Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

LCP HTS

LCP / LCP HTS
Created 2016-09-29.

Export

Barcodes

LCP HTS

    Screen Structure

    LCP HTS

    Well Count 96
    Number of distinct ingredients 75
    Internal diversity (score mean dist) 0.884

    Screen Composition

    2025-06-14T13:05:47.773839 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type LCP HTS
    Buffer 132
    Cryoprotectant 1
    Organic (non-volatile) 83
    Organic (volatile) 5
    Polymer 88
    Salt 132

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    LCP HTS Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 300 15 v/v -
    • HEPES 0.1 M pH 7.6
    • Ethylenediaminetetraacetic acid disodium salt 0.05 M -
    • 1,7 Heptanediol 1.2 v/v -
    pH 7.6
    100 μl of Ethylenediaminetetraacetic acid disodium salt pH 8 (0.5 M)
    12 μl of 1,7 Heptanediol (100 v/v)
    150 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 7.6 (1 M)
    638 μl of water (100 v/v)
    A2
    • Polyethylene glycol 300 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium di-hydrogen phosphate 0.1 M -
    • 2-methyl-2,4-pentanediol BCC 2 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Ammonium di-hydrogen phosphate (1 M)
    20 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    300 μl of Polyethylene glycol 300 (100 v/v)
    480 μl of water (100 v/v)
    A3
    • Polyethylene glycol 300 30 v/v -
    • Sodium chloride 0.15 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 6.3
    pH 6.3
    150 μl of Sodium chloride (1 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium di-hydrogen phosphate pH 6.3 (1 M)
    450 μl of water (100 v/v)
    A4
    • Polyethylene glycol 300 32 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium di-hydrogen phosphate 0.1 M -
    • 1,7 Heptanediol 1 v/v -
    pH 7.5
    10 μl of 1,7 Heptanediol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Ammonium di-hydrogen phosphate (1 M)
    320 μl of Polyethylene glycol 300 (100 v/v)
    470 μl of water (100 v/v)
    A5
    • Polyethylene glycol 300 37 v/v -
    • BIS-TRIS propane 0.1 M pH 6.5
    • di-Ammonium hydrogen phosphate 0.1 M -
    pH 6.5
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of BIS-TRIS propane pH 6.5 (1 M)
    370 μl of Polyethylene glycol 300 (100 v/v)
    501 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 10 v/v -
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.15 M -
    pH 7.5
    100 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    150 μl of Ammonium sulfate (1 M)
    650 μl of water (100 v/v)
    A7
    • Polyethylene glycol 400 13 v/v -
    • MES 0.1 M pH 6
    • Potassium Sodium tartrate 0.17 M -
    • Jeffamine® M-600 0.45 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    130 μl of Polyethylene glycol 400 (100 v/v)
    9 μl of Jeffamine® M-600 pH 7 (50 v/v)
    170 μl of Potassium Sodium tartrate (1 M)
    591 μl of water (100 v/v)
    A8
    • Polyethylene glycol 400 14 v/v -
    • tris chloride BCC 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 6 v/v -
    pH 7.5
    140 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    60 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    700 μl of water (100 v/v)
    A9
    • Polyethylene glycol 400 18 v/v -
    • tris chloride BCC 0.1 M pH 7.5
    • tri-Sodium citrate 0.1 M -
    pH 7.5
    180 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of tri-Sodium citrate (4 M)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    695 μl of water (100 v/v)
    A10
    • Polyethylene glycol 400 18 v/v -
    • HEPES 0.05 M pH 7
    • Potassium sulfate 0.1 M -
    pH 7
    142 μl of Potassium sulfate (0.7 M)
    180 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of HEPES pH 7 (1 M)
    627 μl of water (100 v/v)
    A11
    • Polyethylene glycol 400 18.5 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Lithium sulfate 0.05 M -
    • Sodium chloride 0.1 M -
    pH 5
    185 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (1 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    16 μl of Lithium sulfate (3 M)
    598 μl of water (100 v/v)
    A12
    • Polyethylene glycol 400 19.5 v/v -
    • HEPES 0.1 M pH 7
    • Ammonium di-hydrogen phosphate 0.35 M -
    pH 7
    195 μl of Polyethylene glycol 400 (100 v/v)
    116 μl of Ammonium di-hydrogen phosphate (3 M)
    100 μl of HEPES pH 7 (1 M)
    588 μl of water (100 v/v)
    B1
    • Polyethylene glycol 400 20 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • di-Sodium malonate 0.3 M -
    • 2-Aminoethanesulfonic acid 0.005 M -
    pH 5.5
    200 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    50 μl of 2-Aminoethanesulfonic acid (0.1 M)
    100 μl of di-Sodium malonate (3 M)
    550 μl of water (100 v/v)
    B2
    • Polyethylene glycol 400 20 v/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.4 M -
    pH 7
    200 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    300 μl of water (100 v/v)
    B3
    • Polyethylene glycol 400 20 v/v -
    • MES 0.1 M pH 6.5
    • Potassium nitrate 0.4 M -
    • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
    pH 6.5
    200 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    133 μl of Potassium nitrate pH 6.9 (3 M)
    1 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
    565 μl of water (100 v/v)
    B4
    • Polyethylene glycol 400 21 v/v -
    • MES 0.1 M pH 6.5
    • di-Ammonium hydrogen phosphate 0.07 M -
    pH 6.5
    210 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    20 μl of di-Ammonium hydrogen phosphate (3.5 M)
    670 μl of water (100 v/v)
    B5
    • Polyethylene glycol 400 23 v/v -
    • di-Sodium malonate 0.3 M -
    • Nickel (II) chloride 0.005 M -
    • tri-Sodium citrate 0.1 M pH 5.2
    pH 5.2
    230 μl of Polyethylene glycol 400 (100 v/v)
    5 μl of Nickel (II) chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.2 (1 M)
    150 μl of di-Sodium malonate (2 M)
    515 μl of water (100 v/v)
    B6
    • Polyethylene glycol 400 25 v/v -
    • HEPES 0.1 M pH 7.5
    • Potassium Sodium tartrate 0.2 M -
    pH 7.5
    250 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Potassium Sodium tartrate (1 M)
    450 μl of water (100 v/v)
    B7
    • Polyethylene glycol 400 26 v/v -
    • Lithium sulfate 0.2 M -
    • 1,4-Butanediol 3.5 v/v -
    • Dimethyl sulfoxide 4 v/v -
    • MES 0.1 M pH 6.7
    pH 6.7
    260 μl of Polyethylene glycol 400 (100 v/v)
    35 μl of 1,4-Butanediol (100 v/v)
    40 μl of Dimethyl sulfoxide (100 v/v)
    100 μl of MES pH 6.7 (1 M)
    100 μl of Lithium sulfate (2 M)
    465 μl of water (100 v/v)
    B8
    • Polyethylene glycol 400 26 v/v -
    • MES 0.1 M pH 6
    • di-Sodium malonate 0.3 M -
    • Strontium chloride 0.005 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Sodium malonate (3 M)
    50 μl of Strontium chloride (0.1 M)
    490 μl of water (100 v/v)
    B9
    • Polyethylene glycol 400 26 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Ammonium sulfate 0.3 M -
    pH 8
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium sulfate (3 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    540 μl of water (100 v/v)
    B10
    • Polyethylene glycol 400 26.5 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Sodium thiocyanate 0.05 M -
    • 2,5-Hexanediol 2 v/v -
    pH 5
    265 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    25 μl of Sodium thiocyanate (2 M)
    50 μl of 2,5-Hexanediol (40 v/v)
    560 μl of water (100 v/v)
    B11
    • Polyethylene glycol 400 27 v/v -
    • Sodium formate 0.22 M -
    • 1,4-Butanediol 5 v/v -
    • tris chloride BCC 0.1 M pH 7.8
    pH 7.8
    270 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 7.8 (1 M)
    50 μl of 1,4-Butanediol (100 v/v)
    44 μl of Sodium formate (5 M)
    536 μl of water (100 v/v)
    B12
    • Polyethylene glycol 400 27 v/v -
    • Ammonium fluoride 0.15 M -
    • Jeffamine® M-600 2.5 v/v -
    • HEPES 0.1 M pH 6.9
    pH 6.9
    270 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 6.9 (1 M)
    15 μl of Ammonium fluoride (10 M)
    50 μl of Jeffamine® M-600 (50 v/v)
    565 μl of water (100 v/v)
    C1
    • Polyethylene glycol 400 27.5 v/v -
    • di-Sodium tartrate 0.12 M -
    • 1,3-Butanediol 3 v/v -
    • BIS-TRIS propane 0.1 M pH 6.7
    pH 6.7
    275 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 6.7 (1 M)
    60 μl of di-Sodium tartrate (2 M)
    75 μl of 1,3-Butanediol (40 v/v)
    490 μl of water (100 v/v)
    C2
    • Polyethylene glycol 400 27.5 v/v -
    • Potassium Sodium tartrate 0.15 M -
    • BIS-TRIS propane 0.1 M pH 6.4
    pH 6.4
    275 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 6.4 (1 M)
    150 μl of Potassium Sodium tartrate (1 M)
    475 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 28 v/v -
    • BIS-TRIS propane 0.1 M pH 7
    • Potassium formate 0.3 M -
    pH 7
    280 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 7 (1 M)
    21 μl of Potassium formate (14 M)
    598 μl of water (100 v/v)
    C4
    • Polyethylene glycol 400 28 v/v -
    • tri-Sodium citrate 0.1 M pH 4.5
    • Ammonium di-hydrogen phosphate 0.3 M -
    • Magnesium chloride 0.01 M -
    pH 4.5
    280 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 4.5 (1 M)
    4 μl of Magnesium chloride (2.5 M)
    300 μl of Ammonium di-hydrogen phosphate (1 M)
    316 μl of water (100 v/v)
    C5
    • Polyethylene glycol 400 28.5 v/v -
    • HEPES 0.1 M pH 7
    • di-Ammonium hydrogen phosphate 0.1 M -
    • Tris(2-carboxyethyl)phosphine hydrochloride 0.006 M -
    pH 7
    285 μl of Polyethylene glycol 400 (100 v/v)
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    6 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    580 μl of water (100 v/v)
    C6
    • Polyethylene glycol 400 29 v/v -
    • di-Ammonium hydrogen phosphate 0.2 M -
    • MES 0.1 M pH 6.8
    pH 6.8
    290 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.8 (1 M)
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    552 μl of water (100 v/v)
    C7
    • Polyethylene glycol 400 29 v/v -
    • tri-Sodium citrate 0.05 M pH 4
    • Lithium sulfate 0.2 M -
    pH 4
    50 μl of tri-Sodium citrate pH 4 (1 M)
    290 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate (2 M)
    560 μl of water (100 v/v)
    C8
    • Polyethylene glycol 400 29.5 v/v -
    • Sodium formate 0.35 M -
    • 1,4-Butanediol 5 v/v -
    • tris chloride BCC 0.1 M pH 7.8
    pH 7.8
    295 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 7.8 (1 M)
    50 μl of 1,4-Butanediol (100 v/v)
    50 μl of Sodium formate (7 M)
    505 μl of water (100 v/v)
    C9
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium malonate 0.2 M -
    pH 8
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of di-Sodium malonate (2 M)
    500 μl of water (100 v/v)
    C10
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.1 M pH 6.5
    • Lithium sulfate 0.185 M -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 6.5 (1 M)
    92 μl of Lithium sulfate (2 M)
    507 μl of water (100 v/v)
    C11
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Magnesium chloride 0.2 M -
    pH 5
    300 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    520 μl of water (100 v/v)
    C12
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Lithium sulfate 0.2 M -
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    500 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 30 v/v -
    • BIS-TRIS propane 0.1 M pH 7.5
    • Ammonium acetate 0.3 M -
    • D-(+)-Glucose 2 w/v -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    66 μl of D-(+)-Glucose (30 w/v)
    100 μl of BIS-TRIS propane pH 7.5 (1 M)
    60 μl of Ammonium acetate (5 M)
    473 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.1 M -
    pH 7
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    500 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6
    • Magnesium sulfate 0.1 M -
    • Polypropylene glycol 400 2.5 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of Magnesium sulfate (1 M)
    475 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 30 v/v -
    • Potassium nitrate 0.4 M -
    • tri-Sodium citrate 0.1 M pH 6
    pH 6
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 6 (1 M)
    400 μl of Potassium nitrate (1 M)
    200 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.1 M pH 7.5
    • Lithium chloride 0.4 M -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    400 μl of Lithium chloride (1 M)
    200 μl of water (100 v/v)
    D6
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Magnesium sulfate 0.1 M -
    pH 8
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Magnesium sulfate (1 M)
    500 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 30 v/v -
    • Ammonium formate 0.1 M -
    • MES 0.1 M pH 6.3
    pH 6.3
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.3 (1 M)
    20 μl of Ammonium formate (5 M)
    580 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7
    • Sodium thiocyanate 0.1 M -
    pH 7
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium thiocyanate (2 M)
    100 μl of HEPES pH 7 (1 M)
    550 μl of water (100 v/v)
    D9
    • Polyethylene glycol 400 30 v/v -
    • MES 0.1 M pH 6.5
    • Sodium thiocyanate 0.1 M -
    • Calcium chloride 0.02 M -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    50 μl of Sodium thiocyanate (2 M)
    20 μl of Calcium chloride (1 M)
    530 μl of water (100 v/v)
    D10
    • Polyethylene glycol 400 30 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Sodium thiocyanate 0.1 M -
    • Calcium chloride 0.02 M -
    pH 8
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium thiocyanate (2 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    20 μl of Calcium chloride (1 M)
    530 μl of water (100 v/v)
    D11
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    • D-(+)-Trehalose 3 w/v -
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    100 μl of D-(+)-Trehalose (30 w/v)
    400 μl of water (100 v/v)
    D12
    • Polyethylene glycol 400 30 v/v -
    • MES 0.05 M pH 6.5
    • Magnesium chloride 0.1 M -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of MES pH 6.5 (1 M)
    40 μl of Magnesium chloride (2.5 M)
    610 μl of water (100 v/v)
    E1
    • Polyethylene glycol 400 30 v/v -
    • HEPES 0.1 M pH 7.5
    • ß-Mercaptoethanol 0.002 M -
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    2 μl of ß-Mercaptoethanol (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    598 μl of water (100 v/v)
    E2
    • Polyethylene glycol 400 31 v/v -
    • MES 0.1 M pH 6
    • di-Sodium malonate 0.3 M -
    • Cobalt (III) Hexamine chloride 0.005 M -
    pH 6
    100 μl of MES pH 6 (1 M)
    310 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Cobalt (III) Hexamine chloride (0.1 M)
    100 μl of di-Sodium malonate (3 M)
    440 μl of water (100 v/v)
    E3
    • Polyethylene glycol 400 31 v/v -
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 0.15 M -
    • Magnesium chloride 0.35 M -
    pH 7.5
    310 μl of Polyethylene glycol 400 (100 v/v)
    140 μl of Magnesium chloride (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    75 μl of tri-Sodium citrate (2 M)
    375 μl of water (100 v/v)
    E4
    • Polyethylene glycol 400 32 v/v -
    • HEPES 0.1 M pH 7.8
    • Ammonium fluoride 0.07 M -
    • Polypropylene glycol 400 6 v/v -
    pH 7.8
    100 μl of HEPES pH 7.8 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    60 μl of Polypropylene glycol 400 (100 v/v)
    7 μl of Ammonium fluoride (10 M)
    513 μl of water (100 v/v)
    E5
    • Polyethylene glycol 400 32 v/v -
    • HEPES 0.1 M pH 7
    • Sodium chloride 0.1 M -
    pH 7
    320 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    480 μl of water (100 v/v)
    E6
    • Polyethylene glycol 400 32 v/v -
    • MES 0.05 M pH 6.5
    • Sodium thiocyanate 0.1 M -
    • Calcium chloride 0.02 M -
    pH 6.5
    320 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of MES pH 6.5 (1 M)
    50 μl of Sodium thiocyanate (2 M)
    20 μl of Calcium chloride (1 M)
    560 μl of water (100 v/v)
    E7
    • Polyethylene glycol 400 32 v/v -
    • tri-Sodium citrate 0.1 M pH 4.5
    • Sodium chloride 0.075 M -
    • Magnesium chloride 0.13 M -
    pH 4.5
    320 μl of Polyethylene glycol 400 (100 v/v)
    75 μl of Sodium chloride (1 M)
    100 μl of tri-Sodium citrate pH 4.5 (1 M)
    52 μl of Magnesium chloride (2.5 M)
    453 μl of water (100 v/v)
    E8
    • Polyethylene glycol 400 32.5 v/v -
    • Sodium sulfate 0.15 M -
    • 1,4-Butanediol 6 v/v -
    • BIS-TRIS propane 0.1 M pH 6.8
    pH 6.8
    100 μl of BIS-TRIS propane pH 6.8 (1 M)
    325 μl of Polyethylene glycol 400 (100 v/v)
    60 μl of 1,4-Butanediol (100 v/v)
    150 μl of Sodium sulfate (1 M)
    365 μl of water (100 v/v)
    E9
    • Polyethylene glycol 400 32.5 v/v -
    • MES 0.1 M pH 6.2
    • Potassium Sodium tartrate 0.1 M -
    • Ethylene glycol 5 v/v -
    pH 6.2
    325 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Ethylene glycol (100 v/v)
    100 μl of Potassium Sodium tartrate (1 M)
    100 μl of MES pH 6.2 (1 M)
    425 μl of water (100 v/v)
    E10
    • Polyethylene glycol 400 34 v/v -
    • tri-Sodium citrate 0.09 M -
    • Ammonium sulfate 0.12 M -
    • tris chloride BCC 0.1 M pH 8.7
    pH 8.7
    340 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Ammonium sulfate (3 M)
    45 μl of tri-Sodium citrate (2 M)
    100 μl of tris chloride BCC pH 8.7 (1 M)
    475 μl of water (100 v/v)
    E11
    • Polyethylene glycol 400 34 v/v -
    • HEPES 0.1 M pH 7
    • Lithium sulfate 0.3 M -
    • Dimethyl sulfoxide 7.5 v/v -
    pH 7
    340 μl of Polyethylene glycol 400 (100 v/v)
    75 μl of Dimethyl sulfoxide (100 v/v)
    300 μl of Lithium sulfate (1 M)
    100 μl of HEPES pH 7 (1 M)
    185 μl of water (100 v/v)
    E12
    • Polyethylene glycol 400 35 v/v -
    • Glycerol 4 v/v -
    • tri-Sodium citrate 0.08 M -
    • TRICINE 0.1 M -
    NA
    350 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of TRICINE (1 M)
    40 μl of tri-Sodium citrate (2 M)
    40 μl of Glycerol (100 v/v)
    470 μl of water (100 v/v)
    F1
    • Polyethylene glycol 400 35 v/v -
    • tri-Sodium citrate 0.1 M pH 5
    • Ammonium acetate 0.37 M -
    • 1-Propanol 3 v/v -
    pH 5
    350 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of 1-Propanol (100 v/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    370 μl of Ammonium acetate (1 M)
    150 μl of water (100 v/v)
    F2
    • Polyethylene glycol 400 36 v/v -
    • Ammonium chloride 0.1 M -
    • HEPES 0.1 M pH 7.2
    pH 7.2
    360 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.2 (1 M)
    20 μl of Ammonium chloride (5 M)
    520 μl of water (100 v/v)
    F3
    • Polyethylene glycol 400 37.5 v/v -
    • di-Ammonium tartrate 0.18 M -
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • tri-Sodium citrate 0.1 M pH 6
    pH 6
    375 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Sodium citrate pH 6 (1 M)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    90 μl of di-Ammonium tartrate (2 M)
    395 μl of water (100 v/v)
    F4
    • Polyethylene glycol 400 38 v/v -
    • HEPES 0.1 M pH 7
    • Lithium chloride 0.3 M -
    • Strontium chloride 0.03 M -
    pH 7
    380 μl of Polyethylene glycol 400 (100 v/v)
    30 μl of Lithium chloride (10 M)
    300 μl of Strontium chloride (0.1 M)
    100 μl of HEPES pH 7 (1 M)
    190 μl of water (100 v/v)
    F5
    • Polyethylene glycol 400 39 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Potassium chloride 0.1 M -
    pH 8.5
    390 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Potassium chloride (1 M)
    410 μl of water (100 v/v)
    F6
    • Polyethylene glycol 400 39.8 v/v -
    • Ammonium di-hydrogen phosphate 0.1 M -
    • BIS-TRIS propane 0.1 M pH 7.2
    pH 7.2
    398 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Ammonium di-hydrogen phosphate (3 M)
    100 μl of BIS-TRIS propane pH 7.2 (1 M)
    468 μl of water (100 v/v)
    F7
    • Polyethylene glycol 400 40 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Dimethyl sulfoxide 4 v/v -
    • 1,7 Heptanediol 1 v/v -
    pH 8
    400 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of 1,7 Heptanediol (100 v/v)
    40 μl of Dimethyl sulfoxide (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    450 μl of water (100 v/v)
    F8
    • Polyethylene glycol 400 40 v/v -
    • MES 0.05 M pH 6.5
    • Sodium chloride 1.6 M -
    pH 6.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    320 μl of Sodium chloride (5 M)
    50 μl of MES pH 6.5 (1 M)
    230 μl of water (100 v/v)
    F9
    • Polyethylene glycol 400 40 v/v -
    • ADA 0.1 M pH 6.5
    • Lithium chloride 0.16 M -
    • Strontium chloride 0.004 M -
    pH 6.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    160 μl of Lithium chloride (1 M)
    100 μl of ADA pH 6.5 (1 M)
    40 μl of Strontium chloride (0.1 M)
    300 μl of water (100 v/v)
    F10
    • Polyethylene glycol 400 40 v/v -
    • ADA 0.1 M pH 6.5
    • Lithium sulfate 0.2 M -
    pH 6.5
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of ADA pH 6.5 (1 M)
    200 μl of Lithium sulfate (1 M)
    300 μl of water (100 v/v)
    F11
    • Polyethylene glycol 400 42 v/v -
    • MES 0.1 M pH 6.5
    • Sodium acetate 0.15 M -
    pH 6.5
    420 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    60 μl of Sodium acetate (2.5 M)
    420 μl of water (100 v/v)
    F12
    • MES 0.1 M pH 6
    • Copper (II) chloride 0.01 M -
    • Ammonium formate 0.2 M -
    • Polyethylene glycol dimethyl ether 500 25 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    100 μl of Copper (II) chloride (0.1 M)
    40 μl of Ammonium formate (5 M)
    250 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    510 μl of water (100 v/v)
    G1
    • MES 0.1 M pH 6
    • Cadmium chloride 0.002 M -
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    pH 6
    100 μl of MES pH 6 (1 M)
    20 μl of Cadmium chloride (0.1 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    580 μl of water (100 v/v)
    G2
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 6
    • Polyethylene glycol dimethyl ether 500 30 v/v -
    pH 6
    100 μl of Sodium chloride (1 M)
    40 μl of Magnesium chloride (2.5 M)
    33 μl of Ammonium sulfate (3 M)
    100 μl of tri-Sodium citrate pH 6 (1 M)
    300 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
    426 μl of water (100 v/v)
    G3
    • Polyethylene glycol monomethyl ether 550 14 v/v -
    • HEPES 0.1 M pH 7.5
    • Lithium sulfate 0.2 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    140 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    660 μl of water (100 v/v)
    G4
    • Polyethylene glycol monomethyl ether 550 15 v/v -
    • HEPES 0.1 M pH 7
    • Potassium Sodium tartrate 0.2 M -
    • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
    pH 7
    1 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
    200 μl of Potassium Sodium tartrate (1 M)
    100 μl of HEPES pH 7 (1 M)
    150 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    549 μl of water (100 v/v)
    G5
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    • Sodium nitrate 0.35 M -
    • di-Sodium malonate 0.05 M -
    • ADA 0.05 M pH 6.2
    pH 6.2
    16 μl of di-Sodium malonate pH 7 (3 M)
    50 μl of ADA pH 6.2 (1 M)
    50 μl of Sodium nitrate (7 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    633 μl of water (100 v/v)
    G6
    • Polyethylene glycol monomethyl ether 550 28 v/v -
    • tris chloride BCC 0.1 M pH 8
    • Lithium sulfate 0.1 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    280 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    570 μl of water (100 v/v)
    G7
    • Polyethylene glycol monomethyl ether 550 28 v/v -
    • ADA 0.05 M pH 7
    • Ammonium sulfate 0.55 M -
    pH 7
    183 μl of Ammonium sulfate (3 M)
    50 μl of ADA pH 7 (1 M)
    280 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    486 μl of water (100 v/v)
    G8
    • Polyethylene glycol 600 25 v/v -
    • ADA 0.1 M pH 7
    pH 7
    100 μl of ADA pH 7 (1 M)
    500 μl of Polyethylene glycol 600 (50 v/v)
    400 μl of water (100 v/v)
    G9
    • Polyethylene glycol 1,500 22.5 w/v -
    • MES 0.1 M pH 5.5
    • Sodium acetate 0.3 M -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    150 μl of Sodium acetate (2 M)
    450 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of water (100 v/v)
    G10
    • Polyethylene glycol 3,350 12 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • di-Sodium malonate 0.2 M -
    pH 4.6
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    66 μl of di-Sodium malonate (3 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    593 μl of water (100 v/v)
    G11
    • Polyethylene glycol 3,350 25 w/v -
    • BIS-TRIS 0.1 M pH 5.5
    • Lithium sulfate 0.2 M -
    pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    G12
    • Polyethylene glycol 3,350 25 w/v -
    • BIS-TRIS 0.1 M pH 5.5
    pH 5.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of water (100 v/v)
    H1
    • Polyethylene glycol 4,000 10 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Potassium chloride 0.2 M -
    pH 4.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Potassium chloride (1 M)
    100 μl of Sodium acetate pH 4.6 (1 M)
    500 μl of water (100 v/v)
    H2
    • Polyethylene glycol 6,000 20 w/v -
    • BICINE 0.1 M pH 9
    pH 9
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    500 μl of water (100 v/v)
    H3
    • Polyethylene glycol 8,000 30 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    66 μl of Ammonium sulfate (3 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of water (100 v/v)
    H4
    • Pentaerythritol Propoxylate (5/4 PO/OH) 10 w/v -
    • MES 0.1 M pH 6.5
    • Ammonium chloride 0.1 M -
    • Calcium chloride 0.01 M -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    166 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    10 μl of Calcium chloride (1 M)
    20 μl of Ammonium chloride (5 M)
    703 μl of water (100 v/v)
    H5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Potassium formate 0.1 M -
    pH 8
    333 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    7 μl of Potassium formate (14 M)
    559 μl of water (100 v/v)
    H6
    • 2-methyl-2,4-pentanediol BCC 4 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.1 M -
    • Lithium nitrate 0.1 M -
    pH 5.6
    100 μl of Sodium chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    12 μl of Lithium nitrate (8 M)
    40 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    747 μl of water (100 v/v)
    H7
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Sodium chloride 0.1 M -
    • Magnesium acetate 0.06 M -
    pH 5.6
    100 μl of Sodium chloride (1 M)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    60 μl of Magnesium acetate (1 M)
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    690 μl of water (100 v/v)
    H8
    • 2-methyl-2,4-pentanediol BCC 8 v/v -
    • Potassium nitrate 0.4 M -
    • tri-Potassium citrate 0.1 M -
    • ADA 0.1 M pH 6.7
    pH 6.7
    40 μl of tri-Potassium citrate (2.5 M)
    400 μl of Potassium nitrate (1 M)
    80 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of ADA pH 6.7 (1 M)
    380 μl of water (100 v/v)
    H9
    • D-(+)-Trehalose 0.3 M -
    • Sodium Potassium phosphate 1 M pH 5.1
    pH 5.1
    333 μl of Sodium Potassium phosphate pH 5.1 (3 M)
    300 μl of D-(+)-Trehalose (1 M)
    366 μl of water (100 v/v)
    H10
    • Sodium chloride 1.5 M -
    • Sodium acetate 0.075 M pH 4.6
    pH 4.6
    300 μl of Sodium chloride (5 M)
    75 μl of Sodium acetate pH 4.6 (1 M)
    625 μl of water (100 v/v)
    H11
    • Sodium acetate 1 M -
    • MES 0.1 M pH 6.5
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    500 μl of Sodium acetate (2 M)
    400 μl of water (100 v/v)
    H12
    • Lithium sulfate 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Nickel (II) chloride 0.01 M -
    pH 8.5
    10 μl of Nickel (II) chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Lithium sulfate (2.5 M)
    490 μl of water (100 v/v)