C6 - Screen Contents

Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Jump to well contents.

Supplemental Information

Screen Description

SuperSMART

Microlytic-Anatrace / SuperSMART

Created 2013-03-25.

Export

SuperSMART

Barcodes

SuperSMART

No barcodes found.

Screen Structure

SuperSMART

Well Count	48
Number of distinct ingredients	41
Internal diversity (score mean dist)	0.939

Screen Composition

More information about screen composition chart.

ingredient type	SuperSMART
Buffer	47
Cryoprotectant	3
Organic (non-volatile)	10
Polymer	42
Salt	51

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

SuperSMART Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium chloride 0.1 M - tris chloride BCC 0.1 M pH 6.5 Ammonium sulfate 1.5 M - 🔍	pH 6.5	416.7 μl of Ammonium sulfate (3.6 M) 20.0 μl of Sodium chloride (5 M) 28.2 μl of tris chloride BCC pH 6.3 (3 M) 5.1 μl of tris chloride BCC pH 7 (3 M) 530.0 μl of water (100 v/v)
A2	Ammonium di-hydrogen phosphate 0.2 M - tris chloride BCC 0.1 M pH 8.5 2-methyl-2,4-pentanediol BCC 50 v/v - 🔍	pH 8.5	500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
A3	Calcium chloride 0.2 M - BIS-TRIS 0.1 M pH 5.5 2-methyl-2,4-pentanediol BCC 45 v/v - 🔍	pH 5.5	450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Calcium chloride (2 M) 350.0 μl of water (100 v/v)
A4	Sodium chloride 0.2 M - Sodium Potassium phosphate 0.1 M pH 6.2 Polyethylene glycol 1,000 20 w/v - 🔍	pH 6.2	400.0 μl of Polyethylene glycol 1,000 (50 w/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40.0 μl of Sodium chloride (5 M) 526.7 μl of water (100 v/v)
A5	BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 2,000 28 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 340.0 μl of water (100 v/v)
A6	Potassium thiocyanate 0.1 M - Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v)
A7	Potassium bromide 0.15 M - Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	NA	600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v)
A8	Polyethylene glycol monomethyl ether 2,000 25 w/v - 🔍	NA	500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 500.0 μl of water (100 v/v)
B1	Sodium acetate 0.1 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 2,000 30 w/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 250.0 μl of water (100 v/v)
B2	Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 300 40 v/v - 🔍	pH 4.2	400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 500.0 μl of water (100 v/v)
B3	Calcium acetate 0.1 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 300 40 v/v - 🔍	pH 6.5	100.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v)
B4	Ammonium sulfate 0.2 M - BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 5.5	55.6 μl of Ammonium sulfate (3.6 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 344.4 μl of water (100 v/v)
B5	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v)
B6	Sodium chloride 0.2 M - BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 5.5	100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v)
B7	Ammonium acetate 0.2 M - BIS-TRIS 0.1 M pH 5.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 5.5	200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v)
B8	Magnesium chloride 0.2 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 80.0 μl of Magnesium chloride pH 7 (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v)
C1	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride pH 7 (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
C2	Sodium thiocyanate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate pH 6.9 (8 M) 575.0 μl of water (100 v/v)
C3	Potassium thiocyanate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Potassium thiocyanate (8 M) 575.0 μl of water (100 v/v)
C4	Potassium Sodium tartrate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of Potassium Sodium tartrate (1.5 M) 466.7 μl of water (100 v/v)
C5	tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 3,350 25 w/v - 🔍	pH 8.5	500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
C6	Potassium iodide 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Potassium iodide (1 M) 400.0 μl of water (100 v/v)
C7	Lithium acetate 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	40.0 μl of Lithium acetate (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v)
C8	Potassium chloride 0.2 M - Polyethylene glycol 3,350 20 w/v - 🔍	NA	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Potassium chloride (4 M) 550.0 μl of water (100 v/v)
D1	Lithium sulfate 0.2 M - HEPES 0.1 M pH 7.5 Polyethylene glycol 3,350 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
D2	Magnesium chloride 0.1 M - MES 0.1 M pH 6.5 Polyethylene glycol 400 30 v/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 40.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 560.0 μl of water (100 v/v)
D3	CAPS 0.1 M pH 10 Polyethylene glycol 400 30 v/v - 🔍	pH 10	100.0 μl of CAPS pH 10 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 600.0 μl of water (100 v/v)
D4	Ammonium acetate 0.17 M - tri-Sodium citrate 0.085 M pH 5.6 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 5.6	170.0 μl of Ammonium acetate (1 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of tri-Sodium citrate pH 5.6 (1 M) 85.0 μl of water (100 v/v)
D5	Sodium acetate 0.17 M - tris chloride BCC 0.085 M pH 8.5 Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - 🔍	pH 8.5	150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 85.0 μl of Sodium acetate (2 M) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 170.0 μl of water (100 v/v)
D6	Magnesium chloride 0.16 M - tris chloride BCC 0.08 M pH 8.5 Polyethylene glycol 4,000 24 w/v - Glycerol 20 v/v - 🔍	pH 8.5	200.0 μl of Glycerol (100 v/v) 64.0 μl of Magnesium chloride (2.5 M) 480.0 μl of Polyethylene glycol 4,000 (50 w/v) 80.0 μl of tris chloride BCC pH 8.5 (1 M) 176.0 μl of water (100 v/v)
D7	Calcium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 4,000 25 w/v - 🔍	pH 8.5	100.0 μl of Calcium chloride (2 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
D8	Ammonium sulfate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 Polyethylene glycol 4,000 25 w/v - 🔍	pH 5.6	55.6 μl of Ammonium sulfate (3.6 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 344.4 μl of water (100 v/v)
E1	Lithium chloride 0.8 M - Polyethylene glycol 4,000 32 w/v - 🔍	NA	80.0 μl of Lithium chloride (10 M) 640.0 μl of Polyethylene glycol 4,000 (50 w/v) 280.0 μl of water (100 v/v)
E2	BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 20 w/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v)
E3	Ammonium sulfate 0.2 M - MES 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 5,000 30 w/v - 🔍	pH 6.5	55.6 μl of Ammonium sulfate (3.6 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 244.4 μl of water (100 v/v)
E4	Calcium chloride 0.05 M - BIS-TRIS 0.1 M pH 6.5 Polyethylene glycol monomethyl ether 550 30 v/v - 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 25.0 μl of Calcium chloride (2 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 575.0 μl of water (100 v/v)
E5	Magnesium chloride 0.05 M - HEPES 0.1 M pH 7.5 Polyethylene glycol monomethyl ether 550 30 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 580.0 μl of water (100 v/v)
E6	tri-Sodium citrate 0.1 M pH 5.5 Polyethylene glycol 600 40 w/v - 🔍	pH 5.5	800.0 μl of Polyethylene glycol 600 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 100.0 μl of water (100 v/v)
E7	Lithium chloride 1 M - Sodium acetate 0.1 M - Polyethylene glycol 6,000 30 w/v - 🔍	NA	100.0 μl of Lithium chloride (10 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 50.0 μl of Sodium acetate (2 M) 250.0 μl of water (100 v/v)
E8	HEPES 0.1 M pH 7.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v)
F1	Calcium acetate 0.2 M - MES 0.1 M pH 6 Polyethylene glycol 8,000 20 w/v - 🔍	pH 6	200.0 μl of Calcium acetate (1 M) 33.3 μl of MES pH 6 (3 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 366.7 μl of water (100 v/v)
F2	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 Polyethylene glycol 8,000 20 w/v - 🔍	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v)
F3	Sodium chloride 0.2 M - Sodium phosphate citrate 0.1 M pH 4.2 Polyethylene glycol 8,000 20 w/v - 🔍	pH 4.2	400.0 μl of Polyethylene glycol 8,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 460.0 μl of water (100 v/v)
F4	Ammonium sulfate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 Polyethylene glycol 8,000 30 w/v - 🔍	pH 6.5	55.6 μl of Ammonium sulfate (3.6 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 244.4 μl of water (100 v/v)
F5	Ammonium sulfate 0.05 M - BIS-TRIS 0.05 M pH 6.5 Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v - 🔍	pH 6.5	13.9 μl of Ammonium sulfate (3.6 M) 50.0 μl of BIS-TRIS pH 6.5 (1 M) 600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v) 336.1 μl of water (100 v/v)
F6	Calcium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polypropylene glycol 400 28 v/v - 🔍	pH 7.5	100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polypropylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v)
F7	Magnesium chloride 0.2 M - HEPES 0.1 M pH 7.5 Polypropylene glycol 400 30 v/v - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polypropylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v)
F8	Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 7 Sodium chloride 2.5 M - 🔍	pH 7	80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 320.0 μl of water (100 v/v)