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Supplemental Information

Screen Description

Berkeley

Rigaku / Berkeley
Created 2018-03-08.

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Berkeley

    Screen Structure

    Berkeley

    Well Count 96
    Number of distinct ingredients 41
    Internal diversity (score mean dist) 0.927

    Screen Composition

    2025-06-14T13:44:17.223898 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Berkeley
    Buffer 112
    Organic (non-volatile) 27
    Organic (volatile) 7
    Polymer 74
    Salt 109

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Berkeley Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium chloride 2.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Calcium chloride 0.05 M -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    50 μl of Calcium chloride (1 M)
    440 μl of Sodium chloride (5 M)
    410 μl of water (100 v/v)
    A2
    • Sodium formate 2 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    50 μl of Sodium acetate pH 4.5 (2 M)
    400 μl of Sodium formate (5 M)
    550 μl of water (100 v/v)
    A3
    • Ammonium sulfate 2 M -
    • tri-Sodium citrate 0.1 M pH 5.3
    pH 5.3
    100 μl of tri-Sodium citrate pH 5.3 (1 M)
    500 μl of Ammonium sulfate (4 M)
    400 μl of water (100 v/v)
    A4
    • 2-Propanol 10 v/v -
    • Ammonium sulfate 1.8 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    450 μl of Ammonium sulfate (4 M)
    350 μl of water (100 v/v)
    A5
    • di-Sodium malonate 2.2 M pH 5
    pH 5
    647 μl of di-Sodium malonate pH 5 (3.4 M)
    352 μl of water (100 v/v)
    A6
    • di-Sodium malonate 2.2 M pH 7
    pH 7
    647 μl of di-Sodium malonate pH 7 (3.4 M)
    352 μl of water (100 v/v)
    A7
    • di-Sodium malonate 2.2 M -
    NA
    647 μl of di-Sodium malonate pH 8 (3.4 M)
    352 μl of water (100 v/v)
    A8
    • Pentaerythritol Propoxylate (5/4 PO/OH) 30 v/v -
    • HEPES 0.1 M pH 7.5
    • Potassium chloride 0.2 M -
    pH 7.5
    600 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of Potassium chloride (4 M)
    250 μl of water (100 v/v)
    A9
    • Pentaerythritol Propoxylate (5/4 PO/OH) 30 v/v -
    • Sodium acetate 0.1 M pH 4.5
    • Magnesium chloride 0.1 M -
    pH 4.5
    600 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    50 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    A10
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium acetate 0.2 M -
    pH 6.5
    500 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of water (100 v/v)
    A11
    • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
    • MES 0.1 M pH 5.5
    • Sodium chloride 0.4 M -
    pH 5.5
    500 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    400 μl of Sodium chloride (1 M)
    100 μl of MES pH 5.5 (1 M)
    A12
    • Polyethylene glycol 3,350 35 w/v -
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    700 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of water (100 v/v)
    B1
    • Polyethylene glycol 3,350 30 w/v -
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Sodium chloride (5 M)
    220 μl of water (100 v/v)
    B2
    • Polyethylene glycol 3,350 30 w/v -
    • Sodium chloride 0.4 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Sodium chloride (5 M)
    220 μl of water (100 v/v)
    B3
    • Polyethylene glycol 3,350 30 w/v -
    • Sodium chloride 0.4 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Sodium chloride (5 M)
    220 μl of water (100 v/v)
    B4
    • Polyethylene glycol 3,350 25 w/v -
    • Potassium Sodium tartrate 0.1 M -
    • Magnesium chloride 0.005 M -
    NA
    66 μl of Potassium Sodium tartrate (1.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    2 μl of Magnesium chloride pH 7 (2.5 M)
    431 μl of water (100 v/v)
    B5
    • Polyethylene glycol 3,350 20 w/v -
    • Lithium sulfate 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    520 μl of water (100 v/v)
    B6
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium fluoride 0.2 M -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    250 μl of Sodium fluoride (0.8 M)
    350 μl of water (100 v/v)
    B7
    • Polyethylene glycol 3,350 25 w/v -
    • BIS-TRIS propane 0.1 M pH 6.4
    pH 6.4
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    17 μl of BIS-TRIS propane pH 5.5 (1 M)
    82 μl of BIS-TRIS propane pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B8
    • Polyethylene glycol 3,350 25 w/v -
    • BIS-TRIS propane 0.1 M pH 7.6
    pH 7.6
    100 μl of BIS-TRIS propane pH 7.6 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    B9
    • Polyethylene glycol 3,350 25 w/v -
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    B10
    • Polyethylene glycol 3,350 25 w/v -
    • Magnesium chloride 0.025 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    12 μl of Magnesium chloride (2 M)
    387 μl of water (100 v/v)
    B11
    • Polyethylene glycol 3,350 25 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 0.2 M -
    pH 8.5
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    333 μl of water (100 v/v)
    B12
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    333 μl of water (100 v/v)
    C1
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    333 μl of water (100 v/v)
    C2
    • Polyethylene glycol 3,350 25 w/v -
    • MES 0.1 M pH 5.5
    • Ammonium sulfate 0.2 M -
    pH 5.5
    66 μl of Ammonium sulfate (3 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of MES pH 5.5 (1 M)
    333 μl of water (100 v/v)
    C3
    • Polyethylene glycol 3,350 20 w/v -
    • Calcium chloride 0.1 M -
    NA
    100 μl of Calcium chloride (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    C4
    • Polyethylene glycol 3,350 20 w/v -
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Magnesium chloride (2 M)
    100 μl of MES pH 5.5 (1 M)
    450 μl of water (100 v/v)
    C5
    • Polyethylene glycol 3,350 15 w/v -
    • tri-Sodium citrate 0.3 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    187 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    412 μl of water (100 v/v)
    C6
    • Polyethylene glycol 3,350 25 w/v -
    • Potassium chloride 0.2 M -
    NA
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Potassium chloride (4 M)
    450 μl of water (100 v/v)
    C7
    • Polyethylene glycol 3,350 25 w/v -
    • di-Ammonium hydrogen phosphate 0.1 M -
    NA
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    471 μl of water (100 v/v)
    C8
    • Polyethylene glycol 3,350 20 w/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    NA
    100 μl of Sodium chloride (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Magnesium chloride (2 M)
    450 μl of water (100 v/v)
    C9
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    33 μl of Sodium acetate (3 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    366 μl of water (100 v/v)
    C10
    • Polyethylene glycol 3,350 20 w/v -
    • 2-Propanol 5 v/v -
    • di-Ammonium hydrogen phosphate 0.1 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of 2-Propanol (100 v/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    471 μl of water (100 v/v)
    C11
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.1 M -
    • CBTP buffer 0.1 M pH 6.4
    pH 6.4
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of CBTP buffer pH 6.4 (1 M)
    100 μl of di-Sodium malonate pH 7 (1 M)
    400 μl of water (100 v/v)
    C12
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.1 M -
    • BIS-TRIS propane 0.1 M pH 7.6
    pH 7.6
    100 μl of BIS-TRIS propane pH 7.6 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Sodium malonate pH 7 (1 M)
    400 μl of water (100 v/v)
    D1
    • Polyethylene glycol 3,350 20 w/v -
    • di-Sodium malonate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of di-Sodium malonate pH 7 (1 M)
    400 μl of water (100 v/v)
    D2
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    133 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    366 μl of water (100 v/v)
    D3
    • Polyethylene glycol 3,350 20 w/v -
    • Potassium Sodium tartrate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    133 μl of Potassium Sodium tartrate (1.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    366 μl of water (100 v/v)
    D4
    • Polyethylene glycol 3,350 25 w/v -
    • Sodium acetate 0.2 M pH 4.5
    pH 4.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (2 M)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    D6
    • Polyethylene glycol 3,350 25 w/v -
    • Ammonium acetate 0.2 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of MES pH 5.5 (1 M)
    200 μl of water (100 v/v)
    D7
    • Polyethylene glycol 3,350 30 w/v -
    • Potassium chloride 0.1 M -
    • di-Potassium hydrogen phosphate 0.1 M -
    NA
    600 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium chloride (4 M)
    33 μl of di-Potassium hydrogen phosphate (3 M)
    341 μl of water (100 v/v)
    D8
    • Polyethylene glycol 3,350 20 w/v -
    • 2-Propanol 5 v/v -
    • di-Ammonium hydrogen citrate 0.1 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    40 μl of di-Ammonium hydrogen citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of 2-Propanol (100 v/v)
    100 μl of MES pH 5.5 (1 M)
    410 μl of water (100 v/v)
    D9
    • Polyethylene glycol 3,350 20 w/v -
    • 2-Propanol 5 v/v -
    • di-Ammonium hydrogen phosphate 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    28 μl of di-Ammonium hydrogen phosphate (3.5 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of 2-Propanol (100 v/v)
    421 μl of water (100 v/v)
    D10
    • Polyethylene glycol 3,350 20 w/v -
    • 1,6-Hexanediol 10 w/v -
    • Magnesium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    333 μl of 1,6-Hexanediol (30 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Lithium sulfate (2.5 M)
    50 μl of Magnesium chloride (2 M)
    76 μl of water (100 v/v)
    D11
    • Polyethylene glycol 3,350 20 w/v -
    • 1,6-Hexanediol 10 w/v -
    • Sodium chloride 0.1 M -
    • Lithium sulfate 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    333 μl of 1,6-Hexanediol (30 w/v)
    100 μl of Sodium chloride (1 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Lithium sulfate (2.5 M)
    26 μl of water (100 v/v)
    D12
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    550 μl of water (100 v/v)
    E1
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    • Sodium chloride 0.2 M -
    NA
    200 μl of Sodium chloride (1 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of water (100 v/v)
    E2
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    • Ammonium sulfate 0.2 M -
    NA
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    66 μl of Ammonium sulfate (3 M)
    433 μl of water (100 v/v)
    E3
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    • Magnesium chloride 0.6 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of Magnesium chloride (2 M)
    100 μl of MES pH 5.5 (1 M)
    300 μl of water (100 v/v)
    E4
    • Ammonium sulfate 1.5 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    471 μl of water (100 v/v)
    E5
    • Ammonium sulfate 1.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Ammonium sulfate (3 M)
    500 μl of water (100 v/v)
    E6
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • di-Sodium malonate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    pH 5.5
    58 μl of di-Sodium malonate pH 5 (3.4 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    441 μl of water (100 v/v)
    E7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • di-Sodium malonate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    E8
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • di-Sodium malonate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    441 μl of water (100 v/v)
    E9
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • di-Sodium malonate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    58 μl of di-Sodium malonate pH 8 (3.4 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    441 μl of water (100 v/v)
    E10
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    • 1,6-Hexanediol 10 w/v -
    • Sodium chloride 0.2 M -
    NA
    333 μl of 1,6-Hexanediol (30 w/v)
    200 μl of Sodium chloride (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    66 μl of water (100 v/v)
    E11
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    • di-Ammonium hydrogen phosphate 0.2 M -
    NA
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    692 μl of water (100 v/v)
    E12
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    • di-Sodium tartrate 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    133 μl of di-Sodium tartrate (1.5 M)
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    516 μl of water (100 v/v)
    F1
    • Polyethylene glycol monomethyl ether 550 10 v/v -
    • Ammonium sulfate 0.8 M -
    NA
    266 μl of Ammonium sulfate (3 M)
    100 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    633 μl of water (100 v/v)
    F2
    • Polyethylene glycol monomethyl ether 550 25 v/v -
    • Magnesium chloride 0.2 M -
    NA
    250 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Magnesium chloride (2 M)
    650 μl of water (100 v/v)
    F3
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    • Ammonium acetate 0.2 M -
    NA
    200 μl of Ammonium acetate (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    400 μl of water (100 v/v)
    F4
    • Polyethylene glycol monomethyl ether 5,000 5 w/v -
    • Sodium formate 2 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    285 μl of Sodium formate (7 M)
    100 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of MES pH 5.5 (1 M)
    514 μl of water (100 v/v)
    F5
    • Polyethylene glycol monomethyl ether 5,000 5 w/v -
    • Sodium formate 2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    285 μl of Sodium formate (7 M)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    514 μl of water (100 v/v)
    F6
    • Polyethylene glycol monomethyl ether 5,000 5 w/v -
    • Sodium formate 2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    285 μl of Sodium formate (7 M)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    514 μl of water (100 v/v)
    F7
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    80 μl of Lithium sulfate (2.5 M)
    420 μl of water (100 v/v)
    F8
    • 2-methyl-2,4-pentanediol BCC 55 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    550 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    350 μl of water (100 v/v)
    F9
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • MES 0.1 M pH 6.5
    pH 6.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    F10
    • 2-methyl-2,4-pentanediol BCC 12 v/v -
    • Magnesium chloride 0.01 M -
    • tri-Sodium citrate 0.05 M -
    • HEPES 0.05 M pH 7.2
    pH 7.2
    120 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of tri-Sodium citrate pH 5.8 (1 M)
    18 μl of HEPES pH 7.5 (1 M)
    32 μl of HEPES pH 7 (1 M)
    5 μl of Magnesium chloride (2 M)
    775 μl of water (100 v/v)
    F11
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • MES 0.1 M pH 5.5
    • Sodium acetate 0.1 M -
    pH 5.5
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    33 μl of Sodium acetate (3 M)
    100 μl of MES pH 5.5 (1 M)
    466 μl of water (100 v/v)
    F12
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Sodium formate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    pH 5.5
    14 μl of Sodium formate (7 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    535 μl of water (100 v/v)
    G1
    • 2-methyl-2,4-pentanediol BCC 25 v/v -
    • Lithium sulfate 0.4 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    250 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    160 μl of Lithium sulfate (2.5 M)
    490 μl of water (100 v/v)
    G2
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • di-Sodium malonate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    391 μl of water (100 v/v)
    G3
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • tri-Sodium citrate 0.1 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    62 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    537 μl of water (100 v/v)
    G4
    • 2-methyl-2,4-pentanediol BCC 20 v/v -
    • Magnesium chloride 0.4 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    200 μl of Magnesium chloride (2 M)
    550 μl of water (100 v/v)
    G5
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • Potassium chloride 0.1 M -
    • CBTP buffer 0.1 M pH 6.4
    pH 6.4
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of CBTP buffer pH 6.4 (1 M)
    25 μl of Potassium chloride (4 M)
    725 μl of water (100 v/v)
    G6
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    • di-Ammonium tartrate 0.5 M -
    NA
    100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of di-Ammonium tartrate (2 M)
    650 μl of water (100 v/v)
    G7
    • 2-methyl-2,4-pentanediol BCC 5 v/v -
    • Ammonium sulfate 1.5 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    50 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of Ammonium sulfate (3 M)
    50 μl of Sodium acetate pH 4.5 (2 M)
    400 μl of water (100 v/v)
    G8
    • 2-methyl-2,4-pentanediol BCC 15 v/v -
    • Ammonium di-hydrogen phosphate 0.8 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    150 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    320 μl of Ammonium di-hydrogen phosphate (2.5 M)
    430 μl of water (100 v/v)
    G9
    • Polyethylene glycol 400 45 v/v -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    G10
    • Polyethylene glycol 400 30 v/v -
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium chloride (2 M)
    500 μl of water (100 v/v)
    G11
    • Polyethylene glycol 400 25 v/v -
    • Magnesium chloride 0.6 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    300 μl of Magnesium chloride (2 M)
    350 μl of water (100 v/v)
    G12
    • Polyethylene glycol 400 25 v/v -
    • Cadmium chloride 0.02 M -
    • BIS-TRIS propane 0.1 M pH 8.2
    pH 8.2
    100 μl of BIS-TRIS propane pH 8.2 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Cadmium chloride (1 M)
    630 μl of water (100 v/v)
    H1
    • Polyethylene glycol 400 25 v/v -
    • di-Sodium tartrate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    pH 4.5
    133 μl of di-Sodium tartrate (1.5 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium acetate pH 4.5 (2 M)
    566 μl of water (100 v/v)
    H2
    • Polyethylene glycol 400 20 v/v -
    • MES 0.1 M pH 5.5
    • Sodium fluoride 0.2 M -
    pH 5.5
    200 μl of Polyethylene glycol 400 (100 v/v)
    250 μl of Sodium fluoride (0.8 M)
    100 μl of MES pH 5.5 (1 M)
    450 μl of water (100 v/v)
    H3
    • Polyethylene glycol 400 25 v/v -
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.2 M -
    NA
    100 μl of Sodium chloride (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium chloride (2 M)
    550 μl of water (100 v/v)
    H4
    • Polyethylene glycol 400 25 v/v -
    • Sodium acetate 0.1 M -
    • CBTP buffer 0.1 M pH 6.4
    pH 6.4
    33 μl of Sodium acetate (3 M)
    100 μl of CBTP buffer pH 6.4 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    616 μl of water (100 v/v)
    H5
    • Polyethylene glycol 400 25 v/v -
    • Lithium sulfate 0.1 M -
    • Magnesium chloride 0.1 M -
    NA
    250 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Lithium sulfate (2.5 M)
    50 μl of Magnesium chloride (2 M)
    660 μl of water (100 v/v)
    H6
    • Polyethylene glycol 1,500 20 w/v -
    • 1,6-Hexanediol 12 w/v -
    • Calcium chloride 0.1 M -
    • MES 0.1 M pH 5.5
    pH 5.5
    400 μl of 1,6-Hexanediol (30 w/v)
    100 μl of Calcium chloride (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of MES pH 5.5 (1 M)
    H7
    • Polyethylene glycol 1,500 20 w/v -
    • 2-Propanol 5 v/v -
    • Cadmium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    50 μl of 2-Propanol (100 v/v)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Cadmium chloride (1 M)
    350 μl of water (100 v/v)
    H8
    • Polyethylene glycol 4,000 30 w/v -
    • Ammonium acetate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    pH 4.6
    200 μl of Ammonium acetate (1 M)
    600 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of water (100 v/v)
    H9
    • Polyethylene glycol 4,000 25 w/v -
    • 2-Propanol 10 v/v -
    • HEPES 0.1 M pH 7.5
    pH 7.5
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    H10
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    pH 6.5
    66 μl of Sodium acetate (3 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of BIS-TRIS pH 6.5 (1 M)
    333 μl of water (100 v/v)
    H11
    • Polyethylene glycol 8,000 20 w/v -
    • 1,6-Hexanediol 10 w/v -
    • Calcium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    NA
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    333 μl of 1,6-Hexanediol (30 w/v)
    100 μl of Calcium chloride (1 M)
    50 μl of Magnesium chloride (2 M)
    116 μl of water (100 v/v)
    H12
    • Polyethylene glycol 10,000 15 w/v -
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Ammonium acetate (1 M)
    300 μl of Polyethylene glycol 10,000 (50 w/v)
    400 μl of water (100 v/v)