Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Jump to well contents.

Supplemental Information

Screen Description

MemMeso

Created 2020-05-11.

Export

MemMeso

Barcodes

MemMeso

    Screen Structure

    MemMeso

    Well Count 96
    Number of distinct ingredients 16
    Internal diversity (score mean dist) 0.823

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:36:47.766434 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemMeso
    Buffer 96
    Organic (non-volatile) 32
    Polymer 64
    Salt 164

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemMeso Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 40 v/v -
    		pH 5
    50 μl of Magnesium chloride (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    487 μl of water (100 v/v)
    A2
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 40 v/v -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    50 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    467 μl of water (100 v/v)
    A3
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 40 v/v -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    517 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 200 40 v/v -
    		pH 5
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    537 μl of water (100 v/v)
    A5
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 40 v/v -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    330 μl of water (100 v/v)
    A6
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 40 v/v -
    • Lithium sulfate 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    330 μl of water (100 v/v)
    A7
    • Sodium chloride 0.1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 40 v/v -
    • Calcium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    330 μl of water (100 v/v)
    A8
    • Zinc acetate 0.01 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 200 40 v/v -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    10 μl of Zinc acetate (1 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    390 μl of water (100 v/v)
    A9
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 200 40 v/v -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    430 μl of water (100 v/v)
    A10
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 200 40 v/v -
    		pH 7
    50 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    450 μl of water (100 v/v)
    A11
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 200 40 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    430 μl of water (100 v/v)
    A12
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 200 40 v/v -
    		pH 7
    400 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    500 μl of water (100 v/v)
    B1
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 200 40 v/v -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    430 μl of water (100 v/v)
    B2
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 200 40 v/v -
    • Lithium sulfate 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    430 μl of water (100 v/v)
    B3
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 200 40 v/v -
    • Calcium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    430 μl of water (100 v/v)
    B4
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 200 40 v/v -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    442 μl of water (100 v/v)
    B5
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • Polyethylene glycol 300 30 v/v -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    B6
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 300 30 v/v -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    50 μl of Lithium sulfate (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    B7
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 300 30 v/v -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    617 μl of water (100 v/v)
    B8
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 300 30 v/v -
    		pH 5
    300 μl of Polyethylene glycol 300 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    637 μl of water (100 v/v)
    B9
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    B10
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    B11
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Calcium chloride (2 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    B12
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 30 v/v -
    • Zinc acetate 0.01 M -
    		pH 6
    300 μl of Polyethylene glycol 300 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    10 μl of Zinc acetate (1 M)
    83 μl of MES pH 7.1 (0.5 M)
    490 μl of water (100 v/v)
    C1
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    C2
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    C3
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    C4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 300 30 v/v -
    		pH 7
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    600 μl of water (100 v/v)
    C5
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 30 v/v -
    		pH 8
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    550 μl of water (100 v/v)
    C6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 30 v/v -
    • Lithium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    530 μl of water (100 v/v)
    C7
    • Polyethylene glycol 300 30 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    C8
    • Polyethylene glycol 300 30 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.2 M -
    		pH 8
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    542 μl of water (100 v/v)
    C9
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    567 μl of water (100 v/v)
    C10
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    50 μl of Lithium sulfate (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    567 μl of water (100 v/v)
    C11
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    617 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 30 v/v -
    		pH 5
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    637 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    430 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6
    • Lithium sulfate 0.1 M -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Lithium sulfate (2 M)
    83 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6
    • Calcium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    430 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6
    • Zinc acetate 0.01 M -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    10 μl of Zinc acetate (1 M)
    83 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    490 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    530 μl of water (100 v/v)
    D6
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    530 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    530 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    		pH 7
    100 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    530 μl of water (100 v/v)
    D10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    530 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    • Calcium chloride 0.1 M -
    		pH 8
    50 μl of Calcium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    D12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    • Ammonium sulfate 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    542 μl of water (100 v/v)
    E1
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Magnesium chloride 0.1 M -
    		pH 5
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    587 μl of water (100 v/v)
    E2
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    50 μl of Lithium sulfate (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    E3
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    617 μl of water (100 v/v)
    E4
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    		pH 5
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    637 μl of water (100 v/v)
    E5
    • MES 0.1 M pH 6
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    E6
    • MES 0.1 M pH 6
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Lithium sulfate (2 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    E7
    • MES 0.1 M pH 6
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    E8
    • MES 0.1 M pH 6
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Zinc acetate 0.01 M -
    		pH 6
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    10 μl of Zinc acetate (1 M)
    83 μl of MES pH 7.1 (0.5 M)
    490 μl of water (100 v/v)
    E9
    • HEPES 0.1 M pH 7
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 7
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Lithium sulfate 0.1 M -
    		pH 7
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7 (1 M)
    550 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    E12
    • HEPES 0.1 M pH 7
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    		pH 7
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    600 μl of water (100 v/v)
    F1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    F2
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    530 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    • Ammonium sulfate 0.2 M -
    		pH 8
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    542 μl of water (100 v/v)
    F5
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    F6
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    50 μl of Lithium sulfate (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    F7
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    617 μl of water (100 v/v)
    F8
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    		pH 5
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    637 μl of water (100 v/v)
    F9
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    F10
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    50 μl of Lithium sulfate (2 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    F11
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Calcium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    F12
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Zinc acetate 0.01 M -
    		pH 6
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    117 μl of MES pH 5.2 (0.5 M)
    10 μl of Zinc acetate (1 M)
    83 μl of MES pH 7.1 (0.5 M)
    490 μl of water (100 v/v)
    G1
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    G2
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    G3
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    G4
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    		pH 7
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    600 μl of water (100 v/v)
    G5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Magnesium chloride 0.1 M -
    		pH 8
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    550 μl of water (100 v/v)
    G6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    530 μl of water (100 v/v)
    G7
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    50 μl of Calcium chloride (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    G8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 500 30 v/v -
    • Ammonium sulfate 0.2 M -
    		pH 8
    300 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    542 μl of water (100 v/v)
    G9
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    G10
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    567 μl of water (100 v/v)
    G11
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    		pH 5
    20 μl of Sodium chloride (5 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    617 μl of water (100 v/v)
    G12
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 600 30 v/v -
    		pH 5
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    637 μl of water (100 v/v)
    H1
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    H2
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 v/v -
    • Lithium sulfate 0.1 M -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    83 μl of MES pH 7.1 (0.5 M)
    450 μl of water (100 v/v)
    H3
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 v/v -
    • Calcium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    		pH 6
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    83 μl of MES pH 7.1 (0.5 M)
    430 μl of water (100 v/v)
    H4
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 v/v -
    • Zinc acetate 0.01 M -
    		pH 6
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    10 μl of Zinc acetate (1 M)
    83 μl of MES pH 7.1 (0.5 M)
    490 μl of water (100 v/v)
    H5
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    H6
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    50 μl of Lithium sulfate (2 M)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    H7
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.1 M -
    		pH 7
    20 μl of Sodium chloride (5 M)
    50 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    530 μl of water (100 v/v)
    H8
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 600 30 v/v -
    		pH 7
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    600 μl of water (100 v/v)
    H9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    H10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 30 v/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    		pH 8
    20 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    530 μl of water (100 v/v)
    H11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 30 v/v -
    • Calcium chloride 0.1 M -
    		pH 8
    50 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    550 μl of water (100 v/v)
    H12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 30 v/v -
    • Ammonium sulfate 0.2 M -
    		pH 8
    300 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    542 μl of water (100 v/v)