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Supplemental Information

Screen Description

Berkeley

Rigaku / Berkeley
Created 2018-03-08.

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Barcodes

Berkeley

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Screen Structure

Berkeley

Well Count 96
Number of distinct ingredients 41
Internal diversity (score mean dist) 0.927

Screen Composition

2025-08-16T06:24:32.856095 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Berkeley
Buffer 112
Organic (non-volatile) 27
Organic (volatile) 7
Polymer 74
Salt 109

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Berkeley Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium chloride 2.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Calcium chloride 0.05 M -
pH 8
50.0 μl of Calcium chloride (1 M)
440.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
410.0 μl of water (100 v/v)
A2
  • Sodium formate 2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
50.0 μl of Sodium acetate pH 4.5 (2 M)
400.0 μl of Sodium formate (5 M)
550.0 μl of water (100 v/v)
A3
  • Ammonium sulfate 2 M -
  • tri-Sodium citrate 0.1 M pH 5.3
pH 5.3
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of tri-Sodium citrate pH 5.3 (1 M)
400.0 μl of water (100 v/v)
A4
  • 2-Propanol 10 v/v -
  • Ammonium sulfate 1.8 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of 2-Propanol (100 v/v)
450.0 μl of Ammonium sulfate (4 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
350.0 μl of water (100 v/v)
A5
  • di-Sodium malonate 2.2 M pH 5
pH 5
647.1 μl of di-Sodium malonate pH 5 (3.4 M)
352.9 μl of water (100 v/v)
A6
  • di-Sodium malonate 2.2 M pH 7
pH 7
647.1 μl of di-Sodium malonate pH 7 (3.4 M)
352.9 μl of water (100 v/v)
A7
  • di-Sodium malonate 2.2 M -
NA
647.1 μl of di-Sodium malonate pH 8 (3.4 M)
352.9 μl of water (100 v/v)
A8
  • Pentaerythritol Propoxylate (5/4 PO/OH) 30 v/v -
  • HEPES 0.1 M pH 7.5
  • Potassium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
50.0 μl of Potassium chloride (4 M)
250.0 μl of water (100 v/v)
A9
  • Pentaerythritol Propoxylate (5/4 PO/OH) 30 v/v -
  • Sodium acetate 0.1 M pH 4.5
  • Magnesium chloride 0.1 M -
pH 4.5
50.0 μl of Magnesium chloride (2 M)
600.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
300.0 μl of water (100 v/v)
A10
  • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium acetate 0.2 M -
pH 6.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
200.0 μl of water (100 v/v)
A11
  • Pentaerythritol Propoxylate (5/4 PO/OH) 25 v/v -
  • MES 0.1 M pH 5.5
  • Sodium chloride 0.4 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
500.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
400.0 μl of Sodium chloride (1 M)
A12
  • Polyethylene glycol 3,350 35 w/v -
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 3,350 30 w/v -
  • Sodium chloride 0.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
220.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 3,350 30 w/v -
  • Sodium chloride 0.4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Sodium chloride (5 M)
220.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 30 w/v -
  • Sodium chloride 0.4 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of Sodium chloride (5 M)
220.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 3,350 25 w/v -
  • Potassium Sodium tartrate 0.1 M -
  • Magnesium chloride 0.005 M -
NA
2.0 μl of Magnesium chloride pH 7 (2.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
66.7 μl of Potassium Sodium tartrate (1.5 M)
431.3 μl of water (100 v/v)
B5
  • Polyethylene glycol 3,350 20 w/v -
  • Lithium sulfate 0.2 M -
NA
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of Sodium fluoride (0.8 M)
350.0 μl of water (100 v/v)
B7
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS propane 0.1 M pH 6.4
pH 6.4
17.2 μl of BIS-TRIS propane pH 5.5 (1 M)
82.8 μl of BIS-TRIS propane pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B8
  • Polyethylene glycol 3,350 25 w/v -
  • BIS-TRIS propane 0.1 M pH 7.6
pH 7.6
100.0 μl of BIS-TRIS propane pH 7.6 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 3,350 25 w/v -
  • Magnesium chloride 0.025 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
12.5 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
387.5 μl of water (100 v/v)
B11
  • Polyethylene glycol 3,350 25 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 0.2 M -
pH 8.5
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
333.3 μl of water (100 v/v)
B12
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
C1
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
C2
  • Polyethylene glycol 3,350 25 w/v -
  • MES 0.1 M pH 5.5
  • Ammonium sulfate 0.2 M -
pH 5.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
333.3 μl of water (100 v/v)
C3
  • Polyethylene glycol 3,350 20 w/v -
  • Calcium chloride 0.1 M -
NA
100.0 μl of Calcium chloride (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
C4
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
450.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 3,350 15 w/v -
  • tri-Sodium citrate 0.3 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
187.5 μl of tri-Sodium citrate (1.6 M)
412.5 μl of water (100 v/v)
C6
  • Polyethylene glycol 3,350 25 w/v -
  • Potassium chloride 0.2 M -
NA
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium chloride (4 M)
450.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 3,350 25 w/v -
  • di-Ammonium hydrogen phosphate 0.1 M -
NA
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
471.4 μl of water (100 v/v)
C8
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
NA
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium chloride (1 M)
450.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
33.3 μl of Sodium acetate (3 M)
366.7 μl of water (100 v/v)
C10
  • Polyethylene glycol 3,350 20 w/v -
  • 2-Propanol 5 v/v -
  • di-Ammonium hydrogen phosphate 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
50.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
471.4 μl of water (100 v/v)
C11
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.1 M -
  • CBTP buffer 0.1 M pH 6.4
pH 6.4
100.0 μl of CBTP buffer pH 6.4 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium malonate pH 7 (1 M)
400.0 μl of water (100 v/v)
C12
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.1 M -
  • BIS-TRIS propane 0.1 M pH 7.6
pH 7.6
100.0 μl of BIS-TRIS propane pH 7.6 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium malonate pH 7 (1 M)
400.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium malonate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium malonate pH 7 (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D2
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
366.7 μl of water (100 v/v)
D3
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium Sodium tartrate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
366.7 μl of water (100 v/v)
D4
  • Polyethylene glycol 3,350 25 w/v -
  • Sodium acetate 0.2 M pH 4.5
pH 4.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (2 M)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 3,350 25 w/v -
  • Ammonium acetate 0.2 M -
  • MES 0.1 M pH 5.5
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of MES pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 3,350 30 w/v -
  • Potassium chloride 0.1 M -
  • di-Potassium hydrogen phosphate 0.1 M -
NA
600.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium chloride (4 M)
33.3 μl of di-Potassium hydrogen phosphate (3 M)
341.7 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 20 w/v -
  • 2-Propanol 5 v/v -
  • di-Ammonium hydrogen citrate 0.1 M -
  • MES 0.1 M pH 5.5
pH 5.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of MES pH 5.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of di-Ammonium hydrogen citrate (2.5 M)
410.0 μl of water (100 v/v)
D9
  • Polyethylene glycol 3,350 20 w/v -
  • 2-Propanol 5 v/v -
  • di-Ammonium hydrogen phosphate 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
421.4 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 20 w/v -
  • 1,6-Hexanediol 10 w/v -
  • Magnesium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
333.3 μl of 1,6-Hexanediol (30 w/v)
100.0 μl of HEPES pH 7.5 (1 M)
40.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
76.7 μl of water (100 v/v)
D11
  • Polyethylene glycol 3,350 20 w/v -
  • 1,6-Hexanediol 10 w/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
333.3 μl of 1,6-Hexanediol (30 w/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
40.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium chloride (1 M)
26.7 μl of water (100 v/v)
D12
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
550.0 μl of water (100 v/v)
E1
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
  • Sodium chloride 0.2 M -
NA
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
E2
  • Polyethylene glycol monomethyl ether 2,000 25 w/v -
  • Ammonium sulfate 0.2 M -
NA
66.7 μl of Ammonium sulfate (3 M)
500.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
433.3 μl of water (100 v/v)
E3
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
  • Magnesium chloride 0.6 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
300.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
300.0 μl of water (100 v/v)
E4
  • Ammonium sulfate 1.5 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
471.4 μl of water (100 v/v)
E5
  • Ammonium sulfate 1.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
400.0 μl of Ammonium sulfate (3 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of water (100 v/v)
E6
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • di-Sodium malonate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate pH 5 (3.4 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
441.2 μl of water (100 v/v)
E7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • di-Sodium malonate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
441.2 μl of water (100 v/v)
E8
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • di-Sodium malonate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
441.2 μl of water (100 v/v)
E9
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • di-Sodium malonate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
58.8 μl of di-Sodium malonate pH 8 (3.4 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
441.2 μl of water (100 v/v)
E10
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
  • 1,6-Hexanediol 10 w/v -
  • Sodium chloride 0.2 M -
NA
333.3 μl of 1,6-Hexanediol (30 w/v)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
66.7 μl of water (100 v/v)
E11
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • di-Ammonium hydrogen phosphate 0.2 M -
NA
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
692.9 μl of water (100 v/v)
E12
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • di-Sodium tartrate 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
133.3 μl of di-Sodium tartrate (1.5 M)
516.7 μl of water (100 v/v)
F1
  • Polyethylene glycol monomethyl ether 550 10 v/v -
  • Ammonium sulfate 0.8 M -
NA
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
633.3 μl of water (100 v/v)
F2
  • Polyethylene glycol monomethyl ether 550 25 v/v -
  • Magnesium chloride 0.2 M -
NA
100.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
650.0 μl of water (100 v/v)
F3
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • Ammonium acetate 0.2 M -
NA
200.0 μl of Ammonium acetate (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
400.0 μl of water (100 v/v)
F4
  • Polyethylene glycol monomethyl ether 5,000 5 w/v -
  • Sodium formate 2 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
100.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
285.7 μl of Sodium formate (7 M)
514.3 μl of water (100 v/v)
F5
  • Polyethylene glycol monomethyl ether 5,000 5 w/v -
  • Sodium formate 2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
285.7 μl of Sodium formate (7 M)
514.3 μl of water (100 v/v)
F6
  • Polyethylene glycol monomethyl ether 5,000 5 w/v -
  • Sodium formate 2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
285.7 μl of Sodium formate (7 M)
514.3 μl of water (100 v/v)
F7
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Lithium sulfate (2.5 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
F8
  • 2-methyl-2,4-pentanediol BCC 55 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
550.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
350.0 μl of water (100 v/v)
F9
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • MES 0.1 M pH 6.5
pH 6.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 6.5 (1 M)
600.0 μl of water (100 v/v)
F10
  • 2-methyl-2,4-pentanediol BCC 12 v/v -
  • Magnesium chloride 0.01 M -
  • tri-Sodium citrate 0.05 M -
  • HEPES 0.05 M pH 7.2
pH 7.2
120.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
32.0 μl of HEPES pH 7 (1 M)
18.0 μl of HEPES pH 7.5 (1 M)
5.0 μl of Magnesium chloride (2 M)
50.0 μl of tri-Sodium citrate pH 5.8 (1 M)
775.0 μl of water (100 v/v)
F11
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • MES 0.1 M pH 5.5
  • Sodium acetate 0.1 M -
pH 5.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of MES pH 5.5 (1 M)
33.3 μl of Sodium acetate (3 M)
466.7 μl of water (100 v/v)
F12
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Sodium formate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
14.3 μl of Sodium formate (7 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
535.7 μl of water (100 v/v)
G1
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
  • Lithium sulfate 0.4 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
160.0 μl of Lithium sulfate (2.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
490.0 μl of water (100 v/v)
G2
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • di-Sodium malonate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
391.2 μl of water (100 v/v)
G3
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • tri-Sodium citrate 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
62.5 μl of tri-Sodium citrate (1.6 M)
537.5 μl of water (100 v/v)
G4
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
  • Magnesium chloride 0.4 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Magnesium chloride (2 M)
50.0 μl of Sodium acetate pH 4.5 (2 M)
550.0 μl of water (100 v/v)
G5
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • Potassium chloride 0.1 M -
  • CBTP buffer 0.1 M pH 6.4
pH 6.4
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of CBTP buffer pH 6.4 (1 M)
25.0 μl of Potassium chloride (4 M)
725.0 μl of water (100 v/v)
G6
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • di-Ammonium tartrate 0.5 M -
NA
100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
250.0 μl of di-Ammonium tartrate (2 M)
650.0 μl of water (100 v/v)
G7
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
  • Ammonium sulfate 1.5 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
50.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
500.0 μl of Ammonium sulfate (3 M)
50.0 μl of Sodium acetate pH 4.5 (2 M)
400.0 μl of water (100 v/v)
G8
  • 2-methyl-2,4-pentanediol BCC 15 v/v -
  • Ammonium di-hydrogen phosphate 0.8 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
150.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
320.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
430.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 400 45 v/v -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 400 30 v/v -
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
500.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 400 25 v/v -
  • Magnesium chloride 0.6 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
300.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
350.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 400 25 v/v -
  • Cadmium chloride 0.02 M -
  • BIS-TRIS propane 0.1 M pH 8.2
pH 8.2
100.0 μl of BIS-TRIS propane pH 8.2 (1 M)
20.0 μl of Cadmium chloride (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
630.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 400 25 v/v -
  • di-Sodium tartrate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
250.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium acetate pH 4.5 (2 M)
133.3 μl of di-Sodium tartrate (1.5 M)
566.7 μl of water (100 v/v)
H2
  • Polyethylene glycol 400 20 v/v -
  • MES 0.1 M pH 5.5
  • Sodium fluoride 0.2 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
250.0 μl of Sodium fluoride (0.8 M)
450.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 400 25 v/v -
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.2 M -
NA
100.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
550.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 400 25 v/v -
  • Sodium acetate 0.1 M -
  • CBTP buffer 0.1 M pH 6.4
pH 6.4
100.0 μl of CBTP buffer pH 6.4 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium acetate (3 M)
616.7 μl of water (100 v/v)
H5
  • Polyethylene glycol 400 25 v/v -
  • Lithium sulfate 0.1 M -
  • Magnesium chloride 0.1 M -
NA
40.0 μl of Lithium sulfate (2.5 M)
50.0 μl of Magnesium chloride (2 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
660.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 1,500 20 w/v -
  • 1,6-Hexanediol 12 w/v -
  • Calcium chloride 0.1 M -
  • MES 0.1 M pH 5.5
pH 5.5
400.0 μl of 1,6-Hexanediol (30 w/v)
100.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 5.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
H7
  • Polyethylene glycol 1,500 20 w/v -
  • 2-Propanol 5 v/v -
  • Cadmium chloride 0.1 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
50.0 μl of 2-Propanol (100 v/v)
100.0 μl of Cadmium chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
350.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
200.0 μl of Ammonium acetate (1 M)
600.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 4,000 25 w/v -
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 4,000 (50 w/v)
66.7 μl of Sodium acetate (3 M)
333.3 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 20 w/v -
  • 1,6-Hexanediol 10 w/v -
  • Calcium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
NA
333.3 μl of 1,6-Hexanediol (30 w/v)
100.0 μl of Calcium chloride (1 M)
50.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
116.7 μl of water (100 v/v)
H12
  • Polyethylene glycol 10,000 15 w/v -
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
pH 8
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 10,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
400.0 μl of water (100 v/v)