Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
Wizard III&IV
Barcodes
Wizard III&IV
No barcodes found.
Screen Structure
Wizard III&IV
Well Count | 96 |
---|---|
Number of distinct ingredients | 69 |
Internal diversity (score mean dist) | 0.961 |
Screen Composition
ingredient type | Wizard III&IV |
---|---|
Buffer | 99 |
Cryoprotectant | 7 |
Organic (non-volatile) | 32 |
Organic (volatile) | 11 |
Polymer | 74 |
Salt | 91 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of di-Ammonium hydrogen citrate (2.5 M) 520.0 μl of water (100 v/v) |
A2 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 20.0 μl of Calcium chloride (1 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 580.0 μl of water (100 v/v) |
A3 |
| NA | 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
A4 |
| NA | 40.0 μl of Ammonium formate (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v) |
A5 |
| NA | 40.0 μl of Ammonium chloride (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v) |
A6 |
| NA | 200.0 μl of Barium chloride (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
A7 |
| pH 8.5 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium di-hydrogen phosphate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v) |
A8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Potassium nitrate (1 M) 400.0 μl of water (100 v/v) |
A9 |
| pH 4 | 266.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 633.3 μl of water (100 v/v) |
A10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
A11 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
A12 |
| pH 7.5 | 80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v) |
B1 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
B2 |
| pH 5 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
B3 |
| NA | 800.0 μl of tri-Sodium citrate (2 M) 200.0 μl of water (100 v/v) |
B4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Potassium citrate (2.5 M) 520.0 μl of water (100 v/v) |
B5 |
| pH 5.5 | 100.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 400.0 μl of water (100 v/v) |
B6 |
| pH 4 | 200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 300.0 μl of water (100 v/v) |
B7 |
| NA | 20.0 μl of Ammonium nitrate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
B8 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
B9 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Sodium di-hydrogen phosphate (4 M) 160.0 μl of di-Potassium hydrogen phosphate (5 M) 540.0 μl of water (100 v/v) |
B10 |
| pH 8.5 | 400.0 μl of Ethanol (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
B11 |
| pH 9 | 40.0 μl of 1,4-Dioxane (50 v/v) 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 20,000 (50 w/v) 660.0 μl of water (100 v/v) |
B12 |
| pH 4.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v) |
C1 |
| NA | 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
C2 |
| NA | 200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v) |
C3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v) |
C4 |
| pH 7.5 | 700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v) |
C5 |
| pH 8 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 500.0 μl of water (100 v/v) |
C6 |
| NA | 56.7 μl of Ammonium sulfate (3 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 283.3 μl of water (100 v/v) |
C7 |
| pH 4.6 | 140.0 μl of 2-Propanol (100 v/v) 140.0 μl of Calcium chloride (1 M) 300.0 μl of Glycerol (100 v/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 350.0 μl of water (100 v/v) |
C8 |
| NA | 200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 20.0 μl of Potassium di-hydrogen phosphate (2 M) 460.0 μl of water (100 v/v) |
C9 |
| pH 6.5 | 33.3 μl of MES pH 6.5 (3 M) 800.0 μl of Magnesium sulfate (2 M) 166.7 μl of water (100 v/v) |
C10 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
C11 |
| pH 6.5 | 160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 272.0 μl of water (100 v/v) |
C12 |
| pH 6.5 | 50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 33.3 μl of MES pH 6.5 (3 M) 316.7 μl of water (100 v/v) |
D1 |
| pH 5 | 800.0 μl of Ammonium sulfate (4 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v) |
D2 |
| pH 5.5 | 40.0 μl of 1,4-Dioxane (50 v/v) 300.0 μl of Polyethylene glycol 10,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 560.0 μl of water (100 v/v) |
D3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 500.0 μl of water (100 v/v) |
D4 |
| pH 9 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 800.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 200.0 μl of Calcium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 420.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
D7 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 333.3 μl of water (100 v/v) |
D8 |
| pH 8 | 80.0 μl of Lithium sulfate (2.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 220.0 μl of water (100 v/v) |
D9 |
| pH 8.5 | 500.0 μl of Ammonium sulfate (3 M) 120.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 280.0 μl of water (100 v/v) |
D10 |
| pH 8.5 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 40.0 μl of Ammonium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 360.0 μl of water (100 v/v) |
D11 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 33.3 μl of MES pH 6.5 (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 300.0 μl of water (100 v/v) |
D12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v) |
E1 |
| NA | 200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 8.0 μl of di-Potassium hydrogen phosphate (5 M) 472.0 μl of water (100 v/v) |
E2 |
| pH 8 | 50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 300.0 μl of Ethanol (50 v/v) 20.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 530.0 μl of water (100 v/v) |
E3 |
| pH 4.2 | 800.0 μl of Ethanol (50 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) |
E4 |
| pH 5.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 833.3 μl of water (100 v/v) |
E5 |
| pH 5.5 | 666.7 μl of Ammonium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 280.0 μl of water (100 v/v) |
E6 |
| pH 4 | 266.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 633.3 μl of water (100 v/v) |
E7 |
| pH 4.5 | 50.0 μl of 2-Propanol (100 v/v) 666.7 μl of Lithium sulfate (3 M) 100.0 μl of Magnesium sulfate (1 M) 33.3 μl of Sodium acetate pH 4.5 (3 M) 150.0 μl of water (100 v/v) |
E8 |
| pH 8.5 | 666.7 μl of Lithium sulfate (3 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 213.3 μl of water (100 v/v) |
E9 |
| pH 5.5 | 666.7 μl of Lithium sulfate (3 M) 100.0 μl of Magnesium sulfate (1 M) 50.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 150.0 μl of water (100 v/v) |
E10 |
| pH 6.5 | 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 320.0 μl of water (100 v/v) |
E11 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 300.0 μl of water (100 v/v) |
E12 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 300.0 μl of water (100 v/v) |
F1 |
| NA | 266.7 μl of di-Sodium succinate pH 7 (3 M) 733.3 μl of water (100 v/v) |
F2 |
| pH 8.5 | 200.0 μl of Lithium sulfate (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F3 |
| pH 4.5 | 200.0 μl of Lithium sulfate (1 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 266.7 μl of water (100 v/v) |
F4 |
| pH 6.5 | 33.3 μl of MES pH 6.5 (3 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v) 666.7 μl of water (100 v/v) |
F5 |
| pH 5.5 | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 5.5 (1 M) 400.0 μl of water (100 v/v) |
F6 |
| pH 8.5 | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 100.0 μl of SPG buffer pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100.0 μl of MMT buffer pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
F8 |
| pH 9 | 100.0 μl of MMT buffer pH 9 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
F9 |
| pH 5 | 100.0 μl of MIB buffer pH 5 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
F10 |
| pH 7 | 100.0 μl of PCB buffer pH 7 (1 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
F11 |
| pH 5.5 | 15.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 240.0 μl of Polyethylene glycol 1,500 (50 w/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 500.0 μl of Sodium chloride (5 M) 211.7 μl of water (100 v/v) |
F12 |
| NA | 800.0 μl of di-Sodium malonate (3 M) 200.0 μl of water (100 v/v) |
G1 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v) |
G2 |
| pH 5.5 | 66.7 μl of Ammonium sulfate (3 M) 200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 33.3 μl of Sodium acetate pH 5.5 (3 M) 700.0 μl of water (100 v/v) |
G3 |
| pH 8.5 | 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G4 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 250.0 μl of water (100 v/v) |
G5 |
| pH 4 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 100.0 μl of tri-Sodium citrate (2 M) 400.0 μl of water (100 v/v) |
G6 |
| pH 8.5 | 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate (3 M) 433.3 μl of water (100 v/v) |
G7 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 8.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 492.0 μl of water (100 v/v) |
G8 |
| NA | 700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v) |
G9 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Sodium di-hydrogen phosphate (4 M) 160.0 μl of di-Potassium hydrogen phosphate (5 M) 540.0 μl of water (100 v/v) |
G10 |
| pH 6 | 40.0 μl of Ammonium chloride (5 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 460.0 μl of water (100 v/v) |
G11 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 460.0 μl of water (100 v/v) |
G12 |
| pH 8 | 200.0 μl of Lithium chloride (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 300.0 μl of water (100 v/v) |
H1 |
| pH 8.5 | 100.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
H2 |
| pH 8.5 | 500.0 μl of Ethylene glycol (100 v/v) 80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
H3 |
| pH 6.5 | 30.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 470.0 μl of water (100 v/v) |
H4 |
| pH 8.5 | 40.0 μl of Magnesium chloride (2.5 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 260.0 μl of water (100 v/v) |
H5 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 333.3 μl of water (100 v/v) |
H6 |
| pH 4.5 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 33.3 μl of Sodium acetate pH 4.5 (3 M) 166.7 μl of water (100 v/v) |
H7 |
| pH 6.5 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of Imidazole pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |
H8 |
| pH 8.5 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 160.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 340.0 μl of water (100 v/v) |
H9 |
| pH 6.5 | 400.0 μl of 2-Propanol (100 v/v) 100.0 μl of Imidazole pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of water (100 v/v) |
H10 |
| pH 8.5 | 300.0 μl of 2-Propanol (100 v/v) 600.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) |
H11 |
| pH 5.5 | 20.0 μl of Ammonium acetate (5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 540.0 μl of water (100 v/v) |
H12 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 300.0 μl of Polyethylene glycol 20,000 (50 w/v) 600.0 μl of water (100 v/v) |