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Supplemental Information

Screen Description

MCSG-4

Created 2011-06-15.

Export

Barcodes

MCSG-4

No barcodes found.

Screen Structure

MCSG-4

Well Count 96
Number of distinct ingredients 53
Internal diversity (score mean dist) 0.947

Screen Composition

2025-08-16T09:05:43.615340 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MCSG-4
Buffer 110
Complex ingredient 3
Cryoprotectant 2
Organic (non-volatile) 18
Organic (volatile) 13
Polymer 57
Salt 100

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MCSG-4 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,000 20 w/v -
pH 4.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
550.0 μl of water (100 v/v)
A2
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 7.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Sodium chloride (5 M)
510.0 μl of water (100 v/v)
A3
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 3,000 10 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
700.0 μl of water (100 v/v)
A4
  • CHES 0.1 M pH 9.5
  • Ethanol 15 v/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
150.0 μl of Ethanol (100 v/v)
750.0 μl of water (100 v/v)
A5
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 3,000 10 w/v -
pH 6.2
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
766.7 μl of water (100 v/v)
A6
  • tris chloride BCC 0.1 M pH 8.5
  • 2-Propanol 10 v/v -
pH 8.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
800.0 μl of water (100 v/v)
A7
  • Zinc acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Sodium chloride 2.5 M -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
500.0 μl of Sodium chloride (5 M)
100.0 μl of Zinc acetate (2 M)
300.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.1 M pH 4.5
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 4.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
600.0 μl of water (100 v/v)
A9
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 1,000 20 w/v -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
420.0 μl of water (100 v/v)
A10
  • Zinc acetate 0.2 M -
  • MES 0.1 M pH 6
  • Ethanol 15 v/v -
pH 6
150.0 μl of Ethanol (100 v/v)
33.3 μl of MES pH 6 (3 M)
100.0 μl of Zinc acetate (2 M)
716.7 μl of water (100 v/v)
A11
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 7
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Sodium chloride (5 M)
33.3 μl of tris chloride BCC pH 7 (3 M)
576.7 μl of water (100 v/v)
A12
  • BIS-TRIS propane 0.1 M pH 7
  • Sodium nitrate 4 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
571.4 μl of Sodium nitrate (7 M)
328.6 μl of water (100 v/v)
B1
  • BIS-TRIS propane 0.1 M pH 7
  • di-Sodium DL-malate 2.4 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
800.0 μl of di-Sodium DL-malate pH 7 (3 M)
100.0 μl of water (100 v/v)
B2
  • BIS-TRIS propane 0.1 M pH 7
  • Potassium Sodium tartrate 1.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
800.0 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M pH 4.6
  • Lithium sulfate 1.5 M -
pH 4.6
750.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
150.0 μl of water (100 v/v)
B4
  • BIS-TRIS propane 0.1 M pH 7
  • Magnesium formate 0.7 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
700.0 μl of Magnesium formate (1 M)
200.0 μl of water (100 v/v)
B5
  • BIS-TRIS propane 0.1 M pH 7
  • Potassium Sodium tartrate 0.6 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
400.0 μl of Potassium Sodium tartrate (1.5 M)
500.0 μl of water (100 v/v)
B6
  • tris chloride BCC 0.1 M pH 8.5
  • tri-Sodium citrate 0.7 M -
pH 8.5
437.5 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
462.5 μl of water (100 v/v)
B7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium nitrate 6 M -
pH 8.5
600.0 μl of Ammonium nitrate pH 6.3 (10 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B8
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium chloride 1.5 M -
pH 4.6
300.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
600.0 μl of water (100 v/v)
B9
  • BIS-TRIS propane 0.1 M pH 7
  • Magnesium formate 0.4 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
400.0 μl of Magnesium formate (1 M)
500.0 μl of water (100 v/v)
B10
  • BIS-TRIS propane 0.1 M pH 7
  • di-Sodium DL-malate 1.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
400.0 μl of di-Sodium DL-malate pH 7 (3 M)
500.0 μl of water (100 v/v)
B11
  • BIS-TRIS propane 0.1 M pH 7
  • Magnesium sulfate 1 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
500.0 μl of Magnesium sulfate (2 M)
400.0 μl of water (100 v/v)
B12
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium formate 0.4 M -
pH 4.6
400.0 μl of Magnesium formate (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
C1
  • BIS-TRIS propane 0.1 M pH 7
  • di-Ammonium hydrogen citrate 2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
800.0 μl of di-Ammonium hydrogen citrate (2.5 M)
100.0 μl of water (100 v/v)
C2
  • tris chloride BCC 0.1 M pH 8.5
  • tri-Sodium citrate 1.2 M -
pH 8.5
750.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
150.0 μl of water (100 v/v)
C3
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium formate 3.5 M -
pH 8.5
500.0 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
C4
  • Tacsimate 35 v/v -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
350.0 μl of Tacsimate pH 7 (100 v/v)
550.0 μl of water (100 v/v)
C5
  • Sodium acetate 0.1 M pH 4.6
  • Magnesium sulfate 1 M -
pH 4.6
500.0 μl of Magnesium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of water (100 v/v)
C6
  • Sodium acetate 0.1 M pH 4.6
  • di-Ammonium hydrogen citrate 1 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
400.0 μl of di-Ammonium hydrogen citrate (2.5 M)
500.0 μl of water (100 v/v)
C7
  • Sodium Potassium phosphate 1 M -
NA
333.3 μl of Sodium Potassium phosphate pH 5 (3 M)
666.7 μl of water (100 v/v)
C8
  • BIS-TRIS propane 0.1 M pH 7
  • di-Sodium DL-malate 2.2 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
733.3 μl of di-Sodium DL-malate pH 7 (3 M)
166.7 μl of water (100 v/v)
C9
  • Tacsimate 60 v/v -
  • BIS-TRIS propane 0.1 M pH 7
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
600.0 μl of Tacsimate pH 7 (100 v/v)
300.0 μl of water (100 v/v)
C10
  • Sodium Potassium phosphate 1 M pH 8.2
pH 8.2
333.3 μl of Sodium Potassium phosphate pH 8.2 (3 M)
666.7 μl of water (100 v/v)
C11
  • BIS-TRIS propane 0.1 M pH 7
  • di-Sodium succinate 0.5 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
416.7 μl of di-Sodium succinate pH 7 (1.2 M)
483.3 μl of water (100 v/v)
C12
  • Sodium acetate 0.1 M pH 4.6
  • di-Ammonium tartrate 1.1 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of di-Ammonium tartrate pH 7 (2 M)
350.0 μl of water (100 v/v)
D1
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 50 v/v -
pH 4.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate pH 4.5 (3 M)
366.7 μl of water (100 v/v)
D2
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 40 v/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D3
  • Calcium chloride 0.14 M -
  • Sodium acetate 0.07 M pH 4.6
  • 2-Propanol 14 v/v -
  • Glycerol 30 v/v -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
70.0 μl of Calcium chloride (2 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
420.0 μl of water (100 v/v)
D4
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium sulfate 1 M -
pH 8.5
500.0 μl of Magnesium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Tacsimate 15 v/v -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 3,350 2 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
40.0 μl of Polyethylene glycol 3,350 (50 w/v)
150.0 μl of Tacsimate pH 7 (100 v/v)
710.0 μl of water (100 v/v)
D6
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
D7
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
  • 1,4-Butanediol 20 v/v -
pH 6
500.0 μl of 1,4-Butanediol (40 v/v)
100.0 μl of Lithium sulfate (2 M)
33.3 μl of MES pH 6 (3 M)
366.7 μl of water (100 v/v)
D8
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Ethanol 15 v/v -
pH 5.5
150.0 μl of Ethanol (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
650.0 μl of water (100 v/v)
D9
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 6.2
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
616.7 μl of water (100 v/v)
D10
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Lithium sulfate (2 M)
33.3 μl of MES pH 6 (3 M)
516.7 μl of water (100 v/v)
D11
  • CAPS 0.1 M pH 10
  • Polyethylene glycol 400 30 v/v -
pH 10
100.0 μl of CAPS pH 10 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
D12
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 15 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
670.0 μl of water (100 v/v)
E1
  • tri-Sodium citrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 15 v/v -
pH 8.5
150.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
625.0 μl of water (100 v/v)
E2
  • Magnesium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 25 v/v -
pH 4.6
40.0 μl of Magnesium chloride (2.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
610.0 μl of water (100 v/v)
E3
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 25 v/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
550.0 μl of water (100 v/v)
E4
  • Calcium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 400 30 v/v -
pH 4.6
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
E5
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Sodium acetate (4 M)
575.0 μl of water (100 v/v)
E6
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
E7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 1,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 1,500 15 w/v -
NA
300.0 μl of Polyethylene glycol 1,500 (50 w/v)
700.0 μl of water (100 v/v)
E10
  • Nickel (II) chloride 10 mM -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
100.0 μl of Nickel (II) chloride (100 mM)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E11
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
E12
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Sodium acetate (4 M)
275.0 μl of water (100 v/v)
F1
  • Sodium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
50.0 μl of Sodium acetate (4 M)
450.0 μl of water (100 v/v)
F2
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 4 w/v -
pH 4.6
80.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
820.0 μl of water (100 v/v)
F3
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
530.0 μl of water (100 v/v)
F4
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 16 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
F5
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
F6
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Sodium acetate (4 M)
435.0 μl of water (100 v/v)
F7
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 4,000 25 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
320.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 4,000 30 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
F9
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 30 w/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
F11
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 4,000 35 w/v -
NA
700.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
G2
  • Lithium chloride 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 8 w/v -
pH 8.5
80.0 μl of Lithium chloride (10 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
660.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 20 v/v -
NA
200.0 μl of 2-Propanol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
600.0 μl of water (100 v/v)
G4
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 10 w/v -
  • 2-Propanol 10 v/v -
pH 5.6
100.0 μl of 2-Propanol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
600.0 μl of water (100 v/v)
G5
  • 2-Propanol 5 v/v -
  • Polyethylene glycol 4,000 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
650.0 μl of water (100 v/v)
G6
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 15 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
642.9 μl of water (100 v/v)
G7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 15 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
542.9 μl of water (100 v/v)
G8
  • Magnesium sulfate 0.2 M -
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 10 v/v -
NA
100.0 μl of Glycerol (100 v/v)
100.0 μl of Magnesium sulfate (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
G9
  • tri-Sodium citrate 0.1 M -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 5 v/v -
NA
50.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
62.5 μl of tri-Sodium citrate (1.6 M)
487.5 μl of water (100 v/v)
G10
  • tri-Sodium citrate 0.1 M -
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
NA
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
62.5 μl of tri-Sodium citrate (1.6 M)
337.5 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
G12
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 22 w/v -
  • Sodium acetate 0.1 M -
NA
57.1 μl of Ammonium sulfate (3.5 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Sodium acetate (4 M)
477.9 μl of water (100 v/v)
H1
  • Lithium sulfate 0.2 M -
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Sodium acetate (4 M)
275.0 μl of water (100 v/v)
H2
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 4,000 25 w/v -
  • 2-Propanol 8 v/v -
NA
80.0 μl of 2-Propanol (100 v/v)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Sodium acetate (4 M)
395.0 μl of water (100 v/v)
H3
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
242.9 μl of water (100 v/v)
H4
  • Sodium chloride 2 M -
  • Polyethylene glycol 6,000 12 w/v -
NA
240.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
H5
  • Lithium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 6,000 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Lithium chloride (10 M)
500.0 μl of Polyethylene glycol 6,000 (50 w/v)
390.0 μl of water (100 v/v)
H6
  • tri-Sodium citrate 0.01 M -
  • Polyethylene glycol 6,000 33 w/v -
NA
660.0 μl of Polyethylene glycol 6,000 (50 w/v)
6.2 μl of tri-Sodium citrate (1.6 M)
333.8 μl of water (100 v/v)
H7
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 0.5 M -
NA
250.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
710.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 8,000 2 w/v -
  • Lithium sulfate 1 M -
NA
500.0 μl of Lithium sulfate (2 M)
40.0 μl of Polyethylene glycol 8,000 (50 w/v)
460.0 μl of water (100 v/v)
H9
  • Lithium chloride 0.2 M -
  • Polyethylene glycol 8,000 8 w/v -
  • Magnesium sulfate 0.05 M -
NA
20.0 μl of Lithium chloride (10 M)
25.0 μl of Magnesium sulfate (2 M)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
795.0 μl of water (100 v/v)
H10
  • Zinc acetate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 6.5
pH 6.5
100.0 μl of Imidazole pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Zinc acetate (2 M)
600.0 μl of water (100 v/v)
H11
  • Calcium acetate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Calcium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H12
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Magnesium acetate (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)