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Supplemental Information

Screen Description

GRAS7

GRAS screens / GRAS7
Created 2016-09-29.

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GRAS7

Barcodes

GRAS7

    Screen Structure

    GRAS7

    Well Count 96
    Number of distinct ingredients 18
    Internal diversity (score mean dist) 0.912

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:47:23.424824 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type GRAS7
    Buffer 152
    Salt 133

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    GRAS7 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    900 μl of water (100 v/v)
    A2
    • di-Sodium succinate 0.1 M pH 5.5
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    900 μl of water (100 v/v)
    A3
    • BIS-TRIS 0.1 M pH 6.5
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    900 μl of water (100 v/v)
    A4
    • Sodium Potassium phosphate 0.1 M pH 7
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    900 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 7.5
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    900 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    900 μl of water (100 v/v)
    A7
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    900 μl of water (100 v/v)
    A8
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    900 μl of water (100 v/v)
    A9
    • tris chloride BCC 0.1 M pH 8
    • Ammonium acetate 0.2 M -
    		pH 8
    25 μl of Ammonium acetate (8 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    875 μl of water (100 v/v)
    A10
    • tris chloride BCC 0.1 M pH 8
    • Ammonium acetate 1.5 M -
    		pH 8
    187 μl of Ammonium acetate (8 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    712 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.1 M pH 8
    • Ammonium acetate 2.7 M -
    		pH 8
    337 μl of Ammonium acetate (8 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    562 μl of water (100 v/v)
    A12
    • tris chloride BCC 0.1 M pH 8
    • Ammonium acetate 4 M -
    		pH 8
    500 μl of Ammonium acetate (8 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of water (100 v/v)
    B1
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium chloride 0.2 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    40 μl of Ammonium chloride (5 M)
    860 μl of water (100 v/v)
    B2
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium chloride 1.3 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    260 μl of Ammonium chloride (5 M)
    640 μl of water (100 v/v)
    B3
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium chloride 2.4 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    480 μl of Ammonium chloride (5 M)
    420 μl of water (100 v/v)
    B4
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium chloride 3.5 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    700 μl of Ammonium chloride (5 M)
    200 μl of water (100 v/v)
    B5
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium chloride 0.2 M -
    		pH 5.5
    40 μl of Ammonium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    860 μl of water (100 v/v)
    B6
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium chloride 1.3 M -
    		pH 5.5
    260 μl of Ammonium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    640 μl of water (100 v/v)
    B7
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium chloride 2.4 M -
    		pH 5.5
    480 μl of Ammonium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    420 μl of water (100 v/v)
    B8
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium chloride 3.5 M -
    		pH 5.5
    700 μl of Ammonium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    200 μl of water (100 v/v)
    B9
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium chloride 0.2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    40 μl of Ammonium chloride (5 M)
    860 μl of water (100 v/v)
    B10
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium chloride 1.3 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    260 μl of Ammonium chloride (5 M)
    640 μl of water (100 v/v)
    B11
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium chloride 2.4 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    480 μl of Ammonium chloride (5 M)
    420 μl of water (100 v/v)
    B12
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium chloride 3.5 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    700 μl of Ammonium chloride (5 M)
    200 μl of water (100 v/v)
    C1
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 0.2 M -
    		pH 4.5
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    C2
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 0.7 M -
    		pH 4.5
    280 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    620 μl of water (100 v/v)
    C3
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 1.3 M -
    		pH 4.5
    520 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    380 μl of water (100 v/v)
    C4
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 1.8 M -
    		pH 4.5
    720 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    180 μl of water (100 v/v)
    C5
    • HEPES 0.1 M pH 7.5
    • di-Ammonium hydrogen citrate 0.2 M -
    		pH 7.5
    80 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    820 μl of water (100 v/v)
    C6
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 0.7 M -
    		pH 4.5
    280 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    620 μl of water (100 v/v)
    C7
    • Sodium acetate 0.1 M pH 4.5
    • di-Ammonium hydrogen citrate 1.3 M -
    		pH 4.5
    520 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    380 μl of water (100 v/v)
    C8
    • HEPES 0.1 M pH 7.5
    • di-Ammonium hydrogen citrate 1.8 M -
    		pH 7.5
    720 μl of di-Ammonium hydrogen citrate (2.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    180 μl of water (100 v/v)
    C9
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium formate 0.2 M -
    		pH 7
    20 μl of Ammonium formate (10 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    880 μl of water (100 v/v)
    C10
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium formate 1.1 M -
    		pH 7
    110 μl of Ammonium formate (10 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    790 μl of water (100 v/v)
    C11
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium formate 2 M -
    		pH 7
    200 μl of Ammonium formate (10 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    700 μl of water (100 v/v)
    C12
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium formate 3 M -
    		pH 7
    300 μl of Ammonium formate (10 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    600 μl of water (100 v/v)
    D1
    • tris chloride BCC 0.1 M pH 8
    • di-Ammonium hydrogen phosphate 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    842 μl of water (100 v/v)
    D2
    • tris chloride BCC 0.1 M pH 8
    • di-Ammonium hydrogen phosphate 1 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    285 μl of di-Ammonium hydrogen phosphate (3.5 M)
    614 μl of water (100 v/v)
    D3
    • tris chloride BCC 0.1 M pH 8
    • di-Ammonium hydrogen phosphate 1.7 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    485 μl of di-Ammonium hydrogen phosphate (3.5 M)
    414 μl of water (100 v/v)
    D4
    • tris chloride BCC 0.1 M pH 8
    • di-Ammonium hydrogen phosphate 2.5 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    714 μl of di-Ammonium hydrogen phosphate (3.5 M)
    185 μl of water (100 v/v)
    D5
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium di-hydrogen phosphate 0.2 M -
    		pH 4.5
    80 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    820 μl of water (100 v/v)
    D6
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium di-hydrogen phosphate 0.8 M -
    		pH 4.5
    320 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    580 μl of water (100 v/v)
    D7
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium di-hydrogen phosphate 1.4 M -
    		pH 4.5
    560 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    340 μl of water (100 v/v)
    D8
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium di-hydrogen phosphate 2 M -
    		pH 4.5
    800 μl of Ammonium di-hydrogen phosphate (2.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    100 μl of water (100 v/v)
    D9
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 0.2 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    842 μl of water (100 v/v)
    D10
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 0.8 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    D11
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 1.4 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of Ammonium sulfate (3.5 M)
    500 μl of water (100 v/v)
    D12
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 2 M -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    E1
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium sulfate 0.2 M -
    		pH 5.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    842 μl of water (100 v/v)
    E2
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium sulfate 0.8 M -
    		pH 5.5
    228 μl of Ammonium sulfate (3.5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    671 μl of water (100 v/v)
    E3
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium sulfate 1.4 M -
    		pH 5.5
    400 μl of Ammonium sulfate (3.5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    E4
    • di-Sodium succinate 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    		pH 5.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    328 μl of water (100 v/v)
    E5
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 0.2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    842 μl of water (100 v/v)
    E6
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 0.8 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    228 μl of Ammonium sulfate (3.5 M)
    671 μl of water (100 v/v)
    E7
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 1.4 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Ammonium sulfate (3.5 M)
    500 μl of water (100 v/v)
    E8
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    E9
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium sulfate 0.2 M -
    		pH 7
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    842 μl of water (100 v/v)
    E10
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium sulfate 0.8 M -
    		pH 7
    228 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    671 μl of water (100 v/v)
    E11
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium sulfate 1.4 M -
    		pH 7
    400 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    500 μl of water (100 v/v)
    E12
    • Sodium Potassium phosphate 0.1 M pH 7
    • Ammonium sulfate 2 M -
    		pH 7
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    328 μl of water (100 v/v)
    F1
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.2 M -
    		pH 7.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    842 μl of water (100 v/v)
    F2
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 0.8 M -
    		pH 7.5
    228 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    671 μl of water (100 v/v)
    F3
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 1.4 M -
    		pH 7.5
    400 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    F4
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    		pH 7.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    328 μl of water (100 v/v)
    F5
    • di-Sodium succinate 0.1 M pH 5.5
    • di-Ammonium tartrate 0.2 M -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    100 μl of di-Ammonium tartrate (2 M)
    800 μl of water (100 v/v)
    F6
    • di-Sodium succinate 0.1 M pH 5.5
    • di-Ammonium tartrate 0.6 M -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    300 μl of di-Ammonium tartrate (2 M)
    600 μl of water (100 v/v)
    F7
    • di-Sodium succinate 0.1 M pH 5.5
    • di-Ammonium tartrate 1 M -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    500 μl of di-Ammonium tartrate (2 M)
    400 μl of water (100 v/v)
    F8
    • di-Sodium succinate 0.1 M pH 5.5
    • di-Ammonium tartrate 1.5 M -
    		pH 5.5
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    750 μl of di-Ammonium tartrate (2 M)
    150 μl of water (100 v/v)
    F9
    • BIS-TRIS 0.1 M pH 6.5
    • di-Ammonium tartrate 0.2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of di-Ammonium tartrate (2 M)
    800 μl of water (100 v/v)
    F10
    • BIS-TRIS 0.1 M pH 6.5
    • di-Ammonium tartrate 0.6 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    300 μl of di-Ammonium tartrate (2 M)
    600 μl of water (100 v/v)
    F11
    • BIS-TRIS 0.1 M pH 6.5
    • di-Ammonium tartrate 1 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of di-Ammonium tartrate (2 M)
    400 μl of water (100 v/v)
    F12
    • BIS-TRIS 0.1 M pH 6.5
    • di-Ammonium tartrate 1.5 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    750 μl of di-Ammonium tartrate (2 M)
    150 μl of water (100 v/v)
    G1
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Ammonium tartrate 0.2 M -
    		pH 7
    100 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    800 μl of water (100 v/v)
    G2
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Ammonium tartrate 0.6 M -
    		pH 7
    300 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    600 μl of water (100 v/v)
    G3
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Ammonium tartrate 1 M -
    		pH 7
    500 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    400 μl of water (100 v/v)
    G4
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Ammonium tartrate 1.5 M -
    		pH 7
    750 μl of di-Ammonium tartrate (2 M)
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    150 μl of water (100 v/v)
    G5
    • HEPES 0.1 M pH 7.5
    • di-Ammonium tartrate 0.2 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of di-Ammonium tartrate (2 M)
    800 μl of water (100 v/v)
    G6
    • HEPES 0.1 M pH 7.5
    • di-Ammonium tartrate 0.6 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of di-Ammonium tartrate (2 M)
    600 μl of water (100 v/v)
    G7
    • HEPES 0.1 M pH 7.5
    • di-Ammonium tartrate 1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of di-Ammonium tartrate (2 M)
    400 μl of water (100 v/v)
    G8
    • HEPES 0.1 M pH 7.5
    • di-Ammonium tartrate 1.5 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    750 μl of di-Ammonium tartrate (2 M)
    150 μl of water (100 v/v)
    G9
    • CHES 0.1 M pH 10
    • di-Potassium hydrogen phosphate 0.2 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    66 μl of di-Potassium hydrogen phosphate (3 M)
    833 μl of water (100 v/v)
    G10
    • CHES 0.1 M pH 10
    • di-Potassium hydrogen phosphate 0.7 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    233 μl of di-Potassium hydrogen phosphate (3 M)
    666 μl of water (100 v/v)
    G11
    • CHES 0.1 M pH 10
    • di-Potassium hydrogen phosphate 1.3 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    433 μl of di-Potassium hydrogen phosphate (3 M)
    466 μl of water (100 v/v)
    G12
    • CHES 0.1 M pH 10
    • di-Potassium hydrogen phosphate 1.8 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    600 μl of di-Potassium hydrogen phosphate (3 M)
    300 μl of water (100 v/v)
    H1
    • tris chloride BCC 0.1 M pH 8
    • Sodium acetate 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    66 μl of Sodium acetate (3 M)
    833 μl of water (100 v/v)
    H2
    • tris chloride BCC 0.1 M pH 8
    • Sodium acetate 0.7 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    233 μl of Sodium acetate (3 M)
    666 μl of water (100 v/v)
    H3
    • tris chloride BCC 0.1 M pH 8
    • Sodium acetate 1.3 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    433 μl of Sodium acetate (3 M)
    466 μl of water (100 v/v)
    H4
    • tris chloride BCC 0.1 M pH 8
    • Sodium acetate 1.8 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    600 μl of Sodium acetate (3 M)
    300 μl of water (100 v/v)
    H5
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium acetate 0.2 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    66 μl of Sodium acetate (3 M)
    833 μl of water (100 v/v)
    H6
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium acetate 0.7 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    233 μl of Sodium acetate (3 M)
    666 μl of water (100 v/v)
    H7
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium acetate 1.3 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    433 μl of Sodium acetate (3 M)
    466 μl of water (100 v/v)
    H8
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium acetate 1.8 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    600 μl of Sodium acetate (3 M)
    300 μl of water (100 v/v)
    H9
    • CHES 0.1 M pH 10
    • Sodium acetate 0.2 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    66 μl of Sodium acetate (3 M)
    833 μl of water (100 v/v)
    H10
    • CHES 0.1 M pH 10
    • Sodium acetate 0.7 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    233 μl of Sodium acetate (3 M)
    666 μl of water (100 v/v)
    H11
    • CHES 0.1 M pH 10
    • Sodium acetate 1.3 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    433 μl of Sodium acetate (3 M)
    466 μl of water (100 v/v)
    H12
    • CHES 0.1 M pH 10
    • Sodium acetate 1.8 M -
    		pH 10
    100 μl of CHES pH 10 (1 M)
    600 μl of Sodium acetate (3 M)
    300 μl of water (100 v/v)