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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
c3_6
BCC Core Screens / c3_6
Created 2025-03-06.
Salty: various phosphates, chlorides, acetates, formates, tartrates
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c3_6
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c3_6
Screen Structure
c3_6
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.946 |
Screen Composition
ingredient type | c3_6 |
---|---|
Buffer | 84 |
Cryoprotectant | 6 |
Detergent | 2 |
Organic (non-volatile) | 8 |
Organic (volatile) | 3 |
Polymer | 8 |
Salt | 142 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 160.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 840.0 μl of water (100 v/v) |
A2 |
| pH 5.5 | 400.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 500.0 μl of water (100 v/v) |
A3 |
| NA | 600.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 400.0 μl of water (100 v/v) |
A4 |
| pH 8.5 | 800.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
A5 |
| pH 9 | 37.1 μl of CHES BCC pH 10 (0.8 M) 87.9 μl of CHES BCC pH 8.3 (0.8 M) 375.0 μl of ammonium formate BCC (4 M) 120.0 μl of magnesium chloride BCC (2.5 M) 380.0 μl of water (100 v/v) |
A6 |
| pH 7.5 | 500.0 μl of ammonium formate BCC (4 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
A7 |
| pH 6.5 | 80.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 371.4 μl of ammonium sulfate BCC (3.5 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 448.6 μl of water (100 v/v) |
A8 |
| NA | 600.0 μl of cesium chloride BCC (5 M) 400.0 μl of water (100 v/v) |
A9 |
| NA | 285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 714.3 μl of water (100 v/v) |
A10 |
| pH 5 | 285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 614.3 μl of water (100 v/v) |
A11 |
| pH 5.5 | 285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 40.0 μl of sodium chloride BCC (5 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 574.3 μl of water (100 v/v) |
A12 |
| pH 7.5 | 285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 120.0 μl of trehalose BCC (1 M) 494.3 μl of water (100 v/v) |
B1 |
| pH 8 | 285.7 μl of diammonium hydrogen phosphate BCC (3.5 M) 100.0 μl of imidazole BCC pH 8 (1 M) 40.0 μl of sodium chloride BCC (5 M) 574.3 μl of water (100 v/v) |
B2 |
| NA | 500.0 μl of diammonium hydrogen phosphate BCC (3.5 M) 500.0 μl of water (100 v/v) |
B3 |
| pH 8.5 | 375.0 μl of diammonium tartrate BCC (2 M) 10.0 μl of sodium tetraborate BCC pH 8.5 (1 M) 615.0 μl of water (100 v/v) |
B4 |
| NA | 550.0 μl of diammonium tartrate BCC (2 M) 450.0 μl of water (100 v/v) |
B5 |
| pH 8.5 | 166.7 μl of dipotassium hydrogen phosphate BCC (3 M) 500.0 μl of potassium dihydrogen phosphate BCC (1 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 283.3 μl of water (100 v/v) |
B6 |
| NA | 216.7 μl of dipotassium hydrogen phosphate BCC (3 M) 116.7 μl of sodium dihydrogen phosphate BCC (3 M) 666.7 μl of water (100 v/v) |
B7 |
| NA | 256.0 μl of dipotassium hydrogen phosphate BCC (3 M) 10.7 μl of sodium dihydrogen phosphate BCC (3 M) 733.3 μl of water (100 v/v) |
B8 |
| NA | 303.3 μl of dipotassium hydrogen phosphate BCC (3 M) 163.3 μl of sodium dihydrogen phosphate BCC (3 M) 533.3 μl of water (100 v/v) |
B9 |
| NA | 320.0 μl of dipotassium hydrogen phosphate BCC (3 M) 13.3 μl of sodium dihydrogen phosphate BCC (3 M) 666.7 μl of water (100 v/v) |
B10 |
| NA | 333.3 μl of dipotassium hydrogen phosphate BCC (3 M) 500.0 μl of potassium dihydrogen phosphate BCC (1 M) 166.7 μl of water (100 v/v) |
B11 |
| NA | 333.3 μl of dipotassium hydrogen phosphate BCC (3 M) 500.0 μl of disodium hydrogen phosphate BCC pH 9.3 (1 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 66.7 μl of water (100 v/v) |
B12 |
| pH 5 | 400.0 μl of dipotassium hydrogen phosphate BCC (3 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 266.7 μl of sodium dihydrogen phosphate BCC (3 M) 233.3 μl of water (100 v/v) |
C1 |
| NA | 448.0 μl of dipotassium hydrogen phosphate BCC (3 M) 18.7 μl of sodium dihydrogen phosphate BCC (3 M) 533.3 μl of water (100 v/v) |
C2 |
| NA | 576.0 μl of dipotassium hydrogen phosphate BCC (3 M) 24.0 μl of sodium dihydrogen phosphate BCC (3 M) 400.0 μl of water (100 v/v) |
C3 |
| NA | 800.0 μl of lithium acetate BCC (2.5 M) 200.0 μl of water (100 v/v) |
C4 |
| NA | 200.0 μl of lithium chloride BCC (10 M) 10.0 μl of tergitol NP-40 (1 w/v) 790.0 μl of water (100 v/v) |
C5 |
| NA | 57.1 μl of ammonium sulfate BCC (3.5 M) 250.0 μl of lithium chloride BCC (10 M) 692.9 μl of water (100 v/v) |
C6 |
| pH 6.5 | 83.1 μl of imidazole BCC pH 6 (1 M) 16.9 μl of imidazole BCC pH 8 (1 M) 285.7 μl of lithium formate BCC (3.5 M) 614.3 μl of water (100 v/v) |
C7 |
| pH 7.5 | 600.0 μl of magnesium acetate BCC (2.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 120.0 μl of trehalose BCC (1 M) 180.0 μl of water (100 v/v) |
C8 |
| pH 8.5 | 200.0 μl of lithium sulfate BCC (1 M) 600.0 μl of magnesium acetate BCC (2.5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 100.0 μl of water (100 v/v) |
C9 |
| pH 4.5 | 200.0 μl of magnesium chloride BCC (2.5 M) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 700.0 μl of water (100 v/v) |
C10 |
| NA | 400.0 μl of magnesium chloride BCC (2.5 M) 333.3 μl of potassium chloride BCC (3 M) 266.7 μl of water (100 v/v) |
C11 |
| pH 9 | 800.0 μl of magnesium chloride BCC (2.5 M) 12.4 μl of sodium bicine BCC pH 7.4 (1 M) 87.6 μl of sodium bicine BCC pH 9.4 (1 M) 100.0 μl of water (100 v/v) |
C12 |
| NA | 200.0 μl of magnesium formate BCC (1 M) 800.0 μl of water (100 v/v) |
D1 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 300.0 μl of magnesium formate BCC (1 M) 600.0 μl of water (100 v/v) |
D2 |
| pH 8.5 | 300.0 μl of magnesium formate BCC (1 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 600.0 μl of water (100 v/v) |
D3 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 500.0 μl of magnesium formate BCC (1 M) 400.0 μl of water (100 v/v) |
D4 |
| pH 7.5 | 500.0 μl of magnesium formate BCC (1 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D5 |
| pH 5.6 | 500.0 μl of potassium dihydrogen phosphate BCC (1 M) 41.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 9.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 450.0 μl of water (100 v/v) |
D6 |
| NA | 25.0 μl of 1-octanol BCC (100 v/v) 200.0 μl of potassium formate BCC (5 M) 775.0 μl of water (100 v/v) |
D7 |
| pH 7.5 | 400.0 μl of potassium sodium tartrate BCC (2 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
D8 |
| pH 8.5 | 25.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v) 400.0 μl of potassium sodium tartrate BCC (2 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 475.0 μl of water (100 v/v) |
D9 |
| NA | 500.0 μl of potassium sodium tartrate BCC (2 M) 500.0 μl of water (100 v/v) |
D10 |
| NA | 500.0 μl of diammonium tartrate BCC (2 M) 500.0 μl of potassium sodium tartrate BCC (2 M) |
D11 |
| pH 7 | 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of potassium sodium tartrate BCC (2 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 200.0 μl of water (100 v/v) |
D12 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 500.0 μl of potassium sodium tartrate BCC (2 M) 40.0 μl of sodium chloride BCC (5 M) 360.0 μl of water (100 v/v) |
E1 |
| pH 9.5 | 79.8 μl of CHES BCC pH 10 (0.8 M) 45.2 μl of CHES BCC pH 8.3 (0.8 M) 200.0 μl of lithium sulfate BCC (1 M) 500.0 μl of potassium sodium tartrate BCC (2 M) 175.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 700.0 μl of potassium sodium tartrate BCC (2 M) 25.0 μl of sodium HEPES BCC pH 6.5 (1 M) 25.0 μl of sodium HEPES BCC pH 8.5 (1 M) 30.0 μl of trehalose BCC (1 M) 220.0 μl of water (100 v/v) |
E3 |
| pH 6.5 | 466.7 μl of sodium acetate BCC (3 M) 34.9 μl of sodium cacodylate BCC pH 5.3 (1 M) 65.1 μl of sodium cacodylate BCC pH 7.3 (1 M) 433.3 μl of water (100 v/v) |
E4 |
| pH 6.5 | 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 466.7 μl of sodium acetate BCC (3 M) 120.0 μl of trehalose BCC (1 M) 313.3 μl of water (100 v/v) |
E5 |
| NA | 933.3 μl of sodium acetate BCC (3 M) 66.7 μl of water (100 v/v) |
E6 |
| NA | 100.0 μl of lithium chloride BCC (10 M) 200.0 μl of sodium chloride BCC (5 M) 700.0 μl of water (100 v/v) |
E7 |
| pH 9.4 | 100.0 μl of sodium bicine BCC pH 9.4 (1 M) 280.0 μl of sodium chloride BCC (5 M) 620.0 μl of water (100 v/v) |
E8 |
| NA | 100.0 μl of ethanol BCC (100 v/v) 300.0 μl of sodium chloride BCC (5 M) 600.0 μl of water (100 v/v) |
E9 |
| NA | 200.0 μl of polyethylene glycol 6000 BCC (50 w/v) 117.6 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 400.0 μl of sodium chloride BCC (5 M) 282.4 μl of water (100 v/v) |
E10 |
| NA | 200.0 μl of polyethylene glycol 6000 BCC (50 w/v) 400.0 μl of sodium chloride BCC (5 M) 400.0 μl of water (100 v/v) |
E11 |
| pH 4.5 | 125.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v) 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 400.0 μl of sodium chloride BCC (5 M) 375.0 μl of water (100 v/v) |
E12 |
| pH 6.5 | 100.0 μl of potassium dihydrogen phosphate BCC (1 M) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 400.0 μl of sodium chloride BCC (5 M) 33.3 μl of sodium dihydrogen phosphate BCC (3 M) 366.7 μl of water (100 v/v) |
F1 |
| pH 6.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 27.8 μl of sodium MES BCC pH 5.2 (1 M) 72.2 μl of sodium MES BCC pH 7.2 (1 M) 400.0 μl of sodium chloride BCC (5 M) 120.0 μl of trehalose BCC (1 M) 330.0 μl of water (100 v/v) |
F2 |
| pH 7.5 | 125.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of sodium chloride BCC (5 M) 375.0 μl of water (100 v/v) |
F3 |
| pH 7.5 | 125.0 μl of polyethylene glycol 400 BCC (80 v/v) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 400.0 μl of sodium chloride BCC (5 M) 375.0 μl of water (100 v/v) |
F4 |
| pH 8 | 82.4 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v) 400.0 μl of sodium chloride BCC (5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 417.6 μl of water (100 v/v) |
F5 |
| NA | 500.0 μl of sodium chloride BCC (5 M) 33.3 μl of sodium dihydrogen phosphate BCC (3 M) 466.7 μl of water (100 v/v) |
F6 |
| pH 5 | 200.0 μl of lithium sulfate BCC (1 M) 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 500.0 μl of sodium chloride BCC (5 M) 200.0 μl of water (100 v/v) |
F7 |
| pH 7 | 80.0 μl of magnesium chloride BCC (2.5 M) 500.0 μl of sodium chloride BCC (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 320.0 μl of water (100 v/v) |
F8 |
| pH 8 | 20.0 μl of magnesium nitrate BCC (1 M) 500.0 μl of sodium chloride BCC (5 M) 54.7 μl of tris chloride BCC pH 7 (1 M) 45.3 μl of tris chloride BCC pH 9 (1 M) 380.0 μl of water (100 v/v) |
F9 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 500.0 μl of sodium chloride BCC (5 M) 400.0 μl of water (100 v/v) |
F10 |
| pH 3.5 | 600.0 μl of sodium chloride BCC (5 M) 30.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 70.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 300.0 μl of water (100 v/v) |
F11 |
| pH 4.5 | 31.3 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 68.7 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 600.0 μl of sodium chloride BCC (5 M) 300.0 μl of water (100 v/v) |
F12 |
| pH 4.5 | 166.7 μl of n-octyl-b-D-Glucopyranoside BCC (3 w/v) 18.8 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 41.2 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 600.0 μl of sodium chloride BCC (5 M) 173.3 μl of water (100 v/v) |
G1 |
| pH 5.5 | 100.0 μl of bis-tris chloride BCC pH 5.5 (1 M) 600.0 μl of sodium chloride BCC (5 M) 300.0 μl of water (100 v/v) |
G2 |
| pH 6.5 | 100.0 μl of bis-tris chloride BCC pH 6.5 (1 M) 600.0 μl of sodium chloride BCC (5 M) 300.0 μl of water (100 v/v) |
G3 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 600.0 μl of sodium chloride BCC (5 M) 300.0 μl of water (100 v/v) |
G4 |
| pH 8.5 | 600.0 μl of sodium chloride BCC (5 M) 21.2 μl of tris chloride BCC pH 7 (1 M) 78.8 μl of tris chloride BCC pH 9 (1 M) 300.0 μl of water (100 v/v) |
G5 |
| pH 8.5 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 600.0 μl of sodium chloride BCC (5 M) 120.0 μl of trehalose BCC (1 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 180.0 μl of water (100 v/v) |
G6 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 860.0 μl of sodium chloride BCC (5 M) 40.0 μl of water (100 v/v) |
G7 |
| pH 8 | 100.0 μl of imidazole BCC pH 8 (1 M) 40.0 μl of sodium chloride BCC (5 M) 133.3 μl of sodium dihydrogen phosphate BCC (3 M) 726.7 μl of water (100 v/v) |
G8 |
| NA | 93.3 μl of dipotassium hydrogen phosphate BCC (3 M) 173.3 μl of sodium dihydrogen phosphate BCC (3 M) 733.3 μl of water (100 v/v) |
G9 |
| pH 5.5 | 26.7 μl of dipotassium hydrogen phosphate BCC (3 M) 240.0 μl of sodium dihydrogen phosphate BCC (3 M) 80.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M) 20.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M) 633.3 μl of water (100 v/v) |
G10 |
| pH 7.5 | 320.0 μl of ammonium dihydrogen phosphate BCC (2.5 M) 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 266.7 μl of sodium dihydrogen phosphate BCC (3 M) 313.3 μl of water (100 v/v) |
G11 |
| NA | 163.3 μl of dipotassium hydrogen phosphate BCC (3 M) 303.3 μl of sodium dihydrogen phosphate BCC (3 M) 533.3 μl of water (100 v/v) |
G12 |
| NA | 6.7 μl of dipotassium hydrogen phosphate BCC (3 M) 326.7 μl of sodium dihydrogen phosphate BCC (3 M) 666.7 μl of water (100 v/v) |
H1 |
| NA | 210.0 μl of dipotassium hydrogen phosphate BCC (3 M) 390.0 μl of sodium dihydrogen phosphate BCC (3 M) 400.0 μl of water (100 v/v) |
H2 |
| pH 10 | 86.0 μl of CAPS BCC pH 9.4 (0.5 M) 114.0 μl of CAPS BCC pH 11 (0.5 M) 200.0 μl of lithium sulfate BCC (1 M) 400.0 μl of sodium dihydrogen phosphate BCC (3 M) 200.0 μl of water (100 v/v) |
H3 |
| NA | 9.3 μl of dipotassium hydrogen phosphate BCC (3 M) 457.3 μl of sodium dihydrogen phosphate BCC (3 M) 533.3 μl of water (100 v/v) |
H4 |
| pH 4.2 | 133.3 μl of dipotassium hydrogen phosphate BCC (3 M) 88.0 μl of phosphate-citrate BCC pH 2.2 (0.5 M) 112.0 μl of phosphate-citrate BCC pH 9.3 (1 M) 533.3 μl of sodium dihydrogen phosphate BCC (3 M) 133.3 μl of water (100 v/v) |
H5 |
| pH 5 | 65.5 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M) 34.5 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M) 285.7 μl of sodium formate BCC (7 M) 614.3 μl of water (100 v/v) |
H6 |
| pH 6.5 | 54.7 μl of ADA BCC pH 5.5 (0.5 M) 45.3 μl of ADA BCC pH 7.5 (0.5 M) 285.7 μl of sodium formate BCC (7 M) 614.3 μl of water (100 v/v) |
H7 |
| pH 7.5 | 50.0 μl of sodium HEPES BCC pH 6.5 (1 M) 50.0 μl of sodium HEPES BCC pH 8.5 (1 M) 428.6 μl of sodium formate BCC (7 M) 471.4 μl of water (100 v/v) |
H8 |
| NA | 500.0 μl of sodium formate BCC (7 M) 100.0 μl of zinc sulfate BCC (0.1 M) 400.0 μl of water (100 v/v) |
H9 |
| NA | 571.4 μl of sodium formate BCC (7 M) 428.6 μl of water (100 v/v) |
H10 |
| pH 8.5 | 571.4 μl of sodium formate BCC (7 M) 10.6 μl of tris chloride BCC pH 7 (1 M) 39.4 μl of tris chloride BCC pH 9 (1 M) 378.6 μl of water (100 v/v) |
H11 |
| NA | 400.0 μl of sodium chloride BCC (5 M) 400.0 μl of trichloroacetic acid BCC (100 w/v) 200.0 μl of water (100 v/v) |
H12 |
| pH 7 | 40.0 μl of sodium chloride BCC (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 625.0 μl of trisodium citrate BCC (1.6 M) 235.0 μl of water (100 v/v) |