Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
ShotGun SG1
Barcodes
ShotGun SG1
No barcodes found.
Screen Structure
ShotGun SG1
Well Count | 96 |
---|---|
Number of distinct ingredients | 50 |
Internal diversity (score mean dist) | 0.908 |
Screen Composition
ingredient type | ShotGun SG1 |
---|---|
Buffer | 87 |
Complex ingredient | 1 |
Organic (non-volatile) | 4 |
Organic (volatile) | 1 |
Polymer | 74 |
Salt | 99 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 8.5 | 100.0 μl of Magnesium chloride (2 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
A2 |
| NA | 571.4 μl of Ammonium sulfate (3.5 M) 428.6 μl of water (100 v/v) |
A3 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Sodium acetate (3 M) 533.3 μl of water (100 v/v) |
A4 |
| pH 8.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v) |
A5 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 125.0 μl of tri-Sodium citrate (1.6 M) 475.0 μl of water (100 v/v) |
A6 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
A7 |
| pH 7.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Polyethylene glycol 400 (100 v/v) 308.6 μl of water (100 v/v) |
A8 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 875.0 μl of tri-Sodium citrate (1.6 M) 25.0 μl of water (100 v/v) |
A9 |
| pH 8.5 | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 66.7 μl of Sodium acetate (3 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 233.3 μl of water (100 v/v) |
A10 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 200.0 μl of water (100 v/v) |
A11 |
| NA | 571.4 μl of Sodium formate (7 M) 428.6 μl of water (100 v/v) |
A12 |
| pH 6.5 | 200.0 μl of Magnesium acetate (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 300.0 μl of water (100 v/v) |
B1 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
B2 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 20,000 (30 w/v) 500.0 μl of water (100 v/v) |
B3 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
B4 |
| pH 6.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 242.9 μl of water (100 v/v) |
B5 |
| NA | 100.0 μl of Calcium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 10,000 (50 w/v) 500.0 μl of water (100 v/v) |
B7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium formate (7 M) 571.4 μl of water (100 v/v) |
B8 |
| pH 5.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 342.9 μl of water (100 v/v) |
B9 |
| NA | 1000.0 μl of tri-Sodium citrate (1.6 M) |
B10 |
| pH 7.5 | 100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v) |
B11 |
| NA | 40.0 μl of Ammonium chloride (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v) |
B12 |
| NA | 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
C1 |
| pH 4.6 | 57.1 μl of Ammonium sulfate (3.5 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 342.9 μl of water (100 v/v) |
C2 |
| NA | 466.7 μl of di-Sodium malonate pH 7 (3 M) 533.3 μl of water (100 v/v) |
C3 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
C4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of Potassium Sodium tartrate (1.5 M) 466.7 μl of water (100 v/v) |
C5 |
| pH 6.5 | 57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 242.9 μl of water (100 v/v) |
C6 |
| pH 4.6 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 328.6 μl of water (100 v/v) |
C7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
C8 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
C9 |
| pH 8.5 | 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
C10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
C11 |
| pH 6.5 | 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 66.7 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 233.3 μl of water (100 v/v) |
C12 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Sodium acetate (3 M) 333.3 μl of water (100 v/v) |
D1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 750.0 μl of Lithium sulfate (2 M) 150.0 μl of water (100 v/v) |
D2 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v) |
D3 |
| NA | 500.0 μl of Polyethylene glycol 1,500 (50 w/v) 500.0 μl of water (100 v/v) |
D4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Potassium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 360.0 μl of Polyethylene glycol 8,000 (50 w/v) 66.7 μl of Sodium acetate (3 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 473.3 μl of water (100 v/v) |
D6 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
D7 |
| NA | 57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 342.9 μl of water (100 v/v) |
D8 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v) 500.0 μl of water (100 v/v) |
D9 |
| pH 4.6 | 57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 242.9 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
D11 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 740.0 μl of water (100 v/v) |
D12 |
| pH 6.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 328.6 μl of water (100 v/v) |
E1 |
| pH 5.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 328.6 μl of water (100 v/v) |
E2 |
| NA | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 500.0 μl of water (100 v/v) |
E4 |
| pH 7.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 328.6 μl of water (100 v/v) |
E5 |
| NA | 500.0 μl of Sodium formate (7 M) 500.0 μl of water (100 v/v) |
E6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 640.0 μl of Magnesium sulfate (2.5 M) 260.0 μl of water (100 v/v) |
E7 |
| NA | 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
E8 |
| NA | 57.1 μl of Ammonium sulfate (3.5 M) 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 342.9 μl of water (100 v/v) |
E9 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v) |
E10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate pH 7 (3 M) 533.3 μl of water (100 v/v) |
E11 |
| pH 4.6 | 100.0 μl of Sodium acetate pH 4.6 (1 M) 285.7 μl of Sodium formate (7 M) 614.3 μl of water (100 v/v) |
E12 |
| pH 7.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 342.9 μl of water (100 v/v) |
F1 |
| pH 5.6 | 571.4 μl of Ammonium sulfate (3.5 M) 133.3 μl of Potassium Sodium tartrate (1.5 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 195.2 μl of water (100 v/v) |
F2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Sodium acetate (3 M) 333.3 μl of water (100 v/v) |
F3 |
| NA | 57.1 μl of Ammonium sulfate (3.5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 542.9 μl of water (100 v/v) |
F4 |
| pH 6.5 | 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v) |
F5 |
| pH 6.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 342.9 μl of water (100 v/v) |
F6 |
| NA | 100.0 μl of Ammonium nitrate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
F8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate (3 M) 533.3 μl of water (100 v/v) |
F9 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
F10 |
| NA | 200.0 μl of Magnesium acetate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F11 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F12 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 100.0 μl of Sodium acetate pH 4.6 (1 M) 590.0 μl of water (100 v/v) |
G1 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 66.7 μl of Sodium acetate (3 M) 433.3 μl of water (100 v/v) |
G2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of Sodium sulfate (1 M) 400.0 μl of water (100 v/v) |
G3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 5.0 μl of Zinc sulfate (2 M) 645.0 μl of water (100 v/v) |
G4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Sodium tartrate (2 M) 500.0 μl of water (100 v/v) |
G5 |
| NA | 600.0 μl of Tacsimate pH 7 (100 v/v) 400.0 μl of water (100 v/v) |
G6 |
| pH 5.6 | 142.9 μl of Ammonium sulfate (3.5 M) 500.0 μl of Lithium sulfate (2 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 257.1 μl of water (100 v/v) |
G7 |
| NA | 600.0 μl of Polyethylene glycol 1,500 (50 w/v) 400.0 μl of water (100 v/v) |
G8 |
| pH 8.5 | 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
G9 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Ammonium tartrate (2 M) 500.0 μl of water (100 v/v) |
G10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of Sodium fluoride (0.8 M) 350.0 μl of water (100 v/v) |
G11 |
| pH 6.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 40.0 μl of Sodium chloride (5 M) 288.6 μl of water (100 v/v) |
G12 |
| pH 7.5 | 457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 20.0 μl of Sodium chloride (5 M) 422.9 μl of water (100 v/v) |
H1 |
| NA | 20.0 μl of Ammonium formate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
H2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of tri-Lithium citrate (1.5 M) 466.7 μl of water (100 v/v) |
H3 |
| NA | 100.0 μl of Ammonium iodide (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
H4 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Sodium acetate (3 M) 333.3 μl of water (100 v/v) |
H5 |
| NA | 600.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
H6 |
| pH 8.5 | 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
H7 |
| NA | 20.0 μl of Ammonium fluoride (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
H8 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 33.3 μl of Sodium acetate (3 M) 526.7 μl of water (100 v/v) |
H9 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 66.7 μl of Sodium acetate (3 M) 633.3 μl of water (100 v/v) |
H10 |
| pH 8.5 | 57.1 μl of Ammonium sulfate (3.5 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 342.9 μl of water (100 v/v) |
H11 |
| pH 9 | 100.0 μl of CHES pH 9 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 860.0 μl of Sodium chloride (5 M) 40.0 μl of water (100 v/v) |