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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
MCSG-4
Barcodes
MCSG-4
Screen Structure
MCSG-4
Well Count | 96 |
---|---|
Number of distinct ingredients | 53 |
Internal diversity (score mean dist) | 0.947 |
Screen Composition
ingredient type | MCSG-4 |
---|---|
Buffer | 110 |
Complex ingredient | 3 |
Cryoprotectant | 2 |
Organic (non-volatile) | 18 |
Organic (volatile) | 13 |
Polymer | 57 |
Salt | 100 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 400 μl of Polyethylene glycol 3,000 (50 w/v) 50 μl of Sodium acetate pH 4.5 (2 M) 550 μl of water (100 v/v) |
A2 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 40 μl of Sodium chloride (5 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 510 μl of water (100 v/v) |
A3 |
| pH 9.5 | 100 μl of CHES pH 9.5 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 700 μl of water (100 v/v) |
A4 |
| pH 9.5 | 100 μl of CHES pH 9.5 (1 M) 150 μl of Ethanol (100 v/v) 750 μl of water (100 v/v) |
A5 |
| pH 6.2 | 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 766 μl of water (100 v/v) |
A6 |
| pH 8.5 | 100 μl of 2-Propanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 800 μl of water (100 v/v) |
A7 |
| pH 8 | 500 μl of Sodium chloride (5 M) 100 μl of Zinc acetate (2 M) 100 μl of Imidazole pH 8 (1 M) 300 μl of water (100 v/v) |
A8 |
| pH 4.5 | 50 μl of Sodium acetate pH 4.5 (2 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 600 μl of water (100 v/v) |
A9 |
| pH 6.5 | 80 μl of Magnesium chloride (2.5 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 420 μl of water (100 v/v) |
A10 |
| pH 6 | 33 μl of MES pH 6 (3 M) 100 μl of Zinc acetate (2 M) 150 μl of Ethanol (100 v/v) 716 μl of water (100 v/v) |
A11 |
| pH 7 | 33 μl of tris chloride BCC pH 7 (3 M) 40 μl of Sodium chloride (5 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 576 μl of water (100 v/v) |
A12 |
| pH 7 | 571 μl of Sodium nitrate (7 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 328 μl of water (100 v/v) |
B1 |
| pH 7 | 800 μl of di-Sodium DL-malate pH 7 (3 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 100 μl of water (100 v/v) |
B2 |
| pH 7 | 800 μl of Potassium Sodium tartrate (1.5 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 100 μl of water (100 v/v) |
B3 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 750 μl of Lithium sulfate (2 M) 150 μl of water (100 v/v) |
B4 |
| pH 7 | 700 μl of Magnesium formate (1 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 200 μl of water (100 v/v) |
B5 |
| pH 7 | 400 μl of Potassium Sodium tartrate (1.5 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 500 μl of water (100 v/v) |
B6 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 437 μl of tri-Sodium citrate (1.6 M) 462 μl of water (100 v/v) |
B7 |
| pH 8.5 | 600 μl of Ammonium nitrate pH 6.3 (10 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
B8 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Ammonium chloride (5 M) 600 μl of water (100 v/v) |
B9 |
| pH 7 | 400 μl of Magnesium formate (1 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 500 μl of water (100 v/v) |
B10 |
| pH 7 | 400 μl of di-Sodium DL-malate pH 7 (3 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 500 μl of water (100 v/v) |
B11 |
| pH 7 | 500 μl of Magnesium sulfate (2 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 400 μl of water (100 v/v) |
B12 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of Magnesium formate (1 M) 500 μl of water (100 v/v) |
C1 |
| pH 7 | 100 μl of BIS-TRIS propane pH 7 (1 M) 800 μl of di-Ammonium hydrogen citrate (2.5 M) 100 μl of water (100 v/v) |
C2 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 750 μl of tri-Sodium citrate (1.6 M) 150 μl of water (100 v/v) |
C3 |
| pH 8.5 | 500 μl of Sodium formate (7 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
C4 |
| pH 7 | 350 μl of Tacsimate pH 7 (100 v/v) 100 μl of BIS-TRIS propane pH 7 (1 M) 550 μl of water (100 v/v) |
C5 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 500 μl of Magnesium sulfate (2 M) 400 μl of water (100 v/v) |
C6 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of di-Ammonium hydrogen citrate (2.5 M) 500 μl of water (100 v/v) |
C7 |
| NA | 333 μl of Sodium Potassium phosphate pH 5 (3 M) 666 μl of water (100 v/v) |
C8 |
| pH 7 | 733 μl of di-Sodium DL-malate pH 7 (3 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 166 μl of water (100 v/v) |
C9 |
| pH 7 | 600 μl of Tacsimate pH 7 (100 v/v) 100 μl of BIS-TRIS propane pH 7 (1 M) 300 μl of water (100 v/v) |
C10 |
| pH 8.2 | 333 μl of Sodium Potassium phosphate pH 8.2 (3 M) 666 μl of water (100 v/v) |
C11 |
| pH 7 | 416 μl of di-Sodium succinate pH 7 (1.2 M) 100 μl of BIS-TRIS propane pH 7 (1 M) 483 μl of water (100 v/v) |
C12 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 550 μl of di-Ammonium tartrate pH 7 (2 M) 350 μl of water (100 v/v) |
D1 |
| pH 4.5 | 500 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Lithium sulfate (2 M) 33 μl of Sodium acetate pH 4.5 (3 M) 366 μl of water (100 v/v) |
D2 |
| pH 8.5 | 400 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
D3 |
| pH 4.6 | 70 μl of Sodium acetate pH 4.6 (1 M) 140 μl of 2-Propanol (100 v/v) 70 μl of Calcium chloride (2 M) 300 μl of Glycerol (100 v/v) 420 μl of water (100 v/v) |
D4 |
| pH 8.5 | 500 μl of Magnesium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
D5 |
| pH 7 | 150 μl of Tacsimate pH 7 (100 v/v) 40 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of HEPES pH 7 (1 M) 710 μl of water (100 v/v) |
D6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 250 μl of water (100 v/v) |
D7 |
| pH 6 | 100 μl of Lithium sulfate (2 M) 500 μl of 1,4-Butanediol (40 v/v) 33 μl of MES pH 6 (3 M) 366 μl of water (100 v/v) |
D8 |
| pH 5.5 | 100 μl of Lithium sulfate (2 M) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 150 μl of Ethanol (100 v/v) 650 μl of water (100 v/v) |
D9 |
| pH 6.2 | 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 616 μl of water (100 v/v) |
D10 |
| pH 6 | 100 μl of Lithium sulfate (2 M) 33 μl of MES pH 6 (3 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 516 μl of water (100 v/v) |
D11 |
| pH 10 | 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of CAPS pH 10 (1 M) 600 μl of water (100 v/v) |
D12 |
| pH 7.5 | 80 μl of Magnesium chloride (2.5 M) 150 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 670 μl of water (100 v/v) |
E1 |
| pH 8.5 | 150 μl of Polyethylene glycol 400 (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 125 μl of tri-Sodium citrate (1.6 M) 625 μl of water (100 v/v) |
E2 |
| pH 4.6 | 40 μl of Magnesium chloride (2.5 M) 100 μl of Sodium acetate pH 4.6 (1 M) 250 μl of Polyethylene glycol 400 (100 v/v) 610 μl of water (100 v/v) |
E3 |
| pH 8.5 | 250 μl of Polyethylene glycol 400 (100 v/v) 100 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 550 μl of water (100 v/v) |
E4 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 50 μl of Calcium chloride (2 M) 550 μl of water (100 v/v) |
E5 |
| pH 6.5 | 300 μl of Polyethylene glycol 400 (100 v/v) 25 μl of Sodium acetate (4 M) 100 μl of MES pH 6.5 (1 M) 575 μl of water (100 v/v) |
E6 |
| pH 6.5 | 40 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of MES pH 6.5 (1 M) 560 μl of water (100 v/v) |
E7 |
| pH 7.5 | 500 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v) |
E8 |
| pH 8.5 | 600 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
E9 |
| NA | 300 μl of Polyethylene glycol 1,500 (50 w/v) 700 μl of water (100 v/v) |
E10 |
| pH 8.5 | 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of Nickel (II) chloride (100 mM) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
E11 |
| NA | 500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500 μl of water (100 v/v) |
E12 |
| pH 6.5 | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 25 μl of Sodium acetate (4 M) 100 μl of MES pH 6.5 (1 M) 275 μl of water (100 v/v) |
F1 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 50 μl of Sodium acetate (4 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 450 μl of water (100 v/v) |
F2 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 80 μl of Polyethylene glycol 4,000 (50 w/v) 820 μl of water (100 v/v) |
F3 |
| pH 8.5 | 320 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Sodium acetate (4 M) 530 μl of water (100 v/v) |
F4 |
| pH 8.5 | 80 μl of Magnesium chloride (2.5 M) 320 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v) |
F5 |
| pH 8.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of water (100 v/v) |
F6 |
| pH 7.5 | 440 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 25 μl of Sodium acetate (4 M) 435 μl of water (100 v/v) |
F7 |
| pH 6.5 | 80 μl of Magnesium chloride (2.5 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 320 μl of water (100 v/v) |
F8 |
| pH 6.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 300 μl of water (100 v/v) |
F9 |
| pH 7.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Calcium chloride (2 M) 200 μl of water (100 v/v) |
F10 |
| pH 8.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of water (100 v/v) |
F11 |
| pH 8.5 | 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 50 μl of Sodium acetate (4 M) 250 μl of water (100 v/v) |
F12 |
| pH 8.5 | 80 μl of Magnesium chloride (2.5 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 220 μl of water (100 v/v) |
G1 |
| NA | 700 μl of Polyethylene glycol 4,000 (50 w/v) 300 μl of water (100 v/v) |
G2 |
| pH 8.5 | 80 μl of Lithium chloride (10 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 660 μl of water (100 v/v) |
G3 |
| NA | 200 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v) |
G4 |
| pH 5.6 | 100 μl of tri-Sodium citrate pH 5.6 (1 M) 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of 2-Propanol (100 v/v) 600 μl of water (100 v/v) |
G5 |
| pH 7.5 | 200 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 50 μl of 2-Propanol (100 v/v) 650 μl of water (100 v/v) |
G6 |
| NA | 57 μl of Ammonium sulfate (3.5 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 642 μl of water (100 v/v) |
G7 |
| pH 5.6 | 100 μl of tri-Sodium citrate pH 5.6 (1 M) 57 μl of Ammonium sulfate (3.5 M) 300 μl of Polyethylene glycol 4,000 (50 w/v) 542 μl of water (100 v/v) |
G8 |
| NA | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Magnesium sulfate (2 M) 100 μl of Glycerol (100 v/v) 400 μl of water (100 v/v) |
G9 |
| NA | 400 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of 2-Propanol (100 v/v) 62 μl of tri-Sodium citrate (1.6 M) 487 μl of water (100 v/v) |
G10 |
| NA | 400 μl of Polyethylene glycol 4,000 (50 w/v) 200 μl of 2-Propanol (100 v/v) 62 μl of tri-Sodium citrate (1.6 M) 337 μl of water (100 v/v) |
G11 |
| pH 7.5 | 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of 2-Propanol (100 v/v) 400 μl of water (100 v/v) |
G12 |
| NA | 57 μl of Ammonium sulfate (3.5 M) 440 μl of Polyethylene glycol 4,000 (50 w/v) 25 μl of Sodium acetate (4 M) 477 μl of water (100 v/v) |
H1 |
| pH 7.5 | 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Lithium sulfate (2 M) 25 μl of Sodium acetate (4 M) 275 μl of water (100 v/v) |
H2 |
| NA | 500 μl of Polyethylene glycol 4,000 (50 w/v) 80 μl of 2-Propanol (100 v/v) 25 μl of Sodium acetate (4 M) 395 μl of water (100 v/v) |
H3 |
| pH 6.5 | 57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 242 μl of water (100 v/v) |
H4 |
| NA | 400 μl of Sodium chloride (5 M) 240 μl of Polyethylene glycol 6,000 (50 w/v) 360 μl of water (100 v/v) |
H5 |
| pH 7.5 | 10 μl of Lithium chloride (10 M) 100 μl of HEPES pH 7.5 (1 M) 500 μl of Polyethylene glycol 6,000 (50 w/v) 390 μl of water (100 v/v) |
H6 |
| NA | 660 μl of Polyethylene glycol 6,000 (50 w/v) 6 μl of tri-Sodium citrate (1.6 M) 333 μl of water (100 v/v) |
H7 |
| NA | 250 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 710 μl of water (100 v/v) |
H8 |
| NA | 500 μl of Lithium sulfate (2 M) 40 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v) |
H9 |
| NA | 20 μl of Lithium chloride (10 M) 25 μl of Magnesium sulfate (2 M) 160 μl of Polyethylene glycol 8,000 (50 w/v) 795 μl of water (100 v/v) |
H10 |
| pH 6.5 | 100 μl of Imidazole pH 6.5 (1 M) 100 μl of Zinc acetate (2 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v) |
H11 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 200 μl of Calcium acetate (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 500 μl of water (100 v/v) |
H12 |
| NA | 200 μl of Magnesium acetate (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v) |