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Supplemental Information

Screen Description

GRAS Additive

GRAS screens / GRAS Additive
Created 2017-07-18.

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GRAS Additive

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GRAS Additive

    Screen Structure

    GRAS Additive

    Well Count 96
    Number of distinct ingredients 109
    Internal diversity (score mean dist) 1.000

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:40:37.872167 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type GRAS Additive
    Buffer 2
    Complex ingredient 1
    Cryoprotectant 1
    Detergent 7
    Organic (non-volatile) 1
    Polymer 1
    Salt 4

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    GRAS Additive Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Na-Benzoyl-L-arginine ethyl ester hydrochloride 30 mM -
    		NA
    300 μl of Na-Benzoyl-L-arginine ethyl ester hydrochloride (100 mM)
    700 μl of water (100 v/v)
    A2
    • L-Carnosine 40 mM -
    		NA
    400 μl of L-Carnosine (100 mM)
    600 μl of water (100 v/v)
    A3
    • Choline chloride 70 mM -
    		NA
    350 μl of Choline chloride (200 mM)
    650 μl of water (100 v/v)
    A4
    • Histamine dihydrochloride 50 mM -
    		NA
    500 μl of Histamine dihydrochloride (100 mM)
    500 μl of water (100 v/v)
    A5
    • Sarcosine 110 mM -
    		NA
    550 μl of Sarcosine (200 mM)
    450 μl of water (100 v/v)
    A6
    • L-Aspartic acid 0.05 w/v -
    • L-Glutamic acid 0.05 w/v -
    		NA
    50 μl of L-Aspartic acid (1 w/v)
    50 μl of L-Glutamic acid (1 w/v)
    900 μl of water (100 v/v)
    A7
    • Glycine 0.5 w/v -
    • L-Proline 0.5 w/v -
    		NA
    500 μl of Glycine (1 w/v)
    500 μl of L-Proline (1 w/v)
    A8
    • L-Arginine 0.33 w/v -
    • L-Histidine 0.33 w/v -
    • L-Lysine 0.33 w/v -
    		NA
    330 μl of L-Lysine (1 w/v)
    330 μl of L-Arginine (1 w/v)
    330 μl of L-Histidine (1 w/v)
    10 μl of water (100 v/v)
    A9
    • L-Asparagine 0.25 w/v -
    • L-Glutamine 0.25 w/v -
    • L-Serine 0.25 w/v -
    • L-Threonine 0.25 w/v -
    		NA
    250 μl of L-Asparagine (1 w/v)
    250 μl of L-Threonine (1 w/v)
    250 μl of L-Serine (1 w/v)
    250 μl of L-Glutamine (1 w/v)
    A10
    • L-Alanine 0.0125 w/v -
    • L-Isoleucine 0.0125 w/v -
    • L-Leucine 0.0125 w/v -
    • L-Methionine 0.0125 w/v -
    • L-Phenylalanine 0.0125 mM -
    • L-Tyrosine 0.0125 w/v -
    • L-Tryptophan 0.0125 w/v -
    • L-Valine 0.0125 w/v -
    		NA
    12 μl of L-Isoleucine (1 w/v)
    12 μl of L-Alanine (1 w/v)
    12 μl of L-Tyrosine (1 w/v)
    12 μl of L-Phenylalanine (1 mM)
    12 μl of L-Tryptophan (1 w/v)
    12 μl of L-Methionine (1 w/v)
    12 μl of L-Valine (1 w/v)
    12 μl of L-Leucine (1 w/v)
    900 μl of water (100 v/v)
    A11
    • L-Alanine 0.05 w/v -
    • L-Arginine 0.05 w/v -
    • L-Asparagine 0.05 w/v -
    • L-Aspartic acid 0.05 w/v -
    • Glycine 0.05 w/v -
    • L-Glutamic acid 0.05 w/v -
    • L-Glutamine 0.05 w/v -
    • L-Histidine 0.05 w/v -
    • L-Isoleucine 0.05 w/v -
    • L-Leucine 0.05 w/v -
    • L-Lysine 0.05 w/v -
    • L-Methionine 0.05 w/v -
    • L-Phenylalanine 0.05 mM -
    • L-Proline 0.05 w/v -
    • L-Serine 0.05 w/v -
    • L-Threonine 0.05 w/v -
    • L-Tryptophan 0.05 w/v -
    • L-Tyrosine 0.05 w/v -
    • L-Valine 0.05 w/v -
    		NA
    50 μl of Glycine (1 w/v)
    50 μl of L-Isoleucine (1 w/v)
    50 μl of L-Lysine (1 w/v)
    50 μl of L-Asparagine (1 w/v)
    50 μl of L-Aspartic acid (1 w/v)
    50 μl of L-Arginine (1 w/v)
    50 μl of L-Alanine (1 w/v)
    50 μl of L-Threonine (1 w/v)
    50 μl of L-Glutamic acid (1 w/v)
    50 μl of L-Serine (1 w/v)
    50 μl of L-Tyrosine (1 w/v)
    50 μl of L-Phenylalanine (1 mM)
    50 μl of L-Tryptophan (1 w/v)
    50 μl of L-Methionine (1 w/v)
    50 μl of L-Proline (1 w/v)
    50 μl of L-Glutamine (1 w/v)
    50 μl of L-Valine (1 w/v)
    50 μl of L-Histidine (1 w/v)
    50 μl of L-Leucine (1 w/v)
    50 μl of water (100 v/v)
    A12
    • n-Acetyl-L-Glutamic acid 50 mM -
    		NA
    500 μl of n-Acetyl-L-Glutamic acid (100 mM)
    500 μl of water (100 v/v)
    B1
    • L-Citrulline 60 mM -
    		NA
    600 μl of L-Citrulline (100 mM)
    400 μl of water (100 v/v)
    B2
    • L-Homoserine 80 mM -
    		NA
    800 μl of L-Homoserine (100 mM)
    200 μl of water (100 v/v)
    B3
    • trans-4-Hydroxy-L-proline 80 mM -
    		NA
    800 μl of trans-4-Hydroxy-L-proline (100 mM)
    200 μl of water (100 v/v)
    B4
    • L-Ornithine monohydrochloride 60 mM -
    		NA
    600 μl of L-Ornithine monohydrochloride (100 mM)
    400 μl of water (100 v/v)
    B5
    • L-Pyroglutamic acid 80 mM -
    		NA
    800 μl of L-Pyroglutamic acid (100 mM)
    200 μl of water (100 v/v)
    B6
    • n-Acetyl-D-Glucosamine 50 mM -
    		NA
    500 μl of n-Acetyl-D-Glucosamine (100 mM)
    500 μl of water (100 v/v)
    B7
    • n-Acetyl-D-Mannosamine 50 mM -
    		NA
    500 μl of n-Acetyl-D-Mannosamine (100 mM)
    500 μl of water (100 v/v)
    B8
    • D-(+)-Galactosamine hydrochloride 5 mM -
    		NA
    50 μl of D-(+)-Galactosamine hydrochloride (100 mM)
    950 μl of water (100 v/v)
    B9
    • D-(+)-Glucosamine hydrochloride 50 mM -
    		NA
    500 μl of D-(+)-Glucosamine hydrochloride (100 mM)
    500 μl of water (100 v/v)
    B10
    • n-Dodecyl-ß-D-maltoside 5 w/v -
    		NA
    1000 μl of n-Dodecyl-ß-D-maltoside (5 w/v)
    B11
    • n-Dodecyl-N,N-dimethylamine-N-oxide 5 w/v -
    		NA
    1000 μl of n-Dodecyl-N,N-dimethylamine-N-oxide (5 w/v)
    B12
    • n-Octyl-ß-D-glucoside 5 w/v -
    		NA
    1000 μl of n-Octyl-ß-D-glucoside (5 w/v)
    C1
    • ANAPOE®-20 10 w/v -
    		NA
    1000 μl of ANAPOE®-20 (10 w/v)
    C2
    • ANAPOE®-80 10 w/v -
    		NA
    1000 μl of ANAPOE®-80 (10 w/v)
    C3
    • Calcium chloride 70 mM -
    		NA
    700 μl of Calcium chloride (100 mM)
    300 μl of water (100 v/v)
    C4
    • Magnesium chloride 50 mM -
    		NA
    100 μl of Magnesium chloride (500 mM)
    900 μl of water (100 v/v)
    C5
    • Zinc chloride 70 mM -
    		NA
    350 μl of Zinc chloride (200 mM)
    650 μl of water (100 v/v)
    C6
    • cis-Aconitic acid 60 mM -
    		NA
    600 μl of cis-Aconitic acid (100 mM)
    400 μl of water (100 v/v)
    C7
    • 4-Aminobenzoic acid 7 mM -
    		NA
    700 μl of 4-Aminobenzoic acid (10 mM)
    300 μl of water (100 v/v)
    C8
    • Creatine 70 mM -
    		NA
    700 μl of Creatine (100 mM)
    300 μl of water (100 v/v)
    C9
    • Fumaric acid 9 mM -
    		NA
    90 μl of Fumaric acid (100 mM)
    910 μl of water (100 v/v)
    C10
    • tri-Sodium Isocitrate 40 mM -
    		NA
    400 μl of tri-Sodium Isocitrate (100 mM)
    600 μl of water (100 v/v)
    C11
    • di-Sodium a-Ketoglutarate 40 mM -
    		NA
    400 μl of di-Sodium a-Ketoglutarate (100 mM)
    600 μl of water (100 v/v)
    C12
    • L-(+)-Lactic acid 110 mM -
    		NA
    550 μl of L-(+)-Lactic acid (200 mM)
    450 μl of water (100 v/v)
    D1
    • DL-Malic acid as additive 80 mM -
    		NA
    800 μl of DL-Malic acid as additive (100 mM)
    200 μl of water (100 v/v)
    D2
    • Oxaloacetic acid 80 mM -
    		NA
    800 μl of Oxaloacetic acid (100 mM)
    200 μl of water (100 v/v)
    D3
    • Pyruvic acid 110 mM -
    		NA
    550 μl of Pyruvic acid (200 mM)
    450 μl of water (100 v/v)
    D4
    • Sodium D-gluconate 50 mM -
    		NA
    500 μl of Sodium D-gluconate (100 mM)
    500 μl of water (100 v/v)
    D5
    • Succinic acid as additive 90 mM -
    		NA
    900 μl of Succinic acid as additive (100 mM)
    100 μl of water (100 v/v)
    D6
    • Vanillin 7 mM -
    		NA
    700 μl of Vanillin (10 mM)
    300 μl of water (100 v/v)
    D7
    • Mellitic acid 30 mM -
    		NA
    300 μl of Mellitic acid (100 mM)
    700 μl of water (100 v/v)
    D8
    • Glycyl-glycyl-glycine 50 mM -
    		NA
    166 μl of Glycyl-glycyl-glycine (300 mM)
    833 μl of water (100 v/v)
    D9
    • di-Sodium Creatine phosphate 30 mM -
    		NA
    300 μl of di-Sodium Creatine phosphate (100 mM)
    700 μl of water (100 v/v)
    D10
    • di-Sodium a-D-Glucose 1-phosphate 30 mM -
    		NA
    300 μl of di-Sodium a-D-Glucose 1-phosphate (100 mM)
    700 μl of water (100 v/v)
    D11
    • di-Sodium phenyl phosphate 40 mM -
    		NA
    400 μl of di-Sodium phenyl phosphate (100 mM)
    600 μl of water (100 v/v)
    D12
    • Glycerol 20 v/v -
    		NA
    200 μl of Glycerol (100 v/v)
    800 μl of water (100 v/v)
    E1
    • Polyethylene glycol 200 20 v/v -
    		NA
    200 μl of Polyethylene glycol 200 (100 v/v)
    800 μl of water (100 v/v)
    E2
    • 1,2-Propanediol 1 v/v -
    		NA
    10 μl of 1,2-Propanediol (100 v/v)
    990 μl of water (100 v/v)
    E3
    • (+)-Arabinogalactan 1 w/v -
    		NA
    100 μl of (+)-Arabinogalactan (10 w/v)
    900 μl of water (100 v/v)
    E4
    • Sodium Chondroitin sulfate A 1 w/v -
    		NA
    100 μl of Sodium Chondroitin sulfate A (10 w/v)
    900 μl of water (100 v/v)
    E5
    • Dextran 1,500 1 w/v -
    		NA
    100 μl of Dextran 1,500 (10 w/v)
    900 μl of water (100 v/v)
    E6
    • Dextran 6,000 1 w/v -
    		NA
    100 μl of Dextran 6,000 (10 w/v)
    900 μl of water (100 v/v)
    E7
    • Dextran sulfate sodium salt 4,000 1 w/v -
    		NA
    100 μl of Dextran sulfate sodium salt 4,000 (10 w/v)
    900 μl of water (100 v/v)
    E8
    • Dextran sulfate sodium salt 8,000 1 w/v -
    		NA
    100 μl of Dextran sulfate sodium salt 8,000 (10 w/v)
    900 μl of water (100 v/v)
    E9
    • Dextran sulfate sodium salt 15,000 1 w/v -
    		NA
    100 μl of Dextran sulfate sodium salt 15,000 (10 w/v)
    900 μl of water (100 v/v)
    E10
    • Fructooligosaccharides 1 w/v -
    		NA
    100 μl of Fructooligosaccharides (10 w/v)
    900 μl of water (100 v/v)
    E11
    • Adenosine 4 mM -
    		NA
    400 μl of Adenosine (10 mM)
    600 μl of water (100 v/v)
    E12
    • Caffeine 50 mM -
    		NA
    500 μl of Caffeine (100 mM)
    500 μl of water (100 v/v)
    F1
    • Guanosine 0.4 mM -
    		NA
    40 μl of Guanosine (10 mM)
    960 μl of water (100 v/v)
    F2
    • Inosine 40 mM -
    		NA
    400 μl of Inosine (100 mM)
    600 μl of water (100 v/v)
    F3
    • Cytidine 40 mM -
    		NA
    400 μl of Cytidine (100 mM)
    600 μl of water (100 v/v)
    F4
    • Cytosine 9 mM -
    		NA
    900 μl of Cytosine (10 mM)
    100 μl of water (100 v/v)
    F5
    • Thymidine 40 mM -
    		NA
    400 μl of Thymidine (100 mM)
    600 μl of water (100 v/v)
    F6
    • Thymine 8 mM -
    		NA
    800 μl of Thymine (10 mM)
    200 μl of water (100 v/v)
    F7
    • Uridine 40 mM -
    		NA
    400 μl of Uridine (100 mM)
    600 μl of water (100 v/v)
    F8
    • Sodium chloride 1 M -
    		NA
    500 μl of Sodium chloride (2 M)
    500 μl of water (100 v/v)
    F9
    • Non-Detergent Sulfobetaine (NDSB) 195 3 M -
    		NA
    1000 μl of Non-Detergent Sulfobetaine (NDSB) 195 (3 M)
    F10
    • L-(+)-Arabinose 250 mM -
    		NA
    500 μl of L-(+)-Arabinose (500 mM)
    500 μl of water (100 v/v)
    F11
    • L-(-)-Arabitol 250 mM -
    		NA
    500 μl of L-(-)-Arabitol (500 mM)
    500 μl of water (100 v/v)
    F12
    • D-(+)-Fucose 250 mM -
    		NA
    500 μl of D-(+)-Fucose (500 mM)
    500 μl of water (100 v/v)
    G1
    • DL-Glyceraldehyde 110 mM -
    		NA
    550 μl of DL-Glyceraldehyde (200 mM)
    450 μl of water (100 v/v)
    G2
    • Lactose 30 mM -
    		NA
    300 μl of Lactose (100 mM)
    700 μl of water (100 v/v)
    G3
    • Mannitol 60 mM -
    		NA
    600 μl of Mannitol (100 mM)
    400 μl of water (100 v/v)
    G4
    • D-(+)-Mannose 60 mM -
    		NA
    600 μl of D-(+)-Mannose (100 mM)
    400 μl of water (100 v/v)
    G5
    • D-(-)-Ribose 250 mM -
    		NA
    500 μl of D-(-)-Ribose (500 mM)
    500 μl of water (100 v/v)
    G6
    • D-(+)-Sucrose 250 mM -
    		NA
    500 μl of D-(+)-Sucrose (500 mM)
    500 μl of water (100 v/v)
    G7
    • D-(+)-Trehalose 250 mM -
    		NA
    500 μl of D-(+)-Trehalose (500 mM)
    500 μl of water (100 v/v)
    G8
    • DL-Xylose 250 mM -
    		NA
    500 μl of DL-Xylose (500 mM)
    500 μl of water (100 v/v)
    G9
    • di-Sodium ß-Glycerophosphate 50 mM -
    		NA
    500 μl of di-Sodium ß-Glycerophosphate (100 mM)
    500 μl of water (100 v/v)
    G10
    • tri-Sodium D-Fructose 1,6-diphosphate 30 mM -
    		NA
    300 μl of tri-Sodium D-Fructose 1,6-diphosphate (100 mM)
    700 μl of water (100 v/v)
    G11
    • IPTG 40 mM -
    		NA
    400 μl of IPTG (100 mM)
    600 μl of water (100 v/v)
    G12
    • L-Ascorbic acid 60 mM -
    		NA
    600 μl of L-Ascorbic acid (100 mM)
    400 μl of water (100 v/v)
    H1
    • D-Biotin 0.4 mM -
    		NA
    400 μl of D-Biotin (1 mM)
    600 μl of water (100 v/v)
    H2
    • Nicotinic acid 80 mM -
    		NA
    800 μl of Nicotinic acid (100 mM)
    200 μl of water (100 v/v)
    H3
    • Nicotinamide 80 mM -
    		NA
    800 μl of Nicotinamide (100 mM)
    200 μl of water (100 v/v)
    H4
    • DL-Panthenol 50 mM -
    		NA
    500 μl of DL-Panthenol (100 mM)
    500 μl of water (100 v/v)
    H5
    • Calcium pantothenate 40 mM -
    		NA
    400 μl of Calcium pantothenate (100 mM)
    600 μl of water (100 v/v)
    H6
    • Pyridoxal hydrochloride 50 mM -
    		NA
    500 μl of Pyridoxal hydrochloride (100 mM)
    500 μl of water (100 v/v)
    H7
    • Pyridoxamine dihydrochloride 40 mM -
    		NA
    400 μl of Pyridoxamine dihydrochloride (100 mM)
    600 μl of water (100 v/v)
    H8
    • Pyridoxine 60 mM -
    		NA
    600 μl of Pyridoxine (100 mM)
    400 μl of water (100 v/v)
    H9
    • Pyridoxal 5'-phosphate 4 mM -
    		NA
    400 μl of Pyridoxal 5'-phosphate (10 mM)
    600 μl of water (100 v/v)
    H10
    • Thiamine hydrochloride 30 mM -
    		NA
    300 μl of Thiamine hydrochloride (100 mM)
    700 μl of water (100 v/v)
    H11
    • Thiamine pyrophosphate 20 mM -
    		NA
    200 μl of Thiamine pyrophosphate (100 mM)
    800 μl of water (100 v/v)
    H12
    • Thiamine monophosphate chloride 20 mM -
    		NA
    200 μl of Thiamine monophosphate chloride (100 mM)
    800 μl of water (100 v/v)