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Supplemental Information

Screen Description

LCP HTS

LCP / LCP HTS
Created 2016-09-29.

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Barcodes

LCP HTS

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Screen Structure

LCP HTS

Well Count 96
Number of distinct ingredients 75
Internal diversity (score mean dist) 0.884

Screen Composition

2025-08-16T04:27:17.499882 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type LCP HTS
Buffer 132
Cryoprotectant 1
Organic (non-volatile) 83
Organic (volatile) 5
Polymer 88
Salt 132

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
LCP HTS Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol 300 15 v/v -
  • HEPES 0.1 M pH 7.6
  • Ethylenediaminetetraacetic acid disodium salt 0.05 M -
  • 1,7 Heptanediol 1.2 v/v -
pH 7.6
12.0 μl of 1,7 Heptanediol (100 v/v)
100.0 μl of Ethylenediaminetetraacetic acid disodium salt pH 8 (0.5 M)
100.0 μl of HEPES pH 7.6 (1 M)
150.0 μl of Polyethylene glycol 300 (100 v/v)
638.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 300 30 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium di-hydrogen phosphate 0.1 M -
  • 2-methyl-2,4-pentanediol BCC 2 v/v -
pH 7.5
20.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
480.0 μl of water (100 v/v)
A3
  • Polyethylene glycol 300 30 v/v -
  • Sodium chloride 0.15 M -
  • Sodium di-hydrogen phosphate 0.1 M pH 6.3
pH 6.3
300.0 μl of Polyethylene glycol 300 (100 v/v)
150.0 μl of Sodium chloride (1 M)
100.0 μl of Sodium di-hydrogen phosphate pH 6.3 (1 M)
450.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 300 32 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium di-hydrogen phosphate 0.1 M -
  • 1,7 Heptanediol 1 v/v -
pH 7.5
10.0 μl of 1,7 Heptanediol (100 v/v)
100.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 300 (100 v/v)
470.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 300 37 v/v -
  • BIS-TRIS propane 0.1 M pH 6.5
  • di-Ammonium hydrogen phosphate 0.1 M -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
370.0 μl of Polyethylene glycol 300 (100 v/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
501.4 μl of water (100 v/v)
A6
  • Polyethylene glycol 400 10 v/v -
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.15 M -
pH 7.5
150.0 μl of Ammonium sulfate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
650.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 400 13 v/v -
  • MES 0.1 M pH 6
  • Potassium Sodium tartrate 0.17 M -
  • Jeffamine® M-600 0.45 v/v -
pH 6
9.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
100.0 μl of MES pH 6 (1 M)
130.0 μl of Polyethylene glycol 400 (100 v/v)
170.0 μl of Potassium Sodium tartrate (1 M)
591.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 400 14 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
pH 7.5
60.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
140.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
700.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 400 18 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • tri-Sodium citrate 0.1 M -
pH 7.5
180.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of tri-Sodium citrate (4 M)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
695.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 400 18 v/v -
  • HEPES 0.05 M pH 7
  • Potassium sulfate 0.1 M -
pH 7
50.0 μl of HEPES pH 7 (1 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
142.9 μl of Potassium sulfate (0.7 M)
627.1 μl of water (100 v/v)
A11
  • Polyethylene glycol 400 18.5 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Lithium sulfate 0.05 M -
  • Sodium chloride 0.1 M -
pH 5
16.7 μl of Lithium sulfate (3 M)
185.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
598.3 μl of water (100 v/v)
A12
  • Polyethylene glycol 400 19.5 v/v -
  • HEPES 0.1 M pH 7
  • Ammonium di-hydrogen phosphate 0.35 M -
pH 7
116.7 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of HEPES pH 7 (1 M)
195.0 μl of Polyethylene glycol 400 (100 v/v)
588.3 μl of water (100 v/v)
B1
  • Polyethylene glycol 400 20 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • di-Sodium malonate 0.3 M -
  • 2-Aminoethanesulfonic acid 0.005 M -
pH 5.5
50.0 μl of 2-Aminoethanesulfonic acid (0.1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Sodium malonate (3 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
550.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 400 20 v/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.4 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 400 20 v/v -
  • MES 0.1 M pH 6.5
  • Potassium nitrate 0.4 M -
  • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
133.3 μl of Potassium nitrate pH 6.9 (3 M)
1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
565.7 μl of water (100 v/v)
B4
  • Polyethylene glycol 400 21 v/v -
  • MES 0.1 M pH 6.5
  • di-Ammonium hydrogen phosphate 0.07 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
210.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of di-Ammonium hydrogen phosphate (3.5 M)
670.0 μl of water (100 v/v)
B5
  • Polyethylene glycol 400 23 v/v -
  • di-Sodium malonate 0.3 M -
  • Nickel (II) chloride 0.005 M -
  • tri-Sodium citrate 0.1 M pH 5.2
pH 5.2
5.0 μl of Nickel (II) chloride (1 M)
230.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of di-Sodium malonate (2 M)
100.0 μl of tri-Sodium citrate pH 5.2 (1 M)
515.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 400 25 v/v -
  • HEPES 0.1 M pH 7.5
  • Potassium Sodium tartrate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
200.0 μl of Potassium Sodium tartrate (1 M)
450.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 400 26 v/v -
  • Lithium sulfate 0.2 M -
  • 1,4-Butanediol 3.5 v/v -
  • Dimethyl sulfoxide 4 v/v -
  • MES 0.1 M pH 6.7
pH 6.7
35.0 μl of 1,4-Butanediol (100 v/v)
40.0 μl of Dimethyl sulfoxide (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of MES pH 6.7 (1 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
465.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 400 26 v/v -
  • MES 0.1 M pH 6
  • di-Sodium malonate 0.3 M -
  • Strontium chloride 0.005 M -
pH 6
100.0 μl of MES pH 6 (1 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Strontium chloride (0.1 M)
100.0 μl of di-Sodium malonate (3 M)
490.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 400 26 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Ammonium sulfate 0.3 M -
pH 8
100.0 μl of Ammonium sulfate (3 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
540.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 400 26.5 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Sodium thiocyanate 0.05 M -
  • 2,5-Hexanediol 2 v/v -
pH 5
50.0 μl of 2,5-Hexanediol (40 v/v)
265.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Sodium thiocyanate (2 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
560.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 400 27 v/v -
  • Sodium formate 0.22 M -
  • 1,4-Butanediol 5 v/v -
  • tris chloride BCC 0.1 M pH 7.8
pH 7.8
50.0 μl of 1,4-Butanediol (100 v/v)
270.0 μl of Polyethylene glycol 400 (100 v/v)
44.0 μl of Sodium formate (5 M)
100.0 μl of tris chloride BCC pH 7.8 (1 M)
536.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 400 27 v/v -
  • Ammonium fluoride 0.15 M -
  • Jeffamine® M-600 2.5 v/v -
  • HEPES 0.1 M pH 6.9
pH 6.9
15.0 μl of Ammonium fluoride (10 M)
100.0 μl of HEPES pH 6.9 (1 M)
50.0 μl of Jeffamine® M-600 (50 v/v)
270.0 μl of Polyethylene glycol 400 (100 v/v)
565.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 400 27.5 v/v -
  • di-Sodium tartrate 0.12 M -
  • 1,3-Butanediol 3 v/v -
  • BIS-TRIS propane 0.1 M pH 6.7
pH 6.7
75.0 μl of 1,3-Butanediol (40 v/v)
100.0 μl of BIS-TRIS propane pH 6.7 (1 M)
275.0 μl of Polyethylene glycol 400 (100 v/v)
60.0 μl of di-Sodium tartrate (2 M)
490.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 400 27.5 v/v -
  • Potassium Sodium tartrate 0.15 M -
  • BIS-TRIS propane 0.1 M pH 6.4
pH 6.4
100.0 μl of BIS-TRIS propane pH 6.4 (1 M)
275.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of Potassium Sodium tartrate (1 M)
475.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 400 28 v/v -
  • BIS-TRIS propane 0.1 M pH 7
  • Potassium formate 0.3 M -
pH 7
100.0 μl of BIS-TRIS propane pH 7 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
21.4 μl of Potassium formate (14 M)
598.6 μl of water (100 v/v)
C4
  • Polyethylene glycol 400 28 v/v -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Ammonium di-hydrogen phosphate 0.3 M -
  • Magnesium chloride 0.01 M -
pH 4.5
300.0 μl of Ammonium di-hydrogen phosphate (1 M)
4.0 μl of Magnesium chloride (2.5 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
316.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 400 28.5 v/v -
  • HEPES 0.1 M pH 7
  • di-Ammonium hydrogen phosphate 0.1 M -
  • Tris(2-carboxyethyl)phosphine hydrochloride 0.006 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
285.0 μl of Polyethylene glycol 400 (100 v/v)
6.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
580.4 μl of water (100 v/v)
C6
  • Polyethylene glycol 400 29 v/v -
  • di-Ammonium hydrogen phosphate 0.2 M -
  • MES 0.1 M pH 6.8
pH 6.8
100.0 μl of MES pH 6.8 (1 M)
290.0 μl of Polyethylene glycol 400 (100 v/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
552.9 μl of water (100 v/v)
C7
  • Polyethylene glycol 400 29 v/v -
  • tri-Sodium citrate 0.05 M pH 4
  • Lithium sulfate 0.2 M -
pH 4
100.0 μl of Lithium sulfate (2 M)
290.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tri-Sodium citrate pH 4 (1 M)
560.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 400 29.5 v/v -
  • Sodium formate 0.35 M -
  • 1,4-Butanediol 5 v/v -
  • tris chloride BCC 0.1 M pH 7.8
pH 7.8
50.0 μl of 1,4-Butanediol (100 v/v)
295.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 7.8 (1 M)
505.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium malonate 0.2 M -
pH 8
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Sodium malonate (2 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.1 M pH 6.5
  • Lithium sulfate 0.185 M -
pH 6.5
92.5 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 6.5 (1 M)
507.5 μl of water (100 v/v)
C11
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Magnesium chloride 0.2 M -
pH 5
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
520.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Lithium sulfate 0.2 M -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 400 30 v/v -
  • BIS-TRIS propane 0.1 M pH 7.5
  • Ammonium acetate 0.3 M -
  • D-(+)-Glucose 2 w/v -
pH 7.5
60.0 μl of Ammonium acetate (5 M)
100.0 μl of BIS-TRIS propane pH 7.5 (1 M)
66.7 μl of D-(+)-Glucose (30 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
473.3 μl of water (100 v/v)
D2
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
500.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6
  • Magnesium sulfate 0.1 M -
  • Polypropylene glycol 400 2.5 v/v -
pH 6
100.0 μl of MES pH 6 (1 M)
100.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Polypropylene glycol 400 (100 v/v)
475.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 400 30 v/v -
  • Potassium nitrate 0.4 M -
  • tri-Sodium citrate 0.1 M pH 6
pH 6
300.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of Potassium nitrate (1 M)
100.0 μl of tri-Sodium citrate pH 6 (1 M)
200.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.1 M pH 7.5
  • Lithium chloride 0.4 M -
pH 7.5
400.0 μl of Lithium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
200.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Magnesium sulfate 0.1 M -
pH 8
100.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 400 30 v/v -
  • Ammonium formate 0.1 M -
  • MES 0.1 M pH 6.3
pH 6.3
20.0 μl of Ammonium formate (5 M)
100.0 μl of MES pH 6.3 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
580.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7
  • Sodium thiocyanate 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium thiocyanate (2 M)
550.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 400 30 v/v -
  • MES 0.1 M pH 6.5
  • Sodium thiocyanate 0.1 M -
  • Calcium chloride 0.02 M -
pH 6.5
20.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium thiocyanate (2 M)
530.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 400 30 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Sodium thiocyanate 0.1 M -
  • Calcium chloride 0.02 M -
pH 8
20.0 μl of Calcium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium thiocyanate (2 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 400 30 v/v -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Sodium chloride 0.1 M -
  • D-(+)-Trehalose 3 w/v -
pH 5.5
100.0 μl of D-(+)-Trehalose (30 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 400 30 v/v -
  • MES 0.05 M pH 6.5
  • Magnesium chloride 0.1 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
610.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 400 30 v/v -
  • HEPES 0.1 M pH 7.5
  • ß-Mercaptoethanol 0.002 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
2.0 μl of ß-Mercaptoethanol (1 M)
598.0 μl of water (100 v/v)
E2
  • Polyethylene glycol 400 31 v/v -
  • MES 0.1 M pH 6
  • di-Sodium malonate 0.3 M -
  • Cobalt (III) Hexamine chloride 0.005 M -
pH 6
50.0 μl of Cobalt (III) Hexamine chloride (0.1 M)
100.0 μl of MES pH 6 (1 M)
310.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of di-Sodium malonate (3 M)
440.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 400 31 v/v -
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 0.15 M -
  • Magnesium chloride 0.35 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
140.0 μl of Magnesium chloride (2.5 M)
310.0 μl of Polyethylene glycol 400 (100 v/v)
75.0 μl of tri-Sodium citrate (2 M)
375.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 400 32 v/v -
  • HEPES 0.1 M pH 7.8
  • Ammonium fluoride 0.07 M -
  • Polypropylene glycol 400 6 v/v -
pH 7.8
7.0 μl of Ammonium fluoride (10 M)
100.0 μl of HEPES pH 7.8 (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
60.0 μl of Polypropylene glycol 400 (100 v/v)
513.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 400 32 v/v -
  • HEPES 0.1 M pH 7
  • Sodium chloride 0.1 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
480.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 32 v/v -
  • MES 0.05 M pH 6.5
  • Sodium thiocyanate 0.1 M -
  • Calcium chloride 0.02 M -
pH 6.5
20.0 μl of Calcium chloride (1 M)
50.0 μl of MES pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium thiocyanate (2 M)
560.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 400 32 v/v -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Sodium chloride 0.075 M -
  • Magnesium chloride 0.13 M -
pH 4.5
52.0 μl of Magnesium chloride (2.5 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
75.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
453.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 400 32.5 v/v -
  • Sodium sulfate 0.15 M -
  • 1,4-Butanediol 6 v/v -
  • BIS-TRIS propane 0.1 M pH 6.8
pH 6.8
60.0 μl of 1,4-Butanediol (100 v/v)
100.0 μl of BIS-TRIS propane pH 6.8 (1 M)
325.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of Sodium sulfate (1 M)
365.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 400 32.5 v/v -
  • MES 0.1 M pH 6.2
  • Potassium Sodium tartrate 0.1 M -
  • Ethylene glycol 5 v/v -
pH 6.2
50.0 μl of Ethylene glycol (100 v/v)
100.0 μl of MES pH 6.2 (1 M)
325.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium Sodium tartrate (1 M)
425.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 400 34 v/v -
  • tri-Sodium citrate 0.09 M -
  • Ammonium sulfate 0.12 M -
  • tris chloride BCC 0.1 M pH 8.7
pH 8.7
40.0 μl of Ammonium sulfate (3 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
45.0 μl of tri-Sodium citrate (2 M)
100.0 μl of tris chloride BCC pH 8.7 (1 M)
475.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 400 34 v/v -
  • HEPES 0.1 M pH 7
  • Lithium sulfate 0.3 M -
  • Dimethyl sulfoxide 7.5 v/v -
pH 7
75.0 μl of Dimethyl sulfoxide (100 v/v)
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Lithium sulfate (1 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
185.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 400 35 v/v -
  • Glycerol 4 v/v -
  • tri-Sodium citrate 0.08 M -
  • TRICINE 0.1 M -
NA
40.0 μl of Glycerol (100 v/v)
350.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of TRICINE (1 M)
40.0 μl of tri-Sodium citrate (2 M)
470.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 400 35 v/v -
  • tri-Sodium citrate 0.1 M pH 5
  • Ammonium acetate 0.37 M -
  • 1-Propanol 3 v/v -
pH 5
30.0 μl of 1-Propanol (100 v/v)
370.0 μl of Ammonium acetate (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
150.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 400 36 v/v -
  • Ammonium chloride 0.1 M -
  • HEPES 0.1 M pH 7.2
pH 7.2
20.0 μl of Ammonium chloride (5 M)
100.0 μl of HEPES pH 7.2 (1 M)
360.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 400 37.5 v/v -
  • di-Ammonium tartrate 0.18 M -
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • tri-Sodium citrate 0.1 M pH 6
pH 6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
375.0 μl of Polyethylene glycol 400 (100 v/v)
90.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of tri-Sodium citrate pH 6 (1 M)
395.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 400 38 v/v -
  • HEPES 0.1 M pH 7
  • Lithium chloride 0.3 M -
  • Strontium chloride 0.03 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
30.0 μl of Lithium chloride (10 M)
380.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of Strontium chloride (0.1 M)
190.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 400 39 v/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Potassium chloride 0.1 M -
pH 8.5
390.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
410.0 μl of water (100 v/v)
F6
  • Polyethylene glycol 400 39.8 v/v -
  • Ammonium di-hydrogen phosphate 0.1 M -
  • BIS-TRIS propane 0.1 M pH 7.2
pH 7.2
33.3 μl of Ammonium di-hydrogen phosphate (3 M)
100.0 μl of BIS-TRIS propane pH 7.2 (1 M)
398.0 μl of Polyethylene glycol 400 (100 v/v)
468.7 μl of water (100 v/v)
F7
  • Polyethylene glycol 400 40 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Dimethyl sulfoxide 4 v/v -
  • 1,7 Heptanediol 1 v/v -
pH 8
10.0 μl of 1,7 Heptanediol (100 v/v)
40.0 μl of Dimethyl sulfoxide (100 v/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
450.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 400 40 v/v -
  • MES 0.05 M pH 6.5
  • Sodium chloride 1.6 M -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
320.0 μl of Sodium chloride (5 M)
230.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 400 40 v/v -
  • ADA 0.1 M pH 6.5
  • Lithium chloride 0.16 M -
  • Strontium chloride 0.004 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
160.0 μl of Lithium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Strontium chloride (0.1 M)
300.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 400 40 v/v -
  • ADA 0.1 M pH 6.5
  • Lithium sulfate 0.2 M -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
200.0 μl of Lithium sulfate (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 400 42 v/v -
  • MES 0.1 M pH 6.5
  • Sodium acetate 0.15 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
420.0 μl of Polyethylene glycol 400 (100 v/v)
60.0 μl of Sodium acetate (2.5 M)
420.0 μl of water (100 v/v)
F12
  • MES 0.1 M pH 6
  • Copper (II) chloride 0.01 M -
  • Ammonium formate 0.2 M -
  • Polyethylene glycol dimethyl ether 500 25 v/v -
pH 6
40.0 μl of Ammonium formate (5 M)
100.0 μl of Copper (II) chloride (0.1 M)
100.0 μl of MES pH 6 (1 M)
250.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
510.0 μl of water (100 v/v)
G1
  • MES 0.1 M pH 6
  • Cadmium chloride 0.002 M -
  • Polyethylene glycol dimethyl ether 500 30 v/v -
pH 6
20.0 μl of Cadmium chloride (0.1 M)
100.0 μl of MES pH 6 (1 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
580.0 μl of water (100 v/v)
G2
  • Magnesium chloride 0.1 M -
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
pH 6
33.3 μl of Ammonium sulfate (3 M)
40.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 6 (1 M)
426.7 μl of water (100 v/v)
G3
  • Polyethylene glycol monomethyl ether 550 14 v/v -
  • HEPES 0.1 M pH 7.5
  • Lithium sulfate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
140.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
660.0 μl of water (100 v/v)
G4
  • Polyethylene glycol monomethyl ether 550 15 v/v -
  • HEPES 0.1 M pH 7
  • Potassium Sodium tartrate 0.2 M -
  • Tris(2-carboxyethyl)phosphine hydrochloride 0.001 M -
pH 7
100.0 μl of HEPES pH 7 (1 M)
150.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
200.0 μl of Potassium Sodium tartrate (1 M)
1.0 μl of Tris(2-carboxyethyl)phosphine hydrochloride (1 M)
549.0 μl of water (100 v/v)
G5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • Sodium nitrate 0.35 M -
  • di-Sodium malonate 0.05 M -
  • ADA 0.05 M pH 6.2
pH 6.2
50.0 μl of ADA pH 6.2 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Sodium nitrate (7 M)
16.7 μl of di-Sodium malonate pH 7 (3 M)
633.3 μl of water (100 v/v)
G6
  • Polyethylene glycol monomethyl ether 550 28 v/v -
  • tris chloride BCC 0.1 M pH 8
  • Lithium sulfate 0.1 M -
pH 8
50.0 μl of Lithium sulfate (2 M)
280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
570.0 μl of water (100 v/v)
G7
  • Polyethylene glycol monomethyl ether 550 28 v/v -
  • ADA 0.05 M pH 7
  • Ammonium sulfate 0.55 M -
pH 7
50.0 μl of ADA pH 7 (1 M)
183.3 μl of Ammonium sulfate (3 M)
280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
486.7 μl of water (100 v/v)
G8
  • Polyethylene glycol 600 25 v/v -
  • ADA 0.1 M pH 7
pH 7
100.0 μl of ADA pH 7 (1 M)
500.0 μl of Polyethylene glycol 600 (50 v/v)
400.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 1,500 22.5 w/v -
  • MES 0.1 M pH 5.5
  • Sodium acetate 0.3 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
450.0 μl of Polyethylene glycol 1,500 (50 w/v)
150.0 μl of Sodium acetate (2 M)
300.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 3,350 12 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • di-Sodium malonate 0.2 M -
pH 4.6
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
66.7 μl of di-Sodium malonate (3 M)
593.3 μl of water (100 v/v)
G11
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Lithium sulfate 0.2 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS 0.1 M pH 5.5
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 4,000 10 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Potassium chloride 0.2 M -
pH 4.6
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Potassium chloride (1 M)
100.0 μl of Sodium acetate pH 4.6 (1 M)
500.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 9
pH 9
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 8,000 30 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
333.3 μl of water (100 v/v)
H4
  • Pentaerythritol Propoxylate (5/4 PO/OH) 10 w/v -
  • MES 0.1 M pH 6.5
  • Ammonium chloride 0.1 M -
  • Calcium chloride 0.01 M -
pH 6.5
20.0 μl of Ammonium chloride (5 M)
10.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
166.7 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
703.3 μl of water (100 v/v)
H5
  • Pentaerythritol Propoxylate (5/4 PO/OH) 20 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Potassium formate 0.1 M -
pH 8
333.3 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (60 w/v)
7.1 μl of Potassium formate (14 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
559.5 μl of water (100 v/v)
H6
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.1 M -
  • Lithium nitrate 0.1 M -
pH 5.6
40.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
12.5 μl of Lithium nitrate (8 M)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
747.5 μl of water (100 v/v)
H7
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Sodium chloride 0.1 M -
  • Magnesium acetate 0.06 M -
pH 5.6
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
60.0 μl of Magnesium acetate (1 M)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
690.0 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 8 v/v -
  • Potassium nitrate 0.4 M -
  • tri-Potassium citrate 0.1 M -
  • ADA 0.1 M pH 6.7
pH 6.7
80.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of ADA pH 6.7 (1 M)
400.0 μl of Potassium nitrate (1 M)
40.0 μl of tri-Potassium citrate (2.5 M)
380.0 μl of water (100 v/v)
H9
  • D-(+)-Trehalose 0.3 M -
  • Sodium Potassium phosphate 1 M pH 5.1
pH 5.1
300.0 μl of D-(+)-Trehalose (1 M)
333.3 μl of Sodium Potassium phosphate pH 5.1 (3 M)
366.7 μl of water (100 v/v)
H10
  • Sodium chloride 1.5 M -
  • Sodium acetate 0.075 M pH 4.6
pH 4.6
75.0 μl of Sodium acetate pH 4.6 (1 M)
300.0 μl of Sodium chloride (5 M)
625.0 μl of water (100 v/v)
H11
  • Sodium acetate 1 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Sodium acetate (2 M)
400.0 μl of water (100 v/v)
H12
  • Lithium sulfate 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Nickel (II) chloride 0.01 M -
pH 8.5
400.0 μl of Lithium sulfate (2.5 M)
10.0 μl of Nickel (II) chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)