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Supplemental Information

Screen Description

MemMeso

Created 2020-05-11.

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MemMeso

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Screen Structure

MemMeso

Well Count 96
Number of distinct ingredients 16
Internal diversity (score mean dist) 0.823

Screen Composition

2025-08-16T14:27:58.887842 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemMeso
Buffer 96
Organic (non-volatile) 32
Polymer 64
Salt 164

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemMeso Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 200 40 v/v -
pH 5
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
487.5 μl of water (100 v/v)
A2
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 200 40 v/v -
pH 5
50.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
467.5 μl of water (100 v/v)
A3
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 200 40 v/v -
pH 5
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
517.5 μl of water (100 v/v)
A4
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 200 40 v/v -
pH 5
400.0 μl of Polyethylene glycol 200 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
537.5 μl of water (100 v/v)
A5
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 200 40 v/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
330.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 200 40 v/v -
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
330.0 μl of water (100 v/v)
A7
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 200 40 v/v -
  • Calcium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
330.0 μl of water (100 v/v)
A8
  • Zinc acetate 0.01 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 200 40 v/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
10.0 μl of Zinc acetate (1 M)
390.0 μl of water (100 v/v)
A9
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 200 40 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
A10
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 200 40 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
450.0 μl of water (100 v/v)
A11
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 200 40 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
A12
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 200 40 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
500.0 μl of water (100 v/v)
B1
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
  • Polyethylene glycol 200 40 v/v -
pH 8
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
430.0 μl of water (100 v/v)
B2
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 200 40 v/v -
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
430.0 μl of water (100 v/v)
B3
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 200 40 v/v -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
430.0 μl of water (100 v/v)
B4
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 200 40 v/v -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
442.9 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
  • Polyethylene glycol 300 30 v/v -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
B6
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • Polyethylene glycol 300 30 v/v -
pH 5
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 300 30 v/v -
pH 5
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
B8
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 300 30 v/v -
pH 5
300.0 μl of Polyethylene glycol 300 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
B9
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
B10
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
B11
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
B12
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 30 v/v -
  • Zinc acetate 0.01 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
10.0 μl of Zinc acetate (1 M)
490.0 μl of water (100 v/v)
C1
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
C2
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
C3
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
C4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 300 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
600.0 μl of water (100 v/v)
C5
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 300 30 v/v -
pH 8
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
C6
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 300 30 v/v -
  • Lithium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 300 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 300 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 0.2 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
542.9 μl of water (100 v/v)
C9
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
C10
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 5
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
C11
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
pH 5
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 30 v/v -
pH 5
300.0 μl of Polyethylene glycol 400 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
D1
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6
  • Calcium chloride 0.1 M -
  • Sodium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6
  • Zinc acetate 0.01 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
10.0 μl of Zinc acetate (1 M)
490.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
D9
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 8
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
D10
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
D12
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
  • Ammonium sulfate 0.2 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
542.9 μl of water (100 v/v)
E1
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Magnesium chloride 0.1 M -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
587.5 μl of water (100 v/v)
E2
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 5
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
E3
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium chloride 0.1 M -
pH 5
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
E4
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
E5
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
E6
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
E7
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
E8
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Zinc acetate 0.01 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
10.0 μl of Zinc acetate (1 M)
490.0 μl of water (100 v/v)
E9
  • HEPES 0.1 M pH 7
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 7
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Lithium sulfate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
550.0 μl of water (100 v/v)
E11
  • HEPES 0.1 M pH 7
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
E12
  • HEPES 0.1 M pH 7
  • Polyethylene glycol dimethyl ether 500 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
600.0 μl of water (100 v/v)
F1
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 8
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
F2
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
F3
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
F4
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol dimethyl ether 500 30 v/v -
  • Ammonium sulfate 0.2 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
542.9 μl of water (100 v/v)
F5
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
F6
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 5
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
F7
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
pH 5
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
F8
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 500 30 v/v -
pH 5
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
F9
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
F10
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
F11
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
F12
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Zinc acetate 0.01 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
10.0 μl of Zinc acetate (1 M)
490.0 μl of water (100 v/v)
G1
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
G2
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
G3
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
G4
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 500 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
600.0 μl of water (100 v/v)
G5
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Magnesium chloride 0.1 M -
pH 8
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
G6
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
G7
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
G8
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 500 30 v/v -
  • Ammonium sulfate 0.2 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
542.9 μl of water (100 v/v)
G9
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 5
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
G10
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 5
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
567.5 μl of water (100 v/v)
G11
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
pH 5
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
617.5 μl of water (100 v/v)
G12
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 600 30 v/v -
pH 5
300.0 μl of Polyethylene glycol 600 (100 v/v)
29.9 μl of tri-Sodium citrate pH 3 (1.6 M)
32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M)
637.5 μl of water (100 v/v)
H1
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
H2
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 v/v -
  • Lithium sulfate 0.1 M -
pH 6
50.0 μl of Lithium sulfate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
450.0 μl of water (100 v/v)
H3
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 v/v -
  • Calcium chloride 0.1 M -
  • Sodium chloride 0.1 M -
pH 6
50.0 μl of Calcium chloride (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
H4
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 v/v -
  • Zinc acetate 0.01 M -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
10.0 μl of Zinc acetate (1 M)
490.0 μl of water (100 v/v)
H5
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
H6
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
H7
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.1 M -
pH 7
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
H8
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 600 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
600.0 μl of water (100 v/v)
H9
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
pH 8
50.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
H10
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 600 30 v/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
H11
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 600 30 v/v -
  • Calcium chloride 0.1 M -
pH 8
50.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
550.0 μl of water (100 v/v)
H12
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 600 30 v/v -
  • Ammonium sulfate 0.2 M -
pH 8
57.1 μl of Ammonium sulfate (3.5 M)
300.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
542.9 μl of water (100 v/v)