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Supplemental Information

Screen Description

Created 2025-03-06.
Salty: various phosphates, chlorides, acetates, formates, tartrates

Rigaku Recipe XML

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Barcodes

  • Z0002645 Lot 1 (1500.0 µl)
  • Z0002646 Lot 2 (1500.0 µl)
  • Z0002710 Lot 3 (1500.0 µl)
  • Z0002711 Lot 4 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 52
Internal diversity (Score mean dist)0.94
Internal diversity (Ion mean dist)0.9
Internal diversity (PEG mean dist)0.9
Internal diversity (Known pH mean dist)0.86
Internal diversity (Penalty mean dist)0.91

Screen Composition

Chemical classes bar chart
ingredient type c3_6
Buffer 84
Cryoprotectant 6
Detergent 2
Organic (non-volatile) 8
Organic (volatile) 3
Polymer 8
Salt 142

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
c3_6 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • ammonium dihydrogen phosphate BCC 0.4 M -
NA
160.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
840.0 μl of water (100 v/v)
A2
  • ammonium dihydrogen phosphate BCC 1 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
400.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
500.0 μl of water (100 v/v)
A3
  • ammonium dihydrogen phosphate BCC 1.5 M -
NA
600.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
400.0 μl of water (100 v/v)
A4
  • ammonium dihydrogen phosphate BCC 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
800.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
A5
  • ammonium formate BCC 1.5 M -
  • magnesium chloride BCC 0.3 M -
  • CHES BCC 0.1 M pH 9
pH 9
375.0 μl of ammonium formate BCC (4 M)
37.12 μl of CHES BCC pH 10.3 (0.8 M)
87.88 μl of CHES BCC pH 8.3 (0.8 M)
120.0 μl of magnesium chloride BCC (2.5 M)
380.0 μl of water (100 v/v)
A6
  • ammonium formate BCC 2 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of ammonium formate BCC (4 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
A7
  • ammonium sulfate BCC 1.3 M -
  • ammonium dihydrogen phosphate BCC 0.2 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
80.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
371.43 μl of ammonium sulfate BCC (3.5 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
448.57 μl of water (100 v/v)
A8
  • cesium chloride BCC 3 M -
NA
600.0 μl of cesium chloride BCC (5 M)
400.0 μl of water (100 v/v)
A9
  • diammonium hydrogen phosphate BCC 1 M -
NA
285.71 μl of diammonium hydrogen phosphate BCC (3.5 M)
714.29 μl of water (100 v/v)
A10
  • diammonium hydrogen phosphate BCC 1 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
285.71 μl of diammonium hydrogen phosphate BCC (3.5 M)
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
614.29 μl of water (100 v/v)
A11
  • diammonium hydrogen phosphate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
285.71 μl of diammonium hydrogen phosphate BCC (3.5 M)
40.0 μl of sodium chloride BCC (5 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
574.29 μl of water (100 v/v)
A12
  • diammonium hydrogen phosphate BCC 1 M -
  • trehalose BCC 0.12 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
285.71 μl of diammonium hydrogen phosphate BCC (3.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
120.0 μl of trehalose BCC (1 M)
494.29 μl of water (100 v/v)
B1
  • diammonium hydrogen phosphate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
pH 8
285.71 μl of diammonium hydrogen phosphate BCC (3.5 M)
100.0 μl of imidazole BCC pH 8 (1 M)
40.0 μl of sodium chloride BCC (5 M)
574.29 μl of water (100 v/v)
B2
  • diammonium hydrogen phosphate BCC 1.75 M -
NA
500.0 μl of diammonium hydrogen phosphate BCC (3.5 M)
500.0 μl of water (100 v/v)
B3
  • diammonium tartrate BCC 0.75 M -
  • sodium tetraborate BCC 0.01 M pH 8.5
pH 8.5
375.0 μl of diammonium tartrate BCC (2 M)
10.0 μl of sodium tetraborate BCC pH 8.5 (1 M)
615.0 μl of water (100 v/v)
B4
  • diammonium tartrate BCC 1.1 M -
NA
550.0 μl of diammonium tartrate BCC (2 M)
450.0 μl of water (100 v/v)
B5
  • dipotassium hydrogen phosphate BCC 0.5 M -
  • potassium dihydrogen phosphate BCC 0.5 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
166.67 μl of dipotassium hydrogen phosphate BCC (3 M)
500.0 μl of potassium dihydrogen phosphate BCC (1 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
283.33 μl of water (100 v/v)
B6
  • dipotassium hydrogen phosphate BCC 0.65 M -
  • sodium dihydrogen phosphate BCC 0.35 M -
NA
216.67 μl of dipotassium hydrogen phosphate BCC (3 M)
116.67 μl of sodium dihydrogen phosphate BCC (3 M)
666.66 μl of water (100 v/v)
B7
  • dipotassium hydrogen phosphate BCC 0.768 M -
  • sodium dihydrogen phosphate BCC 0.032 M -
NA
256.0 μl of dipotassium hydrogen phosphate BCC (3 M)
10.67 μl of sodium dihydrogen phosphate BCC (3 M)
733.33 μl of water (100 v/v)
B8
  • dipotassium hydrogen phosphate BCC 0.91 M -
  • sodium dihydrogen phosphate BCC 0.49 M -
NA
303.33 μl of dipotassium hydrogen phosphate BCC (3 M)
163.33 μl of sodium dihydrogen phosphate BCC (3 M)
533.34 μl of water (100 v/v)
B9
  • dipotassium hydrogen phosphate BCC 0.96 M -
  • sodium dihydrogen phosphate BCC 0.04 M -
NA
320.0 μl of dipotassium hydrogen phosphate BCC (3 M)
13.33 μl of sodium dihydrogen phosphate BCC (3 M)
666.67 μl of water (100 v/v)
B10
  • dipotassium hydrogen phosphate BCC 1 M -
  • potassium dihydrogen phosphate BCC 0.5 M -
NA
333.33 μl of dipotassium hydrogen phosphate BCC (3 M)
500.0 μl of potassium dihydrogen phosphate BCC (1 M)
166.67 μl of water (100 v/v)
B11
  • dipotassium hydrogen phosphate BCC 1 M -
  • disodium hydrogen phosphate BCC 0.5 M pH 9.3
  • tris chloride BCC 0.1 M pH 8
NA
333.33 μl of dipotassium hydrogen phosphate BCC (3 M)
500.0 μl of disodium hydrogen phosphate BCC pH 9.3 (1 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
66.67 μl of water (100 v/v)
B12
  • dipotassium hydrogen phosphate BCC 1.2 M -
  • sodium dihydrogen phosphate BCC 0.8 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
400.0 μl of dipotassium hydrogen phosphate BCC (3 M)
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
266.67 μl of sodium dihydrogen phosphate BCC (3 M)
233.33 μl of water (100 v/v)
C1
  • dipotassium hydrogen phosphate BCC 1.34 M -
  • sodium dihydrogen phosphate BCC 0.056 M -
NA
448.0 μl of dipotassium hydrogen phosphate BCC (3 M)
18.67 μl of sodium dihydrogen phosphate BCC (3 M)
533.33 μl of water (100 v/v)
C2
  • dipotassium hydrogen phosphate BCC 1.73 M -
  • sodium dihydrogen phosphate BCC 0.072 M -
NA
576.0 μl of dipotassium hydrogen phosphate BCC (3 M)
24.0 μl of sodium dihydrogen phosphate BCC (3 M)
400.0 μl of water (100 v/v)
C3
  • lithium acetate BCC 2 M -
NA
800.0 μl of lithium acetate BCC (2.5 M)
200.0 μl of water (100 v/v)
C4
  • lithium chloride BCC 2 M -
  • tergitol NP-40 BCC 0.01 w/v -
NA
200.0 μl of lithium chloride BCC (10 M)
10.0 μl of tergitol NP-40 BCC (1 w/v)
790.0 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 0.2 M -
  • lithium chloride BCC 2.5 M -
NA
57.14 μl of ammonium sulfate BCC (3.5 M)
250.0 μl of lithium chloride BCC (10 M)
692.86 μl of water (100 v/v)
C6
  • lithium formate BCC 1 M -
  • imidazole BCC 0.1 M pH 6.5
pH 6.5
83.11 μl of imidazole BCC pH 6 (1 M)
16.89 μl of imidazole BCC pH 8 (1 M)
285.71 μl of lithium formate BCC (3.5 M)
614.29 μl of water (100 v/v)
C7
  • magnesium acetate BCC 1.5 M -
  • trehalose BCC 0.12 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of magnesium acetate BCC (2.5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
120.0 μl of trehalose BCC (1 M)
180.0 μl of water (100 v/v)
C8
  • magnesium acetate BCC 1.5 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of lithium sulfate BCC (1 M)
600.0 μl of magnesium acetate BCC (2.5 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
100.0 μl of water (100 v/v)
C9
  • magnesium chloride BCC 0.5 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
200.0 μl of magnesium chloride BCC (2.5 M)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
700.0 μl of water (100 v/v)
C10
  • potassium chloride BCC 1 M -
  • magnesium chloride BCC 1 M -
NA
400.0 μl of magnesium chloride BCC (2.5 M)
333.33 μl of potassium chloride BCC (3 M)
266.67 μl of water (100 v/v)
C11
  • magnesium chloride BCC 2 M -
  • sodium bicine BCC 0.1 M pH 9
pH 9
800.0 μl of magnesium chloride BCC (2.5 M)
12.37 μl of sodium bicine BCC pH 7.4 (1 M)
87.63 μl of sodium bicine BCC pH 9.4 (1 M)
100.0 μl of water (100 v/v)
C12
  • magnesium formate BCC 0.2 M -
NA
200.0 μl of magnesium formate BCC (1 M)
800.0 μl of water (100 v/v)
D1
  • magnesium formate BCC 0.3 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
300.0 μl of magnesium formate BCC (1 M)
600.0 μl of water (100 v/v)
D2
  • magnesium formate BCC 0.3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of magnesium formate BCC (1 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
600.0 μl of water (100 v/v)
D3
  • magnesium formate BCC 0.5 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
500.0 μl of magnesium formate BCC (1 M)
400.0 μl of water (100 v/v)
D4
  • magnesium formate BCC 0.5 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
500.0 μl of magnesium formate BCC (1 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • potassium dihydrogen phosphate BCC 0.5 M -
  • trisodium citrate-citric acid BCC 0.05 M pH 5.6
pH 5.6
500.0 μl of potassium dihydrogen phosphate BCC (1 M)
40.71 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
9.29 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
450.0 μl of water (100 v/v)
D6
  • 1-octanol BCC 2.5 v/v -
  • potassium formate BCC 1 M -
NA
25.0 μl of 1-octanol BCC (100 v/v)
200.0 μl of potassium formate BCC (5 M)
775.0 μl of water (100 v/v)
D7
  • potassium sodium tartrate BCC 0.8 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
400.0 μl of potassium sodium tartrate BCC (2 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
D8
  • potassium sodium tartrate BCC 0.8 M -
  • polyethylene glycol monomethyl ether 5000 BCC 0.5 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
25.0 μl of polyethylene glycol monomethyl ether 5000 BCC (20 w/v)
400.0 μl of potassium sodium tartrate BCC (2 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
475.0 μl of water (100 v/v)
D9
  • potassium sodium tartrate BCC 1 M -
NA
500.0 μl of potassium sodium tartrate BCC (2 M)
500.0 μl of water (100 v/v)
D10
  • diammonium tartrate BCC 1 M -
  • potassium sodium tartrate BCC 1 M -
NA
500.0 μl of diammonium tartrate BCC (2 M)
500.0 μl of potassium sodium tartrate BCC (2 M)
D11
  • potassium sodium tartrate BCC 1 M -
  • lithium sulfate BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of potassium sodium tartrate BCC (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
200.0 μl of water (100 v/v)
D12
  • potassium sodium tartrate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
500.0 μl of potassium sodium tartrate BCC (2 M)
40.0 μl of sodium chloride BCC (5 M)
360.0 μl of water (100 v/v)
E1
  • potassium sodium tartrate BCC 1 M -
  • lithium sulfate BCC 0.2 M -
  • CHES BCC 0.1 M pH 9.5
pH 9.5
79.78 μl of CHES BCC pH 10.3 (0.8 M)
45.22 μl of CHES BCC pH 8.3 (0.8 M)
200.0 μl of lithium sulfate BCC (1 M)
500.0 μl of potassium sodium tartrate BCC (2 M)
175.0 μl of water (100 v/v)
E2
  • potassium sodium tartrate BCC 1.4 M -
  • trehalose BCC 0.03 M -
  • sodium HEPES BCC 0.05 M pH 7.5
pH 7.5
700.0 μl of potassium sodium tartrate BCC (2 M)
25.0 μl of sodium HEPES BCC pH 6.5 (1 M)
25.0 μl of sodium HEPES BCC pH 8.5 (1 M)
30.0 μl of trehalose BCC (1 M)
220.0 μl of water (100 v/v)
E3
  • sodium acetate BCC 1.4 M -
  • sodium cacodylate BCC 0.1 M pH 6.5
pH 6.5
466.67 μl of sodium acetate BCC (3 M)
34.88 μl of sodium cacodylate BCC pH 5.3 (1 M)
65.12 μl of sodium cacodylate BCC pH 7.3 (1 M)
433.33 μl of water (100 v/v)
E4
  • sodium acetate BCC 1.4 M -
  • trehalose BCC 0.12 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
466.67 μl of sodium acetate BCC (3 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
120.0 μl of trehalose BCC (1 M)
313.33 μl of water (100 v/v)
E5
  • sodium acetate BCC 2.8 M -
NA
933.33 μl of sodium acetate BCC (3 M)
66.67 μl of water (100 v/v)
E6
  • sodium chloride BCC 1 M -
  • lithium chloride BCC 1 M -
NA
100.0 μl of lithium chloride BCC (10 M)
200.0 μl of sodium chloride BCC (5 M)
700.0 μl of water (100 v/v)
E7
  • sodium chloride BCC 1.4 M -
  • sodium bicine BCC 0.1 M pH 9.4
pH 9.4
100.0 μl of sodium bicine BCC pH 9.4 (1 M)
280.0 μl of sodium chloride BCC (5 M)
620.0 μl of water (100 v/v)
E8
  • sodium chloride BCC 1.5 M -
  • ethanol BCC 10 v/v -
NA
100.0 μl of ethanol BCC (100 v/v)
300.0 μl of sodium chloride BCC (5 M)
600.0 μl of water (100 v/v)
E9
  • sodium chloride BCC 2 M -
  • polyethylene glycol 6000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 10 v/v -
NA
200.0 μl of polyethylene glycol 6000 BCC (50 w/v)
117.65 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
400.0 μl of sodium chloride BCC (5 M)
282.35 μl of water (100 v/v)
E10
  • sodium chloride BCC 2 M -
  • polyethylene glycol 6000 BCC 10 w/v -
NA
200.0 μl of polyethylene glycol 6000 BCC (50 w/v)
400.0 μl of sodium chloride BCC (5 M)
400.0 μl of water (100 v/v)
E11
  • polyethylene glycol monomethyl ether 750 BCC 10 w/v -
  • sodium chloride BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
125.0 μl of polyethylene glycol monomethyl ether 750 BCC (80 w/v)
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
400.0 μl of sodium chloride BCC (5 M)
375.0 μl of water (100 v/v)
E12
  • sodium chloride BCC 2 M -
  • potassium dihydrogen phosphate BCC 0.1 M -
  • sodium dihydrogen phosphate BCC 0.1 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of potassium dihydrogen phosphate BCC (1 M)
400.0 μl of sodium chloride BCC (5 M)
33.33 μl of sodium dihydrogen phosphate BCC (3 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
366.67 μl of water (100 v/v)
F1
  • sodium chloride BCC 2 M -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • trehalose BCC 0.12 M -
  • sodium MES BCC 0.1 M pH 6.5
pH 6.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
400.0 μl of sodium chloride BCC (5 M)
27.77 μl of sodium MES BCC pH 5.2 (1 M)
72.23 μl of sodium MES BCC pH 7.2 (1 M)
120.0 μl of trehalose BCC (1 M)
330.0 μl of water (100 v/v)
F2
  • sodium chloride BCC 2 M -
  • polyethylene glycol 400 BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of sodium chloride BCC (5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
375.0 μl of water (100 v/v)
F3
  • sodium chloride BCC 2 M -
  • polyethylene glycol 400 BCC 10 v/v -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of polyethylene glycol 400 BCC (80 v/v)
400.0 μl of sodium chloride BCC (5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
375.0 μl of water (100 v/v)
F4
  • sodium chloride BCC 2 M -
  • polyethylene glycol monomethyl ether 550 BCC 7 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
82.35 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
400.0 μl of sodium chloride BCC (5 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
417.65 μl of water (100 v/v)
F5
  • sodium chloride BCC 2.5 M -
  • sodium dihydrogen phosphate BCC 0.1 M -
NA
500.0 μl of sodium chloride BCC (5 M)
33.33 μl of sodium dihydrogen phosphate BCC (3 M)
466.67 μl of water (100 v/v)
F6
  • sodium chloride BCC 2.5 M -
  • lithium sulfate BCC 0.2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
200.0 μl of lithium sulfate BCC (1 M)
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
500.0 μl of sodium chloride BCC (5 M)
200.0 μl of water (100 v/v)
F7
  • sodium chloride BCC 2.5 M -
  • magnesium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
80.0 μl of magnesium chloride BCC (2.5 M)
500.0 μl of sodium chloride BCC (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
320.0 μl of water (100 v/v)
F8
  • sodium chloride BCC 2.5 M -
  • magnesium nitrate BCC 0.02 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
20.0 μl of magnesium nitrate BCC (1 M)
500.0 μl of sodium chloride BCC (5 M)
54.69 μl of tris chloride BCC pH 7 (1 M)
45.31 μl of tris chloride BCC pH 9 (1 M)
380.0 μl of water (100 v/v)
F9
  • sodium chloride BCC 2.5 M -
  • imidazole BCC 0.1 M pH 8
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
500.0 μl of sodium chloride BCC (5 M)
400.0 μl of water (100 v/v)
F10
  • sodium chloride BCC 3 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 3.5
pH 3.5
600.0 μl of sodium chloride BCC (5 M)
29.83 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
70.17 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
300.0 μl of water (100 v/v)
F11
  • sodium chloride BCC 3 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 4.5
pH 4.5
31.29 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
68.71 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600.0 μl of sodium chloride BCC (5 M)
300.0 μl of water (100 v/v)
F12
  • sodium chloride BCC 3 M -
  • n-octyl-b-D-Glucopyranoside BCC 0.5 w/v -
  • sodium acetate-acetic acid BCC 0.06 M pH 4.5
pH 4.5
166.67 μl of n-octyl-b-D-Glucopyranoside BCC (3 w/v)
18.77 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
41.23 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
600.0 μl of sodium chloride BCC (5 M)
173.33 μl of water (100 v/v)
G1
  • sodium chloride BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 5.5
pH 5.5
100.0 μl of bis-tris chloride BCC pH 5.5 (1 M)
600.0 μl of sodium chloride BCC (5 M)
300.0 μl of water (100 v/v)
G2
  • sodium chloride BCC 3 M -
  • bis-tris chloride BCC 0.1 M pH 6.5
pH 6.5
100.0 μl of bis-tris chloride BCC pH 6.5 (1 M)
600.0 μl of sodium chloride BCC (5 M)
300.0 μl of water (100 v/v)
G3
  • sodium chloride BCC 3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
600.0 μl of sodium chloride BCC (5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G4
  • sodium chloride BCC 3 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of sodium chloride BCC (5 M)
21.23 μl of tris chloride BCC pH 7 (1 M)
78.77 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
G5
  • sodium chloride BCC 3 M -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • trehalose BCC 0.12 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
600.0 μl of sodium chloride BCC (5 M)
120.0 μl of trehalose BCC (1 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
180.0 μl of water (100 v/v)
G6
  • sodium chloride BCC 4.3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
860.0 μl of sodium chloride BCC (5 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
40.0 μl of water (100 v/v)
G7
  • sodium dihydrogen phosphate BCC 0.4 M -
  • sodium chloride BCC 0.2 M -
  • imidazole BCC 0.1 M pH 8
pH 8
100.0 μl of imidazole BCC pH 8 (1 M)
40.0 μl of sodium chloride BCC (5 M)
133.33 μl of sodium dihydrogen phosphate BCC (3 M)
726.67 μl of water (100 v/v)
G8
  • sodium dihydrogen phosphate BCC 0.52 M -
  • dipotassium hydrogen phosphate BCC 0.28 M -
NA
93.33 μl of dipotassium hydrogen phosphate BCC (3 M)
173.33 μl of sodium dihydrogen phosphate BCC (3 M)
733.34 μl of water (100 v/v)
G9
  • sodium dihydrogen phosphate BCC 0.72 M -
  • dipotassium hydrogen phosphate BCC 0.08 M -
  • trisodium citrate-citric acid BCC 0.1 M pH 5.5
pH 5.5
26.67 μl of dipotassium hydrogen phosphate BCC (3 M)
240.0 μl of sodium dihydrogen phosphate BCC (3 M)
79.0 μl of trisodium citrate-citric acid BCC pH 8.1 (1 M)
21.0 μl of trisodium citrate-citric acid BCC pH 2.3 (1 M)
633.33 μl of water (100 v/v)
G10
  • sodium dihydrogen phosphate BCC 0.8 M -
  • ammonium dihydrogen phosphate BCC 0.8 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
320.0 μl of ammonium dihydrogen phosphate BCC (2.5 M)
266.67 μl of sodium dihydrogen phosphate BCC (3 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
313.33 μl of water (100 v/v)
G11
  • sodium dihydrogen phosphate BCC 0.91 M -
  • dipotassium hydrogen phosphate BCC 0.49 M -
NA
163.33 μl of dipotassium hydrogen phosphate BCC (3 M)
303.33 μl of sodium dihydrogen phosphate BCC (3 M)
533.34 μl of water (100 v/v)
G12
  • sodium dihydrogen phosphate BCC 0.98 M -
  • dipotassium hydrogen phosphate BCC 0.02 M -
NA
6.67 μl of dipotassium hydrogen phosphate BCC (3 M)
326.67 μl of sodium dihydrogen phosphate BCC (3 M)
666.66 μl of water (100 v/v)
H1
  • sodium dihydrogen phosphate BCC 1.17 M -
  • dipotassium hydrogen phosphate BCC 0.63 M -
NA
210.0 μl of dipotassium hydrogen phosphate BCC (3 M)
390.0 μl of sodium dihydrogen phosphate BCC (3 M)
400.0 μl of water (100 v/v)
H2
  • sodium dihydrogen phosphate BCC 1.2 M -
  • lithium sulfate BCC 0.2 M -
  • CAPS BCC 0.1 M pH 10.5
pH 10.5
85.99 μl of CAPS BCC pH 9.4 (0.5 M)
114.01 μl of CAPS BCC pH 11.4 (0.5 M)
200.0 μl of lithium sulfate BCC (1 M)
400.0 μl of sodium dihydrogen phosphate BCC (3 M)
200.0 μl of water (100 v/v)
H3
  • sodium dihydrogen phosphate BCC 1.37 M -
  • dipotassium hydrogen phosphate BCC 0.028 M -
NA
9.33 μl of dipotassium hydrogen phosphate BCC (3 M)
457.33 μl of sodium dihydrogen phosphate BCC (3 M)
533.34 μl of water (100 v/v)
H4
  • sodium dihydrogen phosphate BCC 1.6 M -
  • dipotassium hydrogen phosphate BCC 0.4 M -
  • phosphate-citrate BCC 0.1 M pH 4.2
pH 4.2
133.33 μl of dipotassium hydrogen phosphate BCC (3 M)
86.12 μl of phosphate-citrate BCC pH 2.2 (0.5 M)
113.88 μl of phosphate-citrate BCC pH 9.3 (1 M)
533.33 μl of sodium dihydrogen phosphate BCC (3 M)
133.34 μl of water (100 v/v)
H5
  • sodium formate BCC 2 M -
  • sodium acetate-acetic acid BCC 0.1 M pH 5
pH 5
65.55 μl of sodium acetate-acetic acid BCC pH 5.8 (1 M)
34.45 μl of sodium acetate-acetic acid BCC pH 3.8 (1 M)
285.71 μl of sodium formate BCC (7 M)
614.29 μl of water (100 v/v)
H6
  • sodium formate BCC 2 M -
  • ADA BCC 0.05 M pH 6.5
pH 6.5
54.69 μl of ADA BCC pH 5.5 (0.5 M)
45.31 μl of ADA BCC pH 7.5 (0.5 M)
285.71 μl of sodium formate BCC (7 M)
614.29 μl of water (100 v/v)
H7
  • sodium formate BCC 3 M -
  • sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
428.57 μl of sodium formate BCC (7 M)
50.0 μl of sodium HEPES BCC pH 6.5 (1 M)
50.0 μl of sodium HEPES BCC pH 8.5 (1 M)
471.43 μl of water (100 v/v)
H8
  • sodium formate BCC 3.5 M -
  • zinc sulfate BCC 0.01 M -
NA
500.0 μl of sodium formate BCC (7 M)
100.0 μl of zinc sulfate BCC (0.1 M)
400.0 μl of water (100 v/v)
H9
  • sodium formate BCC 4 M -
NA
571.43 μl of sodium formate BCC (7 M)
428.57 μl of water (100 v/v)
H10
  • sodium formate BCC 4 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
571.43 μl of sodium formate BCC (7 M)
10.62 μl of tris chloride BCC pH 7 (1 M)
39.38 μl of tris chloride BCC pH 9 (1 M)
378.57 μl of water (100 v/v)
H11
  • trichloroacetic acid BCC 40 w/v -
  • sodium chloride BCC 2 M -
NA
400.0 μl of sodium chloride BCC (5 M)
400.0 μl of trichloroacetic acid BCC (100 w/v)
200.0 μl of water (100 v/v)
H12
  • trisodium citrate BCC 1 M -
  • sodium chloride BCC 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
40.0 μl of sodium chloride BCC (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
625.0 μl of trisodium citrate BCC (1.6 M)
235.0 μl of water (100 v/v)