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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
TOP96
Barcodes
TOP96
No barcodes found.
Screen Structure
TOP96
Well Count | 96 |
---|---|
Number of distinct ingredients | 52 |
Internal diversity (score mean dist) | 0.911 |
Screen Composition
ingredient type | TOP96 |
---|---|
Buffer | 88 |
Complex ingredient | 1 |
Organic (non-volatile) | 4 |
Organic (volatile) | 1 |
Polymer | 74 |
Salt | 99 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 220.0 μl of water (100 v/v) |
A2 |
| NA | 666.7 μl of Ammonium sulfate (3 M) 333.3 μl of water (100 v/v) |
A3 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate (2 M) 500.0 μl of water (100 v/v) |
A4 |
| pH 8.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
A5 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v) |
A6 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
A7 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Polyethylene glycol 400 (50 w/v) 193.3 μl of water (100 v/v) |
A8 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 350.0 μl of tri-Sodium citrate (4 M) 550.0 μl of water (100 v/v) |
A9 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
A10 |
| pH 8.5 | 40.0 μl of Lithium sulfate (5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 260.0 μl of water (100 v/v) |
A11 |
| NA | 571.4 μl of Sodium formate pH 7 (7 M) 428.6 μl of water (100 v/v) |
A12 |
| pH 6.5 | 100.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v) |
B1 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 20,000 (30 w/v) 500.0 μl of water (100 v/v) |
B3 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
B4 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 233.3 μl of water (100 v/v) |
B5 |
| NA | 100.0 μl of Calcium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v) |
B7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium formate pH 7 (7 M) 571.4 μl of water (100 v/v) |
B8 |
| pH 5.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |
B9 |
| NA | 400.0 μl of tri-Sodium citrate (4 M) 600.0 μl of water (100 v/v) |
B10 |
| pH 7.5 | 100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 560.0 μl of Polyethylene glycol 400 (50 w/v) 240.0 μl of water (100 v/v) |
B11 |
| NA | 200.0 μl of Ammonium chloride pH 6.3 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
B12 |
| NA | 200.0 μl of Magnesium formate pH 5.9 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
C1 |
| pH 4.6 | 66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 333.3 μl of water (100 v/v) |
C2 |
| NA | 705.9 μl of di-Sodium malonate pH 7 (3.4 M) 294.1 μl of water (100 v/v) |
C3 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 40.0 μl of Lithium sulfate (5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 360.0 μl of water (100 v/v) |
C4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of Potassium Sodium tartrate (1.5 M) 466.7 μl of water (100 v/v) |
C5 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 233.3 μl of water (100 v/v) |
C6 |
| pH 4.6 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v) |
C7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
C8 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v) |
C9 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
C10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 320.0 μl of water (100 v/v) |
C11 |
| pH 6.5 | 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 200.0 μl of water (100 v/v) |
C12 |
| pH 5.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
D1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Lithium sulfate (5 M) 600.0 μl of water (100 v/v) |
D2 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 25.0 μl of tri-Sodium citrate (4 M) 475.0 μl of water (100 v/v) |
D3 |
| NA | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
D4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Potassium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 440.0 μl of water (100 v/v) |
D6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Lithium sulfate (5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 360.0 μl of water (100 v/v) |
D7 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 333.3 μl of water (100 v/v) |
D8 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v) |
D9 |
| pH 4.6 | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 233.3 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 40.0 μl of Lithium sulfate (5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 360.0 μl of water (100 v/v) |
D11 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
D12 |
| pH 6.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 233.3 μl of water (100 v/v) |
E1 |
| pH 5.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 233.3 μl of water (100 v/v) |
E2 |
| NA | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 80.0 μl of Magnesium chloride (2.5 M) 600.0 μl of Polyethylene glycol 400 (50 w/v) 220.0 μl of water (100 v/v) |
E4 |
| pH 7.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 233.3 μl of water (100 v/v) |
E5 |
| NA | 500.0 μl of Sodium formate pH 7 (7 M) 500.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 800.0 μl of Magnesium sulfate (2 M) 100.0 μl of water (100 v/v) |
E7 |
| NA | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 520.0 μl of water (100 v/v) |
E8 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 333.3 μl of water (100 v/v) |
E9 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v) |
E10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 600.0 μl of water (100 v/v) |
E11 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 285.7 μl of Sodium formate pH 7 (7 M) 614.3 μl of water (100 v/v) |
E12 |
| pH 7.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |
F1 |
| pH 5.6 | 666.7 μl of Ammonium sulfate (3 M) 133.3 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 100.0 μl of water (100 v/v) |
F2 |
| pH 7.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
F3 |
| NA | 66.7 μl of Ammonium sulfate (3 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 533.3 μl of water (100 v/v) |
F4 |
| pH 6.5 | 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 250.0 μl of tri-Sodium citrate (4 M) 650.0 μl of water (100 v/v) |
F5 |
| pH 6.5 | 66.7 μl of Ammonium sulfate (3 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 333.3 μl of water (100 v/v) |
F6 |
| NA | 20.0 μl of Ammonium nitrate pH 6.3 (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
F7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate pH 6.9 (8 M) 575.0 μl of water (100 v/v) |
F8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate pH 6.9 (3 M) 533.3 μl of water (100 v/v) |
F9 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
F10 |
| NA | 100.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F11 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F12 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 w/v) 100.0 μl of Calcium chloride (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 500.0 μl of water (100 v/v) |
G1 |
| pH 6 | 200.0 μl of Calcium acetate (1 M) 33.3 μl of MES pH 6 (3 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 366.7 μl of water (100 v/v) |
G2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Sodium sulfate (1 M) 400.0 μl of water (100 v/v) |
G3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 5.0 μl of Zinc sulfate (2 M) 645.0 μl of water (100 v/v) |
G4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of di-Sodium tartrate (1.5 M) 466.7 μl of water (100 v/v) |
G5 |
| NA | 600.0 μl of Tacsimate pH 7 (100 v/v) 400.0 μl of water (100 v/v) |
G6 |
| pH 5.6 | 166.7 μl of Ammonium sulfate (3 M) 200.0 μl of Lithium sulfate (5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 533.3 μl of water (100 v/v) |
G7 |
| NA | 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
G8 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v) |
G9 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Ammonium tartrate (2 M) 500.0 μl of water (100 v/v) |
G10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 350.0 μl of water (100 v/v) |
G11 |
| pH 6.5 | 666.7 μl of Ammonium sulfate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 40.0 μl of Sodium chloride (5 M) 193.3 μl of water (100 v/v) |
G12 |
| pH 7.5 | 533.3 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Sodium chloride (5 M) 346.7 μl of water (100 v/v) |
H1 |
| pH 7.5 | 20.0 μl of Ammonium formate pH 6.6 (10 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 480.0 μl of water (100 v/v) |
H2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of tri-Lithium citrate (1.5 M) 466.7 μl of water (100 v/v) |
H3 |
| NA | 100.0 μl of Ammonium iodide (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
H4 |
| pH 6.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
H5 |
| NA | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
H6 |
| pH 8.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
H7 |
| NA | 20.0 μl of Ammonium fluoride (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
H8 |
| pH 5.5 | 100.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 460.0 μl of water (100 v/v) |
H9 |
| pH 8 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H10 |
| pH 8.5 | 66.7 μl of Ammonium sulfate (3 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 333.3 μl of water (100 v/v) |
H11 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 860.0 μl of Sodium chloride (5 M) 40.0 μl of water (100 v/v) |