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Supplemental Information

Screen Description

GRAS8

GRAS screens / GRAS8
Created 2016-09-29.

Export

GRAS8

Barcodes

GRAS8

No barcodes found.

Screen Structure

GRAS8

Well Count 96
Number of distinct ingredients 14
Internal diversity (score mean dist) 0.877

Screen Composition

More information about screen composition chart.
2025-08-11T20:26:42.543718 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type GRAS8
Buffer 160
Salt 116

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
GRAS8 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
  • Sodium chloride 0.2 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.5
  • Sodium chloride 1.1 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
A3
  • Sodium acetate 0.1 M pH 4.5
  • Sodium chloride 1.9 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 4.5
  • Sodium chloride 2.8 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
A5
  • Sodium chloride 0.2 M -
  • di-Sodium succinate 0.1 M pH 5.5
pH 5.5
40.0 μl of Sodium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
860.0 μl of water (100 v/v)
A6
  • Sodium chloride 1.1 M -
  • di-Sodium succinate 0.1 M pH 5.5
pH 5.5
220.0 μl of Sodium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
680.0 μl of water (100 v/v)
A7
  • Sodium chloride 1.9 M -
  • di-Sodium succinate 0.1 M pH 5.5
pH 5.5
380.0 μl of Sodium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
520.0 μl of water (100 v/v)
A8
  • Sodium chloride 2.8 M -
  • di-Sodium succinate 0.1 M pH 5.5
pH 5.5
560.0 μl of Sodium chloride (5 M)
100.0 μl of di-Sodium succinate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
A9
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
A10
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 1.1 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
A11
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 1.9 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
A12
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 2.8 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
B1
  • Sodium Potassium phosphate 0.1 M pH 7
  • Sodium chloride 0.2 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
B2
  • Sodium Potassium phosphate 0.1 M pH 7
  • Sodium chloride 1.1 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
B3
  • Sodium Potassium phosphate 0.1 M pH 7
  • Sodium chloride 1.9 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
B4
  • Sodium Potassium phosphate 0.1 M pH 7
  • Sodium chloride 2.8 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
B5
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 1.1 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
B7
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 1.9 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
B8
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 2.8 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
B9
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
860.0 μl of water (100 v/v)
B10
  • Sodium chloride 1.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
220.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
680.0 μl of water (100 v/v)
B11
  • Sodium chloride 1.9 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
380.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
520.0 μl of water (100 v/v)
B12
  • Sodium chloride 2.8 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
560.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
340.0 μl of water (100 v/v)
C1
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium chloride 0.2 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
C2
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium chloride 1.1 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
C3
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium chloride 1.9 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
C4
  • BIS-TRIS propane 0.1 M pH 9
  • Sodium chloride 2.8 M -
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
C5
  • CHES 0.1 M pH 10
  • Sodium chloride 0.2 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
40.0 μl of Sodium chloride (5 M)
860.0 μl of water (100 v/v)
C6
  • CHES 0.1 M pH 10
  • Sodium chloride 1.1 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
220.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
C7
  • CHES 0.1 M pH 10
  • Sodium chloride 1.9 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
380.0 μl of Sodium chloride (5 M)
520.0 μl of water (100 v/v)
C8
  • CHES 0.1 M pH 10
  • Sodium chloride 2.8 M -
pH 10
100.0 μl of CHES pH 10 (1 M)
560.0 μl of Sodium chloride (5 M)
340.0 μl of water (100 v/v)
C9
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 0.2 M -
pH 8
125.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
775.0 μl of water (100 v/v)
C10
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 0.6 M -
pH 8
375.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
525.0 μl of water (100 v/v)
C11
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 1 M -
pH 8
625.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
275.0 μl of water (100 v/v)
C12
  • tris chloride BCC 0.1 M pH 8
  • tri-Sodium citrate 1.4 M -
pH 8
875.0 μl of tri-Sodium citrate (1.6 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
25.0 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.2 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
775.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.6 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
375.0 μl of tri-Sodium citrate (1.6 M)
525.0 μl of water (100 v/v)
D3
  • tri-Sodium citrate 1 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
D4
  • tri-Sodium citrate 1.4 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
D5
  • tri-Sodium citrate 0.2 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
125.0 μl of tri-Sodium citrate (1.6 M)
775.0 μl of water (100 v/v)
D6
  • tri-Sodium citrate 0.6 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
375.0 μl of tri-Sodium citrate (1.6 M)
525.0 μl of water (100 v/v)
D7
  • tri-Sodium citrate 1 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
D8
  • tri-Sodium citrate 1.4 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
D9
  • Sodium formate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
28.6 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
871.4 μl of water (100 v/v)
D10
  • Sodium formate 1.5 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
214.3 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
685.7 μl of water (100 v/v)
D11
  • Sodium formate 2.7 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
385.7 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
514.3 μl of water (100 v/v)
D12
  • Sodium formate 4 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
571.4 μl of Sodium formate (7 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
328.6 μl of water (100 v/v)
E1
  • Sodium formate 0.2 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
28.6 μl of Sodium formate (7 M)
871.4 μl of water (100 v/v)
E2
  • Sodium formate 1.5 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
214.3 μl of Sodium formate (7 M)
685.7 μl of water (100 v/v)
E3
  • Sodium formate 2.7 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
385.7 μl of Sodium formate (7 M)
514.3 μl of water (100 v/v)
E4
  • Sodium formate 4 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
571.4 μl of Sodium formate (7 M)
328.6 μl of water (100 v/v)
E5
  • Sodium formate 0.2 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
28.6 μl of Sodium formate (7 M)
871.4 μl of water (100 v/v)
E6
  • Sodium formate 1.5 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
214.3 μl of Sodium formate (7 M)
685.7 μl of water (100 v/v)
E7
  • Sodium formate 2.7 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
385.7 μl of Sodium formate (7 M)
514.3 μl of water (100 v/v)
E8
  • Sodium formate 4 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
571.4 μl of Sodium formate (7 M)
328.6 μl of water (100 v/v)
E9
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium hydrogen phosphate 0.1 M -
pH 8
100.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
800.0 μl of water (100 v/v)
E10
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium hydrogen phosphate 0.2 M -
pH 8
200.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
700.0 μl of water (100 v/v)
E11
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium hydrogen phosphate 0.4 M -
pH 8
400.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
E12
  • tris chloride BCC 0.1 M pH 8
  • di-Sodium hydrogen phosphate 0.6 M -
pH 8
600.0 μl of di-Sodium hydrogen phosphate (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
F1
  • di-Sodium hydrogen phosphate 0.1 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
100.0 μl of di-Sodium hydrogen phosphate (1 M)
800.0 μl of water (100 v/v)
F2
  • di-Sodium hydrogen phosphate 0.2 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
200.0 μl of di-Sodium hydrogen phosphate (1 M)
700.0 μl of water (100 v/v)
F3
  • di-Sodium hydrogen phosphate 0.4 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
400.0 μl of di-Sodium hydrogen phosphate (1 M)
500.0 μl of water (100 v/v)
F4
  • di-Sodium hydrogen phosphate 0.6 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
600.0 μl of di-Sodium hydrogen phosphate (1 M)
300.0 μl of water (100 v/v)
F5
  • di-Sodium hydrogen phosphate 0.1 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
100.0 μl of di-Sodium hydrogen phosphate (1 M)
800.0 μl of water (100 v/v)
F6
  • di-Sodium hydrogen phosphate 0.2 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
200.0 μl of di-Sodium hydrogen phosphate (1 M)
700.0 μl of water (100 v/v)
F7
  • di-Sodium hydrogen phosphate 0.4 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
400.0 μl of di-Sodium hydrogen phosphate (1 M)
500.0 μl of water (100 v/v)
F8
  • di-Sodium hydrogen phosphate 0.6 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
600.0 μl of di-Sodium hydrogen phosphate (1 M)
300.0 μl of water (100 v/v)
F9
  • Sodium acetate 0.1 M pH 4.5
  • Sodium di-hydrogen phosphate 0.2 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
50.0 μl of Sodium di-hydrogen phosphate (4 M)
850.0 μl of water (100 v/v)
F10
  • Sodium acetate 0.1 M pH 4.5
  • Sodium di-hydrogen phosphate 0.8 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
200.0 μl of Sodium di-hydrogen phosphate (4 M)
700.0 μl of water (100 v/v)
F11
  • Sodium acetate 0.1 M pH 4.5
  • Sodium di-hydrogen phosphate 1.4 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
350.0 μl of Sodium di-hydrogen phosphate (4 M)
550.0 μl of water (100 v/v)
F12
  • Sodium acetate 0.1 M pH 4.5
  • Sodium di-hydrogen phosphate 2 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of Sodium di-hydrogen phosphate (4 M)
400.0 μl of water (100 v/v)
G1
  • BIS-TRIS 0.1 M pH 6.5
  • di-Sodium tartrate 0.2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
133.3 μl of di-Sodium tartrate (1.5 M)
766.7 μl of water (100 v/v)
G2
  • BIS-TRIS 0.1 M pH 6.5
  • di-Sodium tartrate 0.5 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
333.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
G3
  • BIS-TRIS 0.1 M pH 6.5
  • di-Sodium tartrate 0.9 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of di-Sodium tartrate (1.5 M)
300.0 μl of water (100 v/v)
G4
  • BIS-TRIS 0.1 M pH 6.5
  • di-Sodium tartrate 1.2 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)
G5
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Sodium tartrate 0.2 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
133.3 μl of di-Sodium tartrate (1.5 M)
766.7 μl of water (100 v/v)
G6
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Sodium tartrate 0.5 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
333.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
G7
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Sodium tartrate 0.9 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
600.0 μl of di-Sodium tartrate (1.5 M)
300.0 μl of water (100 v/v)
G8
  • Sodium Potassium phosphate 0.1 M pH 7
  • di-Sodium tartrate 1.2 M -
pH 7
100.0 μl of Sodium Potassium phosphate pH 7 (1 M)
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)
G9
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
133.3 μl of di-Sodium tartrate (1.5 M)
766.7 μl of water (100 v/v)
G10
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 0.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
333.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
G11
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 0.9 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of di-Sodium tartrate (1.5 M)
300.0 μl of water (100 v/v)
G12
  • HEPES 0.1 M pH 7.5
  • di-Sodium tartrate 1.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)
H1
  • di-Sodium tartrate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
133.3 μl of di-Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
766.7 μl of water (100 v/v)
H2
  • di-Sodium tartrate 0.5 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
333.3 μl of di-Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
566.7 μl of water (100 v/v)
H3
  • di-Sodium tartrate 0.9 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
600.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
H4
  • di-Sodium tartrate 1.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)
H5
  • di-Sodium tartrate 0.2 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
133.3 μl of di-Sodium tartrate (1.5 M)
766.7 μl of water (100 v/v)
H6
  • di-Sodium tartrate 0.5 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
333.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
H7
  • di-Sodium tartrate 0.9 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
600.0 μl of di-Sodium tartrate (1.5 M)
300.0 μl of water (100 v/v)
H8
  • di-Sodium tartrate 1.2 M -
  • BIS-TRIS propane 0.1 M pH 9
pH 9
100.0 μl of BIS-TRIS propane pH 9 (1 M)
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)
H9
  • di-Sodium tartrate 0.2 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
133.3 μl of di-Sodium tartrate (1.5 M)
766.7 μl of water (100 v/v)
H10
  • di-Sodium tartrate 0.5 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
333.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
H11
  • di-Sodium tartrate 0.9 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
600.0 μl of di-Sodium tartrate (1.5 M)
300.0 μl of water (100 v/v)
H12
  • di-Sodium tartrate 1.2 M -
  • CHES 0.1 M pH 10
pH 10
100.0 μl of CHES pH 10 (1 M)
800.0 μl of di-Sodium tartrate (1.5 M)
100.0 μl of water (100 v/v)