3 M) 576.7 μl of water (100 v/v) |
A12 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 571.4 μl of Sodium nitrate (7 M) 328.6 μl of water (100 v/v) |
B1 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 800.0 μl of di-Sodium DL-malate pH 7 (3 M) 100.0 μl of water (100 v/v) |
B2 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 800.0 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of water (100 v/v) |
B3 |
| pH 4.6 | 750.0 μl of Lithium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 150.0 μl of water (100 v/v) |
B4 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 700.0 μl of Magnesium formate (1 M) 200.0 μl of water (100 v/v) |
B5 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 400.0 μl of Potassium Sodium tartrate (1.5 M) 500.0 μl of water (100 v/v) |
B6 |
| pH 8.5 | 437.5 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 462.5 μl of water (100 v/v) |
B7 |
| pH 8.5 | 600.0 μl of Ammonium nitrate pH 6.3 (10 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
B8 |
| pH 4.6 | 300.0 μl of Ammonium chloride (5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 600.0 μl of water (100 v/v) |
B9 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 400.0 μl of Magnesium formate (1 M) 500.0 μl of water (100 v/v) |
B10 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 400.0 μl of di-Sodium DL-malate pH 7 (3 M) 500.0 μl of water (100 v/v) |
B11 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 500.0 μl of Magnesium sulfate (2 M) 400.0 μl of water (100 v/v) |
B12 |
| pH 4.6 | 400.0 μl of Magnesium formate (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v) |
C1 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 800.0 μl of di-Ammonium hydrogen citrate (2.5 M) 100.0 μl of water (100 v/v) |
C2 |
| pH 8.5 | 750.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 150.0 μl of water (100 v/v) |
C3 |
| pH 8.5 | 500.0 μl of Sodium formate (7 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
C4 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 350.0 μl of Tacsimate pH 7 (100 v/v) 550.0 μl of water (100 v/v) |
C5 |
| pH 4.6 | 500.0 μl of Magnesium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of water (100 v/v) |
C6 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 400.0 μl of di-Ammonium hydrogen citrate (2.5 M) 500.0 μl of water (100 v/v) |
C7 |
| NA | 333.3 μl of Sodium Potassium phosphate pH 5 (3 M) 666.7 μl of water (100 v/v) |
C8 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 733.3 μl of di-Sodium DL-malate pH 7 (3 M) 166.7 μl of water (100 v/v) |
C9 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 600.0 μl of Tacsimate pH 7 (100 v/v) 300.0 μl of water (100 v/v) |
C10 |
| pH 8.2 | 333.3 μl of Sodium Potassium phosphate pH 8.2 (3 M) 666.7 μl of water (100 v/v) |
C11 |
| pH 7 | 100.0 μl of BIS-TRIS propane pH 7 (1 M) 416.7 μl of di-Sodium succinate pH 7 (1.2 M) 483.3 μl of water (100 v/v) |
C12 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of di-Ammonium tartrate pH 7 (2 M) 350.0 μl of water (100 v/v) |
D1 |
| pH 4.5 | 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 366.7 μl of water (100 v/v) |
D2 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D3 |
| pH 4.6 | 140.0 μl of 2-Propanol (100 v/v) 70.0 μl of Calcium chloride (2 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 420.0 μl of water (100 v/v) |
D4 |
| pH 8.5 | 500.0 μl of Magnesium sulfate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D5 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 40.0 μl of Polyethylene glycol 3,350 (50 w/v) 150.0 μl of Tacsimate pH 7 (100 v/v) 710.0 μl of water (100 v/v) |
D6 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 250.0 μl of water (100 v/v) |
D7 |
| pH 6 | 500.0 μl of 1,4-Butanediol (40 v/v) 100.0 μl of Lithium sulfate (2 M) 33.3 μl of MES pH 6 (3 M) 366.7 μl of water (100 v/v) |
D8 |
| pH 5.5 | 150.0 μl of Ethanol (100 v/v) 100.0 μl of Lithium sulfate (2 M) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 650.0 μl of water (100 v/v) |
D9 |
| pH 6.2 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 616.7 μl of water (100 v/v) |
D10 |
| pH 6 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Lithium sulfate (2 M) 33.3 μl of MES pH 6 (3 M) 516.7 μl of water (100 v/v) |
D11 |
| pH 10 | 100.0 μl of CAPS pH 10 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 600.0 μl of water (100 v/v) |
D12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 150.0 μl of Polyethylene glycol 400 (100 v/v) 670.0 μl of water (100 v/v) |
E1 |
| pH 8.5 | 150.0 μl of Polyethylene glycol 400 (100 v/v) 125.0 μl of tri-Sodium citrate (1.6 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 625.0 μl of water (100 v/v) |
E2 |
| pH 4.6 | 40.0 μl of Magnesium chloride (2.5 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 610.0 μl of water (100 v/v) |
E3 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 550.0 μl of water (100 v/v) |
E4 |
| pH 4.6 | 50.0 μl of Calcium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of water (100 v/v) |
E5 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 25.0 μl of Sodium acetate (4 M) 575.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 40.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 560.0 μl of water (100 v/v) |
E7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of water (100 v/v) |
E8 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
E9 |
| NA | 300.0 μl of Polyethylene glycol 1,500 (50 w/v) 700.0 μl of water (100 v/v) |
E10 |
| pH 8.5 | 100.0 μl of Nickel (II) chloride (100 mM) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
E11 |
| NA | 500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of water (100 v/v) |
E12 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 25.0 μl of Sodium acetate (4 M) 275.0 μl of water (100 v/v) |
F1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 50.0 μl of Sodium acetate (4 M) 450.0 μl of water (100 v/v) |
F2 |
| pH 4.6 | 80.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 820.0 μl of water (100 v/v) |
F3 |
| pH 8.5 | 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 530.0 μl of water (100 v/v) |
F4 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
F5 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
F6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Sodium acetate (4 M) 435.0 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 320.0 μl of water (100 v/v) |
F8 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v) |
F9 |
| pH 7.5 | 100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 200.0 μl of water (100 v/v) |
F10 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
F11 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium acetate (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
F12 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
G1 |
| NA | 700.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v) |
G2 |
| pH 8.5 | 80.0 μl of Lithium chloride (10 M) 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 660.0 μl of water (100 v/v) |
G3 |
| NA | 200.0 μl of 2-Propanol (100 v/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 600.0 μl of water (100 v/v) |
G4 |
| pH 5.6 | 100.0 μl of 2-Propanol (100 v/v) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 600.0 μl of water (100 v/v) |
G5 |
| pH 7.5 | 50.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (50 w/v) 650.0 μl of water (100 v/v) |
G6 |
| NA | 57.1 μl of Ammonium sulfate (3.5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 642.9 μl of water (100 v/v) |
G7 |
| pH 5.6 | 57.1 μl of Ammonium sulfate (3.5 M) 300.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 542.9 μl of water (100 v/v) |
G8 |
| NA | 100.0 μl of Glycerol (100 v/v) 100.0 μl of Magnesium sulfate (2 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
G9 |
| NA | 50.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 62.5 μl of tri-Sodium citrate (1.6 M) 487.5 μl of water (100 v/v) |
G10 |
| NA | 200.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 62.5 μl of tri-Sodium citrate (1.6 M) 337.5 μl of water (100 v/v) |
G11 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
G12 |
| NA | 57.1 μl of Ammonium sulfate (3.5 M) 440.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Sodium acetate (4 M) 477.9 μl of water (100 v/v) |
H1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Sodium acetate (4 M) 275.0 μl of water (100 v/v) |
H2 |
| NA | 80.0 μl of 2-Propanol (100 v/v) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 25.0 μl of Sodium acetate (4 M) 395.0 μl of water (100 v/v) |
H3 |
| pH 6.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 242.9 μl of water (100 v/v) |
H4 |
| NA | 240.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
H5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 10.0 μl of Lithium chloride (10 M) 500.0 μl of Polyethylene glycol 6,000 (50 w/v) 390.0 μl of water (100 v/v) |
H6 |
| NA | 660.0 μl of Polyethylene glycol 6,000 (50 w/v) 6.2 μl of tri-Sodium citrate (1.6 M) 333.8 μl of water (100 v/v) |
H7 |
| NA | 250.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 710.0 μl of water (100 v/v) |
H8 |
| NA | 500.0 μl of Lithium sulfate (2 M) 40.0 μl of Polyethylene glycol 8,000 (50 w/v) 460.0 μl of water (100 v/v) |
H9 |
| NA | 20.0 μl of Lithium chloride (10 M) 25.0 μl of Magnesium sulfate (2 M) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 795.0 μl of water (100 v/v) |
H10 |
| pH 6.5 | 100.0 μl of Imidazole pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 600.0 μl of water (100 v/v) |
H11 |
| pH 7.5 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H12 |
| NA | 200.0 μl of Magnesium acetate (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |