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Supplemental Information

Screen Description

TOP96

Created 2014-10-14.

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Barcodes

TOP96

No barcodes found.

Screen Structure

TOP96

Well Count 96
Number of distinct ingredients 52
Internal diversity (score mean dist) 0.911

Screen Composition

2025-08-16T06:23:59.265741 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type TOP96
Buffer 88
Complex ingredient 1
Organic (non-volatile) 4
Organic (volatile) 1
Polymer 74
Salt 99

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
TOP96 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
A2
  • Ammonium sulfate 2 M -
NA
666.7 μl of Ammonium sulfate (3 M)
333.3 μl of water (100 v/v)
A3
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate (2 M)
500.0 μl of water (100 v/v)
A4
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,350 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
A7
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
  • Polyethylene glycol 400 2 w/v -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Polyethylene glycol 400 (50 w/v)
193.3 μl of water (100 v/v)
A8
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
350.0 μl of tri-Sodium citrate (4 M)
550.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A10
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
40.0 μl of Lithium sulfate (5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
260.0 μl of water (100 v/v)
A11
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate pH 7 (7 M)
428.6 μl of water (100 v/v)
A12
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B1
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
B3
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
B4
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
233.3 μl of water (100 v/v)
B5
  • Calcium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
B7
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate pH 7 (7 M)
571.4 μl of water (100 v/v)
B8
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
B9
  • tri-Sodium citrate 1.6 M -
NA
400.0 μl of tri-Sodium citrate (4 M)
600.0 μl of water (100 v/v)
B10
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 w/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 400 (50 w/v)
240.0 μl of water (100 v/v)
B11
  • Ammonium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Ammonium chloride pH 6.3 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B12
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate pH 5.9 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
333.3 μl of water (100 v/v)
C2
  • di-Sodium malonate 2.4 M -
NA
705.9 μl of di-Sodium malonate pH 7 (3.4 M)
294.1 μl of water (100 v/v)
C3
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
40.0 μl of Lithium sulfate (5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
360.0 μl of water (100 v/v)
C4
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
C5
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
233.3 μl of water (100 v/v)
C6
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 2 M -
pH 4.6
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
C7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C8
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 1,500 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
320.0 μl of water (100 v/v)
C9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
C10
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
320.0 μl of water (100 v/v)
C11
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of water (100 v/v)
C12
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
D1
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Lithium sulfate (5 M)
600.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
25.0 μl of tri-Sodium citrate (4 M)
475.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 1,500 25 w/v -
NA
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
D4
  • Potassium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
D5
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
440.0 μl of water (100 v/v)
D6
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Lithium sulfate (5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
360.0 μl of water (100 v/v)
D7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
D8
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
D9
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 4.6
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
233.3 μl of water (100 v/v)
D10
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
40.0 μl of Lithium sulfate (5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
360.0 μl of water (100 v/v)
D11
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
D12
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
pH 6.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
233.3 μl of water (100 v/v)
E1
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
233.3 μl of water (100 v/v)
E2
  • Polyethylene glycol 3,350 25 w/v -
NA
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E3
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of Magnesium chloride (2.5 M)
600.0 μl of Polyethylene glycol 400 (50 w/v)
220.0 μl of water (100 v/v)
E4
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
233.3 μl of water (100 v/v)
E5
  • Sodium formate 3.5 M -
NA
500.0 μl of Sodium formate pH 7 (7 M)
500.0 μl of water (100 v/v)
E6
  • MES 0.1 M pH 6.5
  • Magnesium sulfate 1.6 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
800.0 μl of Magnesium sulfate (2 M)
100.0 μl of water (100 v/v)
E7
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
E8
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
333.3 μl of water (100 v/v)
E9
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
E10
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
E11
  • Sodium acetate 0.1 M pH 4.6
  • Sodium formate 2 M -
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate pH 7 (7 M)
614.3 μl of water (100 v/v)
E12
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
F1
  • Potassium Sodium tartrate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium sulfate 2 M -
pH 5.6
666.7 μl of Ammonium sulfate (3 M)
133.3 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
100.0 μl of water (100 v/v)
F2
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
66.7 μl of Ammonium sulfate (3 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
533.3 μl of water (100 v/v)
F4
  • Sodium cacodylate 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
250.0 μl of tri-Sodium citrate (4 M)
650.0 μl of water (100 v/v)
F5
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
F6
  • Ammonium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium nitrate pH 6.3 (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
F7
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate pH 6.9 (8 M)
575.0 μl of water (100 v/v)
F8
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate pH 6.9 (3 M)
533.3 μl of water (100 v/v)
F9
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
F10
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F11
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F12
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 w/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
100.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
G1
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 8,000 20 w/v -
pH 6
200.0 μl of Calcium acetate (1 M)
33.3 μl of MES pH 6 (3 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
366.7 μl of water (100 v/v)
G2
  • Sodium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
400.0 μl of water (100 v/v)
G3
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
5.0 μl of Zinc sulfate (2 M)
645.0 μl of water (100 v/v)
G4
  • di-Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of di-Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
G5
  • Tacsimate 60 v/v -
NA
600.0 μl of Tacsimate pH 7 (100 v/v)
400.0 μl of water (100 v/v)
G6
  • Ammonium sulfate 0.5 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Lithium sulfate 1 M -
pH 5.6
166.7 μl of Ammonium sulfate (3 M)
200.0 μl of Lithium sulfate (5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
533.3 μl of water (100 v/v)
G7
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
G8
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 20 w/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
G9
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
G10
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
G11
  • Sodium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
pH 6.5
666.7 μl of Ammonium sulfate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
193.3 μl of water (100 v/v)
G12
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.6 M -
pH 7.5
533.3 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Sodium chloride (5 M)
346.7 μl of water (100 v/v)
H1
  • Ammonium formate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 20 w/v -
pH 7.5
20.0 μl of Ammonium formate pH 6.6 (10 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
480.0 μl of water (100 v/v)
H2
  • tri-Lithium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Lithium citrate (1.5 M)
466.7 μl of water (100 v/v)
H3
  • Ammonium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Ammonium iodide (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
H4
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
H6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H7
  • Ammonium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H8
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
H9
  • Calcium acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 8,000 10 w/v -
pH 8
200.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H10
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
H11
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H12
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 4.3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)