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Supplemental Information

Screen Description

MemGold 2

Created 2012-09-20.

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MemGold 2

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MemGold 2

    Screen Structure

    MemGold 2

    Well Count 96
    Number of distinct ingredients 63
    Internal diversity (score mean dist) 0.940

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:36:32.566856 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemGold 2
    Buffer 120
    Organic (non-volatile) 33
    Polymer 80
    Salt 116

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemGold 2 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol monomethyl ether 500 14 v/v -
    • Magnesium chloride 0.2 M -
    • Cadmium chloride 0.005 M -
    • tris chloride BCC 0.1 M pH 7.5
    		pH 7.5
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    140 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    5 μl of Cadmium chloride (1 M)
    655 μl of water (100 v/v)
    A2
    • Polyethylene glycol 3,000 44 w/v -
    • Potassium acetate 0.1 M -
    • Potassium chloride 0.01 M -
    • tris chloride BCC 0.02 M pH 7
    		pH 7
    880 μl of Polyethylene glycol 3,000 (50 w/v)
    20 μl of Potassium acetate (5 M)
    20 μl of tris chloride BCC pH 7 (1 M)
    2 μl of Potassium chloride (4 M)
    77 μl of water (100 v/v)
    A3
    • Polyethylene glycol 1,450 10 w/v -
    • Magnesium sulfate 0.08 M -
    • Sodium chloride 0.02 M -
    • MES 0.02 M pH 6
    		pH 6
    4 μl of Sodium chloride (5 M)
    16 μl of MES pH 7.1 (0.5 M)
    200 μl of Polyethylene glycol 1,450 (50 w/v)
    23 μl of MES pH 5.2 (0.5 M)
    32 μl of Magnesium sulfate (2.5 M)
    724 μl of water (100 v/v)
    A4
    • Polyethylene glycol 1,450 8 w/v -
    • Calcium chloride 0.02 M -
    • Magnesium sulfate 0.04 M -
    • MES 0.02 M pH 6.5
    		pH 6.5
    30 μl of MES pH 7.1 (0.5 M)
    160 μl of Polyethylene glycol 1,450 (50 w/v)
    9 μl of MES pH 5.2 (0.5 M)
    10 μl of Calcium chloride (2 M)
    16 μl of Magnesium sulfate (2.5 M)
    774 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 32 v/v -
    • Nickel sulfate 0.05 M -
    • Lithium chloride 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    		pH 8.5
    5 μl of Lithium chloride (10 M)
    12 μl of Nickel sulfate (4 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    612 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,350 10 w/v -
    • di-Sodium hydrogen phosphate 0.1 M -
    • Potassium di-hydrogen phosphate 0.1 M -
    • BIS-TRIS propane 0.1 M pH 7.5
    		pH 7.5
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of di-Sodium hydrogen phosphate (4 M)
    50 μl of Potassium di-hydrogen phosphate (2 M)
    625 μl of water (100 v/v)
    A7
    • Polyethylene glycol 4,000 11.5 w/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • ADA 0.1 M pH 6.5
    		pH 6.5
    20 μl of Sodium chloride (5 M)
    50 μl of Lithium sulfate (2 M)
    100 μl of ADA pH 6.5 (1 M)
    230 μl of Polyethylene glycol 4,000 (50 w/v)
    600 μl of water (100 v/v)
    A8
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.1 M -
    • Lithium chloride 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    10 μl of Lithium chloride (10 M)
    41 μl of Sodium acetate pH 5 (1 M)
    100 μl of Cadmium chloride (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    58 μl of Sodium acetate pH 4 (1 M)
    490 μl of water (100 v/v)
    A9
    • Polyethylene glycol 2,000 20 w/v -
    • Ammonium sulfate 0.2 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 6
    		pH 6
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    57 μl of Ammonium sulfate (3.5 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    460 μl of water (100 v/v)
    A10
    • Polyethylene glycol 400 31 v/v -
    • Lithium sulfate 0.2 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7
    		pH 7
    20 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    100 μl of Lithium sulfate (2 M)
    310 μl of Polyethylene glycol 400 (100 v/v)
    470 μl of water (100 v/v)
    A11
    • Polyethylene glycol 400 32 v/v -
    • Ammonium di-hydrogen phosphate 0.2 M -
    • Ammonium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 4.5
    		pH 4.5
    8 μl of tri-Sodium citrate pH 3 (1.6 M)
    28 μl of Ammonium sulfate (3.5 M)
    54 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    508 μl of water (100 v/v)
    A12
    • Polyethylene glycol 4,000 14 w/v -
    • tri-Sodium citrate 0.05 M -
    • Potassium chloride 0.12 M -
    • BIS-TRIS 0.08 M pH 6
    		pH 6
    31 μl of tri-Sodium citrate (1.6 M)
    62 μl of BIS-TRIS pH 5.5 (1 M)
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    30 μl of Potassium chloride (4 M)
    17 μl of BIS-TRIS pH 7 (1 M)
    578 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,000 19 w/v -
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 0.1 M -
    • MES 0.01 M pH 6.5
    		pH 6.5
    380 μl of Polyethylene glycol 1,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    15 μl of MES pH 7.1 (0.5 M)
    4 μl of MES pH 5.2 (0.5 M)
    28 μl of Ammonium sulfate (3.5 M)
    551 μl of water (100 v/v)
    B2
    • Polyethylene glycol monomethyl ether 2,000 18 w/v -
    • Nickel sulfate 0.01 M -
    • tri-Sodium citrate 0.1 M pH 6
    		pH 6
    2 μl of Nickel sulfate (4 M)
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    360 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    575 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,350 3.5 w/v -
    • Magnesium chloride 0.02 M -
    • MES 0.02 M pH 6
    		pH 6
    10 μl of Magnesium chloride (2 M)
    16 μl of MES pH 7.1 (0.5 M)
    70 μl of Polyethylene glycol 3,350 (50 w/v)
    23 μl of MES pH 5.2 (0.5 M)
    880 μl of water (100 v/v)
    B4
    • Polyethylene glycol monomethyl ether 350 14 v/v -
    • Sodium chloride 0.02 M -
    • MES 0.05 M pH 5.5
    		pH 5.5
    140 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    4 μl of Sodium chloride (5 M)
    50 μl of MES pH 5.5 (1 M)
    806 μl of water (100 v/v)
    B5
    • Polyethylene glycol monomethyl ether 500 35 v/v -
    • Magnesium chloride 0.025 M -
    • MOPS 0.02 M pH 7
    		pH 7
    6 μl of MOPS pH 7.9 (1 M)
    12 μl of Magnesium chloride (2 M)
    350 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    13 μl of MOPS pH 6.5 (1 M)
    617 μl of water (100 v/v)
    B6
    • Polyethylene glycol 400 28 v/v -
    • Magnesium chloride 0.03 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    15 μl of Magnesium chloride (2 M)
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    505 μl of water (100 v/v)
    B7
    • Polyethylene glycol monomethyl ether 350 25 v/v -
    • Sodium chloride 0.04 M -
    • tris chloride BCC 0.04 M pH 8
    		pH 8
    250 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    8 μl of Sodium chloride (5 M)
    40 μl of tris chloride BCC pH 8 (1 M)
    702 μl of water (100 v/v)
    B8
    • 2-methyl-2,4-pentanediol BCC 36 v/v -
    • Magnesium acetate 0.04 M -
    • MES 0.1 M pH 6
    		pH 6
    360 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    40 μl of Magnesium acetate (1 M)
    400 μl of water (100 v/v)
    B9
    • Polyethylene glycol 8,000 11 w/v -
    • Zinc acetate 0.05 M -
    • ADA 0.05 M pH 6.3
    		pH 6.3
    22 μl of ADA pH 6 (1 M)
    27 μl of ADA pH 6.5 (1 M)
    50 μl of Zinc acetate (1 M)
    220 μl of Polyethylene glycol 8,000 (50 w/v)
    680 μl of water (100 v/v)
    B10
    • Polyethylene glycol 400 26 v/v -
    • Magnesium acetate 0.05 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    50 μl of Magnesium acetate (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    490 μl of water (100 v/v)
    B11
    • Polyethylene glycol 400 32 v/v -
    • Magnesium acetate 0.05 M -
    • Glycine 0.1 M pH 9.5
    		pH 9.5
    50 μl of Magnesium acetate (1 M)
    58 μl of Glycine pH 9 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    41 μl of Glycine pH 10 (1 M)
    530 μl of water (100 v/v)
    B12
    • Polyethylene glycol 4,000 3 w/v -
    • Sodium chloride 0.066 M -
    • tris chloride BCC 0.02 M pH 7.5
    		pH 7.5
    13 μl of Sodium chloride (5 M)
    20 μl of tris chloride BCC pH 7.5 (1 M)
    60 μl of Polyethylene glycol 4,000 (50 w/v)
    906 μl of water (100 v/v)
    C1
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Magnesium chloride 0.075 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    37 μl of Magnesium chloride (2 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    262 μl of water (100 v/v)
    C2
    • Polyethylene glycol monomethyl ether 5,000 14 w/v -
    • Magnesium acetate 0.08 M -
    • tri-Sodium citrate 0.1 M pH 6
    		pH 6
    280 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    80 μl of Magnesium acetate (1 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    577 μl of water (100 v/v)
    C3
    • Ammonium sulfate 2 M -
    • Zinc acetate 0.05 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    50 μl of Zinc acetate (1 M)
    117 μl of MES pH 5.2 (0.5 M)
    571 μl of Ammonium sulfate (3.5 M)
    178 μl of water (100 v/v)
    C4
    • Polyethylene glycol dimethyl ether 250 22 v/v -
    • Ammonium sulfate 0.087 M -
    • tris chloride BCC 0.5 M pH 7
    		pH 7
    440 μl of Polyethylene glycol dimethyl ether 250 (50 v/v)
    500 μl of tris chloride BCC pH 7 (1 M)
    24 μl of Ammonium sulfate (3.5 M)
    35 μl of water (100 v/v)
    C5
    • Polyethylene glycol 8,000 13 w/v -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 7.5
    		pH 7.5
    50 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    260 μl of Polyethylene glycol 8,000 (50 w/v)
    590 μl of water (100 v/v)
    C6
    • Polyethylene glycol 3,350 17 w/v -
    • Magnesium formate 0.1 M -
    • MOPS 0.1 M pH 7
    		pH 7
    33 μl of MOPS pH 7.9 (1 M)
    340 μl of Polyethylene glycol 3,350 (50 w/v)
    67 μl of MOPS pH 6.5 (1 M)
    100 μl of Magnesium formate (1 M)
    460 μl of water (100 v/v)
    C7
    • Polyethylene glycol 4,000 18 w/v -
    • Potassium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6
    		pH 6
    36 μl of BIS-TRIS pH 6.5 (1 M)
    63 μl of BIS-TRIS pH 5.5 (1 M)
    360 μl of Polyethylene glycol 4,000 (50 w/v)
    25 μl of Potassium chloride (4 M)
    515 μl of water (100 v/v)
    C8
    • Polyethylene glycol 200 18 v/v -
    • Potassium chloride 0.1 M -
    • Potassium di-hydrogen phosphate 0.1 M pH 7.5
    		pH 7.5
    50 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
    180 μl of Polyethylene glycol 200 (100 v/v)
    25 μl of Potassium chloride (4 M)
    745 μl of water (100 v/v)
    C9
    • Polyethylene glycol 4,000 22 w/v -
    • Magnesium acetate 0.1 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    100 μl of Magnesium acetate (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    260 μl of water (100 v/v)
    C10
    • Polyethylene glycol 8,000 22 w/v -
    • Calcium acetate 0.1 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Calcium acetate (2 M)
    440 μl of Polyethylene glycol 8,000 (50 w/v)
    310 μl of water (100 v/v)
    C11
    • Polyethylene glycol 3,350 23 w/v -
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 8.5
    		pH 8.5
    460 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 8.5 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    411 μl of water (100 v/v)
    C12
    • Polyethylene glycol 400 32 v/v -
    • Potassium chloride 0.1 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    25 μl of Potassium chloride (4 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    455 μl of water (100 v/v)
    D1
    • Polyethylene glycol 300 36 v/v -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    360 μl of Polyethylene glycol 300 (100 v/v)
    20 μl of Sodium chloride (5 M)
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    420 μl of water (100 v/v)
    D2
    • Polyethylene glycol monomethyl ether 500 45 v/v -
    • Sodium chloride 0.1 M -
    • BICINE 0.1 M pH 9
    		pH 9
    20 μl of Sodium chloride (5 M)
    9 μl of BICINE pH 7.4 (1 M)
    450 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    90 μl of BICINE pH 9.3 (1 M)
    430 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 35 v/v -
    • Calcium chloride 0.15 M -
    • Glycine 0.1 M pH 9
    		pH 9
    100 μl of Glycine pH 9 (1 M)
    350 μl of Polyethylene glycol 400 (100 v/v)
    75 μl of Calcium chloride (2 M)
    475 μl of water (100 v/v)
    D4
    • Polyethylene glycol 4,000 13 w/v -
    • Ammonium sulfate 0.2 M -
    • ADA 0.5 M pH 6.5
    		pH 6.5
    500 μl of ADA pH 6.5 (1 M)
    260 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    182 μl of water (100 v/v)
    D5
    • Polyethylene glycol monomethyl ether 2,000 14 w/v -
    • Choline chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 7.5
    		pH 7.5
    100 μl of tris chloride BCC pH 7.5 (1 M)
    200 μl of Choline chloride (1 M)
    280 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    420 μl of water (100 v/v)
    D6
    • Polyethylene glycol 6,000 19 w/v -
    • Sodium chloride 0.2 M -
    • MOPS 0.05 M pH 7
    		pH 7
    16 μl of MOPS pH 7.9 (1 M)
    40 μl of Sodium chloride (5 M)
    380 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of MOPS pH 6.5 (1 M)
    530 μl of water (100 v/v)
    D7
    • Polyethylene glycol 6,000 19 w/v -
    • Sodium chloride 0.05 M -
    • MOPS 0.05 M pH 7
    		pH 7
    16 μl of MOPS pH 7.9 (1 M)
    10 μl of Sodium chloride (5 M)
    380 μl of Polyethylene glycol 6,000 (50 w/v)
    33 μl of MOPS pH 6.5 (1 M)
    560 μl of water (100 v/v)
    D8
    • Polyethylene glycol 3,350 19 w/v -
    • Magnesium formate 0.2 M -
    • tris chloride BCC 0.05 M pH 8
    		pH 8
    380 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Magnesium formate (1 M)
    370 μl of water (100 v/v)
    D9
    • Polyethylene glycol monomethyl ether 350 20 v/v -
    • Calcium chloride 0.2 M -
    • MES 0.1 M pH 5
    		pH 5
    200 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    100 μl of MES pH 5 (1 M)
    100 μl of Calcium chloride (2 M)
    600 μl of water (100 v/v)
    D10
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium nitrate 0.2 M -
    • HEPES 0.05 M pH 7
    		pH 7
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    100 μl of Ammonium nitrate (2 M)
    450 μl of water (100 v/v)
    D11
    • Polyethylene glycol 1,000 33 w/v -
    • Magnesium chloride 0.5 M -
    • Lithium chloride 0.02 M -
    • Glycine 0.02 M pH 10
    		pH 10
    660 μl of Polyethylene glycol 1,000 (50 w/v)
    2 μl of Lithium chloride (10 M)
    250 μl of Magnesium chloride (2 M)
    20 μl of Glycine pH 10 (1 M)
    68 μl of water (100 v/v)
    D12
    • Polyethylene glycol 400 24 v/v -
    • Calcium acetate 0.2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    100 μl of Calcium acetate (2 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    560 μl of water (100 v/v)
    E1
    • Polyethylene glycol 400 28 v/v -
    • Sodium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    66 μl of Sodium acetate (3 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    453 μl of water (100 v/v)
    E2
    • Polyethylene glycol 400 29 v/v -
    • Sodium chloride 0.2 M -
    • Calcium acetate 0.05 M pH 5
    		pH 5
    40 μl of Sodium chloride (5 M)
    50 μl of Calcium acetate pH 5 (1 M)
    290 μl of Polyethylene glycol 400 (100 v/v)
    620 μl of water (100 v/v)
    E3
    • Polyethylene glycol 400 29 v/v -
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7
    		pH 7
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    290 μl of Polyethylene glycol 400 (100 v/v)
    570 μl of water (100 v/v)
    E4
    • Pentaerythritol ethoxylate (15/4 EO/OH) 31 v/v -
    • Ammonium formate 0.2 M -
    • tris chloride BCC 0.1 M pH 7
    		pH 7
    20 μl of Ammonium formate (10 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    620 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    260 μl of water (100 v/v)
    E5
    • Polyethylene glycol 3,350 35 w/v -
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    700 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    142 μl of water (100 v/v)
    E6
    • Polyethylene glycol 400 38 v/v -
    • Calcium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 5
    		pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    100 μl of Calcium acetate (2 M)
    380 μl of Polyethylene glycol 400 (100 v/v)
    420 μl of water (100 v/v)
    E7
    • Polyethylene glycol 400 38 v/v -
    • Sodium chloride 0.2 M -
    • MOPS 0.1 M pH 7.5
    		pH 7.5
    74 μl of MOPS pH 7.9 (1 M)
    40 μl of Sodium chloride (5 M)
    25 μl of MOPS pH 6.5 (1 M)
    380 μl of Polyethylene glycol 400 (100 v/v)
    480 μl of water (100 v/v)
    E8
    • Ammonium sulfate 2 M -
    • Sodium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    571 μl of Ammonium sulfate (3.5 M)
    288 μl of water (100 v/v)
    E9
    • Polyethylene glycol 4,000 12 w/v -
    • Ammonium sulfate 0.225 M -
    • Sodium acetate 0.05 M pH 4
    		pH 4
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    64 μl of Ammonium sulfate (3.5 M)
    50 μl of Sodium acetate pH 4 (1 M)
    645 μl of water (100 v/v)
    E10
    • Polyethylene glycol 400 33 v/v -
    • Sodium chloride 0.23 M -
    • Sodium acetate 0.05 M pH 4.5
    		pH 4.5
    46 μl of Sodium chloride (5 M)
    20 μl of Sodium acetate pH 5 (1 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    29 μl of Sodium acetate pH 4 (1 M)
    574 μl of water (100 v/v)
    E11
    • Polyethylene glycol 3,000 22 w/v -
    • Magnesium formate 0.25 M -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    440 μl of Polyethylene glycol 3,000 (50 w/v)
    250 μl of Magnesium formate (1 M)
    210 μl of water (100 v/v)
    E12
    • Polyethylene glycol 1,000 40 w/v -
    • Magnesium chloride 0.25 M -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    800 μl of Polyethylene glycol 1,000 (50 w/v)
    62 μl of Magnesium chloride (4 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    37 μl of water (100 v/v)
    F1
    • Polyethylene glycol 400 25 v/v -
    • Lithium sulfate 0.3 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    150 μl of Lithium sulfate (2 M)
    48 μl of MES pH 5.2 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    F2
    • Polyethylene glycol monomethyl ether 500 33 v/v -
    • Ammonium formate 0.3 M -
    • tris chloride BCC 0.05 M pH 9
    		pH 9
    30 μl of Ammonium formate (10 M)
    330 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of tris chloride BCC pH 9 (1 M)
    590 μl of water (100 v/v)
    F3
    • Polyethylene glycol 400 34 v/v -
    • Barium chloride 0.3 M -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Barium chloride (1 M)
    340 μl of Polyethylene glycol 400 (100 v/v)
    160 μl of water (100 v/v)
    F4
    • Polyethylene glycol 4,000 14 w/v -
    • Lithium chloride 0.32 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    32 μl of Lithium chloride (10 M)
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    625 μl of water (100 v/v)
    F5
    • Polyethylene glycol 4,000 12 w/v -
    • Ammonium sulfate 0.34 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    		pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    97 μl of Ammonium sulfate (3.5 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    600 μl of water (100 v/v)
    F6
    • Polyethylene glycol 600 11 v/v -
    • Lithium sulfate 0.35 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    110 μl of Polyethylene glycol 600 (100 v/v)
    41 μl of Sodium acetate pH 5 (1 M)
    175 μl of Lithium sulfate (2 M)
    58 μl of Sodium acetate pH 4 (1 M)
    615 μl of water (100 v/v)
    F7
    • Polyethylene glycol 400 22 v/v -
    • Potassium nitrate 0.37 M -
    		NA
    123 μl of Potassium nitrate (3 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    656 μl of water (100 v/v)
    F8
    • Polyethylene glycol 3,350 10 w/v -
    • Ammonium sulfate 0.4 M -
    • MES 0.1 M pH 6.5
    		pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    48 μl of MES pH 5.2 (0.5 M)
    114 μl of Ammonium sulfate (3.5 M)
    485 μl of water (100 v/v)
    F9
    • Polyethylene glycol 400 32 v/v -
    • Sodium chloride 0.05 M -
    • Magnesium chloride 0.04 M -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    20 μl of Magnesium chloride (2 M)
    10 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    F10
    • Polyethylene glycol 400 12 v/v -
    • Potassium chloride 0.4 M -
    • HEPES 0.05 M pH 7.5
    		pH 7.5
    50 μl of HEPES pH 7.5 (1 M)
    100 μl of Potassium chloride (4 M)
    120 μl of Polyethylene glycol 400 (100 v/v)
    730 μl of water (100 v/v)
    F11
    • Polyethylene glycol 4,000 15 w/v -
    • Ammonium thiocyanate 0.4 M -
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    41 μl of Sodium acetate pH 5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of Ammonium thiocyanate (1 M)
    58 μl of Sodium acetate pH 4 (1 M)
    200 μl of water (100 v/v)
    F12
    • Polyethylene glycol 4,000 16 w/v -
    • Sodium thiocyanate 0.4 M -
    • Sodium acetate 0.1 M pH 4
    		pH 4
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Sodium thiocyanate (8 M)
    100 μl of Sodium acetate pH 4 (1 M)
    530 μl of water (100 v/v)
    G1
    • Polyethylene glycol 400 20 v/v -
    • Potassium chloride 0.5 M -
    • HEPES 0.05 M pH 6.5
    		pH 6.5
    18 μl of HEPES pH 7 (1 M)
    31 μl of HEPES pH 5 (1 M)
    125 μl of Potassium chloride (4 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    625 μl of water (100 v/v)
    G2
    • Polyethylene glycol monomethyl ether 350 21 v/v -
    • Magnesium chloride 0.5 M -
    • tris chloride BCC 0.05 M pH 7.5
    		pH 7.5
    210 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    250 μl of Magnesium chloride (2 M)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    490 μl of water (100 v/v)
    G3
    • Polyethylene glycol 4,000 11 w/v -
    • Potassium formate 0.8 M -
    • Sodium acetate 0.1 M pH 5
    		pH 5
    100 μl of Sodium acetate pH 5 (1 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    57 μl of Potassium formate (14 M)
    622 μl of water (100 v/v)
    G4
    • Polyethylene glycol 8,000 9 w/v -
    • MOPS 0.1 M pH 7
    		pH 7
    33 μl of MOPS pH 7.9 (1 M)
    67 μl of MOPS pH 6.5 (1 M)
    180 μl of Polyethylene glycol 8,000 (50 w/v)
    720 μl of water (100 v/v)
    G5
    • Polyethylene glycol 20,000 11 w/v -
    • MES 0.1 M pH 6
    		pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    366 μl of Polyethylene glycol 20,000 (30 w/v)
    433 μl of water (100 v/v)
    G6
    • Polyethylene glycol 400 13 v/v -
    • MES 0.1 M pH 6.5
    		pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    130 μl of Polyethylene glycol 400 (100 v/v)
    670 μl of water (100 v/v)
    G7
    • Polyethylene glycol 6,000 14 w/v -
    • ADA 0.1 M pH 5.5
    		pH 5.5
    100 μl of ADA pH 5.5 (1 M)
    280 μl of Polyethylene glycol 6,000 (50 w/v)
    620 μl of water (100 v/v)
    G8
    • Polyethylene glycol monomethyl ether 350 17 v/v -
    • tris chloride BCC 0.05 M pH 7.5
    		pH 7.5
    170 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    50 μl of tris chloride BCC pH 7.5 (1 M)
    780 μl of water (100 v/v)
    G9
    • Polyethylene glycol 300 22 v/v -
    • tri-Sodium citrate 0.07 M pH 4.5
    		pH 4.5
    220 μl of Polyethylene glycol 300 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    38 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    736 μl of water (100 v/v)
    G10
    • Polyethylene glycol 400 24 v/v -
    • ADA 0.05 M pH 6.5
    		pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    710 μl of water (100 v/v)
    G11
    • Polyethylene glycol 1,500 24 w/v -
    • Sodium cacodylate 0.1 M pH 6.5
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    420 μl of water (100 v/v)
    G12
    • Polyethylene glycol 600 28 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    280 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of water (100 v/v)
    H1
    • Polyethylene glycol 400 28 v/v -
    • tris chloride BCC 0.05 M pH 8.5
    		pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    670 μl of water (100 v/v)
    H2
    • Polyethylene glycol 400 30 v/v -
    • BICINE 0.1 M pH 9
    		pH 9
    9 μl of BICINE pH 7.4 (1 M)
    90 μl of BICINE pH 9.3 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    H3
    • Polyethylene glycol 600 31 v/v -
    • ADA 0.1 M pH 7
    		pH 7
    310 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of ADA pH 7 (1 M)
    590 μl of water (100 v/v)
    H4
    • Polyethylene glycol monomethyl ether 500 32 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    320 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    580 μl of water (100 v/v)
    H5
    • Polyethylene glycol 400 33 v/v -
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    570 μl of water (100 v/v)
    H6
    • Polyethylene glycol 3,350 34 w/v -
    • tri-Sodium citrate 0.18 M pH 4
    		pH 4
    680 μl of Polyethylene glycol 3,350 (50 w/v)
    19 μl of tri-Sodium citrate pH 3 (1.6 M)
    93 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    207 μl of water (100 v/v)
    H7
    • Polyethylene glycol 200 44 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    		pH 8.5
    440 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    460 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    250 μl of water (100 v/v)
    H9
    • Ammonium chloride 2.75 M -
    • BIS-TRIS 0.025 M pH 7
    		pH 7
    550 μl of Ammonium chloride (5 M)
    25 μl of BIS-TRIS pH 7 (1 M)
    425 μl of water (100 v/v)
    H10
    • Ammonium chloride 2.8 M -
    • HEPES 0.075 M pH 7.5
    		pH 7.5
    560 μl of Ammonium chloride (5 M)
    75 μl of HEPES pH 7.5 (1 M)
    365 μl of water (100 v/v)
    H11
    • Ammonium sulfate 3 M -
    • MES 0.1 M pH 5.5
    		pH 5.5
    857 μl of Ammonium sulfate (3.5 M)
    100 μl of MES pH 5.5 (1 M)
    42 μl of water (100 v/v)
    H12
    • 1,6-Hexanediol 3.25 M -
    • HEPES 0.01 M pH 7.5
    		pH 7.5
    541 μl of 1,6-Hexanediol (6 M)
    10 μl of HEPES pH 7.5 (1 M)
    448 μl of water (100 v/v)