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Supplemental Information

Screen Description

Lipidic-Sponge Phase Drop

Molecular Dimensions / Lipidic-Sponge Phase Drop
Created 2015-02-05.

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Lipidic-Sponge Phase Drop

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Lipidic-Sponge Phase Drop

    Screen Structure

    Lipidic-Sponge Phase Drop

    Well Count 48
    Number of distinct ingredients 12
    Internal diversity (score mean dist) 0.699

    Screen Composition

    More information about screen composition chart.
    2025-06-13T21:15:36.978960 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Lipidic-Sponge Phase Drop
    Buffer 48
    Organic (non-volatile) 25
    Polymer 48
    Salt 51

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Lipidic-Sponge Phase Drop Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • MES 0.03 M pH 5.5
    • Monoolein 30 w/v -
    • Polyethylene glycol 400 40 w/v -
    		pH 5.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 400 (100 w/v)
    30 μl of MES pH 5.5 (1 M)
    270 μl of water (100 v/v)
    A2
    • MES 0.03 M pH 5.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    • Monoolein 30 w/v -
    • Polyethylene glycol 400 40 w/v -
    		pH 5.5
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    6 μl of Sodium chloride (5 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    30 μl of MES pH 5.5 (1 M)
    249 μl of water (100 v/v)
    A3
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 5.5
    		pH 5.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    30 μl of MES pH 5.5 (1 M)
    270 μl of water (100 v/v)
    A4
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 5.5
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 5.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    6 μl of Sodium chloride (5 M)
    15 μl of Lithium sulfate (2 M)
    30 μl of MES pH 5.5 (1 M)
    249 μl of water (100 v/v)
    A5
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 5.5
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 5.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    6 μl of Sodium chloride (5 M)
    15 μl of Lithium sulfate (2 M)
    30 μl of MES pH 5.5 (1 M)
    249 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6
    		pH 6
    300 μl of Monoolein (100 w/v)
    30 μl of MES pH 6 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    A7
    • Polyethylene glycol 400 30 w/v -
    • Monoolein 40 w/v -
    • MES 0.03 M pH 6
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 6
    400 μl of Monoolein (100 w/v)
    30 μl of MES pH 6 (1 M)
    6 μl of Sodium chloride (5 M)
    15 μl of Lithium sulfate (2 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    A8
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6
    		pH 6
    300 μl of Monoolein (100 w/v)
    30 μl of MES pH 6 (1 M)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    270 μl of water (100 v/v)
    B1
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.04 M pH 6
    • Sodium chloride 0.04 M -
    • Magnesium chloride 0.04 M -
    		pH 6
    300 μl of Monoolein (100 w/v)
    20 μl of Magnesium chloride (2 M)
    40 μl of MES pH 6 (1 M)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    8 μl of Sodium chloride (5 M)
    232 μl of water (100 v/v)
    B2
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 6
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    15 μl of Magnesium chloride (2 M)
    30 μl of MES pH 6 (1 M)
    6 μl of Sodium chloride (5 M)
    249 μl of water (100 v/v)
    B3
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 6
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    30 μl of MES pH 6 (1 M)
    6 μl of Sodium chloride (5 M)
    15 μl of Lithium sulfate (2 M)
    249 μl of water (100 v/v)
    B4
    • Polyethylene glycol 400 30 w/v -
    • Monoolein 40 w/v -
    • MES 0.03 M pH 6.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 6.5
    400 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    6 μl of Sodium chloride (5 M)
    30 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    B5
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6.5
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 6.5
    300 μl of Monoolein (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of MES pH 6.5 (1 M)
    15 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    B6
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6.5
    		pH 6.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    30 μl of MES pH 6.5 (1 M)
    270 μl of water (100 v/v)
    B7
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.04 M pH 6.5
    • Sodium chloride 0.04 M -
    • Magnesium chloride 0.04 M -
    		pH 6.5
    300 μl of Monoolein (100 w/v)
    20 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    8 μl of Sodium chloride (5 M)
    40 μl of MES pH 6.5 (1 M)
    232 μl of water (100 v/v)
    B8
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6.5
    		pH 6.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    30 μl of MES pH 6.5 (1 M)
    270 μl of water (100 v/v)
    C1
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • MES 0.03 M pH 6.5
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 6.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of MES pH 6.5 (1 M)
    15 μl of Lithium sulfate (2 M)
    249 μl of water (100 v/v)
    C2
    • Polyethylene glycol 400 30 w/v -
    • Monoolein 40 w/v -
    • HEPES 0.03 M pH 7
    		pH 7
    400 μl of Monoolein (100 w/v)
    30 μl of HEPES pH 7 (1 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 7
    		pH 7
    300 μl of Monoolein (100 w/v)
    30 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    C4
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 7
    		pH 7
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    30 μl of tris chloride BCC pH 7 (1 M)
    270 μl of water (100 v/v)
    C5
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • HEPES 0.03 M pH 7
    		pH 7
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    30 μl of HEPES pH 7 (1 M)
    270 μl of water (100 v/v)
    C6
    • Jeffamine® M-600 15 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.025 M pH 7
    • Ammonium sulfate 0.025 M -
    		pH 7
    7 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    150 μl of Jeffamine® M-600 (100 w/v)
    25 μl of HEPES pH 7 (1 M)
    217 μl of water (100 v/v)
    C7
    • Jeffamine® M-600 17.5 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.0225 M pH 7
    • Ammonium sulfate 0.0225 M -
    		pH 7
    6 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    175 μl of Jeffamine® M-600 (100 w/v)
    22 μl of HEPES pH 7 (1 M)
    196 μl of water (100 v/v)
    C8
    • Jeffamine® M-600 20 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.02 M pH 7
    • Ammonium sulfate 0.02 M -
    		pH 7
    5 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    200 μl of Jeffamine® M-600 (100 w/v)
    20 μl of HEPES pH 7 (1 M)
    174 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 30 w/v -
    • Monoolein 40 w/v -
    • HEPES 0.03 M pH 7.5
    		pH 7.5
    400 μl of Monoolein (100 w/v)
    30 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • HEPES 0.03 M pH 7.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 7.5
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    6 μl of Sodium chloride (5 M)
    30 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    D3
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • HEPES 0.03 M pH 7.5
    		pH 7.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    30 μl of HEPES pH 7.5 (1 M)
    270 μl of water (100 v/v)
    D4
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • HEPES 0.03 M pH 7.5
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 7.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of HEPES pH 7.5 (1 M)
    15 μl of Lithium sulfate (2 M)
    249 μl of water (100 v/v)
    D5
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 7.5
    		pH 7.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    30 μl of tris chloride BCC pH 7.5 (1 M)
    270 μl of water (100 v/v)
    D6
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 7.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 7.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    15 μl of Magnesium chloride (2 M)
    30 μl of tris chloride BCC pH 7.5 (1 M)
    6 μl of Sodium chloride (5 M)
    249 μl of water (100 v/v)
    D7
    • Jeffamine® M-600 20 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.02 M pH 7.5
    • Ammonium sulfate 0.02 M -
    		pH 7.5
    5 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    200 μl of Jeffamine® M-600 (100 w/v)
    20 μl of HEPES pH 7.5 (1 M)
    174 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 30 w/v -
    • Monoolein 40 w/v -
    • HEPES 0.03 M pH 8
    		pH 8
    400 μl of Monoolein (100 w/v)
    30 μl of HEPES pH 8 (1 M)
    300 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    E1
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • HEPES 0.03 M pH 8
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 8
    300 μl of Monoolein (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of HEPES pH 8 (1 M)
    15 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    E2
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 8
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 8
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    15 μl of Magnesium chloride (2 M)
    30 μl of tris chloride BCC pH 8 (1 M)
    6 μl of Sodium chloride (5 M)
    249 μl of water (100 v/v)
    E3
    • Jeffamine® M-600 15 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.025 M pH 8
    • Ammonium sulfate 0.025 M -
    		pH 8
    7 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    150 μl of Jeffamine® M-600 (100 w/v)
    25 μl of HEPES pH 8 (1 M)
    217 μl of water (100 v/v)
    E4
    • Jeffamine® M-600 17.5 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.0225 M pH 8
    • Ammonium sulfate 0.0225 M -
    		pH 8
    6 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    175 μl of Jeffamine® M-600 (100 w/v)
    22 μl of HEPES pH 8 (1 M)
    196 μl of water (100 v/v)
    E5
    • Jeffamine® M-600 20 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.02 M pH 8
    • Ammonium sulfate 0.02 M -
    		pH 8
    5 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    200 μl of Jeffamine® M-600 (100 w/v)
    20 μl of HEPES pH 8 (1 M)
    174 μl of water (100 v/v)
    E6
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 8.5
    		pH 8.5
    300 μl of Monoolein (100 w/v)
    30 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    E7
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 8.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 8.5
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    6 μl of Sodium chloride (5 M)
    30 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    E8
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 8.5
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 8.5
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of tris chloride BCC pH 8.5 (1 M)
    249 μl of water (100 v/v)
    F1
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 8.5
    		pH 8.5
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    30 μl of tris chloride BCC pH 8.5 (1 M)
    270 μl of water (100 v/v)
    F2
    • Jeffamine® M-600 15 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.025 M pH 8.5
    • Ammonium sulfate 0.025 M -
    		pH 8.5
    7 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    25 μl of HEPES pH 8.5 (1 M)
    150 μl of Jeffamine® M-600 (100 w/v)
    217 μl of water (100 v/v)
    F3
    • Jeffamine® M-600 17.5 w/v -
    • Monoolein 60 w/v -
    • HEPES 0.0225 M pH 8.5
    • Ammonium sulfate 0.0225 M -
    		pH 8.5
    6 μl of Ammonium sulfate (3.5 M)
    600 μl of Monoolein (100 w/v)
    22 μl of HEPES pH 8.5 (1 M)
    175 μl of Jeffamine® M-600 (100 w/v)
    196 μl of water (100 v/v)
    F4
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 9
    		pH 9
    300 μl of Monoolein (100 w/v)
    30 μl of tris chloride BCC pH 9 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    270 μl of water (100 v/v)
    F5
    • Polyethylene glycol 400 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 9
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 9
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    6 μl of Sodium chloride (5 M)
    30 μl of tris chloride BCC pH 9 (1 M)
    400 μl of Polyethylene glycol 400 (100 w/v)
    249 μl of water (100 v/v)
    F6
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 9
    		pH 9
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    30 μl of tris chloride BCC pH 9 (1 M)
    270 μl of water (100 v/v)
    F7
    • Polyethylene glycol 1,500 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 9
    • Sodium chloride 0.03 M -
    • Magnesium chloride 0.03 M -
    		pH 9
    300 μl of Monoolein (100 w/v)
    15 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 1,500 (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of tris chloride BCC pH 9 (1 M)
    249 μl of water (100 v/v)
    F8
    • Polyethylene glycol 4,000 40 w/v -
    • Monoolein 30 w/v -
    • tris chloride BCC 0.03 M pH 9
    • Sodium chloride 0.03 M -
    • Lithium sulfate 0.03 M -
    		pH 9
    300 μl of Monoolein (100 w/v)
    400 μl of Polyethylene glycol 4,000 (100 w/v)
    6 μl of Sodium chloride (5 M)
    30 μl of tris chloride BCC pH 9 (1 M)
    15 μl of Lithium sulfate (2 M)
    249 μl of water (100 v/v)