C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard I

Wizard / Wizard I

Created 2013-01-04.

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Wizard I

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Wizard I

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Screen Structure

Wizard I

Well Count	48
Number of distinct ingredients	31
Internal diversity (score mean dist)	0.943

Screen Composition

More information about screen composition chart.

ingredient type	Wizard I
Buffer	58
Organic (non-volatile)	6
Organic (volatile)	8
Polymer	22
Salt	58

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard I Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 20 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
A2	2-Propanol 10 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v)
A3	Ethanol 15 v/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 300.0 μl of Ethanol (50 v/v) 600.0 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 35 v/v - Imidazole 0.1 M pH 8 Magnesium chloride 0.2 M - 🔍	pH 8	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 470.0 μl of water (100 v/v)
A5	Polyethylene glycol 400 30 v/v - CAPS 0.1 M pH 10 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 600.0 μl of water (100 v/v)
A6	Polyethylene glycol 3,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	400.0 μl of Polyethylene glycol 3,000 (50 w/v) 25.0 μl of tri-Sodium citrate pH 5.5 (4 M) 575.0 μl of water (100 v/v)
A7	Polyethylene glycol 8,000 10 w/v - MES 0.1 M pH 6 Zinc acetate 0.2 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Zinc acetate (2 M) 600.0 μl of water (100 v/v)
A8	Ammonium sulfate 2 M - tri-Sodium citrate 0.1 M pH 5.5 🔍	pH 5.5	666.7 μl of Ammonium sulfate (3 M) 25.0 μl of tri-Sodium citrate pH 5.5 (4 M) 308.3 μl of water (100 v/v)
B1	di-Ammonium hydrogen phosphate 1 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	50.0 μl of Sodium acetate pH 4.5 (2 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 664.3 μl of water (100 v/v)
B2	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 33.3 μl of tris chloride BCC pH 7 (3 M) 566.7 μl of water (100 v/v)
B3	1,4-Butanediol 20 v/v - MES 0.1 M pH 6 Lithium sulfate 0.2 M - 🔍	pH 6	200.0 μl of 1,4-Butanediol (100 v/v) 80.0 μl of Lithium sulfate (2.5 M) 100.0 μl of MES pH 6 (1 M) 620.0 μl of water (100 v/v)
B4	Polyethylene glycol 1,000 20 w/v - Imidazole 0.1 M pH 8 Calcium acetate 0.2 M - 🔍	pH 8	200.0 μl of Calcium acetate (1 M) 100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 300.0 μl of water (100 v/v)
B5	Ammonium sulfate 1.26 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	420.0 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 480.0 μl of water (100 v/v)
B6	tri-Sodium citrate 1 M - Sodium cacodylate 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of Sodium cacodylate pH 6.5 (1 M) 500.0 μl of tri-Sodium citrate (2 M) 400.0 μl of water (100 v/v)
B7	Polyethylene glycol 3,000 10 w/v - Imidazole 0.1 M pH 8 Lithium sulfate 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 80.0 μl of Lithium sulfate (2.5 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 620.0 μl of water (100 v/v)
B8	Sodium chloride 2.5 M - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 500.0 μl of Sodium chloride (5 M) 475.0 μl of water (100 v/v)
C1	Polyethylene glycol 8,000 30 w/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 270.0 μl of water (100 v/v)
C2	Potassium Sodium tartrate 1 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Potassium Sodium tartrate (2 M) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v)
C3	Polyethylene glycol 1,000 20 w/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	400.0 μl of Polyethylene glycol 1,000 (50 w/v) 33.3 μl of tris chloride BCC pH 7 (3 M) 566.7 μl of water (100 v/v)
C4	Sodium di-hydrogen phosphate 0.4 M - di-Potassium hydrogen phosphate 1.6 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium di-hydrogen phosphate (4 M) 320.0 μl of di-Potassium hydrogen phosphate (5 M) 440.0 μl of water (100 v/v)
C5	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
C6	2-Propanol 10 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of 2-Propanol (100 v/v) 33.3 μl of tris chloride BCC pH 8.5 (3 M) 866.7 μl of water (100 v/v)
C7	Ethanol 15 v/v - Imidazole 0.1 M pH 8 Magnesium chloride 0.2 M - 🔍	pH 8	300.0 μl of Ethanol (50 v/v) 100.0 μl of Imidazole pH 8 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 520.0 μl of water (100 v/v)
C8	2-methyl-2,4-pentanediol BCC 35 v/v - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Sodium chloride (5 M) 33.3 μl of tris chloride BCC pH 7 (3 M) 576.7 μl of water (100 v/v)
D1	Polyethylene glycol 400 30 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v)
D2	Polyethylene glycol 3,000 10 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 700.0 μl of water (100 v/v)
D3	Sodium di-hydrogen phosphate 1.2 M - di-Potassium hydrogen phosphate 0.8 M - CAPS 0.1 M pH 10 Lithium sulfate 0.2 M - 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 80.0 μl of Lithium sulfate (2.5 M) 300.0 μl of Sodium di-hydrogen phosphate (4 M) 160.0 μl of di-Potassium hydrogen phosphate (5 M) 360.0 μl of water (100 v/v)
D4	Polyethylene glycol 3,000 20 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 460.0 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 10 w/v - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 660.0 μl of water (100 v/v)
D6	Ammonium sulfate 1.26 M - Sodium acetate 0.1 M pH 4.5 Sodium chloride 0.2 M - 🔍	pH 4.5	420.0 μl of Ammonium sulfate (3 M) 50.0 μl of Sodium acetate pH 4.5 (2 M) 40.0 μl of Sodium chloride (5 M) 490.0 μl of water (100 v/v)
D7	Polyethylene glycol 8,000 20 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	400.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 460.0 μl of water (100 v/v)
D8	Polyethylene glycol 3,000 10 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	200.0 μl of Polyethylene glycol 3,000 (50 w/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 775.0 μl of water (100 v/v)
E1	Ammonium sulfate 2 M - CAPS 0.1 M pH 10 Lithium sulfate 0.2 M - 🔍	pH 10	666.7 μl of Ammonium sulfate (3 M) 100.0 μl of CAPS pH 10 (1 M) 80.0 μl of Lithium sulfate (2.5 M) 153.3 μl of water (100 v/v)
E2	di-Ammonium hydrogen phosphate 1 M - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 285.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 614.3 μl of water (100 v/v)
E3	1,4-Butanediol 20 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200.0 μl of 1,4-Butanediol (100 v/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 750.0 μl of water (100 v/v)
E4	tri-Sodium citrate 1 M - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of tri-Sodium citrate (2 M) 400.0 μl of water (100 v/v)
E5	Sodium chloride 2.5 M - Imidazole 0.1 M pH 8 🔍	pH 8	100.0 μl of Imidazole pH 8 (1 M) 500.0 μl of Sodium chloride (5 M) 400.0 μl of water (100 v/v)
E6	Potassium Sodium tartrate 1 M - CHES 0.1 M pH 9.5 Lithium sulfate 0.2 M - 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 80.0 μl of Lithium sulfate (2.5 M) 500.0 μl of Potassium Sodium tartrate (2 M) 320.0 μl of water (100 v/v)
E7	Polyethylene glycol 1,000 20 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Lithium sulfate 0.2 M - 🔍	pH 4.2	80.0 μl of Lithium sulfate (2.5 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 420.0 μl of water (100 v/v)
E8	2-Propanol 10 v/v - MES 0.1 M pH 6 Calcium acetate 0.2 M - 🔍	pH 6	100.0 μl of 2-Propanol (100 v/v) 200.0 μl of Calcium acetate (1 M) 100.0 μl of MES pH 6 (1 M) 600.0 μl of water (100 v/v)
F1	Polyethylene glycol 3,000 30 w/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 300.0 μl of water (100 v/v)
F2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 7 🔍	pH 7	300.0 μl of Ethanol (50 v/v) 33.3 μl of tris chloride BCC pH 7 (3 M) 666.7 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium Potassium phosphate 0.1 M pH 6.2 🔍	pH 6.2	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 25.0 μl of Sodium Potassium phosphate pH 6.2 (4 M) 625.0 μl of water (100 v/v)
F4	Polyethylene glycol 400 30 v/v - Sodium acetate 0.1 M pH 4.5 Calcium acetate 0.2 M - 🔍	pH 4.5	200.0 μl of Calcium acetate (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 450.0 μl of water (100 v/v)
F5	Polyethylene glycol 3,000 20 w/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	400.0 μl of Polyethylene glycol 3,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 550.0 μl of water (100 v/v)
F6	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 Calcium acetate 0.2 M - 🔍	pH 8	200.0 μl of Calcium acetate (1 M) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
F7	Ammonium sulfate 1.26 M - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	420.0 μl of Ammonium sulfate (3 M) 80.0 μl of Lithium sulfate (2.5 M) 33.3 μl of tris chloride BCC pH 8.5 (3 M) 466.7 μl of water (100 v/v)
F8	Polyethylene glycol 1,000 20 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	400.0 μl of Polyethylene glycol 1,000 (50 w/v) 50.0 μl of Sodium acetate pH 4.5 (2 M) 100.0 μl of Zinc acetate (2 M) 450.0 μl of water (100 v/v)