C6 - Screen Contents - Protein-Nucleic Acid Complex Crystal

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Supplemental Information

Screen Description

Protein-Nucleic Acid Complex Crystal

Kerafast / Protein-Nucleic Acid Complex Crystal

Created 2018-03-08.

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Protein-Nucleic Acid Complex Crystal

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Protein-Nucleic Acid Complex Crystal

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Screen Structure

Protein-Nucleic Acid Complex Crystal

Well Count	48
Number of distinct ingredients	9
Internal diversity (score mean dist)	0.753

Screen Composition

More information about screen composition chart.

ingredient type	Protein-Nucleic Acid Complex Crystal
Buffer	48
Polymer	48
Salt	78

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Protein-Nucleic Acid Complex Crystal Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Polyethylene glycol 8,000 6 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	120.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 780.0 μl of water (100 v/v)
A2	Polyethylene glycol 8,000 16 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 480.0 μl of water (100 v/v)
A3	Polyethylene glycol 4,000 11 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.05 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 25.0 μl of Magnesium chloride (2 M) 220.0 μl of Polyethylene glycol 4,000 (50 w/v) 655.0 μl of water (100 v/v)
A4	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 450.0 μl of water (100 v/v)
A5	Polyethylene glycol 4,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.05 M - 🔍	pH 4.6	25.0 μl of Calcium chloride (2 M) 120.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 755.0 μl of water (100 v/v)
A6	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Magnesium chloride 0.05 M - Sodium chloride 0.1 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 25.0 μl of Magnesium chloride (2 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 505.0 μl of water (100 v/v)
A7	Polyethylene glycol 2,000 11 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 220.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 630.0 μl of water (100 v/v)
A8	Polyethylene glycol 2,000 20 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 425.0 μl of water (100 v/v)
B1	Polyethylene glycol 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Calcium chloride (2 M) 320.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 480.0 μl of water (100 v/v)
B2	Polyethylene glycol 2,000 25 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 300.0 μl of water (100 v/v)
B3	Polyethylene glycol 4,000 20 w/v - MES 0.1 M pH 6 Magnesium chloride 0.1 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 50.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 450.0 μl of water (100 v/v)
B4	Polyethylene glycol 8,000 11 w/v - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	220.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 680.0 μl of water (100 v/v)
B5	Polyethylene glycol 4,000 25 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 300.0 μl of water (100 v/v)
B6	Polyethylene glycol 2,000 20 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 450.0 μl of water (100 v/v)
B7	Polyethylene glycol 8,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Calcium chloride (2 M) 220.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 630.0 μl of water (100 v/v)
B8	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 120.0 μl of Polyethylene glycol 2,000 (50 w/v) 780.0 μl of water (100 v/v)
C1	Polyethylene glycol 2,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Magnesium chloride 0.05 M - 🔍	pH 4.6	25.0 μl of Magnesium chloride (2 M) 220.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 605.0 μl of water (100 v/v)
C2	Polyethylene glycol 4,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 450.0 μl of water (100 v/v)
C3	Polyethylene glycol 4,000 16 w/v - MES 0.1 M pH 6 Magnesium chloride 0.05 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 25.0 μl of Magnesium chloride (2 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 555.0 μl of water (100 v/v)
C4	Polyethylene glycol 8,000 6 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 120.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 680.0 μl of water (100 v/v)
C5	Polyethylene glycol 8,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Magnesium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 400.0 μl of water (100 v/v)
C6	Polyethylene glycol 4,000 11 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 220.0 μl of Polyethylene glycol 4,000 (50 w/v) 630.0 μl of water (100 v/v)
C7	Polyethylene glycol 2,000 16 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 2,000 (50 w/v) 580.0 μl of water (100 v/v)
C8	Polyethylene glycol 4,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	120.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 730.0 μl of water (100 v/v)
D1	Polyethylene glycol 2,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 4.6	25.0 μl of Calcium chloride (2 M) 320.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 505.0 μl of water (100 v/v)
D2	Polyethylene glycol 4,000 20 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 425.0 μl of water (100 v/v)
D3	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 6	50.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 120.0 μl of Polyethylene glycol 2,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 680.0 μl of water (100 v/v)
D4	Polyethylene glycol 8,000 11 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 220.0 μl of Polyethylene glycol 8,000 (50 w/v) 630.0 μl of water (100 v/v)
D5	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 475.0 μl of water (100 v/v)
D6	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 530.0 μl of water (100 v/v)
D7	Polyethylene glycol 8,000 11 w/v - MES 0.1 M pH 6 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 220.0 μl of Polyethylene glycol 8,000 (50 w/v) 680.0 μl of water (100 v/v)
D8	Polyethylene glycol 8,000 6 w/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 120.0 μl of Polyethylene glycol 8,000 (50 w/v) 780.0 μl of water (100 v/v)
E1	Polyethylene glycol 8,000 16 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 50.0 μl of Magnesium chloride (2 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 480.0 μl of water (100 v/v)
E2	Polyethylene glycol 2,000 20 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Calcium chloride (2 M) 400.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 400.0 μl of water (100 v/v)
E3	Polyethylene glycol 4,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - 🔍	pH 4.6	220.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 630.0 μl of water (100 v/v)
E4	Polyethylene glycol 4,000 6 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 120.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 705.0 μl of water (100 v/v)
E5	Polyethylene glycol 4,000 6 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 120.0 μl of Polyethylene glycol 4,000 (50 w/v) 755.0 μl of water (100 v/v)
E6	Polyethylene glycol 8,000 16 w/v - Sodium acetate 0.1 M pH 4.6 Calcium chloride 0.05 M - Sodium chloride 0.1 M - 🔍	pH 4.6	25.0 μl of Calcium chloride (2 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 505.0 μl of water (100 v/v)
E7	Polyethylene glycol 2,000 20 w/v - MES 0.1 M pH 6 Calcium chloride 0.1 M - 🔍	pH 6	50.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 2,000 (50 w/v) 450.0 μl of water (100 v/v)
E8	Polyethylene glycol 2,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Magnesium chloride (2 M) 220.0 μl of Polyethylene glycol 2,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 580.0 μl of water (100 v/v)
F1	Polyethylene glycol 4,000 11 w/v - Sodium acetate 0.1 M pH 4.6 Sodium chloride 0.1 M - Calcium chloride 0.1 M - 🔍	pH 4.6	50.0 μl of Calcium chloride (2 M) 220.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 50.0 μl of Sodium chloride (2 M) 580.0 μl of water (100 v/v)
F2	Polyethylene glycol 2,000 6 w/v - MES 0.1 M pH 6 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 120.0 μl of Polyethylene glycol 2,000 (50 w/v) 780.0 μl of water (100 v/v)
F3	Polyethylene glycol 8,000 20 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - Sodium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 400.0 μl of water (100 v/v)
F4	Polyethylene glycol 4,000 16 w/v - HEPES 0.1 M pH 7.5 Sodium chloride 0.1 M - Magnesium chloride 0.05 M - 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 25.0 μl of Magnesium chloride (2 M) 320.0 μl of Polyethylene glycol 4,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 505.0 μl of water (100 v/v)
F5	Polyethylene glycol 2,000 6 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.05 M - 🔍	pH 7.5	25.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 120.0 μl of Polyethylene glycol 2,000 (50 w/v) 755.0 μl of water (100 v/v)
F6	Polyethylene glycol 8,000 6 w/v - Sodium acetate 0.1 M pH 4.6 Magnesium chloride 0.05 M - 🔍	pH 4.6	25.0 μl of Magnesium chloride (2 M) 120.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 755.0 μl of water (100 v/v)
F7	Polyethylene glycol 8,000 11 w/v - MES 0.1 M pH 6 Sodium chloride 0.1 M - Calcium chloride 0.05 M - 🔍	pH 6	25.0 μl of Calcium chloride (2 M) 100.0 μl of MES pH 6 (1 M) 220.0 μl of Polyethylene glycol 8,000 (50 w/v) 50.0 μl of Sodium chloride (2 M) 605.0 μl of water (100 v/v)
F8	Polyethylene glycol 4,000 25 w/v - HEPES 0.1 M pH 7.5 Calcium chloride 0.1 M - 🔍	pH 7.5	50.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of Polyethylene glycol 4,000 (50 w/v) 350.0 μl of water (100 v/v)