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Supplemental Information

Screen Description

GRAS8

GRAS screens / GRAS8
Created 2016-09-29.

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GRAS8

Barcodes

GRAS8

    Screen Structure

    GRAS8

    Well Count 96
    Number of distinct ingredients 14
    Internal diversity (score mean dist) 0.877

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:20:36.651100 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type GRAS8
    Buffer 160
    Salt 116

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    GRAS8 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 0.2 M -
    		pH 4.5
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    860 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 1.1 M -
    		pH 4.5
    220 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    680 μl of water (100 v/v)
    A3
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 1.9 M -
    		pH 4.5
    380 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    520 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 2.8 M -
    		pH 4.5
    560 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    340 μl of water (100 v/v)
    A5
    • Sodium chloride 0.2 M -
    • di-Sodium succinate 0.1 M pH 5.5
    		pH 5.5
    40 μl of Sodium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    860 μl of water (100 v/v)
    A6
    • Sodium chloride 1.1 M -
    • di-Sodium succinate 0.1 M pH 5.5
    		pH 5.5
    220 μl of Sodium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    680 μl of water (100 v/v)
    A7
    • Sodium chloride 1.9 M -
    • di-Sodium succinate 0.1 M pH 5.5
    		pH 5.5
    380 μl of Sodium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    520 μl of water (100 v/v)
    A8
    • Sodium chloride 2.8 M -
    • di-Sodium succinate 0.1 M pH 5.5
    		pH 5.5
    560 μl of Sodium chloride (5 M)
    100 μl of di-Sodium succinate pH 5.5 (1 M)
    340 μl of water (100 v/v)
    A9
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 0.2 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    40 μl of Sodium chloride (5 M)
    860 μl of water (100 v/v)
    A10
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 1.1 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    220 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    A11
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 1.9 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    380 μl of Sodium chloride (5 M)
    520 μl of water (100 v/v)
    A12
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 2.8 M -
    		pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    B1
    • Sodium Potassium phosphate 0.1 M pH 7
    • Sodium chloride 0.2 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    860 μl of water (100 v/v)
    B2
    • Sodium Potassium phosphate 0.1 M pH 7
    • Sodium chloride 1.1 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    220 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    B3
    • Sodium Potassium phosphate 0.1 M pH 7
    • Sodium chloride 1.9 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    380 μl of Sodium chloride (5 M)
    520 μl of water (100 v/v)
    B4
    • Sodium Potassium phosphate 0.1 M pH 7
    • Sodium chloride 2.8 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    560 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    B5
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 0.2 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    40 μl of Sodium chloride (5 M)
    860 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 1.1 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    220 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    B7
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 1.9 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    380 μl of Sodium chloride (5 M)
    520 μl of water (100 v/v)
    B8
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 2.8 M -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    560 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    B9
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    40 μl of Sodium chloride (5 M)
    860 μl of water (100 v/v)
    B10
    • Sodium chloride 1.1 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    220 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    B11
    • Sodium chloride 1.9 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    380 μl of Sodium chloride (5 M)
    520 μl of water (100 v/v)
    B12
    • Sodium chloride 2.8 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    560 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    C1
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium chloride 0.2 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    40 μl of Sodium chloride (5 M)
    860 μl of water (100 v/v)
    C2
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium chloride 1.1 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    220 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    C3
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium chloride 1.9 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    380 μl of Sodium chloride (5 M)
    520 μl of water (100 v/v)
    C4
    • BIS-TRIS propane 0.1 M pH 9
    • Sodium chloride 2.8 M -
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    560 μl of Sodium chloride (5 M)
    340 μl of water (100 v/v)
    C5
    • CHES 0.1 M pH 10
    • Sodium chloride 0.2 M -
    		pH 10
    40 μl of Sodium chloride (5 M)
    100 μl of CHES pH 10 (1 M)
    860 μl of water (100 v/v)
    C6
    • CHES 0.1 M pH 10
    • Sodium chloride 1.1 M -
    		pH 10
    220 μl of Sodium chloride (5 M)
    100 μl of CHES pH 10 (1 M)
    680 μl of water (100 v/v)
    C7
    • CHES 0.1 M pH 10
    • Sodium chloride 1.9 M -
    		pH 10
    380 μl of Sodium chloride (5 M)
    100 μl of CHES pH 10 (1 M)
    520 μl of water (100 v/v)
    C8
    • CHES 0.1 M pH 10
    • Sodium chloride 2.8 M -
    		pH 10
    560 μl of Sodium chloride (5 M)
    100 μl of CHES pH 10 (1 M)
    340 μl of water (100 v/v)
    C9
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 0.2 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    775 μl of water (100 v/v)
    C10
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 0.6 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    C11
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 1 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    C12
    • tris chloride BCC 0.1 M pH 8
    • tri-Sodium citrate 1.4 M -
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    875 μl of tri-Sodium citrate (1.6 M)
    25 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    775 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.6 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    D3
    • tri-Sodium citrate 1 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 1.4 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    875 μl of tri-Sodium citrate (1.6 M)
    25 μl of water (100 v/v)
    D5
    • tri-Sodium citrate 0.2 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    125 μl of tri-Sodium citrate (1.6 M)
    775 μl of water (100 v/v)
    D6
    • tri-Sodium citrate 0.6 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    375 μl of tri-Sodium citrate (1.6 M)
    525 μl of water (100 v/v)
    D7
    • tri-Sodium citrate 1 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    D8
    • tri-Sodium citrate 1.4 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    875 μl of tri-Sodium citrate (1.6 M)
    25 μl of water (100 v/v)
    D9
    • Sodium formate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    28 μl of Sodium formate (7 M)
    871 μl of water (100 v/v)
    D10
    • Sodium formate 1.5 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    214 μl of Sodium formate (7 M)
    685 μl of water (100 v/v)
    D11
    • Sodium formate 2.7 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    385 μl of Sodium formate (7 M)
    514 μl of water (100 v/v)
    D12
    • Sodium formate 4 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    571 μl of Sodium formate (7 M)
    328 μl of water (100 v/v)
    E1
    • Sodium formate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    28 μl of Sodium formate (7 M)
    871 μl of water (100 v/v)
    E2
    • Sodium formate 1.5 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    214 μl of Sodium formate (7 M)
    685 μl of water (100 v/v)
    E3
    • Sodium formate 2.7 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    385 μl of Sodium formate (7 M)
    514 μl of water (100 v/v)
    E4
    • Sodium formate 4 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of BIS-TRIS propane pH 9 (1 M)
    571 μl of Sodium formate (7 M)
    328 μl of water (100 v/v)
    E5
    • Sodium formate 0.2 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    28 μl of Sodium formate (7 M)
    871 μl of water (100 v/v)
    E6
    • Sodium formate 1.5 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    214 μl of Sodium formate (7 M)
    685 μl of water (100 v/v)
    E7
    • Sodium formate 2.7 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    385 μl of Sodium formate (7 M)
    514 μl of water (100 v/v)
    E8
    • Sodium formate 4 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of CHES pH 10 (1 M)
    571 μl of Sodium formate (7 M)
    328 μl of water (100 v/v)
    E9
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium hydrogen phosphate 0.1 M -
    		pH 8
    100 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    800 μl of water (100 v/v)
    E10
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium hydrogen phosphate 0.2 M -
    		pH 8
    200 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    700 μl of water (100 v/v)
    E11
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium hydrogen phosphate 0.4 M -
    		pH 8
    400 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    E12
    • tris chloride BCC 0.1 M pH 8
    • di-Sodium hydrogen phosphate 0.6 M -
    		pH 8
    600 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    F1
    • di-Sodium hydrogen phosphate 0.1 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    100 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    800 μl of water (100 v/v)
    F2
    • di-Sodium hydrogen phosphate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    200 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    700 μl of water (100 v/v)
    F3
    • di-Sodium hydrogen phosphate 0.4 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    400 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    500 μl of water (100 v/v)
    F4
    • di-Sodium hydrogen phosphate 0.6 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    600 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    300 μl of water (100 v/v)
    F5
    • di-Sodium hydrogen phosphate 0.1 M -
    • CHES 0.1 M pH 10
    		pH 10
    100 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of CHES pH 10 (1 M)
    800 μl of water (100 v/v)
    F6
    • di-Sodium hydrogen phosphate 0.2 M -
    • CHES 0.1 M pH 10
    		pH 10
    200 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of CHES pH 10 (1 M)
    700 μl of water (100 v/v)
    F7
    • di-Sodium hydrogen phosphate 0.4 M -
    • CHES 0.1 M pH 10
    		pH 10
    400 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of CHES pH 10 (1 M)
    500 μl of water (100 v/v)
    F8
    • di-Sodium hydrogen phosphate 0.6 M -
    • CHES 0.1 M pH 10
    		pH 10
    600 μl of di-Sodium hydrogen phosphate (1 M)
    100 μl of CHES pH 10 (1 M)
    300 μl of water (100 v/v)
    F9
    • Sodium acetate 0.1 M pH 4.5
    • Sodium di-hydrogen phosphate 0.2 M -
    		pH 4.5
    50 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    850 μl of water (100 v/v)
    F10
    • Sodium acetate 0.1 M pH 4.5
    • Sodium di-hydrogen phosphate 0.8 M -
    		pH 4.5
    200 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    700 μl of water (100 v/v)
    F11
    • Sodium acetate 0.1 M pH 4.5
    • Sodium di-hydrogen phosphate 1.4 M -
    		pH 4.5
    350 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    550 μl of water (100 v/v)
    F12
    • Sodium acetate 0.1 M pH 4.5
    • Sodium di-hydrogen phosphate 2 M -
    		pH 4.5
    500 μl of Sodium di-hydrogen phosphate (4 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    G1
    • BIS-TRIS 0.1 M pH 6.5
    • di-Sodium tartrate 0.2 M -
    		pH 6.5
    133 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    766 μl of water (100 v/v)
    G2
    • BIS-TRIS 0.1 M pH 6.5
    • di-Sodium tartrate 0.5 M -
    		pH 6.5
    333 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    566 μl of water (100 v/v)
    G3
    • BIS-TRIS 0.1 M pH 6.5
    • di-Sodium tartrate 0.9 M -
    		pH 6.5
    600 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    300 μl of water (100 v/v)
    G4
    • BIS-TRIS 0.1 M pH 6.5
    • di-Sodium tartrate 1.2 M -
    		pH 6.5
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of water (100 v/v)
    G5
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Sodium tartrate 0.2 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    133 μl of di-Sodium tartrate (1.5 M)
    766 μl of water (100 v/v)
    G6
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Sodium tartrate 0.5 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    333 μl of di-Sodium tartrate (1.5 M)
    566 μl of water (100 v/v)
    G7
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Sodium tartrate 0.9 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    600 μl of di-Sodium tartrate (1.5 M)
    300 μl of water (100 v/v)
    G8
    • Sodium Potassium phosphate 0.1 M pH 7
    • di-Sodium tartrate 1.2 M -
    		pH 7
    100 μl of Sodium Potassium phosphate pH 7 (1 M)
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of water (100 v/v)
    G9
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 0.2 M -
    		pH 7.5
    133 μl of di-Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    766 μl of water (100 v/v)
    G10
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 0.5 M -
    		pH 7.5
    333 μl of di-Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    566 μl of water (100 v/v)
    G11
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 0.9 M -
    		pH 7.5
    600 μl of di-Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of water (100 v/v)
    G12
    • HEPES 0.1 M pH 7.5
    • di-Sodium tartrate 1.2 M -
    		pH 7.5
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    H1
    • di-Sodium tartrate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    133 μl of di-Sodium tartrate (1.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    766 μl of water (100 v/v)
    H2
    • di-Sodium tartrate 0.5 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    333 μl of di-Sodium tartrate (1.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    566 μl of water (100 v/v)
    H3
    • di-Sodium tartrate 0.9 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    600 μl of di-Sodium tartrate (1.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    H4
    • di-Sodium tartrate 1.2 M -
    • tris chloride BCC 0.1 M pH 8
    		pH 8
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of water (100 v/v)
    H5
    • di-Sodium tartrate 0.2 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    133 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    766 μl of water (100 v/v)
    H6
    • di-Sodium tartrate 0.5 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    333 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    566 μl of water (100 v/v)
    H7
    • di-Sodium tartrate 0.9 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    600 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    300 μl of water (100 v/v)
    H8
    • di-Sodium tartrate 1.2 M -
    • BIS-TRIS propane 0.1 M pH 9
    		pH 9
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS propane pH 9 (1 M)
    100 μl of water (100 v/v)
    H9
    • di-Sodium tartrate 0.2 M -
    • CHES 0.1 M pH 10
    		pH 10
    133 μl of di-Sodium tartrate (1.5 M)
    100 μl of CHES pH 10 (1 M)
    766 μl of water (100 v/v)
    H10
    • di-Sodium tartrate 0.5 M -
    • CHES 0.1 M pH 10
    		pH 10
    333 μl of di-Sodium tartrate (1.5 M)
    100 μl of CHES pH 10 (1 M)
    566 μl of water (100 v/v)
    H11
    • di-Sodium tartrate 0.9 M -
    • CHES 0.1 M pH 10
    		pH 10
    600 μl of di-Sodium tartrate (1.5 M)
    100 μl of CHES pH 10 (1 M)
    300 μl of water (100 v/v)
    H12
    • di-Sodium tartrate 1.2 M -
    • CHES 0.1 M pH 10
    		pH 10
    800 μl of di-Sodium tartrate (1.5 M)
    100 μl of CHES pH 10 (1 M)
    100 μl of water (100 v/v)