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Supplemental Information

Screen Description

Buffer Screen 9g

Buffer Screens / Buffer Screen 9g
Created 2025-04-10.

Export

Buffer Screen 9g

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Buffer Screen 9g

  • Z0002211 Lot 1 (0.0 µl)

Screen Structure

Buffer Screen 9g

Well Count 96
Number of distinct ingredients 15
Internal diversity (score mean dist) 0.452

Screen Composition

More information about screen composition chart.
2025-08-15T16:05:53.688830 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Buffer Screen 9g
Buffer 78
Organic (non-volatile) 84
Salt 90

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Buffer Screen 9g Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1NA
1000.0 μl of water (100 v/v)
A2NA
1000.0 μl of water (100 v/v)
A3NA
1000.0 μl of water (100 v/v)
A4
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.05 M -
NA
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
887.5 μl of water (100 v/v)
A5
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.05 M -
NA
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
887.5 μl of water (100 v/v)
A6
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.05 M -
NA
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
887.5 μl of water (100 v/v)
A7
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.2 M -
NA
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
737.5 μl of water (100 v/v)
A8
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.2 M -
NA
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
737.5 μl of water (100 v/v)
A9
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.2 M -
NA
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
737.5 μl of water (100 v/v)
A10NA
1000.0 μl of water (100 v/v)
A11NA
1000.0 μl of water (100 v/v)
A12NA
1000.0 μl of water (100 v/v)
B1
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.05 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B2
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.05 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B3
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.05 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B4
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.2 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
B5
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.2 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
B6
  • glycerol BCC 5 v/v -
  • sodium acetate-acetic acid BCC 0.05 M pH 5
  • sodium chloride BCC 0.2 M -
pH 5
62.5 μl of glycerol BCC (80 v/v)
32.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
17.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
B7
  • glycerol BCC 5 v/v -
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.05 M -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B8
  • glycerol BCC 5 v/v -
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.05 M -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B9
  • glycerol BCC 5 v/v -
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.05 M -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
B10
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
B11
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
B12
  • piperazine BCC 0.05 M pH 5.5
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 5.5
62.5 μl of glycerol BCC (80 v/v)
25.2 μl of piperazine BCC pH 4.4 (1 M)
24.8 μl of piperazine BCC pH 6.4 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
C1
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
  • glycerol BCC 5 v/v -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
C2
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
  • glycerol BCC 5 v/v -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
C3
  • glycerol BCC 5 v/v -
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
C4
  • glycerol BCC 5 v/v -
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
C5
  • glycerol BCC 5 v/v -
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
C6
  • glycerol BCC 5 v/v -
  • sodium MES BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
30.8 μl of sodium MES BCC pH 5.2 (1 M)
19.2 μl of sodium MES BCC pH 7.2 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
C7
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
837.5 μl of water (100 v/v)
C8
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
837.5 μl of water (100 v/v)
C9
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.05 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
837.5 μl of water (100 v/v)
C10
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
687.5 μl of water (100 v/v)
C11
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
687.5 μl of water (100 v/v)
C12
  • glycerol BCC 5 v/v -
  • trisodium citrate-citric acid BCC 0.05 M pH 6
  • sodium chloride BCC 0.2 M -
pH 6
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
45.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
5.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
687.5 μl of water (100 v/v)
D1
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
D2
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
D3
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
D4
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
D5
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
D6
  • glycerol BCC 5 v/v -
  • bis-tris chloride BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
50.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
D7
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
D8
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
D9
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.05 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
D10
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
D11
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
D12
  • glycerol BCC 5 v/v -
  • ADA BCC 0.05 M pH 6.5
  • sodium chloride BCC 0.2 M -
pH 6.5
54.7 μl of ADA BCC pH 5.5 (0.5 M)
45.3 μl of ADA BCC pH 7.5 (0.5 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
E1
  • glycerol BCC 5 v/v -
  • imidazole BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
E2
  • glycerol BCC 5 v/v -
  • imidazole BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
E3
  • glycerol BCC 5 v/v -
  • imidazole BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
E4
  • glycerol BCC 5 v/v -
  • imidazole BCC 0.05 M pH 7
  • sodium chloride BCC 0.2 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
E5
  • glycerol BCC 5 v/v -
  • sodium chloride BCC 0.2 M -
  • imidazole BCC 0.05 M pH 7
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
E6
  • glycerol BCC 5 v/v -
  • imidazole BCC 0.05 M pH 7
  • sodium chloride BCC 0.2 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
26.2 μl of imidazole BCC pH 6 (1 M)
23.8 μl of imidazole BCC pH 8 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
E7
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
E8
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
E9
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.05 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
50.0 μl of sodium chloride BCC (1 M)
787.5 μl of water (100 v/v)
E10
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.2 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
E11
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.2 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
E12
  • glycerol BCC 5 v/v -
  • sodium MOPS BCC 0.05 M pH 7
  • sodium chloride BCC 0.2 M -
pH 7
62.5 μl of glycerol BCC (80 v/v)
63.8 μl of sodium MOPS BCC pH 6.2 (0.5 M)
36.2 μl of sodium MOPS BCC pH 8.2 (0.5 M)
200.0 μl of sodium chloride BCC (1 M)
637.5 μl of water (100 v/v)
F1
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F2
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F3
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F4
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
F5
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
F6
  • glycerol BCC 5 v/v -
  • sodium HEPES BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
62.5 μl of glycerol BCC (80 v/v)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
F7
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F8
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F9
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.05 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
F10
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
F11
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
F12
  • glycerol BCC 5 v/v -
  • disodium hydrogen-potassium dihydrogen phosphate BCC 0.05 M pH 7.5
  • sodium chloride BCC 0.2 M -
pH 7.5
8.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 4.6 (1 M)
42.0 μl of disodium hydrogen-potassium dihydrogen phosphate BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
G1
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.05 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
837.5 μl of water (100 v/v)
G2
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.05 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
837.5 μl of water (100 v/v)
G3
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.05 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
837.5 μl of water (100 v/v)
G4
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.2 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
687.5 μl of water (100 v/v)
G5
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.2 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
687.5 μl of water (100 v/v)
G6
  • glycerol BCC 5 v/v -
  • tris chloride BCC 0.05 M pH 8
  • sodium chloride BCC 0.2 M -
pH 8
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
687.5 μl of water (100 v/v)
G7
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.05 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
G8
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.05 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
G9
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.05 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
837.5 μl of water (100 v/v)
G10
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.2 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
G11
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.2 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
G12
  • glycerol BCC 5 v/v -
  • gly-gly BCC 0.05 M pH 8.5
  • sodium chloride BCC 0.2 M -
pH 8.5
17.0 μl of gly-gly BCC pH 7.3 (1 M)
33.0 μl of gly-gly BCC pH 9.3 (1 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
687.5 μl of water (100 v/v)
H1NA
1000.0 μl of water (100 v/v)
H2NA
1000.0 μl of water (100 v/v)
H3NA
1000.0 μl of water (100 v/v)
H4
  • glycerol BCC 5 v/v -
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.05 M -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
825.0 μl of water (100 v/v)
H5
  • glycerol BCC 5 v/v -
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.05 M -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
825.0 μl of water (100 v/v)
H6
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.05 M -
  • glycerol BCC 5 v/v -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
50.0 μl of sodium chloride BCC (1 M)
825.0 μl of water (100 v/v)
H7
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
675.0 μl of water (100 v/v)
H8
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
675.0 μl of water (100 v/v)
H9
  • CHES BCC 0.05 M pH 9
  • sodium chloride BCC 0.2 M -
  • glycerol BCC 5 v/v -
pH 9
18.6 μl of CHES BCC pH 10 (0.8 M)
43.9 μl of CHES BCC pH 8.3 (0.8 M)
62.5 μl of glycerol BCC (80 v/v)
200.0 μl of sodium chloride BCC (1 M)
675.0 μl of water (100 v/v)
H10NA
1000.0 μl of water (100 v/v)
H11NA
1000.0 μl of water (100 v/v)
H12NA
1000.0 μl of water (100 v/v)