C6 - Lipidic-Sponge Phase Drop

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Supplemental Information

Screen Description

Molecular Dimensions / Lipidic-Sponge Phase Drop

Created 2015-02-05.

Rigaku Recipe XML

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Screen Structure

Well Count	48
Number of distinct ingredients	12
Internal diversity (Score mean dist)	0.7
Internal diversity (Ion mean dist)	0.69
Internal diversity (PEG mean dist)	0.69
Internal diversity (Known pH mean dist)	0.62
Internal diversity (Penalty mean dist)	0.74

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type Lipidic-Sponge Phase Drop

Buffer 48

Organic (non-volatile) 25

Polymer 48

Salt 51

ingredient type	Lipidic-Sponge Phase Drop
Buffer	48
Organic (non-volatile)	25
Polymer	48
Salt	51

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Lipidic-Sponge Phase Drop Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Monoolein 30 w/v - Polyethylene glycol 400 40 w/v - MES 0.03 M pH 5.5 🔍 ⚖️	pH 5.5	30.0 μl of MES pH 5.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 270.0 μl of water (100 v/v)
A2	Monoolein 30 w/v - Polyethylene glycol 400 40 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - MES 0.03 M pH 5.5 🔍 ⚖️	pH 5.5	15.0 μl of Magnesium chloride (2 M) 30.0 μl of MES pH 5.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
A3	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - MES 0.03 M pH 5.5 🔍 ⚖️	pH 5.5	30.0 μl of MES pH 5.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 270.0 μl of water (100 v/v)
A4	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 5.5 🔍 ⚖️	pH 5.5	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 5.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 5.5 🔍 ⚖️	pH 5.5	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 5.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
A6	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - MES 0.03 M pH 6 🔍 ⚖️	pH 6	30.0 μl of MES pH 6 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 270.0 μl of water (100 v/v)
A7	Monoolein 40 w/v - Polyethylene glycol 400 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 6 🔍 ⚖️	pH 6	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 6 (1 M) 400.0 μl of Monoolein (100 w/v) 300.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
A8	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - MES 0.03 M pH 6 🔍 ⚖️	pH 6	30.0 μl of MES pH 6 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 270.0 μl of water (100 v/v)
B1	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.04 M - Magnesium chloride 0.04 M - MES 0.04 M pH 6 🔍 ⚖️	pH 6	20.0 μl of Magnesium chloride (2 M) 40.0 μl of MES pH 6 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 8.0 μl of Sodium chloride (5 M) 232.0 μl of water (100 v/v)
B2	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - MES 0.03 M pH 6 🔍 ⚖️	pH 6	15.0 μl of Magnesium chloride (2 M) 30.0 μl of MES pH 6 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 6 🔍 ⚖️	pH 6	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 6 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
B4	Monoolein 40 w/v - Polyethylene glycol 400 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - MES 0.03 M pH 6.5 🔍 ⚖️	pH 6.5	15.0 μl of Magnesium chloride (2 M) 30.0 μl of MES pH 6.5 (1 M) 400.0 μl of Monoolein (100 w/v) 300.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
B5	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 6.5 🔍 ⚖️	pH 6.5	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 6.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
B6	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - MES 0.03 M pH 6.5 🔍 ⚖️	pH 6.5	30.0 μl of MES pH 6.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 270.0 μl of water (100 v/v)
B7	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.04 M - Magnesium chloride 0.04 M - MES 0.04 M pH 6.5 🔍 ⚖️	pH 6.5	20.0 μl of Magnesium chloride (2 M) 40.0 μl of MES pH 6.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 8.0 μl of Sodium chloride (5 M) 232.0 μl of water (100 v/v)
B8	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - MES 0.03 M pH 6.5 🔍 ⚖️	pH 6.5	30.0 μl of MES pH 6.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 270.0 μl of water (100 v/v)
C1	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - MES 0.03 M pH 6.5 🔍 ⚖️	pH 6.5	15.0 μl of Lithium sulfate (2 M) 30.0 μl of MES pH 6.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
C2	Monoolein 40 w/v - Polyethylene glycol 400 30 w/v - HEPES 0.03 M pH 7 🔍 ⚖️	pH 7	30.0 μl of HEPES pH 7 (1 M) 400.0 μl of Monoolein (100 w/v) 300.0 μl of Polyethylene glycol 400 (100 w/v) 270.0 μl of water (100 v/v)
C3	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 7 🔍 ⚖️	pH 7	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 30.0 μl of tris chloride BCC pH 7 (1 M) 270.0 μl of water (100 v/v)
C4	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 7 🔍 ⚖️	pH 7	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 30.0 μl of tris chloride BCC pH 7 (1 M) 270.0 μl of water (100 v/v)
C5	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - HEPES 0.03 M pH 7 🔍 ⚖️	pH 7	30.0 μl of HEPES pH 7 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 270.0 μl of water (100 v/v)
C6	Monoolein 60 w/v - Jeffamine® M-600 15 w/v - Ammonium sulfate 0.025 M - HEPES 0.025 M pH 7 🔍 ⚖️	pH 7	7.14 μl of Ammonium sulfate (3.5 M) 25.0 μl of HEPES pH 7 (1 M) 150.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 217.86 μl of water (100 v/v)
C7	Monoolein 60 w/v - Jeffamine® M-600 17.5 w/v - Ammonium sulfate 0.0225 M - HEPES 0.0225 M pH 7 🔍 ⚖️	pH 7	6.43 μl of Ammonium sulfate (3.5 M) 22.5 μl of HEPES pH 7 (1 M) 175.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 196.07 μl of water (100 v/v)
C8	Monoolein 60 w/v - Jeffamine® M-600 20 w/v - Ammonium sulfate 0.02 M - HEPES 0.02 M pH 7 🔍 ⚖️	pH 7	5.71 μl of Ammonium sulfate (3.5 M) 20.0 μl of HEPES pH 7 (1 M) 200.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 174.29 μl of water (100 v/v)
D1	Monoolein 40 w/v - Polyethylene glycol 400 30 w/v - HEPES 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	30.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Monoolein (100 w/v) 300.0 μl of Polyethylene glycol 400 (100 w/v) 270.0 μl of water (100 v/v)
D2	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - HEPES 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	30.0 μl of HEPES pH 7.5 (1 M) 15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
D3	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - HEPES 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	30.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 270.0 μl of water (100 v/v)
D4	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - HEPES 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	30.0 μl of HEPES pH 7.5 (1 M) 15.0 μl of Lithium sulfate (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
D5	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 30.0 μl of tris chloride BCC pH 7.5 (1 M) 270.0 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 7.5 🔍 ⚖️	pH 7.5	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 7.5 (1 M) 249.0 μl of water (100 v/v)
D7	Monoolein 60 w/v - Jeffamine® M-600 20 w/v - Ammonium sulfate 0.02 M - HEPES 0.02 M pH 7.5 🔍 ⚖️	pH 7.5	5.71 μl of Ammonium sulfate (3.5 M) 20.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 174.29 μl of water (100 v/v)
D8	Monoolein 40 w/v - Polyethylene glycol 400 30 w/v - HEPES 0.03 M pH 8 🔍 ⚖️	pH 8	30.0 μl of HEPES pH 8 (1 M) 400.0 μl of Monoolein (100 w/v) 300.0 μl of Polyethylene glycol 400 (100 w/v) 270.0 μl of water (100 v/v)
E1	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - HEPES 0.03 M pH 8 🔍 ⚖️	pH 8	30.0 μl of HEPES pH 8 (1 M) 15.0 μl of Lithium sulfate (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 249.0 μl of water (100 v/v)
E2	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 8 🔍 ⚖️	pH 8	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 8 (1 M) 249.0 μl of water (100 v/v)
E3	Monoolein 60 w/v - Jeffamine® M-600 15 w/v - Ammonium sulfate 0.025 M - HEPES 0.025 M pH 8 🔍 ⚖️	pH 8	7.14 μl of Ammonium sulfate (3.5 M) 25.0 μl of HEPES pH 8 (1 M) 150.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 217.86 μl of water (100 v/v)
E4	Monoolein 60 w/v - Jeffamine® M-600 17.5 w/v - Ammonium sulfate 0.0225 M - HEPES 0.0225 M pH 8 🔍 ⚖️	pH 8	6.43 μl of Ammonium sulfate (3.5 M) 22.5 μl of HEPES pH 8 (1 M) 175.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 196.07 μl of water (100 v/v)
E5	Monoolein 60 w/v - Jeffamine® M-600 20 w/v - Ammonium sulfate 0.02 M - HEPES 0.02 M pH 8 🔍 ⚖️	pH 8	5.71 μl of Ammonium sulfate (3.5 M) 20.0 μl of HEPES pH 8 (1 M) 200.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 174.29 μl of water (100 v/v)
E6	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 30.0 μl of tris chloride BCC pH 8.5 (1 M) 270.0 μl of water (100 v/v)
E7	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 8.5 🔍 ⚖️	pH 8.5	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 8.5 (1 M) 249.0 μl of water (100 v/v)
E8	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 8.5 🔍 ⚖️	pH 8.5	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 8.5 (1 M) 249.0 μl of water (100 v/v)
F1	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 30.0 μl of tris chloride BCC pH 8.5 (1 M) 270.0 μl of water (100 v/v)
F2	Monoolein 60 w/v - Jeffamine® M-600 15 w/v - Ammonium sulfate 0.025 M - HEPES 0.025 M pH 8.5 🔍 ⚖️	pH 8.5	7.14 μl of Ammonium sulfate (3.5 M) 25.0 μl of HEPES pH 8.5 (1 M) 150.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 217.86 μl of water (100 v/v)
F3	Monoolein 60 w/v - Jeffamine® M-600 17.5 w/v - Ammonium sulfate 0.0225 M - HEPES 0.0225 M pH 8.5 🔍 ⚖️	pH 8.5	6.43 μl of Ammonium sulfate (3.5 M) 22.5 μl of HEPES pH 8.5 (1 M) 175.0 μl of Jeffamine® M-600 (100 w/v) 600.0 μl of Monoolein (100 w/v) 196.07 μl of water (100 v/v)
F4	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 9 🔍 ⚖️	pH 9	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 30.0 μl of tris chloride BCC pH 9 (1 M) 270.0 μl of water (100 v/v)
F5	Polyethylene glycol 400 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 9 🔍 ⚖️	pH 9	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 400 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 9 (1 M) 249.0 μl of water (100 v/v)
F6	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - tris chloride BCC 0.03 M pH 9 🔍 ⚖️	pH 9	300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 30.0 μl of tris chloride BCC pH 9 (1 M) 270.0 μl of water (100 v/v)
F7	Polyethylene glycol 1,500 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Magnesium chloride 0.03 M - tris chloride BCC 0.03 M pH 9 🔍 ⚖️	pH 9	15.0 μl of Magnesium chloride (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 1,500 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 9 (1 M) 249.0 μl of water (100 v/v)
F8	Polyethylene glycol 4,000 40 w/v - Monoolein 30 w/v - Sodium chloride 0.03 M - Lithium sulfate 0.03 M - tris chloride BCC 0.03 M pH 9 🔍 ⚖️	pH 9	15.0 μl of Lithium sulfate (2 M) 300.0 μl of Monoolein (100 w/v) 400.0 μl of Polyethylene glycol 4,000 (100 w/v) 6.0 μl of Sodium chloride (5 M) 30.0 μl of tris chloride BCC pH 9 (1 M) 249.0 μl of water (100 v/v)