Jump to page contents
Welcome to the new C6 website. This website aims preserve as many features as possible of the original C6 website.
This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.

Supplemental Information

Screen Description

MemChannel

Created 2018-03-08.

Export

Barcodes

MemChannel

No barcodes found.

Screen Structure

MemChannel

Well Count 96
Number of distinct ingredients 65
Internal diversity (score mean dist) 0.935

Screen Composition

2025-08-16T09:05:37.882752 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemChannel
Buffer 116
Organic (non-volatile) 43
Polymer 102
Salt 127

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemChannel Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Lithium Phosphate 0.05 M -
  • Sodium acetate 0.025 M pH 5
  • Polyethylene glycol 300 23 v/v -
pH 5
50.0 μl of Lithium Phosphate (1 M)
230.0 μl of Polyethylene glycol 300 (100 v/v)
7.9 μl of Sodium acetate pH 4 (1 M)
17.1 μl of Sodium acetate pH 5.5 (1 M)
695.0 μl of water (100 v/v)
A2
  • Potassium thiocyanate 0.3 M -
  • CHES 0.1 M pH 9
  • Polyethylene glycol 3,350 15 w/v -
pH 9
100.0 μl of CHES pH 9 (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
37.5 μl of Potassium thiocyanate (8 M)
562.5 μl of water (100 v/v)
A3
  • Sodium chloride 0.1 M -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 400 25 v/v -
pH 7.5
25.0 μl of HEPES pH 7 (1 M)
25.0 μl of HEPES pH 8 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
680.0 μl of water (100 v/v)
A4
  • Sodium chloride 1 M -
  • tri-Sodium citrate 0.05 M pH 6
  • Polyethylene glycol 400 32 v/v -
pH 6
320.0 μl of Polyethylene glycol 400 (100 v/v)
200.0 μl of Sodium chloride (5 M)
11.2 μl of tri-Sodium citrate pH 5.6 (1 M)
38.8 μl of tri-Sodium citrate pH 7 (1 M)
430.0 μl of water (100 v/v)
A5
  • Potassium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
pH 8
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium chloride (1 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
A6
  • Magnesium chloride 0.2 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 4,000 6 w/v -
pH 6.5
0.0 μl of MES pH 5.5 (1 M)
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
120.0 μl of Polyethylene glycol 4,000 (50 w/v)
730.0 μl of water (100 v/v)
A7
  • Potassium nitrate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 3,350 17 w/v -
pH 6.5
0.0 μl of MES pH 5.5 (1 M)
100.0 μl of MES pH 6.5 (1 M)
340.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of Potassium nitrate (1 M)
360.0 μl of water (100 v/v)
A8
  • SPG buffer 0.1 M pH 8.1
  • Polyethylene glycol 1,500 20 w/v -
pH 8.1
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
2.0 μl of SPG buffer pH 8 (1 M)
98.0 μl of SPG buffer pH 9 (1 M)
500.0 μl of water (100 v/v)
A9
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Pentaerythritol ethoxylate (15/4 EO/OH) 20 v/v -
pH 7.5
50.0 μl of HEPES pH 7 (1 M)
50.0 μl of HEPES pH 8 (1 M)
100.0 μl of Magnesium chloride (1 M)
400.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
100.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
A10
  • Zinc sulfate 0.2 M -
  • Sodium acetate 0.05 M pH 4.4
  • Polyethylene glycol 4,000 15 w/v -
pH 4.4
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
39.4 μl of Sodium acetate pH 4 (1 M)
10.6 μl of Sodium acetate pH 5.5 (1 M)
200.0 μl of Zinc sulfate (1 M)
450.0 μl of water (100 v/v)
A11
  • Sodium thiocyanate 0.4 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 4,000 8 w/v -
pH 4
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
200.0 μl of Sodium thiocyanate (2 M)
540.0 μl of water (100 v/v)
A12
  • tri-Sodium citrate 0.05 M pH 5.6
  • Polyethylene glycol 2,000 10 w/v -
pH 5.6
200.0 μl of Polyethylene glycol 2,000 (50 w/v)
16.9 μl of tri-Sodium citrate pH 3.1 (1 M)
33.1 μl of tri-Sodium citrate pH 6.5 (1 M)
750.0 μl of water (100 v/v)
B1
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 22 v/v -
pH 7.5
100.0 μl of Ammonium sulfate (1 M)
50.0 μl of HEPES pH 7 (1 M)
50.0 μl of HEPES pH 8 (1 M)
440.0 μl of Polyethylene glycol 400 (50 v/v)
360.0 μl of water (100 v/v)
B2
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
pH 6.5
0.0 μl of MES pH 5.5 (1 M)
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
600.0 μl of water (100 v/v)
B3
  • Ammonium di-hydrogen phosphate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.7
  • Polyethylene glycol 1,500 20 w/v -
pH 6.7
200.0 μl of Ammonium di-hydrogen phosphate (1 M)
71.8 μl of BIS-TRIS pH 7 (1 M)
28.2 μl of BIS-TRIS pH 6 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
300.0 μl of water (100 v/v)
B4
  • Ammonium chloride 0.2 M -
  • MOPS 0.05 M pH 7.5
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 7.5
200.0 μl of Ammonium chloride pH 6.3 (1 M)
-0.0 μl of MOPS pH 6.5 (1 M)
50.0 μl of MOPS pH 7.5 (1 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
410.0 μl of water (100 v/v)
B5
  • Magnesium chloride 0.01 M -
  • Magnesium acetate 0.045 M -
  • HEPES 0.08 M pH 7.5
  • Polyethylene glycol 400 20 v/v -
  • Polyethylene glycol 4,000 2.5 w/v -
  • Polyethylene glycol 8,000 2.5 w/v -
pH 7.5
40.0 μl of HEPES pH 7 (1 M)
40.0 μl of HEPES pH 8 (1 M)
45.0 μl of Magnesium acetate (1 M)
10.0 μl of Magnesium chloride (1 M)
50.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Polyethylene glycol 8,000 (50 w/v)
365.0 μl of water (100 v/v)
B6
  • di-Sodium malonate 0.2 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
-0.0 μl of HEPES pH 8 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of di-Sodium malonate pH 7 (1 M)
300.0 μl of water (100 v/v)
B7
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.05 M pH 6.7
  • Polyethylene glycol 400 25 v/v -
pH 6.7
200.0 μl of Ammonium sulfate (1 M)
35.9 μl of BIS-TRIS pH 7 (1 M)
14.1 μl of BIS-TRIS pH 6 (1 M)
500.0 μl of Polyethylene glycol 400 (50 v/v)
250.0 μl of water (100 v/v)
B8
  • Nickel sulfate 0.05 M -
  • tris chloride BCC 0.05 M pH 8.8
  • Polyethylene glycol 400 30 v/v -
pH 8.8
12.5 μl of Nickel sulfate (4 M)
600.0 μl of Polyethylene glycol 400 (50 v/v)
3.3 μl of tris chloride BCC pH 7 (1 M)
46.7 μl of tris chloride BCC pH 9 (1 M)
337.5 μl of water (100 v/v)
B9
  • Ammonium sulfate 0.2 M -
  • Sodium chloride 0.02 M -
  • tri-Sodium citrate 0.1 M pH 3.7
  • Polyethylene glycol 400 19 v/v -
pH 3.7
200.0 μl of Ammonium sulfate (1 M)
380.0 μl of Polyethylene glycol 400 (50 v/v)
20.0 μl of Sodium chloride (1 M)
65.9 μl of tri-Sodium citrate pH 3.1 (1 M)
34.1 μl of tri-Sodium citrate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
B10
  • Magnesium acetate 0.075 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,500 22 w/v -
  • 2-methyl-2,4-pentanediol BCC 2 v/v -
pH 8.5
133.3 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
75.0 μl of Magnesium acetate (1 M)
440.0 μl of Polyethylene glycol 1,500 (50 w/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
251.7 μl of water (100 v/v)
B11
  • Sodium chloride 0.1 M -
  • CHES 0.1 M pH 9
  • Polyethylene glycol 600 16 v/v -
pH 9
100.0 μl of CHES pH 9 (1 M)
160.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of Sodium chloride (1 M)
640.0 μl of water (100 v/v)
B12
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 4,000 11 w/v -
pH 5
100.0 μl of Magnesium chloride (1 M)
220.0 μl of Polyethylene glycol 4,000 (50 w/v)
47.9 μl of tri-Sodium citrate pH 3.1 (1 M)
52.1 μl of tri-Sodium citrate pH 6.5 (1 M)
580.0 μl of water (100 v/v)
C1
  • Zinc chloride 0.12 M -
  • tri-Potassium citrate 0.1 M pH 4
  • Polyethylene glycol 300 42 v/v -
pH 4
420.0 μl of Polyethylene glycol 300 (100 v/v)
120.0 μl of Zinc chloride (1 M)
100.0 μl of tri-Potassium citrate pH 4 (1 M)
360.0 μl of water (100 v/v)
C2
  • Calcium chloride 0.1 M -
  • Sodium cacodylate 0.05 M pH 5.5
  • Polyethylene glycol 400 29 v/v -
pH 5.5
100.0 μl of Calcium chloride (1 M)
290.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium cacodylate pH 5.5 (1 M)
560.0 μl of water (100 v/v)
C3
  • Magnesium nitrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
pH 8
100.0 μl of Magnesium nitrate (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
500.0 μl of water (100 v/v)
C4
  • Potassium thiocyanate 0.2 M -
  • Calcium chloride 0.01 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 14 w/v -
pH 7.5
10.0 μl of Calcium chloride (1 M)
50.0 μl of HEPES pH 7 (1 M)
50.0 μl of HEPES pH 8 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
585.0 μl of water (100 v/v)
C5
  • ADA 0.05 M pH 7
  • Polyethylene glycol 1,500 10 w/v -
  • Polyethylene glycol 1,000 10 w/v -
pH 7
50.0 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
200.0 μl of Polyethylene glycol 1,500 (50 w/v)
550.0 μl of water (100 v/v)
C6
  • HEPES 0.1 M pH 7
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 7
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7 (1 M)
250.0 μl of water (100 v/v)
C7
  • Lithium nitrate 0.125 M -
  • Glycine 0.1 M pH 9.8
  • Polyethylene glycol 400 45 v/v -
pH 9.8
23.8 μl of Glycine pH 9 (1 M)
76.2 μl of Glycine pH 10 (1 M)
15.6 μl of Lithium nitrate (8 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
434.4 μl of water (100 v/v)
C8
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol 400 10 v/v -
  • Polyethylene glycol 2,000 18 w/v -
pH 6
200.0 μl of Ammonium sulfate (1 M)
360.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
22.5 μl of tri-Sodium citrate pH 3.1 (1 M)
77.5 μl of tri-Sodium citrate pH 6.5 (1 M)
140.0 μl of water (100 v/v)
C9
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 20 v/v -
pH 6.5
200.0 μl of Lithium sulfate (1 M)
0.0 μl of MES pH 5.5 (1 M)
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 400 (50 v/v)
300.0 μl of water (100 v/v)
C10
  • Ammonium sulfate 0.27 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 3,350 15 w/v -
pH 9
270.0 μl of Ammonium sulfate (1 M)
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
330.0 μl of water (100 v/v)
C11
  • Sodium chloride 0.125 M -
  • Lithium sulfate 0.16 M -
  • HEPES 0.075 M pH 8
  • Polyethylene glycol 300 14 v/v -
pH 8
75.0 μl of HEPES pH 8 (1 M)
160.0 μl of Lithium sulfate (1 M)
140.0 μl of Polyethylene glycol 300 (100 v/v)
125.0 μl of Sodium chloride (1 M)
500.0 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.05 M -
  • tri-Potassium citrate 0.05 M -
  • ADA 0.1 M pH 7
  • Polyethylene glycol 400 12 v/v -
pH 7
100.0 μl of ADA pH 7 (1 M)
240.0 μl of Polyethylene glycol 400 (50 v/v)
33.3 μl of tri-Potassium citrate (1.5 M)
50.0 μl of tri-Sodium citrate (1 M)
576.7 μl of water (100 v/v)
D1
  • Sodium chloride 0.5 M -
  • Calcium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 38 v/v -
pH 6.5
50.0 μl of Calcium chloride (1 M)
0.0 μl of MES pH 5.5 (1 M)
100.0 μl of MES pH 6.5 (1 M)
380.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (5 M)
370.0 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.05 M -
  • Potassium di-hydrogen phosphate 0.2 M -
  • BIS-TRIS 0.08 M pH 6
  • Polyethylene glycol 4,000 10 w/v -
pH 6
-0.0 μl of BIS-TRIS pH 7 (1 M)
80.0 μl of BIS-TRIS pH 6 (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
200.0 μl of Potassium di-hydrogen phosphate (1 M)
50.0 μl of tri-Sodium citrate (1 M)
470.0 μl of water (100 v/v)
D3
  • Lithium sulfate 0.05 M -
  • Sodium di-hydrogen phosphate 0.05 M -
  • tri-Sodium citrate 0.08 M pH 4.5
  • Polyethylene glycol 1,000 15 w/v -
pH 4.5
50.0 μl of Lithium sulfate (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
16.7 μl of Sodium di-hydrogen phosphate (3 M)
80.0 μl of tri-Sodium citrate pH 4.5 (1 M)
553.3 μl of water (100 v/v)
D4
  • Potassium di-hydrogen phosphate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 35 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
450.0 μl of water (100 v/v)
D5
  • di-Sodium succinate 0.1 M pH 7
  • Polyethylene glycol 3,350 15 w/v -
pH 7
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 7 (1 M)
600.0 μl of water (100 v/v)
D6
  • Sodium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 10,000 23 w/v -
pH 8.5
460.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of Sodium sulfate (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
340.0 μl of water (100 v/v)
D7
  • Lithium sulfate 0.1 M -
  • MOPS 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 20 v/v -
  • Polyethylene glycol 20,000 10 w/v -
pH 7.5
100.0 μl of Lithium sulfate (1 M)
100.0 μl of MOPS pH 7.5 (1 M)
333.3 μl of Polyethylene glycol 20,000 (30 w/v)
200.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
266.7 μl of water (100 v/v)
D8
  • Magnesium chloride 0.3 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 2,000 24 w/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
300.0 μl of Magnesium chloride pH 7 (1 M)
480.0 μl of Polyethylene glycol 2,000 (50 w/v)
120.0 μl of water (100 v/v)
D9
  • Sodium chloride 0.1 M -
  • Ammonium formate 0.05 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 350 22 v/v -
pH 8.5
20.0 μl of Ammonium formate (2.5 M)
220.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
100.0 μl of Sodium chloride (1 M)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
560.0 μl of water (100 v/v)
D10
  • Calcium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 5 w/v -
pH 7.5
100.0 μl of Calcium chloride (1 M)
50.0 μl of HEPES pH 7 (1 M)
50.0 μl of HEPES pH 8 (1 M)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
700.0 μl of water (100 v/v)
D11
  • Nickel (II) chloride 0.05 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol monomethyl ether 2,000 12 w/v -
pH 4
50.0 μl of Nickel (II) chloride (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
0.0 μl of Sodium acetate pH 5.5 (1 M)
610.0 μl of water (100 v/v)
D12
  • Lithium nitrate 0.05 M -
  • ADA 0.1 M pH 6.2
  • Polyethylene glycol monomethyl ether 550 26 v/v -
pH 6.2
42.8 μl of ADA pH 5.5 (1 M)
57.2 μl of ADA pH 6.5 (1 M)
6.2 μl of Lithium nitrate (8 M)
260.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
633.8 μl of water (100 v/v)
E1
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.7
  • Polyethylene glycol monomethyl ether 550 36 v/v -
pH 8.7
100.0 μl of Magnesium chloride (1 M)
360.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
10.9 μl of tris chloride BCC pH 7 (1 M)
89.1 μl of tris chloride BCC pH 9 (1 M)
440.0 μl of water (100 v/v)
E2
  • Potassium chloride 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 36 v/v -
pH 6.5
0.0 μl of MES pH 5.5 (1 M)
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium chloride (1 M)
440.0 μl of water (100 v/v)
E3
  • Sodium chloride 0.15 M -
  • tri-Sodium citrate 0.05 M pH 3.7
  • Polyethylene glycol monomethyl ether 2,000 19 w/v -
  • 2-methyl-2,4-pentanediol BCC 6 v/v -
pH 3.7
120.0 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
380.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
150.0 μl of Sodium chloride (1 M)
32.9 μl of tri-Sodium citrate pH 3.1 (1 M)
17.1 μl of tri-Sodium citrate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 0.125 M -
  • ADA 0.05 M pH 6.5
  • Polyethylene glycol 4,000 15 w/v -
pH 6.5
50.0 μl of ADA pH 6.5 (1 M)
125.0 μl of Ammonium sulfate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
525.0 μl of water (100 v/v)
E5
  • Magnesium nitrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 1,500 16 w/v -
pH 5.6
100.0 μl of Magnesium nitrate (1 M)
320.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
480.0 μl of water (100 v/v)
E6
  • Cadmium chloride 0.002 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 28 v/v -
pH 7
20.0 μl of Cadmium chloride (0.1 M)
100.0 μl of HEPES pH 7 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
E7
  • Ammonium sulfate 0.34 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 10 w/v -
pH 5.6
340.0 μl of Ammonium sulfate (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
360.0 μl of water (100 v/v)
E8
  • Calcium acetate 0.1 M -
  • Sodium chloride 0.075 M -
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 400 24 v/v -
pH 7
100.0 μl of Calcium acetate (1 M)
55.9 μl of MOPS pH 6.5 (1 M)
44.1 μl of MOPS pH 7.5 (1 M)
240.0 μl of Polyethylene glycol 400 (100 v/v)
75.0 μl of Sodium chloride (1 M)
485.0 μl of water (100 v/v)
E9
  • Magnesium chloride 0.25 M -
  • MOPS 0.05 M pH 6.5
  • Polyethylene glycol 2,000 23 w/v -
pH 6.5
50.0 μl of MOPS pH 6.5 (1 M)
0.0 μl of MOPS pH 7.5 (1 M)
250.0 μl of Magnesium chloride (1 M)
460.0 μl of Polyethylene glycol 2,000 (50 w/v)
240.0 μl of water (100 v/v)
E10
  • Sodium chloride 0.04 M -
  • Calcium chloride 0.01 M -
  • Sodium acetate 0.035 M pH 5.3
  • Polyethylene glycol 400 20 v/v -
pH 5.3
10.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (50 v/v)
11.7 μl of Sodium acetate pH 5 (1 M)
23.3 μl of Sodium acetate pH 5.5 (1 M)
40.0 μl of Sodium chloride (1 M)
515.0 μl of water (100 v/v)
E11
  • HEPES 0.075 M pH 7
  • Polyethylene glycol 400 20 v/v -
  • Polyethylene glycol 4,000 5 w/v -
  • Polyethylene glycol 8,000 2.5 w/v -
pH 7
75.0 μl of HEPES pH 7 (1 M)
100.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Polyethylene glycol 8,000 (50 w/v)
375.0 μl of water (100 v/v)
E12
  • Magnesium nitrate 0.05 M -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol monomethyl ether 550 20 v/v -
pH 5.6
50.0 μl of Magnesium nitrate (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
450.0 μl of water (100 v/v)
F1
  • Lithium sulfate 0.1 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,350 31 w/v -
pH 7
100.0 μl of Lithium sulfate (1 M)
620.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
180.0 μl of water (100 v/v)
F2
  • Magnesium acetate 0.4 M -
  • tris chloride BCC 0.08 M pH 8.8
  • Polyethylene glycol 4,000 12 w/v -
pH 8.8
400.0 μl of Magnesium acetate (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
5.4 μl of tris chloride BCC pH 7 (1 M)
74.6 μl of tris chloride BCC pH 9 (1 M)
280.0 μl of water (100 v/v)
F3
  • Lithium nitrate 0.05 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 36 v/v -
pH 6
6.2 μl of Lithium nitrate (8 M)
53.0 μl of MES pH 5.5 (1 M)
47.0 μl of MES pH 6.5 (1 M)
720.0 μl of Polyethylene glycol 600 (50 v/v)
173.8 μl of water (100 v/v)
F4
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 26 v/v -
pH 5
100.0 μl of Lithium sulfate (1 M)
520.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
280.0 μl of water (100 v/v)
F5
  • Calcium chloride 0.125 M -
  • tris chloride BCC 0.02 M pH 7.4
  • Polyethylene glycol 3,000 16 w/v -
pH 7.4
125.0 μl of Calcium chloride (1 M)
320.0 μl of Polyethylene glycol 3,000 (50 w/v)
17.7 μl of tris chloride BCC pH 7 (1 M)
2.3 μl of tris chloride BCC pH 9 (1 M)
535.0 μl of water (100 v/v)
F6
  • Calcium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 40 v/v -
pH 8
200.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
300.0 μl of water (100 v/v)
F7
  • Sodium chloride 0.04 M -
  • Calcium chloride 0.125 M -
  • MES 0.035 M pH 5.5
  • Polyethylene glycol 400 20 v/v -
pH 5.5
125.0 μl of Calcium chloride (1 M)
35.0 μl of MES pH 5.5 (1 M)
400.0 μl of Polyethylene glycol 400 (50 v/v)
40.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
F8
  • Magnesium chloride 0.02 M -
  • ADA 0.06 M pH 7
  • Polyethylene glycol 1,500 12 w/v -
pH 7
60.0 μl of ADA pH 7 (1 M)
20.0 μl of Magnesium chloride (1 M)
240.0 μl of Polyethylene glycol 1,500 (50 w/v)
680.0 μl of water (100 v/v)
F9
  • Nickel (II) chloride 0.01 M -
  • Sodium cacodylate 0.05 M pH 5.5
  • Polyethylene glycol 400 42 v/v -
pH 5.5
10.0 μl of Nickel (II) chloride (1 M)
420.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium cacodylate pH 5.5 (1 M)
520.0 μl of water (100 v/v)
F10
  • Potassium chloride 0.055 M -
  • MOPS 0.05 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 11 w/v -
pH 7
50.0 μl of MOPS pH 7 (1 M)
220.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
55.0 μl of Potassium chloride (1 M)
675.0 μl of water (100 v/v)
F11
  • Magnesium chloride 0.075 M -
  • HEPES 0.1 M pH 8
  • Polyethylene glycol 1,500 22 w/v -
pH 8
100.0 μl of HEPES pH 8 (1 M)
75.0 μl of Magnesium chloride (1 M)
440.0 μl of Polyethylene glycol 1,500 (50 w/v)
385.0 μl of water (100 v/v)
F12
  • Lithium chloride 0.1 M -
  • Glycine 0.05 M pH 9.5
  • Polyethylene glycol 400 40 v/v -
pH 9.5
50.0 μl of Glycine pH 9.5 (1 M)
100.0 μl of Lithium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
G1
  • Sodium acetate 0.325 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 20 v/v -
pH 8
200.0 μl of Polyethylene glycol 400 (100 v/v)
162.5 μl of Sodium acetate (2 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
537.5 μl of water (100 v/v)
G2
  • Ammonium sulfate 0.25 M -
  • MES 0.1 M pH 5.5
  • Pentaerythritol ethoxylate (15/4 EO/OH) 35 v/v -
pH 5.5
250.0 μl of Ammonium sulfate (1 M)
100.0 μl of MES pH 5.5 (1 M)
350.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (100 v/v)
300.0 μl of water (100 v/v)
G3
  • tri-Potassium citrate 0.1 M pH 4
  • Polyethylene glycol 400 17 v/v -
pH 4
340.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of tri-Potassium citrate pH 4 (1 M)
560.0 μl of water (100 v/v)
G4
  • Sodium chloride 0.07 M -
  • tri-Sodium citrate 0.075 M pH 3.7
  • Polyethylene glycol 400 23 v/v -
pH 3.7
460.0 μl of Polyethylene glycol 400 (50 v/v)
70.0 μl of Sodium chloride (1 M)
25.6 μl of tri-Sodium citrate pH 4 (1 M)
49.4 μl of tri-Sodium citrate pH 3.5 (1 M)
395.0 μl of water (100 v/v)
G5
  • Barium chloride 0.15 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 26 v/v -
pH 7
150.0 μl of Barium chloride (1 M)
100.0 μl of HEPES pH 7 (1 M)
520.0 μl of Polyethylene glycol 400 (50 v/v)
230.0 μl of water (100 v/v)
G6
  • Ammonium formate 1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 25 v/v -
pH 6
200.0 μl of Ammonium formate (5 M)
53.0 μl of MES pH 5.5 (1 M)
47.0 μl of MES pH 6.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
G7
  • Calcium chloride 0.06 M -
  • Magnesium chloride 0.06 M -
  • CHES 0.075 M pH 9
  • Polyethylene glycol 4,000 22 w/v -
pH 9
75.0 μl of CHES pH 9 (1 M)
60.0 μl of Calcium chloride (1 M)
60.0 μl of Magnesium chloride (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
365.0 μl of water (100 v/v)
G8
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 28 v/v -
pH 6
53.0 μl of MES pH 5.5 (1 M)
47.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium chloride (1 M)
280.0 μl of Polyethylene glycol 300 (100 v/v)
520.0 μl of water (100 v/v)
G9
  • Ammonium thiocyanate 0.4 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 10 w/v -
pH 4.5
400.0 μl of Ammonium thiocyanate (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
300.0 μl of water (100 v/v)
G10
  • Sodium acetate 0.18 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 27 v/v -
  • Pentaerythritol ethoxylate (15/4 EO/OH) 12 v/v -
pH 6
53.0 μl of MES pH 5.5 (1 M)
47.0 μl of MES pH 6.5 (1 M)
240.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
270.0 μl of Polyethylene glycol 400 (100 v/v)
90.0 μl of Sodium acetate (2 M)
300.0 μl of water (100 v/v)
G11
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 10 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium chloride (2 M)
600.0 μl of water (100 v/v)
G12
  • Magnesium acetate 0.01 M -
  • Sodium acetate 0.05 M pH 5
  • Polyethylene glycol 400 23 v/v -
pH 5
10.0 μl of Magnesium acetate (1 M)
460.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Sodium acetate pH 5 (1 M)
480.0 μl of water (100 v/v)
H1
  • Barium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 300 28 v/v -
pH 8.5
100.0 μl of Barium chloride (1 M)
280.0 μl of Polyethylene glycol 300 (100 v/v)
21.2 μl of tris chloride BCC pH 7 (1 M)
78.8 μl of tris chloride BCC pH 9 (1 M)
520.0 μl of water (100 v/v)
H2
  • tri-Sodium citrate 0.05 M pH 5
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
pH 5
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of tri-Sodium citrate pH 5 (1 M)
450.0 μl of water (100 v/v)
H3
  • Sodium chloride 0.275 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 25 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
275.0 μl of Sodium chloride (1 M)
375.0 μl of water (100 v/v)
H4
  • Sodium sulfate 0.15 M -
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 400 27 v/v -
pH 7
270.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of Sodium sulfate (1 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
480.0 μl of water (100 v/v)
H5
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 1,500 22 w/v -
pH 6.5
440.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
460.0 μl of water (100 v/v)
H6
  • Cadmium chloride 0.001 M -
  • Glycine 0.1 M pH 9.5
  • 2-methyl-2,4-pentanediol BCC 60 v/v -
pH 9.5
600.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
10.0 μl of Cadmium chloride (0.1 M)
100.0 μl of Glycine pH 9.5 (1 M)
290.0 μl of water (100 v/v)
H7
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 400 27 v/v -
pH 8.5
540.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
410.0 μl of water (100 v/v)
H8
  • Potassium acetate 0.25 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 300 22 v/v -
pH 8
220.0 μl of Polyethylene glycol 300 (100 v/v)
50.0 μl of Potassium acetate (5 M)
54.7 μl of tris chloride BCC pH 7 (1 M)
45.3 μl of tris chloride BCC pH 9 (1 M)
630.0 μl of water (100 v/v)
H9
  • Magnesium chloride 0.125 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol monomethyl ether 2,000 14 w/v -
pH 6
53.0 μl of MES pH 5.5 (1 M)
47.0 μl of MES pH 6.5 (1 M)
125.0 μl of Magnesium chloride (1 M)
280.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
495.0 μl of water (100 v/v)
H10
  • Sodium chloride 0.4 M -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 600 26 v/v -
pH 8
260.0 μl of Polyethylene glycol 600 (100 v/v)
200.0 μl of Sodium chloride (2 M)
27.3 μl of tris chloride BCC pH 7 (1 M)
22.7 μl of tris chloride BCC pH 9 (1 M)
490.0 μl of water (100 v/v)
H11
  • Ammonium acetate 0.2 M -
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6
  • Polyethylene glycol 4,000 12 w/v -
pH 6
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium chloride (2 M)
410.0 μl of water (100 v/v)
H12
  • tri-Sodium citrate 0.09 M pH 5.6
  • Polyethylene glycol 400 30 v/v -
pH 5.6
600.0 μl of Polyethylene glycol 400 (50 v/v)
90.0 μl of tri-Sodium citrate pH 5.6 (1 M)
310.0 μl of water (100 v/v)