C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Wizard II

Wizard / Wizard II

Created 2013-01-04.

Export

Wizard II

Barcodes

Wizard II

Screen Structure

Wizard II

Well Count	48
Number of distinct ingredients	30
Internal diversity (score mean dist)	0.928

Screen Composition

More information about screen composition chart.

ingredient type	Wizard II
Buffer	56
Organic (non-volatile)	6
Organic (volatile)	8
Polymer	19
Salt	72

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard II Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,000 10 w/v - Sodium acetate 0.1 M pH 4.5 Zinc acetate 0.2 M - 🔍	pH 4.5	200 μl of Polyethylene glycol 3,000 (50 w/v) 200 μl of Zinc acetate (1 M) 50 μl of Sodium acetate pH 4.5 (2 M) 550 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 35 v/v - MES 0.1 M pH 6 Lithium sulfate 0.2 M - 🔍	pH 6	80 μl of Lithium sulfate (2.5 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 33 μl of MES pH 6 (3 M) 536 μl of water (100 v/v)
A3	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 420 μl of water (100 v/v)
A4	Ammonium sulfate 2 M - Sodium cacodylate 0.1 M pH 6.5 Sodium chloride 0.2 M - 🔍	pH 6.5	40 μl of Sodium chloride (5 M) 666 μl of Ammonium sulfate (3 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 193 μl of water (100 v/v)
A5	1,4-Butanediol 20 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	200 μl of 1,4-Butanediol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 40 μl of Sodium chloride (5 M) 660 μl of water (100 v/v)
A6	2-Propanol 10 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Lithium sulfate 0.2 M - 🔍	pH 4.2	80 μl of Lithium sulfate (2.5 M) 100 μl of 2-Propanol (100 v/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 720 μl of water (100 v/v)
A7	Polyethylene glycol 3,000 30 w/v - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	600 μl of Polyethylene glycol 3,000 (50 w/v) 40 μl of Sodium chloride (5 M) 33 μl of tris chloride BCC pH 7 (3 M) 326 μl of water (100 v/v)
A8	Polyethylene glycol 8,000 10 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Sodium chloride (5 M) 735 μl of water (100 v/v)
B1	Ammonium sulfate 2 M - Sodium phosphate citrate 0.1 M pH 4.2 🔍	pH 4.2	100 μl of Sodium phosphate citrate pH 4.2 (1 M) 666 μl of Ammonium sulfate (3 M) 233 μl of water (100 v/v)
B2	di-Ammonium hydrogen phosphate 1 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 614 μl of water (100 v/v)
B3	2-Propanol 10 v/v - Sodium cacodylate 0.1 M pH 6.5 Zinc acetate 0.2 M - 🔍	pH 6.5	100 μl of Zinc acetate (2 M) 100 μl of 2-Propanol (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 700 μl of water (100 v/v)
B4	Polyethylene glycol 400 30 v/v - Sodium cacodylate 0.1 M pH 6.5 Lithium sulfate 0.2 M - 🔍	pH 6.5	80 μl of Lithium sulfate (2.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 520 μl of water (100 v/v)
B5	Ethanol 15 v/v - tri-Sodium citrate 0.1 M pH 5.5 Lithium sulfate 0.2 M - 🔍	pH 5.5	80 μl of Lithium sulfate (2.5 M) 25 μl of tri-Sodium citrate pH 5.5 (4 M) 300 μl of Ethanol (50 v/v) 595 μl of water (100 v/v)
B6	Polyethylene glycol 1,000 20 w/v - Sodium Potassium phosphate 0.1 M pH 6.2 Sodium chloride 0.2 M - 🔍	pH 6.2	400 μl of Polyethylene glycol 1,000 (50 w/v) 25 μl of Sodium Potassium phosphate pH 6.2 (4 M) 40 μl of Sodium chloride (5 M) 535 μl of water (100 v/v)
B7	Ammonium sulfate 1.26 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 420 μl of Ammonium sulfate (3 M) 480 μl of water (100 v/v)
B8	tri-Sodium citrate 1 M - CHES 0.1 M pH 9.5 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 500 μl of tri-Sodium citrate (2 M) 400 μl of water (100 v/v)
C1	Sodium chloride 2.5 M - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	80 μl of Magnesium chloride (2.5 M) 500 μl of Sodium chloride (5 M) 33 μl of tris chloride BCC pH 7 (3 M) 386 μl of water (100 v/v)
C2	Polyethylene glycol 3,000 20 w/v - tris chloride BCC 0.1 M pH 7 Calcium acetate 0.2 M - 🔍	pH 7	400 μl of Polyethylene glycol 3,000 (50 w/v) 200 μl of Calcium acetate (1 M) 33 μl of tris chloride BCC pH 7 (3 M) 366 μl of water (100 v/v)
C3	Sodium di-hydrogen phosphate 1.6 M - Sodium phosphate citrate 0.1 M pH 4.2 di-Potassium hydrogen phosphate 0.4 M - 🔍	pH 4.2	400 μl of Sodium di-hydrogen phosphate (4 M) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 80 μl of di-Potassium hydrogen phosphate (5 M) 420 μl of water (100 v/v)
C4	Ethanol 15 v/v - MES 0.1 M pH 6 Zinc acetate 0.2 M - 🔍	pH 6	100 μl of Zinc acetate (2 M) 33 μl of MES pH 6 (3 M) 300 μl of Ethanol (50 v/v) 566 μl of water (100 v/v)
C5	2-methyl-2,4-pentanediol BCC 35 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of Sodium acetate pH 4.5 (2 M) 600 μl of water (100 v/v)
C6	2-Propanol 10 v/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of 2-Propanol (100 v/v) 800 μl of water (100 v/v)
C7	Ethanol 15 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 300 μl of Ethanol (50 v/v) 520 μl of water (100 v/v)
C8	Polyethylene glycol 8,000 30 w/v - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Sodium chloride (5 M) 260 μl of water (100 v/v)
D1	2-methyl-2,4-pentanediol BCC 35 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40 μl of Sodium chloride (5 M) 510 μl of water (100 v/v)
D2	Polyethylene glycol 400 30 v/v - CHES 0.1 M pH 9.5 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 300 μl of Polyethylene glycol 400 (100 v/v) 600 μl of water (100 v/v)
D3	Polyethylene glycol 3,000 10 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	200 μl of Polyethylene glycol 3,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 620 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 20 w/v - MES 0.1 M pH 6 Calcium acetate 0.2 M - 🔍	pH 6	33 μl of MES pH 6 (3 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Calcium acetate (1 M) 366 μl of water (100 v/v)
D5	Ammonium sulfate 1.26 M - CHES 0.1 M pH 9.5 Sodium chloride 0.2 M - 🔍	pH 9.5	100 μl of CHES pH 9.5 (1 M) 40 μl of Sodium chloride (5 M) 420 μl of Ammonium sulfate (3 M) 440 μl of water (100 v/v)
D6	1,4-Butanediol 20 v/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of Zinc acetate (2 M) 200 μl of 1,4-Butanediol (100 v/v) 600 μl of water (100 v/v)
D7	tri-Sodium citrate 1 M - tris chloride BCC 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	40 μl of Sodium chloride (5 M) 33 μl of tris chloride BCC pH 7 (3 M) 500 μl of tri-Sodium citrate (2 M) 426 μl of water (100 v/v)
D8	Polyethylene glycol 1,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
E1	di-Ammonium hydrogen phosphate 1 M - tri-Sodium citrate 0.1 M pH 5.5 Sodium chloride 0.2 M - 🔍	pH 5.5	25 μl of tri-Sodium citrate pH 5.5 (4 M) 40 μl of Sodium chloride (5 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 649 μl of water (100 v/v)
E2	Polyethylene glycol 8,000 10 w/v - Imidazole 0.1 M pH 8 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v)
E3	Sodium di-hydrogen phosphate 0.8 M - di-Potassium hydrogen phosphate 1.2 M - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	200 μl of Sodium di-hydrogen phosphate (4 M) 240 μl of di-Potassium hydrogen phosphate (5 M) 50 μl of Sodium acetate pH 4.5 (2 M) 510 μl of water (100 v/v)
E4	Polyethylene glycol 3,000 10 w/v - Sodium phosphate citrate 0.1 M pH 4.2 Sodium chloride 0.2 M - 🔍	pH 4.2	200 μl of Polyethylene glycol 3,000 (50 w/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 40 μl of Sodium chloride (5 M) 660 μl of water (100 v/v)
E5	Potassium Sodium tartrate 1 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	500 μl of Potassium Sodium tartrate (2 M) 80 μl of Lithium sulfate (2.5 M) 33 μl of tris chloride BCC pH 7 (3 M) 386 μl of water (100 v/v)
E6	Sodium chloride 2.5 M - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	80 μl of Lithium sulfate (2.5 M) 500 μl of Sodium chloride (5 M) 50 μl of Sodium acetate pH 4.5 (2 M) 370 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 20 w/v - CAPS 0.1 M pH 10 Sodium chloride 0.2 M - 🔍	pH 10	400 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Sodium chloride (5 M) 100 μl of CAPS pH 10 (1 M) 460 μl of water (100 v/v)
E8	Polyethylene glycol 3,000 20 w/v - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of Zinc acetate (2 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 400 μl of water (100 v/v)
F1	Ammonium sulfate 2 M - tris chloride BCC 0.1 M pH 7 Lithium sulfate 0.2 M - 🔍	pH 7	80 μl of Lithium sulfate (2.5 M) 33 μl of tris chloride BCC pH 7 (3 M) 666 μl of Ammonium sulfate (3 M) 220 μl of water (100 v/v)
F2	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.2 M - 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 40 μl of Sodium chloride (5 M) 300 μl of Polyethylene glycol 400 (100 v/v) 560 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 10 w/v - tris chloride BCC 0.1 M pH 7 Magnesium chloride 0.2 M - 🔍	pH 7	200 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 33 μl of tris chloride BCC pH 7 (3 M) 686 μl of water (100 v/v)
F4	Polyethylene glycol 1,000 20 w/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	400 μl of Polyethylene glycol 1,000 (50 w/v) 80 μl of Magnesium chloride (2.5 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 420 μl of water (100 v/v)
F5	Ammonium sulfate 1.26 M - MES 0.1 M pH 6 🔍	pH 6	33 μl of MES pH 6 (3 M) 420 μl of Ammonium sulfate (3 M) 546 μl of water (100 v/v)
F6	di-Ammonium hydrogen phosphate 1 M - Imidazole 0.1 M pH 8 Sodium chloride 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 40 μl of Sodium chloride (5 M) 285 μl of di-Ammonium hydrogen phosphate (3.5 M) 574 μl of water (100 v/v)
F7	Sodium chloride 2.5 M - Imidazole 0.1 M pH 8 Zinc acetate 0.2 M - 🔍	pH 8	100 μl of Imidazole pH 8 (1 M) 100 μl of Zinc acetate (2 M) 500 μl of Sodium chloride (5 M) 300 μl of water (100 v/v)
F8	Potassium Sodium tartrate 1 M - MES 0.1 M pH 6 🔍	pH 6	500 μl of Potassium Sodium tartrate (2 M) 33 μl of MES pH 6 (3 M) 466 μl of water (100 v/v)