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Supplemental Information

Screen Description

MemChannel

Created 2018-03-08.

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MemChannel

    Screen Structure

    MemChannel

    Well Count 96
    Number of distinct ingredients 65
    Internal diversity (score mean dist) 0.935

    Screen Composition

    2025-06-14T13:52:14.634089 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemChannel
    Buffer 116
    Organic (non-volatile) 43
    Polymer 102
    Salt 127

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemChannel Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium Phosphate 0.05 M -
    • Sodium acetate 0.025 M pH 5
    • Polyethylene glycol 300 23 v/v -
    pH 5
    50 μl of Lithium Phosphate (1 M)
    17 μl of Sodium acetate pH 5.5 (1 M)
    230 μl of Polyethylene glycol 300 (100 v/v)
    7 μl of Sodium acetate pH 4 (1 M)
    695 μl of water (100 v/v)
    A2
    • Potassium thiocyanate 0.3 M -
    • CHES 0.1 M pH 9
    • Polyethylene glycol 3,350 15 w/v -
    pH 9
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    37 μl of Potassium thiocyanate (8 M)
    100 μl of CHES pH 9 (1 M)
    562 μl of water (100 v/v)
    A3
    • Sodium chloride 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 25 v/v -
    pH 7.5
    25 μl of HEPES pH 7 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of HEPES pH 8 (1 M)
    20 μl of Sodium chloride (5 M)
    680 μl of water (100 v/v)
    A4
    • Sodium chloride 1 M -
    • tri-Sodium citrate 0.05 M pH 6
    • Polyethylene glycol 400 32 v/v -
    pH 6
    320 μl of Polyethylene glycol 400 (100 v/v)
    1 μl of tri-Sodium citrate pH 5.6 (1 M)
    48 μl of tri-Sodium citrate pH 7 (1 M)
    200 μl of Sodium chloride (5 M)
    430 μl of water (100 v/v)
    A5
    • Potassium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Potassium chloride (1 M)
    500 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 4,000 6 w/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    120 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of MES pH 6.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    730 μl of water (100 v/v)
    A7
    • Potassium nitrate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 3,350 17 w/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    340 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    200 μl of Potassium nitrate (1 M)
    360 μl of water (100 v/v)
    A8
    • SPG buffer 0.1 M pH 8.1
    • Polyethylene glycol 1,500 20 w/v -
    pH 8.1
    99 μl of SPG buffer pH 9 (1 M)
    0 μl of SPG buffer pH 8 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    500 μl of water (100 v/v)
    A9
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 20 v/v -
    pH 7.5
    100 μl of Sodium chloride (1 M)
    400 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    50 μl of HEPES pH 7 (1 M)
    100 μl of Magnesium chloride (1 M)
    50 μl of HEPES pH 8 (1 M)
    300 μl of water (100 v/v)
    A10
    • Zinc sulfate 0.2 M -
    • Sodium acetate 0.05 M pH 4.4
    • Polyethylene glycol 4,000 15 w/v -
    pH 4.4
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    10 μl of Sodium acetate pH 5.5 (1 M)
    200 μl of Zinc sulfate (1 M)
    39 μl of Sodium acetate pH 4 (1 M)
    450 μl of water (100 v/v)
    A11
    • Sodium thiocyanate 0.4 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 4,000 8 w/v -
    pH 4
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Sodium thiocyanate (2 M)
    100 μl of Sodium acetate pH 4 (1 M)
    540 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.05 M pH 5.6
    • Polyethylene glycol 2,000 10 w/v -
    pH 5.6
    2 μl of tri-Sodium citrate pH 3.1 (1 M)
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    47 μl of tri-Sodium citrate pH 6.5 (1 M)
    750 μl of water (100 v/v)
    B1
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 22 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of HEPES pH 7 (1 M)
    50 μl of HEPES pH 8 (1 M)
    100 μl of Ammonium sulfate (1 M)
    360 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    600 μl of water (100 v/v)
    B3
    • Ammonium di-hydrogen phosphate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.7
    • Polyethylene glycol 1,500 20 w/v -
    pH 6.7
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    71 μl of BIS-TRIS pH 7 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    28 μl of BIS-TRIS pH 6 (1 M)
    300 μl of water (100 v/v)
    B4
    • Ammonium chloride 0.2 M -
    • MOPS 0.05 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 7.5
    200 μl of Ammonium chloride pH 6.3 (1 M)
    50 μl of MOPS pH 7.5 (1 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    0 μl of MOPS pH 6.5 (1 M)
    410 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.01 M -
    • Magnesium acetate 0.045 M -
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 4,000 2.5 w/v -
    • Polyethylene glycol 8,000 2.5 w/v -
    pH 7.5
    50 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of HEPES pH 7 (1 M)
    45 μl of Magnesium acetate (1 M)
    10 μl of Magnesium chloride (1 M)
    40 μl of HEPES pH 8 (1 M)
    50 μl of Polyethylene glycol 8,000 (50 w/v)
    365 μl of water (100 v/v)
    B6
    • di-Sodium malonate 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    200 μl of di-Sodium malonate pH 7 (1 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    0 μl of HEPES pH 8 (1 M)
    300 μl of water (100 v/v)
    B7
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.05 M pH 6.7
    • Polyethylene glycol 400 25 v/v -
    pH 6.7
    500 μl of Polyethylene glycol 400 (50 v/v)
    35 μl of BIS-TRIS pH 7 (1 M)
    14 μl of BIS-TRIS pH 6 (1 M)
    200 μl of Ammonium sulfate (1 M)
    250 μl of water (100 v/v)
    B8
    • Nickel sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.8
    • Polyethylene glycol 400 30 v/v -
    pH 8.8
    46 μl of tris chloride BCC pH 9 (1 M)
    600 μl of Polyethylene glycol 400 (50 v/v)
    3 μl of tris chloride BCC pH 7 (1 M)
    12 μl of Nickel sulfate (4 M)
    337 μl of water (100 v/v)
    B9
    • Ammonium sulfate 0.2 M -
    • Sodium chloride 0.02 M -
    • tri-Sodium citrate 0.1 M pH 3.7
    • Polyethylene glycol 400 19 v/v -
    pH 3.7
    80 μl of tri-Sodium citrate pH 6.5 (1 M)
    380 μl of Polyethylene glycol 400 (50 v/v)
    20 μl of Sodium chloride (1 M)
    19 μl of tri-Sodium citrate pH 3.1 (1 M)
    200 μl of Ammonium sulfate (1 M)
    300 μl of water (100 v/v)
    B10
    • Magnesium acetate 0.075 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,500 22 w/v -
    • 2-methyl-2,4-pentanediol BCC 2 v/v -
    pH 8.5
    78 μl of tris chloride BCC pH 9 (1 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    75 μl of Magnesium acetate (1 M)
    133 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    251 μl of water (100 v/v)
    B11
    • Sodium chloride 0.1 M -
    • CHES 0.1 M pH 9
    • Polyethylene glycol 600 16 v/v -
    pH 9
    100 μl of Sodium chloride (1 M)
    100 μl of CHES pH 9 (1 M)
    160 μl of Polyethylene glycol 600 (100 v/v)
    640 μl of water (100 v/v)
    B12
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 4,000 11 w/v -
    pH 5
    90 μl of tri-Sodium citrate pH 6.5 (1 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    9 μl of tri-Sodium citrate pH 3.1 (1 M)
    100 μl of Magnesium chloride (1 M)
    580 μl of water (100 v/v)
    C1
    • Zinc chloride 0.12 M -
    • tri-Potassium citrate 0.1 M pH 4
    • Polyethylene glycol 300 42 v/v -
    pH 4
    120 μl of Zinc chloride (1 M)
    420 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tri-Potassium citrate pH 4 (1 M)
    360 μl of water (100 v/v)
    C2
    • Calcium chloride 0.1 M -
    • Sodium cacodylate 0.05 M pH 5.5
    • Polyethylene glycol 400 29 v/v -
    pH 5.5
    290 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium cacodylate pH 5.5 (1 M)
    100 μl of Calcium chloride (1 M)
    560 μl of water (100 v/v)
    C3
    • Magnesium nitrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    54 μl of tris chloride BCC pH 7 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Magnesium nitrate (1 M)
    500 μl of water (100 v/v)
    C4
    • Potassium thiocyanate 0.2 M -
    • Calcium chloride 0.01 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 14 w/v -
    pH 7.5
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    10 μl of Calcium chloride (1 M)
    25 μl of Potassium thiocyanate (8 M)
    50 μl of HEPES pH 8 (1 M)
    585 μl of water (100 v/v)
    C5
    • ADA 0.05 M pH 7
    • Polyethylene glycol 1,500 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7
    50 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    550 μl of water (100 v/v)
    C6
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    C7
    • Lithium nitrate 0.125 M -
    • Glycine 0.1 M pH 9.8
    • Polyethylene glycol 400 45 v/v -
    pH 9.8
    23 μl of Glycine pH 9 (1 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    76 μl of Glycine pH 10 (1 M)
    15 μl of Lithium nitrate (8 M)
    434 μl of water (100 v/v)
    C8
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 6
    • Polyethylene glycol 400 10 v/v -
    • Polyethylene glycol 2,000 18 w/v -
    pH 6
    97 μl of tri-Sodium citrate pH 6.5 (1 M)
    100 μl of Sodium chloride (1 M)
    100 μl of Polyethylene glycol 400 (100 v/v)
    2 μl of tri-Sodium citrate pH 3.1 (1 M)
    360 μl of Polyethylene glycol 2,000 (50 w/v)
    200 μl of Ammonium sulfate (1 M)
    140 μl of water (100 v/v)
    C9
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 20 v/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    200 μl of Lithium sulfate (1 M)
    100 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    C10
    • Ammonium sulfate 0.27 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 3,350 15 w/v -
    pH 9
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    270 μl of Ammonium sulfate (1 M)
    330 μl of water (100 v/v)
    C11
    • Sodium chloride 0.125 M -
    • Lithium sulfate 0.16 M -
    • HEPES 0.075 M pH 8
    • Polyethylene glycol 300 14 v/v -
    pH 8
    125 μl of Sodium chloride (1 M)
    160 μl of Lithium sulfate (1 M)
    75 μl of HEPES pH 8 (1 M)
    140 μl of Polyethylene glycol 300 (100 v/v)
    500 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 0.05 M -
    • tri-Potassium citrate 0.05 M -
    • ADA 0.1 M pH 7
    • Polyethylene glycol 400 12 v/v -
    pH 7
    100 μl of ADA pH 7 (1 M)
    240 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of tri-Sodium citrate (1 M)
    33 μl of tri-Potassium citrate (1.5 M)
    576 μl of water (100 v/v)
    D1
    • Sodium chloride 0.5 M -
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 38 v/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    380 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Calcium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    100 μl of Sodium chloride (5 M)
    370 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.05 M -
    • Potassium di-hydrogen phosphate 0.2 M -
    • BIS-TRIS 0.08 M pH 6
    • Polyethylene glycol 4,000 10 w/v -
    pH 6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Potassium di-hydrogen phosphate (1 M)
    0 μl of BIS-TRIS pH 7 (1 M)
    50 μl of tri-Sodium citrate (1 M)
    80 μl of BIS-TRIS pH 6 (1 M)
    470 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.05 M -
    • Sodium di-hydrogen phosphate 0.05 M -
    • tri-Sodium citrate 0.08 M pH 4.5
    • Polyethylene glycol 1,000 15 w/v -
    pH 4.5
    50 μl of Lithium sulfate (1 M)
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    80 μl of tri-Sodium citrate pH 4.5 (1 M)
    16 μl of Sodium di-hydrogen phosphate (3 M)
    553 μl of water (100 v/v)
    D4
    • Potassium di-hydrogen phosphate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 35 v/v -
    pH 6.5
    100 μl of ADA pH 6.5 (1 M)
    350 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
    450 μl of water (100 v/v)
    D5
    • di-Sodium succinate 0.1 M pH 7
    • Polyethylene glycol 3,350 15 w/v -
    pH 7
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Sodium succinate pH 7 (1 M)
    600 μl of water (100 v/v)
    D6
    • Sodium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 10,000 23 w/v -
    pH 8.5
    78 μl of tris chloride BCC pH 9 (1 M)
    460 μl of Polyethylene glycol 10,000 (50 w/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Sodium sulfate (1 M)
    340 μl of water (100 v/v)
    D7
    • Lithium sulfate 0.1 M -
    • MOPS 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • Polyethylene glycol 20,000 10 w/v -
    pH 7.5
    100 μl of MOPS pH 7.5 (1 M)
    100 μl of Lithium sulfate (1 M)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    266 μl of water (100 v/v)
    D8
    • Magnesium chloride 0.3 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 2,000 24 w/v -
    pH 9
    300 μl of Magnesium chloride pH 7 (1 M)
    480 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of BICINE pH 9 (1 M)
    120 μl of water (100 v/v)
    D9
    • Sodium chloride 0.1 M -
    • Ammonium formate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 350 22 v/v -
    pH 8.5
    78 μl of tris chloride BCC pH 9 (1 M)
    100 μl of Sodium chloride (1 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    220 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    20 μl of Ammonium formate (2.5 M)
    560 μl of water (100 v/v)
    D10
    • Calcium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 5 w/v -
    pH 7.5
    50 μl of HEPES pH 7 (1 M)
    100 μl of Calcium chloride (1 M)
    50 μl of HEPES pH 8 (1 M)
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    700 μl of water (100 v/v)
    D11
    • Nickel (II) chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    pH 4
    0 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Nickel (II) chloride (1 M)
    100 μl of Sodium acetate pH 4 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    610 μl of water (100 v/v)
    D12
    • Lithium nitrate 0.05 M -
    • ADA 0.1 M pH 6.2
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 6.2
    57 μl of ADA pH 6.5 (1 M)
    42 μl of ADA pH 5.5 (1 M)
    6 μl of Lithium nitrate (8 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    633 μl of water (100 v/v)
    E1
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.7
    • Polyethylene glycol monomethyl ether 550 36 v/v -
    pH 8.7
    89 μl of tris chloride BCC pH 9 (1 M)
    10 μl of tris chloride BCC pH 7 (1 M)
    360 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Magnesium chloride (1 M)
    440 μl of water (100 v/v)
    E2
    • Potassium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 36 v/v -
    pH 6.5
    0 μl of MES pH 5.5 (1 M)
    100 μl of Potassium chloride (1 M)
    360 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    440 μl of water (100 v/v)
    E3
    • Sodium chloride 0.15 M -
    • tri-Sodium citrate 0.05 M pH 3.7
    • Polyethylene glycol monomethyl ether 2,000 19 w/v -
    • 2-methyl-2,4-pentanediol BCC 6 v/v -
    pH 3.7
    40 μl of tri-Sodium citrate pH 6.5 (1 M)
    120 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    150 μl of Sodium chloride (1 M)
    9 μl of tri-Sodium citrate pH 3.1 (1 M)
    380 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of water (100 v/v)
    E4
    • Ammonium sulfate 0.125 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    125 μl of Ammonium sulfate (1 M)
    525 μl of water (100 v/v)
    E5
    • Magnesium nitrate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 1,500 16 w/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    320 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Magnesium nitrate (1 M)
    480 μl of water (100 v/v)
    E6
    • Cadmium chloride 0.002 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 28 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Cadmium chloride (0.1 M)
    600 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.34 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 10 w/v -
    pH 5.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    340 μl of Ammonium sulfate (1 M)
    360 μl of water (100 v/v)
    E8
    • Calcium acetate 0.1 M -
    • Sodium chloride 0.075 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 24 v/v -
    pH 7
    75 μl of Sodium chloride (1 M)
    44 μl of MOPS pH 7.5 (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium acetate (1 M)
    55 μl of MOPS pH 6.5 (1 M)
    485 μl of water (100 v/v)
    E9
    • Magnesium chloride 0.25 M -
    • MOPS 0.05 M pH 6.5
    • Polyethylene glycol 2,000 23 w/v -
    pH 6.5
    0 μl of MOPS pH 7.5 (1 M)
    460 μl of Polyethylene glycol 2,000 (50 w/v)
    50 μl of MOPS pH 6.5 (1 M)
    250 μl of Magnesium chloride (1 M)
    240 μl of water (100 v/v)
    E10
    • Sodium chloride 0.04 M -
    • Calcium chloride 0.01 M -
    • Sodium acetate 0.035 M pH 5.3
    • Polyethylene glycol 400 20 v/v -
    pH 5.3
    400 μl of Polyethylene glycol 400 (50 v/v)
    23 μl of Sodium acetate pH 5.5 (1 M)
    40 μl of Sodium chloride (1 M)
    10 μl of Calcium chloride (1 M)
    11 μl of Sodium acetate pH 5 (1 M)
    515 μl of water (100 v/v)
    E11
    • HEPES 0.075 M pH 7
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 4,000 5 w/v -
    • Polyethylene glycol 8,000 2.5 w/v -
    pH 7
    50 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    75 μl of HEPES pH 7 (1 M)
    375 μl of water (100 v/v)
    E12
    • Magnesium nitrate 0.05 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    50 μl of Magnesium nitrate (1 M)
    450 μl of water (100 v/v)
    F1
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,350 31 w/v -
    pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Lithium sulfate (1 M)
    620 μl of Polyethylene glycol 3,350 (50 w/v)
    180 μl of water (100 v/v)
    F2
    • Magnesium acetate 0.4 M -
    • tris chloride BCC 0.08 M pH 8.8
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.8
    74 μl of tris chloride BCC pH 9 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    5 μl of tris chloride BCC pH 7 (1 M)
    400 μl of Magnesium acetate (1 M)
    280 μl of water (100 v/v)
    F3
    • Lithium nitrate 0.05 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 36 v/v -
    pH 6
    53 μl of MES pH 5.5 (1 M)
    47 μl of MES pH 6.5 (1 M)
    6 μl of Lithium nitrate (8 M)
    720 μl of Polyethylene glycol 600 (50 v/v)
    173 μl of water (100 v/v)
    F4
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 26 v/v -
    pH 5
    520 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Lithium sulfate (1 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    280 μl of water (100 v/v)
    F5
    • Calcium chloride 0.125 M -
    • tris chloride BCC 0.02 M pH 7.4
    • Polyethylene glycol 3,000 16 w/v -
    pH 7.4
    2 μl of tris chloride BCC pH 9 (1 M)
    17 μl of tris chloride BCC pH 7 (1 M)
    125 μl of Calcium chloride (1 M)
    320 μl of Polyethylene glycol 3,000 (50 w/v)
    535 μl of water (100 v/v)
    F6
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 40 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Calcium chloride (1 M)
    300 μl of water (100 v/v)
    F7
    • Sodium chloride 0.04 M -
    • Calcium chloride 0.125 M -
    • MES 0.035 M pH 5.5
    • Polyethylene glycol 400 20 v/v -
    pH 5.5
    35 μl of MES pH 5.5 (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of Sodium chloride (1 M)
    125 μl of Calcium chloride (1 M)
    400 μl of water (100 v/v)
    F8
    • Magnesium chloride 0.02 M -
    • ADA 0.06 M pH 7
    • Polyethylene glycol 1,500 12 w/v -
    pH 7
    60 μl of ADA pH 7 (1 M)
    20 μl of Magnesium chloride (1 M)
    240 μl of Polyethylene glycol 1,500 (50 w/v)
    680 μl of water (100 v/v)
    F9
    • Nickel (II) chloride 0.01 M -
    • Sodium cacodylate 0.05 M pH 5.5
    • Polyethylene glycol 400 42 v/v -
    pH 5.5
    420 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium cacodylate pH 5.5 (1 M)
    10 μl of Nickel (II) chloride (1 M)
    520 μl of water (100 v/v)
    F10
    • Potassium chloride 0.055 M -
    • MOPS 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 11 w/v -
    pH 7
    50 μl of MOPS pH 7 (1 M)
    55 μl of Potassium chloride (1 M)
    220 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    675 μl of water (100 v/v)
    F11
    • Magnesium chloride 0.075 M -
    • HEPES 0.1 M pH 8
    • Polyethylene glycol 1,500 22 w/v -
    pH 8
    75 μl of Magnesium chloride (1 M)
    100 μl of HEPES pH 8 (1 M)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    385 μl of water (100 v/v)
    F12
    • Lithium chloride 0.1 M -
    • Glycine 0.05 M pH 9.5
    • Polyethylene glycol 400 40 v/v -
    pH 9.5
    50 μl of Glycine pH 9.5 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium chloride (1 M)
    450 μl of water (100 v/v)
    G1
    • Sodium acetate 0.325 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 20 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    162 μl of Sodium acetate (2 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    537 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.25 M -
    • MES 0.1 M pH 5.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 v/v -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    350 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (100 v/v)
    250 μl of Ammonium sulfate (1 M)
    300 μl of water (100 v/v)
    G3
    • tri-Potassium citrate 0.1 M pH 4
    • Polyethylene glycol 400 17 v/v -
    pH 4
    340 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of tri-Potassium citrate pH 4 (1 M)
    560 μl of water (100 v/v)
    G4
    • Sodium chloride 0.07 M -
    • tri-Sodium citrate 0.075 M pH 3.7
    • Polyethylene glycol 400 23 v/v -
    pH 3.7
    70 μl of Sodium chloride (1 M)
    60 μl of tri-Sodium citrate pH 4 (1 M)
    14 μl of tri-Sodium citrate pH 3.5 (1 M)
    460 μl of Polyethylene glycol 400 (50 v/v)
    395 μl of water (100 v/v)
    G5
    • Barium chloride 0.15 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 26 v/v -
    pH 7
    150 μl of Barium chloride (1 M)
    520 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    230 μl of water (100 v/v)
    G6
    • Ammonium formate 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 25 v/v -
    pH 6
    53 μl of MES pH 5.5 (1 M)
    200 μl of Ammonium formate (5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    47 μl of MES pH 6.5 (1 M)
    450 μl of water (100 v/v)
    G7
    • Calcium chloride 0.06 M -
    • Magnesium chloride 0.06 M -
    • CHES 0.075 M pH 9
    • Polyethylene glycol 4,000 22 w/v -
    pH 9
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    60 μl of Calcium chloride (1 M)
    60 μl of Magnesium chloride (1 M)
    75 μl of CHES pH 9 (1 M)
    365 μl of water (100 v/v)
    G8
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 28 v/v -
    pH 6
    53 μl of MES pH 5.5 (1 M)
    47 μl of MES pH 6.5 (1 M)
    100 μl of Magnesium chloride (1 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    520 μl of water (100 v/v)
    G9
    • Ammonium thiocyanate 0.4 M -
    • tri-Sodium citrate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 4.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of Ammonium thiocyanate (1 M)
    100 μl of tri-Sodium citrate pH 4.5 (1 M)
    300 μl of water (100 v/v)
    G10
    • Sodium acetate 0.18 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 27 v/v -
    • Pentaerythritol ethoxylate (15/4 EO/OH) 12 v/v -
    pH 6
    53 μl of MES pH 5.5 (1 M)
    240 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    90 μl of Sodium acetate (2 M)
    270 μl of Polyethylene glycol 400 (100 v/v)
    47 μl of MES pH 6.5 (1 M)
    300 μl of water (100 v/v)
    G11
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium chloride (2 M)
    600 μl of water (100 v/v)
    G12
    • Magnesium acetate 0.01 M -
    • Sodium acetate 0.05 M pH 5
    • Polyethylene glycol 400 23 v/v -
    pH 5
    460 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of Sodium acetate pH 5 (1 M)
    10 μl of Magnesium acetate (1 M)
    480 μl of water (100 v/v)
    H1
    • Barium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 300 28 v/v -
    pH 8.5
    100 μl of Barium chloride (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    520 μl of water (100 v/v)
    H2
    • tri-Sodium citrate 0.05 M pH 5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5
    50 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    450 μl of water (100 v/v)
    H3
    • Sodium chloride 0.275 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 25 v/v -
    pH 7
    275 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    375 μl of water (100 v/v)
    H4
    • Sodium sulfate 0.15 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 400 27 v/v -
    pH 7
    270 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    150 μl of Sodium sulfate (1 M)
    480 μl of water (100 v/v)
    H5
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 1,500 22 w/v -
    pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    460 μl of water (100 v/v)
    H6
    • Cadmium chloride 0.001 M -
    • Glycine 0.1 M pH 9.5
    • 2-methyl-2,4-pentanediol BCC 60 v/v -
    pH 9.5
    100 μl of Glycine pH 9.5 (1 M)
    600 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Cadmium chloride (0.1 M)
    290 μl of water (100 v/v)
    H7
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 27 v/v -
    pH 8.5
    540 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    410 μl of water (100 v/v)
    H8
    • Potassium acetate 0.25 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 22 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    54 μl of tris chloride BCC pH 7 (1 M)
    50 μl of Potassium acetate (5 M)
    220 μl of Polyethylene glycol 300 (100 v/v)
    630 μl of water (100 v/v)
    H9
    • Magnesium chloride 0.125 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 14 w/v -
    pH 6
    53 μl of MES pH 5.5 (1 M)
    47 μl of MES pH 6.5 (1 M)
    280 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    125 μl of Magnesium chloride (1 M)
    495 μl of water (100 v/v)
    H10
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 600 26 v/v -
    pH 8
    22 μl of tris chloride BCC pH 9 (1 M)
    27 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Sodium chloride (2 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    490 μl of water (100 v/v)
    H11
    • Ammonium acetate 0.2 M -
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6
    • Polyethylene glycol 4,000 12 w/v -
    pH 6
    200 μl of Ammonium acetate (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of BIS-TRIS pH 6 (1 M)
    50 μl of Sodium chloride (2 M)
    410 μl of water (100 v/v)
    H12
    • tri-Sodium citrate 0.09 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    pH 5.6
    600 μl of Polyethylene glycol 400 (50 v/v)
    90 μl of tri-Sodium citrate pH 5.6 (1 M)
    310 μl of water (100 v/v)