C6 - Screen Contents - RUBIC Buffer

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Supplemental Information

Screen Description

RUBIC Buffer

Molecular Dimensions / RUBIC Buffer

Created 2018-03-08.

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RUBIC Buffer

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RUBIC Buffer

Screen Structure

RUBIC Buffer

Well Count	96
Number of distinct ingredients	18
Internal diversity (score mean dist)	0.879

Screen Composition

More information about screen composition chart.

ingredient type	RUBIC Buffer
Buffer	94
Salt	64
Water	1

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

RUBIC Buffer Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Water 100 v/v - 🔍	NA	1000 μl of Water (100 v/v)
A2	tri-Sodium citrate 0.119 M pH 4 🔍	pH 4	119 μl of tri-Sodium citrate pH 4 (1 M) 881 μl of water (100 v/v)
A3	Sodium acetate 0.119 M pH 4.5 🔍	pH 4.5	119 μl of Sodium acetate pH 4.5 (1 M) 881 μl of water (100 v/v)
A4	tri-Sodium citrate 0.119 M pH 5 🔍	pH 5	119 μl of tri-Sodium citrate pH 5 (1 M) 881 μl of water (100 v/v)
A5	MES 0.119 M pH 6 🔍	pH 6	119 μl of MES pH 6 (1 M) 881 μl of water (100 v/v)
A6	Potassium di-hydrogen phosphate 0.119 M pH 6 🔍	pH 6	54 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 5 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M) 940 μl of water (100 v/v)
A7	tri-Sodium citrate 0.119 M pH 6 🔍	pH 6	3 μl of tri-Sodium citrate pH 5.8 (1 M) 115 μl of tri-Sodium citrate pH 7 (1 M) 881 μl of water (100 v/v)
A8	BIS-TRIS 0.119 M pH 6.5 🔍	pH 6.5	119 μl of BIS-TRIS pH 6.5 (1 M) 881 μl of water (100 v/v)
A9	MES 0.119 M pH 6.5 🔍	pH 6.5	119 μl of MES pH 6.5 (1 M) 881 μl of water (100 v/v)
A10	Sodium di-hydrogen phosphate 0.119 M pH 7 🔍	pH 7	39 μl of Sodium di-hydrogen phosphate pH 7 (3 M) 960 μl of water (100 v/v)
A11	di-Potassium hydrogen phosphate 0.119 M pH 7 🔍	pH 7	59 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 940 μl of water (100 v/v)
A12	HEPES 0.119 M pH 7 🔍	pH 7	119 μl of HEPES pH 7 (1 M) 881 μl of water (100 v/v)
B1	MOPS 0.119 M pH 7 🔍	pH 7	119 μl of MOPS pH 7 (1 M) 881 μl of water (100 v/v)
B2	Ammonium acetate 0.119 M pH 7.3 🔍	pH 7.3	0 μl of Ammonium acetate pH 7 (1 M) 119 μl of Ammonium acetate pH 10 (1 M) 881 μl of water (100 v/v)
B3	tris chloride BCC 0.119 M pH 7.5 🔍	pH 7.5	119 μl of tris chloride BCC pH 7.5 (1 M) 881 μl of water (100 v/v)
B4	Sodium di-hydrogen phosphate 0.119 M pH 7.5 🔍	pH 7.5	39 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 960 μl of water (100 v/v)
B5	Imidazole 0.119 M pH 7.5 🔍	pH 7.5	119 μl of Imidazole pH 7.5 (1 M) 881 μl of water (100 v/v)
B6	HEPES 0.119 M pH 8 🔍	pH 8	119 μl of HEPES pH 8 (1 M) 881 μl of water (100 v/v)
B7	tris chloride BCC 0.119 M pH 8 🔍	pH 8	119 μl of tris chloride BCC pH 8 (1 M) 881 μl of water (100 v/v)
B8	TRICINE 0.119 M pH 8 🔍	pH 8	119 μl of TRICINE pH 8 (1 M) 881 μl of water (100 v/v)
B9	BICINE 0.119 M pH 8 🔍	pH 8	119 μl of BICINE pH 8 (1 M) 881 μl of water (100 v/v)
B10	BICINE 0.119 M pH 8.5 🔍	pH 8.5	119 μl of BICINE pH 8.5 (1 M) 881 μl of water (100 v/v)
B11	tris chloride BCC 0.119 M pH 8.5 🔍	pH 8.5	119 μl of tris chloride BCC pH 8.5 (1 M) 881 μl of water (100 v/v)
B12	CHES 0.119 M pH 9 🔍	pH 9	119 μl of CHES pH 9 (1 M) 881 μl of water (100 v/v)
C1	Sodium chloride 0.298 M - 🔍	NA	298 μl of Sodium chloride (1 M) 702 μl of water (100 v/v)
C2	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 4 🔍	pH 4	298 μl of Sodium chloride (1 M) 119 μl of tri-Sodium citrate pH 4 (1 M) 583 μl of water (100 v/v)
C3	Sodium chloride 0.298 M - Sodium acetate 0.119 M pH 4.5 🔍	pH 4.5	119 μl of Sodium acetate pH 4.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C4	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 5 🔍	pH 5	119 μl of tri-Sodium citrate pH 5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C5	Sodium chloride 0.298 M - MES 0.119 M pH 6 🔍	pH 6	119 μl of MES pH 6 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C6	Sodium chloride 0.298 M - Potassium di-hydrogen phosphate 0.119 M pH 6 🔍	pH 6	54 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M) 5 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M) 298 μl of Sodium chloride (1 M) 642 μl of water (100 v/v)
C7	Sodium chloride 0.298 M - tri-Sodium citrate 0.119 M pH 6 🔍	pH 6	3 μl of tri-Sodium citrate pH 5.8 (1 M) 115 μl of tri-Sodium citrate pH 7 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C8	Sodium chloride 0.298 M - BIS-TRIS 0.119 M pH 6.5 🔍	pH 6.5	119 μl of BIS-TRIS pH 6.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C9	Sodium chloride 0.298 M - MES 0.119 M pH 6.5 🔍	pH 6.5	119 μl of MES pH 6.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
C10	Sodium chloride 0.298 M - Sodium di-hydrogen phosphate 0.119 M pH 7 🔍	pH 7	39 μl of Sodium di-hydrogen phosphate pH 7 (3 M) 298 μl of Sodium chloride (1 M) 662 μl of water (100 v/v)
C11	Sodium chloride 0.298 M - di-Potassium hydrogen phosphate 0.119 M pH 7 🔍	pH 7	59 μl of di-Potassium hydrogen phosphate pH 7 (2 M) 298 μl of Sodium chloride (1 M) 642 μl of water (100 v/v)
C12	Sodium chloride 0.298 M - HEPES 0.119 M pH 7 🔍	pH 7	119 μl of HEPES pH 7 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D1	Sodium chloride 0.298 M - MOPS 0.119 M pH 7 🔍	pH 7	119 μl of MOPS pH 7 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D2	Sodium chloride 0.298 M - Ammonium acetate 0.119 M pH 7.3 🔍	pH 7.3	0 μl of Ammonium acetate pH 7 (1 M) 119 μl of Ammonium acetate pH 10 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D3	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 7.5 🔍	pH 7.5	119 μl of tris chloride BCC pH 7.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D4	Sodium di-hydrogen phosphate 0.119 M pH 7.5 Sodium chloride 0.298 M - 🔍	pH 7.5	39 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 298 μl of Sodium chloride (1 M) 662 μl of water (100 v/v)
D5	Sodium chloride 0.298 M - Imidazole 0.119 M pH 7.5 🔍	pH 7.5	119 μl of Imidazole pH 7.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D6	Sodium chloride 0.298 M - HEPES 0.119 M pH 8 🔍	pH 8	119 μl of HEPES pH 8 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D7	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 8 🔍	pH 8	119 μl of tris chloride BCC pH 8 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D8	Sodium chloride 0.298 M - TRICINE 0.119 M pH 8 🔍	pH 8	119 μl of TRICINE pH 8 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D9	Sodium chloride 0.298 M - BICINE 0.119 M pH 8 🔍	pH 8	119 μl of BICINE pH 8 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D10	Sodium chloride 0.298 M - BICINE 0.119 M pH 8.5 🔍	pH 8.5	119 μl of BICINE pH 8.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D11	Sodium chloride 0.298 M - tris chloride BCC 0.119 M pH 8.5 🔍	pH 8.5	119 μl of tris chloride BCC pH 8.5 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
D12	Sodium chloride 0.298 M - CHES 0.119 M pH 9 🔍	pH 9	119 μl of CHES pH 9 (1 M) 298 μl of Sodium chloride (1 M) 583 μl of water (100 v/v)
E1	SPG buffer 0.119 M pH 4 🔍	pH 4	119 μl of SPG buffer pH 4 (1 M) 881 μl of water (100 v/v)
E2	SPG buffer 0.119 M pH 4.5 🔍	pH 4.5	119 μl of SPG buffer pH 4 (1 M) 0 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E3	SPG buffer 0.119 M pH 5 🔍	pH 5	119 μl of SPG buffer pH 4 (1 M) 0 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E4	SPG buffer 0.119 M pH 5.5 🔍	pH 5.5	119 μl of SPG buffer pH 4 (1 M) 0 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E5	SPG buffer 0.119 M pH 6 🔍	pH 6	119 μl of SPG buffer pH 4 (1 M) 0 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E6	SPG buffer 0.119 M pH 6.5 🔍	pH 6.5	119 μl of SPG buffer pH 4 (1 M) 0 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E7	SPG buffer 0.119 M pH 7 🔍	pH 7	0 μl of SPG buffer pH 4 (1 M) 118 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E8	SPG buffer 0.119 M pH 7.5 🔍	pH 7.5	0 μl of SPG buffer pH 4 (1 M) 118 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E9	SPG buffer 0.119 M pH 8 🔍	pH 8	0 μl of SPG buffer pH 4 (1 M) 118 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E10	SPG buffer 0.119 M pH 8.5 🔍	pH 8.5	0 μl of SPG buffer pH 4 (1 M) 118 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E11	SPG buffer 0.119 M pH 9 🔍	pH 9	0 μl of SPG buffer pH 4 (1 M) 118 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
E12	SPG buffer 0.119 M pH 10 🔍	pH 10	119 μl of SPG buffer pH 10 (1 M) 881 μl of water (100 v/v)
F1	HEPES 0.024 M pH 7.5 🔍	pH 7.5	24 μl of HEPES pH 7.5 (1 M) 976 μl of water (100 v/v)
F2	HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 940 μl of water (100 v/v)
F3	HEPES 0.149 M pH 7.5 🔍	pH 7.5	149 μl of HEPES pH 7.5 (1 M) 851 μl of water (100 v/v)
F4	HEPES 0.298 M pH 7.5 🔍	pH 7.5	298 μl of HEPES pH 7.5 (1 M) 702 μl of water (100 v/v)
F5	Sodium di-hydrogen phosphate 0.024 M pH 7.5 🔍	pH 7.5	8 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 992 μl of water (100 v/v)
F6	Sodium di-hydrogen phosphate 0.06 M pH 7.5 🔍	pH 7.5	20 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 980 μl of water (100 v/v)
F7	Sodium di-hydrogen phosphate 0.149 M pH 7.5 🔍	pH 7.5	49 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 950 μl of water (100 v/v)
F8	Sodium di-hydrogen phosphate 0.298 M pH 7.5 🔍	pH 7.5	99 μl of Sodium di-hydrogen phosphate pH 7.5 (3 M) 900 μl of water (100 v/v)
F9	tris chloride BCC 0.024 M pH 8 🔍	pH 8	24 μl of tris chloride BCC pH 8 (1 M) 976 μl of water (100 v/v)
F10	tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 940 μl of water (100 v/v)
F11	tris chloride BCC 0.149 M pH 8 🔍	pH 8	149 μl of tris chloride BCC pH 8 (1 M) 851 μl of water (100 v/v)
F12	tris chloride BCC 0.298 M pH 8 🔍	pH 8	298 μl of tris chloride BCC pH 8 (1 M) 702 μl of water (100 v/v)
G1	Sodium chloride 0.06 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 60 μl of Sodium chloride (1 M) 880 μl of water (100 v/v)
G2	Sodium chloride 0.149 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 149 μl of Sodium chloride (1 M) 791 μl of water (100 v/v)
G3	Sodium chloride 0.298 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 298 μl of Sodium chloride (1 M) 642 μl of water (100 v/v)
G4	Sodium chloride 0.595 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 595 μl of Sodium chloride (1 M) 345 μl of water (100 v/v)
G5	Sodium chloride 0.893 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 178 μl of Sodium chloride (5 M) 761 μl of water (100 v/v)
G6	Sodium chloride 1.19 M - HEPES 0.06 M pH 7.5 🔍	pH 7.5	60 μl of HEPES pH 7.5 (1 M) 238 μl of Sodium chloride (5 M) 702 μl of water (100 v/v)
G7	Sodium chloride 0.06 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 12 μl of Sodium chloride (5 M) 928 μl of water (100 v/v)
G8	Sodium chloride 0.149 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 29 μl of Sodium chloride (5 M) 910 μl of water (100 v/v)
G9	Sodium chloride 0.298 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 59 μl of Sodium chloride (5 M) 880 μl of water (100 v/v)
G10	Sodium chloride 0.595 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 119 μl of Sodium chloride (5 M) 821 μl of water (100 v/v)
G11	Sodium chloride 0.893 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 178 μl of Sodium chloride (5 M) 761 μl of water (100 v/v)
G12	Sodium chloride 1.19 M - tris chloride BCC 0.06 M pH 8 🔍	pH 8	60 μl of tris chloride BCC pH 8 (1 M) 238 μl of Sodium chloride (5 M) 702 μl of water (100 v/v)
H1	BIS-TRIS 0.06 M pH 6 🔍	pH 6	60 μl of BIS-TRIS pH 6 (1 M) 940 μl of water (100 v/v)
H2	Imidazole 0.06 M pH 6.5 🔍	pH 6.5	60 μl of Imidazole pH 6.5 (1 M) 940 μl of water (100 v/v)
H3	BIS-TRIS 0.06 M pH 6.5 🔍	pH 6.5	60 μl of BIS-TRIS pH 6.5 (1 M) 940 μl of water (100 v/v)
H4	MOPS 0.06 M pH 7 🔍	pH 7	60 μl of MOPS pH 7 (1 M) 940 μl of water (100 v/v)
H5	tri-Sodium citrate 0.06 M pH 7.5 🔍	pH 7.5	60 μl of tri-Sodium citrate pH 7.5 (1 M) 940 μl of water (100 v/v)
H6	MOPS 0.06 M pH 7.5 🔍	pH 7.5	60 μl of MOPS pH 7.5 (1 M) 940 μl of water (100 v/v)
H7	tris chloride BCC 0.06 M pH 8.5 🔍	pH 8.5	60 μl of tris chloride BCC pH 8.5 (1 M) 940 μl of water (100 v/v)
H8	Sodium chloride 0.119 M - Imidazole 0.06 M pH 7.5 🔍	pH 7.5	60 μl of Imidazole pH 7.5 (1 M) 23 μl of Sodium chloride (5 M) 916 μl of water (100 v/v)
H9	Sodium chloride 0.119 M - Imidazole 0.149 M pH 7.5 🔍	pH 7.5	149 μl of Imidazole pH 7.5 (1 M) 23 μl of Sodium chloride (5 M) 827 μl of water (100 v/v)
H10	Sodium chloride 0.119 M - Imidazole 0.298 M pH 7.5 🔍	pH 7.5	298 μl of Imidazole pH 7.5 (1 M) 23 μl of Sodium chloride (5 M) 678 μl of water (100 v/v)
H11	Sodium chloride 0.119 M - Imidazole 0.417 M pH 7.5 🔍	pH 7.5	417 μl of Imidazole pH 7.5 (1 M) 23 μl of Sodium chloride (5 M) 559 μl of water (100 v/v)
H12	Sodium chloride 0.119 M - Imidazole 0.595 M pH 7.5 🔍	pH 7.5	595 μl of Imidazole pH 7.5 (1 M) 23 μl of Sodium chloride (5 M) 381 μl of water (100 v/v)