Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
c3_5
Barcodes
c3_5
- Z0002712
- Z0002713
- Z0002644
Screen Structure
c3_5
Well Count | 96 |
---|---|
Number of distinct ingredients | 46 |
Internal diversity (score mean dist) | 0.877 |
Screen Composition
ingredient type | c3_5 |
---|---|
Buffer | 91 |
Cryoprotectant | 8 |
Detergent | 2 |
Organic (non-volatile) | 14 |
Organic (volatile) | 7 |
Polymer | 7 |
Salt | 138 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 6.5 | 214 μl of ammonium sulfate BCC (3.5 M) 80 μl of magnesium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 605 μl of water (100 v/v) |
A2 |
| NA | 285 μl of ammonium sulfate BCC (3.5 M) 20 μl of copper(II) sulfate BCC (1 M) 694 μl of water (100 v/v) |
A3 |
| NA | 285 μl of ammonium sulfate BCC (3.5 M) 333 μl of potassium chloride BCC (3 M) 381 μl of water (100 v/v) |
A4 |
| pH 5 | 285 μl of ammonium sulfate BCC (3.5 M) 80 μl of lithium sulfate BCC (2.5 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 120 μl of trehalose BCC (1 M) 414 μl of water (100 v/v) |
A5 |
| pH 5.5 | 285 μl of ammonium sulfate BCC (3.5 M) 30 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 120 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 564 μl of water (100 v/v) |
A6 |
| pH 5.5 | 285 μl of ammonium sulfate BCC (3.5 M) 50 μl of polyethylene glycol 3350 BCC (20 w/v) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 564 μl of water (100 v/v) |
A7 |
| pH 5.5 | 285 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 40 μl of sodium chloride BCC (5 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 574 μl of water (100 v/v) |
A8 |
| pH 7 | 285 μl of ammonium sulfate BCC (3.5 M) 25 μl of polyethylene glycol 8000 BCC (20 w/v) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 589 μl of water (100 v/v) |
A9 |
| pH 7 | 285 μl of ammonium sulfate BCC (3.5 M) 400 μl of lithium sulfate BCC (2.5 M) 81 μl of sodium HEPES BCC pH 6.5 (1 M) 18 μl of sodium HEPES BCC pH 8.5 (1 M) 75 μl of trehalose BCC (1 M) 139 μl of water (100 v/v) |
A10 |
| pH 8.5 | 285 μl of ammonium sulfate BCC (3.5 M) 400 μl of lithium sulfate BCC (2.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 214 μl of water (100 v/v) |
A11 |
| pH 5 | 360 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 40 μl of sodium chloride BCC (5 M) 500 μl of water (100 v/v) |
A12 |
| pH 6 | 360 μl of ammonium sulfate BCC (3.5 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 540 μl of water (100 v/v) |
B1 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 360 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 540 μl of water (100 v/v) |
B2 |
| pH 7.5 | 360 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 540 μl of water (100 v/v) |
B3 |
| pH 8.5 | 360 μl of ammonium sulfate BCC (3.5 M) 80 μl of lithium sulfate BCC (2.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 460 μl of water (100 v/v) |
B4 |
| pH 9.5 | 360 μl of ammonium sulfate BCC (3.5 M) 40 μl of sodium chloride BCC (5 M) 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 475 μl of water (100 v/v) |
B5 |
| NA | 428 μl of ammonium sulfate BCC (3.5 M) 10 μl of tergitol NP-40 (1 w/v) 561 μl of water (100 v/v) |
B6 |
| NA | 428 μl of ammonium sulfate BCC (3.5 M) 25 μl of 1-octanol BCC (100 v/v) 546 μl of water (100 v/v) |
B7 |
| NA | 428 μl of ammonium sulfate BCC (3.5 M) 16 μl of potassium chloride BCC (3 M) 125 μl of glycerol BCC (80 v/v) 50 μl of magnesium chloride BCC (1 M) 379 μl of water (100 v/v) |
B8 |
| pH 5.5 | 428 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 40 μl of sodium chloride BCC (5 M) 120 μl of trehalose BCC (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 311 μl of water (100 v/v) |
B9 |
| pH 5.5 | 428 μl of ammonium sulfate BCC (3.5 M) 34 μl of DL-malate-imidazole BCC pH 2 (1 M) 66 μl of DL-malate-imidazole BCC pH 9 (1 M) 471 μl of water (100 v/v) |
B10 |
| pH 5.5 | 428 μl of ammonium sulfate BCC (3.5 M) 30 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 120 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 421 μl of water (100 v/v) |
B11 |
| pH 6 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 428 μl of ammonium sulfate BCC (3.5 M) 15 μl of sodium cacodylate BCC pH 7.3 (1 M) 521 μl of water (100 v/v) |
B12 |
| pH 6.5 | 428 μl of ammonium sulfate BCC (3.5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 20 μl of sodium chloride BCC (5 M) 451 μl of water (100 v/v) |
C1 |
| pH 7.5 | 428 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 25 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 120 μl of trehalose BCC (1 M) 326 μl of water (100 v/v) |
C2 |
| pH 8.5 | 428 μl of ammonium sulfate BCC (3.5 M) 150 μl of glycerol BCC (80 v/v) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 321 μl of water (100 v/v) |
C3 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 457 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 442 μl of water (100 v/v) |
C4 |
| pH 6.5 | 457 μl of ammonium sulfate BCC (3.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 100 μl of dioxane BCC (100 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 342 μl of water (100 v/v) |
C5 |
| pH 7.5 | 457 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 100 μl of dioxane BCC (100 v/v) 342 μl of water (100 v/v) |
C6 |
| pH 7.5 | 457 μl of ammonium sulfate BCC (3.5 M) 20 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 422 μl of water (100 v/v) |
C7 |
| pH 6.5 | 514 μl of ammonium sulfate BCC (3.5 M) 100 μl of cobalt chloride BCC (0.1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 285 μl of water (100 v/v) |
C8 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 62 μl of 2-propanol BCC (80 v/v) 366 μl of water (100 v/v) |
C9 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 428 μl of water (100 v/v) |
C10 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 200 μl of sodium chloride BCC (5 M) 228 μl of water (100 v/v) |
C11 |
| NA | 571 μl of ammonium sulfate BCC (3.5 M) 80 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v) 200 μl of ethylene glycol BCC (100 v/v) 148 μl of water (100 v/v) |
C12 |
| pH 3.5 | 571 μl of ammonium sulfate BCC (3.5 M) 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 328 μl of water (100 v/v) |
D1 |
| pH 4.2 | 571 μl of ammonium sulfate BCC (3.5 M) 112 μl of phosphate-citrate BCC pH 9.3 (0.5 M) 88 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 228 μl of water (100 v/v) |
D2 |
| pH 5 | 571 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 328 μl of water (100 v/v) |
D3 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 34 μl of DL-malate-imidazole BCC pH 2 (1 M) 66 μl of DL-malate-imidazole BCC pH 9 (1 M) 328 μl of water (100 v/v) |
D4 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 328 μl of water (100 v/v) |
D5 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 40 μl of sodium chloride BCC (5 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 288 μl of water (100 v/v) |
D6 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 328 μl of water (100 v/v) |
D7 |
| pH 5.5 | 571 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 100 μl of potassium sodium tartrate BCC (2 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 228 μl of water (100 v/v) |
D8 |
| pH 6.5 | 571 μl of ammonium sulfate BCC (3.5 M) 109 μl of ADA BCC pH 5.5 (0.5 M) 100 μl of ethanol BCC (100 v/v) 90 μl of ADA BCC pH 7.5 (0.5 M) 128 μl of water (100 v/v) |
D9 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 571 μl of ammonium sulfate BCC (3.5 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 40 μl of sodium chloride BCC (5 M) 288 μl of water (100 v/v) |
D10 |
| pH 6.5 | 571 μl of ammonium sulfate BCC (3.5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 328 μl of water (100 v/v) |
D11 |
| pH 6.5 | 571 μl of ammonium sulfate BCC (3.5 M) 40 μl of magnesium sulfate BCC (2.5 M) 109 μl of ADA BCC pH 5.5 (0.5 M) 90 μl of ADA BCC pH 7.5 (0.5 M) 188 μl of water (100 v/v) |
D12 |
| pH 7 | 571 μl of ammonium sulfate BCC (3.5 M) 80 μl of lithium sulfate BCC (2.5 M) 100 μl of tris chloride BCC pH 7 (1 M) 248 μl of water (100 v/v) |
E1 |
| pH 7.5 | 571 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 328 μl of water (100 v/v) |
E2 |
| pH 7.5 | 571 μl of ammonium sulfate BCC (3.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 25 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 303 μl of water (100 v/v) |
E3 |
| pH 8.5 | 571 μl of ammonium sulfate BCC (3.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 328 μl of water (100 v/v) |
E4 |
| pH 9.4 | 571 μl of ammonium sulfate BCC (3.5 M) 100 μl of sodium bicine BCC pH 9.4 (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 278 μl of water (100 v/v) |
E5 |
| pH 9.5 | 571 μl of ammonium sulfate BCC (3.5 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 253 μl of water (100 v/v) |
E6 |
| pH 10 | 571 μl of ammonium sulfate BCC (3.5 M) 114 μl of CAPS BCC pH 11 (0.5 M) 80 μl of lithium sulfate BCC (2.5 M) 86 μl of CAPS BCC pH 9.4 (0.5 M) 148 μl of water (100 v/v) |
E7 |
| NA | 700 μl of ammonium sulfate BCC (3.5 M) 100 μl of dimethyl sulfoxide BCC (100 v/v) 200 μl of water (100 v/v) |
E8 |
| NA | 700 μl of ammonium sulfate BCC (3.5 M) 125 μl of glycerol BCC (80 v/v) 175 μl of water (100 v/v) |
E9 |
| pH 6.5 | 714 μl of ammonium sulfate BCC (3.5 M) 60 μl of ethanol BCC (100 v/v) 36 μl of sodium MES BCC pH 7.2 (1 M) 13 μl of sodium MES BCC pH 5.2 (1 M) 175 μl of water (100 v/v) |
E10 |
| pH 7 | 771 μl of ammonium sulfate BCC (3.5 M) 36 μl of sodium MOPS BCC pH 8.2 (0.5 M) 63 μl of sodium MOPS BCC pH 6.2 (0.5 M) 128 μl of water (100 v/v) |
E11 |
| pH 8.5 | 771 μl of ammonium sulfate BCC (3.5 M) 39 μl of tris chloride BCC pH 9 (1 M) 10 μl of tris chloride BCC pH 7 (1 M) 178 μl of water (100 v/v) |
E12 |
| pH 9.5 | 771 μl of ammonium sulfate BCC (3.5 M) 39 μl of CHES BCC pH 10 (0.8 M) 22 μl of CHES BCC pH 8.3 (0.8 M) 166 μl of water (100 v/v) |
F1 |
| NA | 285 μl of ammonium sulfate BCC (3.5 M) 400 μl of cesium chloride BCC (5 M) 314 μl of water (100 v/v) |
F2 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 333 μl of sodium acetate BCC (3 M) 100 μl of cadmium sulfate BCC (0.5 M) 466 μl of water (100 v/v) |
F3 |
| NA | 750 μl of cobalt(II) sulfate BCC (2 M) 250 μl of water (100 v/v) |
F4 |
| pH 8.5 | 80 μl of lithium sulfate BCC (2.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 400 μl of sodium chloride BCC (5 M) 420 μl of water (100 v/v) |
F5 |
| pH 6 | 69 μl of sodium cacodylate BCC pH 5.3 (1 M) 200 μl of lithium sulfate BCC (2.5 M) 30 μl of sodium cacodylate BCC pH 7.3 (1 M) 120 μl of trehalose BCC (1 M) 580 μl of water (100 v/v) |
F6 |
| pH 4.5 | 320 μl of lithium sulfate BCC (2.5 M) 15 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 630 μl of water (100 v/v) |
F7 |
| NA | 100 μl of polyethylene glycol 8000 BCC (20 w/v) 400 μl of lithium sulfate BCC (2.5 M) 500 μl of water (100 v/v) |
F8 |
| pH 4.5 | 400 μl of lithium sulfate BCC (2.5 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500 μl of water (100 v/v) |
F9 |
| pH 5.5 | 142 μl of ammonium sulfate BCC (3.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 400 μl of lithium sulfate BCC (2.5 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 357 μl of water (100 v/v) |
F10 |
| pH 6.5 | 400 μl of lithium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 25 μl of polyethylene glycol 400 BCC (80 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 475 μl of water (100 v/v) |
F11 |
| pH 8.5 | 400 μl of lithium sulfate BCC (2.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 10 μl of nickel(II) chloride BCC (1 M) 490 μl of water (100 v/v) |
F12 |
| pH 8 | 520 μl of lithium sulfate BCC (2.5 M) 45 μl of tris chloride BCC pH 9 (1 M) 54 μl of tris chloride BCC pH 7 (1 M) 380 μl of water (100 v/v) |
G1 |
| pH 7.5 | 600 μl of lithium sulfate BCC (2.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 300 μl of water (100 v/v) |
G2 |
| pH 7 | 36 μl of sodium MOPS BCC pH 8.2 (0.5 M) 720 μl of lithium sulfate BCC (2.5 M) 63 μl of sodium MOPS BCC pH 6.2 (0.5 M) 180 μl of water (100 v/v) |
G3 |
| pH 9.5 | 800 μl of lithium sulfate BCC (2.5 M) 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 75 μl of water (100 v/v) |
G4 |
| pH 5.5 | 300 μl of magnesium sulfate BCC (2.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 600 μl of water (100 v/v) |
G5 |
| pH 6 | 400 μl of magnesium sulfate BCC (2.5 M) 38 μl of sodium MES BCC pH 7.2 (1 M) 120 μl of trehalose BCC (1 M) 61 μl of sodium MES BCC pH 5.2 (1 M) 380 μl of water (100 v/v) |
G6 |
| pH 5.5 | 600 μl of magnesium sulfate BCC (2.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 300 μl of water (100 v/v) |
G7 |
| pH 6.5 | 640 μl of magnesium sulfate BCC (2.5 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 260 μl of water (100 v/v) |
G8 |
| pH 7.5 | 640 μl of magnesium sulfate BCC (2.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 120 μl of trehalose BCC (1 M) 140 μl of water (100 v/v) |
G9 |
| NA | 800 μl of magnesium sulfate BCC (2.5 M) 187 μl of glycerol BCC (80 v/v) 12 μl of water (100 v/v) |
G10 |
| pH 8.5 | 800 μl of magnesium sulfate BCC (2.5 M) 78 μl of tris chloride BCC pH 9 (1 M) 21 μl of tris chloride BCC pH 7 (1 M) 100 μl of water (100 v/v) |
G11 |
| pH 9.5 | 800 μl of magnesium sulfate BCC (2.5 M) 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 75 μl of water (100 v/v) |
G12 |
| NA | 720 μl of triammonium citrate BCC (2.5 M) 280 μl of water (100 v/v) |
H1 |
| pH 8.5 | 10 μl of sodium tetraborate BCC pH 8.5 (1 M) 468 μl of trisodium citrate BCC (1.6 M) 521 μl of water (100 v/v) |
H2 |
| pH 6.5 | 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 625 μl of trisodium citrate BCC (1.6 M) 275 μl of water (100 v/v) |
H3 |
| pH 6.5 | 72 μl of sodium MES BCC pH 7.2 (1 M) 625 μl of trisodium citrate BCC (1.6 M) 62 μl of polyethylene glycol 400 BCC (80 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 212 μl of water (100 v/v) |
H4 |
| pH 8 | 100 μl of imidazole BCC pH 8 (1 M) 625 μl of trisodium citrate BCC (1.6 M) 275 μl of water (100 v/v) |
H5 |
| pH 8.5 | 10 μl of sodium tetraborate BCC pH 8.5 (1 M) 625 μl of trisodium citrate BCC (1.6 M) 365 μl of water (100 v/v) |
H6 |
| pH 9.5 | 625 μl of trisodium citrate BCC (1.6 M) 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 250 μl of water (100 v/v) |
H7 |
| pH 6 | 41 μl of sodium cacodylate BCC pH 5.3 (1 M) 18 μl of sodium cacodylate BCC pH 7.3 (1 M) 875 μl of trisodium citrate BCC (1.6 M) 65 μl of water (100 v/v) |
H8 |
| pH 7.5 | 875 μl of trisodium citrate BCC (1.6 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 25 μl of water (100 v/v) |
H9 |
| pH 8.5 | 47 μl of tris chloride BCC pH 9 (1 M) 12 μl of tris chloride BCC pH 7 (1 M) 875 μl of trisodium citrate BCC (1.6 M) 65 μl of water (100 v/v) |
H10 |
| pH 8.5 | 10 μl of sodium tetraborate BCC pH 8.5 (1 M) 937 μl of trisodium citrate BCC (1.6 M) 52 μl of water (100 v/v) |
H11 |
| NA | 750 μl of trisodium citrate BCC (1.6 M) 250 μl of water (100 v/v) |
H12 |
| NA | 1000 μl of trisodium citrate BCC (1.6 M) |