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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Wizard III&IV
Barcodes
Wizard III&IV
Screen Structure
Wizard III&IV
Well Count | 96 |
---|---|
Number of distinct ingredients | 69 |
Internal diversity (score mean dist) | 0.961 |
Screen Composition
ingredient type | Wizard III&IV |
---|---|
Buffer | 99 |
Cryoprotectant | 7 |
Organic (non-volatile) | 32 |
Organic (volatile) | 11 |
Polymer | 74 |
Salt | 91 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 80 μl of di-Ammonium hydrogen citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v) |
A2 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 20 μl of Calcium chloride (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 580 μl of water (100 v/v) |
A3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium formate (1 M) 400 μl of water (100 v/v) |
A4 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium formate (5 M) 560 μl of water (100 v/v) |
A5 |
| NA | 40 μl of Ammonium chloride (5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 560 μl of water (100 v/v) |
A6 |
| NA | 200 μl of Barium chloride (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
A7 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 66 μl of Ammonium di-hydrogen phosphate (3 M) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 333 μl of water (100 v/v) |
A8 |
| NA | 200 μl of Potassium nitrate (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 400 μl of water (100 v/v) |
A9 |
| pH 4 | 266 μl of Ammonium sulfate (3 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 633 μl of water (100 v/v) |
A10 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 25 μl of Sodium thiocyanate (8 M) 575 μl of water (100 v/v) |
A11 |
| pH 9 | 100 μl of BICINE pH 9 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 500 μl of water (100 v/v) |
A12 |
| pH 7.5 | 80 μl of Ethylene glycol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 620 μl of water (100 v/v) |
B1 |
| pH 4.6 | 100 μl of Sodium acetate pH 4.6 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v) |
B2 |
| pH 5 | 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 500 μl of water (100 v/v) |
B3 |
| NA | 800 μl of tri-Sodium citrate (2 M) 200 μl of water (100 v/v) |
B4 |
| NA | 80 μl of tri-Potassium citrate (2.5 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 520 μl of water (100 v/v) |
B5 |
| pH 5.5 | 100 μl of 2-Propanol (100 v/v) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 400 μl of water (100 v/v) |
B6 |
| pH 4 | 200 μl of Lithium chloride (5 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of tri-Sodium citrate pH 4 (1 M) 300 μl of water (100 v/v) |
B7 |
| NA | 20 μl of Ammonium nitrate (10 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 580 μl of water (100 v/v) |
B8 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
B9 |
| pH 7.5 | 160 μl of di-Potassium hydrogen phosphate (5 M) 200 μl of Sodium di-hydrogen phosphate (4 M) 100 μl of HEPES pH 7.5 (1 M) 540 μl of water (100 v/v) |
B10 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Ethanol (50 v/v) 500 μl of water (100 v/v) |
B11 |
| pH 9 | 40 μl of 1,4-Dioxane (50 v/v) 200 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of BICINE pH 9 (1 M) 660 μl of water (100 v/v) |
B12 |
| pH 4.6 | 666 μl of Ammonium sulfate (3 M) 100 μl of Sodium acetate pH 4.6 (1 M) 233 μl of water (100 v/v) |
C1 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v) |
C2 |
| NA | 480 μl of Polyethylene glycol 1,500 (50 w/v) 200 μl of Glycerol (100 v/v) 320 μl of water (100 v/v) |
C3 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 300 μl of Polyethylene glycol 400 (100 v/v) 520 μl of water (100 v/v) |
C4 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of water (100 v/v) |
C5 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500 μl of water (100 v/v) |
C6 |
| NA | 150 μl of Glycerol (100 v/v) 56 μl of Ammonium sulfate (3 M) 510 μl of Polyethylene glycol 4,000 (50 w/v) 283 μl of water (100 v/v) |
C7 |
| pH 4.6 | 300 μl of Glycerol (100 v/v) 70 μl of Sodium acetate pH 4.6 (1 M) 140 μl of 2-Propanol (100 v/v) 140 μl of Calcium chloride (1 M) 350 μl of water (100 v/v) |
C8 |
| NA | 200 μl of Glycerol (100 v/v) 20 μl of Potassium di-hydrogen phosphate (2 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v) |
C9 |
| pH 6.5 | 33 μl of MES pH 6.5 (3 M) 800 μl of Magnesium sulfate (2 M) 166 μl of water (100 v/v) |
C10 |
| pH 9 | 100 μl of BICINE pH 9 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 700 μl of water (100 v/v) |
C11 |
| pH 6.5 | 200 μl of Glycerol (100 v/v) 288 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Sodium cacodylate pH 6.5 (1 M) 160 μl of Calcium acetate (1 M) 272 μl of water (100 v/v) |
C12 |
| pH 6.5 | 50 μl of Cesium chloride (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 33 μl of MES pH 6.5 (3 M) 316 μl of water (100 v/v) |
D1 |
| pH 5 | 800 μl of Ammonium sulfate (4 M) 100 μl of tri-Sodium citrate pH 5 (1 M) 100 μl of water (100 v/v) |
D2 |
| pH 5.5 | 40 μl of 1,4-Dioxane (50 v/v) 300 μl of Polyethylene glycol 10,000 (50 w/v) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 560 μl of water (100 v/v) |
D3 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 500 μl of water (100 v/v) |
D4 |
| pH 9 | 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BICINE pH 9 (1 M) 800 μl of water (100 v/v) |
D5 |
| pH 7.5 | 200 μl of Calcium chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 280 μl of Polyethylene glycol 400 (100 v/v) 420 μl of water (100 v/v) |
D6 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of Polyethylene glycol 4,000 (50 w/v) 80 μl of Lithium sulfate (2.5 M) 220 μl of water (100 v/v) |
D7 |
| NA | 66 μl of Ammonium sulfate (3 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 333 μl of water (100 v/v) |
D8 |
| pH 8 | 100 μl of tris chloride BCC pH 8 (1 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 80 μl of Lithium sulfate (2.5 M) 220 μl of water (100 v/v) |
D9 |
| pH 8.5 | 120 μl of Glycerol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Ammonium sulfate (3 M) 280 μl of water (100 v/v) |
D10 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 40 μl of Ammonium chloride (5 M) 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 360 μl of water (100 v/v) |
D11 |
| pH 6.5 | 66 μl of Ammonium sulfate (3 M) 600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 33 μl of MES pH 6.5 (3 M) 300 μl of water (100 v/v) |
D12 |
| pH 7.5 | 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 10,000 (50 w/v) 500 μl of water (100 v/v) |
E1 |
| NA | 200 μl of Glycerol (100 v/v) 8 μl of di-Potassium hydrogen phosphate (5 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 472 μl of water (100 v/v) |
E2 |
| pH 8 | 300 μl of Ethanol (50 v/v) 100 μl of tris chloride BCC pH 8 (1 M) 20 μl of Sodium chloride (5 M) 50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 530 μl of water (100 v/v) |
E3 |
| pH 4.2 | 800 μl of Ethanol (50 v/v) 100 μl of Sodium phosphate citrate pH 4.2 (1 M) 100 μl of Polyethylene glycol 1,000 (50 w/v) |
E4 |
| pH 5.5 | 66 μl of Ammonium sulfate (3 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 833 μl of water (100 v/v) |
E5 |
| pH 5.5 | 33 μl of Sodium acetate pH 5.5 (3 M) 666 μl of Ammonium sulfate (3 M) 20 μl of Polyethylene glycol 400 (100 v/v) 280 μl of water (100 v/v) |
E6 |
| pH 4 | 266 μl of Ammonium sulfate (3 M) 100 μl of tri-Sodium citrate pH 4 (1 M) 633 μl of water (100 v/v) |
E7 |
| pH 4.5 | 33 μl of Sodium acetate pH 4.5 (3 M) 50 μl of 2-Propanol (100 v/v) 666 μl of Lithium sulfate (3 M) 100 μl of Magnesium sulfate (1 M) 150 μl of water (100 v/v) |
E8 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 666 μl of Lithium sulfate (3 M) 20 μl of Polyethylene glycol 400 (100 v/v) 213 μl of water (100 v/v) |
E9 |
| pH 5.5 | 33 μl of Sodium acetate pH 5.5 (3 M) 666 μl of Lithium sulfate (3 M) 100 μl of Magnesium sulfate (1 M) 50 μl of Polyethylene glycol 400 (100 v/v) 150 μl of water (100 v/v) |
E10 |
| pH 6.5 | 500 μl of Polyethylene glycol 200 (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
E11 |
| pH 6.5 | 400 μl of Polyethylene glycol 300 (100 v/v) 100 μl of Sodium cacodylate pH 6.5 (1 M) 200 μl of Calcium acetate (1 M) 300 μl of water (100 v/v) |
E12 |
| pH 7 | 100 μl of HEPES pH 7 (1 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 300 μl of water (100 v/v) |
F1 |
| NA | 266 μl of di-Sodium succinate pH 7 (3 M) 733 μl of water (100 v/v) |
F2 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 200 μl of Lithium sulfate (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 300 μl of water (100 v/v) |
F3 |
| pH 4.5 | 33 μl of Sodium acetate pH 4.5 (3 M) 200 μl of Lithium sulfate (1 M) 500 μl of Polyethylene glycol 400 (100 v/v) 266 μl of water (100 v/v) |
F4 |
| pH 6.5 | 300 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 33 μl of MES pH 6.5 (3 M) 666 μl of water (100 v/v) |
F5 |
| pH 5.5 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of SPG buffer pH 5.5 (1 M) 400 μl of water (100 v/v) |
F6 |
| pH 8.5 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of SPG buffer pH 8.5 (1 M) 400 μl of water (100 v/v) |
F7 |
| pH 6.5 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MMT buffer pH 6.5 (1 M) 400 μl of water (100 v/v) |
F8 |
| pH 9 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MMT buffer pH 9 (1 M) 400 μl of water (100 v/v) |
F9 |
| pH 5 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of MIB buffer pH 5 (1 M) 400 μl of water (100 v/v) |
F10 |
| pH 7 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 100 μl of PCB buffer pH 7 (1 M) 400 μl of water (100 v/v) |
F11 |
| pH 5.5 | 240 μl of Polyethylene glycol 1,500 (50 w/v) 33 μl of Sodium acetate pH 5.5 (3 M) 500 μl of Sodium chloride (5 M) 15 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 211 μl of water (100 v/v) |
F12 |
| NA | 800 μl of di-Sodium malonate (3 M) 200 μl of water (100 v/v) |
G1 |
| NA | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37 μl of Potassium bromide (4 M) 362 μl of water (100 v/v) |
G2 |
| pH 5.5 | 33 μl of Sodium acetate pH 5.5 (3 M) 66 μl of Ammonium sulfate (3 M) 200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 700 μl of water (100 v/v) |
G3 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200 μl of Trimethylamine N-oxide (1 M) 300 μl of water (100 v/v) |
G4 |
| pH 6.5 | 250 μl of Sodium fluoride (0.8 M) 100 μl of BIS-TRIS propane pH 6.5 (1 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 250 μl of water (100 v/v) |
G5 |
| pH 4 | 100 μl of tri-Sodium citrate (2 M) 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of tri-Sodium citrate pH 4 (1 M) 400 μl of water (100 v/v) |
G6 |
| pH 8.5 | 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS propane pH 8.5 (1 M) 66 μl of di-Sodium malonate (3 M) 433 μl of water (100 v/v) |
G7 |
| pH 7 | 400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 100 μl of HEPES pH 7 (1 M) 8 μl of Magnesium chloride (2.5 M) 492 μl of water (100 v/v) |
G8 |
| NA | 700 μl of di-Sodium DL-malate pH 7 (3 M) 300 μl of water (100 v/v) |
G9 |
| pH 7.5 | 160 μl of di-Potassium hydrogen phosphate (5 M) 200 μl of Sodium di-hydrogen phosphate (4 M) 100 μl of HEPES pH 7.5 (1 M) 540 μl of water (100 v/v) |
G10 |
| pH 6 | 40 μl of Ammonium chloride (5 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of MES pH 6 (1 M) 460 μl of water (100 v/v) |
G11 |
| pH 7 | 40 μl of Sodium chloride (5 M) 100 μl of HEPES pH 7 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 460 μl of water (100 v/v) |
G12 |
| pH 8 | 200 μl of Lithium chloride (1 M) 100 μl of tris chloride BCC pH 8 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 300 μl of water (100 v/v) |
H1 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Cobalt (II) chloride (1 M) 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 400 μl of water (100 v/v) |
H2 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of Ethylene glycol (100 v/v) 80 μl of Magnesium chloride (2.5 M) 320 μl of water (100 v/v) |
H3 |
| pH 6.5 | 100 μl of Imidazole pH 6.5 (1 M) 30 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 400 μl of Polyethylene glycol 8,000 (50 w/v) 470 μl of water (100 v/v) |
H4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 40 μl of Magnesium chloride (2.5 M) 200 μl of Polyethylene glycol 400 (100 v/v) 260 μl of water (100 v/v) |
H5 |
| pH 7.5 | 66 μl of Ammonium sulfate (3 M) 100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 333 μl of water (100 v/v) |
H6 |
| pH 4.5 | 500 μl of Polyethylene glycol 1,500 (50 w/v) 33 μl of Sodium acetate pH 4.5 (3 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 166 μl of water (100 v/v) |
H7 |
| pH 6.5 | 66 μl of Ammonium sulfate (3 M) 200 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Imidazole pH 6.5 (1 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 333 μl of water (100 v/v) |
H8 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Sodium chloride (5 M) 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160 μl of Polyethylene glycol 8,000 (50 w/v) 340 μl of water (100 v/v) |
H9 |
| pH 6.5 | 400 μl of 2-Propanol (100 v/v) 100 μl of Imidazole pH 6.5 (1 M) 300 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of water (100 v/v) |
H10 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 300 μl of 2-Propanol (100 v/v) 600 μl of Polyethylene glycol 3,350 (50 w/v) |
H11 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 20 μl of Ammonium acetate (5 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 540 μl of water (100 v/v) |
H12 |
| pH 7 | 300 μl of Polyethylene glycol 20,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 600 μl of water (100 v/v) |