C6 - Screen Contents - RUBIC Buffer

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Screen Description

RUBIC Buffer

Molecular Dimensions / RUBIC Buffer

Created 2018-03-08.

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RUBIC Buffer

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RUBIC Buffer

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Screen Structure

RUBIC Buffer

Well Count	96
Number of distinct ingredients	18
Internal diversity (score mean dist)	0.879

Screen Composition

More information about screen composition chart.

ingredient type	RUBIC Buffer
Buffer	94
Salt	64
Water	1

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

RUBIC Buffer Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Water 100 v/v - 🔍	NA	1000.0 μl of Water (100 v/v)
A2	tri-Sodium citrate 0.119 M pH 4 🔍	pH 4	119.0 μl of tri-Sodium citrate pH 4 (1 M) 881.0 μl of water (100 v/v)
A3	Sodium acetate 0.119 M pH 4.5 🔍	pH 4.5	119.0 μl of Sodium acetate pH 4.5 (1 M) 881.0 μl of water (100 v/v)
A4	tri-Sodium citrate 0.119 M pH 5 🔍	pH 5	119.0 μl of tri-Sodium citrate pH 5 (1 M) 881.0 μl of water (100 v/v)
A5	MES 0.119 M pH 6 🔍	pH 6	119.0 μl of MES pH 6 (1 M) 881.0 μl of water (100 v/v)
A6	Potassium di-hydrogen phosphate 0.119 M pH 6 🔍	pH 6	54.4 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 5.1 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M) 940.5 μl of water (100 v/v)
A7	tri-Sodium citrate 0.119 M pH 6 🔍	pH 6	26.8 μl of tri-Sodium citrate pH 5.8 (1 M) 92.2 μl of tri-Sodium citrate pH 7 (1 M) 881.0 μl of water (100 v/v)
A8	BIS-TRIS 0.119 M pH 6.5 🔍	pH 6.5	119.0 μl of BIS-TRIS pH 6.5 (1 M) 881.0 μl of water (100 v/v)
A9	MES 0.119 M pH 6.5 🔍	pH 6.5	119.0 μl of MES pH 6.5 (1 M) 881.0 μl of water (100 v/v)
A10	Sodium di-hydrogen phosphate 0.119 M pH 7 🔍	pH 7	39.7 μl of Sodium di-hydrogen phosphate pH 7 (3 M) 960.3 μl of water (100 v/v)
A11	di-Potassium hydrogen phosphate 0.119 M pH 7 🔍	pH 7	59.5 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 940.5 μl of water (100 v/v)
A12	HEPES 0.119 M pH 7 🔍	pH 7	119.0 μl of HEPES pH 7 (1 M) 881.0 μl of water (100 v/v)
B1	MOPS 0.119 M pH 7 🔍	pH 7	119.0 μl of MOPS pH 7 (1 M) 881.0 μl of water (100 v/v)
B2	Ammonium acetate 0.119 M pH 7.3 🔍	pH 7.3	0.2 μl of Ammonium acetate pH 7 (1 M) 118.8 μl of Ammonium acetate pH 10 (1 M) 881.0 μl of water (100 v/v)
B3	tris chloride BCC 0.119 M pH 7.5 🔍	pH 7.5	119.0 μl of tris chloride BCC pH 7.5 (1 M) 881.0 μl of water (100 v/v)
B4	Sodium di-hydrogen phosphate 0.119 M pH 7.5 🔍	pH 7.5	39.7 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 960.3 μl of water (100 v/v)
B5	Imidazole 0.119 M pH 7.5 🔍	pH 7.5	119.0 μl of Imidazole pH 7.5 (1 M) 881.0 μl of water (100 v/v)
B6	HEPES 0.119 M pH 8 🔍	pH 8	119.0 μl of HEPES pH 8 (1 M) 881.0 μl of water (100 v/v)
B7	tris chloride BCC 0.119 M pH 8 🔍	pH 8	119.0 μl of tris chloride BCC pH 8 (1 M) 881.0 μl of water (100 v/v)
B8	TRICINE 0.119 M pH 8 🔍	pH 8	119.0 μl of TRICINE pH 8 (1 M) 881.0 μl of water (100 v/v)
B9	BICINE 0.119 M pH 8 🔍	pH 8	119.0 μl of BICINE pH 8 (1 M) 881.0 μl of water (100 v/v)
B10	BICINE 0.119 M pH 8.5 🔍	pH 8.5	119.0 μl of BICINE pH 8.5 (1 M) 881.0 μl of water (100 v/v)
B11	tris chloride BCC 0.119 M pH 8.5 🔍	pH 8.5	119.0 μl of tris chloride BCC pH 8.5 (1 M) 881.0 μl of water (100 v/v)
B12	CHES 0.119 M pH 9 🔍	pH 9	119.0 μl of CHES pH 9 (1 M) 881.0 μl of water (100 v/v)
C1	Sodium chloride 0.298 M - 🔍	NA	298.0 μl of Sodium chloride (1 M) 702.0 μl of water (100 v/v)
C2	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 4 🔍	pH 4	298.0 μl of Sodium chloride (1 M) 119.0 μl of tri-Sodium citrate pH 4 (1 M) 583.0 μl of water (100 v/v)
C3	Sodium chloride 0.298 M - Sodium acetate 0.119 M pH 4.5 🔍	pH 4.5	119.0 μl of Sodium acetate pH 4.5 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
C4	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 5 🔍	pH 5	298.0 μl of Sodium chloride (1 M) 119.0 μl of tri-Sodium citrate pH 5 (1 M) 583.0 μl of water (100 v/v)
C5	Sodium chloride 0.298 M - MES 0.119 M pH 6 🔍	pH 6	119.0 μl of MES pH 6 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
C6	Sodium chloride 0.298 M - Potassium di-hydrogen phosphate 0.119 M pH 6 🔍	pH 6	54.4 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 5.1 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M) 298.0 μl of Sodium chloride (1 M) 642.5 μl of water (100 v/v)
C7	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 6 🔍	pH 6	298.0 μl of Sodium chloride (1 M) 26.8 μl of tri-Sodium citrate pH 5.8 (1 M) 92.2 μl of tri-Sodium citrate pH 7 (1 M) 583.0 μl of water (100 v/v)
C8	Sodium chloride 0.298 M - BIS-TRIS 0.119 M pH 6.5 🔍	pH 6.5	119.0 μl of BIS-TRIS pH 6.5 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
C9	Sodium chloride 0.298 M - MES 0.119 M pH 6.5 🔍	pH 6.5	119.0 μl of MES pH 6.5 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
C10	Sodium chloride 0.298 M - Sodium di-hydrogen phosphate 0.119 M pH 7 🔍	pH 7	298.0 μl of Sodium chloride (1 M) 39.7 μl of Sodium di-hydrogen phosphate pH 7 (3 M) 662.3 μl of water (100 v/v)
C11	Sodium chloride 0.298 M - di-Potassium hydrogen phosphate 0.119 M pH 7 🔍	pH 7	298.0 μl of Sodium chloride (1 M) 59.5 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 642.5 μl of water (100 v/v)
C12	Sodium chloride 0.298 M - HEPES 0.119 M pH 7 🔍	pH 7	119.0 μl of HEPES pH 7 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D1	Sodium chloride 0.298 M - MOPS 0.119 M pH 7 🔍	pH 7	119.0 μl of MOPS pH 7 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D2	Sodium chloride 0.298 M - Ammonium acetate 0.119 M pH 7.3 🔍	pH 7.3	0.2 μl of Ammonium acetate pH 7 (1 M) 118.8 μl of Ammonium acetate pH 10 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D3	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 7.5 🔍	pH 7.5	298.0 μl of Sodium chloride (1 M) 119.0 μl of tris chloride BCC pH 7.5 (1 M) 583.0 μl of water (100 v/v)
D4	Sodium di-hydrogen phosphate 0.119 M pH 7.5 Sodium chloride 0.298 M - 🔍	pH 7.5	298.0 μl of Sodium chloride (1 M) 39.7 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 662.3 μl of water (100 v/v)
D5	Sodium chloride 0.298 M - Imidazole 0.119 M pH 7.5 🔍	pH 7.5	119.0 μl of Imidazole pH 7.5 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D6	Sodium chloride 0.298 M - HEPES 0.119 M pH 8 🔍	pH 8	119.0 μl of HEPES pH 8 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D7	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 8 🔍	pH 8	298.0 μl of Sodium chloride (1 M) 119.0 μl of tris chloride BCC pH 8 (1 M) 583.0 μl of water (100 v/v)
D8	Sodium chloride 0.298 M - TRICINE 0.119 M pH 8 🔍	pH 8	298.0 μl of Sodium chloride (1 M) 119.0 μl of TRICINE pH 8 (1 M) 583.0 μl of water (100 v/v)
D9	Sodium chloride 0.298 M - BICINE 0.119 M pH 8 🔍	pH 8	119.0 μl of BICINE pH 8 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D10	Sodium chloride 0.298 M - BICINE 0.119 M pH 8.5 🔍	pH 8.5	119.0 μl of BICINE pH 8.5 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
D11	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 8.5 🔍	pH 8.5	298.0 μl of Sodium chloride (1 M) 119.0 μl of tris chloride BCC pH 8.5 (1 M) 583.0 μl of water (100 v/v)
D12	Sodium chloride 0.298 M - CHES 0.119 M pH 9 🔍	pH 9	119.0 μl of CHES pH 9 (1 M) 298.0 μl of Sodium chloride (1 M) 583.0 μl of water (100 v/v)
E1	SPG buffer 0.119 M pH 4 🔍	pH 4	119.0 μl of SPG buffer pH 4 (1 M) 881.0 μl of water (100 v/v)
E2	SPG buffer 0.119 M pH 4.5 🔍	pH 4.5	108.2 μl of SPG buffer pH 4 (1 M) 10.8 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E3	SPG buffer 0.119 M pH 5 🔍	pH 5	108.2 μl of SPG buffer pH 4 (1 M) 10.8 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E4	SPG buffer 0.119 M pH 5.5 🔍	pH 5.5	108.2 μl of SPG buffer pH 4 (1 M) 10.8 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E5	SPG buffer 0.119 M pH 6 🔍	pH 6	108.2 μl of SPG buffer pH 4 (1 M) 10.8 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E6	SPG buffer 0.119 M pH 6.5 🔍	pH 6.5	108.2 μl of SPG buffer pH 4 (1 M) 10.8 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E7	SPG buffer 0.119 M pH 7 🔍	pH 7	2.3 μl of SPG buffer pH 4 (1 M) 116.7 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E8	SPG buffer 0.119 M pH 7.5 🔍	pH 7.5	2.3 μl of SPG buffer pH 4 (1 M) 116.7 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E9	SPG buffer 0.119 M pH 8 🔍	pH 8	2.3 μl of SPG buffer pH 4 (1 M) 116.7 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E10	SPG buffer 0.119 M pH 8.5 🔍	pH 8.5	2.3 μl of SPG buffer pH 4 (1 M) 116.7 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E11	SPG buffer 0.119 M pH 9 🔍	pH 9	2.3 μl of SPG buffer pH 4 (1 M) 116.7 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
E12	SPG buffer 0.119 M pH 10 🔍	pH 10	119.0 μl of SPG buffer pH 10 (1 M) 881.0 μl of water (100 v/v)
F1	HEPES 0.024 M pH 7.5 🔍	pH 7.5	24.0 μl of HEPES pH 7.5 (1 M) 976.0 μl of water (100 v/v)
F2	HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 940.0 μl of water (100 v/v)
F3	HEPES 0.149 M pH 7.5 🔍	pH 7.5	149.0 μl of HEPES pH 7.5 (1 M) 851.0 μl of water (100 v/v)
F4	HEPES 0.298 M pH 7.5 🔍	pH 7.5	298.0 μl of HEPES pH 7.5 (1 M) 702.0 μl of water (100 v/v)
F5	Sodium di-hydrogen phosphate 0.024 M pH 7.5 🔍	pH 7.5	8.0 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 992.0 μl of water (100 v/v)
F6	Sodium di-hydrogen phosphate 0.06 M pH 7.5 🔍	pH 7.5	20.0 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 980.0 μl of water (100 v/v)
F7	Sodium di-hydrogen phosphate 0.149 M pH 7.5 🔍	pH 7.5	49.7 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 950.3 μl of water (100 v/v)
F8	Sodium di-hydrogen phosphate 0.298 M pH 7.5 🔍	pH 7.5	99.3 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 900.7 μl of water (100 v/v)
F9	tris chloride BCC 0.024 M pH 8 🔍	pH 8	24.0 μl of tris chloride BCC pH 8 (1 M) 976.0 μl of water (100 v/v)
F10	tris chloride BCC 0.06 M pH 8 🔍	pH 8	60.0 μl of tris chloride BCC pH 8 (1 M) 940.0 μl of water (100 v/v)
F11	tris chloride BCC 0.149 M pH 8 🔍	pH 8	149.0 μl of tris chloride BCC pH 8 (1 M) 851.0 μl of water (100 v/v)
F12	tris chloride BCC 0.298 M pH 8 🔍	pH 8	298.0 μl of tris chloride BCC pH 8 (1 M) 702.0 μl of water (100 v/v)
G1	Sodium chloride 0.06 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 60.0 μl of Sodium chloride (1 M) 880.0 μl of water (100 v/v)
G2	Sodium chloride 0.149 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 149.0 μl of Sodium chloride (1 M) 791.0 μl of water (100 v/v)
G3	Sodium chloride 0.298 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 298.0 μl of Sodium chloride (1 M) 642.0 μl of water (100 v/v)
G4	Sodium chloride 0.595 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 595.0 μl of Sodium chloride (1 M) 345.0 μl of water (100 v/v)
G5	Sodium chloride 0.893 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 178.6 μl of Sodium chloride (5 M) 761.4 μl of water (100 v/v)
G6	Sodium chloride 1.19 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of HEPES pH 7.5 (1 M) 238.0 μl of Sodium chloride (5 M) 702.0 μl of water (100 v/v)
G7	Sodium chloride 0.06 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	12.0 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 928.0 μl of water (100 v/v)
G8	Sodium chloride 0.149 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	29.8 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 910.2 μl of water (100 v/v)
G9	Sodium chloride 0.298 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	59.6 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 880.4 μl of water (100 v/v)
G10	Sodium chloride 0.595 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	119.0 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 821.0 μl of water (100 v/v)
G11	Sodium chloride 0.893 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	178.6 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 761.4 μl of water (100 v/v)
G12	Sodium chloride 1.19 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	238.0 μl of Sodium chloride (5 M) 60.0 μl of tris chloride BCC pH 8 (1 M) 702.0 μl of water (100 v/v)
H1	BIS-TRIS 0.06 M pH 6 🔍	pH 6	60.0 μl of BIS-TRIS pH 6 (1 M) 940.0 μl of water (100 v/v)
H2	Imidazole 0.06 M pH 6.5 🔍	pH 6.5	60.0 μl of Imidazole pH 6.5 (1 M) 940.0 μl of water (100 v/v)
H3	BIS-TRIS 0.06 M pH 6.5 🔍	pH 6.5	60.0 μl of BIS-TRIS pH 6.5 (1 M) 940.0 μl of water (100 v/v)
H4	MOPS 0.06 M pH 7 🔍	pH 7	60.0 μl of MOPS pH 7 (1 M) 940.0 μl of water (100 v/v)
H5	tri-Sodium citrate 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of tri-Sodium citrate pH 7.5 (1 M) 940.0 μl of water (100 v/v)
H6	MOPS 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of MOPS pH 7.5 (1 M) 940.0 μl of water (100 v/v)
H7	tris chloride BCC 0.06 M pH 8.5 🔍	pH 8.5	60.0 μl of tris chloride BCC pH 8.5 (1 M) 940.0 μl of water (100 v/v)
H8	Sodium chloride 0.119 M - Imidazole 0.06 M pH 7.5 🔍	pH 7.5	60.0 μl of Imidazole pH 7.5 (1 M) 23.8 μl of Sodium chloride (5 M) 916.2 μl of water (100 v/v)
H9	Sodium chloride 0.119 M - Imidazole 0.149 M pH 7.5 🔍	pH 7.5	149.0 μl of Imidazole pH 7.5 (1 M) 23.8 μl of Sodium chloride (5 M) 827.2 μl of water (100 v/v)
H10	Sodium chloride 0.119 M - Imidazole 0.298 M pH 7.5 🔍	pH 7.5	298.0 μl of Imidazole pH 7.5 (1 M) 23.8 μl of Sodium chloride (5 M) 678.2 μl of water (100 v/v)
H11	Sodium chloride 0.119 M - Imidazole 0.417 M pH 7.5 🔍	pH 7.5	417.0 μl of Imidazole pH 7.5 (1 M) 23.8 μl of Sodium chloride (5 M) 559.2 μl of water (100 v/v)
H12	Sodium chloride 0.119 M - Imidazole 0.595 M pH 7.5 🔍	pH 7.5	595.0 μl of Imidazole pH 7.5 (1 M) 23.8 μl of Sodium chloride (5 M) 381.2 μl of water (100 v/v)