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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
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Supplemental Information
Screen Description
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Structure II
Barcodes
Structure II
Screen Structure
Structure II
Well Count | 96 |
---|---|
Number of distinct ingredients | 48 |
Internal diversity (score mean dist) | 0.954 |
Screen Composition
ingredient type | Structure II |
---|---|
Buffer | 46 |
Cryoprotectant | 1 |
Detergent | 1 |
Organic (non-volatile) | 17 |
Organic (volatile) | 6 |
Polymer | 19 |
Salt | 54 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 20 μl of Sodium chloride (5 M) 600 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 9 μl of BICINE pH 7.4 (1 M) 280 μl of water (100 v/v) |
A2 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 9 μl of BICINE pH 7.4 (1 M) 500 μl of Magnesium chloride (4 M) 400 μl of water (100 v/v) |
A3 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 20 μl of 1,4-Dioxane (100 v/v) 9 μl of BICINE pH 7.4 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 546 μl of water (100 v/v) |
A4 |
| pH 8.5 | 566 μl of 1,6-Hexanediol (6 M) 100 μl of Magnesium chloride (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 233 μl of water (100 v/v) |
A5 |
| pH 8.5 | 250 μl of 2-Methyl-2-propanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 650 μl of water (100 v/v) |
A6 |
| pH 8.5 | 2 μl of Nickel (II) chloride (4 M) 500 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 397 μl of water (100 v/v) |
A7 |
| pH 8.5 | 428 μl of Ammonium sulfate (3.5 M) 120 μl of Glycerol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 351 μl of water (100 v/v) |
A8 |
| pH 8.5 | 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 80 μl of Ammonium di-hydrogen phosphate (2.5 M) 320 μl of water (100 v/v) |
A9 |
| pH 8.5 | 200 μl of Ethanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 700 μl of water (100 v/v) |
A10 |
| pH 8.5 | 2 μl of Nickel (II) chloride (4 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 497 μl of water (100 v/v) |
A11 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 142 μl of Ammonium sulfate (3.5 M) 48 μl of HEPES pH 8.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 457 μl of water (100 v/v) |
A12 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 650 μl of water (100 v/v) |
B1 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 200 μl of Jeffamine® M-600 (100 v/v) 700 μl of water (100 v/v) |
B2 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 457 μl of Ammonium sulfate (3.5 M) 48 μl of HEPES pH 8.5 (1 M) 20 μl of Sodium chloride (5 M) 422 μl of water (100 v/v) |
B3 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 200 μl of Ammonium formate (10 M) 700 μl of water (100 v/v) |
B4 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 333 μl of Sodium acetate (3 M) 48 μl of HEPES pH 8.5 (1 M) 50 μl of Cadmium sulfate (1 M) 516 μl of water (100 v/v) |
B5 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of water (100 v/v) |
B6 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 860 μl of Sodium chloride (5 M) 40 μl of water (100 v/v) |
B7 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 48 μl of HEPES pH 8.5 (1 M) 80 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 620 μl of water (100 v/v) |
B8 |
| pH 6.5 | 640 μl of Magnesium sulfate (2.5 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 160 μl of water (100 v/v) |
B9 |
| pH 6.5 | 48 μl of MES pH 5.2 (0.5 M) 50 μl of Potassium di-hydrogen phosphate (2 M) 400 μl of Sodium chloride (5 M) 200 μl of di-Sodium hydrogen phosphate (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 150 μl of water (100 v/v) |
B10 |
| pH 6.5 | 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 400 μl of Polyethylene glycol 20,000 (30 w/v) 400 μl of water (100 v/v) |
B11 |
| pH 6.5 | 100 μl of 1,4-Dioxane (100 v/v) 457 μl of Ammonium sulfate (3.5 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 242 μl of water (100 v/v) |
B12 |
| pH 6.5 | 50 μl of Cesium chloride (1 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 300 μl of Jeffamine® M-600 (100 v/v) 450 μl of water (100 v/v) |
C1 |
| pH 6.5 | 10 μl of Cobalt (II) chloride (1 M) 514 μl of Ammonium sulfate (3.5 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 275 μl of water (100 v/v) |
C2 |
| pH 6.5 | 57 μl of Ammonium sulfate (3.5 M) 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 142 μl of water (100 v/v) |
C3 |
| pH 6.5 | 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 500 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 5 μl of Zinc sulfate (2 M) 295 μl of water (100 v/v) |
C4 |
| pH 7.5 | 51 μl of HEPES pH 6.6 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 48 μl of HEPES pH 8.5 (1 M) 500 μl of water (100 v/v) |
C5 |
| pH 5.6 | 571 μl of Ammonium sulfate (3.5 M) 133 μl of Potassium Sodium tartrate (1.5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 232 μl of water (100 v/v) |
C6 |
| pH 5.6 | 142 μl of Ammonium sulfate (3.5 M) 500 μl of Lithium sulfate (2 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 294 μl of water (100 v/v) |
C7 |
| pH 5.6 | 80 μl of Ethylene imine polymer (50 v/v) 100 μl of Sodium chloride (5 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 757 μl of water (100 v/v) |
C8 |
| pH 5.6 | 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 350 μl of 2-Methyl-2-propanol (100 v/v) 587 μl of water (100 v/v) |
C9 |
| pH 5.6 | 10 μl of Iron (III) chloride (1 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 100 μl of Jeffamine® M-600 (100 v/v) 827 μl of water (100 v/v) |
C10 |
| pH 5.6 | 416 μl of 1,6-Hexanediol (6 M) 10 μl of Manganese (II) chloride (1 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 59 μl of tri-Sodium citrate pH 6.5 (1.6 M) 510 μl of water (100 v/v) |
C11 |
| pH 4.6 | 400 μl of Sodium chloride (5 M) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 500 μl of water (100 v/v) |
C12 |
| pH 4.6 | 40 μl of Sodium chloride (5 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 560 μl of water (100 v/v) |
D1 |
| pH 4.6 | 10 μl of Cobalt (II) chloride (1 M) 166 μl of 1,6-Hexanediol (6 M) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 723 μl of water (100 v/v) |
D2 |
| pH 4.6 | 300 μl of Polyethylene glycol 400 (100 v/v) 64 μl of Sodium acetate pH 4 (1 M) 100 μl of Cadmium chloride (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 500 μl of water (100 v/v) |
D3 |
| pH 4.6 | 57 μl of Ammonium sulfate (3.5 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 64 μl of Sodium acetate pH 4 (1 M) 35 μl of Sodium acetate pH 5.5 (1 M) 242 μl of water (100 v/v) |
D4 |
| NA | 200 μl of Polyethylene glycol 6,000 (50 w/v) 400 μl of Sodium chloride (5 M) 400 μl of water (100 v/v) |
D5 |
| NA | 100 μl of Sodium chloride (5 M) 50 μl of Magnesium chloride (2 M) 0 μl of Cetyltrimethylammonium bromide (50 mM) 849 μl of water (100 v/v) |
D6 |
| NA | 250 μl of Ethylene glycol (100 v/v) 750 μl of water (100 v/v) |
D7 |
| NA | 350 μl of 1,4-Dioxane (100 v/v) 650 μl of water (100 v/v) |
D8 |
| NA | 571 μl of Ammonium sulfate (3.5 M) 50 μl of 2-Propanol (100 v/v) 378 μl of water (100 v/v) |
D9 |
| pH 7 | 470 μl of Imidazole pH 8 (1 M) 529 μl of Imidazole pH 6.2 (1 M) |
D10 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v) |
D11 |
| NA | 300 μl of Sodium chloride (5 M) 100 μl of Ethanol (100 v/v) 600 μl of water (100 v/v) |
D12 |
| pH 6.5 | 8 μl of tri-Sodium citrate pH 3 (1.6 M) 992 μl of tri-Sodium citrate pH 6.5 (1.6 M) |
E1 |
| NA | 300 μl of Polyvinylpyrrolidone K15 (50 w/v) 700 μl of water (100 v/v) |
E2 |
| NA | 1000 μl of Urea (2 M) |
E3 | NA | ||
E4 | NA | ||
E5 | NA | ||
E6 | NA | ||
E7 | NA | ||
E8 | NA | ||
E9 | NA | ||
E10 | NA | ||
E11 | NA | ||
E12 | NA | ||
F1 | NA | ||
F2 | NA | ||
F3 | NA | ||
F4 | NA | ||
F5 | NA | ||
F6 | NA | ||
F7 | NA | ||
F8 | NA | ||
F9 | NA | ||
F10 | NA | ||
F11 | NA | ||
F12 | NA | ||
G1 | NA | ||
G2 | NA | ||
G3 | NA | ||
G4 | NA | ||
G5 | NA | ||
G6 | NA | ||
G7 | NA | ||
G8 | NA | ||
G9 | NA | ||
G10 | NA | ||
G11 | NA | ||
G12 | NA | ||
H1 | NA | ||
H2 | NA | ||
H3 | NA | ||
H4 | NA | ||
H5 | NA | ||
H6 | NA | ||
H7 | NA | ||
H8 | NA | ||
H9 | NA | ||
H10 | NA | ||
H11 | NA | ||
H12 | NA |