C6 - Structure II - Screen Contents

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Supplemental Information

Screen Description

Molecular Dimensions / Structure II

Created 2012-08-13.

Rigaku Recipe XML

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Screen Structure

Well Count	96
Number of distinct ingredients	48
Internal diversity (score mean dist)	0.96

Screen Composition

More information about screen composition chart.

ingredient type	Structure II
Buffer	46
Cryoprotectant	1
Detergent	1
Organic (non-volatile)	17
Organic (volatile)	6
Polymer	19
Salt	54

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Structure II Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 0.1 M - BICINE 0.1 M pH 9 Polyethylene glycol monomethyl ether 550 30 w/v - 🔍	pH 9	9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 20.0 μl of Sodium chloride (5 M) 280.0 μl of water (100 v/v)
A2	BICINE 0.1 M pH 9 Magnesium chloride 2 M - 🔍	pH 9	9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 500.0 μl of Magnesium chloride (4 M) 400.0 μl of water (100 v/v)
A3	1,4-Dioxane 2 v/v - BICINE 0.1 M pH 9 Polyethylene glycol 20,000 10 w/v - 🔍	pH 9	20.0 μl of 1,4-Dioxane (100 v/v) 9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 546.7 μl of water (100 v/v)
A4	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 1,6-Hexanediol 3.4 M - 🔍	pH 8.5	566.7 μl of 1,6-Hexanediol (6 M) 100.0 μl of Magnesium chloride (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v)
A5	tris chloride BCC 0.1 M pH 8.5 2-Methyl-2-propanol 25 v/v - 🔍	pH 8.5	250.0 μl of 2-Methyl-2-propanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 650.0 μl of water (100 v/v)
A6	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 1 M - 🔍	pH 8.5	500.0 μl of Lithium sulfate (2 M) 2.5 μl of Nickel (II) chloride (4 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 397.5 μl of water (100 v/v)
A7	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Glycerol 12 v/v - 🔍	pH 8.5	428.6 μl of Ammonium sulfate (3.5 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 351.4 μl of water (100 v/v)
A8	Ammonium di-hydrogen phosphate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
A9	tris chloride BCC 0.1 M pH 8.5 Ethanol 20 v/v - 🔍	pH 8.5	200.0 μl of Ethanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v)
A10	Nickel (II) chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol monomethyl ether 2,000 20 w/v - 🔍	pH 8.5	2.5 μl of Nickel (II) chloride (4 M) 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 497.5 μl of water (100 v/v)
A11	Ammonium sulfate 0.5 M - HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 7.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 142.9 μl of Ammonium sulfate (3.5 M) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 457.1 μl of water (100 v/v)
A12	HEPES 0.1 M pH 7.5 Polyethylene glycol 6,000 10 w/v - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 7.5	50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 650.0 μl of water (100 v/v)
B1	HEPES 0.1 M pH 7.5 Jeffamine® M-600 20 v/v - 🔍	pH 7.5	51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 200.0 μl of Jeffamine® M-600 (100 v/v) 700.0 μl of water (100 v/v)
B2	Sodium chloride 0.1 M - HEPES 0.1 M pH 7.5 Ammonium sulfate 1.6 M - 🔍	pH 7.5	457.1 μl of Ammonium sulfate (3.5 M) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 20.0 μl of Sodium chloride (5 M) 422.9 μl of water (100 v/v)
B3	HEPES 0.1 M pH 7.5 Ammonium formate 2 M - 🔍	pH 7.5	200.0 μl of Ammonium formate (10 M) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 700.0 μl of water (100 v/v)
B4	Cadmium sulfate 0.05 M - HEPES 0.1 M pH 7.5 Sodium acetate 1 M - 🔍	pH 7.5	50.0 μl of Cadmium sulfate (1 M) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 333.3 μl of Sodium acetate (3 M) 516.7 μl of water (100 v/v)
B5	HEPES 0.1 M pH 7.5 2-methyl-2,4-pentanediol BCC 70 v/v - 🔍	pH 7.5	700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 200.0 μl of water (100 v/v)
B6	HEPES 0.1 M pH 7.5 Sodium chloride 4.3 M - 🔍	pH 7.5	51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 860.0 μl of Sodium chloride (5 M) 40.0 μl of water (100 v/v)
B7	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 10 w/v - Ethylene glycol 8 v/v - 🔍	pH 7.5	80.0 μl of Ethylene glycol (100 v/v) 51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v)
B8	MES 0.1 M pH 6.5 Magnesium sulfate 1.6 M - 🔍	pH 6.5	640.0 μl of Magnesium sulfate (2.5 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 160.0 μl of water (100 v/v)
B9	di-Sodium hydrogen phosphate 0.1 M - Potassium di-hydrogen phosphate 0.1 M - MES 0.1 M pH 6.5 Sodium chloride 2 M - 🔍	pH 6.5	200.0 μl of di-Sodium hydrogen phosphate (0.5 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 50.0 μl of Potassium di-hydrogen phosphate (2 M) 400.0 μl of Sodium chloride (5 M) 150.0 μl of water (100 v/v)
B10	MES 0.1 M pH 6.5 Polyethylene glycol 20,000 12 w/v - 🔍	pH 6.5	48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 400.0 μl of Polyethylene glycol 20,000 (30 w/v) 400.0 μl of water (100 v/v)
B11	Ammonium sulfate 1.6 M - MES 0.1 M pH 6.5 1,4-Dioxane 10 v/v - 🔍	pH 6.5	100.0 μl of 1,4-Dioxane (100 v/v) 457.1 μl of Ammonium sulfate (3.5 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 242.9 μl of water (100 v/v)
B12	Cesium chloride 0.05 M - MES 0.1 M pH 6.5 Jeffamine® M-600 30 v/v - 🔍	pH 6.5	50.0 μl of Cesium chloride (1 M) 300.0 μl of Jeffamine® M-600 (100 v/v) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 450.0 μl of water (100 v/v)
C1	Cobalt (II) chloride 0.01 M - MES 0.1 M pH 6.5 Ammonium sulfate 1.8 M - 🔍	pH 6.5	514.3 μl of Ammonium sulfate (3.5 M) 10.0 μl of Cobalt (II) chloride (1 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 275.7 μl of water (100 v/v)
C2	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	57.1 μl of Ammonium sulfate (3.5 M) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 142.9 μl of water (100 v/v)
C3	Zinc sulfate 0.01 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 25 w/v - 🔍	pH 6.5	48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 500.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v) 5.0 μl of Zinc sulfate (2 M) 295.0 μl of water (100 v/v)
C4	HEPES 0.1 M pH 7.5 Polyethylene glycol 10,000 20 w/v - 🔍	pH 7.5	51.3 μl of HEPES pH 6.6 (1 M) 48.7 μl of HEPES pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v)
C5	Potassium Sodium tartrate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Ammonium sulfate 2 M - 🔍	pH 5.6	571.4 μl of Ammonium sulfate (3.5 M) 133.3 μl of Potassium Sodium tartrate (1.5 M) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 232.7 μl of water (100 v/v)
C6	Ammonium sulfate 0.5 M - tri-Sodium citrate 0.1 M pH 5.6 Lithium sulfate 1 M - 🔍	pH 5.6	142.9 μl of Ammonium sulfate (3.5 M) 500.0 μl of Lithium sulfate (2 M) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 294.6 μl of water (100 v/v)
C7	Sodium chloride 0.5 M - tri-Sodium citrate 0.1 M pH 5.6 Ethylene imine polymer 4 v/v - 🔍	pH 5.6	80.0 μl of Ethylene imine polymer (50 v/v) 100.0 μl of Sodium chloride (5 M) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 757.5 μl of water (100 v/v)
C8	tri-Sodium citrate 0.1 M pH 5.6 2-Methyl-2-propanol 35 v/v - 🔍	pH 5.6	350.0 μl of 2-Methyl-2-propanol (100 v/v) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 587.5 μl of water (100 v/v)
C9	Iron (III) chloride 0.01 M - tri-Sodium citrate 0.1 M pH 5.6 Jeffamine® M-600 10 v/v - 🔍	pH 5.6	10.0 μl of Iron (III) chloride (1 M) 100.0 μl of Jeffamine® M-600 (100 v/v) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 827.5 μl of water (100 v/v)
C10	Manganese (II) chloride 0.01 M - tri-Sodium citrate 0.1 M pH 5.6 1,6-Hexanediol 2.5 M - 🔍	pH 5.6	416.7 μl of 1,6-Hexanediol (6 M) 10.0 μl of Manganese (II) chloride (1 M) 21.1 μl of tri-Sodium citrate pH 3 (1.6 M) 41.4 μl of tri-Sodium citrate pH 6.5 (1.6 M) 510.8 μl of water (100 v/v)
C11	Sodium acetate 0.1 M pH 4.6 Sodium chloride 2 M - 🔍	pH 4.6	64.1 μl of Sodium acetate pH 4 (1 M) 35.9 μl of Sodium acetate pH 5.5 (1 M) 400.0 μl of Sodium chloride (5 M) 500.0 μl of water (100 v/v)
C12	Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 2-methyl-2,4-pentanediol BCC 30 v/v - 🔍	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 64.1 μl of Sodium acetate pH 4 (1 M) 35.9 μl of Sodium acetate pH 5.5 (1 M) 40.0 μl of Sodium chloride (5 M) 560.0 μl of water (100 v/v)
D1	Cobalt (II) chloride 0.01 M - Sodium acetate 0.1 M pH 4.6 1,6-Hexanediol 1 M - 🔍	pH 4.6	166.7 μl of 1,6-Hexanediol (6 M) 10.0 μl of Cobalt (II) chloride (1 M) 64.1 μl of Sodium acetate pH 4 (1 M) 35.9 μl of Sodium acetate pH 5.5 (1 M) 723.3 μl of water (100 v/v)
D2	Cadmium chloride 0.1 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol 400 30 v/v - 🔍	pH 4.6	100.0 μl of Cadmium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 64.1 μl of Sodium acetate pH 4 (1 M) 35.9 μl of Sodium acetate pH 5.5 (1 M) 500.0 μl of water (100 v/v)
D3	Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	pH 4.6	57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 64.1 μl of Sodium acetate pH 4 (1 M) 35.9 μl of Sodium acetate pH 5.5 (1 M) 242.9 μl of water (100 v/v)
D4	Sodium chloride 2 M - Polyethylene glycol 6,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of Sodium chloride (5 M) 400.0 μl of water (100 v/v)
D5	Cetyltrimethylammonium bromide 0.01 mM - Magnesium chloride 0.1 M - Sodium chloride 0.5 M - 🔍	NA	0.2 μl of Cetyltrimethylammonium bromide (50 mM) 50.0 μl of Magnesium chloride (2 M) 100.0 μl of Sodium chloride (5 M) 849.8 μl of water (100 v/v)
D6	Ethylene glycol 25 v/v - 🔍	NA	250.0 μl of Ethylene glycol (100 v/v) 750.0 μl of water (100 v/v)
D7	1,4-Dioxane 35 v/v - 🔍	NA	350.0 μl of 1,4-Dioxane (100 v/v) 650.0 μl of water (100 v/v)
D8	Ammonium sulfate 2 M - 2-Propanol 5 v/v - 🔍	NA	50.0 μl of 2-Propanol (100 v/v) 571.4 μl of Ammonium sulfate (3.5 M) 378.6 μl of water (100 v/v)
D9	Imidazole 1 M pH 7 🔍	pH 7	529.7 μl of Imidazole pH 6.2 (1 M) 470.3 μl of Imidazole pH 8 (1 M)
D10	Polyethylene glycol 8,000 10 w/v - Polyethylene glycol 1,000 10 w/v - 🔍	NA	200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v)
D11	Sodium chloride 1.5 M - Ethanol 10 v/v - 🔍	NA	100.0 μl of Ethanol (100 v/v) 300.0 μl of Sodium chloride (5 M) 600.0 μl of water (100 v/v)
D12	tri-Sodium citrate 1.6 M pH 6.5 🔍	pH 6.5	74.1 μl of tri-Sodium citrate pH 3 (1.6 M) 925.9 μl of tri-Sodium citrate pH 6.5 (1.6 M)
E1	Polyvinylpyrrolidone K15 15 w/v - 🔍	NA	300.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 700.0 μl of water (100 v/v)
E2	Urea 2 M - 🔍	NA	1000.0 μl of Urea (2 M)
E3		NA
E4		NA
E5		NA
E6		NA
E7		NA
E8		NA
E9		NA
E10		NA
E11		NA
E12		NA
F1		NA
F2		NA
F3		NA
F4		NA
F5		NA
F6		NA
F7		NA
F8		NA
F9		NA
F10		NA
F11		NA
F12		NA
G1		NA
G2		NA
G3		NA
G4		NA
G5		NA
G6		NA
G7		NA
G8		NA
G9		NA
G10		NA
G11		NA
G12		NA
H1		NA
H2		NA
H3		NA
H4		NA
H5		NA
H6		NA
H7		NA
H8		NA
H9		NA
H10		NA
H11		NA
H12		NA