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Supplemental Information

Screen Description

Helix

Created 2014-10-14.

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Helix

    Screen Structure

    Helix

    Well Count 96
    Number of distinct ingredients 33
    Internal diversity (score mean dist) 0.874

    Screen Composition

    2025-06-13T21:40:03.752763 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Helix
    Buffer 132
    Cryoprotectant 6
    Organic (non-volatile) 45
    Polymer 52
    Salt 145

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Helix Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Potassium chloride 0.05 M -
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 400 25 v/v -
    • Spermine 0.012 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Lithium chloride (10 M)
    120 μl of Spermine (0.1 M)
    12 μl of Potassium chloride (4 M)
    507 μl of water (100 v/v)
    A2
    • Polyethylene glycol 400 25 v/v -
    • Spermine 0.001 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Spermine (0.1 M)
    640 μl of water (100 v/v)
    A3
    • Sodium chloride 0.1 M -
    • Lithium chloride 0.05 M -
    • Polyethylene glycol 400 25 v/v -
    • Magnesium chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    5 μl of Lithium chloride (10 M)
    20 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    620 μl of water (100 v/v)
    A4
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 400 25 v/v -
    • Cobalt (III) Hexamine chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Cobalt (III) Hexamine chloride (0.1 M)
    50 μl of Potassium chloride (4 M)
    550 μl of water (100 v/v)
    A5
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 400 25 v/v -
    • Manganese (II) chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Lithium chloride (10 M)
    10 μl of Manganese (II) chloride (1 M)
    630 μl of water (100 v/v)
    A6
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 400 25 v/v -
    • Strontium chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    50 μl of Strontium chloride (0.1 M)
    580 μl of water (100 v/v)
    A7
    • Potassium chloride 0.05 M -
    • Lithium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Spermine 0.012 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    120 μl of Spermine (0.1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    12 μl of Potassium chloride (4 M)
    407 μl of water (100 v/v)
    A8
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Spermine 0.001 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Spermine (0.1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    540 μl of water (100 v/v)
    A9
    • Sodium chloride 0.1 M -
    • Lithium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Magnesium chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    20 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    515 μl of water (100 v/v)
    A10
    • Potassium chloride 0.2 M -
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Cobalt (III) Hexamine chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Cobalt (III) Hexamine chloride (0.1 M)
    50 μl of Potassium chloride (4 M)
    450 μl of water (100 v/v)
    A11
    • Lithium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Manganese (II) chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Manganese (II) chloride (1 M)
    530 μl of water (100 v/v)
    A12
    • Sodium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Strontium chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    20 μl of Sodium chloride (5 M)
    50 μl of Strontium chloride (0.1 M)
    480 μl of water (100 v/v)
    B1
    • Potassium chloride 0.05 M -
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 4,000 17 w/v -
    • Spermine 0.012 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    120 μl of Spermine (0.1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    12 μl of Potassium chloride (4 M)
    417 μl of water (100 v/v)
    B2
    • Polyethylene glycol 4,000 17 w/v -
    • Spermine 0.001 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Spermine (0.1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    550 μl of water (100 v/v)
    B3
    • Sodium chloride 0.1 M -
    • Lithium chloride 0.05 M -
    • Polyethylene glycol 4,000 17 w/v -
    • Magnesium chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    5 μl of Lithium chloride (10 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    530 μl of water (100 v/v)
    B4
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 4,000 17 w/v -
    • Cobalt (III) Hexamine chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Cobalt (III) Hexamine chloride (0.1 M)
    50 μl of Potassium chloride (4 M)
    460 μl of water (100 v/v)
    B5
    • Lithium chloride 0.1 M -
    • Polyethylene glycol 4,000 17 w/v -
    • Manganese (II) chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    10 μl of Manganese (II) chloride (1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    540 μl of water (100 v/v)
    B6
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 4,000 17 w/v -
    • Strontium chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    50 μl of Strontium chloride (0.1 M)
    490 μl of water (100 v/v)
    B7
    • Potassium chloride 0.05 M -
    • Lithium chloride 0.1 M -
    • di-Sodium malonate 2.6 M -
    • Spermine 0.012 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    120 μl of Spermine (0.1 M)
    650 μl of di-Sodium malonate (4 M)
    12 μl of Potassium chloride (4 M)
    107 μl of water (100 v/v)
    B8
    • di-Sodium malonate 2.6 M -
    • Spermine 0.001 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Spermine (0.1 M)
    650 μl of di-Sodium malonate (4 M)
    240 μl of water (100 v/v)
    B9
    • Sodium chloride 0.1 M -
    • Lithium chloride 0.05 M -
    • di-Sodium malonate 2.6 M -
    • Magnesium chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    5 μl of Lithium chloride (10 M)
    650 μl of di-Sodium malonate (4 M)
    20 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    220 μl of water (100 v/v)
    B10
    • Potassium chloride 0.2 M -
    • di-Sodium malonate 2.6 M -
    • Cobalt (III) Hexamine chloride 0.005 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    650 μl of di-Sodium malonate (4 M)
    50 μl of Cobalt (III) Hexamine chloride (0.1 M)
    50 μl of Potassium chloride (4 M)
    150 μl of water (100 v/v)
    B11
    • Lithium chloride 0.1 M -
    • di-Sodium malonate 2.6 M -
    • Manganese (II) chloride 0.01 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    10 μl of Lithium chloride (10 M)
    10 μl of Manganese (II) chloride (1 M)
    650 μl of di-Sodium malonate (4 M)
    230 μl of water (100 v/v)
    B12
    • Sodium chloride 0.1 M -
    • di-Sodium malonate 2.6 M -
    • Strontium chloride 0.002 M -
    • MES 0.05 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (0.5 M)
    650 μl of di-Sodium malonate (4 M)
    20 μl of Sodium chloride (5 M)
    20 μl of Strontium chloride (0.1 M)
    210 μl of water (100 v/v)
    C1
    • Ammonium sulfate 0.1 M -
    • Polyethylene glycol 200 44 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    440 μl of Polyethylene glycol 200 (100 v/v)
    481 μl of water (100 v/v)
    C2
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 400 40 v/v -
    • Calcium chloride 0.01 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    400 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    5 μl of Calcium chloride (2 M)
    50 μl of Potassium chloride (4 M)
    495 μl of water (100 v/v)
    C3
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 1,000 37 w/v -
    • Spermine 0.002 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    20 μl of Spermine (0.1 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    740 μl of Polyethylene glycol 1,000 (50 w/v)
    170 μl of water (100 v/v)
    C4
    • Sodium chloride 0.1 M -
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 2,000 35 w/v -
    • Magnesium chloride 0.02 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    700 μl of Polyethylene glycol 2,000 (50 w/v)
    10 μl of Magnesium chloride (2 M)
    50 μl of Potassium chloride (4 M)
    170 μl of water (100 v/v)
    C5
    • Potassium chloride 0.05 M -
    • Polyethylene glycol 3,350 33 w/v -
    • Spermine 0.005 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of Spermine (0.1 M)
    660 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    12 μl of Potassium chloride (4 M)
    227 μl of water (100 v/v)
    C6
    • Ammonium sulfate 0.1 M -
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 8,000 20 w/v -
    • Sodium formate 1 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    20 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    142 μl of Sodium formate (7 M)
    358 μl of water (100 v/v)
    C7
    • Polyethylene glycol 20,000 20 w/v -
    • Sodium formate 1 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    666 μl of Polyethylene glycol 20,000 (30 w/v)
    142 μl of Sodium formate (7 M)
    140 μl of water (100 v/v)
    C8
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol monomethyl ether 500 40 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    25 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    525 μl of water (100 v/v)
    C9
    • tri-Sodium citrate 0.1 M -
    • Polyethylene glycol monomethyl ether 500 40 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    62 μl of tri-Sodium citrate (1.6 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    487 μl of water (100 v/v)
    C10
    • Ammonium sulfate 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Spermine 0.002 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    20 μl of Spermine (0.1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    28 μl of Ammonium sulfate (3.5 M)
    501 μl of water (100 v/v)
    C11
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    600 μl of water (100 v/v)
    C12
    • Sodium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Magnesium chloride 0.02 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    10 μl of Magnesium chloride (2 M)
    620 μl of water (100 v/v)
    D1
    • Potassium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 27 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    270 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    655 μl of water (100 v/v)
    D2
    • 2-methyl-2,4-pentanediol BCC 24 v/v -
    • Polyethylene glycol 400 5 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of Polyethylene glycol 400 (100 v/v)
    240 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    660 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.2 M -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    100 μl of Lithium sulfate (2 M)
    650 μl of water (100 v/v)
    D4
    • Polyethylene glycol 3,350 5 w/v -
    • Ammonium sulfate 1 M -
    • Glycerol 20 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    100 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    200 μl of Glycerol (100 v/v)
    364 μl of water (100 v/v)
    D5
    • Polyethylene glycol monomethyl ether 500 5 v/v -
    • Ammonium sulfate 1.2 M -
    • Glycerol 10 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    342 μl of Ammonium sulfate (3.5 M)
    50 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    100 μl of Glycerol (100 v/v)
    457 μl of water (100 v/v)
    D6
    • Ammonium sulfate 1.4 M -
    • Glycerol 25 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    400 μl of Ammonium sulfate (3.5 M)
    250 μl of Glycerol (100 v/v)
    300 μl of water (100 v/v)
    D7
    • Potassium chloride 0.15 M -
    • Ammonium sulfate 1.6 M -
    • Glycerol 25 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    250 μl of Glycerol (100 v/v)
    37 μl of Potassium chloride (4 M)
    205 μl of water (100 v/v)
    D8
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 1.8 M -
    • Glycerol 25 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    20 μl of Sodium chloride (5 M)
    250 μl of Glycerol (100 v/v)
    165 μl of water (100 v/v)
    D9
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 5 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    D10
    • Potassium chloride 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 22 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    440 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    485 μl of water (100 v/v)
    D11
    • Lithium chloride 0.2 M -
    • Polyethylene glycol monomethyl ether 2,000 22 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    20 μl of Lithium chloride (10 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    440 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    490 μl of water (100 v/v)
    D12
    • Sodium chloride 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 22 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    440 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    490 μl of water (100 v/v)
    E1
    • Potassium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    25 μl of Potassium chloride (4 M)
    725 μl of water (100 v/v)
    E2
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • Spermine 0.005 M -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of Spermine (0.1 M)
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    800 μl of water (100 v/v)
    E3
    • Sodium chloride 0.1 M -
    • Potassium chloride 0.2 M -
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    50 μl of Potassium chloride (4 M)
    780 μl of water (100 v/v)
    E4
    • Sodium chloride 0.2 M -
    • 2-methyl-2,4-pentanediol BCC 25 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    660 μl of water (100 v/v)
    E5
    • Lithium sulfate 0.05 M -
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    25 μl of Lithium sulfate (2 M)
    775 μl of water (100 v/v)
    E6
    • Sodium chloride 0.3 M -
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    60 μl of Sodium chloride (5 M)
    590 μl of water (100 v/v)
    E7
    • Sodium chloride 0.05 M -
    • Polyethylene glycol 400 25 v/v -
    • Magnesium chloride 0.01 M -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of HEPES pH 6.5 (1 M)
    10 μl of Sodium chloride (5 M)
    5 μl of Magnesium chloride (2 M)
    685 μl of water (100 v/v)
    E8
    • Potassium chloride 0.2 M -
    • Polyethylene glycol 400 10 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    100 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    50 μl of Potassium chloride (4 M)
    800 μl of water (100 v/v)
    E9
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol 1,000 18 w/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    25 μl of Lithium sulfate (2 M)
    360 μl of Polyethylene glycol 1,000 (50 w/v)
    565 μl of water (100 v/v)
    E10
    • Lithium sulfate 1.5 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    750 μl of Lithium sulfate (2 M)
    200 μl of water (100 v/v)
    E11
    • Sodium chloride 0.1 M -
    • Potassium chloride 0.1 M -
    • Lithium sulfate 1.9 M -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    633 μl of Lithium sulfate (3 M)
    50 μl of HEPES pH 6.5 (1 M)
    20 μl of Sodium chloride (5 M)
    25 μl of Potassium chloride (4 M)
    271 μl of water (100 v/v)
    E12
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • Magnesium chloride 0.02 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    10 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    640 μl of water (100 v/v)
    F1
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol monomethyl ether 2,000 10 w/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    25 μl of Lithium sulfate (2 M)
    200 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    725 μl of water (100 v/v)
    F2
    • Polyethylene glycol monomethyl ether 2,000 14 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    280 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    670 μl of water (100 v/v)
    F3
    • Lithium chloride 0.1 M -
    • Polyethylene glycol monomethyl ether 500 10 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    10 μl of Lithium chloride (10 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    100 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
    840 μl of water (100 v/v)
    F4
    • Potassium chloride 0.05 M -
    • Ammonium sulfate 1.1 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    314 μl of Ammonium sulfate (3.5 M)
    12 μl of Potassium chloride (4 M)
    623 μl of water (100 v/v)
    F5
    • Lithium sulfate 0.05 M -
    • Ammonium sulfate 1.4 M -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    400 μl of Ammonium sulfate (3.5 M)
    25 μl of Lithium sulfate (2 M)
    525 μl of water (100 v/v)
    F6
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 1.8 M -
    • Lithium chloride 0.01 M -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    1 μl of Lithium chloride (10 M)
    50 μl of HEPES pH 6.5 (1 M)
    514 μl of Ammonium sulfate (3.5 M)
    20 μl of Sodium chloride (5 M)
    414 μl of water (100 v/v)
    F7
    • Lithium sulfate 0.3 M -
    • Polyethylene glycol 1,000 18 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    150 μl of Lithium sulfate (2 M)
    360 μl of Polyethylene glycol 1,000 (50 w/v)
    440 μl of water (100 v/v)
    F8
    • Sodium chloride 0.3 M -
    • Polyethylene glycol 1,000 15 w/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    60 μl of Sodium chloride (5 M)
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    590 μl of water (100 v/v)
    F9
    • Potassium chloride 0.25 M -
    • Polyethylene glycol 1,000 12 w/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    62 μl of Potassium chloride (4 M)
    240 μl of Polyethylene glycol 1,000 (50 w/v)
    647 μl of water (100 v/v)
    F10
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol monomethyl ether 350 25 v/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    50 μl of Lithium sulfate (2 M)
    250 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    650 μl of water (100 v/v)
    F11
    • Polyethylene glycol monomethyl ether 350 20 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    750 μl of water (100 v/v)
    F12
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol monomethyl ether 350 15 v/v -
    • HEPES 0.05 M pH 6.5
    pH 6.5
    50 μl of HEPES pH 6.5 (1 M)
    25 μl of Lithium sulfate (2 M)
    150 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    775 μl of water (100 v/v)
    G1
    • 2-methyl-2,4-pentanediol BCC 18 v/v -
    • Spermine 0.005 M -
    • Sodium acetate 0.05 M pH 4.5
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (1 M)
    50 μl of Spermine (0.1 M)
    180 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    720 μl of water (100 v/v)
    G2
    • Sodium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 24 v/v -
    • Sodium acetate 0.05 M pH 4.5
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (1 M)
    240 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    20 μl of Sodium chloride (5 M)
    690 μl of water (100 v/v)
    G3
    • Potassium chloride 0.15 M -
    • 2-methyl-2,4-pentanediol BCC 32 v/v -
    • Sodium acetate 0.05 M pH 4.5
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (1 M)
    320 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    37 μl of Potassium chloride (4 M)
    592 μl of water (100 v/v)
    G4
    • 2-methyl-2,4-pentanediol BCC 21 v/v -
    • Magnesium chloride 0.01 M -
    • Sodium acetate 0.05 M pH 5
    pH 5
    210 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Sodium acetate pH 5 (1 M)
    5 μl of Magnesium chloride (2 M)
    735 μl of water (100 v/v)
    G5
    • Sodium chloride 0.1 M -
    • Potassium chloride 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 14 v/v -
    • Spermine 0.005 M -
    • Sodium acetate 0.05 M pH 5
    pH 5
    50 μl of Spermine (0.1 M)
    140 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Sodium acetate pH 5 (1 M)
    20 μl of Sodium chloride (5 M)
    25 μl of Potassium chloride (4 M)
    715 μl of water (100 v/v)
    G6
    • Polyethylene glycol 400 15 v/v -
    • Lithium chloride 0.05 M -
    • Sodium acetate 0.05 M pH 5
    pH 5
    150 μl of Polyethylene glycol 400 (100 v/v)
    5 μl of Lithium chloride (10 M)
    50 μl of Sodium acetate pH 5 (1 M)
    795 μl of water (100 v/v)
    G7
    • 2-methyl-2,4-pentanediol BCC 21 v/v -
    • MES 0.05 M pH 5.5
    pH 5.5
    210 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of MES pH 5.5 (1 M)
    740 μl of water (100 v/v)
    G8
    • 2-methyl-2,4-pentanediol BCC 21 v/v -
    • Magnesium chloride 0.02 M -
    • MES 0.05 M pH 5.5
    pH 5.5
    210 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Magnesium chloride (2 M)
    50 μl of MES pH 5.5 (1 M)
    730 μl of water (100 v/v)
    G9
    • Polyethylene glycol 2,000 12 w/v -
    • MES 0.05 M pH 5.5
    pH 5.5
    240 μl of Polyethylene glycol 2,000 (50 w/v)
    50 μl of MES pH 5.5 (1 M)
    710 μl of water (100 v/v)
    G10
    • 2-methyl-2,4-pentanediol BCC 21 v/v -
    • Magnesium sulfate 0.02 M -
    • MES 0.05 M pH 5.5
    pH 5.5
    8 μl of Magnesium sulfate (2.5 M)
    210 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of MES pH 5.5 (1 M)
    732 μl of water (100 v/v)
    G11
    • Polyethylene glycol 400 15 v/v -
    • Spermine 0.005 M -
    • MES 0.05 M pH 5.5
    pH 5.5
    150 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Spermine (0.1 M)
    50 μl of MES pH 5.5 (1 M)
    750 μl of water (100 v/v)
    G12
    • Ammonium sulfate 1.5 M -
    • Glycerol 10 v/v -
    • MES 0.05 M pH 5.5
    pH 5.5
    428 μl of Ammonium sulfate (3.5 M)
    100 μl of Glycerol (100 v/v)
    50 μl of MES pH 5.5 (1 M)
    421 μl of water (100 v/v)
    H1
    • Sodium chloride 0.05 M -
    • Potassium chloride 0.1 M -
    • Polyethylene glycol 2,000 25 w/v -
    • Calcium chloride 0.01 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    5 μl of Calcium chloride (2 M)
    10 μl of Sodium chloride (5 M)
    500 μl of Polyethylene glycol 2,000 (50 w/v)
    25 μl of Potassium chloride (4 M)
    410 μl of water (100 v/v)
    H2
    • Sodium chloride 0.25 M -
    • Polyethylene glycol 3,350 20 w/v -
    • Spermine 0.005 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of Spermine (0.1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of BIS-TRIS pH 7 (1 M)
    50 μl of Sodium chloride (5 M)
    450 μl of water (100 v/v)
    H3
    • Potassium chloride 0.1 M -
    • Polyethylene glycol 8,000 12 w/v -
    • Barium chloride 0.005 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    5 μl of Barium chloride (1 M)
    25 μl of Potassium chloride (4 M)
    240 μl of Polyethylene glycol 8,000 (50 w/v)
    680 μl of water (100 v/v)
    H4
    • Sodium chloride 0.3 M -
    • Polyethylene glycol 20,000 8 w/v -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    50 μl of BIS-TRIS pH 7 (1 M)
    60 μl of Sodium chloride (5 M)
    266 μl of Polyethylene glycol 20,000 (30 w/v)
    623 μl of water (100 v/v)
    H5
    • Sodium chloride 0.05 M -
    • Lithium chloride 0.2 M -
    • Polyethylene glycol 2,000 20 w/v -
    • Calcium chloride 0.01 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    20 μl of Lithium chloride (10 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    5 μl of Calcium chloride (2 M)
    10 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    515 μl of water (100 v/v)
    H6
    • Potassium chloride 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • Spermine 0.002 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    20 μl of Spermine (0.1 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    605 μl of water (100 v/v)
    H7
    • Potassium chloride 0.05 M -
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • Magnesium sulfate 0.02 M -
    • BIS-TRIS 0.05 M pH 7
    pH 7
    8 μl of Magnesium sulfate (2.5 M)
    50 μl of BIS-TRIS pH 7 (1 M)
    50 μl of Lithium sulfate (2 M)
    12 μl of Potassium chloride (4 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    579 μl of water (100 v/v)
    H8
    • Sodium chloride 0.1 M -
    • Polyethylene glycol 20,000 6 w/v -
    • Barium chloride 0.002 M -
    • BIS-TRIS 0.05 M pH 7.5
    pH 7.5
    2 μl of Barium chloride (1 M)
    20 μl of Sodium chloride (5 M)
    200 μl of Polyethylene glycol 20,000 (30 w/v)
    50 μl of BIS-TRIS pH 7.5 (1 M)
    728 μl of water (100 v/v)
    H9
    • Potassium chloride 0.1 M -
    • Polyethylene glycol 8,000 7 w/v -
    • Spermine 0.002 M -
    • BIS-TRIS 0.05 M pH 7.5
    pH 7.5
    20 μl of Spermine (0.1 M)
    50 μl of BIS-TRIS pH 7.5 (1 M)
    25 μl of Potassium chloride (4 M)
    140 μl of Polyethylene glycol 8,000 (50 w/v)
    765 μl of water (100 v/v)
    H10
    • Lithium sulfate 0.05 M -
    • Polyethylene glycol 3,350 15 w/v -
    • Magnesium sulfate 0.03 M -
    • BIS-TRIS 0.05 M pH 8
    pH 8
    12 μl of Magnesium sulfate (2.5 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Lithium sulfate (2 M)
    50 μl of BIS-TRIS pH 8 (1 M)
    613 μl of water (100 v/v)
    H11
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 3,350 9 w/v -
    • BIS-TRIS 0.05 M pH 8.5
    pH 8.5
    50 μl of BIS-TRIS pH 8.5 (1 M)
    180 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    730 μl of water (100 v/v)
    H12
    • Polyethylene glycol 3,350 18 w/v -
    • Magnesium chloride 0.052 M -
    • BIS-TRIS 0.05 M pH 8.5
    pH 8.5
    50 μl of BIS-TRIS pH 8.5 (1 M)
    360 μl of Polyethylene glycol 3,350 (50 w/v)
    26 μl of Magnesium chloride (2 M)
    564 μl of water (100 v/v)