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Supplemental Information

Screen Description

Microlytic-Anatrace / SuperCOMBI
Created 2013-03-25.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 81
Internal diversity (Score mean dist)0.92
Internal diversity (Ion mean dist)0.89
Internal diversity (PEG mean dist)0.89
Internal diversity (Known pH mean dist)0.81
Internal diversity (Penalty mean dist)0.91

Screen Composition

Chemical classes bar chart
ingredient type SuperCOMBI
Buffer 107
Cryoprotectant 3
Organic (non-volatile) 11
Polymer 91
Salt 113

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
SuperCOMBI Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • Ammonium sulfate 1.5 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 6.5
pH 6.5
416.67 μl of Ammonium sulfate (3.6 M)
20.0 μl of Sodium chloride (5 M)
28.21 μl of tris chloride BCC pH 6.3 (3 M)
5.12 μl of tris chloride BCC pH 7 (3 M)
530.0 μl of water (100 v/v)
A2
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
A3
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 1,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
pH 6.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
33.33 μl of Sodium Potassium phosphate pH 6.2 (3 M)
526.67 μl of water (100 v/v)
A5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
A6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium thiocyanate 0.1 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
A7
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Potassium bromide 0.15 M -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
A8
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
A9
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 300 40 v/v -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
A12
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
55.56 μl of Ammonium sulfate (3.6 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
344.44 μl of water (100 v/v)
B1
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.33 μl of Potassium Sodium tartrate (1.5 M)
466.67 μl of water (100 v/v)
B9
  • Polyethylene glycol 3,350 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium iodide 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium iodide (1 M)
400.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium acetate 0.2 M -
NA
40.0 μl of Lithium acetate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium chloride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 400 30 v/v -
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.5
pH 6.5
40.0 μl of Magnesium chloride (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 400 30 v/v -
  • CAPS 0.1 M pH 10.5
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium acetate 0.17 M -
  • Glycerol 15 v/v -
  • tri-Sodium citrate 0.085 M pH 5.6
pH 5.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
85.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Sodium acetate 0.17 M -
  • Glycerol 15 v/v -
  • tris chloride BCC 0.085 M pH 8.5
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate (2 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
170.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 4,000 24 w/v -
  • Magnesium chloride 0.16 M -
  • Glycerol 20 v/v -
  • tris chloride BCC 0.08 M pH 8.5
pH 8.5
200.0 μl of Glycerol (100 v/v)
64.0 μl of Magnesium chloride (2.5 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
176.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 4,000 25 w/v -
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Calcium chloride (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
55.56 μl of Ammonium sulfate (3.6 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
344.44 μl of water (100 v/v)
C9
  • Polyethylene glycol 4,000 32 w/v -
  • Lithium chloride 0.8 M -
NA
80.0 μl of Lithium chloride (10 M)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
280.0 μl of water (100 v/v)
C10
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
C11
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
pH 6.5
55.56 μl of Ammonium sulfate (3.6 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
244.44 μl of water (100 v/v)
C12
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Calcium chloride 0.05 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
D1
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Magnesium chloride 0.05 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
580.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 600 40 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
800.0 μl of Polyethylene glycol 600 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
100.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 6,000 30 w/v -
  • Lithium chloride 1 M -
  • Sodium acetate 0.1 M -
NA
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 8,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 8,000 20 w/v -
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
pH 6
200.0 μl of Calcium acetate (1 M)
33.33 μl of MES pH 6 (3 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
366.67 μl of water (100 v/v)
D6
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
55.56 μl of Ammonium sulfate (3.6 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
244.44 μl of water (100 v/v)
D9
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
  • Ammonium sulfate 0.05 M -
  • BIS-TRIS 0.05 M pH 6.5
pH 6.5
13.89 μl of Ammonium sulfate (3.6 M)
50.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
336.11 μl of water (100 v/v)
D10
  • Polypropylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polypropylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D11
  • Polypropylene glycol 400 30 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polypropylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D12
  • Sodium chloride 2.5 M -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
320.0 μl of water (100 v/v)
E1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium chloride 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
60.0 μl of Ammonium chloride (5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
390.0 μl of water (100 v/v)
E2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Potassium nitrate 0.1 M -
  • Ammonium formate 0.3 M pH 6.6
pH 6.6
30.0 μl of Ammonium formate pH 6.6 (10 M)
33.33 μl of Potassium nitrate (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
486.67 μl of water (100 v/v)
E3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium nitrate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 5.9
pH 5.9
30.0 μl of Ammonium nitrate (10 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.9 (1 M)
420.0 μl of water (100 v/v)
E4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium di-hydrogen phosphate 0.3 M -
  • MES 0.1 M pH 5.7
pH 5.7
120.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of MES pH 5.7 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
330.0 μl of water (100 v/v)
E5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium sulfate 0.3 M -
  • BIS-TRIS 0.1 M pH 6.8
pH 6.8
85.71 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
364.29 μl of water (100 v/v)
E6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium tartrate 0.3 M -
  • Sodium acetate 0.1 M pH 5.8
pH 5.8
150.0 μl of di-Ammonium tartrate (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
15.1 μl of Sodium acetate pH 5 (1 M)
84.9 μl of Sodium acetate pH 6.2 (1 M)
300.0 μl of water (100 v/v)
E7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Cadmium chloride 0.3 M -
  • Sodium acetate 0.1 M pH 3.7
pH 3.7
300.0 μl of Cadmium chloride (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 3.7 (1 M)
150.0 μl of water (100 v/v)
E8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Cadmium sulfate 0.3 M -
  • Imidazole 0.1 M pH 4.6
pH 4.6
300.0 μl of Cadmium sulfate (1 M)
97.04 μl of Imidazole pH 4.5 (1 M)
2.96 μl of Imidazole pH 5.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Cesium chloride 0.3 M -
  • HEPES 0.1 M pH 7.6
pH 7.6
300.0 μl of Cesium chloride (1 M)
100.0 μl of HEPES pH 7.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Calcium acetate 0.3 M -
  • BICINE 0.1 M pH 6.4
pH 6.4
100.0 μl of BICINE pH 6.4 (1 M)
300.0 μl of Calcium acetate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Calcium chloride 0.3 M -
  • BIS-TRIS propane 0.1 M pH 8.5
pH 8.5
100.0 μl of BIS-TRIS propane pH 8.5 (1 M)
150.0 μl of Calcium chloride (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of water (100 v/v)
E12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Cobalt (II) chloride 0.3 M -
  • di-Ammonium tartrate 0.1 M pH 3.2
pH 3.2
300.0 μl of Cobalt (II) chloride (1 M)
33.33 μl of di-Ammonium tartrate pH 3.2 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
216.67 μl of water (100 v/v)
F1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium hydrogen citrate 0.3 M -
  • Magnesium formate 0.1 M -
NA
120.0 μl of di-Ammonium hydrogen citrate pH 4.9 (2.5 M)
100.0 μl of Magnesium formate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
330.0 μl of water (100 v/v)
F2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium cacodylate 0.1 M -
  • di-Sodium DL-malate 0.3 M pH 2.9
pH 2.9
100.0 μl of di-Sodium DL-malate pH 2.9 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium cacodylate (1 M)
350.0 μl of water (100 v/v)
F3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Iron (III) chloride 0.3 M -
  • Potassium nitrate 0.1 M -
NA
300.0 μl of Iron (III) chloride (1 M)
33.33 μl of Potassium nitrate pH 1.1 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
216.67 μl of water (100 v/v)
F4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Lithium chloride 0.3 M -
  • Potassium formate 0.1 M pH 6.3
pH 6.3
30.0 μl of Lithium chloride (10 M)
100.0 μl of Potassium formate pH 6.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
420.0 μl of water (100 v/v)
F5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Lithium sulfate 0.3 M -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
150.0 μl of Lithium sulfate (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of water (100 v/v)
F6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium acetate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4.7
pH 4.7
300.0 μl of Magnesium acetate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.7 (1 M)
150.0 μl of water (100 v/v)
F7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium chloride 0.3 M -
  • Sodium cacodylate 0.1 M pH 5.8
pH 5.8
150.0 μl of Magnesium chloride (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium cacodylate pH 5.8 (1 M)
300.0 μl of water (100 v/v)
F8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium nitrate 0.3 M -
  • HEPES 0.1 M pH 7.4
pH 7.4
100.0 μl of HEPES pH 7.4 (1 M)
100.0 μl of Magnesium nitrate (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
350.0 μl of water (100 v/v)
F9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium sulfate 0.1 M -
  • Potassium formate 0.1 M pH 6.1
pH 6.1
50.0 μl of Magnesium sulfate (2 M)
100.0 μl of Potassium formate pH 6.1 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
400.0 μl of water (100 v/v)
F10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Nickel (II) chloride 0.3 M -
  • MES 0.1 M pH 5.7
pH 5.7
100.0 μl of MES pH 5.7 (1 M)
75.0 μl of Nickel (II) chloride (4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of water (100 v/v)
F11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Potassium bromide 0.3 M -
  • BIS-TRIS 0.1 M pH 6.6
pH 6.6
100.0 μl of BIS-TRIS pH 6.6 (1 M)
75.0 μl of Potassium bromide (4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of water (100 v/v)
F12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Potassium di-hydrogen phosphate 0.3 M -
  • tris chloride BCC 0.1 M pH 6.1
pH 6.1
200.0 μl of Potassium di-hydrogen phosphate (1.5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
24.61 μl of tris chloride BCC pH 6 (3 M)
8.72 μl of tris chloride BCC pH 6.3 (3 M)
316.67 μl of water (100 v/v)
G1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Potassium Sodium tartrate 0.3 M -
  • BICINE 0.1 M pH 8.8
pH 8.8
100.0 μl of BICINE pH 8.8 (1 M)
200.0 μl of Potassium Sodium tartrate (1.5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
250.0 μl of water (100 v/v)
G2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Potassium tartrate 0.3 M -
  • Imidazole 0.1 M pH 7.3
pH 7.3
300.0 μl of di-Potassium tartrate (1 M)
100.0 μl of Imidazole pH 7.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
G3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium tartrate 0.1 M pH 5.8
pH 5.8
25.64 μl of di-Ammonium tartrate pH 5.5 (2 M)
24.36 μl of di-Ammonium tartrate pH 8.5 (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
500.0 μl of water (100 v/v)
G4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium bromide 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
60.0 μl of Sodium bromide (5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
390.0 μl of water (100 v/v)
G5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium chloride 0.3 M -
  • tris chloride BCC 0.1 M pH 8.1
pH 8.1
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
60.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.1 (1 M)
390.0 μl of water (100 v/v)
G6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium fluoride 0.3 M -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of Sodium fluoride (0.8 M)
75.0 μl of water (100 v/v)
G7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium formate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4.5
pH 4.5
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
42.86 μl of Sodium formate (7 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
407.14 μl of water (100 v/v)
G8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium iodide 0.3 M -
  • Sodium acetate 0.1 M pH 4.7
pH 4.7
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 4.7 (1 M)
300.0 μl of Sodium iodide (1 M)
150.0 μl of water (100 v/v)
G9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Sodium malonate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 6.8
pH 6.8
88.24 μl of di-Sodium malonate pH 4 (3.4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
1.83 μl of tri-Sodium citrate pH 5.8 (1 M)
98.17 μl of tri-Sodium citrate pH 7 (1 M)
361.76 μl of water (100 v/v)
G10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium nitrate 0.3 M -
  • MES 0.1 M pH 6.1
pH 6.1
100.0 μl of MES pH 6.1 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
42.86 μl of Sodium nitrate (7 M)
407.14 μl of water (100 v/v)
G11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium di-hydrogen phosphate 0.3 M -
  • BIS-TRIS 0.1 M pH 5.8
pH 5.8
100.0 μl of BIS-TRIS pH 5.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
75.0 μl of Sodium di-hydrogen phosphate (4 M)
375.0 μl of water (100 v/v)
G12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium sulfate 0.3 M -
  • BIS-TRIS propane 0.1 M pH 9.3
pH 9.3
100.0 μl of BIS-TRIS propane pH 9.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of Sodium sulfate (1 M)
150.0 μl of water (100 v/v)
H1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium thiocyanate 0.3 M -
  • HEPES 0.1 M pH 7.8
pH 7.8
100.0 μl of HEPES pH 7.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
37.5 μl of Sodium thiocyanate (8 M)
412.5 μl of water (100 v/v)
H2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Sodium succinate 0.3 M -
  • Imidazole 0.1 M -
NA
300.0 μl of di-Sodium succinate pH 3.4 (1 M)
100.0 μl of Imidazole (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
H3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Trimethylamine N-oxide 0.3 M -
  • BICINE 0.1 M pH 8.6
pH 8.6
100.0 μl of BICINE pH 8.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of Trimethylamine N-oxide (1 M)
150.0 μl of water (100 v/v)
H4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tri-Potassium citrate 0.3 M -
  • BIS-TRIS propane 0.1 M pH 9.8
pH 9.8
100.0 μl of BIS-TRIS propane pH 9.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
120.0 μl of tri-Potassium citrate (2.5 M)
330.0 μl of water (100 v/v)
H5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Jeffamine® M-600 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
250.0 μl of water (100 v/v)
H6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Zinc chloride 0.3 M -
  • Magnesium formate 0.1 M pH 5.7
pH 5.7
100.0 μl of Magnesium formate pH 5.7 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of Zinc chloride (2 M)
300.0 μl of water (100 v/v)
H7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Zinc sulfate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
150.0 μl of Zinc sulfate (2 M)
300.0 μl of water (100 v/v)
H8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • CHES 0.1 M pH 9.9
pH 9.9
47.38 μl of CHES pH 9.5 (1 M)
52.62 μl of CHES pH 10.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Imidazole 0.1 M pH 6.6
pH 6.6
100.0 μl of Imidazole pH 6.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium formate 0.1 M pH 5.9
pH 5.9
100.0 μl of Magnesium formate pH 5.9 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium acetate 0.1 M pH 4.7
pH 4.7
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 4.7 (1 M)
450.0 μl of water (100 v/v)
H12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tris chloride BCC 0.1 M pH 8.1
pH 8.1
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tris chloride BCC pH 8.1 (1 M)
450.0 μl of water (100 v/v)