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Supplemental Information

Screen Description

c3_5

Created 2025-03-07.
Salty: ammonium sulfate, other sulfates, citrates

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c3_5

  • Z0002712 Lot 1 (1500.0 µl)
  • Z0002713 Lot 2 (1500.0 µl)
  • Z0002644 Lot 3 (0.0 µl)

Screen Structure

c3_5

Well Count 96
Number of distinct ingredients 46
Internal diversity (score mean dist) 0.877

Screen Composition

2025-08-15T23:26:01.785870 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type c3_5
Buffer 91
Cryoprotectant 8
Detergent 2
Organic (non-volatile) 14
Organic (volatile) 7
Polymer 7
Salt 138

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_5 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • ammonium sulfate BCC 0.75 M -
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 0.2 M -
pH 6.5
214.3 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of magnesium sulfate BCC (2.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
605.7 μl of water (100 v/v)
A2
  • ammonium sulfate BCC 1 M -
  • copper(II) sulfate BCC 0.02 M -
NA
285.7 μl of ammonium sulfate BCC (3.5 M)
20.0 μl of copper(II) sulfate BCC (1 M)
694.3 μl of water (100 v/v)
A3
  • ammonium sulfate BCC 1 M -
  • potassium chloride BCC 1 M -
NA
285.7 μl of ammonium sulfate BCC (3.5 M)
333.3 μl of potassium chloride BCC (3 M)
381.0 μl of water (100 v/v)
A4
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 0.2 M -
  • trehalose BCC 0.12 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
285.7 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of lithium sulfate BCC (2.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
120.0 μl of trehalose BCC (1 M)
414.3 μl of water (100 v/v)
A5
  • ammonium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.15 M pH 5.5
pH 5.5
285.7 μl of ammonium sulfate BCC (3.5 M)
120.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
30.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
564.3 μl of water (100 v/v)
A6
  • ammonium sulfate BCC 1 M -
  • polyethylene glycol 3350 BCC 1 w/v -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
285.7 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
50.0 μl of polyethylene glycol 3350 BCC (20 w/v)
564.3 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
285.7 μl of ammonium sulfate BCC (3.5 M)
40.0 μl of sodium chloride BCC (5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
574.3 μl of water (100 v/v)
A8
  • ammonium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • polyethylene glycol 8000 BCC 0.5 w/v -
pH 7
285.7 μl of ammonium sulfate BCC (3.5 M)
25.0 μl of polyethylene glycol 8000 BCC (20 w/v)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
589.3 μl of water (100 v/v)
A9
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7
  • trehalose BCC 0.075 M -
pH 7
285.7 μl of ammonium sulfate BCC (3.5 M)
400.0 μl of lithium sulfate BCC (2.5 M)
81.7 μl of sodium HEPES BCC pH 6.5 (1 M)
18.3 μl of sodium HEPES BCC pH 8.5 (1 M)
75.0 μl of trehalose BCC (1 M)
139.3 μl of water (100 v/v)
A10
  • ammonium sulfate BCC 1 M -
  • lithium sulfate BCC 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
285.7 μl of ammonium sulfate BCC (3.5 M)
400.0 μl of lithium sulfate BCC (2.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
214.3 μl of water (100 v/v)
A11
  • ammonium sulfate BCC 1.26 M -
  • sodium chloride BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
360.0 μl of ammonium sulfate BCC (3.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
40.0 μl of sodium chloride BCC (5 M)
500.0 μl of water (100 v/v)
A12
  • ammonium sulfate BCC 1.26 M -
  • sodium MES BCC 0.1 M pH 6
pH 6
360.0 μl of ammonium sulfate BCC (3.5 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
540.0 μl of water (100 v/v)
B1
  • ammonium sulfate BCC 1.26 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
360.0 μl of ammonium sulfate BCC (3.5 M)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
540.0 μl of water (100 v/v)
B2
  • ammonium sulfate BCC 1.26 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
360.0 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
540.0 μl of water (100 v/v)
B3
  • ammonium sulfate BCC 1.26 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
360.0 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of lithium sulfate BCC (2.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
460.0 μl of water (100 v/v)
B4
  • ammonium sulfate BCC 1.26 M -
  • CHES BCC 0.1 M pH 9.5
  • sodium chloride BCC 0.2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
360.0 μl of ammonium sulfate BCC (3.5 M)
40.0 μl of sodium chloride BCC (5 M)
475.0 μl of water (100 v/v)
B5
  • ammonium sulfate BCC 1.5 M -
  • tergitol NP-40 0.01 w/v -
NA
428.6 μl of ammonium sulfate BCC (3.5 M)
10.0 μl of tergitol NP-40 (1 w/v)
561.4 μl of water (100 v/v)
B6
  • ammonium sulfate BCC 1.5 M -
  • 1-octanol BCC 2.5 v/v -
NA
25.0 μl of 1-octanol BCC (100 v/v)
428.6 μl of ammonium sulfate BCC (3.5 M)
546.4 μl of water (100 v/v)
B7
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 10 v/v -
  • magnesium chloride BCC 0.05 M -
  • potassium chloride BCC 0.05 M -
NA
428.6 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
50.0 μl of magnesium chloride BCC (1 M)
16.7 μl of potassium chloride BCC (3 M)
379.8 μl of water (100 v/v)
B8
  • ammonium sulfate BCC 1.5 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • trehalose BCC 0.12 M -
pH 5.5
428.6 μl of ammonium sulfate BCC (3.5 M)
40.0 μl of sodium chloride BCC (5 M)
120.0 μl of trehalose BCC (1 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
311.4 μl of water (100 v/v)
B9
  • ammonium sulfate BCC 1.5 M -
  • DL-malate-imidazole BCC 0.1 M pH 5.5
pH 5.5
34.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
66.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
428.6 μl of ammonium sulfate BCC (3.5 M)
471.4 μl of water (100 v/v)
B10
  • ammonium sulfate BCC 1.5 M -
  • trisodium citrate-citric acid BCC 0.15 M pH 5.5
pH 5.5
428.6 μl of ammonium sulfate BCC (3.5 M)
120.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
30.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
421.4 μl of water (100 v/v)
B11
  • ammonium sulfate BCC 1.5 M -
  • sodium cacodylate BCC 0.05 M pH 6
pH 6
428.6 μl of ammonium sulfate BCC (3.5 M)
34.6 μl of sodium cacodylate BCC pH 5.3 (1 M)
15.4 μl of sodium cacodylate BCC pH 7.3 (1 M)
521.4 μl of water (100 v/v)
B12
  • ammonium sulfate BCC 1.5 M -
  • sodium chloride BCC 0.1 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
428.6 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
20.0 μl of sodium chloride BCC (5 M)
451.4 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 1.5 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
  • trehalose BCC 0.12 M -
pH 7.5
428.6 μl of ammonium sulfate BCC (3.5 M)
25.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
120.0 μl of trehalose BCC (1 M)
326.4 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 1.5 M -
  • glycerol BCC 12 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
428.6 μl of ammonium sulfate BCC (3.5 M)
150.0 μl of glycerol BCC (80 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
321.4 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 1.6 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
457.1 μl of ammonium sulfate BCC (3.5 M)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
442.9 μl of water (100 v/v)
C4
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
457.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of dioxane BCC (100 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
342.9 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 1.6 M -
  • dioxane BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457.1 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of dioxane BCC (100 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 1.6 M -
  • sodium chloride BCC 0.1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
457.1 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
20.0 μl of sodium chloride BCC (5 M)
422.9 μl of water (100 v/v)
C7
  • ammonium sulfate BCC 1.8 M -
  • cobalt chloride BCC 0.01 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
514.3 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of cobalt chloride BCC (0.1 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
285.7 μl of water (100 v/v)
C8
  • 2-propanol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
NA
62.5 μl of 2-propanol BCC (80 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
366.1 μl of water (100 v/v)
C9
  • ammonium sulfate BCC 2 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
428.6 μl of water (100 v/v)
C10
  • ammonium sulfate BCC 2 M -
  • sodium chloride BCC 1 M -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
200.0 μl of sodium chloride BCC (5 M)
228.6 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 2 M -
  • ethylene glycol BCC 20 v/v -
  • n-octyl-b-D-Glucopyranoside BCC 0.8 w/v -
NA
571.4 μl of ammonium sulfate BCC (3.5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
80.0 μl of n-octyl-b-D-Glucopyranoside BCC (10 w/v)
148.6 μl of water (100 v/v)
C12
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
571.4 μl of ammonium sulfate BCC (3.5 M)
30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
328.6 μl of water (100 v/v)
D1
  • ammonium sulfate BCC 2 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
pH 4.2
571.4 μl of ammonium sulfate BCC (3.5 M)
88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
112.0 μl of phosphate-citrate BCC pH 9.3 (0.5 M)
228.6 μl of water (100 v/v)
D2
  • ammonium sulfate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
571.4 μl of ammonium sulfate BCC (3.5 M)
65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
328.6 μl of water (100 v/v)
D3
  • ammonium sulfate BCC 2 M -
  • DL-malate-imidazole BCC 0.1 M pH 5.5
pH 5.5
34.0 μl of DL-malate-imidazole BCC pH 2 (1 M)
66.0 μl of DL-malate-imidazole BCC pH 9 (1 M)
571.4 μl of ammonium sulfate BCC (3.5 M)
328.6 μl of water (100 v/v)
D4
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
328.6 μl of water (100 v/v)
D5
  • ammonium sulfate BCC 2 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
40.0 μl of sodium chloride BCC (5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
288.6 μl of water (100 v/v)
D6
  • ammonium sulfate BCC 2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
328.6 μl of water (100 v/v)
D7
  • ammonium sulfate BCC 2 M -
  • potassium sodium tartrate BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of potassium sodium tartrate BCC (2 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
228.6 μl of water (100 v/v)
D8
  • ADA BCC 0.1 M pH 6.5
  • ammonium sulfate BCC 2 M -
  • ethanol BCC 10 v/v -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of ethanol BCC (100 v/v)
128.6 μl of water (100 v/v)
D9
  • ammonium sulfate BCC 2 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
40.0 μl of sodium chloride BCC (5 M)
288.6 μl of water (100 v/v)
D10
  • ammonium sulfate BCC 2 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
328.6 μl of water (100 v/v)
D11
  • ADA BCC 0.1 M pH 6.5
  • ammonium sulfate BCC 2 M -
  • magnesium sulfate BCC 0.1 M -
pH 6.5
109.4 μl of ADA BCC pH 5.5 (0.5 M)
90.6 μl of ADA BCC pH 7.5 (0.5 M)
571.4 μl of ammonium sulfate BCC (3.5 M)
40.0 μl of magnesium sulfate BCC (2.5 M)
188.6 μl of water (100 v/v)
D12
  • ammonium sulfate BCC 2 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of lithium sulfate BCC (2.5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
248.6 μl of water (100 v/v)
E1
  • ammonium sulfate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
E2
  • ammonium sulfate BCC 2 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
571.4 μl of ammonium sulfate BCC (3.5 M)
25.0 μl of polyethylene glycol 400 BCC (80 v/v)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
303.6 μl of water (100 v/v)
E3
  • ammonium sulfate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of ammonium sulfate BCC (3.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
328.6 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
571.4 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of sodium bicine BCC pH 9.4 (1 M)
278.6 μl of water (100 v/v)
E5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • ammonium sulfate BCC 2 M -
  • CHES BCC 0.1 M pH 9.5
pH 9.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
571.4 μl of ammonium sulfate BCC (3.5 M)
253.6 μl of water (100 v/v)
E6
  • ammonium sulfate BCC 2 M -
  • CAPS BCC 0.1 M pH 10
  • lithium sulfate BCC 0.2 M -
pH 10
86.0 μl of CAPS BCC pH 9.4 (0.5 M)
114.0 μl of CAPS BCC pH 11 (0.5 M)
571.4 μl of ammonium sulfate BCC (3.5 M)
80.0 μl of lithium sulfate BCC (2.5 M)
148.6 μl of water (100 v/v)
E7
  • ammonium sulfate BCC 2.45 M -
  • dimethyl sulfoxide BCC 10 v/v -
NA
700.0 μl of ammonium sulfate BCC (3.5 M)
100.0 μl of dimethyl sulfoxide BCC (100 v/v)
200.0 μl of water (100 v/v)
E8
  • ammonium sulfate BCC 2.45 M -
  • glycerol BCC 10 v/v -
NA
700.0 μl of ammonium sulfate BCC (3.5 M)
125.0 μl of glycerol BCC (80 v/v)
175.0 μl of water (100 v/v)
E9
  • ammonium sulfate BCC 2.5 M -
  • ethanol BCC 6 v/v -
  • sodium MES BCC 0.05 M pH 6.5
pH 6.5
714.3 μl of ammonium sulfate BCC (3.5 M)
60.0 μl of ethanol BCC (100 v/v)
13.9 μl of sodium MES BCC pH 5.2 (1 M)
36.1 μl of sodium MES BCC pH 7.2 (1 M)
175.7 μl of water (100 v/v)
E10
  • ammonium sulfate BCC 2.7 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
771.4 μl of ammonium sulfate BCC (3.5 M)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
128.6 μl of water (100 v/v)
E11
  • ammonium sulfate BCC 2.7 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
771.4 μl of ammonium sulfate BCC (3.5 M)
10.6 μl of tris chloride BCC pH 7 (1 M)
39.4 μl of tris chloride BCC pH 9 (1 M)
178.6 μl of water (100 v/v)
E12
  • ammonium sulfate BCC 2.7 M -
  • CHES BCC 0.05 M pH 9.5
pH 9.5
39.9 μl of CHES BCC pH 10 (0.8 M)
22.6 μl of CHES BCC pH 8.3 (0.8 M)
771.4 μl of ammonium sulfate BCC (3.5 M)
166.1 μl of water (100 v/v)
F1
  • ammonium sulfate BCC 1 M -
  • cesium chloride BCC 2 M -
NA
285.7 μl of ammonium sulfate BCC (3.5 M)
400.0 μl of cesium chloride BCC (5 M)
314.3 μl of water (100 v/v)
F2
  • cadmium sulfate BCC 0.05 M -
  • sodium acetate BCC 1 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
100.0 μl of cadmium sulfate BCC (0.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
333.3 μl of sodium acetate BCC (3 M)
466.7 μl of water (100 v/v)
F3
  • cobalt(II) sulfate BCC 1.5 M -
NA
750.0 μl of cobalt(II) sulfate BCC (2 M)
250.0 μl of water (100 v/v)
F4
  • lithium sulfate BCC 0.2 M -
  • sodium chloride BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of lithium sulfate BCC (2.5 M)
400.0 μl of sodium chloride BCC (5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
420.0 μl of water (100 v/v)
F5
  • lithium sulfate BCC 0.5 M -
  • sodium cacodylate BCC 0.1 M pH 6
  • trehalose BCC 0.12 M -
pH 6
200.0 μl of lithium sulfate BCC (2.5 M)
69.1 μl of sodium cacodylate BCC pH 5.3 (1 M)
30.9 μl of sodium cacodylate BCC pH 7.3 (1 M)
120.0 μl of trehalose BCC (1 M)
580.0 μl of water (100 v/v)
F6
  • lithium sulfate BCC 0.8 M -
  • sodium acetate-acetic acid BCC 0.05 M pH 4.5
pH 4.5
320.0 μl of lithium sulfate BCC (2.5 M)
15.6 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.4 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
630.0 μl of water (100 v/v)
F7
  • lithium sulfate BCC 1 M -
  • polyethylene glycol 8000 BCC 2 w/v -
NA
400.0 μl of lithium sulfate BCC (2.5 M)
100.0 μl of polyethylene glycol 8000 BCC (20 w/v)
500.0 μl of water (100 v/v)
F8
  • lithium sulfate BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
400.0 μl of lithium sulfate BCC (2.5 M)
31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of water (100 v/v)
F9
  • ammonium sulfate BCC 0.5 M -
  • lithium sulfate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
142.9 μl of ammonium sulfate BCC (3.5 M)
400.0 μl of lithium sulfate BCC (2.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
357.1 μl of water (100 v/v)
F10
  • lithium sulfate BCC 1 M -
  • polyethylene glycol 400 BCC 2 v/v -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
400.0 μl of lithium sulfate BCC (2.5 M)
25.0 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
475.0 μl of water (100 v/v)
F11
  • lithium sulfate BCC 1 M -
  • nickel(II) chloride BCC 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of lithium sulfate BCC (2.5 M)
10.0 μl of nickel(II) chloride BCC (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)
F12
  • lithium sulfate BCC 1.3 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
520.0 μl of lithium sulfate BCC (2.5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
380.0 μl of water (100 v/v)
G1
  • lithium sulfate BCC 1.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of lithium sulfate BCC (2.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G2
  • lithium sulfate BCC 1.8 M -
  • sodium MOPS BCC 0.05 M pH 7
pH 7
720.0 μl of lithium sulfate BCC (2.5 M)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
180.0 μl of water (100 v/v)
G3
  • CHES BCC 0.1 M pH 9.5
  • lithium sulfate BCC 2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
800.0 μl of lithium sulfate BCC (2.5 M)
75.0 μl of water (100 v/v)
G4
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 0.75 M -
pH 5.5
300.0 μl of magnesium sulfate BCC (2.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
600.0 μl of water (100 v/v)
G5
  • sodium MES BCC 0.1 M pH 6
  • trehalose BCC 0.12 M -
  • magnesium sulfate BCC 1 M -
pH 6
400.0 μl of magnesium sulfate BCC (2.5 M)
61.6 μl of sodium MES BCC pH 5.2 (1 M)
38.4 μl of sodium MES BCC pH 7.2 (1 M)
120.0 μl of trehalose BCC (1 M)
380.0 μl of water (100 v/v)
G6
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
  • magnesium sulfate BCC 1.5 M -
pH 5.5
600.0 μl of magnesium sulfate BCC (2.5 M)
80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
300.0 μl of water (100 v/v)
G7
  • sodium MES BCC 0.1 M pH 6.5
  • magnesium sulfate BCC 1.6 M -
pH 6.5
640.0 μl of magnesium sulfate BCC (2.5 M)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
260.0 μl of water (100 v/v)
G8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trehalose BCC 0.12 M -
  • magnesium sulfate BCC 1.6 M -
pH 7.5
640.0 μl of magnesium sulfate BCC (2.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
120.0 μl of trehalose BCC (1 M)
140.0 μl of water (100 v/v)
G9
  • glycerol BCC 15 v/v -
  • magnesium sulfate BCC 2 M -
NA
187.5 μl of glycerol BCC (80 v/v)
800.0 μl of magnesium sulfate BCC (2.5 M)
12.5 μl of water (100 v/v)
G10
  • tris chloride BCC 0.1 M pH 8.5
  • magnesium sulfate BCC 2 M -
pH 8.5
800.0 μl of magnesium sulfate BCC (2.5 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
G11
  • CHES BCC 0.1 M pH 9.5
  • magnesium sulfate BCC 2 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
800.0 μl of magnesium sulfate BCC (2.5 M)
75.0 μl of water (100 v/v)
G12
  • triammonium citrate BCC 1.8 M -
NA
720.0 μl of triammonium citrate BCC (2.5 M)
280.0 μl of water (100 v/v)
H1
  • trisodium citrate BCC 0.75 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10.0 μl of sodium tetraborate BCC pH 8.5 (1 M)
468.8 μl of trisodium citrate BCC (1.6 M)
521.2 μl of water (100 v/v)
H2
  • sodium cacodylate BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
34.9 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.1 μl of sodium cacodylate BCC pH 7.3 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
275.0 μl of water (100 v/v)
H3
  • polyethylene glycol 400 BCC 5 v/v -
  • sodium MES BCC 0.1 M pH 6.5
  • trisodium citrate BCC 1 M -
pH 6.5
62.5 μl of polyethylene glycol 400 BCC (80 v/v)
27.8 μl of sodium MES BCC pH 5.2 (1 M)
72.2 μl of sodium MES BCC pH 7.2 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
212.5 μl of water (100 v/v)
H4
  • imidazole BCC 0.1 M pH 8
  • trisodium citrate BCC 1 M -
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
275.0 μl of water (100 v/v)
H5
  • trisodium citrate BCC 1 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10.0 μl of sodium tetraborate BCC pH 8.5 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
365.0 μl of water (100 v/v)
H6
  • CHES BCC 0.1 M pH 9.5
  • trisodium citrate BCC 1 M -
pH 9.5
79.8 μl of CHES BCC pH 10 (0.8 M)
45.2 μl of CHES BCC pH 8.3 (0.8 M)
625.0 μl of trisodium citrate BCC (1.6 M)
250.0 μl of water (100 v/v)
H7
  • sodium cacodylate BCC 0.06 M pH 6
  • trisodium citrate BCC 1.4 M -
pH 6
41.5 μl of sodium cacodylate BCC pH 5.3 (1 M)
18.5 μl of sodium cacodylate BCC pH 7.3 (1 M)
875.0 μl of trisodium citrate BCC (1.6 M)
65.0 μl of water (100 v/v)
H8
  • sodium HEPES BCC 0.1 M pH 7.5
  • trisodium citrate BCC 1.4 M -
pH 7.5
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
875.0 μl of trisodium citrate BCC (1.6 M)
25.0 μl of water (100 v/v)
H9
  • tris chloride BCC 0.06 M pH 8.5
  • trisodium citrate BCC 1.4 M -
pH 8.5
12.7 μl of tris chloride BCC pH 7 (1 M)
47.3 μl of tris chloride BCC pH 9 (1 M)
875.0 μl of trisodium citrate BCC (1.6 M)
65.0 μl of water (100 v/v)
H10
  • trisodium citrate BCC 1.5 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
10.0 μl of sodium tetraborate BCC pH 8.5 (1 M)
937.5 μl of trisodium citrate BCC (1.6 M)
52.5 μl of water (100 v/v)
H11
  • trisodium citrate BCC 1.2 M -
NA
750.0 μl of trisodium citrate BCC (1.6 M)
250.0 μl of water (100 v/v)
H12
  • trisodium citrate BCC 1.6 M -
NA
1000.0 μl of trisodium citrate BCC (1.6 M)