C6 - Screen Contents - Protein-Nucleic Acid Complex Crystal

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Supplemental Information

Screen Description

Protein-Nucleic Acid Complex Crystal

Kerafast / Protein-Nucleic Acid Complex Crystal

Created 2018-03-08.

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Protein-Nucleic Acid Complex Crystal

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Protein-Nucleic Acid Complex Crystal

Screen Structure

Protein-Nucleic Acid Complex Crystal

Well Count	48
Number of distinct ingredients	9
Internal diversity (score mean dist)	0.753

Screen Composition

More information about screen composition chart.

ingredient type	Protein-Nucleic Acid Complex Crystal
Buffer	48
Polymer	48
Salt	78

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Protein-Nucleic Acid Complex Crystal Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 6 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	120 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 780 μl of water (100 v/v)
A2	Polyethylene glycol 8,000 16 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50 μl of Calcium chloride (2 M) 100 μl of MES pH 6 (1 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 480 μl of water (100 v/v)
A3	Polyethylene glycol 4,000 11 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.05 M - 🔍	pH 7.5	25 μl of Magnesium chloride (2 M) 220 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 655 μl of water (100 v/v)
A4	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.05 M - 🔍	pH 4.6	25 μl of Calcium chloride (2 M) 120 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 755 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.05 M - Sodium chloride 0.1 M - 🔍	pH 7.5	25 μl of Magnesium chloride (2 M) 320 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 505 μl of water (100 v/v)
A7	Polyethylene glycol 2,000 11 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - 🔍	pH 6	220 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 630 μl of water (100 v/v)
A8	Polyethylene glycol 2,000 20 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 425 μl of water (100 v/v)
B1	Polyethylene glycol 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50 μl of Calcium chloride (2 M) 320 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 480 μl of water (100 v/v)
B2	Polyethylene glycol 2,000 25 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 500 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 300 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 20 w/v - MES 0.1 M pH 6 Magnesium chloride 0.1 M - 🔍	pH 6	50 μl of Magnesium chloride (2 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 450 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 11 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	220 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 680 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 25 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50 μl of Calcium chloride (2 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 300 μl of water (100 v/v)
B6	Polyethylene glycol 2,000 20 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - 🔍	pH 7.5	400 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 450 μl of water (100 v/v)
B7	Polyethylene glycol 8,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.1 M - 🔍	pH 4.6	50 μl of Calcium chloride (2 M) 220 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 630 μl of water (100 v/v)
B8	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 🔍	pH 6	120 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 780 μl of water (100 v/v)
C1	Polyethylene glycol 2,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Magnesium chloride 0.05 M - 🔍	pH 4.6	25 μl of Magnesium chloride (2 M) 220 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 605 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	400 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 450 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 16 w/v - MES 0.1 M pH 6 Magnesium chloride 0.05 M - 🔍	pH 6	25 μl of Magnesium chloride (2 M) 320 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 555 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 6 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50 μl of Calcium chloride (2 M) 100 μl of MES pH 6 (1 M) 120 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 680 μl of water (100 v/v)
C5	Polyethylene glycol 8,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Magnesium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 4.6	50 μl of Magnesium chloride (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 11 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 220 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 630 μl of water (100 v/v)
C7	Polyethylene glycol 2,000 16 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	320 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 580 μl of water (100 v/v)
C8	Polyethylene glycol 4,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	120 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 730 μl of water (100 v/v)
D1	Polyethylene glycol 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 4.6	25 μl of Calcium chloride (2 M) 320 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 505 μl of water (100 v/v)
D2	Polyethylene glycol 4,000 20 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 425 μl of water (100 v/v)
D3	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50 μl of Calcium chloride (2 M) 120 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 680 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 11 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 220 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 630 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 475 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 320 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 530 μl of water (100 v/v)
D7	Polyethylene glycol 8,000 11 w/v - MES 0.1 M pH 6 🔍	pH 6	100 μl of MES pH 6 (1 M) 220 μl of Polyethylene glycol 8,000 (50 w/v) 680 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 6 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	120 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 780 μl of water (100 v/v)
E1	Polyethylene glycol 8,000 16 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	pH 6	50 μl of Magnesium chloride (2 M) 100 μl of MES pH 6 (1 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 480 μl of water (100 v/v)
E2	Polyethylene glycol 2,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 400 μl of water (100 v/v)
E3	Polyethylene glycol 4,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	220 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 630 μl of water (100 v/v)
E4	Polyethylene glycol 4,000 6 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25 μl of Calcium chloride (2 M) 120 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of MES pH 6 (1 M) 50 μl of Sodium chloride (2 M) 705 μl of water (100 v/v)
E5	Polyethylene glycol 4,000 6 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25 μl of Calcium chloride (2 M) 120 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 755 μl of water (100 v/v)
E6	Polyethylene glycol 8,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.05 M - Sodium chloride 0.1 M - 🔍	pH 4.6	25 μl of Calcium chloride (2 M) 320 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 505 μl of water (100 v/v)
E7	Polyethylene glycol 2,000 20 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - 🔍	pH 6	50 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 450 μl of water (100 v/v)
E8	Polyethylene glycol 2,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	pH 4.6	50 μl of Magnesium chloride (2 M) 220 μl of Polyethylene glycol 2,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 580 μl of water (100 v/v)
F1	Polyethylene glycol 4,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50 μl of Calcium chloride (2 M) 220 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of Sodium acetate pH 4.6 (1 M) 580 μl of water (100 v/v)
F2	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 🔍	pH 6	120 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of MES pH 6 (1 M) 780 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 400 μl of water (100 v/v)
F4	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - Magnesium chloride 0.05 M - 🔍	pH 7.5	25 μl of Magnesium chloride (2 M) 320 μl of Polyethylene glycol 4,000 (50 w/v) 50 μl of Sodium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 505 μl of water (100 v/v)
F5	Polyethylene glycol 2,000 6 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25 μl of Calcium chloride (2 M) 120 μl of Polyethylene glycol 2,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 755 μl of water (100 v/v)
F6	Polyethylene glycol 8,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Magnesium chloride 0.05 M - 🔍	pH 4.6	25 μl of Magnesium chloride (2 M) 120 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of Sodium acetate pH 4.6 (1 M) 755 μl of water (100 v/v)
F7	Polyethylene glycol 8,000 11 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25 μl of Calcium chloride (2 M) 100 μl of MES pH 6 (1 M) 220 μl of Polyethylene glycol 8,000 (50 w/v) 50 μl of Sodium chloride (2 M) 605 μl of water (100 v/v)
F8	Polyethylene glycol 4,000 25 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50 μl of Calcium chloride (2 M) 500 μl of Polyethylene glycol 4,000 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 350 μl of water (100 v/v)