C6 - Screen Contents - GRAS Additive

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Supplemental Information

Screen Description

GRAS Additive

GRAS screens / GRAS Additive

Created 2017-07-18.

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GRAS Additive

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GRAS Additive

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Screen Structure

GRAS Additive

Well Count	96
Number of distinct ingredients	109
Internal diversity (score mean dist)	1.000

Screen Composition

More information about screen composition chart.

ingredient type	GRAS Additive
Buffer	2
Complex ingredient	1
Cryoprotectant	1
Detergent	7
Organic (non-volatile)	1
Polymer	1
Salt	4

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

GRAS Additive Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Na-Benzoyl-L-arginine ethyl ester hydrochloride 30 mM - 🔍	NA	300.0 μl of Na-Benzoyl-L-arginine ethyl ester hydrochloride (100 mM) 700.0 μl of water (100 v/v)
A2	L-Carnosine 40 mM - 🔍	NA	400.0 μl of L-Carnosine (100 mM) 600.0 μl of water (100 v/v)
A3	Choline chloride 70 mM - 🔍	NA	350.0 μl of Choline chloride (200 mM) 650.0 μl of water (100 v/v)
A4	Histamine dihydrochloride 50 mM - 🔍	NA	500.0 μl of Histamine dihydrochloride (100 mM) 500.0 μl of water (100 v/v)
A5	Sarcosine 110 mM - 🔍	NA	550.0 μl of Sarcosine (200 mM) 450.0 μl of water (100 v/v)
A6	L-Aspartic acid 0.05 w/v - L-Glutamic acid 0.05 w/v - 🔍	NA	50.0 μl of L-Aspartic acid (1 w/v) 50.0 μl of L-Glutamic acid (1 w/v) 900.0 μl of water (100 v/v)
A7	Glycine 0.5 w/v - L-Proline 0.5 w/v - 🔍	NA	500.0 μl of Glycine (1 w/v) 500.0 μl of L-Proline (1 w/v)
A8	L-Arginine 0.33 w/v - L-Histidine 0.33 w/v - L-Lysine 0.33 w/v - 🔍	NA	330.0 μl of L-Arginine (1 w/v) 330.0 μl of L-Histidine (1 w/v) 330.0 μl of L-Lysine (1 w/v) 10.0 μl of water (100 v/v)
A9	L-Asparagine 0.25 w/v - L-Glutamine 0.25 w/v - L-Serine 0.25 w/v - L-Threonine 0.25 w/v - 🔍	NA	250.0 μl of L-Asparagine (1 w/v) 250.0 μl of L-Glutamine (1 w/v) 250.0 μl of L-Serine (1 w/v) 250.0 μl of L-Threonine (1 w/v)
A10	L-Alanine 0.0125 w/v - L-Isoleucine 0.0125 w/v - L-Leucine 0.0125 w/v - L-Methionine 0.0125 w/v - L-Phenylalanine 0.0125 mM - L-Tyrosine 0.0125 w/v - L-Tryptophan 0.0125 w/v - L-Valine 0.0125 w/v - 🔍	NA	12.5 μl of L-Alanine (1 w/v) 12.5 μl of L-Isoleucine (1 w/v) 12.5 μl of L-Leucine (1 w/v) 12.5 μl of L-Methionine (1 w/v) 12.5 μl of L-Phenylalanine (1 mM) 12.5 μl of L-Tryptophan (1 w/v) 12.5 μl of L-Tyrosine (1 w/v) 12.5 μl of L-Valine (1 w/v) 900.0 μl of water (100 v/v)
A11	L-Alanine 0.05 w/v - L-Arginine 0.05 w/v - L-Asparagine 0.05 w/v - L-Aspartic acid 0.05 w/v - Glycine 0.05 w/v - L-Glutamic acid 0.05 w/v - L-Glutamine 0.05 w/v - L-Histidine 0.05 w/v - L-Isoleucine 0.05 w/v - L-Leucine 0.05 w/v - L-Lysine 0.05 w/v - L-Methionine 0.05 w/v - L-Phenylalanine 0.05 mM - L-Proline 0.05 w/v - L-Serine 0.05 w/v - L-Threonine 0.05 w/v - L-Tryptophan 0.05 w/v - L-Tyrosine 0.05 w/v - L-Valine 0.05 w/v - 🔍	NA	50.0 μl of Glycine (1 w/v) 50.0 μl of L-Alanine (1 w/v) 50.0 μl of L-Arginine (1 w/v) 50.0 μl of L-Asparagine (1 w/v) 50.0 μl of L-Aspartic acid (1 w/v) 50.0 μl of L-Glutamic acid (1 w/v) 50.0 μl of L-Glutamine (1 w/v) 50.0 μl of L-Histidine (1 w/v) 50.0 μl of L-Isoleucine (1 w/v) 50.0 μl of L-Leucine (1 w/v) 50.0 μl of L-Lysine (1 w/v) 50.0 μl of L-Methionine (1 w/v) 50.0 μl of L-Phenylalanine (1 mM) 50.0 μl of L-Proline (1 w/v) 50.0 μl of L-Serine (1 w/v) 50.0 μl of L-Threonine (1 w/v) 50.0 μl of L-Tryptophan (1 w/v) 50.0 μl of L-Tyrosine (1 w/v) 50.0 μl of L-Valine (1 w/v) 50.0 μl of water (100 v/v)
A12	n-Acetyl-L-Glutamic acid 50 mM - 🔍	NA	500.0 μl of n-Acetyl-L-Glutamic acid (100 mM) 500.0 μl of water (100 v/v)
B1	L-Citrulline 60 mM - 🔍	NA	600.0 μl of L-Citrulline (100 mM) 400.0 μl of water (100 v/v)
B2	L-Homoserine 80 mM - 🔍	NA	800.0 μl of L-Homoserine (100 mM) 200.0 μl of water (100 v/v)
B3	trans-4-Hydroxy-L-proline 80 mM - 🔍	NA	800.0 μl of trans-4-Hydroxy-L-proline (100 mM) 200.0 μl of water (100 v/v)
B4	L-Ornithine monohydrochloride 60 mM - 🔍	NA	600.0 μl of L-Ornithine monohydrochloride (100 mM) 400.0 μl of water (100 v/v)
B5	L-Pyroglutamic acid 80 mM - 🔍	NA	800.0 μl of L-Pyroglutamic acid (100 mM) 200.0 μl of water (100 v/v)
B6	n-Acetyl-D-Glucosamine 50 mM - 🔍	NA	500.0 μl of n-Acetyl-D-Glucosamine (100 mM) 500.0 μl of water (100 v/v)
B7	n-Acetyl-D-Mannosamine 50 mM - 🔍	NA	500.0 μl of n-Acetyl-D-Mannosamine (100 mM) 500.0 μl of water (100 v/v)
B8	D-(+)-Galactosamine hydrochloride 5 mM - 🔍	NA	50.0 μl of D-(+)-Galactosamine hydrochloride (100 mM) 950.0 μl of water (100 v/v)
B9	D-(+)-Glucosamine hydrochloride 50 mM - 🔍	NA	500.0 μl of D-(+)-Glucosamine hydrochloride (100 mM) 500.0 μl of water (100 v/v)
B10	n-Dodecyl-ß-D-maltoside 5 w/v - 🔍	NA	1000.0 μl of n-Dodecyl-ß-D-maltoside (5 w/v)
B11	n-Dodecyl-N,N-dimethylamine-N-oxide 5 w/v - 🔍	NA	1000.0 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (5 w/v)
B12	n-Octyl-ß-D-glucoside 5 w/v - 🔍	NA	1000.0 μl of n-Octyl-ß-D-glucoside (5 w/v)
C1	ANAPOE®-20 10 w/v - 🔍	NA	1000.0 μl of ANAPOE®-20 (10 w/v)
C2	ANAPOE®-80 10 w/v - 🔍	NA	1000.0 μl of ANAPOE®-80 (10 w/v)
C3	Calcium chloride 70 mM - 🔍	NA	700.0 μl of Calcium chloride (100 mM) 300.0 μl of water (100 v/v)
C4	Magnesium chloride 50 mM - 🔍	NA	100.0 μl of Magnesium chloride (500 mM) 900.0 μl of water (100 v/v)
C5	Zinc chloride 70 mM - 🔍	NA	350.0 μl of Zinc chloride (200 mM) 650.0 μl of water (100 v/v)
C6	cis-Aconitic acid 60 mM - 🔍	NA	600.0 μl of cis-Aconitic acid (100 mM) 400.0 μl of water (100 v/v)
C7	4-Aminobenzoic acid 7 mM - 🔍	NA	700.0 μl of 4-Aminobenzoic acid (10 mM) 300.0 μl of water (100 v/v)
C8	Creatine 70 mM - 🔍	NA	700.0 μl of Creatine (100 mM) 300.0 μl of water (100 v/v)
C9	Fumaric acid 9 mM - 🔍	NA	90.0 μl of Fumaric acid (100 mM) 910.0 μl of water (100 v/v)
C10	tri-Sodium Isocitrate 40 mM - 🔍	NA	400.0 μl of tri-Sodium Isocitrate (100 mM) 600.0 μl of water (100 v/v)
C11	di-Sodium a-Ketoglutarate 40 mM - 🔍	NA	400.0 μl of di-Sodium a-Ketoglutarate (100 mM) 600.0 μl of water (100 v/v)
C12	L-(+)-Lactic acid 110 mM - 🔍	NA	550.0 μl of L-(+)-Lactic acid (200 mM) 450.0 μl of water (100 v/v)
D1	DL-Malic acid as additive 80 mM - 🔍	NA	800.0 μl of DL-Malic acid as additive (100 mM) 200.0 μl of water (100 v/v)
D2	Oxaloacetic acid 80 mM - 🔍	NA	800.0 μl of Oxaloacetic acid (100 mM) 200.0 μl of water (100 v/v)
D3	Pyruvic acid 110 mM - 🔍	NA	550.0 μl of Pyruvic acid (200 mM) 450.0 μl of water (100 v/v)
D4	Sodium D-gluconate 50 mM - 🔍	NA	500.0 μl of Sodium D-gluconate (100 mM) 500.0 μl of water (100 v/v)
D5	Succinic acid as additive 90 mM - 🔍	NA	900.0 μl of Succinic acid as additive (100 mM) 100.0 μl of water (100 v/v)
D6	Vanillin 7 mM - 🔍	NA	700.0 μl of Vanillin (10 mM) 300.0 μl of water (100 v/v)
D7	Mellitic acid 30 mM - 🔍	NA	300.0 μl of Mellitic acid (100 mM) 700.0 μl of water (100 v/v)
D8	Glycyl-glycyl-glycine 50 mM - 🔍	NA	166.7 μl of Glycyl-glycyl-glycine (300 mM) 833.3 μl of water (100 v/v)
D9	di-Sodium Creatine phosphate 30 mM - 🔍	NA	300.0 μl of di-Sodium Creatine phosphate (100 mM) 700.0 μl of water (100 v/v)
D10	di-Sodium a-D-Glucose 1-phosphate 30 mM - 🔍	NA	300.0 μl of di-Sodium a-D-Glucose 1-phosphate (100 mM) 700.0 μl of water (100 v/v)
D11	di-Sodium phenyl phosphate 40 mM - 🔍	NA	400.0 μl of di-Sodium phenyl phosphate (100 mM) 600.0 μl of water (100 v/v)
D12	Glycerol 20 v/v - 🔍	NA	200.0 μl of Glycerol (100 v/v) 800.0 μl of water (100 v/v)
E1	Polyethylene glycol 200 20 v/v - 🔍	NA	200.0 μl of Polyethylene glycol 200 (100 v/v) 800.0 μl of water (100 v/v)
E2	1,2-Propanediol 1 v/v - 🔍	NA	10.0 μl of 1,2-Propanediol (100 v/v) 990.0 μl of water (100 v/v)
E3	(+)-Arabinogalactan 1 w/v - 🔍	NA	100.0 μl of (+)-Arabinogalactan (10 w/v) 900.0 μl of water (100 v/v)
E4	Sodium Chondroitin sulfate A 1 w/v - 🔍	NA	100.0 μl of Sodium Chondroitin sulfate A (10 w/v) 900.0 μl of water (100 v/v)
E5	Dextran 1,500 1 w/v - 🔍	NA	100.0 μl of Dextran 1,500 (10 w/v) 900.0 μl of water (100 v/v)
E6	Dextran 6,000 1 w/v - 🔍	NA	100.0 μl of Dextran 6,000 (10 w/v) 900.0 μl of water (100 v/v)
E7	Dextran sulfate sodium salt 4,000 1 w/v - 🔍	NA	100.0 μl of Dextran sulfate sodium salt 4,000 (10 w/v) 900.0 μl of water (100 v/v)
E8	Dextran sulfate sodium salt 8,000 1 w/v - 🔍	NA	100.0 μl of Dextran sulfate sodium salt 8,000 (10 w/v) 900.0 μl of water (100 v/v)
E9	Dextran sulfate sodium salt 15,000 1 w/v - 🔍	NA	100.0 μl of Dextran sulfate sodium salt 15,000 (10 w/v) 900.0 μl of water (100 v/v)
E10	Fructooligosaccharides 1 w/v - 🔍	NA	100.0 μl of Fructooligosaccharides (10 w/v) 900.0 μl of water (100 v/v)
E11	Adenosine 4 mM - 🔍	NA	400.0 μl of Adenosine (10 mM) 600.0 μl of water (100 v/v)
E12	Caffeine 50 mM - 🔍	NA	500.0 μl of Caffeine (100 mM) 500.0 μl of water (100 v/v)
F1	Guanosine 0.4 mM - 🔍	NA	40.0 μl of Guanosine (10 mM) 960.0 μl of water (100 v/v)
F2	Inosine 40 mM - 🔍	NA	400.0 μl of Inosine (100 mM) 600.0 μl of water (100 v/v)
F3	Cytidine 40 mM - 🔍	NA	400.0 μl of Cytidine (100 mM) 600.0 μl of water (100 v/v)
F4	Cytosine 9 mM - 🔍	NA	900.0 μl of Cytosine (10 mM) 100.0 μl of water (100 v/v)
F5	Thymidine 40 mM - 🔍	NA	400.0 μl of Thymidine (100 mM) 600.0 μl of water (100 v/v)
F6	Thymine 8 mM - 🔍	NA	800.0 μl of Thymine (10 mM) 200.0 μl of water (100 v/v)
F7	Uridine 40 mM - 🔍	NA	400.0 μl of Uridine (100 mM) 600.0 μl of water (100 v/v)
F8	Sodium chloride 1 M - 🔍	NA	500.0 μl of Sodium chloride (2 M) 500.0 μl of water (100 v/v)
F9	Non-Detergent Sulfobetaine (NDSB) 195 3 M - 🔍	NA	1000.0 μl of Non-Detergent Sulfobetaine (NDSB) 195 (3 M)
F10	L-(+)-Arabinose 250 mM - 🔍	NA	500.0 μl of L-(+)-Arabinose (500 mM) 500.0 μl of water (100 v/v)
F11	L-(-)-Arabitol 250 mM - 🔍	NA	500.0 μl of L-(-)-Arabitol (500 mM) 500.0 μl of water (100 v/v)
F12	D-(+)-Fucose 250 mM - 🔍	NA	500.0 μl of D-(+)-Fucose (500 mM) 500.0 μl of water (100 v/v)
G1	DL-Glyceraldehyde 110 mM - 🔍	NA	550.0 μl of DL-Glyceraldehyde (200 mM) 450.0 μl of water (100 v/v)
G2	Lactose 30 mM - 🔍	NA	300.0 μl of Lactose (100 mM) 700.0 μl of water (100 v/v)
G3	Mannitol 60 mM - 🔍	NA	600.0 μl of Mannitol (100 mM) 400.0 μl of water (100 v/v)
G4	D-(+)-Mannose 60 mM - 🔍	NA	600.0 μl of D-(+)-Mannose (100 mM) 400.0 μl of water (100 v/v)
G5	D-(-)-Ribose 250 mM - 🔍	NA	500.0 μl of D-(-)-Ribose (500 mM) 500.0 μl of water (100 v/v)
G6	D-(+)-Sucrose 250 mM - 🔍	NA	500.0 μl of D-(+)-Sucrose (500 mM) 500.0 μl of water (100 v/v)
G7	D-(+)-Trehalose 250 mM - 🔍	NA	500.0 μl of D-(+)-Trehalose (500 mM) 500.0 μl of water (100 v/v)
G8	DL-Xylose 250 mM - 🔍	NA	500.0 μl of DL-Xylose (500 mM) 500.0 μl of water (100 v/v)
G9	di-Sodium ß-Glycerophosphate 50 mM - 🔍	NA	500.0 μl of di-Sodium ß-Glycerophosphate (100 mM) 500.0 μl of water (100 v/v)
G10	tri-Sodium D-Fructose 1,6-diphosphate 30 mM - 🔍	NA	300.0 μl of tri-Sodium D-Fructose 1,6-diphosphate (100 mM) 700.0 μl of water (100 v/v)
G11	IPTG 40 mM - 🔍	NA	400.0 μl of IPTG (100 mM) 600.0 μl of water (100 v/v)
G12	L-Ascorbic acid 60 mM - 🔍	NA	600.0 μl of L-Ascorbic acid (100 mM) 400.0 μl of water (100 v/v)
H1	D-Biotin 0.4 mM - 🔍	NA	400.0 μl of D-Biotin (1 mM) 600.0 μl of water (100 v/v)
H2	Nicotinic acid 80 mM - 🔍	NA	800.0 μl of Nicotinic acid (100 mM) 200.0 μl of water (100 v/v)
H3	Nicotinamide 80 mM - 🔍	NA	800.0 μl of Nicotinamide (100 mM) 200.0 μl of water (100 v/v)
H4	DL-Panthenol 50 mM - 🔍	NA	500.0 μl of DL-Panthenol (100 mM) 500.0 μl of water (100 v/v)
H5	Calcium pantothenate 40 mM - 🔍	NA	400.0 μl of Calcium pantothenate (100 mM) 600.0 μl of water (100 v/v)
H6	Pyridoxal hydrochloride 50 mM - 🔍	NA	500.0 μl of Pyridoxal hydrochloride (100 mM) 500.0 μl of water (100 v/v)
H7	Pyridoxamine dihydrochloride 40 mM - 🔍	NA	400.0 μl of Pyridoxamine dihydrochloride (100 mM) 600.0 μl of water (100 v/v)
H8	Pyridoxine 60 mM - 🔍	NA	600.0 μl of Pyridoxine (100 mM) 400.0 μl of water (100 v/v)
H9	Pyridoxal 5'-phosphate 4 mM - 🔍	NA	400.0 μl of Pyridoxal 5'-phosphate (10 mM) 600.0 μl of water (100 v/v)
H10	Thiamine hydrochloride 30 mM - 🔍	NA	300.0 μl of Thiamine hydrochloride (100 mM) 700.0 μl of water (100 v/v)
H11	Thiamine pyrophosphate 20 mM - 🔍	NA	200.0 μl of Thiamine pyrophosphate (100 mM) 800.0 μl of water (100 v/v)
H12	Thiamine monophosphate chloride 20 mM - 🔍	NA	200.0 μl of Thiamine monophosphate chloride (100 mM) 800.0 μl of water (100 v/v)