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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
c3_6
BCC Core Screens / c3_6
Created 2025-03-06.
Salty: various phosphates, chlorides, acetates, formates, tartrates
Export
c3_6
Barcodes
c3_6
- Z0002645
- Z0002646
- Z0002710
- Z0002711
Screen Structure
c3_6
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.946 |
Screen Composition
ingredient type | c3_6 |
---|---|
Buffer | 84 |
Cryoprotectant | 6 |
Detergent | 2 |
Organic (non-volatile) | 8 |
Organic (volatile) | 3 |
Polymer | 8 |
Salt | 142 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 160 μl of ammonium dihydrogen phosphate BCC (2.5 M) 840 μl of water (100 v/v) |
A2 |
| pH 5.5 | 400 μl of ammonium dihydrogen phosphate BCC (2.5 M) 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 500 μl of water (100 v/v) |
A3 |
| NA | 600 μl of ammonium dihydrogen phosphate BCC (2.5 M) 400 μl of water (100 v/v) |
A4 |
| pH 8.5 | 800 μl of ammonium dihydrogen phosphate BCC (2.5 M) 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 100 μl of water (100 v/v) |
A5 |
| pH 9 | 37 μl of CHES BCC pH 10 (0.8 M) 87 μl of CHES BCC pH 8.3 (0.8 M) 120 μl of magnesium chloride BCC (2.5 M) 375 μl of ammonium formate BCC (4 M) 380 μl of water (100 v/v) |
A6 |
| pH 7.5 | 500 μl of ammonium formate BCC (4 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 400 μl of water (100 v/v) |
A7 |
| pH 6.5 | 80 μl of ammonium dihydrogen phosphate BCC (2.5 M) 371 μl of ammonium sulfate BCC (3.5 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 448 μl of water (100 v/v) |
A8 |
| NA | 600 μl of cesium chloride BCC (5 M) 400 μl of water (100 v/v) |
A9 |
| NA | 285 μl of diammonium hydrogen phosphate BCC (3.5 M) 714 μl of water (100 v/v) |
A10 |
| pH 5 | 285 μl of diammonium hydrogen phosphate BCC (3.5 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 614 μl of water (100 v/v) |
A11 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 285 μl of diammonium hydrogen phosphate BCC (3.5 M) 40 μl of sodium chloride BCC (5 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 574 μl of water (100 v/v) |
A12 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 285 μl of diammonium hydrogen phosphate BCC (3.5 M) 120 μl of trehalose BCC (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 494 μl of water (100 v/v) |
B1 |
| pH 8 | 285 μl of diammonium hydrogen phosphate BCC (3.5 M) 40 μl of sodium chloride BCC (5 M) 100 μl of imidazole BCC pH 8 (1 M) 574 μl of water (100 v/v) |
B2 |
| NA | 500 μl of diammonium hydrogen phosphate BCC (3.5 M) 500 μl of water (100 v/v) |
B3 |
| pH 8.5 | 10 μl of sodium tetraborate BCC pH 8.5 (1 M) 375 μl of diammonium tartrate BCC (2 M) 615 μl of water (100 v/v) |
B4 |
| NA | 550 μl of diammonium tartrate BCC (2 M) 450 μl of water (100 v/v) |
B5 |
| pH 8.5 | 10 μl of tris chloride BCC pH 7 (1 M) 39 μl of tris chloride BCC pH 9 (1 M) 166 μl of dipotassium hydrogen phosphate BCC (3 M) 500 μl of potassium dihydrogen phosphate BCC (1 M) 283 μl of water (100 v/v) |
B6 |
| NA | 116 μl of sodium dihydrogen phosphate BCC (3 M) 216 μl of dipotassium hydrogen phosphate BCC (3 M) 666 μl of water (100 v/v) |
B7 |
| NA | 10 μl of sodium dihydrogen phosphate BCC (3 M) 256 μl of dipotassium hydrogen phosphate BCC (3 M) 733 μl of water (100 v/v) |
B8 |
| NA | 163 μl of sodium dihydrogen phosphate BCC (3 M) 303 μl of dipotassium hydrogen phosphate BCC (3 M) 533 μl of water (100 v/v) |
B9 |
| NA | 13 μl of sodium dihydrogen phosphate BCC (3 M) 320 μl of dipotassium hydrogen phosphate BCC (3 M) 666 μl of water (100 v/v) |
B10 |
| NA | 333 μl of dipotassium hydrogen phosphate BCC (3 M) 500 μl of potassium dihydrogen phosphate BCC (1 M) 166 μl of water (100 v/v) |
B11 |
| NA | 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 500 μl of disodium hydrogen phosphate BCC pH 9.3 (1 M) 333 μl of dipotassium hydrogen phosphate BCC (3 M) 66 μl of water (100 v/v) |
B12 |
| pH 5 | 266 μl of sodium dihydrogen phosphate BCC (3 M) 400 μl of dipotassium hydrogen phosphate BCC (3 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 233 μl of water (100 v/v) |
C1 |
| NA | 18 μl of sodium dihydrogen phosphate BCC (3 M) 448 μl of dipotassium hydrogen phosphate BCC (3 M) 533 μl of water (100 v/v) |
C2 |
| NA | 24 μl of sodium dihydrogen phosphate BCC (3 M) 576 μl of dipotassium hydrogen phosphate BCC (3 M) 400 μl of water (100 v/v) |
C3 |
| NA | 800 μl of lithium acetate BCC (2.5 M) 200 μl of water (100 v/v) |
C4 |
| NA | 200 μl of lithium chloride BCC (10 M) 10 μl of tergitol NP-40 (1 w/v) 790 μl of water (100 v/v) |
C5 |
| NA | 250 μl of lithium chloride BCC (10 M) 57 μl of ammonium sulfate BCC (3.5 M) 692 μl of water (100 v/v) |
C6 |
| pH 6.5 | 83 μl of imidazole BCC pH 6 (1 M) 285 μl of lithium formate BCC (3.5 M) 16 μl of imidazole BCC pH 8 (1 M) 614 μl of water (100 v/v) |
C7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 120 μl of trehalose BCC (1 M) 600 μl of magnesium acetate BCC (2.5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 180 μl of water (100 v/v) |
C8 |
| pH 8.5 | 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 600 μl of magnesium acetate BCC (2.5 M) 200 μl of lithium sulfate BCC (1 M) 100 μl of water (100 v/v) |
C9 |
| pH 4.5 | 200 μl of magnesium chloride BCC (2.5 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 700 μl of water (100 v/v) |
C10 |
| NA | 333 μl of potassium chloride BCC (3 M) 400 μl of magnesium chloride BCC (2.5 M) 266 μl of water (100 v/v) |
C11 |
| pH 9 | 800 μl of magnesium chloride BCC (2.5 M) 12 μl of sodium bicine BCC pH 7.4 (1 M) 87 μl of sodium bicine BCC pH 9.4 (1 M) 100 μl of water (100 v/v) |
C12 |
| NA | 200 μl of magnesium formate BCC (1 M) 800 μl of water (100 v/v) |
D1 |
| pH 5.5 | 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 300 μl of magnesium formate BCC (1 M) 600 μl of water (100 v/v) |
D2 |
| pH 8.5 | 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 300 μl of magnesium formate BCC (1 M) 600 μl of water (100 v/v) |
D3 |
| pH 6.5 | 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 500 μl of magnesium formate BCC (1 M) 400 μl of water (100 v/v) |
D4 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of magnesium formate BCC (1 M) 400 μl of water (100 v/v) |
D5 |
| pH 5.6 | 9 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500 μl of potassium dihydrogen phosphate BCC (1 M) 41 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 450 μl of water (100 v/v) |
D6 |
| NA | 25 μl of 1-octanol BCC (100 v/v) 200 μl of potassium formate BCC (5 M) 775 μl of water (100 v/v) |
D7 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of potassium sodium tartrate BCC (2 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 500 μl of water (100 v/v) |
D8 |
| pH 8.5 | 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 25 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 400 μl of potassium sodium tartrate BCC (2 M) 475 μl of water (100 v/v) |
D9 |
| NA | 500 μl of potassium sodium tartrate BCC (2 M) 500 μl of water (100 v/v) |
D10 |
| NA | 500 μl of potassium sodium tartrate BCC (2 M) 500 μl of diammonium tartrate BCC (2 M) |
D11 |
| pH 7 | 100 μl of tris chloride BCC pH 7 (1 M) 500 μl of potassium sodium tartrate BCC (2 M) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
D12 |
| pH 8 | 40 μl of sodium chloride BCC (5 M) 500 μl of potassium sodium tartrate BCC (2 M) 100 μl of imidazole BCC pH 8 (1 M) 360 μl of water (100 v/v) |
E1 |
| pH 9.5 | 79 μl of CHES BCC pH 10 (0.8 M) 45 μl of CHES BCC pH 8.3 (0.8 M) 500 μl of potassium sodium tartrate BCC (2 M) 200 μl of lithium sulfate BCC (1 M) 175 μl of water (100 v/v) |
E2 |
| pH 7.5 | 25 μl of sodium HEPES BCC pH 8.5 (1 M) 700 μl of potassium sodium tartrate BCC (2 M) 30 μl of trehalose BCC (1 M) 25 μl of sodium HEPES BCC pH 6.5 (1 M) 220 μl of water (100 v/v) |
E3 |
| pH 6.5 | 65 μl of sodium cacodylate BCC pH 7.3 (1 M) 34 μl of sodium cacodylate BCC pH 5.3 (1 M) 466 μl of sodium acetate BCC (3 M) 433 μl of water (100 v/v) |
E4 |
| pH 6.5 | 120 μl of trehalose BCC (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 466 μl of sodium acetate BCC (3 M) 313 μl of water (100 v/v) |
E5 |
| NA | 933 μl of sodium acetate BCC (3 M) 66 μl of water (100 v/v) |
E6 |
| NA | 100 μl of lithium chloride BCC (10 M) 200 μl of sodium chloride BCC (5 M) 700 μl of water (100 v/v) |
E7 |
| pH 9.4 | 280 μl of sodium chloride BCC (5 M) 100 μl of sodium bicine BCC pH 9.4 (1 M) 620 μl of water (100 v/v) |
E8 |
| NA | 300 μl of sodium chloride BCC (5 M) 100 μl of ethanol BCC (100 v/v) 600 μl of water (100 v/v) |
E9 |
| NA | 200 μl of polyethylene glycol 6000 BCC (50 w/v) 400 μl of sodium chloride BCC (5 M) 117 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 282 μl of water (100 v/v) |
E10 |
| NA | 200 μl of polyethylene glycol 6000 BCC (50 w/v) 400 μl of sodium chloride BCC (5 M) 400 μl of water (100 v/v) |
E11 |
| pH 4.5 | 400 μl of sodium chloride BCC (5 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 125 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 375 μl of water (100 v/v) |
E12 |
| pH 6.5 | 33 μl of sodium dihydrogen phosphate BCC (3 M) 400 μl of sodium chloride BCC (5 M) 100 μl of potassium dihydrogen phosphate BCC (1 M) 27 μl of sodium MES BCC pH 5.2 (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 366 μl of water (100 v/v) |
F1 |
| pH 6.5 | 400 μl of sodium chloride BCC (5 M) 120 μl of trehalose BCC (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 27 μl of sodium MES BCC pH 5.2 (1 M) 72 μl of sodium MES BCC pH 7.2 (1 M) 330 μl of water (100 v/v) |
F2 |
| pH 7.5 | 125 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 375 μl of water (100 v/v) |
F3 |
| pH 7.5 | 125 μl of polyethylene glycol 400 BCC (80 v/v) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 400 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 375 μl of water (100 v/v) |
F4 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 400 μl of sodium chloride BCC (5 M) 82 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 417 μl of water (100 v/v) |
F5 |
| NA | 33 μl of sodium dihydrogen phosphate BCC (3 M) 500 μl of sodium chloride BCC (5 M) 466 μl of water (100 v/v) |
F6 |
| pH 5 | 500 μl of sodium chloride BCC (5 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 200 μl of lithium sulfate BCC (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 200 μl of water (100 v/v) |
F7 |
| pH 7 | 100 μl of tris chloride BCC pH 7 (1 M) 80 μl of magnesium chloride BCC (2.5 M) 500 μl of sodium chloride BCC (5 M) 320 μl of water (100 v/v) |
F8 |
| pH 8 | 54 μl of tris chloride BCC pH 7 (1 M) 45 μl of tris chloride BCC pH 9 (1 M) 500 μl of sodium chloride BCC (5 M) 20 μl of magnesium nitrate BCC (1 M) 380 μl of water (100 v/v) |
F9 |
| pH 8 | 500 μl of sodium chloride BCC (5 M) 100 μl of imidazole BCC pH 8 (1 M) 400 μl of water (100 v/v) |
F10 |
| pH 3.5 | 70 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 600 μl of sodium chloride BCC (5 M) 30 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 300 μl of water (100 v/v) |
F11 |
| pH 4.5 | 600 μl of sodium chloride BCC (5 M) 31 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 300 μl of water (100 v/v) |
F12 |
| pH 4.5 | 600 μl of sodium chloride BCC (5 M) 166 μl of n-octyl-b-D-Glucopyranoside BCC (3 w/v) 18 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 41 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 173 μl of water (100 v/v) |
G1 |
| pH 5.5 | 600 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 5.5 (1 M) 300 μl of water (100 v/v) |
G2 |
| pH 6.5 | 600 μl of sodium chloride BCC (5 M) 100 μl of bis-tris chloride BCC pH 6.5 (1 M) 300 μl of water (100 v/v) |
G3 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 600 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 300 μl of water (100 v/v) |
G4 |
| pH 8.5 | 21 μl of tris chloride BCC pH 7 (1 M) 78 μl of tris chloride BCC pH 9 (1 M) 600 μl of sodium chloride BCC (5 M) 300 μl of water (100 v/v) |
G5 |
| pH 8.5 | 10 μl of tris chloride BCC pH 7 (1 M) 39 μl of tris chloride BCC pH 9 (1 M) 600 μl of sodium chloride BCC (5 M) 120 μl of trehalose BCC (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 180 μl of water (100 v/v) |
G6 |
| pH 7.5 | 50 μl of sodium HEPES BCC pH 8.5 (1 M) 860 μl of sodium chloride BCC (5 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 40 μl of water (100 v/v) |
G7 |
| pH 8 | 133 μl of sodium dihydrogen phosphate BCC (3 M) 40 μl of sodium chloride BCC (5 M) 100 μl of imidazole BCC pH 8 (1 M) 726 μl of water (100 v/v) |
G8 |
| NA | 173 μl of sodium dihydrogen phosphate BCC (3 M) 93 μl of dipotassium hydrogen phosphate BCC (3 M) 733 μl of water (100 v/v) |
G9 |
| pH 5.5 | 20 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 240 μl of sodium dihydrogen phosphate BCC (3 M) 26 μl of dipotassium hydrogen phosphate BCC (3 M) 80 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 633 μl of water (100 v/v) |
G10 |
| pH 7.5 | 320 μl of ammonium dihydrogen phosphate BCC (2.5 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 266 μl of sodium dihydrogen phosphate BCC (3 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 313 μl of water (100 v/v) |
G11 |
| NA | 303 μl of sodium dihydrogen phosphate BCC (3 M) 163 μl of dipotassium hydrogen phosphate BCC (3 M) 533 μl of water (100 v/v) |
G12 |
| NA | 326 μl of sodium dihydrogen phosphate BCC (3 M) 6 μl of dipotassium hydrogen phosphate BCC (3 M) 666 μl of water (100 v/v) |
H1 |
| NA | 390 μl of sodium dihydrogen phosphate BCC (3 M) 210 μl of dipotassium hydrogen phosphate BCC (3 M) 400 μl of water (100 v/v) |
H2 |
| pH 10 | 114 μl of CAPS BCC pH 11 (0.5 M) 400 μl of sodium dihydrogen phosphate BCC (3 M) 86 μl of CAPS BCC pH 9.4 (0.5 M) 200 μl of lithium sulfate BCC (1 M) 200 μl of water (100 v/v) |
H3 |
| NA | 457 μl of sodium dihydrogen phosphate BCC (3 M) 9 μl of dipotassium hydrogen phosphate BCC (3 M) 533 μl of water (100 v/v) |
H4 |
| pH 4.2 | 533 μl of sodium dihydrogen phosphate BCC (3 M) 133 μl of dipotassium hydrogen phosphate BCC (3 M) 112 μl of phosphate-citrate BCC pH 9.3 (1 M) 88 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 133 μl of water (100 v/v) |
H5 |
| pH 5 | 285 μl of sodium formate BCC (7 M) 65 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 614 μl of water (100 v/v) |
H6 |
| pH 6.5 | 285 μl of sodium formate BCC (7 M) 54 μl of ADA BCC pH 5.5 (0.5 M) 45 μl of ADA BCC pH 7.5 (0.5 M) 614 μl of water (100 v/v) |
H7 |
| pH 7.5 | 428 μl of sodium formate BCC (7 M) 50 μl of sodium HEPES BCC pH 8.5 (1 M) 50 μl of sodium HEPES BCC pH 6.5 (1 M) 471 μl of water (100 v/v) |
H8 |
| NA | 500 μl of sodium formate BCC (7 M) 100 μl of zinc sulfate BCC (0.1 M) 400 μl of water (100 v/v) |
H9 |
| NA | 571 μl of sodium formate BCC (7 M) 428 μl of water (100 v/v) |
H10 |
| pH 8.5 | 10 μl of tris chloride BCC pH 7 (1 M) 39 μl of tris chloride BCC pH 9 (1 M) 571 μl of sodium formate BCC (7 M) 378 μl of water (100 v/v) |
H11 |
| NA | 400 μl of sodium chloride BCC (5 M) 400 μl of trichloroacetic acid BCC (100 w/v) 200 μl of water (100 v/v) |
H12 |
| pH 7 | 100 μl of tris chloride BCC pH 7 (1 M) 625 μl of trisodium citrate BCC (1.6 M) 40 μl of sodium chloride BCC (5 M) 235 μl of water (100 v/v) |