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Supplemental Information

Screen Description

Wizard III&IV

Wizard / Wizard III&IV
Created 2013-01-04.

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Wizard III&IV

    Screen Structure

    Wizard III&IV

    Well Count 96
    Number of distinct ingredients 69
    Internal diversity (score mean dist) 0.961

    Screen Composition

    2025-06-14T14:05:16.650349 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Wizard III&IV
    Buffer 99
    Cryoprotectant 7
    Organic (non-volatile) 32
    Organic (volatile) 11
    Polymer 74
    Salt 91

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Wizard III&IV Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 3,350 20 w/v -
    • di-Ammonium hydrogen citrate 0.2 M -
    NA
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    A2
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.6
    • Calcium chloride 0.02 M -
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    20 μl of Calcium chloride (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    580 μl of water (100 v/v)
    A3
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium formate 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    A4
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium formate 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Ammonium formate (5 M)
    560 μl of water (100 v/v)
    A5
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium chloride 0.2 M -
    NA
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    560 μl of water (100 v/v)
    A6
    • Polyethylene glycol 3,350 20 w/v -
    • Barium chloride 0.2 M -
    NA
    200 μl of Barium chloride (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A7
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium di-hydrogen phosphate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of Ammonium di-hydrogen phosphate (3 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    333 μl of water (100 v/v)
    A8
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium nitrate 0.2 M -
    NA
    200 μl of Potassium nitrate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    A9
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    pH 4
    266 μl of Ammonium sulfate (3 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    633 μl of water (100 v/v)
    A10
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium thiocyanate 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate (8 M)
    575 μl of water (100 v/v)
    A11
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 9
    pH 9
    100 μl of BICINE pH 9 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    500 μl of water (100 v/v)
    A12
    • Polyethylene glycol 8,000 10 w/v -
    • HEPES 0.1 M pH 7.5
    • Ethylene glycol 8 v/v -
    pH 7.5
    80 μl of Ethylene glycol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    620 μl of water (100 v/v)
    B1
    • Polyethylene glycol 4,000 8 w/v -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    740 μl of water (100 v/v)
    B2
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5
    pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of water (100 v/v)
    B3
    • tri-Sodium citrate 1.6 M -
    NA
    800 μl of tri-Sodium citrate (2 M)
    200 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Potassium citrate 0.2 M -
    NA
    80 μl of tri-Potassium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    B5
    • Polyethylene glycol 4,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • 2-Propanol 10 v/v -
    pH 5.5
    100 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of water (100 v/v)
    B6
    • Polyethylene glycol 6,000 20 w/v -
    • tri-Sodium citrate 0.1 M pH 4
    • Lithium chloride 1 M -
    pH 4
    200 μl of Lithium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    300 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,350 20 w/v -
    • Ammonium nitrate 0.2 M -
    NA
    20 μl of Ammonium nitrate (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    B8
    • Polyethylene glycol 6,000 10 w/v -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    B9
    • Sodium di-hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    • di-Potassium hydrogen phosphate 0.8 M -
    pH 7.5
    160 μl of di-Potassium hydrogen phosphate (5 M)
    200 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    540 μl of water (100 v/v)
    B10
    • Ethanol 20 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Ethanol (50 v/v)
    500 μl of water (100 v/v)
    B11
    • Polyethylene glycol 20,000 10 w/v -
    • BICINE 0.1 M pH 9
    • 1,4-Dioxane 2 v/v -
    pH 9
    40 μl of 1,4-Dioxane (50 v/v)
    200 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    660 μl of water (100 v/v)
    B12
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    666 μl of Ammonium sulfate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    233 μl of water (100 v/v)
    C1
    • Polyethylene glycol 1,000 10 w/v -
    • Polyethylene glycol 8,000 10 w/v -
    NA
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    600 μl of water (100 v/v)
    C2
    • Polyethylene glycol 1,500 24 w/v -
    • Glycerol 20 v/v -
    NA
    480 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Glycerol (100 v/v)
    320 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Magnesium chloride 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    C4
    • 2-methyl-2,4-pentanediol BCC 70 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    200 μl of water (100 v/v)
    C5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    C6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Ammonium sulfate 0.17 M -
    • Glycerol 15 v/v -
    NA
    150 μl of Glycerol (100 v/v)
    56 μl of Ammonium sulfate (3 M)
    510 μl of Polyethylene glycol 4,000 (50 w/v)
    283 μl of water (100 v/v)
    C7
    • 2-Propanol 14 v/v -
    • Sodium acetate 0.07 M pH 4.6
    • Calcium chloride 0.14 M -
    • Glycerol 30 v/v -
    pH 4.6
    300 μl of Glycerol (100 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    140 μl of 2-Propanol (100 v/v)
    140 μl of Calcium chloride (1 M)
    350 μl of water (100 v/v)
    C8
    • Polyethylene glycol 8,000 16 w/v -
    • Potassium di-hydrogen phosphate 0.04 M -
    • Glycerol 20 v/v -
    NA
    200 μl of Glycerol (100 v/v)
    20 μl of Potassium di-hydrogen phosphate (2 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    460 μl of water (100 v/v)
    C9
    • Magnesium sulfate 1.6 M -
    • MES 0.1 M pH 6.5
    pH 6.5
    33 μl of MES pH 6.5 (3 M)
    800 μl of Magnesium sulfate (2 M)
    166 μl of water (100 v/v)
    C10
    • Polyethylene glycol 6,000 10 w/v -
    • BICINE 0.1 M pH 9
    pH 9
    100 μl of BICINE pH 9 (1 M)
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    700 μl of water (100 v/v)
    C11
    • Polyethylene glycol 8,000 14.4 w/v -
    • Sodium cacodylate 0.08 M pH 6.5
    • Calcium acetate 0.16 M -
    • Glycerol 20 v/v -
    pH 6.5
    200 μl of Glycerol (100 v/v)
    288 μl of Polyethylene glycol 8,000 (50 w/v)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    160 μl of Calcium acetate (1 M)
    272 μl of water (100 v/v)
    C12
    • Jeffamine® M-600 30 v/v -
    • MES 0.1 M pH 6.5
    • Cesium chloride 0.05 M -
    pH 6.5
    50 μl of Cesium chloride (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    33 μl of MES pH 6.5 (3 M)
    316 μl of water (100 v/v)
    D1
    • Ammonium sulfate 3.2 M -
    • tri-Sodium citrate 0.1 M pH 5
    pH 5
    800 μl of Ammonium sulfate (4 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of water (100 v/v)
    D2
    • Polyethylene glycol 10,000 15 w/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • 1,4-Dioxane 2 v/v -
    pH 5.5
    40 μl of 1,4-Dioxane (50 v/v)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    560 μl of water (100 v/v)
    D3
    • Jeffamine® M-600 20 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Jeffamine® M-600 pH 7 (50 v/v)
    500 μl of water (100 v/v)
    D4
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • BICINE 0.1 M pH 9
    pH 9
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BICINE pH 9 (1 M)
    800 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 28 v/v -
    • HEPES 0.1 M pH 7.5
    • Calcium chloride 0.2 M -
    pH 7.5
    200 μl of Calcium chloride (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    420 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    220 μl of water (100 v/v)
    D7
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of water (100 v/v)
    D8
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium sulfate 0.2 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    220 μl of water (100 v/v)
    D9
    • Ammonium sulfate 1.5 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol 12 v/v -
    pH 8.5
    120 μl of Glycerol (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ammonium sulfate (3 M)
    280 μl of water (100 v/v)
    D10
    • 2-methyl-2,4-pentanediol BCC 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium chloride 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Ammonium chloride (5 M)
    500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    360 μl of water (100 v/v)
    D11
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    pH 6.5
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    33 μl of MES pH 6.5 (3 M)
    300 μl of water (100 v/v)
    D12
    • Polyethylene glycol 10,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    E1
    • Glycerol 20 v/v -
    • di-Potassium hydrogen phosphate 0.04 M -
    • Polyethylene glycol 8,000 16 w/v -
    NA
    200 μl of Glycerol (100 v/v)
    8 μl of di-Potassium hydrogen phosphate (5 M)
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    472 μl of water (100 v/v)
    E2
    • Ethanol 15 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    pH 8
    300 μl of Ethanol (50 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    20 μl of Sodium chloride (5 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    530 μl of water (100 v/v)
    E3
    • Ethanol 40 v/v -
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 1,000 5 w/v -
    pH 4.2
    800 μl of Ethanol (50 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    E4
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    pH 5.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    833 μl of water (100 v/v)
    E5
    • Ammonium sulfate 2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol 400 2 v/v -
    pH 5.5
    33 μl of Sodium acetate pH 5.5 (3 M)
    666 μl of Ammonium sulfate (3 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    280 μl of water (100 v/v)
    E6
    • Ammonium sulfate 0.8 M -
    • tri-Sodium citrate 0.1 M pH 4
    pH 4
    266 μl of Ammonium sulfate (3 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    633 μl of water (100 v/v)
    E7
    • Lithium sulfate 2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Magnesium sulfate 0.1 M -
    • 2-Propanol 5 v/v -
    pH 4.5
    33 μl of Sodium acetate pH 4.5 (3 M)
    50 μl of 2-Propanol (100 v/v)
    666 μl of Lithium sulfate (3 M)
    100 μl of Magnesium sulfate (1 M)
    150 μl of water (100 v/v)
    E8
    • Lithium sulfate 2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 2 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Lithium sulfate (3 M)
    20 μl of Polyethylene glycol 400 (100 v/v)
    213 μl of water (100 v/v)
    E9
    • Lithium sulfate 2 M -
    • Sodium acetate 0.1 M pH 5.5
    • Magnesium sulfate 0.1 M -
    • Polyethylene glycol 400 5 v/v -
    pH 5.5
    33 μl of Sodium acetate pH 5.5 (3 M)
    666 μl of Lithium sulfate (3 M)
    100 μl of Magnesium sulfate (1 M)
    50 μl of Polyethylene glycol 400 (100 v/v)
    150 μl of water (100 v/v)
    E10
    • Polyethylene glycol 200 50 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Magnesium chloride 0.2 M -
    pH 6.5
    500 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    E11
    • Polyethylene glycol 300 40 v/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Calcium acetate 0.2 M -
    pH 6.5
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Calcium acetate (1 M)
    300 μl of water (100 v/v)
    E12
    • Jeffamine® M-600 30 v/v -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    300 μl of water (100 v/v)
    F1
    • di-Sodium succinate 0.8 M -
    NA
    266 μl of di-Sodium succinate pH 7 (3 M)
    733 μl of water (100 v/v)
    F2
    • Polyethylene glycol 400 40 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Lithium sulfate (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    300 μl of water (100 v/v)
    F3
    • Polyethylene glycol 400 50 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    pH 4.5
    33 μl of Sodium acetate pH 4.5 (3 M)
    200 μl of Lithium sulfate (1 M)
    500 μl of Polyethylene glycol 400 (100 v/v)
    266 μl of water (100 v/v)
    F4
    • Polyethylene glycol monomethyl ether 550 15 v/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    300 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    33 μl of MES pH 6.5 (3 M)
    666 μl of water (100 v/v)
    F5
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 5.5
    pH 5.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of SPG buffer pH 5.5 (1 M)
    400 μl of water (100 v/v)
    F6
    • Polyethylene glycol 1,500 25 w/v -
    • SPG buffer 0.1 M pH 8.5
    pH 8.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of SPG buffer pH 8.5 (1 M)
    400 μl of water (100 v/v)
    F7
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 6.5
    pH 6.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 6.5 (1 M)
    400 μl of water (100 v/v)
    F8
    • Polyethylene glycol 1,500 25 w/v -
    • MMT buffer 0.1 M pH 9
    pH 9
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MMT buffer pH 9 (1 M)
    400 μl of water (100 v/v)
    F9
    • Polyethylene glycol 1,500 25 w/v -
    • MIB buffer 0.1 M pH 5
    pH 5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MIB buffer pH 5 (1 M)
    400 μl of water (100 v/v)
    F10
    • Polyethylene glycol 1,500 25 w/v -
    • PCB buffer 0.1 M pH 7
    pH 7
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of PCB buffer pH 7 (1 M)
    400 μl of water (100 v/v)
    F11
    • Polyethylene glycol 1,500 12 w/v -
    • Sodium acetate 0.1 M pH 5.5
    • Sodium chloride 2.5 M -
    • 2-methyl-2,4-pentanediol BCC 1.5 v/v -
    pH 5.5
    240 μl of Polyethylene glycol 1,500 (50 w/v)
    33 μl of Sodium acetate pH 5.5 (3 M)
    500 μl of Sodium chloride (5 M)
    15 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    211 μl of water (100 v/v)
    F12
    • di-Sodium malonate 2.4 M -
    NA
    800 μl of di-Sodium malonate (3 M)
    200 μl of water (100 v/v)
    G1
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    • Potassium bromide 0.15 M -
    NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    37 μl of Potassium bromide (4 M)
    362 μl of water (100 v/v)
    G2
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • Sodium acetate 0.1 M pH 5.5
    • Ammonium sulfate 0.2 M -
    pH 5.5
    33 μl of Sodium acetate pH 5.5 (3 M)
    66 μl of Ammonium sulfate (3 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    700 μl of water (100 v/v)
    G3
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Trimethylamine N-oxide 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Trimethylamine N-oxide (1 M)
    300 μl of water (100 v/v)
    G4
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • Sodium fluoride 0.2 M -
    pH 6.5
    250 μl of Sodium fluoride (0.8 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of water (100 v/v)
    G5
    • Polyethylene glycol 3,350 20 w/v -
    • tri-Sodium citrate 0.1 M pH 4
    • tri-Sodium citrate 0.2 M -
    pH 4
    100 μl of tri-Sodium citrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    400 μl of water (100 v/v)
    G6
    • Polyethylene glycol 3,350 20 w/v -
    • BIS-TRIS propane 0.1 M pH 8.5
    • di-Sodium malonate 0.2 M -
    pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS propane pH 8.5 (1 M)
    66 μl of di-Sodium malonate (3 M)
    433 μl of water (100 v/v)
    G7
    • Poly(acrylic acid sodium salt) 5,100 20 w/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.02 M -
    pH 7
    400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    8 μl of Magnesium chloride (2.5 M)
    492 μl of water (100 v/v)
    G8
    • di-Sodium DL-malate 2.1 M -
    NA
    700 μl of di-Sodium DL-malate pH 7 (3 M)
    300 μl of water (100 v/v)
    G9
    • di-Potassium hydrogen phosphate 0.8 M -
    • HEPES 0.1 M pH 7.5
    • Sodium di-hydrogen phosphate 0.8 M -
    pH 7.5
    160 μl of di-Potassium hydrogen phosphate (5 M)
    200 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    540 μl of water (100 v/v)
    G10
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    • Ammonium chloride 0.2 M -
    pH 6
    40 μl of Ammonium chloride (5 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    460 μl of water (100 v/v)
    G11
    • Polyethylene glycol 6,000 20 w/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.2 M -
    pH 7
    40 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    460 μl of water (100 v/v)
    G12
    • Polyethylene glycol 6,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium chloride 0.2 M -
    pH 8
    200 μl of Lithium chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    300 μl of water (100 v/v)
    H1
    • Polyvinylpyrrolidone K15 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Cobalt (II) chloride 0.1 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Cobalt (II) chloride (1 M)
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    400 μl of water (100 v/v)
    H2
    • Ethylene glycol 50 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of Ethylene glycol (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    320 μl of water (100 v/v)
    H3
    • Polyethylene glycol 8,000 20 w/v -
    • Imidazole 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 3 v/v -
    pH 6.5
    100 μl of Imidazole pH 6.5 (1 M)
    30 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    470 μl of water (100 v/v)
    H4
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 400 20 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    40 μl of Magnesium chloride (2.5 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    260 μl of water (100 v/v)
    H5
    • Polyethylene glycol 8,000 20 w/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    • 2-Propanol 10 v/v -
    pH 7.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of water (100 v/v)
    H6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 1,500 25 w/v -
    pH 4.5
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    33 μl of Sodium acetate pH 4.5 (3 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    166 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Imidazole 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 3,350 10 w/v -
    pH 6.5
    66 μl of Ammonium sulfate (3 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Imidazole pH 6.5 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    333 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 0.5 M -
    • Polyethylene glycol 8,000 8 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Sodium chloride (5 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    160 μl of Polyethylene glycol 8,000 (50 w/v)
    340 μl of water (100 v/v)
    H9
    • 2-Propanol 40 v/v -
    • Imidazole 0.1 M pH 6.5
    • Polyethylene glycol 8,000 15 w/v -
    pH 6.5
    400 μl of 2-Propanol (100 v/v)
    100 μl of Imidazole pH 6.5 (1 M)
    300 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of water (100 v/v)
    H10
    • 2-Propanol 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 30 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of 2-Propanol (100 v/v)
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    H11
    • Polyethylene glycol 10,000 17 w/v -
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium acetate 0.1 M -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    20 μl of Ammonium acetate (5 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    540 μl of water (100 v/v)
    H12
    • Polyethylene glycol 20,000 15 w/v -
    • HEPES 0.1 M pH 7
    pH 7
    300 μl of Polyethylene glycol 20,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    600 μl of water (100 v/v)