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Supplemental Information

Screen Description

GRAS5

GRAS screens / GRAS5
Created 2016-09-29.

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GRAS5

    Screen Structure

    GRAS5

    Well Count 96
    Number of distinct ingredients 12
    Internal diversity (score mean dist) 0.895

    Screen Composition

    2025-06-15T02:22:18.378065 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type GRAS5
    Buffer 96
    Organic (non-volatile) 48
    Polymer 96
    Salt 36

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    GRAS5 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 8 v/v -
    pH 4.5
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 26 v/v -
    pH 4.5
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    640 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 44 v/v -
    pH 4.5
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    460 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 8 v/v -
    pH 4.5
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    A5
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 26 v/v -
    pH 4.5
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    640 μl of water (100 v/v)
    A6
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 44 v/v -
    pH 4.5
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    460 μl of water (100 v/v)
    A7
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 4.5
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 4.5
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    640 μl of water (100 v/v)
    A9
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 4.5
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    460 μl of water (100 v/v)
    A10
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 600 8 v/v -
    pH 4.5
    80 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    A11
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 600 26 v/v -
    pH 4.5
    260 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    640 μl of water (100 v/v)
    A12
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 600 44 v/v -
    pH 4.5
    440 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    460 μl of water (100 v/v)
    B1
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 300 8 v/v -
    pH 5.5
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    820 μl of water (100 v/v)
    B2
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 300 26 v/v -
    pH 5.5
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    640 μl of water (100 v/v)
    B3
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 300 44 v/v -
    pH 5.5
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    460 μl of water (100 v/v)
    B4
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 400 8 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol 400 (100 v/v)
    820 μl of water (100 v/v)
    B5
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 400 26 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    640 μl of water (100 v/v)
    B6
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 400 44 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    440 μl of Polyethylene glycol 400 (100 v/v)
    460 μl of water (100 v/v)
    B7
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    820 μl of water (100 v/v)
    B8
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    640 μl of water (100 v/v)
    B9
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    460 μl of water (100 v/v)
    B10
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 600 8 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    80 μl of Polyethylene glycol 600 (100 v/v)
    820 μl of water (100 v/v)
    B11
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 600 26 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    640 μl of water (100 v/v)
    B12
    • di-Sodium succinate 0.1 M pH 5.5
    • Polyethylene glycol 600 44 v/v -
    pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    440 μl of Polyethylene glycol 600 (100 v/v)
    460 μl of water (100 v/v)
    C1
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 300 8 v/v -
    pH 6.5
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    820 μl of water (100 v/v)
    C2
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 300 26 v/v -
    pH 6.5
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    640 μl of water (100 v/v)
    C3
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 300 44 v/v -
    pH 6.5
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of water (100 v/v)
    C4
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 8 v/v -
    pH 6.5
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    820 μl of water (100 v/v)
    C5
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 26 v/v -
    pH 6.5
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    640 μl of water (100 v/v)
    C6
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 400 44 v/v -
    pH 6.5
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of water (100 v/v)
    C7
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 6.5
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    820 μl of water (100 v/v)
    C8
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 6.5
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    640 μl of water (100 v/v)
    C9
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 6.5
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of water (100 v/v)
    C10
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 600 8 v/v -
    pH 6.5
    80 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    820 μl of water (100 v/v)
    C11
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 600 26 v/v -
    pH 6.5
    260 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    640 μl of water (100 v/v)
    C12
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 600 44 v/v -
    pH 6.5
    440 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    460 μl of water (100 v/v)
    D1
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 300 8 v/v -
    pH 7
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    820 μl of water (100 v/v)
    D2
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 300 26 v/v -
    pH 7
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    640 μl of water (100 v/v)
    D3
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 300 44 v/v -
    pH 7
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    460 μl of water (100 v/v)
    D4
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 400 8 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol 400 (100 v/v)
    820 μl of water (100 v/v)
    D5
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 400 26 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    640 μl of water (100 v/v)
    D6
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 400 44 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    440 μl of Polyethylene glycol 400 (100 v/v)
    460 μl of water (100 v/v)
    D7
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    820 μl of water (100 v/v)
    D8
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    640 μl of water (100 v/v)
    D9
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    460 μl of water (100 v/v)
    D10
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 600 8 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    80 μl of Polyethylene glycol 600 (100 v/v)
    820 μl of water (100 v/v)
    D11
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 600 26 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    640 μl of water (100 v/v)
    D12
    • Sodium Potassium phosphate 0.1 M pH 7
    • Polyethylene glycol 600 44 v/v -
    pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    440 μl of Polyethylene glycol 600 (100 v/v)
    460 μl of water (100 v/v)
    E1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 300 8 v/v -
    pH 7.5
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    E2
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 300 26 v/v -
    pH 7.5
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    640 μl of water (100 v/v)
    E3
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 300 44 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    E4
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 8 v/v -
    pH 7.5
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 26 v/v -
    pH 7.5
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    640 μl of water (100 v/v)
    E6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 44 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    E7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 7.5
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    E8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 7.5
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    640 μl of water (100 v/v)
    E9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 7.5
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 600 8 v/v -
    pH 7.5
    80 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    E11
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 600 26 v/v -
    pH 7.5
    260 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    640 μl of water (100 v/v)
    E12
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 600 44 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    460 μl of water (100 v/v)
    F1
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 8 v/v -
    pH 8
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    820 μl of water (100 v/v)
    F2
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 26 v/v -
    pH 8
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    640 μl of water (100 v/v)
    F3
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 44 v/v -
    pH 8
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    460 μl of water (100 v/v)
    F4
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 8 v/v -
    pH 8
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    820 μl of water (100 v/v)
    F5
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 26 v/v -
    pH 8
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    640 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 44 v/v -
    pH 8
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    460 μl of water (100 v/v)
    F7
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    820 μl of water (100 v/v)
    F8
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    640 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    460 μl of water (100 v/v)
    F10
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 8 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    80 μl of Polyethylene glycol 600 (100 v/v)
    820 μl of water (100 v/v)
    F11
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 26 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    640 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 600 44 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    440 μl of Polyethylene glycol 600 (100 v/v)
    460 μl of water (100 v/v)
    G1
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 300 8 v/v -
    pH 9
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    820 μl of water (100 v/v)
    G2
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 300 26 v/v -
    pH 9
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    640 μl of water (100 v/v)
    G3
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 300 44 v/v -
    pH 9
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    460 μl of water (100 v/v)
    G4
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 400 8 v/v -
    pH 9
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    820 μl of water (100 v/v)
    G5
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 400 26 v/v -
    pH 9
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    640 μl of water (100 v/v)
    G6
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 400 44 v/v -
    pH 9
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    460 μl of water (100 v/v)
    G7
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 9
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    820 μl of water (100 v/v)
    G8
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 9
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    640 μl of water (100 v/v)
    G9
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 9
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    460 μl of water (100 v/v)
    G10
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 600 8 v/v -
    pH 9
    80 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    820 μl of water (100 v/v)
    G11
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 600 26 v/v -
    pH 9
    260 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    640 μl of water (100 v/v)
    G12
    • BIS-TRIS propane 0.1 M pH 9
    • Polyethylene glycol 600 44 v/v -
    pH 9
    440 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    460 μl of water (100 v/v)
    H1
    • CHES 0.1 M pH 10
    • Polyethylene glycol 300 8 v/v -
    pH 10
    80 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    820 μl of water (100 v/v)
    H2
    • CHES 0.1 M pH 10
    • Polyethylene glycol 300 26 v/v -
    pH 10
    260 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    640 μl of water (100 v/v)
    H3
    • CHES 0.1 M pH 10
    • Polyethylene glycol 300 44 v/v -
    pH 10
    440 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    460 μl of water (100 v/v)
    H4
    • CHES 0.1 M pH 10
    • Polyethylene glycol 400 8 v/v -
    pH 10
    80 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    820 μl of water (100 v/v)
    H5
    • CHES 0.1 M pH 10
    • Polyethylene glycol 400 26 v/v -
    pH 10
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    640 μl of water (100 v/v)
    H6
    • CHES 0.1 M pH 10
    • Polyethylene glycol 400 44 v/v -
    pH 10
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    460 μl of water (100 v/v)
    H7
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 550 8 v/v -
    pH 10
    100 μl of CHES pH 10 (1 M)
    80 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    820 μl of water (100 v/v)
    H8
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 10
    100 μl of CHES pH 10 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    640 μl of water (100 v/v)
    H9
    • CHES 0.1 M pH 10
    • Polyethylene glycol monomethyl ether 550 44 v/v -
    pH 10
    100 μl of CHES pH 10 (1 M)
    440 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    460 μl of water (100 v/v)
    H10
    • CHES 0.1 M pH 10
    • Polyethylene glycol 600 8 v/v -
    pH 10
    80 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    820 μl of water (100 v/v)
    H11
    • CHES 0.1 M pH 10
    • Polyethylene glycol 600 26 v/v -
    pH 10
    260 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    640 μl of water (100 v/v)
    H12
    • CHES 0.1 M pH 10
    • Polyethylene glycol 600 44 v/v -
    pH 10
    440 μl of Polyethylene glycol 600 (100 v/v)
    100 μl of CHES pH 10 (1 M)
    460 μl of water (100 v/v)