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Supplemental Information

Screen Description

MIDASplus

Created 2018-03-08.

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Barcodes

MIDASplus

No barcodes found.

Screen Structure

MIDASplus

Well Count 96
Number of distinct ingredients 61
Internal diversity (score mean dist) 0.953

Screen Composition

2025-08-16T06:25:37.996398 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MIDASplus
Buffer 90
Organic (non-volatile) 14
Organic (volatile) 10
Polymer 72
Salt 48

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MIDASplus Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polypropylene glycol 400 50 v/v -
  • Dimethyl sulfoxide 5 v/v -
  • HEPES 0.1 M pH 6
pH 6
166.7 μl of Dimethyl sulfoxide (30 v/v)
88.4 μl of HEPES pH 5 (1 M)
11.6 μl of HEPES pH 7 (1 M)
500.0 μl of Polypropylene glycol 400 (100 v/v)
233.3 μl of water (100 v/v)
A2
  • Polyvinylpyrrolidone K15 12 w/v -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
240.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
660.0 μl of water (100 v/v)
A3
  • Poly(acrylic acid sodium salt) 2,100 45 w/v -
  • HEPES 0.1 M pH 6.5
pH 6.5
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
900.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
A4
  • Poly(acrylic acid-co-maleic acid) 14 v/v -
NA
280.0 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
720.0 μl of water (100 v/v)
A5
  • Poly(acrylic acid sodium salt) 2,100 12.5 w/v -
  • Ammonium di-hydrogen phosphate 0.5 M -
NA
200.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
250.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
550.0 μl of water (100 v/v)
A6
  • Poly(acrylic acid sodium salt) 2,100 19 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
380.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
A7
  • Polypropylene glycol 400 10 v/v -
NA
100.0 μl of Polypropylene glycol 400 (100 v/v)
900.0 μl of water (100 v/v)
A8
  • Poly(acrylic acid sodium salt) 2,100 5 w/v -
NA
100.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
900.0 μl of water (100 v/v)
A9
  • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
500.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
300.0 μl of water (100 v/v)
A10
  • Polyvinylpyrrolidone K15 24 w/v -
  • Sodium sulfate 0.1 M -
NA
480.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of Sodium sulfate (1 M)
420.0 μl of water (100 v/v)
A11
  • Pentaerythritol ethoxylate (15/4 EO/OH) 35 v/v -
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 6.5
pH 6.5
100.0 μl of Calcium chloride (2 M)
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
700.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
100.0 μl of water (100 v/v)
A12
  • Polypropylene glycol 400 35 v/v -
  • Potassium Sodium phosphate 0.1 M pH 7
pH 7
350.0 μl of Polypropylene glycol 400 (100 v/v)
3.0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
30.3 μl of Potassium Sodium phosphate pH 7.5 (3 M)
616.7 μl of water (100 v/v)
B1
  • Sokalan® CP45 20 v/v -
  • Sodium formate 0.1 M -
NA
25.0 μl of Sodium formate (4 M)
400.0 μl of Sokalan® CP45 (50 v/v)
575.0 μl of water (100 v/v)
B2
  • Pentaerythritol Propoxylate (5/4 PO/OH) 15 v/v -
  • Sodium thiocyanate 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
300.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
25.0 μl of Sodium thiocyanate (8 M)
575.0 μl of water (100 v/v)
B3
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7
  • Sokalan® PA25 25 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
100.0 μl of Sodium chloride (1 M)
500.0 μl of Sokalan® PA25 (50 w/v)
300.0 μl of water (100 v/v)
B4
  • Pentaerythritol Propoxylate (5/4 PO/OH) 45 v/v -
  • Sodium chloride 0.2 M -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
450.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
40.0 μl of Sodium chloride (5 M)
310.0 μl of water (100 v/v)
B5
  • Polyvinyl alcohol Type II 8 w/v -
  • 1-Propanol 10 v/v -
  • HEPES 0.1 M pH 7
pH 7
250.0 μl of 1-Propanol (40 v/v)
100.0 μl of HEPES pH 7 (1 M)
160.0 μl of Polyvinyl alcohol Type II (50 w/v)
490.0 μl of water (100 v/v)
B6
  • Polyvinylpyrrolidone K15 30 w/v -
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
50.0 μl of Lithium sulfate (2 M)
600.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
250.0 μl of water (100 v/v)
B7
  • Polypropylene glycol 400 40 v/v -
  • Imidazole 0.2 M pH 7
pH 7
105.9 μl of Imidazole pH 6.2 (1 M)
94.1 μl of Imidazole pH 8 (1 M)
400.0 μl of Polypropylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
B8
  • Poly(acrylic acid-co-maleic acid) 8 v/v -
  • HEPES 0.1 M pH 7.5
  • Pentaerythritol ethoxylate (3/4 EO/OH) 3 v/v -
  • Lithium sulfate 0.06 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
30.0 μl of Lithium sulfate (2 M)
30.0 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
160.0 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
680.0 μl of water (100 v/v)
B9
  • Sokalan® PA25 20 w/v -
  • di-Sodium tartrate 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Sokalan® PA25 (50 w/v)
66.7 μl of di-Sodium tartrate (1.5 M)
433.3 μl of water (100 v/v)
B10
  • Jeffamine® M-600 30 v/v -
  • Dimethyl sulfoxide 10 v/v -
NA
333.3 μl of Dimethyl sulfoxide (30 v/v)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
66.7 μl of water (100 v/v)
B11
  • Polypropylene glycol 400 20 v/v -
  • 1-Propanol 10 v/v -
NA
250.0 μl of 1-Propanol (40 v/v)
200.0 μl of Polypropylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
B12
  • Poly(acrylic acid-co-maleic acid) 28 v/v -
  • HEPES 0.1 M pH 6.5
pH 6.5
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
560.0 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
340.0 μl of water (100 v/v)
C1
  • Ethanol 10 v/v -
  • Jeffamine® ED-2003 15 w/v -
NA
100.0 μl of Ethanol (100 v/v)
300.0 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
600.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 6
  • Sodium chloride 0.2 M -
  • Jeffamine® ED-2003 30 w/v -
pH 6
600.0 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
40.0 μl of Sodium chloride (5 M)
160.0 μl of water (100 v/v)
C3
  • MES 0.1 M pH 5.5
  • di-Sodium malonate 0.1 M -
  • Sokalan® CP45 25 v/v -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
500.0 μl of Sokalan® CP45 (50 v/v)
33.3 μl of di-Sodium malonate pH 7 (3 M)
366.7 μl of water (100 v/v)
C4
  • MES 0.1 M pH 6
  • Sodium chloride 0.2 M -
  • Pentaerythritol Propoxylate (5/4 PO/OH) 15 v/v -
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
150.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
40.0 μl of Sodium chloride (5 M)
610.0 μl of water (100 v/v)
C5
  • Magnesium chloride 0.2 M -
  • Pentaerythritol ethoxylate (3/4 EO/OH) 35 v/v -
NA
100.0 μl of Magnesium chloride (2 M)
350.0 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
550.0 μl of water (100 v/v)
C6
  • Ethanol 15 v/v -
  • Pentaerythritol Propoxylate (5/4 PO/OH) 40 v/v -
NA
150.0 μl of Ethanol (100 v/v)
400.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
450.0 μl of water (100 v/v)
C7
  • tris chloride BCC 0.1 M pH 8
  • Pentaerythritol Propoxylate (5/4 PO/OH) 50 v/v -
pH 8
500.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
C8
  • tris chloride BCC 0.1 M pH 8
  • Sodium chloride 0.2 M -
  • Polyethylene glycol 4,000 10 w/v -
  • Polyvinylpyrrolidone K15 12.5 w/v -
pH 8
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
250.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
410.0 μl of water (100 v/v)
C9
  • Dimethyl sulfoxide 10 v/v -
  • Sodium chloride 0.1 M -
  • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
NA
333.3 μl of Dimethyl sulfoxide (30 v/v)
500.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
20.0 μl of Sodium chloride (5 M)
146.7 μl of water (100 v/v)
C10
  • Poly(acrylic acid sodium salt) 2,100 35 w/v -
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
142.9 μl of water (100 v/v)
C11
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
  • Magnesium formate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Magnesium formate (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
C12
  • Poly(acrylic acid-co-maleic acid) 24 v/v -
  • Potassium acetate 0.2 M -
NA
480.0 μl of Poly(acrylic acid-co-maleic acid) (50 v/v)
40.0 μl of Potassium acetate (5 M)
480.0 μl of water (100 v/v)
D1
  • Polypropylene glycol 400 60 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
600.0 μl of Polypropylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D2
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
  • Polyvinylpyrrolidone K15 6 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
120.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
180.0 μl of water (100 v/v)
D3
  • Polypropylene glycol 400 45 v/v -
  • Ethanol 10 v/v -
NA
100.0 μl of Ethanol (100 v/v)
450.0 μl of Polypropylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
D4
  • Pentaerythritol ethoxylate (3/4 EO/OH) 10 v/v -
  • 1-Butanol 10 v/v -
NA
100.0 μl of 1-Butanol (100 v/v)
100.0 μl of Pentaerythritol ethoxylate (3/4 EO/OH) (100 v/v)
800.0 μl of water (100 v/v)
D5
  • PPGBA 2000 6 v/v -
  • Poly(acrylic acid sodium salt) 2,100 12.5 w/v -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
120.0 μl of PPGBA 2000 (50 v/v)
250.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
530.0 μl of water (100 v/v)
D6
  • HEPES 0.1 M pH 6.5
  • Polyvinylpyrrolidone K15 6 w/v -
pH 6.5
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
120.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
780.0 μl of water (100 v/v)
D7
  • HEPES 0.1 M pH 6.5
  • Jeffamine® ED-2003 20 v/v -
pH 6.5
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
400.0 μl of Jeffamine® ED-2003 pH 7 (50 v/v)
500.0 μl of water (100 v/v)
D8
  • Glycerol Ethoxylate 20 v/v -
  • Tetrahydrofuran 10 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of Glycerol Ethoxylate (50 v/v)
200.0 μl of Tetrahydrofuran (50 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
D9
  • PPGBA 2000 25 v/v -
  • Imidazole 0.2 M pH 7
pH 7
105.9 μl of Imidazole pH 6.2 (1 M)
94.1 μl of Imidazole pH 8 (1 M)
500.0 μl of PPGBA 2000 (50 v/v)
300.0 μl of water (100 v/v)
D10
  • PPGBA 230 30 v/v -
  • Potassium chloride 0.2 M -
  • HEPES 0.1 M pH 6.5
pH 6.5
63.0 μl of HEPES pH 5 (1 M)
37.0 μl of HEPES pH 7 (1 M)
600.0 μl of PPGBA 230 (50 v/v)
50.0 μl of Potassium chloride (4 M)
250.0 μl of water (100 v/v)
D11
  • Polypropylene glycol 400 30 v/v -
  • Sodium chloride 0.1 M -
NA
300.0 μl of Polypropylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
D12
  • PPGBA 400 20 v/v -
  • 1-Propanol 15 v/v -
NA
375.0 μl of 1-Propanol (40 v/v)
400.0 μl of PPGBA 400 (50 v/v)
225.0 μl of water (100 v/v)
E1
  • PPGBA 400 15 v/v -
  • tri-Lithium citrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of PPGBA 400 (50 v/v)
66.7 μl of tri-Lithium citrate (1.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
533.3 μl of water (100 v/v)
E2
  • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
  • Potassium acetate 0.2 M -
NA
350.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
40.0 μl of Potassium acetate (5 M)
610.0 μl of water (100 v/v)
E3
  • Glycine 0.1 M pH 9.5
  • Potassium chloride 0.2 M -
  • Pentaerythritol ethoxylate (15/4 EO/OH) 20 v/v -
pH 9.5
58.6 μl of Glycine pH 9 (1 M)
41.4 μl of Glycine pH 10 (1 M)
400.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
50.0 μl of Potassium chloride (4 M)
450.0 μl of water (100 v/v)
E4
  • Pentaerythritol Propoxylate (5/4 PO/OH) 40 v/v -
  • Sodium thiocyanate 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (100 v/v)
25.0 μl of Sodium thiocyanate (8 M)
475.0 μl of water (100 v/v)
E5
  • Sokalan® CP45 25 v/v -
NA
500.0 μl of Sokalan® CP45 (50 v/v)
500.0 μl of water (100 v/v)
E6
  • Pentaerythritol ethoxylate (15/4 EO/OH) 15 v/v -
  • Potassium acetate 0.2 M -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
300.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
40.0 μl of Potassium acetate (5 M)
460.0 μl of water (100 v/v)
E7
  • Poly(acrylic acid sodium salt) 2,100 30 w/v -
  • di-Sodium malonate 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
33.3 μl of di-Sodium malonate pH 7 (3 M)
266.7 μl of water (100 v/v)
E8
  • PPGBA 230 10 v/v -
  • Jeffamine® M-600 10 v/v -
  • Ethanol 10 v/v -
NA
100.0 μl of Ethanol (100 v/v)
100.0 μl of Jeffamine® M-600 (100 v/v)
200.0 μl of PPGBA 230 (50 v/v)
600.0 μl of water (100 v/v)
E9
  • Jeffamine® ED-2003 25 w/v -
  • Lithium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
500.0 μl of Jeffamine® ED-2003 pH 7 (50 w/v)
50.0 μl of Lithium sulfate (2 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
350.0 μl of water (100 v/v)
E10
  • Sokalan® PA25 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of Sokalan® PA25 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
E11
  • Poly(acrylic acid sodium salt) 2,100 25 w/v -
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 6.5
pH 6.5
82.3 μl of HEPES pH 5 (1 M)
17.7 μl of HEPES pH 7.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
500.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
350.0 μl of water (100 v/v)
E12
  • Poly(acrylic acid sodium salt) 2,100 15 w/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
50.0 μl of Magnesium chloride (4 M)
300.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
550.0 μl of water (100 v/v)
F1
  • HEPES 0.1 M pH 6.5
  • PPGBA 2000 40 v/v -
pH 6.5
82.3 μl of HEPES pH 5 (1 M)
17.7 μl of HEPES pH 7.5 (1 M)
800.0 μl of PPGBA 2000 (50 v/v)
100.0 μl of water (100 v/v)
F2
  • Poly(acrylic acid sodium salt) 2,100 10 w/v -
  • Sodium chloride 0.5 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
100.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
600.0 μl of water (100 v/v)
F3
  • PPGBA 230 10 v/v -
  • Potassium Sodium phosphate 0.1 M pH 7
  • PPGBA 400 15 v/v -
pH 7
200.0 μl of PPGBA 230 (50 v/v)
300.0 μl of PPGBA 400 (50 v/v)
3.0 μl of Potassium Sodium phosphate pH 5.5 (3 M)
30.3 μl of Potassium Sodium phosphate pH 7.5 (3 M)
466.7 μl of water (100 v/v)
F4
  • Poly(acrylic acid sodium salt) 2,100 20 w/v -
  • Sodium chloride 0.2 M -
  • BICINE 0.1 M pH 9
pH 9
9.5 μl of BICINE pH 7.4 (1 M)
90.5 μl of BICINE pH 9.3 (1 M)
400.0 μl of Poly(acrylic acid sodium salt) 2,100 (50 w/v)
40.0 μl of Sodium chloride (5 M)
460.0 μl of water (100 v/v)
F5
  • PPGBA 2000 20 v/v -
  • di-Sodium malonate 0.2 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
400.0 μl of PPGBA 2000 (50 v/v)
66.7 μl of di-Sodium malonate pH 7 (3 M)
433.3 μl of water (100 v/v)
F6
  • Sokalan® CP45 25 v/v -
  • Cesium chloride 0.1 M -
NA
100.0 μl of Cesium chloride (1 M)
500.0 μl of Sokalan® CP45 (50 v/v)
400.0 μl of water (100 v/v)
F7
  • Sokalan® PA25 25 w/v -
NA
500.0 μl of Sokalan® PA25 (50 w/v)
500.0 μl of water (100 v/v)
F8
  • PPGBA 400 30 v/v -
  • Lithium nitrate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Lithium nitrate (8 M)
600.0 μl of PPGBA 400 (50 v/v)
275.0 μl of water (100 v/v)
F9
  • Poly(acrylic acid sodium salt) 5,100 20 w/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
400.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
500.0 μl of water (100 v/v)
F10
  • Ethylene imine polymer, branched 28 v/v -
  • HEPES 0.1 M pH 7
pH 7
560.0 μl of Ethylene imine polymer, branched (50 v/v)
100.0 μl of HEPES pH 7 (1 M)
340.0 μl of water (100 v/v)
F11
  • Sokalan® CP7 20 v/v -
  • Ammonium formate 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
10.0 μl of Ammonium formate (10 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Sokalan® CP7 (50 v/v)
490.0 μl of water (100 v/v)
F12
  • Sokalan® HP56 20 w/v -
  • Sodium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of Sodium sulfate (1 M)
400.0 μl of Sokalan® HP56 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
300.0 μl of water (100 v/v)
G1
  • Sokalan® CP7 25 v/v -
  • Potassium chloride 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
25.0 μl of Potassium chloride (4 M)
500.0 μl of Sokalan® CP7 (50 v/v)
375.0 μl of water (100 v/v)
G2
  • Sokalan® CP5 20 v/v -
  • Ammonium formate 0.3 M -
  • HEPES 0.1 M pH 7
pH 7
30.0 μl of Ammonium formate (10 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Sokalan® CP5 (50 v/v)
470.0 μl of water (100 v/v)
G3
  • Glycerol Ethoxylate 40 v/v -
NA
800.0 μl of Glycerol Ethoxylate (50 v/v)
200.0 μl of water (100 v/v)
G4
  • tris chloride BCC 0.1 M pH 8.5
  • Glycerol Ethoxylate 30 v/v -
pH 8.5
600.0 μl of Glycerol Ethoxylate (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G5
  • Polypropylene glycol 400 55 v/v -
NA
550.0 μl of Polypropylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
G6
  • Glycerol Ethoxylate 35 v/v -
  • tri-Lithium citrate 0.2 M -
NA
700.0 μl of Glycerol Ethoxylate (50 v/v)
133.3 μl of tri-Lithium citrate (1.5 M)
166.7 μl of water (100 v/v)
G7
  • Glycerol Ethoxylate 30 v/v -
  • Ammonium acetate 0.2 M -
  • MES 0.1 M pH 6.5
pH 6.5
66.7 μl of Ammonium acetate (3 M)
600.0 μl of Glycerol Ethoxylate (50 v/v)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
133.3 μl of water (100 v/v)
G8
  • Sokalan® CP42 20 v/v -
  • Methanol 5 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
100.0 μl of Methanol (50 v/v)
400.0 μl of Sokalan® CP42 (50 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)
G9
  • Sokalan® CP42 25 v/v -
  • Tetrahydrofuran 10 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
500.0 μl of Sokalan® CP42 (50 v/v)
200.0 μl of Tetrahydrofuran (50 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
200.0 μl of water (100 v/v)
G10
  • Sokalan® CP42 20 v/v -
  • Lithium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 6
pH 6
63.5 μl of BIS-TRIS pH 5.5 (1 M)
36.5 μl of BIS-TRIS pH 6.5 (1 M)
20.0 μl of Lithium acetate (5 M)
400.0 μl of Sokalan® CP42 (50 v/v)
480.0 μl of water (100 v/v)
G11
  • PPGBA 400 20 v/v -
  • BIS-TRIS 0.1 M pH 5.5
  • Sodium chloride 0.1 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
400.0 μl of PPGBA 400 (50 v/v)
100.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
G12
  • Sokalan® CP5 15 v/v -
  • BIS-TRIS 0.1 M pH 6
pH 6
63.5 μl of BIS-TRIS pH 5.5 (1 M)
36.5 μl of BIS-TRIS pH 6.5 (1 M)
300.0 μl of Sokalan® CP5 (50 v/v)
600.0 μl of water (100 v/v)
H1
  • Sokalan® CP42 25 v/v -
  • BIS-TRIS 0.1 M pH 6
pH 6
63.5 μl of BIS-TRIS pH 5.5 (1 M)
36.5 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Sokalan® CP42 (50 v/v)
400.0 μl of water (100 v/v)
H2
  • PPGBA 400 25 v/v -
  • Ammonium formate 0.2 M -
NA
80.0 μl of Ammonium formate (2.5 M)
500.0 μl of PPGBA 400 (50 v/v)
420.0 μl of water (100 v/v)
H3
  • Glycerol Ethoxylate 20 v/v -
  • Ethylene imine polymer 3 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
60.0 μl of Ethylene imine polymer (50 v/v)
400.0 μl of Glycerol Ethoxylate (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
440.0 μl of water (100 v/v)
H4
  • Glycerol Ethoxylate 25 v/v -
  • Ammonium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
40.0 μl of Ammonium chloride (5 M)
500.0 μl of Glycerol Ethoxylate (50 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of water (100 v/v)
H5
  • Sokalan® CP42 10 w/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Sokalan® CP42 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
700.0 μl of water (100 v/v)
H6
  • Poly(acrylic acid sodium salt) 5,100 30 w/v -
  • Ethanol 10 v/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of Ethanol (100 v/v)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
600.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
100.0 μl of water (100 v/v)
H7
  • Sokalan® CP42 15 v/v -
  • tri-Potassium citrate 0.2 M -
NA
300.0 μl of Sokalan® CP42 (50 v/v)
80.0 μl of tri-Potassium citrate (2.5 M)
620.0 μl of water (100 v/v)
H8
  • Sokalan® CP42 30 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Sokalan® CP42 (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H9
  • Sokalan® HP56 25 w/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
66.7 μl of Ammonium acetate (3 M)
100.0 μl of HEPES pH 7 (1 M)
500.0 μl of Sokalan® HP56 (50 w/v)
333.3 μl of water (100 v/v)
H10
  • Sokalan® CP5 25 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
500.0 μl of Sokalan® CP5 (50 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
H11
  • Polyvinylpyrrolidone K15 10 w/v -
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium formate 0.2 M -
NA
20.0 μl of Ammonium formate (10 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
380.0 μl of water (100 v/v)
H12
  • Polyvinylpyrrolidone K15 15 w/v -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 5,000 25 w/v -
pH 8
500.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
300.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
100.0 μl of water (100 v/v)