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Supplemental Information

Screen Description

c3_5

Created 2025-03-07.
Salty: ammonium sulfate, other sulfates, citrates

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Barcodes

c3_5

  • Z0002712
  • Z0002713
  • Z0002644

Screen Structure

c3_5

Well Count 96
Number of distinct ingredients 46
Internal diversity (score mean dist) 0.877

Screen Composition

2025-06-13T21:31:49.085055 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
ingredient type c3_5
Buffer 91
Cryoprotectant 8
Detergent 2
Organic (non-volatile) 14
Organic (volatile) 7
Polymer 7
Salt 138

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_5 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 0.75 M -
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 0.2 M -
pH 6.5
214 μl of ammonium sulfate BCC (3.5 M)
80 μl of magnesium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
605 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1 M -
  • copper(II) sulfate BCC 0.02 M -
NA
285 μl of ammonium sulfate BCC (3.5 M)
20 μl of copper(II) sulfate BCC (1 M)
694 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 1 M -
  • potassium chloride BCC 1 M -
NA
285 μl of ammonium sulfate BCC (3.5 M)
333 μl of potassium chloride BCC (3 M)
381 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 0.2 M -
  • trehalose BCC 0.12 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
285 μl of ammonium sulfate BCC (3.5 M)
80 μl of lithium sulfate BCC (2.5 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
120 μl of trehalose BCC (1 M)
414 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.15 M pH 5.5
pH 5.5
285 μl of ammonium sulfate BCC (3.5 M)
30 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
120 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
564 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 1 M -
  • polyethylene glycol 3350 BCC 1 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
285 μl of ammonium sulfate BCC (3.5 M)
50 μl of polyethylene glycol 3350 BCC (20 w/v)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
564 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
285 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
40 μl of sodium chloride BCC (5 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
574 μl of water (100 v/v)
A8
  • ammonium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 0.5 w/v -
pH 7
285 μl of ammonium sulfate BCC (3.5 M)
25 μl of polyethylene glycol 8000 BCC (20 w/v)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
589 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • trehalose BCC 0.075 M -
pH 7
285 μl of ammonium sulfate BCC (3.5 M)
400 μl of lithium sulfate BCC (2.5 M)
81 μl of sodium HEPES BCC pH 6.5 (1 M)
18 μl of sodium HEPES BCC pH 8.5 (1 M)
75 μl of trehalose BCC (1 M)
139 μl of water (100 v/v)
A10
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
285 μl of ammonium sulfate BCC (3.5 M)
400 μl of lithium sulfate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
214 μl of water (100 v/v)
A11
  • ammonium sulfate BCC 1.26 M -
  • sodium chloride BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
360 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
40 μl of sodium chloride BCC (5 M)
500 μl of water (100 v/v)
A12
  • ammonium sulfate BCC 1.26 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
360 μl of ammonium sulfate BCC (3.5 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
540 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 1.26 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
360 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
540 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1.26 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
360 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
540 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.26 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
360 μl of ammonium sulfate BCC (3.5 M)
80 μl of lithium sulfate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
460 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 1.26 M -
  • CHES BCC 0.1 M pH 9.5
  • sodium chloride BCC 0.2 M -
pH 9.5
360 μl of ammonium sulfate BCC (3.5 M)
40 μl of sodium chloride BCC (5 M)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
475 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 1.5 M -
  • tergitol NP-40 0.01 w/v -
NA
428 μl of ammonium sulfate BCC (3.5 M)
10 μl of tergitol NP-40 (1 w/v)
561 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 1.5 M -
  • 1-octanol BCC 2.5 v/v -
NA
428 μl of ammonium sulfate BCC (3.5 M)
25 μl of 1-octanol BCC (100 v/v)
546 μl of water (100 v/v)
B7
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 10 v/v -
  • magnesium chloride BCC 0.05 M -
  • potassium chloride BCC 0.05 M -
NA
428 μl of ammonium sulfate BCC (3.5 M)
16 μl of potassium chloride BCC (3 M)
125 μl of glycerol BCC (80 v/v)
50 μl of magnesium chloride BCC (1 M)
379 μl of water (100 v/v)
B8
  • ammonium sulfate BCC 1.5 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • trehalose BCC 0.12 M -
pH 5.5
428 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
40 μl of sodium chloride BCC (5 M)
120 μl of trehalose BCC (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
311 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-imidazole BCC 0.1 M pH 5.5
pH 5.5
428 μl of ammonium sulfate BCC (3.5 M)
34 μl of DL-malate-imidazole BCC pH 2 (1 M)
66 μl of DL-malate-imidazole BCC pH 9 (1 M)
471 μl of water (100 v/v)
B10
  • ammonium sulfate BCC 1.5 M -
  • trisodium citrate-citric acid BCC 0.15 M pH 5.5
pH 5.5
428 μl of ammonium sulfate BCC (3.5 M)
30 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
120 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
421 μl of water (100 v/v)
B11
  • ammonium sulfate BCC 1.5 M -
  • sodium cacodylate BCC 0.05 M pH 6
pH 6
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
428 μl of ammonium sulfate BCC (3.5 M)
15 μl of sodium cacodylate BCC pH 7.3 (1 M)
521 μl of water (100 v/v)
B12
  • ammonium sulfate BCC 1.5 M -
  • sodium chloride BCC 0.1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
428 μl of ammonium sulfate BCC (3.5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
20 μl of sodium chloride BCC (5 M)
451 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 1.5 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • trehalose BCC 0.12 M -
pH 7.5
428 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
120 μl of trehalose BCC (1 M)
326 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 12 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428 μl of ammonium sulfate BCC (3.5 M)
150 μl of glycerol BCC (80 v/v)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
321 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.6 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
457 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
442 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
457 μl of ammonium sulfate BCC (3.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
100 μl of dioxane BCC (100 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
342 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
100 μl of dioxane BCC (100 v/v)
342 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 1.6 M -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457 μl of ammonium sulfate BCC (3.5 M)
20 μl of sodium chloride BCC (5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
422 μl of water (100 v/v)
C7
  • ammonium sulfate BCC 1.8 M -
  • cobalt chloride BCC 0.01 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
514 μl of ammonium sulfate BCC (3.5 M)
100 μl of cobalt chloride BCC (0.1 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
285 μl of water (100 v/v)
C8
  • 2-propanol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
62 μl of 2-propanol BCC (80 v/v)
366 μl of water (100 v/v)
C9
  • ammonium sulfate BCC 2 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
428 μl of water (100 v/v)
C10
  • ammonium sulfate BCC 2 M -
  • sodium chloride BCC 1 M -
NA
571 μl of ammonium sulfate BCC (3.5 M)
200 μl of sodium chloride BCC (5 M)
228 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 2 M -
  • ethylene glycol BCC 20 v/v -
  • n-octyl-b-D-Glucopyranoside BCC 0.8 w/v -
NA
571 μl of ammonium sulfate BCC (3.5 M)
80 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v)
200 μl of ethylene glycol BCC (100 v/v)
148 μl of water (100 v/v)
C12
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
571 μl of ammonium sulfate BCC (3.5 M)
70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
328 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 2 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
pH 4.2
571 μl of ammonium sulfate BCC (3.5 M)
112 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
88 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
228 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
571 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
328 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 2 M -
  • DL-malate-imidazole BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
34 μl of DL-malate-imidazole BCC pH 2 (1 M)
66 μl of DL-malate-imidazole BCC pH 9 (1 M)
328 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
100 μl of bis-tris chloride BCC pH 5.5 (1 M)
328 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
40 μl of sodium chloride BCC (5 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
288 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
328 μl of water (100 v/v)
D7
  • ammonium sulfate BCC 2 M -
  • potassium sodium tartrate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
100 μl of potassium sodium tartrate BCC (2 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
228 μl of water (100 v/v)
D8
  • ADA BCC 0.1 M pH 6.5
  • ammonium sulfate BCC 2 M -
  • ethanol BCC 10 v/v -
pH 6.5
571 μl of ammonium sulfate BCC (3.5 M)
109 μl of ADA BCC pH 5.5 (0.5 M)
100 μl of ethanol BCC (100 v/v)
90 μl of ADA BCC pH 7.5 (0.5 M)
128 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
571 μl of ammonium sulfate BCC (3.5 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
40 μl of sodium chloride BCC (5 M)
288 μl of water (100 v/v)
D10
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
571 μl of ammonium sulfate BCC (3.5 M)
100 μl of bis-tris chloride BCC pH 6.5 (1 M)
328 μl of water (100 v/v)
D11
  • ADA BCC 0.1 M pH 6.5
  • ammonium sulfate BCC 2 M -
  • magnesium sulfate BCC 0.1 M -
pH 6.5
571 μl of ammonium sulfate BCC (3.5 M)
40 μl of magnesium sulfate BCC (2.5 M)
109 μl of ADA BCC pH 5.5 (0.5 M)
90 μl of ADA BCC pH 7.5 (0.5 M)
188 μl of water (100 v/v)
D12
  • ammonium sulfate BCC 2 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
571 μl of ammonium sulfate BCC (3.5 M)
80 μl of lithium sulfate BCC (2.5 M)
100 μl of tris chloride BCC pH 7 (1 M)
248 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
328 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 2 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571 μl of ammonium sulfate BCC (3.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
25 μl of polyethylene glycol 400 BCC (80 v/v)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
303 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571 μl of ammonium sulfate BCC (3.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
328 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
571 μl of ammonium sulfate BCC (3.5 M)
100 μl of sodium bicine BCC pH 9.4 (1 M)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
278 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
  • CHES BCC 0.1 M pH 9.5
pH 9.5
571 μl of ammonium sulfate BCC (3.5 M)
50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
253 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 2 M -
  • CAPS BCC 0.1 M pH 10
  • lithium sulfate BCC 0.2 M -
pH 10
571 μl of ammonium sulfate BCC (3.5 M)
114 μl of CAPS BCC pH 11 (0.5 M)
80 μl of lithium sulfate BCC (2.5 M)
86 μl of CAPS BCC pH 9.4 (0.5 M)
148 μl of water (100 v/v)
E7
  • ammonium sulfate BCC 2.45 M -
  • dimethyl sulfoxide BCC 10 v/v -
NA
700 μl of ammonium sulfate BCC (3.5 M)
100 μl of dimethyl sulfoxide BCC (100 v/v)
200 μl of water (100 v/v)
E8
  • ammonium sulfate BCC 2.45 M -
  • glycerol BCC 10 v/v -
NA
700 μl of ammonium sulfate BCC (3.5 M)
125 μl of glycerol BCC (80 v/v)
175 μl of water (100 v/v)
E9
  • ammonium sulfate BCC 2.5 M -
  • ethanol BCC 6 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
714 μl of ammonium sulfate BCC (3.5 M)
60 μl of ethanol BCC (100 v/v)
36 μl of sodium MES BCC pH 7.2 (1 M)
13 μl of sodium MES BCC pH 5.2 (1 M)
175 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 2.7 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
771 μl of ammonium sulfate BCC (3.5 M)
36 μl of sodium MOPS BCC pH 8.2 (0.5 M)
63 μl of sodium MOPS BCC pH 6.2 (0.5 M)
128 μl of water (100 v/v)
E11
  • ammonium sulfate BCC 2.7 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
771 μl of ammonium sulfate BCC (3.5 M)
39 μl of tris chloride BCC pH 9 (1 M)
10 μl of tris chloride BCC pH 7 (1 M)
178 μl of water (100 v/v)
E12
  • ammonium sulfate BCC 2.7 M -
  • CHES BCC 0.05 M pH 9.5
pH 9.5
771 μl of ammonium sulfate BCC (3.5 M)
39 μl of CHES BCC pH 10 (0.8 M)
22 μl of CHES BCC pH 8.3 (0.8 M)
166 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1 M -
  • cesium chloride BCC 2 M -
NA
285 μl of ammonium sulfate BCC (3.5 M)
400 μl of cesium chloride BCC (5 M)
314 μl of water (100 v/v)
F2
  • cadmium sulfate BCC 0.05 M -
  • sodium acetate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
333 μl of sodium acetate BCC (3 M)
100 μl of cadmium sulfate BCC (0.5 M)
466 μl of water (100 v/v)
F3
  • cobalt(II) sulfate BCC 1.5 M -
NA
750 μl of cobalt(II) sulfate BCC (2 M)
250 μl of water (100 v/v)
F4
  • lithium sulfate BCC 0.2 M -
  • sodium chloride BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80 μl of lithium sulfate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
400 μl of sodium chloride BCC (5 M)
420 μl of water (100 v/v)
F5
  • lithium sulfate BCC 0.5 M -
  • sodium cacodylate BCC 0.1 M pH 6
  • trehalose BCC 0.12 M -
pH 6
69 μl of sodium cacodylate BCC pH 5.3 (1 M)
200 μl of lithium sulfate BCC (2.5 M)
30 μl of sodium cacodylate BCC pH 7.3 (1 M)
120 μl of trehalose BCC (1 M)
580 μl of water (100 v/v)
F6
  • lithium sulfate BCC 0.8 M -
  • sodium acetate-acetic acid BCC 0.05 M pH 4.5
pH 4.5
320 μl of lithium sulfate BCC (2.5 M)
15 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
630 μl of water (100 v/v)
F7
  • lithium sulfate BCC 1 M -
  • polyethylene glycol 8000 BCC 2 w/v -
NA
100 μl of polyethylene glycol 8000 BCC (20 w/v)
400 μl of lithium sulfate BCC (2.5 M)
500 μl of water (100 v/v)
F8
  • lithium sulfate BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
400 μl of lithium sulfate BCC (2.5 M)
31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500 μl of water (100 v/v)
F9
  • ammonium sulfate BCC 0.5 M -
  • lithium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
142 μl of ammonium sulfate BCC (3.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
400 μl of lithium sulfate BCC (2.5 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
357 μl of water (100 v/v)
F10
  • lithium sulfate BCC 1 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400 μl of lithium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
25 μl of polyethylene glycol 400 BCC (80 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
475 μl of water (100 v/v)
F11
  • lithium sulfate BCC 1 M -
  • nickel(II) chloride BCC 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400 μl of lithium sulfate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
10 μl of nickel(II) chloride BCC (1 M)
490 μl of water (100 v/v)
F12
  • lithium sulfate BCC 1.3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
520 μl of lithium sulfate BCC (2.5 M)
45 μl of tris chloride BCC pH 9 (1 M)
54 μl of tris chloride BCC pH 7 (1 M)
380 μl of water (100 v/v)
G1
  • lithium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600 μl of lithium sulfate BCC (2.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
300 μl of water (100 v/v)
G2
  • lithium sulfate BCC 1.8 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
36 μl of sodium MOPS BCC pH 8.2 (0.5 M)
720 μl of lithium sulfate BCC (2.5 M)
63 μl of sodium MOPS BCC pH 6.2 (0.5 M)
180 μl of water (100 v/v)
G3
  • CHES BCC 0.1 M pH 9.5
  • lithium sulfate BCC 2 M -
pH 9.5
800 μl of lithium sulfate BCC (2.5 M)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
75 μl of water (100 v/v)
G4
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 0.75 M -
pH 5.5
300 μl of magnesium sulfate BCC (2.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
600 μl of water (100 v/v)
G5
  • sodium MES BCC 0.1 M pH 6
  • trehalose BCC 0.12 M -
  • magnesium sulfate BCC 1 M -
pH 6
400 μl of magnesium sulfate BCC (2.5 M)
38 μl of sodium MES BCC pH 7.2 (1 M)
120 μl of trehalose BCC (1 M)
61 μl of sodium MES BCC pH 5.2 (1 M)
380 μl of water (100 v/v)
G6
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 1.5 M -
pH 5.5
600 μl of magnesium sulfate BCC (2.5 M)
20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
300 μl of water (100 v/v)
G7
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
640 μl of magnesium sulfate BCC (2.5 M)
72 μl of sodium MES BCC pH 7.2 (1 M)
27 μl of sodium MES BCC pH 5.2 (1 M)
260 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trehalose BCC 0.12 M -
  • magnesium sulfate BCC 1.6 M -
pH 7.5
640 μl of magnesium sulfate BCC (2.5 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
120 μl of trehalose BCC (1 M)
140 μl of water (100 v/v)
G9
  • glycerol BCC 15 v/v -
  • magnesium sulfate BCC 2 M -
NA
800 μl of magnesium sulfate BCC (2.5 M)
187 μl of glycerol BCC (80 v/v)
12 μl of water (100 v/v)
G10
  • tris chloride BCC 0.1 M pH 8.5
  • magnesium sulfate BCC 2 M -
pH 8.5
800 μl of magnesium sulfate BCC (2.5 M)
78 μl of tris chloride BCC pH 9 (1 M)
21 μl of tris chloride BCC pH 7 (1 M)
100 μl of water (100 v/v)
G11
  • CHES BCC 0.1 M pH 9.5
  • magnesium sulfate BCC 2 M -
pH 9.5
800 μl of magnesium sulfate BCC (2.5 M)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
75 μl of water (100 v/v)
G12
  • triammonium citrate BCC 1.8 M -
NA
720 μl of triammonium citrate BCC (2.5 M)
280 μl of water (100 v/v)
H1
  • trisodium citrate BCC 0.75 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10 μl of sodium tetraborate BCC pH 8.5 (1 M)
468 μl of trisodium citrate BCC (1.6 M)
521 μl of water (100 v/v)
H2
  • sodium cacodylate BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
34 μl of sodium cacodylate BCC pH 5.3 (1 M)
65 μl of sodium cacodylate BCC pH 7.3 (1 M)
625 μl of trisodium citrate BCC (1.6 M)
275 μl of water (100 v/v)
H3
  • polyethylene glycol 400 BCC 5 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
72 μl of sodium MES BCC pH 7.2 (1 M)
625 μl of trisodium citrate BCC (1.6 M)
62 μl of polyethylene glycol 400 BCC (80 v/v)
27 μl of sodium MES BCC pH 5.2 (1 M)
212 μl of water (100 v/v)
H4
  • imidazole BCC 0.1 M pH 8
  • trisodium citrate BCC 1 M -
pH 8
100 μl of imidazole BCC pH 8 (1 M)
625 μl of trisodium citrate BCC (1.6 M)
275 μl of water (100 v/v)
H5
  • trisodium citrate BCC 1 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10 μl of sodium tetraborate BCC pH 8.5 (1 M)
625 μl of trisodium citrate BCC (1.6 M)
365 μl of water (100 v/v)
H6
  • CHES BCC 0.1 M pH 9.5
  • trisodium citrate BCC 1 M -
pH 9.5
625 μl of trisodium citrate BCC (1.6 M)
79 μl of CHES BCC pH 10 (0.8 M)
45 μl of CHES BCC pH 8.3 (0.8 M)
250 μl of water (100 v/v)
H7
  • sodium cacodylate BCC 0.06 M pH 6
  • trisodium citrate BCC 1.4 M -
pH 6
41 μl of sodium cacodylate BCC pH 5.3 (1 M)
18 μl of sodium cacodylate BCC pH 7.3 (1 M)
875 μl of trisodium citrate BCC (1.6 M)
65 μl of water (100 v/v)
H8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 1.4 M -
pH 7.5
875 μl of trisodium citrate BCC (1.6 M)
50 μl of sodium HEPES BCC pH 6.5 (1 M)
50 μl of sodium HEPES BCC pH 8.5 (1 M)
25 μl of water (100 v/v)
H9
  • tris chloride BCC 0.06 M pH 8.5
  • trisodium citrate BCC 1.4 M -
pH 8.5
47 μl of tris chloride BCC pH 9 (1 M)
12 μl of tris chloride BCC pH 7 (1 M)
875 μl of trisodium citrate BCC (1.6 M)
65 μl of water (100 v/v)
H10
  • trisodium citrate BCC 1.5 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10 μl of sodium tetraborate BCC pH 8.5 (1 M)
937 μl of trisodium citrate BCC (1.6 M)
52 μl of water (100 v/v)
H11
  • trisodium citrate BCC 1.2 M -
NA
750 μl of trisodium citrate BCC (1.6 M)
250 μl of water (100 v/v)
H12
  • trisodium citrate BCC 1.6 M -
NA
1000 μl of trisodium citrate BCC (1.6 M)