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Supplemental Information

Screen Description

Analytic-Crystallizer

Microlytic-Anatrace / Analytic-Crystallizer
Created 2016-12-06.

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Analytic-Crystallizer

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Screen Structure

Analytic-Crystallizer

Well Count 96
Number of distinct ingredients 34
Internal diversity (score mean dist) 0.936

Screen Composition

2025-08-16T04:27:13.984414 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Analytic-Crystallizer
Buffer 80
Cryoprotectant 10
Organic (non-volatile) 22
Polymer 95
Salt 102

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Analytic-Crystallizer Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.21 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 400 48 v/v -
  • Glycerol 1.12 v/v -
pH 4.5
11.2 μl of Glycerol (100 v/v)
480.0 μl of Polyethylene glycol 400 (100 v/v)
105.0 μl of Sodium acetate (2 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
303.8 μl of water (100 v/v)
A2
  • Potassium chloride 0.12 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 400 32 v/v -
pH 5.5
320.0 μl of Polyethylene glycol 400 (100 v/v)
30.0 μl of Potassium chloride (4 M)
100.0 μl of Sodium acetate pH 5.5 (1 M)
550.0 μl of water (100 v/v)
A3
  • di-Sodium malonate 0.23 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 400 46 v/v -
pH 5.5
460.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
67.6 μl of di-Sodium malonate pH 7 (3.4 M)
372.4 μl of water (100 v/v)
A4
  • Magnesium acetate 0.29 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 42 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
290.0 μl of Magnesium acetate (1 M)
420.0 μl of Polyethylene glycol 400 (100 v/v)
190.0 μl of water (100 v/v)
A5
  • Sodium acetate 0.18 M -
  • Polyethylene glycol 400 45 v/v -
  • Trimethylamine N-oxide 0.45 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
90.0 μl of Sodium acetate (2 M)
150.0 μl of Trimethylamine N-oxide (3 M)
210.0 μl of water (100 v/v)
A6
  • Lithium nitrate 0.29 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 28 v/v -
pH 8.5
36.2 μl of Lithium nitrate (8 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
583.8 μl of water (100 v/v)
A7
  • Magnesium chloride 0.17 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 400 36 v/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
85.0 μl of Magnesium chloride (2 M)
360.0 μl of Polyethylene glycol 400 (100 v/v)
455.0 μl of water (100 v/v)
A8
  • Magnesium sulfate 0.12 M -
  • Polyethylene glycol 400 36 v/v -
  • Glycerol 3.67 v/v -
NA
36.7 μl of Glycerol (100 v/v)
60.0 μl of Magnesium sulfate (2 M)
360.0 μl of Polyethylene glycol 400 (100 v/v)
543.3 μl of water (100 v/v)
A9
  • Ammonium sulfate 0.17 M -
  • Polyethylene glycol 400 47 v/v -
NA
56.7 μl of Ammonium sulfate (3 M)
470.0 μl of Polyethylene glycol 400 (100 v/v)
473.3 μl of water (100 v/v)
A10
  • di-Sodium malonate 0.22 M -
  • Polyethylene glycol 400 41 v/v -
NA
410.0 μl of Polyethylene glycol 400 (100 v/v)
64.7 μl of di-Sodium malonate pH 4 (3.4 M)
525.3 μl of water (100 v/v)
A11
  • Sodium sulfate 0.04 M -
  • Polyethylene glycol 400 31 v/v -
NA
310.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium sulfate (1 M)
650.0 μl of water (100 v/v)
A12
  • Sodium sulfate 0.24 M -
  • Polyethylene glycol 400 32 v/v -
NA
320.0 μl of Polyethylene glycol 400 (100 v/v)
240.0 μl of Sodium sulfate (1 M)
440.0 μl of water (100 v/v)
B1
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Glycerol 3.22 v/v -
pH 4.5
32.2 μl of Glycerol (100 v/v)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
567.8 μl of water (100 v/v)
B2
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 550 37 v/v -
pH 5.5
370.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
530.0 μl of water (100 v/v)
B3
  • Calcium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 550 25 v/v -
pH 5.5
100.0 μl of Calcium chloride (2 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
550.0 μl of water (100 v/v)
B4
  • Magnesium sulfate 0.17 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 33 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
85.0 μl of Magnesium sulfate (2 M)
330.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
485.0 μl of water (100 v/v)
B5
  • Sodium bromide 0.14 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 38 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
380.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
28.0 μl of Sodium bromide (5 M)
492.0 μl of water (100 v/v)
B6
  • Calcium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 39 v/v -
pH 7.5
50.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
390.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
460.0 μl of water (100 v/v)
B7
  • Ammonium nitrate 0.22 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 550 34 v/v -
pH 8.5
22.0 μl of Ammonium nitrate (10 M)
340.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
538.0 μl of water (100 v/v)
B8
  • Lithium chloride 0.06 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol monomethyl ether 550 23 v/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
12.0 μl of Lithium chloride (5 M)
230.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
658.0 μl of water (100 v/v)
B9
  • Polyethylene glycol monomethyl ether 550 29 v/v -
  • Potassium di-hydrogen phosphate 0.29 M -
NA
290.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
193.3 μl of Potassium di-hydrogen phosphate (1.5 M)
516.7 μl of water (100 v/v)
B10
  • Sodium bromide 0.28 M -
  • Polyethylene glycol monomethyl ether 550 23 v/v -
NA
230.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
56.0 μl of Sodium bromide (5 M)
714.0 μl of water (100 v/v)
B11
  • Polyethylene glycol monomethyl ether 550 22 v/v -
NA
220.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
780.0 μl of water (100 v/v)
B12
  • Calcium chloride 0.2 M -
  • Polyethylene glycol monomethyl ether 550 30 v/v -
NA
100.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
600.0 μl of water (100 v/v)
C1
  • di-Sodium malonate 0.22 M -
  • Polyethylene glycol monomethyl ether 550 32 v/v -
NA
320.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
64.7 μl of di-Sodium malonate pH 7 (3.4 M)
615.3 μl of water (100 v/v)
C2
  • Magnesium acetate 0.16 M -
  • Polyethylene glycol monomethyl ether 550 38 v/v -
NA
160.0 μl of Magnesium acetate (1 M)
380.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
460.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.25 M -
  • Polyethylene glycol monomethyl ether 550 33 v/v -
NA
330.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
125.0 μl of Sodium acetate (2 M)
545.0 μl of water (100 v/v)
C4
  • Potassium chloride 0.19 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 25 w/v -
pH 4.5
500.0 μl of Polyethylene glycol 1,000 (50 w/v)
47.5 μl of Potassium chloride (4 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
352.5 μl of water (100 v/v)
C5
  • Ammonium nitrate 0.19 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 1,000 35 w/v -
pH 4.5
19.0 μl of Ammonium nitrate (10 M)
700.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
181.0 μl of water (100 v/v)
C6
  • Ammonium nitrate 0.14 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 1,000 27 w/v -
pH 5.5
14.0 μl of Ammonium nitrate (10 M)
540.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
346.0 μl of water (100 v/v)
C7
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 1,000 34 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
680.0 μl of Polyethylene glycol 1,000 (50 w/v)
220.0 μl of water (100 v/v)
C8
  • Ammonium nitrate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 20 w/v -
pH 7.5
20.0 μl of Ammonium nitrate (10 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 1,000 (50 w/v)
480.0 μl of water (100 v/v)
C9
  • di-Sodium malonate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 1,000 28 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
29.4 μl of di-Sodium malonate pH 7 (3.4 M)
310.6 μl of water (100 v/v)
C10
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,000 22 w/v -
pH 8.5
440.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
460.0 μl of water (100 v/v)
C11
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,000 33 w/v -
pH 8.5
660.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
240.0 μl of water (100 v/v)
C12
  • Potassium chloride 0.18 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 1,000 35 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
700.0 μl of Polyethylene glycol 1,000 (50 w/v)
45.0 μl of Potassium chloride (4 M)
155.0 μl of water (100 v/v)
D1
  • Lithium chloride 0.29 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 1,000 28 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
58.0 μl of Lithium chloride (5 M)
560.0 μl of Polyethylene glycol 1,000 (50 w/v)
282.0 μl of water (100 v/v)
D2
  • Lithium nitrate 0.24 M -
  • Polyethylene glycol 1,000 19 w/v -
NA
30.0 μl of Lithium nitrate (8 M)
380.0 μl of Polyethylene glycol 1,000 (50 w/v)
590.0 μl of water (100 v/v)
D3
  • Ammonium sulfate 0.27 M -
  • Polyethylene glycol 1,000 33 w/v -
NA
90.0 μl of Ammonium sulfate (3 M)
660.0 μl of Polyethylene glycol 1,000 (50 w/v)
250.0 μl of water (100 v/v)
D4
  • Sodium bromide 0.21 M -
  • Polyethylene glycol 1,000 29 w/v -
  • Glycerol 3.8 v/v -
NA
38.0 μl of Glycerol (100 v/v)
580.0 μl of Polyethylene glycol 1,000 (50 w/v)
42.0 μl of Sodium bromide (5 M)
340.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 1,000 32 w/v -
  • Glycerol 2.35 v/v -
NA
23.5 μl of Glycerol (100 v/v)
640.0 μl of Polyethylene glycol 1,000 (50 w/v)
336.5 μl of water (100 v/v)
D6
  • Potassium chloride 0.18 M -
  • Polyethylene glycol 1,000 35 w/v -
  • Glycerol 4.16 v/v -
NA
41.6 μl of Glycerol (100 v/v)
700.0 μl of Polyethylene glycol 1,000 (50 w/v)
45.0 μl of Potassium chloride (4 M)
213.4 μl of water (100 v/v)
D7
  • Zinc acetate 0.02 M -
  • Polyethylene glycol 1,000 36 w/v -
NA
720.0 μl of Polyethylene glycol 1,000 (50 w/v)
10.0 μl of Zinc acetate (2 M)
270.0 μl of water (100 v/v)
D8
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
933.3 μl of water (100 v/v)
D9
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 34 w/v -
  • Trimethylamine N-oxide 0.54 M -
pH 4.5
680.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
180.0 μl of Trimethylamine N-oxide (3 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
40.0 μl of water (100 v/v)
D10
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 4.5
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
340.0 μl of water (100 v/v)
D11
  • Zinc acetate 0.02 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 2,000 26 w/v -
pH 4.5
520.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
10.0 μl of Zinc acetate (2 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
370.0 μl of water (100 v/v)
D12
  • Sodium acetate 0.1 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 23 w/v -
pH 5.5
460.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
50.0 μl of Sodium acetate (2 M)
390.0 μl of water (100 v/v)
E1
  • Sodium nitrate 0.15 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 29 w/v -
pH 5.5
580.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
21.4 μl of Sodium nitrate (7 M)
298.6 μl of water (100 v/v)
E2
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 5.5
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
340.0 μl of water (100 v/v)
E3
  • Sodium sulfate 0.13 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 26 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
520.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
130.0 μl of Sodium sulfate (1 M)
250.0 μl of water (100 v/v)
E4
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 18 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
360.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
540.0 μl of water (100 v/v)
E5
  • Lithium chloride 0.29 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 29 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
58.0 μl of Lithium chloride (5 M)
580.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
262.0 μl of water (100 v/v)
E6
  • Lithium nitrate 0.28 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 8.5
35.0 μl of Lithium nitrate (8 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
525.0 μl of water (100 v/v)
E7
  • Zinc acetate 0.02 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 34 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
680.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
10.0 μl of Zinc acetate (2 M)
210.0 μl of water (100 v/v)
E8
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol monomethyl ether 2,000 29 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
580.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
320.0 μl of water (100 v/v)
E9
  • Lithium sulfate 0.12 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
60.0 μl of Lithium sulfate (2 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
500.0 μl of water (100 v/v)
E10
  • Magnesium sulfate 0.27 M -
  • Polyethylene glycol monomethyl ether 2,000 18 w/v -
NA
135.0 μl of Magnesium sulfate (2 M)
360.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
505.0 μl of water (100 v/v)
E11
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol monomethyl ether 2,000 22 w/v -
NA
100.0 μl of Magnesium chloride (2 M)
440.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
460.0 μl of water (100 v/v)
E12
  • Ammonium sulfate 0.21 M -
  • Polyethylene glycol monomethyl ether 2,000 31 w/v -
NA
70.0 μl of Ammonium sulfate (3 M)
620.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
310.0 μl of water (100 v/v)
F1
  • Magnesium chloride 0.17 M -
  • Polyethylene glycol monomethyl ether 2,000 29 w/v -
NA
85.0 μl of Magnesium chloride (2 M)
580.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
335.0 μl of water (100 v/v)
F2
  • Calcium chloride 0.11 M -
  • Polyethylene glycol monomethyl ether 2,000 26 w/v -
NA
55.0 μl of Calcium chloride (2 M)
520.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
425.0 μl of water (100 v/v)
F3
  • Magnesium chloride 0.16 M -
  • Polyethylene glycol monomethyl ether 2,000 27 w/v -
NA
80.0 μl of Magnesium chloride (2 M)
540.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
380.0 μl of water (100 v/v)
F4
  • Sodium acetate 0.23 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
115.0 μl of Sodium acetate (2 M)
285.0 μl of water (100 v/v)
F5
  • Sodium acetate 0.13 M -
  • Polyethylene glycol monomethyl ether 2,000 27 w/v -
NA
540.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
65.0 μl of Sodium acetate (2 M)
395.0 μl of water (100 v/v)
F6
  • Magnesium chloride 0.24 M -
  • Polyethylene glycol monomethyl ether 2,000 33 w/v -
NA
120.0 μl of Magnesium chloride (2 M)
660.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
220.0 μl of water (100 v/v)
F7
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 16 w/v -
pH 4.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
580.0 μl of water (100 v/v)
F8
  • Magnesium chloride 0.21 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 14 w/v -
pH 4.5
105.0 μl of Magnesium chloride (2 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
515.0 μl of water (100 v/v)
F9
  • Ammonium nitrate 0.12 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 16 w/v -
pH 5.5
12.0 μl of Ammonium nitrate (10 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
568.0 μl of water (100 v/v)
F10
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
F11
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 16 w/v -
  • Trimethylamine N-oxide 0.87 M -
pH 5.5
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
290.0 μl of Trimethylamine N-oxide (3 M)
290.0 μl of water (100 v/v)
F12
  • Magnesium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol 4,000 20 w/v -
pH 5.5
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
G1
  • Sodium acetate 0.29 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 13 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
145.0 μl of Sodium acetate (2 M)
495.0 μl of water (100 v/v)
G2
  • Lithium nitrate 0.05 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 19 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
6.2 μl of Lithium nitrate (8 M)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
513.8 μl of water (100 v/v)
G3
  • Zinc acetate 0.02 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 4,000 16 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
10.0 μl of Zinc acetate (2 M)
570.0 μl of water (100 v/v)
G4
  • Ammonium nitrate 0.27 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 17 w/v -
pH 7.5
27.0 μl of Ammonium nitrate (10 M)
100.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
533.0 μl of water (100 v/v)
G5
  • Ammonium nitrate 0.15 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 13 w/v -
  • Trimethylamine N-oxide 0.42 M -
pH 7.5
15.0 μl of Ammonium nitrate (10 M)
100.0 μl of HEPES pH 7.5 (1 M)
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
140.0 μl of Trimethylamine N-oxide (3 M)
485.0 μl of water (100 v/v)
G6
  • Lithium sulfate 0.11 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 13 w/v -
  • Glycerol 1.67 v/v -
pH 8.5
16.7 μl of Glycerol (100 v/v)
55.0 μl of Lithium sulfate (2 M)
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
568.3 μl of water (100 v/v)
G7
  • Lithium acetate 0.24 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 12 w/v -
pH 8.5
48.0 μl of Lithium acetate (5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
612.0 μl of water (100 v/v)
G8
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 21 w/v -
pH 8.5
420.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
480.0 μl of water (100 v/v)
G9
  • di-Potassium hydrogen phosphate 0.27 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 4,000 18 w/v -
pH 8.5
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
67.5 μl of di-Potassium hydrogen phosphate (4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
472.5 μl of water (100 v/v)
G10
  • Sodium sulfate 0.2 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 4,000 14 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
420.0 μl of water (100 v/v)
G11
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol 4,000 20 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
500.0 μl of water (100 v/v)
G12
  • Sodium bromide 0.28 M -
  • Polyethylene glycol 4,000 23 w/v -
NA
460.0 μl of Polyethylene glycol 4,000 (50 w/v)
56.0 μl of Sodium bromide (5 M)
484.0 μl of water (100 v/v)
H1
  • Magnesium chloride 0.21 M -
  • Polyethylene glycol 4,000 13 w/v -
  • Glycerol 1.37 v/v -
NA
13.7 μl of Glycerol (100 v/v)
105.0 μl of Magnesium chloride (2 M)
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
621.3 μl of water (100 v/v)
H2
  • Potassium thiocyanate 0.23 M -
  • Polyethylene glycol 4,000 20 w/v -
  • Glycerol 1.11 v/v -
NA
11.1 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
28.8 μl of Potassium thiocyanate (8 M)
560.1 μl of water (100 v/v)
H3
  • Magnesium sulfate 0.22 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
pH 4.5
110.0 μl of Magnesium sulfate (2 M)
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
430.0 μl of water (100 v/v)
H4
  • Lithium nitrate 0.25 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 17 w/v -
  • Glycerol 4.57 v/v -
pH 5.5
45.7 μl of Glycerol (100 v/v)
31.2 μl of Lithium nitrate (8 M)
340.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
483.0 μl of water (100 v/v)
H5
  • Sodium sulfate 0.22 M -
  • Sodium acetate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 5,000 18 w/v -
  • Trimethylamine N-oxide 0.47 M -
pH 5.5
360.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of Sodium acetate pH 5.5 (1 M)
220.0 μl of Sodium sulfate (1 M)
156.7 μl of Trimethylamine N-oxide (3 M)
163.3 μl of water (100 v/v)
H6
  • Lithium sulfate 0.08 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
40.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
460.0 μl of water (100 v/v)
H7
  • Ammonium sulfate 0.18 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 5,000 12 w/v -
pH 7.5
60.0 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
600.0 μl of water (100 v/v)
H8
  • Sodium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
200.0 μl of Sodium sulfate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H9
  • Lithium nitrate 0.15 M -
  • CAPSO 0.1 M pH 9.5
  • Polyethylene glycol monomethyl ether 5,000 15 w/v -
pH 9.5
100.0 μl of CAPSO pH 9.5 (1 M)
18.8 μl of Lithium nitrate (8 M)
300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
581.2 μl of water (100 v/v)
H10
  • Calcium chloride 0.05 M -
  • Polyethylene glycol monomethyl ether 5,000 15 w/v -
NA
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
675.0 μl of water (100 v/v)
H11
  • Zinc acetate 0.02 M -
  • Polyethylene glycol monomethyl ether 5,000 14 w/v -
NA
280.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
10.0 μl of Zinc acetate (2 M)
710.0 μl of water (100 v/v)
H12
  • Polyethylene glycol monomethyl ether 5,000 16 w/v -
NA
320.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
680.0 μl of water (100 v/v)