C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

Wizard IV

Wizard / Wizard IV

Created 2013-01-04.

Export

Wizard IV

Barcodes

Wizard IV

Screen Structure

Wizard IV

Well Count	48
Number of distinct ingredients	53
Internal diversity (score mean dist)	0.961

Screen Composition

More information about screen composition chart.

ingredient type	Wizard IV
Buffer	54
Cryoprotectant	1
Organic (non-volatile)	15
Organic (volatile)	6
Polymer	40
Salt	48

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Wizard IV Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Glycerol 20 v/v - di-Potassium hydrogen phosphate 0.04 M - Polyethylene glycol 8,000 16 w/v - 🔍	NA	320 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 8 μl of di-Potassium hydrogen phosphate (5 M) 472 μl of water (100 v/v)
A2	Ethanol 15 v/v - tris chloride BCC 0.1 M pH 8 Sodium chloride 0.1 M - 2-methyl-2,4-pentanediol BCC 5 v/v - 🔍	pH 8	300 μl of Ethanol (50 v/v) 20 μl of Sodium chloride (5 M) 100 μl of tris chloride BCC pH 8 (1 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 530 μl of water (100 v/v)
A3	Ethanol 40 v/v - Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 1,000 5 w/v - 🔍	pH 4.2	800 μl of Ethanol (50 v/v) 100 μl of Polyethylene glycol 1,000 (50 w/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M)
A4	Ammonium sulfate 0.2 M - BIS-TRIS 0.1 M pH 5.5 🔍	pH 5.5	100 μl of BIS-TRIS pH 5.5 (1 M) 66 μl of Ammonium sulfate (3 M) 833 μl of water (100 v/v)
A5	Ammonium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Polyethylene glycol 400 2 v/v - 🔍	pH 5.5	20 μl of Polyethylene glycol 400 (100 v/v) 33 μl of Sodium acetate pH 5.5 (3 M) 666 μl of Ammonium sulfate (3 M) 280 μl of water (100 v/v)
A6	Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 4 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 266 μl of Ammonium sulfate (3 M) 633 μl of water (100 v/v)
A7	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 4.5 Magnesium sulfate 0.1 M - 2-Propanol 5 v/v - 🔍	pH 4.5	666 μl of Lithium sulfate (3 M) 33 μl of Sodium acetate pH 4.5 (3 M) 100 μl of Magnesium sulfate (1 M) 50 μl of 2-Propanol (100 v/v) 150 μl of water (100 v/v)
A8	Lithium sulfate 2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 400 2 v/v - 🔍	pH 8.5	666 μl of Lithium sulfate (3 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 20 μl of Polyethylene glycol 400 (100 v/v) 213 μl of water (100 v/v)
B1	Lithium sulfate 2 M - Sodium acetate 0.1 M pH 5.5 Magnesium sulfate 0.1 M - Polyethylene glycol 400 5 v/v - 🔍	pH 5.5	666 μl of Lithium sulfate (3 M) 100 μl of Magnesium sulfate (1 M) 50 μl of Polyethylene glycol 400 (100 v/v) 33 μl of Sodium acetate pH 5.5 (3 M) 150 μl of water (100 v/v)
B2	Polyethylene glycol 200 50 v/v - Sodium cacodylate 0.1 M pH 6.5 Magnesium chloride 0.2 M - 🔍	pH 6.5	100 μl of Sodium cacodylate pH 6.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 500 μl of Polyethylene glycol 200 (100 v/v) 320 μl of water (100 v/v)
B3	Polyethylene glycol 300 40 v/v - Sodium cacodylate 0.1 M pH 6.5 Calcium acetate 0.2 M - 🔍	pH 6.5	200 μl of Calcium acetate (1 M) 100 μl of Sodium cacodylate pH 6.5 (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 300 μl of water (100 v/v)
B4	Jeffamine® M-600 30 v/v - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of HEPES pH 7 (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 300 μl of water (100 v/v)
B5	di-Sodium succinate 0.8 M - 🔍	NA	266 μl of di-Sodium succinate pH 7 (3 M) 733 μl of water (100 v/v)
B6	Polyethylene glycol 400 40 v/v - tris chloride BCC 0.1 M pH 8.5 Lithium sulfate 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 200 μl of Lithium sulfate (1 M) 300 μl of water (100 v/v)
B7	Polyethylene glycol 400 50 v/v - Sodium acetate 0.1 M pH 4.5 Lithium sulfate 0.2 M - 🔍	pH 4.5	33 μl of Sodium acetate pH 4.5 (3 M) 500 μl of Polyethylene glycol 400 (100 v/v) 200 μl of Lithium sulfate (1 M) 266 μl of water (100 v/v)
B8	Polyethylene glycol monomethyl ether 550 15 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	300 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 33 μl of MES pH 6.5 (3 M) 666 μl of water (100 v/v)
C1	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 5.5 🔍	pH 5.5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of SPG buffer pH 5.5 (1 M) 400 μl of water (100 v/v)
C2	Polyethylene glycol 1,500 25 w/v - SPG buffer 0.1 M pH 8.5 🔍	pH 8.5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of SPG buffer pH 8.5 (1 M) 400 μl of water (100 v/v)
C3	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 6.5 🔍	pH 6.5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MMT buffer pH 6.5 (1 M) 400 μl of water (100 v/v)
C4	Polyethylene glycol 1,500 25 w/v - MMT buffer 0.1 M pH 9 🔍	pH 9	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MMT buffer pH 9 (1 M) 400 μl of water (100 v/v)
C5	Polyethylene glycol 1,500 25 w/v - MIB buffer 0.1 M pH 5 🔍	pH 5	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MIB buffer pH 5 (1 M) 400 μl of water (100 v/v)
C6	Polyethylene glycol 1,500 25 w/v - PCB buffer 0.1 M pH 7 🔍	pH 7	500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of PCB buffer pH 7 (1 M) 400 μl of water (100 v/v)
C7	Polyethylene glycol 1,500 12 w/v - Sodium acetate 0.1 M pH 5.5 Sodium chloride 2.5 M - 2-methyl-2,4-pentanediol BCC 1.5 v/v - 🔍	pH 5.5	500 μl of Sodium chloride (5 M) 240 μl of Polyethylene glycol 1,500 (50 w/v) 33 μl of Sodium acetate pH 5.5 (3 M) 15 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 211 μl of water (100 v/v)
C8	di-Sodium malonate 2.4 M - 🔍	NA	800 μl of di-Sodium malonate (3 M) 200 μl of water (100 v/v)
D1	Polyethylene glycol monomethyl ether 2,000 30 w/v - Potassium bromide 0.15 M - 🔍	NA	37 μl of Potassium bromide (4 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 362 μl of water (100 v/v)
D2	Polyethylene glycol monomethyl ether 2,000 10 w/v - Sodium acetate 0.1 M pH 5.5 Ammonium sulfate 0.2 M - 🔍	pH 5.5	200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 33 μl of Sodium acetate pH 5.5 (3 M) 66 μl of Ammonium sulfate (3 M) 700 μl of water (100 v/v)
D3	Polyethylene glycol monomethyl ether 2,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Trimethylamine N-oxide 0.2 M - 🔍	pH 8.5	400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200 μl of Trimethylamine N-oxide (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of water (100 v/v)
D4	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 6.5 Sodium fluoride 0.2 M - 🔍	pH 6.5	250 μl of Sodium fluoride (0.8 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 250 μl of water (100 v/v)
D5	Polyethylene glycol 3,350 20 w/v - tri-Sodium citrate 0.1 M pH 4 tri-Sodium citrate 0.2 M - 🔍	pH 4	100 μl of tri-Sodium citrate pH 4 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate (2 M) 400 μl of water (100 v/v)
D6	Polyethylene glycol 3,350 20 w/v - BIS-TRIS propane 0.1 M pH 8.5 di-Sodium malonate 0.2 M - 🔍	pH 8.5	400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of di-Sodium malonate (3 M) 100 μl of BIS-TRIS propane pH 8.5 (1 M) 433 μl of water (100 v/v)
D7	Poly(acrylic acid sodium salt) 5,100 20 w/v - HEPES 0.1 M pH 7 Magnesium chloride 0.02 M - 🔍	pH 7	400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 100 μl of HEPES pH 7 (1 M) 8 μl of Magnesium chloride (2.5 M) 492 μl of water (100 v/v)
D8	di-Sodium DL-malate 2.1 M - 🔍	NA	700 μl of di-Sodium DL-malate pH 7 (3 M) 300 μl of water (100 v/v)
E1	di-Potassium hydrogen phosphate 0.8 M - HEPES 0.1 M pH 7.5 Sodium di-hydrogen phosphate 0.8 M - 🔍	pH 7.5	200 μl of Sodium di-hydrogen phosphate (4 M) 160 μl of di-Potassium hydrogen phosphate (5 M) 100 μl of HEPES pH 7.5 (1 M) 540 μl of water (100 v/v)
E2	Polyethylene glycol 6,000 20 w/v - MES 0.1 M pH 6 Ammonium chloride 0.2 M - 🔍	pH 6	40 μl of Ammonium chloride (5 M) 100 μl of MES pH 6 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 460 μl of water (100 v/v)
E3	Polyethylene glycol 6,000 20 w/v - HEPES 0.1 M pH 7 Sodium chloride 0.2 M - 🔍	pH 7	40 μl of Sodium chloride (5 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 460 μl of water (100 v/v)
E4	Polyethylene glycol 6,000 20 w/v - tris chloride BCC 0.1 M pH 8 Lithium chloride 0.2 M - 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 200 μl of Lithium chloride (1 M) 300 μl of water (100 v/v)
E5	Polyvinylpyrrolidone K15 20 w/v - tris chloride BCC 0.1 M pH 8.5 Cobalt (II) chloride 0.1 M - 🔍	pH 8.5	100 μl of Cobalt (II) chloride (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 400 μl of water (100 v/v)
E6	Ethylene glycol 50 v/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.2 M - 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Ethylene glycol (100 v/v) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v)
E7	Polyethylene glycol 8,000 20 w/v - Imidazole 0.1 M pH 6.5 2-methyl-2,4-pentanediol BCC 3 v/v - 🔍	pH 6.5	100 μl of Imidazole pH 6.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 30 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 470 μl of water (100 v/v)
E8	Polyethylene glycol 8,000 20 w/v - tris chloride BCC 0.1 M pH 8.5 Magnesium chloride 0.1 M - Polyethylene glycol 400 20 v/v - 🔍	pH 8.5	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Polyethylene glycol 400 (100 v/v) 40 μl of Magnesium chloride (2.5 M) 260 μl of water (100 v/v)
F1	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Ammonium sulfate 0.2 M - 2-Propanol 10 v/v - 🔍	pH 7.5	400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 66 μl of Ammonium sulfate (3 M) 100 μl of 2-Propanol (100 v/v) 333 μl of water (100 v/v)
F2	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium acetate 0.1 M pH 4.5 Polyethylene glycol 1,500 25 w/v - 🔍	pH 4.5	500 μl of Polyethylene glycol 1,500 (50 w/v) 33 μl of Sodium acetate pH 4.5 (3 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 166 μl of water (100 v/v)
F3	2-methyl-2,4-pentanediol BCC 30 v/v - Imidazole 0.1 M pH 6.5 Ammonium sulfate 0.2 M - Polyethylene glycol 3,350 10 w/v - 🔍	pH 6.5	200 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Imidazole pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66 μl of Ammonium sulfate (3 M) 333 μl of water (100 v/v)
F4	2-methyl-2,4-pentanediol BCC 30 v/v - tris chloride BCC 0.1 M pH 8.5 Sodium chloride 0.5 M - Polyethylene glycol 8,000 8 w/v - 🔍	pH 8.5	100 μl of Sodium chloride (5 M) 160 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 340 μl of water (100 v/v)
F5	2-Propanol 40 v/v - Imidazole 0.1 M pH 6.5 Polyethylene glycol 8,000 15 w/v - 🔍	pH 6.5	100 μl of Imidazole pH 6.5 (1 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 400 μl of 2-Propanol (100 v/v) 200 μl of water (100 v/v)
F6	2-Propanol 30 v/v - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 30 w/v - 🔍	pH 8.5	600 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of 2-Propanol (100 v/v)
F7	Polyethylene glycol 10,000 17 w/v - BIS-TRIS 0.1 M pH 5.5 Ammonium acetate 0.1 M - 🔍	pH 5.5	340 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 20 μl of Ammonium acetate (5 M) 540 μl of water (100 v/v)
F8	Polyethylene glycol 20,000 15 w/v - HEPES 0.1 M pH 7 🔍	pH 7	300 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 600 μl of water (100 v/v)