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Supplemental Information

Screen Description

Helix

Created 2014-10-14.

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Helix

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Screen Structure

Helix

Well Count 96
Number of distinct ingredients 33
Internal diversity (score mean dist) 0.874

Screen Composition

2025-08-16T01:43:05.644987 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Helix
Buffer 132
Cryoprotectant 6
Organic (non-volatile) 45
Polymer 52
Salt 145

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Helix Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Potassium chloride 0.05 M -
  • Lithium chloride 0.1 M -
  • Polyethylene glycol 400 25 v/v -
  • Spermine 0.012 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
12.5 μl of Potassium chloride (4 M)
120.0 μl of Spermine (0.1 M)
507.5 μl of water (100 v/v)
A2
  • Polyethylene glycol 400 25 v/v -
  • Spermine 0.001 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
10.0 μl of Spermine (0.1 M)
640.0 μl of water (100 v/v)
A3
  • Sodium chloride 0.1 M -
  • Lithium chloride 0.05 M -
  • Polyethylene glycol 400 25 v/v -
  • Magnesium chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
5.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
5.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
A4
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 400 25 v/v -
  • Cobalt (III) Hexamine chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
50.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
100.0 μl of MES pH 6.5 (0.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium chloride (4 M)
550.0 μl of water (100 v/v)
A5
  • Lithium chloride 0.1 M -
  • Polyethylene glycol 400 25 v/v -
  • Manganese (II) chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Manganese (II) chloride (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
630.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 400 25 v/v -
  • Strontium chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
50.0 μl of Strontium chloride (0.1 M)
580.0 μl of water (100 v/v)
A7
  • Potassium chloride 0.05 M -
  • Lithium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Spermine 0.012 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
12.5 μl of Potassium chloride (4 M)
120.0 μl of Spermine (0.1 M)
407.5 μl of water (100 v/v)
A8
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Spermine 0.001 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Spermine (0.1 M)
540.0 μl of water (100 v/v)
A9
  • Sodium chloride 0.1 M -
  • Lithium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Magnesium chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
5.0 μl of Magnesium chloride (2 M)
20.0 μl of Sodium chloride (5 M)
515.0 μl of water (100 v/v)
A10
  • Potassium chloride 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Cobalt (III) Hexamine chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
100.0 μl of MES pH 6.5 (0.5 M)
50.0 μl of Potassium chloride (4 M)
450.0 μl of water (100 v/v)
A11
  • Lithium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Manganese (II) chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Manganese (II) chloride (1 M)
530.0 μl of water (100 v/v)
A12
  • Sodium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Strontium chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (0.5 M)
20.0 μl of Sodium chloride (5 M)
50.0 μl of Strontium chloride (0.1 M)
480.0 μl of water (100 v/v)
B1
  • Potassium chloride 0.05 M -
  • Lithium chloride 0.1 M -
  • Polyethylene glycol 4,000 17 w/v -
  • Spermine 0.012 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
12.5 μl of Potassium chloride (4 M)
120.0 μl of Spermine (0.1 M)
417.5 μl of water (100 v/v)
B2
  • Polyethylene glycol 4,000 17 w/v -
  • Spermine 0.001 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.0 μl of Spermine (0.1 M)
550.0 μl of water (100 v/v)
B3
  • Sodium chloride 0.1 M -
  • Lithium chloride 0.05 M -
  • Polyethylene glycol 4,000 17 w/v -
  • Magnesium chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
5.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
5.0 μl of Magnesium chloride (2 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
B4
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 4,000 17 w/v -
  • Cobalt (III) Hexamine chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
50.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
100.0 μl of MES pH 6.5 (0.5 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Potassium chloride (4 M)
460.0 μl of water (100 v/v)
B5
  • Lithium chloride 0.1 M -
  • Polyethylene glycol 4,000 17 w/v -
  • Manganese (II) chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Manganese (II) chloride (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
540.0 μl of water (100 v/v)
B6
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 4,000 17 w/v -
  • Strontium chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
50.0 μl of Strontium chloride (0.1 M)
490.0 μl of water (100 v/v)
B7
  • Potassium chloride 0.05 M -
  • Lithium chloride 0.1 M -
  • di-Sodium malonate 2.6 M -
  • Spermine 0.012 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
12.5 μl of Potassium chloride (4 M)
120.0 μl of Spermine (0.1 M)
650.0 μl of di-Sodium malonate (4 M)
107.5 μl of water (100 v/v)
B8
  • di-Sodium malonate 2.6 M -
  • Spermine 0.001 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Spermine (0.1 M)
650.0 μl of di-Sodium malonate (4 M)
240.0 μl of water (100 v/v)
B9
  • Sodium chloride 0.1 M -
  • Lithium chloride 0.05 M -
  • di-Sodium malonate 2.6 M -
  • Magnesium chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
5.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
5.0 μl of Magnesium chloride (2 M)
20.0 μl of Sodium chloride (5 M)
650.0 μl of di-Sodium malonate (4 M)
220.0 μl of water (100 v/v)
B10
  • Potassium chloride 0.2 M -
  • di-Sodium malonate 2.6 M -
  • Cobalt (III) Hexamine chloride 0.005 M -
  • MES 0.05 M pH 6.5
pH 6.5
50.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
100.0 μl of MES pH 6.5 (0.5 M)
50.0 μl of Potassium chloride (4 M)
650.0 μl of di-Sodium malonate (4 M)
150.0 μl of water (100 v/v)
B11
  • Lithium chloride 0.1 M -
  • di-Sodium malonate 2.6 M -
  • Manganese (II) chloride 0.01 M -
  • MES 0.05 M pH 6.5
pH 6.5
10.0 μl of Lithium chloride (10 M)
100.0 μl of MES pH 6.5 (0.5 M)
10.0 μl of Manganese (II) chloride (1 M)
650.0 μl of di-Sodium malonate (4 M)
230.0 μl of water (100 v/v)
B12
  • Sodium chloride 0.1 M -
  • di-Sodium malonate 2.6 M -
  • Strontium chloride 0.002 M -
  • MES 0.05 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
20.0 μl of Sodium chloride (5 M)
20.0 μl of Strontium chloride (0.1 M)
650.0 μl of di-Sodium malonate (4 M)
210.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 0.1 M -
  • Polyethylene glycol 200 44 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
28.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
440.0 μl of Polyethylene glycol 200 (100 v/v)
481.4 μl of water (100 v/v)
C2
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 400 40 v/v -
  • Calcium chloride 0.01 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
5.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium chloride (4 M)
495.0 μl of water (100 v/v)
C3
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 1,000 37 w/v -
  • Spermine 0.002 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
740.0 μl of Polyethylene glycol 1,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
20.0 μl of Spermine (0.1 M)
170.0 μl of water (100 v/v)
C4
  • Sodium chloride 0.1 M -
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 2,000 35 w/v -
  • Magnesium chloride 0.02 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
10.0 μl of Magnesium chloride (2 M)
700.0 μl of Polyethylene glycol 2,000 (50 w/v)
50.0 μl of Potassium chloride (4 M)
20.0 μl of Sodium chloride (5 M)
170.0 μl of water (100 v/v)
C5
  • Potassium chloride 0.05 M -
  • Polyethylene glycol 3,350 33 w/v -
  • Spermine 0.005 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
660.0 μl of Polyethylene glycol 3,350 (50 w/v)
12.5 μl of Potassium chloride (4 M)
50.0 μl of Spermine (0.1 M)
227.5 μl of water (100 v/v)
C6
  • Ammonium sulfate 0.1 M -
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium formate 1 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
28.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
142.9 μl of Sodium formate (7 M)
358.6 μl of water (100 v/v)
C7
  • Polyethylene glycol 20,000 20 w/v -
  • Sodium formate 1 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
666.7 μl of Polyethylene glycol 20,000 (30 w/v)
142.9 μl of Sodium formate (7 M)
140.5 μl of water (100 v/v)
C8
  • Lithium sulfate 0.05 M -
  • Polyethylene glycol monomethyl ether 500 40 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
25.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
525.0 μl of water (100 v/v)
C9
  • tri-Sodium citrate 0.1 M -
  • Polyethylene glycol monomethyl ether 500 40 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
62.5 μl of tri-Sodium citrate (1.6 M)
487.5 μl of water (100 v/v)
C10
  • Ammonium sulfate 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Spermine 0.002 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
28.6 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
20.0 μl of Spermine (0.1 M)
501.4 μl of water (100 v/v)
C11
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of BIS-TRIS pH 7 (1 M)
600.0 μl of water (100 v/v)
C12
  • Sodium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Magnesium chloride 0.02 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of BIS-TRIS pH 7 (1 M)
10.0 μl of Magnesium chloride (2 M)
20.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
D1
  • Potassium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 27 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
270.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of BIS-TRIS pH 7 (1 M)
25.0 μl of Potassium chloride (4 M)
655.0 μl of water (100 v/v)
D2
  • 2-methyl-2,4-pentanediol BCC 24 v/v -
  • Polyethylene glycol 400 5 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
240.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of BIS-TRIS pH 7 (1 M)
50.0 μl of Polyethylene glycol 400 (100 v/v)
660.0 μl of water (100 v/v)
D3
  • Lithium sulfate 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of BIS-TRIS pH 7 (1 M)
100.0 μl of Lithium sulfate (2 M)
650.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 5 w/v -
  • Ammonium sulfate 1 M -
  • Glycerol 20 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
285.7 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of Polyethylene glycol 3,350 (50 w/v)
364.3 μl of water (100 v/v)
D5
  • Polyethylene glycol monomethyl ether 500 5 v/v -
  • Ammonium sulfate 1.2 M -
  • Glycerol 10 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
342.9 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
100.0 μl of Glycerol (100 v/v)
50.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
457.1 μl of water (100 v/v)
D6
  • Ammonium sulfate 1.4 M -
  • Glycerol 25 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
400.0 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
250.0 μl of Glycerol (100 v/v)
300.0 μl of water (100 v/v)
D7
  • Potassium chloride 0.15 M -
  • Ammonium sulfate 1.6 M -
  • Glycerol 25 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
457.1 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
250.0 μl of Glycerol (100 v/v)
37.5 μl of Potassium chloride (4 M)
205.4 μl of water (100 v/v)
D8
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 1.8 M -
  • Glycerol 25 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
514.3 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
250.0 μl of Glycerol (100 v/v)
20.0 μl of Sodium chloride (5 M)
165.7 μl of water (100 v/v)
D9
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 5 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
571.4 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
50.0 μl of Polyethylene glycol 400 (100 v/v)
328.6 μl of water (100 v/v)
D10
  • Potassium chloride 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
485.0 μl of water (100 v/v)
D11
  • Lithium chloride 0.2 M -
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
20.0 μl of Lithium chloride (10 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
490.0 μl of water (100 v/v)
D12
  • Sodium chloride 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
490.0 μl of water (100 v/v)
E1
  • Potassium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Potassium chloride (4 M)
725.0 μl of water (100 v/v)
E2
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • Spermine 0.005 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
50.0 μl of Spermine (0.1 M)
800.0 μl of water (100 v/v)
E3
  • Sodium chloride 0.1 M -
  • Potassium chloride 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
50.0 μl of Potassium chloride (4 M)
20.0 μl of Sodium chloride (5 M)
780.0 μl of water (100 v/v)
E4
  • Sodium chloride 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
660.0 μl of water (100 v/v)
E5
  • Lithium sulfate 0.05 M -
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Lithium sulfate (2 M)
775.0 μl of water (100 v/v)
E6
  • Sodium chloride 0.3 M -
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of HEPES pH 6.5 (1 M)
60.0 μl of Sodium chloride (5 M)
590.0 μl of water (100 v/v)
E7
  • Sodium chloride 0.05 M -
  • Polyethylene glycol 400 25 v/v -
  • Magnesium chloride 0.01 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
5.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
10.0 μl of Sodium chloride (5 M)
685.0 μl of water (100 v/v)
E8
  • Potassium chloride 0.2 M -
  • Polyethylene glycol 400 10 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Potassium chloride (4 M)
800.0 μl of water (100 v/v)
E9
  • Lithium sulfate 0.05 M -
  • Polyethylene glycol 1,000 18 w/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Lithium sulfate (2 M)
360.0 μl of Polyethylene glycol 1,000 (50 w/v)
565.0 μl of water (100 v/v)
E10
  • Lithium sulfate 1.5 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
750.0 μl of Lithium sulfate (2 M)
200.0 μl of water (100 v/v)
E11
  • Sodium chloride 0.1 M -
  • Potassium chloride 0.1 M -
  • Lithium sulfate 1.9 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
633.3 μl of Lithium sulfate (3 M)
25.0 μl of Potassium chloride (4 M)
20.0 μl of Sodium chloride (5 M)
271.7 μl of water (100 v/v)
E12
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • Magnesium chloride 0.02 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
10.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
640.0 μl of water (100 v/v)
F1
  • Lithium sulfate 0.05 M -
  • Polyethylene glycol monomethyl ether 2,000 10 w/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
725.0 μl of water (100 v/v)
F2
  • Polyethylene glycol monomethyl ether 2,000 14 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
280.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
670.0 μl of water (100 v/v)
F3
  • Lithium chloride 0.1 M -
  • Polyethylene glycol monomethyl ether 500 10 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
10.0 μl of Lithium chloride (10 M)
100.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
840.0 μl of water (100 v/v)
F4
  • Potassium chloride 0.05 M -
  • Ammonium sulfate 1.1 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
314.3 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 7 (1 M)
12.5 μl of Potassium chloride (4 M)
623.2 μl of water (100 v/v)
F5
  • Lithium sulfate 0.05 M -
  • Ammonium sulfate 1.4 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
400.0 μl of Ammonium sulfate (3.5 M)
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Lithium sulfate (2 M)
525.0 μl of water (100 v/v)
F6
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 1.8 M -
  • Lithium chloride 0.01 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
514.3 μl of Ammonium sulfate (3.5 M)
50.0 μl of HEPES pH 6.5 (1 M)
1.0 μl of Lithium chloride (10 M)
20.0 μl of Sodium chloride (5 M)
414.7 μl of water (100 v/v)
F7
  • Lithium sulfate 0.3 M -
  • Polyethylene glycol 1,000 18 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
150.0 μl of Lithium sulfate (2 M)
360.0 μl of Polyethylene glycol 1,000 (50 w/v)
440.0 μl of water (100 v/v)
F8
  • Sodium chloride 0.3 M -
  • Polyethylene glycol 1,000 15 w/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
60.0 μl of Sodium chloride (5 M)
590.0 μl of water (100 v/v)
F9
  • Potassium chloride 0.25 M -
  • Polyethylene glycol 1,000 12 w/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 1,000 (50 w/v)
62.5 μl of Potassium chloride (4 M)
647.5 μl of water (100 v/v)
F10
  • Lithium sulfate 0.1 M -
  • Polyethylene glycol monomethyl ether 350 25 v/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
250.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
650.0 μl of water (100 v/v)
F11
  • Polyethylene glycol monomethyl ether 350 20 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
750.0 μl of water (100 v/v)
F12
  • Lithium sulfate 0.05 M -
  • Polyethylene glycol monomethyl ether 350 15 v/v -
  • HEPES 0.05 M pH 6.5
pH 6.5
50.0 μl of HEPES pH 6.5 (1 M)
25.0 μl of Lithium sulfate (2 M)
150.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
775.0 μl of water (100 v/v)
G1
  • 2-methyl-2,4-pentanediol BCC 18 v/v -
  • Spermine 0.005 M -
  • Sodium acetate 0.05 M pH 4.5
pH 4.5
180.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (1 M)
50.0 μl of Spermine (0.1 M)
720.0 μl of water (100 v/v)
G2
  • Sodium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 24 v/v -
  • Sodium acetate 0.05 M pH 4.5
pH 4.5
240.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (1 M)
20.0 μl of Sodium chloride (5 M)
690.0 μl of water (100 v/v)
G3
  • Potassium chloride 0.15 M -
  • 2-methyl-2,4-pentanediol BCC 32 v/v -
  • Sodium acetate 0.05 M pH 4.5
pH 4.5
320.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
37.5 μl of Potassium chloride (4 M)
50.0 μl of Sodium acetate pH 4.5 (1 M)
592.5 μl of water (100 v/v)
G4
  • 2-methyl-2,4-pentanediol BCC 21 v/v -
  • Magnesium chloride 0.01 M -
  • Sodium acetate 0.05 M pH 5
pH 5
210.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
5.0 μl of Magnesium chloride (2 M)
50.0 μl of Sodium acetate pH 5 (1 M)
735.0 μl of water (100 v/v)
G5
  • Sodium chloride 0.1 M -
  • Potassium chloride 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 14 v/v -
  • Spermine 0.005 M -
  • Sodium acetate 0.05 M pH 5
pH 5
140.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
25.0 μl of Potassium chloride (4 M)
50.0 μl of Sodium acetate pH 5 (1 M)
20.0 μl of Sodium chloride (5 M)
50.0 μl of Spermine (0.1 M)
715.0 μl of water (100 v/v)
G6
  • Polyethylene glycol 400 15 v/v -
  • Lithium chloride 0.05 M -
  • Sodium acetate 0.05 M pH 5
pH 5
5.0 μl of Lithium chloride (10 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium acetate pH 5 (1 M)
795.0 μl of water (100 v/v)
G7
  • 2-methyl-2,4-pentanediol BCC 21 v/v -
  • MES 0.05 M pH 5.5
pH 5.5
210.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of MES pH 5.5 (1 M)
740.0 μl of water (100 v/v)
G8
  • 2-methyl-2,4-pentanediol BCC 21 v/v -
  • Magnesium chloride 0.02 M -
  • MES 0.05 M pH 5.5
pH 5.5
210.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of MES pH 5.5 (1 M)
10.0 μl of Magnesium chloride (2 M)
730.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 2,000 12 w/v -
  • MES 0.05 M pH 5.5
pH 5.5
50.0 μl of MES pH 5.5 (1 M)
240.0 μl of Polyethylene glycol 2,000 (50 w/v)
710.0 μl of water (100 v/v)
G10
  • 2-methyl-2,4-pentanediol BCC 21 v/v -
  • Magnesium sulfate 0.02 M -
  • MES 0.05 M pH 5.5
pH 5.5
210.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
50.0 μl of MES pH 5.5 (1 M)
8.0 μl of Magnesium sulfate (2.5 M)
732.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 400 15 v/v -
  • Spermine 0.005 M -
  • MES 0.05 M pH 5.5
pH 5.5
50.0 μl of MES pH 5.5 (1 M)
150.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Spermine (0.1 M)
750.0 μl of water (100 v/v)
G12
  • Ammonium sulfate 1.5 M -
  • Glycerol 10 v/v -
  • MES 0.05 M pH 5.5
pH 5.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Glycerol (100 v/v)
50.0 μl of MES pH 5.5 (1 M)
421.4 μl of water (100 v/v)
H1
  • Sodium chloride 0.05 M -
  • Potassium chloride 0.1 M -
  • Polyethylene glycol 2,000 25 w/v -
  • Calcium chloride 0.01 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
5.0 μl of Calcium chloride (2 M)
500.0 μl of Polyethylene glycol 2,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
10.0 μl of Sodium chloride (5 M)
410.0 μl of water (100 v/v)
H2
  • Sodium chloride 0.25 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Spermine 0.005 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium chloride (5 M)
50.0 μl of Spermine (0.1 M)
450.0 μl of water (100 v/v)
H3
  • Potassium chloride 0.1 M -
  • Polyethylene glycol 8,000 12 w/v -
  • Barium chloride 0.005 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
5.0 μl of Barium chloride (1 M)
240.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
680.0 μl of water (100 v/v)
H4
  • Sodium chloride 0.3 M -
  • Polyethylene glycol 20,000 8 w/v -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
266.7 μl of Polyethylene glycol 20,000 (30 w/v)
60.0 μl of Sodium chloride (5 M)
623.3 μl of water (100 v/v)
H5
  • Sodium chloride 0.05 M -
  • Lithium chloride 0.2 M -
  • Polyethylene glycol 2,000 20 w/v -
  • Calcium chloride 0.01 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
5.0 μl of Calcium chloride (2 M)
20.0 μl of Lithium chloride (10 M)
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
10.0 μl of Sodium chloride (5 M)
515.0 μl of water (100 v/v)
H6
  • Potassium chloride 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • Spermine 0.002 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
20.0 μl of Spermine (0.1 M)
605.0 μl of water (100 v/v)
H7
  • Potassium chloride 0.05 M -
  • Lithium sulfate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • Magnesium sulfate 0.02 M -
  • BIS-TRIS 0.05 M pH 7
pH 7
50.0 μl of BIS-TRIS pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
8.0 μl of Magnesium sulfate (2.5 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium chloride (4 M)
579.5 μl of water (100 v/v)
H8
  • Sodium chloride 0.1 M -
  • Polyethylene glycol 20,000 6 w/v -
  • Barium chloride 0.002 M -
  • BIS-TRIS 0.05 M pH 7.5
pH 7.5
50.0 μl of BIS-TRIS pH 7.5 (1 M)
2.0 μl of Barium chloride (1 M)
200.0 μl of Polyethylene glycol 20,000 (30 w/v)
20.0 μl of Sodium chloride (5 M)
728.0 μl of water (100 v/v)
H9
  • Potassium chloride 0.1 M -
  • Polyethylene glycol 8,000 7 w/v -
  • Spermine 0.002 M -
  • BIS-TRIS 0.05 M pH 7.5
pH 7.5
50.0 μl of BIS-TRIS pH 7.5 (1 M)
140.0 μl of Polyethylene glycol 8,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
20.0 μl of Spermine (0.1 M)
765.0 μl of water (100 v/v)
H10
  • Lithium sulfate 0.05 M -
  • Polyethylene glycol 3,350 15 w/v -
  • Magnesium sulfate 0.03 M -
  • BIS-TRIS 0.05 M pH 8
pH 8
50.0 μl of BIS-TRIS pH 8 (1 M)
25.0 μl of Lithium sulfate (2 M)
12.0 μl of Magnesium sulfate (2.5 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
613.0 μl of water (100 v/v)
H11
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 3,350 9 w/v -
  • BIS-TRIS 0.05 M pH 8.5
pH 8.5
50.0 μl of BIS-TRIS pH 8.5 (1 M)
180.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
730.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 3,350 18 w/v -
  • Magnesium chloride 0.052 M -
  • BIS-TRIS 0.05 M pH 8.5
pH 8.5
50.0 μl of BIS-TRIS pH 8.5 (1 M)
26.0 μl of Magnesium chloride (2 M)
360.0 μl of Polyethylene glycol 3,350 (50 w/v)
564.0 μl of water (100 v/v)