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Supplemental Information

Screen Description

TOP96

Created 2014-10-14.

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TOP96

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TOP96

    Screen Structure

    TOP96

    Well Count 96
    Number of distinct ingredients 52
    Internal diversity (score mean dist) 0.911

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:44:16.229470 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type TOP96
    Buffer 88
    Complex ingredient 1
    Organic (non-volatile) 4
    Organic (volatile) 1
    Polymer 74
    Salt 99

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    TOP96 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    		pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    220 μl of water (100 v/v)
    A2
    • Ammonium sulfate 2 M -
    		NA
    666 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    A3
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate (2 M)
    500 μl of water (100 v/v)
    A4
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    A6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    A7
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    • Polyethylene glycol 400 2 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Polyethylene glycol 400 (50 w/v)
    666 μl of Ammonium sulfate (3 M)
    193 μl of water (100 v/v)
    A8
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    350 μl of tri-Sodium citrate (4 M)
    550 μl of water (100 v/v)
    A9
    • Sodium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    		pH 8.5
    100 μl of Sodium acetate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of water (100 v/v)
    A10
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 30 w/v -
    		pH 8.5
    40 μl of Lithium sulfate (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    260 μl of water (100 v/v)
    A11
    • Sodium formate 4 M -
    		NA
    571 μl of Sodium formate pH 7 (7 M)
    428 μl of water (100 v/v)
    A12
    • Magnesium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 6.5
    100 μl of Magnesium acetate (2 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B1
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 20,000 12 w/v -
    		pH 6.5
    400 μl of Polyethylene glycol 20,000 (30 w/v)
    100 μl of MES pH 6.5 (1 M)
    500 μl of water (100 v/v)
    B3
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    320 μl of water (100 v/v)
    B4
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 30 w/v -
    		pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    B5
    • Calcium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Calcium chloride (2 M)
    500 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 10,000 20 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 10,000 (50 w/v)
    500 μl of water (100 v/v)
    B7
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    28 μl of Sodium formate pH 7 (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    B8
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    B9
    • tri-Sodium citrate 1.6 M -
    		NA
    400 μl of tri-Sodium citrate (4 M)
    600 μl of water (100 v/v)
    B10
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 w/v -
    		pH 7.5
    100 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Polyethylene glycol 400 (50 w/v)
    240 μl of water (100 v/v)
    B11
    • Ammonium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Ammonium chloride pH 6.3 (1 M)
    400 μl of water (100 v/v)
    B12
    • Magnesium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium formate pH 5.9 (1 M)
    400 μl of water (100 v/v)
    C1
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 25 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    333 μl of water (100 v/v)
    C2
    • di-Sodium malonate 2.4 M -
    		NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    C3
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Lithium sulfate (5 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    360 μl of water (100 v/v)
    C4
    • Potassium Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of Potassium Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    C5
    • Ammonium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    		pH 6.5
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    233 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 2 M -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    C7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of water (100 v/v)
    C8
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 1,500 25 w/v -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    320 μl of water (100 v/v)
    C9
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    320 μl of water (100 v/v)
    C10
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    320 μl of water (100 v/v)
    C11
    • Sodium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 30 w/v -
    		pH 6.5
    100 μl of Sodium acetate (2 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of water (100 v/v)
    C12
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of water (100 v/v)
    D1
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 1.5 M -
    		pH 7.5
    300 μl of Lithium sulfate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    25 μl of tri-Sodium citrate (4 M)
    475 μl of water (100 v/v)
    D3
    • Polyethylene glycol 1,500 25 w/v -
    		NA
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    500 μl of water (100 v/v)
    D4
    • Potassium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    575 μl of water (100 v/v)
    D5
    • Sodium acetate 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 8,000 18 w/v -
    		pH 6.5
    100 μl of Sodium acetate (2 M)
    360 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    440 μl of water (100 v/v)
    D6
    • Lithium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Lithium sulfate (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    360 μl of water (100 v/v)
    D7
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 8,000 30 w/v -
    		NA
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    D8
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    		pH 6.5
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of water (100 v/v)
    D9
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    D10
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Lithium sulfate (5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    360 μl of water (100 v/v)
    D11
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol 4,000 8 w/v -
    		pH 4.6
    100 μl of Sodium acetate pH 4.6 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    740 μl of water (100 v/v)
    D12
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    E1
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    E2
    • Polyethylene glycol 3,350 25 w/v -
    		NA
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    E3
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 w/v -
    		pH 7.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol 400 (50 w/v)
    220 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    666 μl of Ammonium sulfate (3 M)
    233 μl of water (100 v/v)
    E5
    • Sodium formate 3.5 M -
    		NA
    500 μl of Sodium formate pH 7 (7 M)
    500 μl of water (100 v/v)
    E6
    • MES 0.1 M pH 6.5
    • Magnesium sulfate 1.6 M -
    		pH 6.5
    100 μl of MES pH 6.5 (1 M)
    800 μl of Magnesium sulfate (2 M)
    100 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Magnesium chloride (2.5 M)
    520 μl of water (100 v/v)
    E8
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 4,000 30 w/v -
    		NA
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    333 μl of water (100 v/v)
    E9
    • Potassium thiocyanate 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    		NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    12 μl of Potassium thiocyanate (8 M)
    387 μl of water (100 v/v)
    E10
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    E11
    • Sodium acetate 0.1 M pH 4.6
    • Sodium formate 2 M -
    		pH 4.6
    285 μl of Sodium formate pH 7 (7 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    614 μl of water (100 v/v)
    E12
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    F1
    • Potassium Sodium tartrate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium sulfate 2 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    666 μl of Ammonium sulfate (3 M)
    133 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of water (100 v/v)
    F2
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 7.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of water (100 v/v)
    F3
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    533 μl of water (100 v/v)
    F4
    • Sodium cacodylate 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    		pH 6.5
    250 μl of tri-Sodium citrate (4 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    650 μl of water (100 v/v)
    F5
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    F6
    • Ammonium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium nitrate pH 6.3 (10 M)
    580 μl of water (100 v/v)
    F7
    • Sodium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Sodium thiocyanate pH 6.9 (8 M)
    575 μl of water (100 v/v)
    F8
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of Potassium nitrate pH 6.9 (3 M)
    533 μl of water (100 v/v)
    F9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    F10
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium acetate (2 M)
    500 μl of water (100 v/v)
    F11
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of water (100 v/v)
    F12
    • Calcium chloride 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 w/v -
    		pH 4.6
    100 μl of Calcium chloride (2 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    300 μl of 2-methyl-2,4-pentanediol BCC (100 w/v)
    500 μl of water (100 v/v)
    G1
    • Calcium acetate 0.2 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 8,000 20 w/v -
    		pH 6
    200 μl of Calcium acetate (1 M)
    33 μl of MES pH 6 (3 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    366 μl of water (100 v/v)
    G2
    • Sodium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Sodium sulfate (1 M)
    400 μl of water (100 v/v)
    G3
    • Zinc sulfate 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    		pH 6.5
    5 μl of Zinc sulfate (2 M)
    100 μl of MES pH 6.5 (1 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    645 μl of water (100 v/v)
    G4
    • di-Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of di-Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    G5
    • Tacsimate 60 v/v -
    		NA
    600 μl of Tacsimate pH 7 (100 v/v)
    400 μl of water (100 v/v)
    G6
    • Ammonium sulfate 0.5 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Lithium sulfate 1 M -
    		pH 5.6
    200 μl of Lithium sulfate (5 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    166 μl of Ammonium sulfate (3 M)
    533 μl of water (100 v/v)
    G7
    • Polyethylene glycol 1,500 30 w/v -
    		NA
    600 μl of Polyethylene glycol 1,500 (50 w/v)
    400 μl of water (100 v/v)
    G8
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 8.5
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    420 μl of water (100 v/v)
    G9
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Ammonium tartrate (2 M)
    500 μl of water (100 v/v)
    G10
    • Sodium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of Sodium fluoride (0.8 M)
    350 μl of water (100 v/v)
    G11
    • Sodium chloride 0.2 M -
    • Sodium cacodylate 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    		pH 6.5
    40 μl of Sodium chloride (5 M)
    666 μl of Ammonium sulfate (3 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    193 μl of water (100 v/v)
    G12
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.6 M -
    		pH 7.5
    20 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    533 μl of Ammonium sulfate (3 M)
    346 μl of water (100 v/v)
    H1
    • Ammonium formate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 7.5
    20 μl of Ammonium formate pH 6.6 (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    480 μl of water (100 v/v)
    H2
    • tri-Lithium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of tri-Lithium citrate (1.5 M)
    466 μl of water (100 v/v)
    H3
    • Ammonium iodide 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Ammonium iodide (2 M)
    500 μl of water (100 v/v)
    H4
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 6.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    200 μl of water (100 v/v)
    H5
    • Polyethylene glycol 4,000 30 w/v -
    		NA
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of water (100 v/v)
    H6
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H7
    • Ammonium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Ammonium fluoride (10 M)
    580 μl of water (100 v/v)
    H8
    • Ammonium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 10,000 17 w/v -
    		pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    100 μl of Ammonium acetate (1 M)
    460 μl of water (100 v/v)
    H9
    • Calcium acetate 0.2 M -
    • Imidazole 0.1 M pH 8
    • Polyethylene glycol 8,000 10 w/v -
    		pH 8
    200 μl of Calcium acetate (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Imidazole pH 8 (1 M)
    500 μl of water (100 v/v)
    H10
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    		pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    H11
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    H12
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 4.3 M -
    		pH 7.5
    860 μl of Sodium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of water (100 v/v)