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Supplemental Information

Screen Description

Wizard III&IV

Wizard / Wizard III&IV
Created 2013-01-04.

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Barcodes

Wizard III&IV

No barcodes found.

Screen Structure

Wizard III&IV

Well Count 96
Number of distinct ingredients 69
Internal diversity (score mean dist) 0.961

Screen Composition

2025-08-16T14:27:59.565895 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Wizard III&IV
Buffer 99
Cryoprotectant 7
Organic (non-volatile) 32
Organic (volatile) 11
Polymer 74
Salt 91

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Wizard III&IV Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
520.0 μl of water (100 v/v)
A2
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.6
  • Calcium chloride 0.02 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
20.0 μl of Calcium chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
580.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
40.0 μl of Ammonium formate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium chloride 0.2 M -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
A6
  • Polyethylene glycol 3,350 20 w/v -
  • Barium chloride 0.2 M -
NA
200.0 μl of Barium chloride (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
A7
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium di-hydrogen phosphate 0.2 M -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
A8
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium nitrate (1 M)
400.0 μl of water (100 v/v)
A9
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
633.3 μl of water (100 v/v)
A10
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 8,000 10 w/v -
  • HEPES 0.1 M pH 7.5
  • Ethylene glycol 8 v/v -
pH 7.5
80.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
620.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 4,000 8 w/v -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
B3
  • tri-Sodium citrate 1.6 M -
NA
800.0 μl of tri-Sodium citrate (2 M)
200.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Potassium citrate (2.5 M)
520.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 4,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-Propanol 10 v/v -
pH 5.5
100.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 4
  • Lithium chloride 1 M -
pH 4
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
300.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
NA
20.0 μl of Ammonium nitrate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 6,000 10 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
B9
  • Sodium di-hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
  • di-Potassium hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
160.0 μl of di-Potassium hydrogen phosphate (5 M)
540.0 μl of water (100 v/v)
B10
  • Ethanol 20 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Ethanol (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 20,000 10 w/v -
  • BICINE 0.1 M pH 9
  • 1,4-Dioxane 2 v/v -
pH 9
40.0 μl of 1,4-Dioxane (50 v/v)
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 20,000 (50 w/v)
660.0 μl of water (100 v/v)
B12
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C1
  • Polyethylene glycol 1,000 10 w/v -
  • Polyethylene glycol 8,000 10 w/v -
NA
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
600.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 1,500 24 w/v -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7.5
  • Magnesium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
C4
  • 2-methyl-2,4-pentanediol BCC 70 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of water (100 v/v)
C5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium sulfate 0.17 M -
  • Glycerol 15 v/v -
NA
56.7 μl of Ammonium sulfate (3 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
283.3 μl of water (100 v/v)
C7
  • 2-Propanol 14 v/v -
  • Sodium acetate 0.07 M pH 4.6
  • Calcium chloride 0.14 M -
  • Glycerol 30 v/v -
pH 4.6
140.0 μl of 2-Propanol (100 v/v)
140.0 μl of Calcium chloride (1 M)
300.0 μl of Glycerol (100 v/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
350.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 8,000 16 w/v -
  • Potassium di-hydrogen phosphate 0.04 M -
  • Glycerol 20 v/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Potassium di-hydrogen phosphate (2 M)
460.0 μl of water (100 v/v)
C9
  • Magnesium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
33.3 μl of MES pH 6.5 (3 M)
800.0 μl of Magnesium sulfate (2 M)
166.7 μl of water (100 v/v)
C10
  • Polyethylene glycol 6,000 10 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
700.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 8,000 14.4 w/v -
  • Sodium cacodylate 0.08 M pH 6.5
  • Calcium acetate 0.16 M -
  • Glycerol 20 v/v -
pH 6.5
160.0 μl of Calcium acetate (1 M)
200.0 μl of Glycerol (100 v/v)
288.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
272.0 μl of water (100 v/v)
C12
  • Jeffamine® M-600 30 v/v -
  • MES 0.1 M pH 6.5
  • Cesium chloride 0.05 M -
pH 6.5
50.0 μl of Cesium chloride (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
33.3 μl of MES pH 6.5 (3 M)
316.7 μl of water (100 v/v)
D1
  • Ammonium sulfate 3.2 M -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
800.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 10,000 15 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • 1,4-Dioxane 2 v/v -
pH 5.5
40.0 μl of 1,4-Dioxane (50 v/v)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
560.0 μl of water (100 v/v)
D3
  • Jeffamine® M-600 20 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
500.0 μl of water (100 v/v)
D4
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 9 (1 M)
800.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 28 v/v -
  • HEPES 0.1 M pH 7.5
  • Calcium chloride 0.2 M -
pH 7.5
200.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
420.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
80.0 μl of Lithium sulfate (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
D8
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Lithium sulfate 0.2 M -
pH 8
80.0 μl of Lithium sulfate (2.5 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
220.0 μl of water (100 v/v)
D9
  • Ammonium sulfate 1.5 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol 12 v/v -
pH 8.5
500.0 μl of Ammonium sulfate (3 M)
120.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
280.0 μl of water (100 v/v)
D10
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium chloride 0.2 M -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
40.0 μl of Ammonium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
D11
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
33.3 μl of MES pH 6.5 (3 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
300.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 10,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
E1
  • Glycerol 20 v/v -
  • di-Potassium hydrogen phosphate 0.04 M -
  • Polyethylene glycol 8,000 16 w/v -
NA
200.0 μl of Glycerol (100 v/v)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
8.0 μl of di-Potassium hydrogen phosphate (5 M)
472.0 μl of water (100 v/v)
E2
  • Ethanol 15 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 8
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
300.0 μl of Ethanol (50 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
E3
  • Ethanol 40 v/v -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 5 w/v -
pH 4.2
800.0 μl of Ethanol (50 v/v)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
E4
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
833.3 μl of water (100 v/v)
E5
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 400 2 v/v -
pH 5.5
666.7 μl of Ammonium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate pH 5.5 (3 M)
280.0 μl of water (100 v/v)
E6
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
633.3 μl of water (100 v/v)
E7
  • Lithium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Magnesium sulfate 0.1 M -
  • 2-Propanol 5 v/v -
pH 4.5
50.0 μl of 2-Propanol (100 v/v)
666.7 μl of Lithium sulfate (3 M)
100.0 μl of Magnesium sulfate (1 M)
33.3 μl of Sodium acetate pH 4.5 (3 M)
150.0 μl of water (100 v/v)
E8
  • Lithium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 2 v/v -
pH 8.5
666.7 μl of Lithium sulfate (3 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
213.3 μl of water (100 v/v)
E9
  • Lithium sulfate 2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Magnesium sulfate 0.1 M -
  • Polyethylene glycol 400 5 v/v -
pH 5.5
666.7 μl of Lithium sulfate (3 M)
100.0 μl of Magnesium sulfate (1 M)
50.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate pH 5.5 (3 M)
150.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 200 50 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Magnesium chloride 0.2 M -
pH 6.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
320.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 300 40 v/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Calcium acetate 0.2 M -
pH 6.5
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
E12
  • Jeffamine® M-600 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
F1
  • di-Sodium succinate 0.8 M -
NA
266.7 μl of di-Sodium succinate pH 7 (3 M)
733.3 μl of water (100 v/v)
F2
  • Polyethylene glycol 400 40 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
200.0 μl of Lithium sulfate (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 400 50 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
pH 4.5
200.0 μl of Lithium sulfate (1 M)
500.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate pH 4.5 (3 M)
266.7 μl of water (100 v/v)
F4
  • Polyethylene glycol monomethyl ether 550 15 v/v -
  • MES 0.1 M pH 6.5
pH 6.5
33.3 μl of MES pH 6.5 (3 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
666.7 μl of water (100 v/v)
F5
  • Polyethylene glycol 1,500 25 w/v -
  • SPG buffer 0.1 M pH 5.5
pH 5.5
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of SPG buffer pH 5.5 (1 M)
400.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 1,500 25 w/v -
  • SPG buffer 0.1 M pH 8.5
pH 8.5
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of SPG buffer pH 8.5 (1 M)
400.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 6.5
pH 6.5
100.0 μl of MMT buffer pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 1,500 25 w/v -
  • MMT buffer 0.1 M pH 9
pH 9
100.0 μl of MMT buffer pH 9 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 1,500 25 w/v -
  • MIB buffer 0.1 M pH 5
pH 5
100.0 μl of MIB buffer pH 5 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 1,500 25 w/v -
  • PCB buffer 0.1 M pH 7
pH 7
100.0 μl of PCB buffer pH 7 (1 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 1,500 12 w/v -
  • Sodium acetate 0.1 M pH 5.5
  • Sodium chloride 2.5 M -
  • 2-methyl-2,4-pentanediol BCC 1.5 v/v -
pH 5.5
15.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
240.0 μl of Polyethylene glycol 1,500 (50 w/v)
33.3 μl of Sodium acetate pH 5.5 (3 M)
500.0 μl of Sodium chloride (5 M)
211.7 μl of water (100 v/v)
F12
  • di-Sodium malonate 2.4 M -
NA
800.0 μl of di-Sodium malonate (3 M)
200.0 μl of water (100 v/v)
G1
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
G2
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
  • Sodium acetate 0.1 M pH 5.5
  • Ammonium sulfate 0.2 M -
pH 5.5
66.7 μl of Ammonium sulfate (3 M)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
33.3 μl of Sodium acetate pH 5.5 (3 M)
700.0 μl of water (100 v/v)
G3
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Trimethylamine N-oxide 0.2 M -
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 3,350 20 w/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Sodium fluoride 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
250.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Sodium citrate 0.1 M pH 4
  • tri-Sodium citrate 0.2 M -
pH 4
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
100.0 μl of tri-Sodium citrate (2 M)
400.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 3,350 20 w/v -
  • BIS-TRIS propane 0.1 M pH 8.5
  • di-Sodium malonate 0.2 M -
pH 8.5
100.0 μl of BIS-TRIS propane pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of di-Sodium malonate (3 M)
433.3 μl of water (100 v/v)
G7
  • Poly(acrylic acid sodium salt) 5,100 20 w/v -
  • HEPES 0.1 M pH 7
  • Magnesium chloride 0.02 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
8.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
492.0 μl of water (100 v/v)
G8
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate pH 7 (3 M)
300.0 μl of water (100 v/v)
G9
  • di-Potassium hydrogen phosphate 0.8 M -
  • HEPES 0.1 M pH 7.5
  • Sodium di-hydrogen phosphate 0.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
160.0 μl of di-Potassium hydrogen phosphate (5 M)
540.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 6
  • Ammonium chloride 0.2 M -
pH 6
40.0 μl of Ammonium chloride (5 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
460.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 6,000 20 w/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 6,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Lithium chloride 0.2 M -
pH 8
200.0 μl of Lithium chloride (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
H1
  • Polyvinylpyrrolidone K15 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Cobalt (II) chloride 0.1 M -
pH 8.5
100.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H2
  • Ethylene glycol 50 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
500.0 μl of Ethylene glycol (100 v/v)
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 8,000 20 w/v -
  • Imidazole 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 3 v/v -
pH 6.5
30.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
470.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.1 M -
  • Polyethylene glycol 400 20 v/v -
pH 8.5
40.0 μl of Magnesium chloride (2.5 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
260.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 8,000 20 w/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.2 M -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
H6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 1,500 25 w/v -
pH 4.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
33.3 μl of Sodium acetate pH 4.5 (3 M)
166.7 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Imidazole 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 10 w/v -
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of Imidazole pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 0.5 M -
  • Polyethylene glycol 8,000 8 w/v -
pH 8.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
160.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
340.0 μl of water (100 v/v)
H9
  • 2-Propanol 40 v/v -
  • Imidazole 0.1 M pH 6.5
  • Polyethylene glycol 8,000 15 w/v -
pH 6.5
400.0 μl of 2-Propanol (100 v/v)
100.0 μl of Imidazole pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of water (100 v/v)
H10
  • 2-Propanol 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 30 w/v -
pH 8.5
300.0 μl of 2-Propanol (100 v/v)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
H11
  • Polyethylene glycol 10,000 17 w/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium acetate 0.1 M -
pH 5.5
20.0 μl of Ammonium acetate (5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
540.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 20,000 15 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 20,000 (50 w/v)
600.0 μl of water (100 v/v)