C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard III

Wizard / Wizard III

Created 2013-01-04.

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Wizard III

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Wizard III

Screen Structure

Wizard III

Well Count	48
Number of distinct ingredients	46
Internal diversity (score mean dist)	0.954

Screen Composition

More information about screen composition chart.

ingredient type	Wizard III
Buffer	45
Cryoprotectant	6
Organic (non-volatile)	17
Organic (volatile)	5
Polymer	34
Salt	43

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard III Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 3,350 20 w/v - di-Ammonium hydrogen citrate 0.2 M - 🔍	NA	80 μl of di-Ammonium hydrogen citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v)
A2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.02 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20 μl of Calcium chloride (1 M) 580 μl of water (100 v/v)
A3	Polyethylene glycol 3,350 20 w/v - Magnesium formate 0.2 M - 🔍	NA	200 μl of Magnesium formate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v)
A4	Polyethylene glycol 3,350 20 w/v - Ammonium formate 0.2 M - 🔍	NA	40 μl of Ammonium formate (5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 560 μl of water (100 v/v)
A5	Polyethylene glycol 3,350 20 w/v - Ammonium chloride 0.2 M - 🔍	NA	40 μl of Ammonium chloride (5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 560 μl of water (100 v/v)
A6	Polyethylene glycol 3,350 20 w/v - Barium chloride 0.2 M - 🔍	NA	200 μl of Barium chloride (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v)
A7	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium di-hydrogen phosphate 0.2 M - 🔍	pH 8.5	66 μl of Ammonium di-hydrogen phosphate (3 M) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 333 μl of water (100 v/v)
A8	Polyethylene glycol 3,350 20 w/v - Potassium nitrate 0.2 M - 🔍	NA	200 μl of Potassium nitrate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v)
B1	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 266 μl of Ammonium sulfate (3 M) 633 μl of water (100 v/v)
B2	Polyethylene glycol 3,350 20 w/v - Sodium thiocyanate 0.2 M - 🔍	NA	25 μl of Sodium thiocyanate (8 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 575 μl of water (100 v/v)
B3	Polyethylene glycol 6,000 20 w/v - BICINE 0.1 M pH 9 🔍	pH 9	400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 500 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 10 w/v - HEPES 0.1 M pH 7.5 Ethylene glycol 8 v/v - 🔍	pH 7.5	80 μl of Ethylene glycol (100 v/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 8 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v)
B6	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 500 μl of water (100 v/v)
B7	tri-Sodium citrate 1.6 M - 🔍	NA	800 μl of tri-Sodium citrate (2 M) 200 μl of water (100 v/v)
B8	Polyethylene glycol 3,350 20 w/v - tri-Potassium citrate 0.2 M - 🔍	NA	80 μl of tri-Potassium citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 20 w/v - tri-Sodium citrate 0.1 M pH 5.5 2-Propanol 10 v/v - 🔍	pH 5.5	100 μl of 2-Propanol (100 v/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 400 μl of water (100 v/v)
C2	Polyethylene glycol 6,000 20 w/v - tri-Sodium citrate 0.1 M pH 4 Lithium chloride 1 M - 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 200 μl of Lithium chloride (5 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 300 μl of water (100 v/v)
C3	Polyethylene glycol 3,350 20 w/v - Ammonium nitrate 0.2 M - 🔍	NA	20 μl of Ammonium nitrate (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v)
C4	Polyethylene glycol 6,000 10 w/v - HEPES 0.1 M pH 7 🔍	pH 7	200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 700 μl of water (100 v/v)
C5	Sodium di-hydrogen phosphate 0.8 M - HEPES 0.1 M pH 7.5 di-Potassium hydrogen phosphate 0.8 M - 🔍	pH 7.5	200 μl of Sodium di-hydrogen phosphate (4 M) 100 μl of HEPES pH 7.5 (1 M) 160 μl of di-Potassium hydrogen phosphate (5 M) 540 μl of water (100 v/v)
C6	Ethanol 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400 μl of Ethanol (50 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of water (100 v/v)
C7	Polyethylene glycol 20,000 10 w/v - BICINE 0.1 M pH 9 1,4-Dioxane 2 v/v - 🔍	pH 9	200 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 40 μl of 1,4-Dioxane (50 v/v) 660 μl of water (100 v/v)
C8	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	666 μl of Ammonium sulfate (3 M) 100 μl of Sodium acetate pH 4.6 (1 M) 233 μl of water (100 v/v)
D1	Polyethylene glycol 1,000 10 w/v - Polyethylene glycol 8,000 10 w/v - 🔍	NA	200 μl of Polyethylene glycol 1,000 (50 w/v) 200 μl of Polyethylene glycol 8,000 (50 w/v) 600 μl of water (100 v/v)
D2	Polyethylene glycol 1,500 24 w/v - Glycerol 20 v/v - 🔍	NA	200 μl of Glycerol (100 v/v) 480 μl of Polyethylene glycol 1,500 (50 w/v) 320 μl of water (100 v/v)
D3	Polyethylene glycol 400 30 v/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.2 M - 🔍	pH 7.5	80 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 520 μl of water (100 v/v)
D4	2-methyl-2,4-pentanediol BCC 70 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v)
D5	2-methyl-2,4-pentanediol BCC 40 v/v - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 25.5 w/v - Ammonium sulfate 0.17 M - Glycerol 15 v/v - 🔍	NA	150 μl of Glycerol (100 v/v) 56 μl of Ammonium sulfate (3 M) 510 μl of Polyethylene glycol 4,000 (50 w/v) 283 μl of water (100 v/v)
D7	2-Propanol 14 v/v - Sodium acetate 0.07 M pH 4.6 Calcium chloride 0.14 M - Glycerol 30 v/v - 🔍	pH 4.6	140 μl of 2-Propanol (100 v/v) 300 μl of Glycerol (100 v/v) 70 μl of Sodium acetate pH 4.6 (1 M) 140 μl of Calcium chloride (1 M) 350 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 16 w/v - Potassium di-hydrogen phosphate 0.04 M - Glycerol 20 v/v - 🔍	NA	200 μl of Glycerol (100 v/v) 20 μl of Potassium di-hydrogen phosphate (2 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v)
E1	Magnesium sulfate 1.6 M - MES 0.1 M pH 6.5 🔍	pH 6.5	33 μl of MES pH 6.5 (3 M) 800 μl of Magnesium sulfate (2 M) 166 μl of water (100 v/v)
E2	Polyethylene glycol 6,000 10 w/v - BICINE 0.1 M pH 9 🔍	pH 9	200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 700 μl of water (100 v/v)
E3	Polyethylene glycol 8,000 14.4 w/v - Sodium cacodylate 0.08 M pH 6.5 Calcium acetate 0.16 M - Glycerol 20 v/v - 🔍	pH 6.5	200 μl of Glycerol (100 v/v) 160 μl of Calcium acetate (1 M) 288 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Sodium cacodylate pH 6.5 (1 M) 272 μl of water (100 v/v)
E4	Jeffamine® M-600 30 v/v - MES 0.1 M pH 6.5 Cesium chloride 0.05 M - 🔍	pH 6.5	33 μl of MES pH 6.5 (3 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 50 μl of Cesium chloride (1 M) 316 μl of water (100 v/v)
E5	Ammonium sulfate 3.2 M - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	800 μl of Ammonium sulfate (4 M) 100 μl of tri-Sodium citrate pH 5 (1 M) 100 μl of water (100 v/v)
E6	Polyethylene glycol 10,000 15 w/v - tri-Sodium citrate 0.1 M pH 5.5 1,4-Dioxane 2 v/v - 🔍	pH 5.5	100 μl of tri-Sodium citrate pH 5.5 (1 M) 300 μl of Polyethylene glycol 10,000 (50 w/v) 40 μl of 1,4-Dioxane (50 v/v) 560 μl of water (100 v/v)
E7	Jeffamine® M-600 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	400 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)
E8	2-methyl-2,4-pentanediol BCC 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BICINE pH 9 (1 M) 800 μl of water (100 v/v)
F1	Polyethylene glycol 400 28 v/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.2 M - 🔍	pH 7.5	280 μl of Polyethylene glycol 400 (100 v/v) 200 μl of Calcium chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 420 μl of water (100 v/v)
F2	Polyethylene glycol 4,000 30 w/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 220 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 30 w/v - Ammonium sulfate 0.2 M - 🔍	NA	66 μl of Ammonium sulfate (3 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 333 μl of water (100 v/v)
F4	Polyethylene glycol monomethyl ether 5,000 30 w/v - tris chloride BCC 0.1 M pH 8 Lithium sulfate 0.2 M - 🔍	pH 8	80 μl of Lithium sulfate (2.5 M) 100 μl of tris chloride BCC pH 8 (1 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 220 μl of water (100 v/v)
F5	Ammonium sulfate 1.5 M - tris chloride BCC 0.1 M pH 8.5 Glycerol 12 v/v - 🔍	pH 8.5	120 μl of Glycerol (100 v/v) 500 μl of Ammonium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 280 μl of water (100 v/v)
F6	2-methyl-2,4-pentanediol BCC 50 v/v - tris chloride BCC 0.1 M pH 8.5 Ammonium chloride 0.2 M - 🔍	pH 8.5	500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 40 μl of Ammonium chloride (5 M) 360 μl of water (100 v/v)
F7	Polyethylene glycol monomethyl ether 5,000 30 w/v - MES 0.1 M pH 6.5 Ammonium sulfate 0.2 M - 🔍	pH 6.5	33 μl of MES pH 6.5 (3 M) 66 μl of Ammonium sulfate (3 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 300 μl of water (100 v/v)
F8	Polyethylene glycol 10,000 20 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	400 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)