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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
c3_5
Barcodes
c3_5
Screen Structure
c3_5
Well Count | 96 |
---|---|
Number of distinct ingredients | 46 |
Internal diversity (score mean dist) | 0.877 |
Screen Composition
ingredient type | c3_5 |
---|---|
Buffer | 91 |
Cryoprotectant | 8 |
Detergent | 2 |
Organic (non-volatile) | 14 |
Organic (volatile) | 7 |
Polymer | 7 |
Salt | 138 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 6.5 | 214.3 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of magnesium sulfate BCC (2.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 605.7 μl of water (100 v/v) |
A2 |
| NA | 285.7 μl of ammonium sulfate BCC (3.5 M) 20.0 μl of copper(II) sulfate BCC (1 M) 694.3 μl of water (100 v/v) |
A3 |
| NA | 285.7 μl of ammonium sulfate BCC (3.5 M) 333.3 μl of potassium chloride BCC (3 M) 381.0 μl of water (100 v/v) |
A4 |
| pH 5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of lithium sulfate BCC (2.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 120.0 μl of trehalose BCC (1 M) 414.3 μl of water (100 v/v) |
A5 |
| pH 5.5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 120.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 30.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 564.3 μl of water (100 v/v) |
A6 |
| pH 5.5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 50.0 μl of polyethylene glycol 3350 BCC (20 w/v) 564.3 μl of water (100 v/v) |
A7 |
| pH 5.5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 40.0 μl of sodium chloride BCC (5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 574.3 μl of water (100 v/v) |
A8 |
| pH 7 | 285.7 μl of ammonium sulfate BCC (3.5 M) 25.0 μl of polyethylene glycol 8000 BCC (20 w/v) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 589.3 μl of water (100 v/v) |
A9 |
| pH 7 | 285.7 μl of ammonium sulfate BCC (3.5 M) 400.0 μl of lithium sulfate BCC (2.5 M) 81.7 μl of sodium HEPES BCC pH 6.5 (1 M) 18.3 μl of sodium HEPES BCC pH 8.5 (1 M) 75.0 μl of trehalose BCC (1 M) 139.3 μl of water (100 v/v) |
A10 |
| pH 8.5 | 285.7 μl of ammonium sulfate BCC (3.5 M) 400.0 μl of lithium sulfate BCC (2.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 214.3 μl of water (100 v/v) |
A11 |
| pH 5 | 360.0 μl of ammonium sulfate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 40.0 μl of sodium chloride BCC (5 M) 500.0 μl of water (100 v/v) |
A12 |
| pH 6 | 360.0 μl of ammonium sulfate BCC (3.5 M) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 540.0 μl of water (100 v/v) |
B1 |
| pH 6.5 | 360.0 μl of ammonium sulfate BCC (3.5 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 540.0 μl of water (100 v/v) |
B2 |
| pH 7.5 | 360.0 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 540.0 μl of water (100 v/v) |
B3 |
| pH 8.5 | 360.0 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of lithium sulfate BCC (2.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 460.0 μl of water (100 v/v) |
B4 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 360.0 μl of ammonium sulfate BCC (3.5 M) 40.0 μl of sodium chloride BCC (5 M) 475.0 μl of water (100 v/v) |
B5 |
| NA | 428.6 μl of ammonium sulfate BCC (3.5 M) 10.0 μl of tergitol NP-40 (1 w/v) 561.4 μl of water (100 v/v) |
B6 |
| NA | 25.0 μl of 1-octanol BCC (100 v/v) 428.6 μl of ammonium sulfate BCC (3.5 M) 546.4 μl of water (100 v/v) |
B7 |
| NA | 428.6 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 50.0 μl of magnesium chloride BCC (1 M) 16.7 μl of potassium chloride BCC (3 M) 379.8 μl of water (100 v/v) |
B8 |
| pH 5.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 40.0 μl of sodium chloride BCC (5 M) 120.0 μl of trehalose BCC (1 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 311.4 μl of water (100 v/v) |
B9 |
| pH 5.5 | 34.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 66.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 428.6 μl of ammonium sulfate BCC (3.5 M) 471.4 μl of water (100 v/v) |
B10 |
| pH 5.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 120.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 30.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 421.4 μl of water (100 v/v) |
B11 |
| pH 6 | 428.6 μl of ammonium sulfate BCC (3.5 M) 34.6 μl of sodium cacodylate BCC pH 5.3 (1 M) 15.4 μl of sodium cacodylate BCC pH 7.3 (1 M) 521.4 μl of water (100 v/v) |
B12 |
| pH 6.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 20.0 μl of sodium chloride BCC (5 M) 451.4 μl of water (100 v/v) |
C1 |
| pH 7.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 25.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 120.0 μl of trehalose BCC (1 M) 326.4 μl of water (100 v/v) |
C2 |
| pH 8.5 | 428.6 μl of ammonium sulfate BCC (3.5 M) 150.0 μl of glycerol BCC (80 v/v) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 321.4 μl of water (100 v/v) |
C3 |
| pH 6.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 442.9 μl of water (100 v/v) |
C4 |
| pH 6.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of dioxane BCC (100 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 342.9 μl of water (100 v/v) |
C5 |
| pH 7.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of dioxane BCC (100 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 342.9 μl of water (100 v/v) |
C6 |
| pH 7.5 | 457.1 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 20.0 μl of sodium chloride BCC (5 M) 422.9 μl of water (100 v/v) |
C7 |
| pH 6.5 | 514.3 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of cobalt chloride BCC (0.1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 285.7 μl of water (100 v/v) |
C8 |
| NA | 62.5 μl of 2-propanol BCC (80 v/v) 571.4 μl of ammonium sulfate BCC (3.5 M) 366.1 μl of water (100 v/v) |
C9 |
| NA | 571.4 μl of ammonium sulfate BCC (3.5 M) 428.6 μl of water (100 v/v) |
C10 |
| NA | 571.4 μl of ammonium sulfate BCC (3.5 M) 200.0 μl of sodium chloride BCC (5 M) 228.6 μl of water (100 v/v) |
C11 |
| NA | 571.4 μl of ammonium sulfate BCC (3.5 M) 200.0 μl of ethylene glycol BCC (100 v/v) 80.0 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v) 148.6 μl of water (100 v/v) |
C12 |
| pH 3.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 328.6 μl of water (100 v/v) |
D1 |
| pH 4.2 | 571.4 μl of ammonium sulfate BCC (3.5 M) 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 228.6 μl of water (100 v/v) |
D2 |
| pH 5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 328.6 μl of water (100 v/v) |
D3 |
| pH 5.5 | 34.0 μl of DL-malate-imidazole BCC pH 2 (1 M) 66.0 μl of DL-malate-imidazole BCC pH 9 (1 M) 571.4 μl of ammonium sulfate BCC (3.5 M) 328.6 μl of water (100 v/v) |
D4 |
| pH 5.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 328.6 μl of water (100 v/v) |
D5 |
| pH 5.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 40.0 μl of sodium chloride BCC (5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 288.6 μl of water (100 v/v) |
D6 |
| pH 5.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 328.6 μl of water (100 v/v) |
D7 |
| pH 5.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of potassium sodium tartrate BCC (2 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 228.6 μl of water (100 v/v) |
D8 |
| pH 6.5 | 109.4 μl of ADA BCC pH 5.5 (0.5 M) 90.6 μl of ADA BCC pH 7.5 (0.5 M) 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of ethanol BCC (100 v/v) 128.6 μl of water (100 v/v) |
D9 |
| pH 6.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 40.0 μl of sodium chloride BCC (5 M) 288.6 μl of water (100 v/v) |
D10 |
| pH 6.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 328.6 μl of water (100 v/v) |
D11 |
| pH 6.5 | 109.4 μl of ADA BCC pH 5.5 (0.5 M) 90.6 μl of ADA BCC pH 7.5 (0.5 M) 571.4 μl of ammonium sulfate BCC (3.5 M) 40.0 μl of magnesium sulfate BCC (2.5 M) 188.6 μl of water (100 v/v) |
D12 |
| pH 7 | 571.4 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of lithium sulfate BCC (2.5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 248.6 μl of water (100 v/v) |
E1 |
| pH 7.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v) |
E2 |
| pH 7.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 25.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 303.6 μl of water (100 v/v) |
E3 |
| pH 8.5 | 571.4 μl of ammonium sulfate BCC (3.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 328.6 μl of water (100 v/v) |
E4 |
| pH 9.4 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 571.4 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of sodium bicine BCC pH 9.4 (1 M) 278.6 μl of water (100 v/v) |
E5 |
| pH 9.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 571.4 μl of ammonium sulfate BCC (3.5 M) 253.6 μl of water (100 v/v) |
E6 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 571.4 μl of ammonium sulfate BCC (3.5 M) 80.0 μl of lithium sulfate BCC (2.5 M) 148.6 μl of water (100 v/v) |
E7 |
| NA | 700.0 μl of ammonium sulfate BCC (3.5 M) 100.0 μl of dimethyl sulfoxide BCC (100 v/v) 200.0 μl of water (100 v/v) |
E8 |
| NA | 700.0 μl of ammonium sulfate BCC (3.5 M) 125.0 μl of glycerol BCC (80 v/v) 175.0 μl of water (100 v/v) |
E9 |
| pH 6.5 | 714.3 μl of ammonium sulfate BCC (3.5 M) 60.0 μl of ethanol BCC (100 v/v) 13.9 μl of sodium MES BCC pH 5.2 (1 M) 36.1 μl of sodium MES BCC pH 7.2 (1 M) 175.7 μl of water (100 v/v) |
E10 |
| pH 7 | 771.4 μl of ammonium sulfate BCC (3.5 M) 63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M) 36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M) 128.6 μl of water (100 v/v) |
E11 |
| pH 8.5 | 771.4 μl of ammonium sulfate BCC (3.5 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 178.6 μl of water (100 v/v) |
E12 |
| pH 9.5 | 39.9 μl of CHES BCC pH 10 (0.8 M) 22.6 μl of CHES BCC pH 8.3 (0.8 M) 771.4 μl of ammonium sulfate BCC (3.5 M) 166.1 μl of water (100 v/v) |
F1 |
| NA | 285.7 μl of ammonium sulfate BCC (3.5 M) 400.0 μl of cesium chloride BCC (5 M) 314.3 μl of water (100 v/v) |
F2 |
| pH 7.5 | 100.0 μl of cadmium sulfate BCC (0.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 333.3 μl of sodium acetate BCC (3 M) 466.7 μl of water (100 v/v) |
F3 |
| NA | 750.0 μl of cobalt(II) sulfate BCC (2 M) 250.0 μl of water (100 v/v) |
F4 |
| pH 8.5 | 80.0 μl of lithium sulfate BCC (2.5 M) 400.0 μl of sodium chloride BCC (5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 420.0 μl of water (100 v/v) |
F5 |
| pH 6 | 200.0 μl of lithium sulfate BCC (2.5 M) 69.1 μl of sodium cacodylate BCC pH 5.3 (1 M) 30.9 μl of sodium cacodylate BCC pH 7.3 (1 M) 120.0 μl of trehalose BCC (1 M) 580.0 μl of water (100 v/v) |
F6 |
| pH 4.5 | 320.0 μl of lithium sulfate BCC (2.5 M) 15.6 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.4 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 630.0 μl of water (100 v/v) |
F7 |
| NA | 400.0 μl of lithium sulfate BCC (2.5 M) 100.0 μl of polyethylene glycol 8000 BCC (20 w/v) 500.0 μl of water (100 v/v) |
F8 |
| pH 4.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of water (100 v/v) |
F9 |
| pH 5.5 | 142.9 μl of ammonium sulfate BCC (3.5 M) 400.0 μl of lithium sulfate BCC (2.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 357.1 μl of water (100 v/v) |
F10 |
| pH 6.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 25.0 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 475.0 μl of water (100 v/v) |
F11 |
| pH 8.5 | 400.0 μl of lithium sulfate BCC (2.5 M) 10.0 μl of nickel(II) chloride BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 490.0 μl of water (100 v/v) |
F12 |
| pH 8 | 520.0 μl of lithium sulfate BCC (2.5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 380.0 μl of water (100 v/v) |
G1 |
| pH 7.5 | 600.0 μl of lithium sulfate BCC (2.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G2 |
| pH 7 | 720.0 μl of lithium sulfate BCC (2.5 M) 63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M) 36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M) 180.0 μl of water (100 v/v) |
G3 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 800.0 μl of lithium sulfate BCC (2.5 M) 75.0 μl of water (100 v/v) |
G4 |
| pH 5.5 | 300.0 μl of magnesium sulfate BCC (2.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 600.0 μl of water (100 v/v) |
G5 |
| pH 6 | 400.0 μl of magnesium sulfate BCC (2.5 M) 61.6 μl of sodium MES BCC pH 5.2 (1 M) 38.4 μl of sodium MES BCC pH 7.2 (1 M) 120.0 μl of trehalose BCC (1 M) 380.0 μl of water (100 v/v) |
G6 |
| pH 5.5 | 600.0 μl of magnesium sulfate BCC (2.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 300.0 μl of water (100 v/v) |
G7 |
| pH 6.5 | 640.0 μl of magnesium sulfate BCC (2.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 260.0 μl of water (100 v/v) |
G8 |
| pH 7.5 | 640.0 μl of magnesium sulfate BCC (2.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 120.0 μl of trehalose BCC (1 M) 140.0 μl of water (100 v/v) |
G9 |
| NA | 187.5 μl of glycerol BCC (80 v/v) 800.0 μl of magnesium sulfate BCC (2.5 M) 12.5 μl of water (100 v/v) |
G10 |
| pH 8.5 | 800.0 μl of magnesium sulfate BCC (2.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
G11 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 800.0 μl of magnesium sulfate BCC (2.5 M) 75.0 μl of water (100 v/v) |
G12 |
| NA | 720.0 μl of triammonium citrate BCC (2.5 M) 280.0 μl of water (100 v/v) |
H1 |
| pH 8.5 | 10.0 μl of sodium tetraborate BCC pH 8.5 (1 M) 468.8 μl of trisodium citrate BCC (1.6 M) 521.2 μl of water (100 v/v) |
H2 |
| pH 6.5 | 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 275.0 μl of water (100 v/v) |
H3 |
| pH 6.5 | 62.5 μl of polyethylene glycol 400 BCC (80 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 212.5 μl of water (100 v/v) |
H4 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 275.0 μl of water (100 v/v) |
H5 |
| pH 8.5 | 10.0 μl of sodium tetraborate BCC pH 8.5 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 365.0 μl of water (100 v/v) |
H6 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 625.0 μl of trisodium citrate BCC (1.6 M) 250.0 μl of water (100 v/v) |
H7 |
| pH 6 | 41.5 μl of sodium cacodylate BCC pH 5.3 (1 M) 18.5 μl of sodium cacodylate BCC pH 7.3 (1 M) 875.0 μl of trisodium citrate BCC (1.6 M) 65.0 μl of water (100 v/v) |
H8 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 875.0 μl of trisodium citrate BCC (1.6 M) 25.0 μl of water (100 v/v) |
H9 |
| pH 8.5 | 12.7 μl of tris chloride BCC pH 7 (1 M) 47.3 μl of tris chloride BCC pH 9 (1 M) 875.0 μl of trisodium citrate BCC (1.6 M) 65.0 μl of water (100 v/v) |
H10 |
| pH 8.5 | 10.0 μl of sodium tetraborate BCC pH 8.5 (1 M) 937.5 μl of trisodium citrate BCC (1.6 M) 52.5 μl of water (100 v/v) |
H11 |
| NA | 750.0 μl of trisodium citrate BCC (1.6 M) 250.0 μl of water (100 v/v) |
H12 |
| NA | 1000.0 μl of trisodium citrate BCC (1.6 M) |