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Supplemental Information

Screen Description

MIDASplus

Created 2018-03-08.

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MIDASplus

    Screen Structure

    MIDASplus

    Well Count 96
    Number of distinct ingredients 61
    Internal diversity (score mean dist) 0.953

    Screen Composition

    2025-06-15T02:23:13.280214 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MIDASplus
    Buffer 90
    Organic (non-volatile) 14
    Organic (volatile) 10
    Polymer 72
    Salt 48

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MIDASplus Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polypropylene glycol 400 50 v/v -
    • Dimethyl sulfoxide 5 v/v -
    • HEPES 0.1 M pH 6
    pH 6
    500 μl of Polypropylene glycol 400 (100 v/v)
    11 μl of HEPES pH 7 (1 M)
    166 μl of Dimethyl sulfoxide (30 v/v)
    88 μl of HEPES pH 5 (1 M)
    233 μl of water (100 v/v)
    A2
    • Polyvinylpyrrolidone K15 12 w/v -
    • MES 0.1 M pH 5.5
    pH 5.5
    240 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of MES pH 5.5 (1 M)
    660 μl of water (100 v/v)
    A3
    • Poly(acrylic acid sodium salt) 2,100 45 w/v -
    • HEPES 0.1 M pH 6.5
    pH 6.5
    900 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    37 μl of HEPES pH 7 (1 M)
    63 μl of HEPES pH 5 (1 M)
    A4
    • Poly(acrylic acid-co-maleic acid) 14 v/v -
    NA
    280 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
    720 μl of water (100 v/v)
    A5
    • Poly(acrylic acid sodium salt) 2,100 12.5 w/v -
    • Ammonium di-hydrogen phosphate 0.5 M -
    NA
    250 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    200 μl of Ammonium di-hydrogen phosphate (2.5 M)
    550 μl of water (100 v/v)
    A6
    • Poly(acrylic acid sodium salt) 2,100 19 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    380 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    520 μl of water (100 v/v)
    A7
    • Polypropylene glycol 400 10 v/v -
    NA
    100 μl of Polypropylene glycol 400 (100 v/v)
    900 μl of water (100 v/v)
    A8
    • Poly(acrylic acid sodium salt) 2,100 5 w/v -
    NA
    100 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    900 μl of water (100 v/v)
    A9
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
    • MES 0.1 M pH 6
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    500 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    300 μl of water (100 v/v)
    A10
    • Polyvinylpyrrolidone K15 24 w/v -
    • Sodium sulfate 0.1 M -
    NA
    480 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of Sodium sulfate (1 M)
    420 μl of water (100 v/v)
    A11
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 v/v -
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 6.5
    pH 6.5
    37 μl of HEPES pH 7 (1 M)
    700 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    100 μl of Calcium chloride (2 M)
    63 μl of HEPES pH 5 (1 M)
    100 μl of water (100 v/v)
    A12
    • Polypropylene glycol 400 35 v/v -
    • Potassium Sodium phosphate 0.1 M pH 7
    pH 7
    350 μl of Polypropylene glycol 400 (100 v/v)
    0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    33 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    616 μl of water (100 v/v)
    B1
    • Sokalan® CP45 20 v/v -
    • Sodium formate 0.1 M -
    NA
    25 μl of Sodium formate (4 M)
    400 μl of Sokalan® CP45 (50 v/v)
    575 μl of water (100 v/v)
    B2
    • Pentaerythritol Propoxylate (5/4 PO/OH) 15 v/v -
    • Sodium thiocyanate 0.2 M -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    300 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    25 μl of Sodium thiocyanate (8 M)
    575 μl of water (100 v/v)
    B3
    • Sodium chloride 0.1 M -
    • HEPES 0.1 M pH 7
    • Sokalan® PA25 25 w/v -
    pH 7
    500 μl of Sokalan® PA25 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    100 μl of Sodium chloride (1 M)
    300 μl of water (100 v/v)
    B4
    • Pentaerythritol Propoxylate (5/4 PO/OH) 45 v/v -
    • Sodium chloride 0.2 M -
    • MES 0.1 M pH 6
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    40 μl of Sodium chloride (5 M)
    450 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    310 μl of water (100 v/v)
    B5
    • Polyvinyl alcohol Type II 8 w/v -
    • 1-Propanol 10 v/v -
    • HEPES 0.1 M pH 7
    pH 7
    250 μl of 1-Propanol (40 v/v)
    100 μl of HEPES pH 7 (1 M)
    160 μl of Polyvinyl alcohol Type II (50 w/v)
    490 μl of water (100 v/v)
    B6
    • Polyvinylpyrrolidone K15 30 w/v -
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 7
    pH 7
    600 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    50 μl of Lithium sulfate (2 M)
    250 μl of water (100 v/v)
    B7
    • Polypropylene glycol 400 40 v/v -
    • Imidazole 0.2 M pH 7
    pH 7
    400 μl of Polypropylene glycol 400 (100 v/v)
    94 μl of Imidazole pH 8 (1 M)
    105 μl of Imidazole pH 6.2 (1 M)
    400 μl of water (100 v/v)
    B8
    • Poly(acrylic acid-co-maleic acid) 8 v/v -
    • HEPES 0.1 M pH 7.5
    • Pentaerythritol ethoxylate (3/4 EO/OH) 3 v/v -
    • Lithium sulfate 0.06 M -
    pH 7.5
    30 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    160 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
    30 μl of Lithium sulfate (2 M)
    680 μl of water (100 v/v)
    B9
    • Sokalan® PA25 20 w/v -
    • di-Sodium tartrate 0.1 M -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    400 μl of Sokalan® PA25 (50 w/v)
    66 μl of di-Sodium tartrate (1.5 M)
    433 μl of water (100 v/v)
    B10
    • Jeffamine® M-600 30 v/v -
    • Dimethyl sulfoxide 10 v/v -
    NA
    333 μl of Dimethyl sulfoxide (30 v/v)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    66 μl of water (100 v/v)
    B11
    • Polypropylene glycol 400 20 v/v -
    • 1-Propanol 10 v/v -
    NA
    250 μl of 1-Propanol (40 v/v)
    200 μl of Polypropylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    B12
    • Poly(acrylic acid-co-maleic acid) 28 v/v -
    • HEPES 0.1 M pH 6.5
    pH 6.5
    37 μl of HEPES pH 7 (1 M)
    63 μl of HEPES pH 5 (1 M)
    560 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
    340 μl of water (100 v/v)
    C1
    • Ethanol 10 v/v -
    • Jeffamine® ED-2003 15 w/v -
    NA
    100 μl of Ethanol (100 v/v)
    300 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
    600 μl of water (100 v/v)
    C2
    • MES 0.1 M pH 6
    • Sodium chloride 0.2 M -
    • Jeffamine® ED-2003 30 w/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    600 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
    40 μl of Sodium chloride (5 M)
    160 μl of water (100 v/v)
    C3
    • MES 0.1 M pH 5.5
    • di-Sodium malonate 0.1 M -
    • Sokalan® CP45 25 v/v -
    pH 5.5
    33 μl of di-Sodium malonate pH 7 (3 M)
    500 μl of Sokalan® CP45 (50 v/v)
    100 μl of MES pH 5.5 (1 M)
    366 μl of water (100 v/v)
    C4
    • MES 0.1 M pH 6
    • Sodium chloride 0.2 M -
    • Pentaerythritol Propoxylate (5/4 PO/OH) 15 v/v -
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    40 μl of Sodium chloride (5 M)
    150 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    610 μl of water (100 v/v)
    C5
    • Magnesium chloride 0.2 M -
    • Pentaerythritol ethoxylate (3/4 EO/OH) 35 v/v -
    NA
    350 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
    100 μl of Magnesium chloride (2 M)
    550 μl of water (100 v/v)
    C6
    • Ethanol 15 v/v -
    • Pentaerythritol Propoxylate (5/4 PO/OH) 40 v/v -
    NA
    150 μl of Ethanol (100 v/v)
    400 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    450 μl of water (100 v/v)
    C7
    • tris chloride BCC 0.1 M pH 8
    • Pentaerythritol Propoxylate (5/4 PO/OH) 50 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    400 μl of water (100 v/v)
    C8
    • tris chloride BCC 0.1 M pH 8
    • Sodium chloride 0.2 M -
    • Polyethylene glycol 4,000 10 w/v -
    • Polyvinylpyrrolidone K15 12.5 w/v -
    pH 8
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    250 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    40 μl of Sodium chloride (5 M)
    410 μl of water (100 v/v)
    C9
    • Dimethyl sulfoxide 10 v/v -
    • Sodium chloride 0.1 M -
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
    NA
    333 μl of Dimethyl sulfoxide (30 v/v)
    20 μl of Sodium chloride (5 M)
    500 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    146 μl of water (100 v/v)
    C10
    • Poly(acrylic acid sodium salt) 2,100 35 w/v -
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    700 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    142 μl of water (100 v/v)
    C11
    • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
    • Magnesium formate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    100 μl of Magnesium formate (1 M)
    200 μl of water (100 v/v)
    C12
    • Poly(acrylic acid-co-maleic acid) 24 v/v -
    • Potassium acetate 0.2 M -
    NA
    40 μl of Potassium acetate (5 M)
    480 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
    480 μl of water (100 v/v)
    D1
    • Polypropylene glycol 400 60 v/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    600 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    300 μl of water (100 v/v)
    D2
    • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
    • Polyvinylpyrrolidone K15 6 w/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    120 μl of Polyvinylpyrrolidone K15 (50 w/v)
    600 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    180 μl of water (100 v/v)
    D3
    • Polypropylene glycol 400 45 v/v -
    • Ethanol 10 v/v -
    NA
    450 μl of Polypropylene glycol 400 (100 v/v)
    100 μl of Ethanol (100 v/v)
    450 μl of water (100 v/v)
    D4
    • Pentaerythritol ethoxylate (3/4 EO/OH) 10 v/v -
    • 1-Butanol 10 v/v -
    NA
    100 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
    100 μl of 1-Butanol (100 v/v)
    800 μl of water (100 v/v)
    D5
    • PPGBA 2000 6 v/v -
    • Poly(acrylic acid sodium salt) 2,100 12.5 w/v -
    • HEPES 0.1 M pH 7
    pH 7
    250 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    120 μl of PPGBA 2000 (50 v/v)
    530 μl of water (100 v/v)
    D6
    • HEPES 0.1 M pH 6.5
    • Polyvinylpyrrolidone K15 6 w/v -
    pH 6.5
    120 μl of Polyvinylpyrrolidone K15 (50 w/v)
    37 μl of HEPES pH 7 (1 M)
    63 μl of HEPES pH 5 (1 M)
    780 μl of water (100 v/v)
    D7
    • HEPES 0.1 M pH 6.5
    • Jeffamine® ED-2003 20 v/v -
    pH 6.5
    400 μl of Jeffamine® ED-2003 pH 7 (50 v/v)
    37 μl of HEPES pH 7 (1 M)
    63 μl of HEPES pH 5 (1 M)
    500 μl of water (100 v/v)
    D8
    • Glycerol Ethoxylate 20 v/v -
    • Tetrahydrofuran 10 v/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Tetrahydrofuran (50 v/v)
    400 μl of Glycerol Ethoxylate (50 v/v)
    300 μl of water (100 v/v)
    D9
    • PPGBA 2000 25 v/v -
    • Imidazole 0.2 M pH 7
    pH 7
    94 μl of Imidazole pH 8 (1 M)
    500 μl of PPGBA 2000 (50 v/v)
    105 μl of Imidazole pH 6.2 (1 M)
    300 μl of water (100 v/v)
    D10
    • PPGBA 230 30 v/v -
    • Potassium chloride 0.2 M -
    • HEPES 0.1 M pH 6.5
    pH 6.5
    37 μl of HEPES pH 7 (1 M)
    50 μl of Potassium chloride (4 M)
    600 μl of PPGBA 230 (50 v/v)
    63 μl of HEPES pH 5 (1 M)
    250 μl of water (100 v/v)
    D11
    • Polypropylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    NA
    300 μl of Polypropylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    D12
    • PPGBA 400 20 v/v -
    • 1-Propanol 15 v/v -
    NA
    375 μl of 1-Propanol (40 v/v)
    400 μl of PPGBA 400 (50 v/v)
    225 μl of water (100 v/v)
    E1
    • PPGBA 400 15 v/v -
    • tri-Lithium citrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    66 μl of tri-Lithium citrate (1.5 M)
    300 μl of PPGBA 400 (50 v/v)
    533 μl of water (100 v/v)
    E2
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
    • Potassium acetate 0.2 M -
    NA
    40 μl of Potassium acetate (5 M)
    350 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    610 μl of water (100 v/v)
    E3
    • Glycine 0.1 M pH 9.5
    • Potassium chloride 0.2 M -
    • Pentaerythritol ethoxylate (15/4 EO/OH) 20 v/v -
    pH 9.5
    41 μl of Glycine pH 10 (1 M)
    50 μl of Potassium chloride (4 M)
    400 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    58 μl of Glycine pH 9 (1 M)
    450 μl of water (100 v/v)
    E4
    • Pentaerythritol Propoxylate (5/4 PO/OH) 40 v/v -
    • Sodium thiocyanate 0.2 M -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    400 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
    25 μl of Sodium thiocyanate (8 M)
    475 μl of water (100 v/v)
    E5
    • Sokalan® CP45 25 v/v -
    NA
    500 μl of Sokalan® CP45 (50 v/v)
    500 μl of water (100 v/v)
    E6
    • Pentaerythritol ethoxylate (15/4 EO/OH) 15 v/v -
    • Potassium acetate 0.2 M -
    • MES 0.1 M pH 6
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    300 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    40 μl of Potassium acetate (5 M)
    460 μl of water (100 v/v)
    E7
    • Poly(acrylic acid sodium salt) 2,100 30 w/v -
    • di-Sodium malonate 0.1 M -
    • HEPES 0.1 M pH 7
    pH 7
    600 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    33 μl of di-Sodium malonate pH 7 (3 M)
    266 μl of water (100 v/v)
    E8
    • PPGBA 230 10 v/v -
    • Jeffamine® M-600 10 v/v -
    • Ethanol 10 v/v -
    NA
    100 μl of Ethanol (100 v/v)
    200 μl of PPGBA 230 (50 v/v)
    100 μl of Jeffamine® M-600 (100 v/v)
    600 μl of water (100 v/v)
    E9
    • Jeffamine® ED-2003 25 w/v -
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    500 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Lithium sulfate (2 M)
    350 μl of water (100 v/v)
    E10
    • Sokalan® PA25 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    400 μl of Sokalan® PA25 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of water (100 v/v)
    E11
    • Poly(acrylic acid sodium salt) 2,100 25 w/v -
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 6.5
    pH 6.5
    500 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    82 μl of HEPES pH 5 (1 M)
    17 μl of HEPES pH 7.5 (1 M)
    50 μl of Lithium sulfate (2 M)
    350 μl of water (100 v/v)
    E12
    • Poly(acrylic acid sodium salt) 2,100 15 w/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    300 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    50 μl of Magnesium chloride (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    550 μl of water (100 v/v)
    F1
    • HEPES 0.1 M pH 6.5
    • PPGBA 2000 40 v/v -
    pH 6.5
    800 μl of PPGBA 2000 (50 v/v)
    82 μl of HEPES pH 5 (1 M)
    17 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    F2
    • Poly(acrylic acid sodium salt) 2,100 10 w/v -
    • Sodium chloride 0.5 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    200 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    100 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    F3
    • PPGBA 230 10 v/v -
    • Potassium Sodium phosphate 0.1 M pH 7
    • PPGBA 400 15 v/v -
    pH 7
    200 μl of PPGBA 230 (50 v/v)
    0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    300 μl of PPGBA 400 (50 v/v)
    33 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    466 μl of water (100 v/v)
    F4
    • Poly(acrylic acid sodium salt) 2,100 20 w/v -
    • Sodium chloride 0.2 M -
    • BICINE 0.1 M pH 9
    pH 9
    400 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
    9 μl of BICINE pH 7.4 (1 M)
    90 μl of BICINE pH 9.3 (1 M)
    40 μl of Sodium chloride (5 M)
    460 μl of water (100 v/v)
    F5
    • PPGBA 2000 20 v/v -
    • di-Sodium malonate 0.2 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    66 μl of di-Sodium malonate pH 7 (3 M)
    400 μl of PPGBA 2000 (50 v/v)
    100 μl of MES pH 5.5 (1 M)
    433 μl of water (100 v/v)
    F6
    • Sokalan® CP45 25 v/v -
    • Cesium chloride 0.1 M -
    NA
    500 μl of Sokalan® CP45 (50 v/v)
    100 μl of Cesium chloride (1 M)
    400 μl of water (100 v/v)
    F7
    • Sokalan® PA25 25 w/v -
    NA
    500 μl of Sokalan® PA25 (50 w/v)
    500 μl of water (100 v/v)
    F8
    • PPGBA 400 30 v/v -
    • Lithium nitrate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    600 μl of PPGBA 400 (50 v/v)
    25 μl of Lithium nitrate (8 M)
    275 μl of water (100 v/v)
    F9
    • Poly(acrylic acid sodium salt) 5,100 20 w/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    500 μl of water (100 v/v)
    F10
    • Ethylene imine polymer, branched 28 v/v -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    560 μl of Ethylene imine polymer, branched (50 v/v)
    340 μl of water (100 v/v)
    F11
    • Sokalan® CP7 20 v/v -
    • Ammonium formate 0.1 M -
    • HEPES 0.1 M pH 7
    pH 7
    10 μl of Ammonium formate (10 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Sokalan® CP7 (50 v/v)
    490 μl of water (100 v/v)
    F12
    • Sokalan® HP56 20 w/v -
    • Sodium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    200 μl of Sodium sulfate (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Sokalan® HP56 (50 w/v)
    300 μl of water (100 v/v)
    G1
    • Sokalan® CP7 25 v/v -
    • Potassium chloride 0.1 M -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    500 μl of Sokalan® CP7 (50 v/v)
    375 μl of water (100 v/v)
    G2
    • Sokalan® CP5 20 v/v -
    • Ammonium formate 0.3 M -
    • HEPES 0.1 M pH 7
    pH 7
    30 μl of Ammonium formate (10 M)
    400 μl of Sokalan® CP5 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    470 μl of water (100 v/v)
    G3
    • Glycerol Ethoxylate 40 v/v -
    NA
    800 μl of Glycerol Ethoxylate (50 v/v)
    200 μl of water (100 v/v)
    G4
    • tris chloride BCC 0.1 M pH 8.5
    • Glycerol Ethoxylate 30 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Glycerol Ethoxylate (50 v/v)
    300 μl of water (100 v/v)
    G5
    • Polypropylene glycol 400 55 v/v -
    NA
    550 μl of Polypropylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    G6
    • Glycerol Ethoxylate 35 v/v -
    • tri-Lithium citrate 0.2 M -
    NA
    133 μl of tri-Lithium citrate (1.5 M)
    700 μl of Glycerol Ethoxylate (50 v/v)
    166 μl of water (100 v/v)
    G7
    • Glycerol Ethoxylate 30 v/v -
    • Ammonium acetate 0.2 M -
    • MES 0.1 M pH 6.5
    pH 6.5
    151 μl of MES pH 7.1 (0.5 M)
    48 μl of MES pH 5.2 (0.5 M)
    66 μl of Ammonium acetate (3 M)
    600 μl of Glycerol Ethoxylate (50 v/v)
    133 μl of water (100 v/v)
    G8
    • Sokalan® CP42 20 v/v -
    • Methanol 5 v/v -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of Methanol (50 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    400 μl of Sokalan® CP42 (50 v/v)
    400 μl of water (100 v/v)
    G9
    • Sokalan® CP42 25 v/v -
    • Tetrahydrofuran 10 v/v -
    • tris chloride BCC 0.1 M pH 7
    pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of Sokalan® CP42 (50 v/v)
    200 μl of Tetrahydrofuran (50 v/v)
    200 μl of water (100 v/v)
    G10
    • Sokalan® CP42 20 v/v -
    • Lithium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 6
    pH 6
    63 μl of BIS-TRIS pH 5.5 (1 M)
    36 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Sokalan® CP42 (50 v/v)
    20 μl of Lithium acetate (5 M)
    480 μl of water (100 v/v)
    G11
    • PPGBA 400 20 v/v -
    • BIS-TRIS 0.1 M pH 5.5
    • Sodium chloride 0.1 M -
    pH 5.5
    100 μl of Sodium chloride (1 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    400 μl of PPGBA 400 (50 v/v)
    400 μl of water (100 v/v)
    G12
    • Sokalan® CP5 15 v/v -
    • BIS-TRIS 0.1 M pH 6
    pH 6
    300 μl of Sokalan® CP5 (50 v/v)
    63 μl of BIS-TRIS pH 5.5 (1 M)
    36 μl of BIS-TRIS pH 6.5 (1 M)
    600 μl of water (100 v/v)
    H1
    • Sokalan® CP42 25 v/v -
    • BIS-TRIS 0.1 M pH 6
    pH 6
    63 μl of BIS-TRIS pH 5.5 (1 M)
    36 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Sokalan® CP42 (50 v/v)
    400 μl of water (100 v/v)
    H2
    • PPGBA 400 25 v/v -
    • Ammonium formate 0.2 M -
    NA
    80 μl of Ammonium formate (2.5 M)
    500 μl of PPGBA 400 (50 v/v)
    420 μl of water (100 v/v)
    H3
    • Glycerol Ethoxylate 20 v/v -
    • Ethylene imine polymer 3 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    60 μl of Ethylene imine polymer (50 v/v)
    400 μl of Glycerol Ethoxylate (50 v/v)
    440 μl of water (100 v/v)
    H4
    • Glycerol Ethoxylate 25 v/v -
    • Ammonium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    40 μl of Ammonium chloride (5 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Glycerol Ethoxylate (50 v/v)
    360 μl of water (100 v/v)
    H5
    • Sokalan® CP42 10 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    200 μl of Sokalan® CP42 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    700 μl of water (100 v/v)
    H6
    • Poly(acrylic acid sodium salt) 5,100 30 w/v -
    • Ethanol 10 v/v -
    • MES 0.1 M pH 6
    pH 6
    83 μl of MES pH 7.1 (0.5 M)
    117 μl of MES pH 5.2 (0.5 M)
    100 μl of Ethanol (100 v/v)
    600 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    100 μl of water (100 v/v)
    H7
    • Sokalan® CP42 15 v/v -
    • tri-Potassium citrate 0.2 M -
    NA
    300 μl of Sokalan® CP42 (50 v/v)
    80 μl of tri-Potassium citrate (2.5 M)
    620 μl of water (100 v/v)
    H8
    • Sokalan® CP42 30 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    600 μl of Sokalan® CP42 (50 v/v)
    300 μl of water (100 v/v)
    H9
    • Sokalan® HP56 25 w/v -
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7
    pH 7
    100 μl of HEPES pH 7 (1 M)
    500 μl of Sokalan® HP56 (50 w/v)
    66 μl of Ammonium acetate (3 M)
    333 μl of water (100 v/v)
    H10
    • Sokalan® CP5 25 v/v -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    500 μl of Sokalan® CP5 (50 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    H11
    • Polyvinylpyrrolidone K15 10 w/v -
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium formate 0.2 M -
    NA
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Polyvinylpyrrolidone K15 (50 w/v)
    20 μl of Ammonium formate (10 M)
    380 μl of water (100 v/v)
    H12
    • Polyvinylpyrrolidone K15 15 w/v -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 5,000 25 w/v -
    pH 8
    300 μl of Polyvinylpyrrolidone K15 (50 w/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of water (100 v/v)