C6 - Screen Contents

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Supplemental Information

Screen Description

Wizard IV

Wizard / Wizard IV

Created 2013-01-04.

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Wizard IV

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Wizard IV

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Screen Structure

Wizard IV

Well Count	48
Number of distinct ingredients	53
Internal diversity (score mean dist)	0.961

Screen Composition

More information about screen composition chart.

ingredient type	Wizard IV
Buffer	54
Cryoprotectant	1
Organic (non-volatile)	15
Organic (volatile)	6
Polymer	40
Salt	48

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard IV Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - di-Potassium hydrogen phosphate 0.04 M - Polyethylene glycol 8,000 16 w/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 8.0 μl of di-Potassium hydrogen phosphate (5 M) 472.0 μl of water (100 v/v)
A2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 8 Sodium chloride 0.1 M - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 8	50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 300.0 μl of Ethanol (50 v/v) 20.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 530.0 μl of water (100 v/v)
A3	Ethanol 40 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 1,000 5 w/v - 🔍	pH 4.2	800.0 μl of Ethanol (50 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
A4	Ammonium sulfate 0.2 M - BIS-TRIS 0.1 M pH 5.5 🔍	pH 5.5	66.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 833.3 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Polyethylene glycol 400 2 v/v - 🔍	pH 5.5	666.7 μl of Ammonium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 280.0 μl of water (100 v/v)
A6	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	266.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 633.3 μl of water (100 v/v)
A7	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 4.5 Magnesium sulfate 0.1 M - 2-Propanol 5 v/v - 🔍	pH 4.5	50.0 μl of 2-Propanol (100 v/v) 666.7 μl of Lithium sulfate (3 M) 100.0 μl of Magnesium sulfate (1 M) 33.3 μl of Sodium acetate pH 4.5 (3 M) 150.0 μl of water (100 v/v)
A8	Lithium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 2 v/v - 🔍	pH 8.5	666.7 μl of Lithium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 213.3 μl of water (100 v/v)
B1	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Magnesium sulfate 0.1 M - Polyethylene glycol 400 5 v/v - 🔍	pH 5.5	666.7 μl of Lithium sulfate (3 M) 100.0 μl of Magnesium sulfate (1 M) 50.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 150.0 μl of water (100 v/v)
B2	Polyethylene glycol 200 50 v/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 320.0 μl of water (100 v/v)
B3	Polyethylene glycol 300 40 v/v - Sodium cacodylate 0.1 M pH 6.5 Calcium acetate 0.2 M - 🔍	pH 6.5	200.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 300.0 μl of water (100 v/v)
B4	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 300.0 μl of water (100 v/v)
B5	di-Sodium succinate 0.8 M - 🔍	NA	266.7 μl of di-Sodium succinate pH 7 (3 M) 733.3 μl of water (100 v/v)
B6	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	200.0 μl of Lithium sulfate (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
B7	Polyethylene glycol 400 50 v/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	200.0 μl of Lithium sulfate (1 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 266.7 μl of water (100 v/v)
B8	Polyethylene glycol monomethyl ether 550 15 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	33.3 μl of MES pH 6.5 (3 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 666.7 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 5.5 🔍	pH 5.5	500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 5.5 (1 M) 400.0 μl of water (100 v/v)
C2	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 8.5 🔍	pH 8.5	500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C3	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MMT buffer pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C4	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 9 🔍	pH 9	100.0 μl of MMT buffer pH 9 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 25 w/v - MIB buffer 0.1 M pH 5 🔍	pH 5	100.0 μl of MIB buffer pH 5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C6	Polyethylene glycol 1,500 25 w/v - PCB buffer 0.1 M pH 7 🔍	pH 7	100.0 μl of PCB buffer pH 7 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v)
C7	Polyethylene glycol 1,500 12 w/v - Sodium acetate 0.1 M pH 5.5 Sodium chloride 2.5 M - 2-methyl-2,4-pentanediol BCC 1.5 v/v - 🔍	pH 5.5	15.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 240.0 μl of Polyethylene glycol 1,500 (50 w/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 500.0 μl of Sodium chloride (5 M) 211.7 μl of water (100 v/v)
C8	di-Sodium malonate 2.4 M - 🔍	NA	800.0 μl of di-Sodium malonate (3 M) 200.0 μl of water (100 v/v)
D1	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium bromide 0.15 M - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v)
D2	Polyethylene glycol monomethyl ether 2,000 10 w/v - Sodium acetate 0.1 M pH 5.5 Ammonium sulfate 0.2 M - 🔍	pH 5.5	66.7 μl of Ammonium sulfate (3 M) 200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 700.0 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Trimethylamine N-oxide 0.2 M - 🔍	pH 8.5	400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
D4	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 6.5 Sodium fluoride 0.2 M - 🔍	pH 6.5	100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 250.0 μl of water (100 v/v)
D5	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.1 M pH 4 tri-Sodium citrate 0.2 M - 🔍	pH 4	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 400.0 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 8.5 di-Sodium malonate 0.2 M - 🔍	pH 8.5	100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate (3 M) 433.3 μl of water (100 v/v)
D7	Poly(acrylic acid sodium salt) 5,100 20 w/v - HEPES 0.1 M pH 7 Magnesium chloride 0.02 M - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 492.0 μl of water (100 v/v)
D8	di-Sodium DL-malate 2.1 M - 🔍	NA	700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v)
E1	di-Potassium hydrogen phosphate 0.8 M - HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 0.8 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Sodium di-hydrogen phosphate (4 M) 160.0 μl of di-Potassium hydrogen phosphate (5 M) 540.0 μl of water (100 v/v)
E2	Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6 Ammonium chloride 0.2 M - 🔍	pH 6	40.0 μl of Ammonium chloride (5 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 460.0 μl of water (100 v/v)
E3	Polyethylene glycol 6,000 20 w/v - HEPES 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 460.0 μl of water (100 v/v)
E4	Polyethylene glycol 6,000 20 w/v - tris chloride BCC 0.1 M pH 8 Lithium chloride 0.2 M - 🔍	pH 8	200.0 μl of Lithium chloride (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v)
E5	Polyvinylpyrrolidone K15 20 w/v - tris chloride BCC 0.1 M pH 8.5 Cobalt (II) chloride 0.1 M - 🔍	pH 8.5	100.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
E6	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 20 w/v - Imidazole 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 3 v/v - 🔍	pH 6.5	30.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 470.0 μl of water (100 v/v)
E8	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.1 M - Polyethylene glycol 400 20 v/v - 🔍	pH 8.5	40.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 260.0 μl of water (100 v/v)
F1	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 0.2 M - 2-Propanol 10 v/v - 🔍	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 333.3 μl of water (100 v/v)
F2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 1,500 25 w/v - 🔍	pH 4.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 166.7 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 30 v/v - Imidazole 0.1 M pH 6.5 Ammonium sulfate 0.2 M - Polyethylene glycol 3,350 10 w/v - 🔍	pH 6.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of Imidazole pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v)
F4	2-methyl-2,4-pentanediol BCC 30 v/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.5 M - Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 340.0 μl of water (100 v/v)
F5	2-Propanol 40 v/v - Imidazole 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	400.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of water (100 v/v)
F6	2-Propanol 30 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 30 w/v - 🔍	pH 8.5	300.0 μl of 2-Propanol (100 v/v) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M)
F7	Polyethylene glycol 10,000 17 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 0.1 M - 🔍	pH 5.5	20.0 μl of Ammonium acetate (5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 540.0 μl of water (100 v/v)
F8	Polyethylene glycol 20,000 15 w/v - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of HEPES pH 7 (1 M) 300.0 μl of Polyethylene glycol 20,000 (50 w/v) 600.0 μl of water (100 v/v)