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Supplemental Information

Screen Description

GRAS7

GRAS screens / GRAS7
Created 2016-09-29.

Export

GRAS7

Barcodes

GRAS7

No barcodes found.

Screen Structure

GRAS7

Well Count 96
Number of distinct ingredients 18
Internal diversity (score mean dist) 0.912

Screen Composition

More information about screen composition chart.
2025-08-12T11:04:36.866093 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type GRAS7
Buffer 152
Salt 133

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
GRAS7 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
900.0 μl of water (100 v/v)
A2
  • di-Sodium succinate 0.1 M pH 5.5
pH 5.5
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
900.0 μl of water (100 v/v)
A3
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
900.0 μl of water (100 v/v)
A4
  • Sodium Potassium phosphate 0.1 M pH 7
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
900.0 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
900.0 μl of water (100 v/v)
A6
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of tris chloride BCC pH 8 (1 M)
900.0 μl of water (100 v/v)
A7
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
900.0 μl of water (100 v/v)
A8
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
900.0 μl of water (100 v/v)
A9
  • tris chloride BCC 0.1 M pH 8
  • Ammonium acetate 0.2 M -
pH 8
25.0 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
875.0 μl of water (100 v/v)
A10
  • tris chloride BCC 0.1 M pH 8
  • Ammonium acetate 1.5 M -
pH 8
187.5 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
712.5 μl of water (100 v/v)
A11
  • tris chloride BCC 0.1 M pH 8
  • Ammonium acetate 2.7 M -
pH 8
337.5 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
562.5 μl of water (100 v/v)
A12
  • tris chloride BCC 0.1 M pH 8
  • Ammonium acetate 4 M -
pH 8
500.0 μl of Ammonium acetate (8 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
B1
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium chloride 0.2 M -
pH 4.5
40.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
860.0 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium chloride 1.3 M -
pH 4.5
260.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
640.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium chloride 2.4 M -
pH 4.5
480.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
420.0 μl of water (100 v/v)
B4
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium chloride 3.5 M -
pH 4.5
700.0 μl of Ammonium chloride (5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
200.0 μl of water (100 v/v)
B5
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium chloride 0.2 M -
pH 5.5
40.0 μl of Ammonium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
860.0 μl of water (100 v/v)
B6
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium chloride 1.3 M -
pH 5.5
260.0 μl of Ammonium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
640.0 μl of water (100 v/v)
B7
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium chloride 2.4 M -
pH 5.5
480.0 μl of Ammonium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
420.0 μl of water (100 v/v)
B8
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium chloride 3.5 M -
pH 5.5
700.0 μl of Ammonium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
200.0 μl of water (100 v/v)
B9
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium chloride 0.2 M -
pH 6.5
40.0 μl of Ammonium chloride (5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
860.0 μl of water (100 v/v)
B10
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium chloride 1.3 M -
pH 6.5
260.0 μl of Ammonium chloride (5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
640.0 μl of water (100 v/v)
B11
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium chloride 2.4 M -
pH 6.5
480.0 μl of Ammonium chloride (5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
420.0 μl of water (100 v/v)
B12
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium chloride 3.5 M -
pH 6.5
700.0 μl of Ammonium chloride (5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
200.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 0.2 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
820.0 μl of water (100 v/v)
C2
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 0.7 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
280.0 μl of di-Ammonium hydrogen citrate (2.5 M)
620.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 1.3 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
520.0 μl of di-Ammonium hydrogen citrate (2.5 M)
380.0 μl of water (100 v/v)
C4
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 1.8 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
720.0 μl of di-Ammonium hydrogen citrate (2.5 M)
180.0 μl of water (100 v/v)
C5
  • HEPES 0.1 M pH 7.5
  • di-Ammonium hydrogen citrate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
80.0 μl of di-Ammonium hydrogen citrate (2.5 M)
820.0 μl of water (100 v/v)
C6
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 0.7 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
280.0 μl of di-Ammonium hydrogen citrate (2.5 M)
620.0 μl of water (100 v/v)
C7
  • Sodium acetate 0.1 M pH 4.5
  • di-Ammonium hydrogen citrate 1.3 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
520.0 μl of di-Ammonium hydrogen citrate (2.5 M)
380.0 μl of water (100 v/v)
C8
  • HEPES 0.1 M pH 7.5
  • di-Ammonium hydrogen citrate 1.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
720.0 μl of di-Ammonium hydrogen citrate (2.5 M)
180.0 μl of water (100 v/v)
C9
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium formate 0.2 M -
pH 7
20.0 μl of Ammonium formate (10 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
880.0 μl of water (100 v/v)
C10
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium formate 1.1 M -
pH 7
110.0 μl of Ammonium formate (10 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
790.0 μl of water (100 v/v)
C11
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium formate 2 M -
pH 7
200.0 μl of Ammonium formate (10 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
700.0 μl of water (100 v/v)
C12
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium formate 3 M -
pH 7
300.0 μl of Ammonium formate (10 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
600.0 μl of water (100 v/v)
D1
  • tris chloride BCC 0.1 M pH 8
  • di-Ammonium hydrogen phosphate 0.2 M -
pH 8
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
842.9 μl of water (100 v/v)
D2
  • tris chloride BCC 0.1 M pH 8
  • di-Ammonium hydrogen phosphate 1 M -
pH 8
285.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
614.3 μl of water (100 v/v)
D3
  • tris chloride BCC 0.1 M pH 8
  • di-Ammonium hydrogen phosphate 1.7 M -
pH 8
485.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
414.3 μl of water (100 v/v)
D4
  • tris chloride BCC 0.1 M pH 8
  • di-Ammonium hydrogen phosphate 2.5 M -
pH 8
714.3 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
185.7 μl of water (100 v/v)
D5
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium di-hydrogen phosphate 0.2 M -
pH 4.5
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
820.0 μl of water (100 v/v)
D6
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium di-hydrogen phosphate 0.8 M -
pH 4.5
320.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
D7
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium di-hydrogen phosphate 1.4 M -
pH 4.5
560.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
D8
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium di-hydrogen phosphate 2 M -
pH 4.5
800.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
100.0 μl of water (100 v/v)
D9
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 0.2 M -
pH 4.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
842.9 μl of water (100 v/v)
D10
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 0.8 M -
pH 4.5
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
671.4 μl of water (100 v/v)
D11
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 1.4 M -
pH 4.5
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
D12
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 2 M -
pH 4.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
328.6 μl of water (100 v/v)
E1
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium sulfate 0.2 M -
pH 5.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
842.9 μl of water (100 v/v)
E2
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium sulfate 0.8 M -
pH 5.5
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
671.4 μl of water (100 v/v)
E3
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium sulfate 1.4 M -
pH 5.5
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
E4
  • di-Sodium succinate 0.1 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
328.6 μl of water (100 v/v)
E5
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 0.2 M -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
842.9 μl of water (100 v/v)
E6
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 0.8 M -
pH 6.5
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
671.4 μl of water (100 v/v)
E7
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 1.4 M -
pH 6.5
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of water (100 v/v)
E8
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
328.6 μl of water (100 v/v)
E9
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium sulfate 0.2 M -
pH 7
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
842.9 μl of water (100 v/v)
E10
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium sulfate 0.8 M -
pH 7
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
671.4 μl of water (100 v/v)
E11
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium sulfate 1.4 M -
pH 7
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
500.0 μl of water (100 v/v)
E12
  • Sodium Potassium phosphate 0.1 M pH 7
  • Ammonium sulfate 2 M -
pH 7
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
328.6 μl of water (100 v/v)
F1
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.2 M -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
842.9 μl of water (100 v/v)
F2
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 0.8 M -
pH 7.5
228.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
671.4 μl of water (100 v/v)
F3
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 1.4 M -
pH 7.5
400.0 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of water (100 v/v)
F4
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
328.6 μl of water (100 v/v)
F5
  • di-Sodium succinate 0.1 M pH 5.5
  • di-Ammonium tartrate 0.2 M -
pH 5.5
100.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
800.0 μl of water (100 v/v)
F6
  • di-Sodium succinate 0.1 M pH 5.5
  • di-Ammonium tartrate 0.6 M -
pH 5.5
300.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
600.0 μl of water (100 v/v)
F7
  • di-Sodium succinate 0.1 M pH 5.5
  • di-Ammonium tartrate 1 M -
pH 5.5
500.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
F8
  • di-Sodium succinate 0.1 M pH 5.5
  • di-Ammonium tartrate 1.5 M -
pH 5.5
750.0 μl of di-Ammonium tartrate (2 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
150.0 μl of water (100 v/v)
F9
  • BIS-TRIS 0.1 M pH 6.5
  • di-Ammonium tartrate 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of di-Ammonium tartrate (2 M)
800.0 μl of water (100 v/v)
F10
  • BIS-TRIS 0.1 M pH 6.5
  • di-Ammonium tartrate 0.6 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
300.0 μl of di-Ammonium tartrate (2 M)
600.0 μl of water (100 v/v)
F11
  • BIS-TRIS 0.1 M pH 6.5
  • di-Ammonium tartrate 1 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of di-Ammonium tartrate (2 M)
400.0 μl of water (100 v/v)
F12
  • BIS-TRIS 0.1 M pH 6.5
  • di-Ammonium tartrate 1.5 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
750.0 μl of di-Ammonium tartrate (2 M)
150.0 μl of water (100 v/v)
G1
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Ammonium tartrate 0.2 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
100.0 μl of di-Ammonium tartrate (2 M)
800.0 μl of water (100 v/v)
G2
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Ammonium tartrate 0.6 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
300.0 μl of di-Ammonium tartrate (2 M)
600.0 μl of water (100 v/v)
G3
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Ammonium tartrate 1 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
500.0 μl of di-Ammonium tartrate (2 M)
400.0 μl of water (100 v/v)
G4
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Ammonium tartrate 1.5 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
750.0 μl of di-Ammonium tartrate (2 M)
150.0 μl of water (100 v/v)
G5
  • HEPES 0.1 M pH 7.5
  • di-Ammonium tartrate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of di-Ammonium tartrate (2 M)
800.0 μl of water (100 v/v)
G6
  • HEPES 0.1 M pH 7.5
  • di-Ammonium tartrate 0.6 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of di-Ammonium tartrate (2 M)
600.0 μl of water (100 v/v)
G7
  • HEPES 0.1 M pH 7.5
  • di-Ammonium tartrate 1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of di-Ammonium tartrate (2 M)
400.0 μl of water (100 v/v)
G8
  • HEPES 0.1 M pH 7.5
  • di-Ammonium tartrate 1.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
750.0 μl of di-Ammonium tartrate (2 M)
150.0 μl of water (100 v/v)
G9
  • CHES 0.1 M pH 10
  • di-Potassium hydrogen phosphate 0.2 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
66.7 μl of di-Potassium hydrogen phosphate (3 M)
833.3 μl of water (100 v/v)
G10
  • CHES 0.1 M pH 10
  • di-Potassium hydrogen phosphate 0.7 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
233.3 μl of di-Potassium hydrogen phosphate (3 M)
666.7 μl of water (100 v/v)
G11
  • CHES 0.1 M pH 10
  • di-Potassium hydrogen phosphate 1.3 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
433.3 μl of di-Potassium hydrogen phosphate (3 M)
466.7 μl of water (100 v/v)
G12
  • CHES 0.1 M pH 10
  • di-Potassium hydrogen phosphate 1.8 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
600.0 μl of di-Potassium hydrogen phosphate (3 M)
300.0 μl of water (100 v/v)
H1
  • tris chloride BCC 0.1 M pH 8
  • Sodium acetate 0.2 M -
pH 8
66.7 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
833.3 μl of water (100 v/v)
H2
  • tris chloride BCC 0.1 M pH 8
  • Sodium acetate 0.7 M -
pH 8
233.3 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
666.7 μl of water (100 v/v)
H3
  • tris chloride BCC 0.1 M pH 8
  • Sodium acetate 1.3 M -
pH 8
433.3 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
466.7 μl of water (100 v/v)
H4
  • tris chloride BCC 0.1 M pH 8
  • Sodium acetate 1.8 M -
pH 8
600.0 μl of Sodium acetate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
H5
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium acetate 0.2 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
66.7 μl of Sodium acetate (3 M)
833.3 μl of water (100 v/v)
H6
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium acetate 0.7 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
233.3 μl of Sodium acetate (3 M)
666.7 μl of water (100 v/v)
H7
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium acetate 1.3 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
433.3 μl of Sodium acetate (3 M)
466.7 μl of water (100 v/v)
H8
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium acetate 1.8 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
600.0 μl of Sodium acetate (3 M)
300.0 μl of water (100 v/v)
H9
  • CHES 0.1 M pH 10
  • Sodium acetate 0.2 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
66.7 μl of Sodium acetate (3 M)
833.3 μl of water (100 v/v)
H10
  • CHES 0.1 M pH 10
  • Sodium acetate 0.7 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
233.3 μl of Sodium acetate (3 M)
666.7 μl of water (100 v/v)
H11
  • CHES 0.1 M pH 10
  • Sodium acetate 1.3 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
433.3 μl of Sodium acetate (3 M)
466.7 μl of water (100 v/v)
H12
  • CHES 0.1 M pH 10
  • Sodium acetate 1.8 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
600.0 μl of Sodium acetate (3 M)
300.0 μl of water (100 v/v)