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Supplemental Information

Screen Description

BCC Core Screens / Morpheus_C3
Created 2025-10-09.
Based on Molecular Dimensions Morpheus screen, made up by C3. Mixtures are delineated into their individual components.

Rigaku Recipe XML

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Barcodes

  • Z0002721 Lot 4 (1500.0 µl)
  • ZT928f Lot 6 (1500.0 µl)
  • ZT928g Lot 7 (1500.0 µl)

Screen Structure

Well Count 96
Number of distinct ingredients 46
Internal diversity (score mean dist) 0.880

Screen Composition

2025-10-21T23:05:12.611799 image/svg+xml Matplotlib v3.10.6, https://matplotlib.org/
ingredient type Morpheus_C3
Buffer 132
Cryoprotectant 36
Organic (non-volatile) 216
Organic (volatile) 60
Polymer 144
Salt 180

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Morpheus_C3 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
204.7 μl of water (100 v/v)
A2
  • calcium chloride BCC 0.03 M -
  • ethylene glycol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
30.0 μl of calcium chloride BCC (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
30.0 μl of magnesium chloride BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
140.0 μl of water (100 v/v)
A3
  • calcium chloride BCC 0.03 M -
  • glycerol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
30.0 μl of calcium chloride BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
30.0 μl of magnesium chloride BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
390.0 μl of water (100 v/v)
A4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
215.0 μl of water (100 v/v)
A5
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
204.7 μl of water (100 v/v)
A6
  • calcium chloride BCC 0.03 M -
  • ethylene glycol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
30.0 μl of calcium chloride BCC (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
30.0 μl of magnesium chloride BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
140.0 μl of water (100 v/v)
A7
  • calcium chloride BCC 0.03 M -
  • glycerol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
30.0 μl of calcium chloride BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
30.0 μl of magnesium chloride BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
390.0 μl of water (100 v/v)
A8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
215.0 μl of water (100 v/v)
A9
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
204.7 μl of water (100 v/v)
A10
  • calcium chloride BCC 0.03 M -
  • ethylene glycol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
30.0 μl of calcium chloride BCC (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
30.0 μl of magnesium chloride BCC (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
140.0 μl of water (100 v/v)
A11
  • calcium chloride BCC 0.03 M -
  • glycerol BCC 20 v/v -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 4000 BCC 10 w/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
30.0 μl of calcium chloride BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
30.0 μl of magnesium chloride BCC (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
390.0 μl of water (100 v/v)
A12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • calcium chloride BCC 0.03 M -
  • magnesium chloride BCC 0.03 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
30.0 μl of calcium chloride BCC (1 M)
30.0 μl of magnesium chloride BCC (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
215.0 μl of water (100 v/v)
B1
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
174.7 μl of water (100 v/v)
B2
  • ethylene glycol BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of ethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
110.0 μl of water (100 v/v)
B3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
250.0 μl of glycerol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
360.0 μl of water (100 v/v)
B4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
185.0 μl of water (100 v/v)
B5
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
174.7 μl of water (100 v/v)
B6
  • ethylene glycol BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of ethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
110.0 μl of water (100 v/v)
B7
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
250.0 μl of glycerol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
360.0 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
185.0 μl of water (100 v/v)
B9
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
174.7 μl of water (100 v/v)
B10
  • ethylene glycol BCC 20 v/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
110.0 μl of water (100 v/v)
B11
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of glycerol BCC (80 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
360.0 μl of water (100 v/v)
B12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium fluoride BCC 0.03 M -
  • sodium iodide BCC 0.03 M -
  • sodium bromide BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium bromide BCC (1 M)
30.0 μl of sodium fluoride BCC (1 M)
30.0 μl of sodium iodide BCC (1 M)
185.0 μl of water (100 v/v)
C1
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium nitrate BCC (1 M)
144.7 μl of water (100 v/v)
C2
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • ethylene glycol BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MES-imidazole BCC 0.1 M pH 6.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium nitrate BCC (1 M)
80.0 μl of water (100 v/v)
C3
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium nitrate BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
250.0 μl of glycerol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
330.0 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium nitrate BCC 0.03 M -
  • MES-imidazole BCC 0.1 M pH 6.5
NA
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
155.0 μl of water (100 v/v)
C5
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium nitrate BCC (1 M)
144.7 μl of water (100 v/v)
C6
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • ethylene glycol BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium nitrate BCC (1 M)
80.0 μl of water (100 v/v)
C7
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium nitrate BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
250.0 μl of glycerol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
330.0 μl of water (100 v/v)
C8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium nitrate BCC 0.03 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
NA
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of ammonium sulfate BCC (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
155.0 μl of water (100 v/v)
C9
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
30.0 μl of sodium nitrate BCC (1 M)
144.7 μl of water (100 v/v)
C10
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • ethylene glycol BCC 20 v/v -
  • sodium nitrate BCC 0.03 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • bicine-tris BCC 0.1 M pH 8.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
30.0 μl of sodium nitrate BCC (1 M)
80.0 μl of water (100 v/v)
C11
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • sodium nitrate BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
NA
30.0 μl of ammonium sulfate BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
250.0 μl of glycerol BCC (80 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
330.0 μl of water (100 v/v)
C12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium sulfate BCC 0.03 M -
  • disodium hydrogen phosphate BCC 0.03 M pH 9.3
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • sodium nitrate BCC 0.03 M -
  • bicine-tris BCC 0.1 M pH 8.5
NA
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
30.0 μl of ammonium sulfate BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
60.0 μl of disodium hydrogen phosphate BCC pH 9.3 (0.5 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
30.0 μl of sodium nitrate BCC (1 M)
155.0 μl of water (100 v/v)
D1
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
176.4 μl of water (100 v/v)
D2
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • ethylene glycol BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • 1-butanol BCC 1.83 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
200.0 μl of ethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
111.7 μl of water (100 v/v)
D3
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
250.0 μl of glycerol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
361.7 μl of water (100 v/v)
D4
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
186.7 μl of water (100 v/v)
D5
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
176.4 μl of water (100 v/v)
D6
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • ethylene glycol BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • 1-butanol BCC 1.83 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
200.0 μl of ethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
111.7 μl of water (100 v/v)
D7
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
250.0 μl of glycerol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
361.7 μl of water (100 v/v)
D8
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
186.7 μl of water (100 v/v)
D9
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
176.4 μl of water (100 v/v)
D10
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • ethylene glycol BCC 20 v/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • 1-butanol BCC 1.83 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
111.7 μl of water (100 v/v)
D11
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-propanol BCC 1.53 v/v -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of glycerol BCC (80 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
361.7 μl of water (100 v/v)
D12
  • 1,2-propanediol BCC 1.47 v/v -
  • 1,4-butanediol BCC 1.77 v/v -
  • 1,6-hexanediol BCC 0.02 M -
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • 2-propanol BCC 1.53 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • 1,3-propanediol BCC 1.45 v/v -
  • 1-butanol BCC 1.83 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
14.7 μl of 1,2-propanediol BCC (100 v/v)
14.5 μl of 1,3-propanediol BCC (100 v/v)
17.7 μl of 1,4-butanediol BCC (100 v/v)
4.0 μl of 1,6-hexanediol BCC (5 M)
18.3 μl of 1-butanol BCC (100 v/v)
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
19.1 μl of 2-propanol BCC (80 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
186.7 μl of water (100 v/v)
E1
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
28.5 μl of diethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
79.7 μl of water (100 v/v)
E2
  • ethylene glycol BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
28.5 μl of diethylene glycol BCC (100 v/v)
200.0 μl of ethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
15.0 μl of water (100 v/v)
E3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
28.5 μl of diethylene glycol BCC (100 v/v)
250.0 μl of glycerol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
265.0 μl of water (100 v/v)
E4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.5 μl of diethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
90.0 μl of water (100 v/v)
E5
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
28.5 μl of diethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
79.7 μl of water (100 v/v)
E6
  • ethylene glycol BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
28.5 μl of diethylene glycol BCC (100 v/v)
200.0 μl of ethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
15.0 μl of water (100 v/v)
E7
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
28.5 μl of diethylene glycol BCC (100 v/v)
250.0 μl of glycerol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
265.0 μl of water (100 v/v)
E8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.5 μl of diethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
63.5 μl of pentaethylene glycol BCC (100 v/v)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
90.0 μl of water (100 v/v)
E9
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
28.5 μl of diethylene glycol BCC (100 v/v)
63.5 μl of pentaethylene glycol BCC (100 v/v)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
79.7 μl of water (100 v/v)
E10
  • ethylene glycol BCC 20 v/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • polyethylene glycol 8000 BCC 10 w/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
28.5 μl of diethylene glycol BCC (100 v/v)
200.0 μl of ethylene glycol BCC (100 v/v)
63.5 μl of pentaethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
15.0 μl of water (100 v/v)
E11
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
28.5 μl of diethylene glycol BCC (100 v/v)
250.0 μl of glycerol BCC (80 v/v)
63.5 μl of pentaethylene glycol BCC (100 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
265.0 μl of water (100 v/v)
E12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • triethylene glycol BCC 4.1 v/v -
  • diethylene glycol BCC 2.85 v/v -
  • tetraethylene glycol BCC 5.2 v/v -
  • pentaethylene glycol BCC 6.35 v/v -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
28.5 μl of diethylene glycol BCC (100 v/v)
63.5 μl of pentaethylene glycol BCC (100 v/v)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
52.0 μl of tetraethylene glycol BCC (100 v/v)
41.0 μl of triethylene glycol BCC (100 v/v)
90.0 μl of water (100 v/v)
F1
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.0 μl of xylose BCC (1 M)
144.7 μl of water (100 v/v)
F2
  • ethylene glycol BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
20.0 μl of xylose BCC (1 M)
80.0 μl of water (100 v/v)
F3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
330.0 μl of water (100 v/v)
F4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
155.0 μl of water (100 v/v)
F5
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.0 μl of xylose BCC (1 M)
144.7 μl of water (100 v/v)
F6
  • ethylene glycol BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
20.0 μl of xylose BCC (1 M)
80.0 μl of water (100 v/v)
F7
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
330.0 μl of water (100 v/v)
F8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
155.0 μl of water (100 v/v)
F9
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
20.0 μl of xylose BCC (1 M)
144.7 μl of water (100 v/v)
F10
  • ethylene glycol BCC 20 v/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
20.0 μl of xylose BCC (1 M)
80.0 μl of water (100 v/v)
F11
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
330.0 μl of water (100 v/v)
F12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glucose BCC 0.02 M -
  • galactose BCC 0.02 M -
  • mannose BCC 0.02 M -
  • L-fucose BCC 0.02 M -
  • xylose BCC 0.02 M -
  • N-acetylglucosamine BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
20.0 μl of galactose BCC (1 M)
20.0 μl of glucose BCC (1 M)
20.0 μl of L-fucose BCC (1 M)
20.0 μl of mannose BCC (1 M)
20.0 μl of N-acetylglucosamine BCC (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
20.0 μl of xylose BCC (1 M)
155.0 μl of water (100 v/v)
G1
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
4.0 μl of ammonium acetate BCC (5 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
133.2 μl of water (100 v/v)
G2
  • ammonium acetate BCC 0.02 M -
  • ethylene glycol BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • sodium oxamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
4.0 μl of ammonium acetate BCC (5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
68.5 μl of water (100 v/v)
G3
  • ammonium acetate BCC 0.02 M -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
4.0 μl of ammonium acetate BCC (5 M)
250.0 μl of glycerol BCC (80 v/v)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
318.5 μl of water (100 v/v)
G4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of ammonium acetate BCC (5 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
143.5 μl of water (100 v/v)
G5
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
4.0 μl of ammonium acetate BCC (5 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
133.2 μl of water (100 v/v)
G6
  • ammonium acetate BCC 0.02 M -
  • ethylene glycol BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • sodium oxamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
4.0 μl of ammonium acetate BCC (5 M)
200.0 μl of ethylene glycol BCC (100 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
68.5 μl of water (100 v/v)
G7
  • ammonium acetate BCC 0.02 M -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
4.0 μl of ammonium acetate BCC (5 M)
250.0 μl of glycerol BCC (80 v/v)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
318.5 μl of water (100 v/v)
G8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of ammonium acetate BCC (5 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
143.5 μl of water (100 v/v)
G9
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
4.0 μl of ammonium acetate BCC (5 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
133.2 μl of water (100 v/v)
G10
  • ammonium acetate BCC 0.02 M -
  • ethylene glycol BCC 20 v/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • sodium oxamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
4.0 μl of ammonium acetate BCC (5 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
68.5 μl of water (100 v/v)
G11
  • ammonium acetate BCC 0.02 M -
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
4.0 μl of ammonium acetate BCC (5 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of glycerol BCC (80 v/v)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
318.5 μl of water (100 v/v)
G12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • ammonium acetate BCC 0.02 M -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • potassium sodium tartrate BCC 0.02 M -
  • sodium formate BCC 0.02 M -
  • trisodium citrate BCC 0.02 M -
  • sodium oxamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
4.0 μl of ammonium acetate BCC (5 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
10.0 μl of potassium sodium tartrate BCC (2 M)
5.0 μl of sodium formate BCC (4 M)
100.0 μl of sodium oxamate BCC (0.2 M)
12.5 μl of trisodium citrate BCC (1.6 M)
143.5 μl of water (100 v/v)
H1
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of alanine BCC (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
84.7 μl of water (100 v/v)
H2
  • ethylene glycol BCC 20 v/v -
  • glycine BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of alanine BCC (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
20.0 μl of water (100 v/v)
H3
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
20.0 μl of alanine BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
270.0 μl of water (100 v/v)
H4
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MES-imidazole BCC 0.1 M pH 6.5
pH 6.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of alanine BCC (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
44.0 μl of MES-imidazole BCC pH 9 (1 M)
56.0 μl of MES-imidazole BCC pH 3.8 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
95.0 μl of water (100 v/v)
H5
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of alanine BCC (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
84.7 μl of water (100 v/v)
H6
  • ethylene glycol BCC 20 v/v -
  • glycine BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of alanine BCC (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
20.0 μl of water (100 v/v)
H7
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
20.0 μl of alanine BCC (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
270.0 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • MOPS-sodium HEPES BCC 0.1 M pH 7.5
pH 7.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of alanine BCC (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
57.6 μl of MOPS-sodium HEPES BCC pH 9.1 (1 M)
42.4 μl of MOPS-sodium HEPES BCC pH 4.7 (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
95.0 μl of water (100 v/v)
H9
  • polyethylene glycol 20000 BCC 10 w/v -
  • polyethylene glycol monomethyl ether 550 BCC 20 v/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
20.0 μl of alanine BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
400.0 μl of polyethylene glycol 20000 BCC (25 w/v)
235.3 μl of polyethylene glycol monomethyl ether 550 BCC (85 v/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
84.7 μl of water (100 v/v)
H10
  • ethylene glycol BCC 20 v/v -
  • glycine BCC 0.02 M -
  • polyethylene glycol 8000 BCC 10 w/v -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
20.0 μl of alanine BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
200.0 μl of ethylene glycol BCC (100 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
500.0 μl of polyethylene glycol 8000 BCC (20 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
20.0 μl of water (100 v/v)
H11
  • glycerol BCC 20 v/v -
  • polyethylene glycol 4000 BCC 10 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
20.0 μl of alanine BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
250.0 μl of glycerol BCC (80 v/v)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
200.0 μl of polyethylene glycol 4000 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
270.0 μl of water (100 v/v)
H12
  • 2-methyl-2,4-pentanediol BCC 12.5 v/v -
  • polyethylene glycol 1000 BCC 12.5 w/v -
  • polyethylene glycol 3350 BCC 12.5 w/v -
  • glycine BCC 0.02 M -
  • lysine BCC 0.02 M -
  • alanine BCC 0.02 M -
  • serine BCC 0.02 M -
  • sodium glutamate BCC 0.02 M -
  • bicine-tris BCC 0.1 M pH 8.5
pH 8.5
125.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of alanine BCC (1 M)
61.4 μl of bicine-tris BCC pH 10.1 (1 M)
38.6 μl of bicine-tris BCC pH 5.1 (1 M)
20.0 μl of glycine BCC (1 M)
20.0 μl of lysine BCC (1 M)
250.0 μl of polyethylene glycol 1000 BCC (50 w/v)
250.0 μl of polyethylene glycol 3350 BCC (50 w/v)
100.0 μl of serine BCC (0.2 M)
20.0 μl of sodium glutamate BCC (1 M)
95.0 μl of water (100 v/v)