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Supplemental Information

Screen Description

Microlytic-Anatrace / SuperCOMBI
Created 2013-03-25.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 81
Internal diversity (score mean dist) 0.921

Screen Composition

2025-10-22T00:23:00.475882 image/svg+xml Matplotlib v3.10.6, https://matplotlib.org/
ingredient type SuperCOMBI
Buffer 107
Cryoprotectant 3
Organic (non-volatile) 11
Polymer 91
Salt 113

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
SuperCOMBI Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 6.5
  • Ammonium sulfate 1.5 M -
pH 6.5
416.7 μl of Ammonium sulfate (3.6 M)
20.0 μl of Sodium chloride (5 M)
28.2 μl of tris chloride BCC pH 6.3 (3 M)
5.1 μl of tris chloride BCC pH 7 (3 M)
530.0 μl of water (100 v/v)
A2
  • Ammonium di-hydrogen phosphate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 50 v/v -
pH 8.5
500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
A3
  • Calcium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
A4
  • Sodium chloride 0.2 M -
  • Sodium Potassium phosphate 0.1 M pH 6.2
  • Polyethylene glycol 1,000 20 w/v -
pH 6.2
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
526.7 μl of water (100 v/v)
A5
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
A6
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
A7
  • Potassium bromide 0.15 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)
A8
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
A10
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
500.0 μl of water (100 v/v)
A11
  • Calcium acetate 0.1 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
pH 6.5
100.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
A12
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
55.6 μl of Ammonium sulfate (3.6 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
344.4 μl of water (100 v/v)
B1
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
B2
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
B3
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
B4
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
80.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
B6
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
B7
  • Potassium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B8
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
B9
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
B10
  • Potassium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium iodide (1 M)
400.0 μl of water (100 v/v)
B11
  • Lithium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
40.0 μl of Lithium acetate (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B12
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
C1
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C2
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
40.0 μl of Magnesium chloride (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
C3
  • CAPS 0.1 M pH 10.5
  • Polyethylene glycol 400 30 v/v -
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
C4
  • Ammonium acetate 0.17 M -
  • tri-Sodium citrate 0.085 M pH 5.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 5.6
170.0 μl of Ammonium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
85.0 μl of water (100 v/v)
C5
  • Sodium acetate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
pH 8.5
150.0 μl of Glycerol (100 v/v)
510.0 μl of Polyethylene glycol 4,000 (50 w/v)
85.0 μl of Sodium acetate (2 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
170.0 μl of water (100 v/v)
C6
  • Magnesium chloride 0.16 M -
  • tris chloride BCC 0.08 M pH 8.5
  • Polyethylene glycol 4,000 24 w/v -
  • Glycerol 20 v/v -
pH 8.5
200.0 μl of Glycerol (100 v/v)
64.0 μl of Magnesium chloride (2.5 M)
480.0 μl of Polyethylene glycol 4,000 (50 w/v)
80.0 μl of tris chloride BCC pH 8.5 (1 M)
176.0 μl of water (100 v/v)
C7
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
100.0 μl of Calcium chloride (2 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C8
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 25 w/v -
pH 5.6
55.6 μl of Ammonium sulfate (3.6 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
344.4 μl of water (100 v/v)
C9
  • Lithium chloride 0.8 M -
  • Polyethylene glycol 4,000 32 w/v -
NA
80.0 μl of Lithium chloride (10 M)
640.0 μl of Polyethylene glycol 4,000 (50 w/v)
280.0 μl of water (100 v/v)
C10
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
C11
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
55.6 μl of Ammonium sulfate (3.6 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
244.4 μl of water (100 v/v)
C12
  • Calcium chloride 0.05 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
D1
  • Magnesium chloride 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
580.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 600 40 w/v -
pH 5.5
800.0 μl of Polyethylene glycol 600 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
100.0 μl of water (100 v/v)
D3
  • Lithium chloride 1 M -
  • Sodium acetate 0.1 M -
  • Polyethylene glycol 6,000 30 w/v -
NA
100.0 μl of Lithium chloride (10 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
50.0 μl of Sodium acetate (2 M)
250.0 μl of water (100 v/v)
D4
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
D5
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 8,000 20 w/v -
pH 6
200.0 μl of Calcium acetate (1 M)
33.3 μl of MES pH 6 (3 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
366.7 μl of water (100 v/v)
D6
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 20 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
D7
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 8,000 20 w/v -
pH 4.2
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
460.0 μl of water (100 v/v)
D8
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
55.6 μl of Ammonium sulfate (3.6 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
244.4 μl of water (100 v/v)
D9
  • Ammonium sulfate 0.05 M -
  • BIS-TRIS 0.05 M pH 6.5
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
pH 6.5
13.9 μl of Ammonium sulfate (3.6 M)
50.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
336.1 μl of water (100 v/v)
D10
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polypropylene glycol 400 28 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polypropylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D11
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polypropylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polypropylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
D12
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
  • Sodium chloride 2.5 M -
pH 7
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
320.0 μl of water (100 v/v)
E1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium chloride 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
60.0 μl of Ammonium chloride (5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
390.0 μl of water (100 v/v)
E2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium formate 0.3 M pH 6.6
  • Potassium nitrate 0.1 M -
pH 6.6
30.0 μl of Ammonium formate pH 6.6 (10 M)
33.3 μl of Potassium nitrate (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
486.7 μl of water (100 v/v)
E3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium nitrate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 5.9
pH 5.9
30.0 μl of Ammonium nitrate (10 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.9 (1 M)
420.0 μl of water (100 v/v)
E4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Ammonium di-hydrogen phosphate 0.3 M -
  • MES 0.1 M pH 5.7
pH 5.7
120.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of MES pH 5.7 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
330.0 μl of water (100 v/v)
E5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BIS-TRIS 0.1 M pH 6.8
  • Ammonium sulfate 0.3 M -
pH 6.8
85.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
364.3 μl of water (100 v/v)
E6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium acetate 0.1 M pH 5.8
  • di-Ammonium tartrate 0.3 M -
pH 5.8
150.0 μl of di-Ammonium tartrate (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
15.1 μl of Sodium acetate pH 5 (1 M)
84.9 μl of Sodium acetate pH 6.2 (1 M)
300.0 μl of water (100 v/v)
E7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Cadmium chloride 0.3 M -
  • Sodium acetate 0.1 M pH 3.7
pH 3.7
300.0 μl of Cadmium chloride (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 3.7 (1 M)
150.0 μl of water (100 v/v)
E8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Imidazole 0.1 M pH 4.6
  • Cadmium sulfate 0.3 M -
pH 4.6
300.0 μl of Cadmium sulfate (1 M)
97.0 μl of Imidazole pH 4.5 (1 M)
3.0 μl of Imidazole pH 5.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • HEPES 0.1 M pH 7.6
  • Cesium chloride 0.3 M -
pH 7.6
300.0 μl of Cesium chloride (1 M)
100.0 μl of HEPES pH 7.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BICINE 0.1 M pH 6.4
  • Calcium acetate 0.3 M -
pH 6.4
100.0 μl of BICINE pH 6.4 (1 M)
300.0 μl of Calcium acetate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
E11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BIS-TRIS propane 0.1 M pH 8.5
  • Calcium chloride 0.3 M -
pH 8.5
100.0 μl of BIS-TRIS propane pH 8.5 (1 M)
150.0 μl of Calcium chloride (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of water (100 v/v)
E12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium tartrate 0.1 M pH 3.2
  • Cobalt (II) chloride 0.3 M -
pH 3.2
300.0 μl of Cobalt (II) chloride (1 M)
33.3 μl of di-Ammonium tartrate pH 3.2 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
216.7 μl of water (100 v/v)
F1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium hydrogen citrate 0.3 M -
  • Magnesium formate 0.1 M -
NA
120.0 μl of di-Ammonium hydrogen citrate pH 4.9 (2.5 M)
100.0 μl of Magnesium formate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
330.0 μl of water (100 v/v)
F2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Sodium DL-malate 0.3 M pH 2.9
  • Sodium cacodylate 0.1 M -
pH 2.9
100.0 μl of di-Sodium DL-malate pH 2.9 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium cacodylate (1 M)
350.0 μl of water (100 v/v)
F3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Iron (III) chloride 0.3 M -
  • Potassium nitrate 0.1 M -
NA
300.0 μl of Iron (III) chloride (1 M)
33.3 μl of Potassium nitrate pH 1.1 (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
216.7 μl of water (100 v/v)
F4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Lithium chloride 0.3 M -
  • Potassium formate 0.1 M pH 6.3
pH 6.3
30.0 μl of Lithium chloride (10 M)
100.0 μl of Potassium formate pH 6.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
420.0 μl of water (100 v/v)
F5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • CHES 0.1 M pH 9.5
  • Lithium sulfate 0.3 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
150.0 μl of Lithium sulfate (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of water (100 v/v)
F6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium acetate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4.7
pH 4.7
300.0 μl of Magnesium acetate (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.7 (1 M)
150.0 μl of water (100 v/v)
F7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium cacodylate 0.1 M pH 5.8
  • Magnesium chloride 0.3 M -
pH 5.8
150.0 μl of Magnesium chloride (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium cacodylate pH 5.8 (1 M)
300.0 μl of water (100 v/v)
F8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • HEPES 0.1 M pH 7.4
  • Magnesium nitrate 0.3 M -
pH 7.4
100.0 μl of HEPES pH 7.4 (1 M)
100.0 μl of Magnesium nitrate (3 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
350.0 μl of water (100 v/v)
F9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Potassium formate 0.1 M pH 6.1
  • Magnesium sulfate 0.1 M -
pH 6.1
50.0 μl of Magnesium sulfate (2 M)
100.0 μl of Potassium formate pH 6.1 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
400.0 μl of water (100 v/v)
F10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • MES 0.1 M pH 5.7
  • Nickel (II) chloride 0.3 M -
pH 5.7
100.0 μl of MES pH 5.7 (1 M)
75.0 μl of Nickel (II) chloride (4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of water (100 v/v)
F11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BIS-TRIS 0.1 M pH 6.6
  • Potassium bromide 0.3 M -
pH 6.6
100.0 μl of BIS-TRIS pH 6.6 (1 M)
75.0 μl of Potassium bromide (4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of water (100 v/v)
F12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tris chloride BCC 0.1 M pH 6.1
  • Potassium di-hydrogen phosphate 0.3 M -
pH 6.1
200.0 μl of Potassium di-hydrogen phosphate (1.5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
24.6 μl of tris chloride BCC pH 6 (3 M)
8.7 μl of tris chloride BCC pH 6.3 (3 M)
316.7 μl of water (100 v/v)
G1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BICINE 0.1 M pH 8.8
  • Potassium Sodium tartrate 0.3 M -
pH 8.8
100.0 μl of BICINE pH 8.8 (1 M)
200.0 μl of Potassium Sodium tartrate (1.5 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
250.0 μl of water (100 v/v)
G2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Imidazole 0.1 M pH 7.3
  • di-Potassium tartrate 0.3 M -
pH 7.3
300.0 μl of di-Potassium tartrate (1 M)
100.0 μl of Imidazole pH 7.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
G3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Ammonium tartrate 0.1 M pH 5.8
pH 5.8
25.6 μl of di-Ammonium tartrate pH 5.5 (2 M)
24.4 μl of di-Ammonium tartrate pH 8.5 (2 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
500.0 μl of water (100 v/v)
G4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium bromide 0.3 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
60.0 μl of Sodium bromide (5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
390.0 μl of water (100 v/v)
G5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tris chloride BCC 0.1 M pH 8.1
  • Sodium chloride 0.3 M -
pH 8.1
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
60.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.1 (1 M)
390.0 μl of water (100 v/v)
G6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • CHES 0.1 M pH 9.5
  • Sodium fluoride 0.3 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
375.0 μl of Sodium fluoride (0.8 M)
75.0 μl of water (100 v/v)
G7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Sodium formate 0.3 M -
pH 4.5
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
42.9 μl of Sodium formate (7 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
407.1 μl of water (100 v/v)
G8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium acetate 0.1 M pH 4.7
  • Sodium iodide 0.3 M -
pH 4.7
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 4.7 (1 M)
300.0 μl of Sodium iodide (1 M)
150.0 μl of water (100 v/v)
G9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tri-Sodium citrate 0.1 M pH 6.8
  • di-Sodium malonate 0.3 M -
pH 6.8
88.2 μl of di-Sodium malonate pH 4 (3.4 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
5.2 μl of tri-Sodium citrate pH 5.8 (1 M)
94.8 μl of tri-Sodium citrate pH 7 (1 M)
361.8 μl of water (100 v/v)
G10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • MES 0.1 M pH 6.1
  • Sodium nitrate 0.3 M -
pH 6.1
100.0 μl of MES pH 6.1 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
42.9 μl of Sodium nitrate (7 M)
407.1 μl of water (100 v/v)
G11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BIS-TRIS 0.1 M pH 5.8
  • Sodium di-hydrogen phosphate 0.3 M -
pH 5.8
100.0 μl of BIS-TRIS pH 5.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
75.0 μl of Sodium di-hydrogen phosphate (4 M)
375.0 μl of water (100 v/v)
G12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BIS-TRIS propane 0.1 M pH 9.3
  • Sodium sulfate 0.3 M -
pH 9.3
100.0 μl of BIS-TRIS propane pH 9.3 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of Sodium sulfate (1 M)
150.0 μl of water (100 v/v)
H1
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • HEPES 0.1 M pH 7.8
  • Sodium thiocyanate 0.3 M -
pH 7.8
100.0 μl of HEPES pH 7.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
37.5 μl of Sodium thiocyanate (8 M)
412.5 μl of water (100 v/v)
H2
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • di-Sodium succinate 0.3 M -
  • Imidazole 0.1 M -
NA
300.0 μl of di-Sodium succinate pH 3.4 (1 M)
100.0 μl of Imidazole (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of water (100 v/v)
H3
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • BICINE 0.1 M pH 8.6
  • Trimethylamine N-oxide 0.3 M -
pH 8.6
100.0 μl of BICINE pH 8.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
300.0 μl of Trimethylamine N-oxide (1 M)
150.0 μl of water (100 v/v)
H4
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tri-Potassium citrate 0.3 M -
  • BIS-TRIS propane 0.1 M pH 9.8
pH 9.8
100.0 μl of BIS-TRIS propane pH 9.8 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
120.0 μl of tri-Potassium citrate (2.5 M)
330.0 μl of water (100 v/v)
H5
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • HEPES 0.1 M pH 7.5
  • Jeffamine® M-600 10 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
250.0 μl of water (100 v/v)
H6
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium formate 0.1 M pH 5.7
  • Zinc chloride 0.3 M -
pH 5.7
100.0 μl of Magnesium formate pH 5.7 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
150.0 μl of Zinc chloride (2 M)
300.0 μl of water (100 v/v)
H7
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Zinc sulfate 0.3 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
150.0 μl of Zinc sulfate (2 M)
300.0 μl of water (100 v/v)
H8
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • CHES 0.1 M pH 9.9
pH 9.9
47.4 μl of CHES pH 9.5 (1 M)
52.6 μl of CHES pH 10.5 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H9
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Imidazole 0.1 M pH 6.6
pH 6.6
100.0 μl of Imidazole pH 6.6 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H10
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Magnesium formate 0.1 M pH 5.9
pH 5.9
100.0 μl of Magnesium formate pH 5.9 (1 M)
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
450.0 μl of water (100 v/v)
H11
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • Sodium acetate 0.1 M pH 4.7
pH 4.7
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of Sodium acetate pH 4.7 (1 M)
450.0 μl of water (100 v/v)
H12
  • PurePEGs 0.3-8 kD 22.5 w/v -
  • tris chloride BCC 0.1 M pH 8.1
pH 8.1
450.0 μl of PurePEGs 0.3-8 kD (50 w/v)
100.0 μl of tris chloride BCC pH 8.1 (1 M)
450.0 μl of water (100 v/v)