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Supplemental Information

Screen Description

Memb-PASS / Memb-PASS Solubility screen
Created 2012-03-08.

Rigaku Recipe XML

μl
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Screen Structure

Well Count 96
Number of distinct ingredients 12
Internal diversity (score mean dist) 0.9

Screen Composition

More information about screen composition chart.
Chemical classes bar chart
ingredient type Memb-PASS Solubility screen
Buffer 4
Organic (non-volatile) 2
Polymer 24
Salt 10

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Memb-PASS Solubility screen Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Polyethylene glycol 400 50 v/v -
NA
500.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
A2
  • Polyethylene glycol monomethyl ether 2,000 35 w/v -
NA
700.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
300.0 μl of water (100 v/v)
A3
  • Polyethylene glycol monomethyl ether 2,000 35 w/v -
  • Magnesium acetate 0.1 M -
NA
100.0 μl of Magnesium acetate (1 M)
700.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of water (100 v/v)
A4 NA
A5 NA
A6 NA
A7 NA
A8 NA
A9 NA
A10 NA
A11 NA
A12 NA
B1
  • Polyethylene glycol 400 25 v/v -
NA
250.0 μl of Polyethylene glycol 400 (100 v/v)
750.0 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
NA
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
660.0 μl of water (100 v/v)
B3
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
  • Magnesium acetate 0.1 M -
NA
100.0 μl of Magnesium acetate (1 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
560.0 μl of water (100 v/v)
B4 NA
B5 NA
B6 NA
B7 NA
B8 NA
B9 NA
B10 NA
B11 NA
B12 NA
C1
  • Polyethylene glycol monomethyl ether 550 40 v/v -
NA
400.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
600.0 μl of water (100 v/v)
C2
  • Polyethylene glycol monomethyl ether 2,000 35 w/v -
  • Potassium thiocyanate 0.1 M -
NA
700.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
287.5 μl of water (100 v/v)
C3
  • Polyethylene glycol 1,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 4.5
pH 4.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
C4 NA
C5 NA
C6 NA
C7 NA
C8 NA
C9 NA
C10 NA
C11 NA
C12 NA
D1
  • Polyethylene glycol monomethyl ether 550 20 v/v -
NA
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
800.0 μl of water (100 v/v)
D2
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
NA
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
647.5 μl of water (100 v/v)
D3
  • Polyethylene glycol 1,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
D4 NA
D5 NA
D6 NA
D7 NA
D8 NA
D9 NA
D10 NA
D11 NA
D12 NA
E1
  • Polyethylene glycol monomethyl ether 2,000 35 w/v -
  • Lithium sulfate 0.1 M -
NA
100.0 μl of Lithium sulfate (1 M)
700.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 4,000 25 w/v -
NA
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
E3
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
  • tri-Sodium citrate 0.1 M pH 4.5
pH 4.5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
E4 NA
E5 NA
E6 NA
E7 NA
E8 NA
E9 NA
E10 NA
E11 NA
E12 NA
F1
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
  • Lithium sulfate 0.1 M -
NA
100.0 μl of Lithium sulfate (1 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
560.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 4,000 12 w/v -
NA
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
760.0 μl of water (100 v/v)
F3
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
F4 NA
F5 NA
F6 NA
F7 NA
F8 NA
F9 NA
F10 NA
F11 NA
F12 NA
G1
  • Polyethylene glycol 1,000 36 w/v -
NA
720.0 μl of Polyethylene glycol 1,000 (50 w/v)
280.0 μl of water (100 v/v)
G2
  • Polyethylene glycol monomethyl ether 5,000 22 w/v -
NA
440.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
560.0 μl of water (100 v/v)
G3
  • Polyethylene glycol monomethyl ether 2,000 35 w/v -
  • Ammonium nitrate 0.1 M -
NA
10.0 μl of Ammonium nitrate (10 M)
700.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
290.0 μl of water (100 v/v)
G4 NA
G5 NA
G6 NA
G7 NA
G8 NA
G9 NA
G10 NA
G11 NA
G12 NA
H1
  • Polyethylene glycol 1,000 18 w/v -
NA
360.0 μl of Polyethylene glycol 1,000 (50 w/v)
640.0 μl of water (100 v/v)
H2
  • Polyethylene glycol monomethyl ether 5,000 11 w/v -
NA
220.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
780.0 μl of water (100 v/v)
H3
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
  • Ammonium nitrate 0.1 M -
NA
10.0 μl of Ammonium nitrate (10 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
650.0 μl of water (100 v/v)
H4 NA
H5 NA
H6 NA
H7 NA
H8 NA
H9 NA
H10 NA
H11 NA
H12 NA