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Supplemental Information

Screen Description

ShotGun SG1

Molecular Dimensions / ShotGun SG1
Created 2014-12-22.

Export

ShotGun SG1

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ShotGun SG1

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Screen Structure

ShotGun SG1

Well Count 96
Number of distinct ingredients 50
Internal diversity (score mean dist) 0.908

Screen Composition

More information about screen composition chart.
2025-08-16T06:55:24.416713 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type ShotGun SG1
Buffer 87
Complex ingredient 1
Organic (non-volatile) 4
Organic (volatile) 1
Polymer 74
Salt 99

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
ShotGun SG1 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A2
  • Ammonium sulfate 2 M -
NA
571.4 μl of Ammonium sulfate (3.5 M)
428.6 μl of water (100 v/v)
A3
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
533.3 μl of water (100 v/v)
A4
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
A6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
A7
  • Ammonium sulfate 2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 2 v/v -
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Polyethylene glycol 400 (100 v/v)
308.6 μl of water (100 v/v)
A8
  • tri-Sodium citrate 1.4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
233.3 μl of water (100 v/v)
A10
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 30 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
A11
  • Sodium formate 4 M -
NA
571.4 μl of Sodium formate (7 M)
428.6 μl of water (100 v/v)
A12
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 20 w/v -
pH 6.5
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 20,000 12 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 20,000 (30 w/v)
500.0 μl of water (100 v/v)
B3
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
B4
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
242.9 μl of water (100 v/v)
B5
  • Calcium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 10,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 10,000 (50 w/v)
500.0 μl of water (100 v/v)
B7
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
B8
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
B9
  • tri-Sodium citrate 1.6 M -
NA
1000.0 μl of tri-Sodium citrate (1.6 M)
B10
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
B11
  • Ammonium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
40.0 μl of Ammonium chloride (5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
560.0 μl of water (100 v/v)
B12
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C1
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 25 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
342.9 μl of water (100 v/v)
C2
  • di-Sodium malonate 1.4 M -
NA
466.7 μl of di-Sodium malonate pH 7 (3 M)
533.3 μl of water (100 v/v)
C3
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
C4
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
C5
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
242.9 μl of water (100 v/v)
C6
  • Ammonium sulfate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
328.6 μl of water (100 v/v)
C7
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
C8
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
C9
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
C10
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
C11
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 30 w/v -
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
233.3 μl of water (100 v/v)
C12
  • Sodium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
D1
  • Lithium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of Lithium sulfate (2 M)
150.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 1,500 25 w/v -
NA
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
D4
  • Potassium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
575.0 μl of water (100 v/v)
D5
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 8,000 18 w/v -
pH 6.5
360.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
473.3 μl of water (100 v/v)
D6
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
D7
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 8,000 30 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
342.9 μl of water (100 v/v)
D8
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
D9
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
pH 4.6
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
242.9 μl of water (100 v/v)
D10
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
D11
  • Sodium acetate 0.1 M pH 4.6
  • Polyethylene glycol 4,000 8 w/v -
pH 4.6
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
740.0 μl of water (100 v/v)
D12
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
328.6 μl of water (100 v/v)
E1
  • Ammonium sulfate 2 M -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
328.6 μl of water (100 v/v)
E2
  • Polyethylene glycol 3,350 25 w/v -
NA
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E3
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
328.6 μl of water (100 v/v)
E5
  • Sodium formate 3.5 M -
NA
500.0 μl of Sodium formate (7 M)
500.0 μl of water (100 v/v)
E6
  • Magnesium sulfate 1.6 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
640.0 μl of Magnesium sulfate (2.5 M)
260.0 μl of water (100 v/v)
E7
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
E8
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 4,000 30 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
342.9 μl of water (100 v/v)
E9
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
E10
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of di-Sodium malonate pH 7 (3 M)
533.3 μl of water (100 v/v)
E11
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Sodium acetate pH 4.6 (1 M)
285.7 μl of Sodium formate (7 M)
614.3 μl of water (100 v/v)
E12
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F1
  • Potassium Sodium tartrate 0.2 M -
  • Ammonium sulfate 2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
571.4 μl of Ammonium sulfate (3.5 M)
133.3 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
195.2 μl of water (100 v/v)
F2
  • Sodium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
F3
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
542.9 μl of water (100 v/v)
F4
  • tri-Sodium citrate 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
F5
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F6
  • Ammonium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Ammonium nitrate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F7
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
F8
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium nitrate (3 M)
533.3 μl of water (100 v/v)
F9
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
F10
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F11
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F12
  • Calcium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Calcium chloride (2 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
590.0 μl of water (100 v/v)
G1
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 8,000 20 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
433.3 μl of water (100 v/v)
G2
  • Sodium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
400.0 μl of water (100 v/v)
G3
  • Zinc sulfate 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
5.0 μl of Zinc sulfate (2 M)
645.0 μl of water (100 v/v)
G4
  • di-Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium tartrate (2 M)
500.0 μl of water (100 v/v)
G5
  • Tacsimate 60 v/v -
NA
600.0 μl of Tacsimate pH 7 (100 v/v)
400.0 μl of water (100 v/v)
G6
  • Ammonium sulfate 0.5 M -
  • Lithium sulfate 1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
142.9 μl of Ammonium sulfate (3.5 M)
500.0 μl of Lithium sulfate (2 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
257.1 μl of water (100 v/v)
G7
  • Polyethylene glycol 1,500 30 w/v -
NA
600.0 μl of Polyethylene glycol 1,500 (50 w/v)
400.0 μl of water (100 v/v)
G8
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 8,000 20 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G9
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
G10
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
G11
  • Sodium chloride 0.2 M -
  • Ammonium sulfate 2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
288.6 μl of water (100 v/v)
G12
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 1.6 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Sodium chloride (5 M)
422.9 μl of water (100 v/v)
H1
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H2
  • tri-Lithium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Lithium citrate (1.5 M)
466.7 μl of water (100 v/v)
H3
  • Ammonium iodide 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Ammonium iodide (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
H4
  • Sodium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
H5
  • Polyethylene glycol 4,000 30 w/v -
NA
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
H6
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H7
  • Ammonium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
H8
  • Sodium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
33.3 μl of Sodium acetate (3 M)
526.7 μl of water (100 v/v)
H9
  • Sodium acetate 0.2 M -
  • Imidazole 0.1 M pH 8
  • Polyethylene glycol 8,000 10 w/v -
pH 8
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
633.3 μl of water (100 v/v)
H10
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
H11
  • CHES 0.1 M pH 9
  • Polyethylene glycol 8,000 20 w/v -
pH 9
100.0 μl of CHES pH 9 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H12
  • Sodium chloride 4.3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
860.0 μl of Sodium chloride (5 M)
40.0 μl of water (100 v/v)