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Supplemental Information

Screen Description

Index Screen

Hampton Research / Index Screen
Created 2018-06-12.

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Index Screen

    Screen Structure

    Index Screen

    Well Count 96
    Number of distinct ingredients 48
    Internal diversity (score mean dist) 0.907

    Screen Composition

    2025-06-13T21:33:16.491870 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Index Screen
    Buffer 113
    Complex ingredient 4
    Organic (non-volatile) 8
    Polymer 59
    Salt 95

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Index Screen Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.1 M pH 3.5
    • Ammonium sulfate 2 M -
    pH 3.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    328 μl of water (100 v/v)
    A2
    • Sodium acetate 0.1 M pH 4.5
    • Ammonium sulfate 2 M -
    pH 4.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    328 μl of water (100 v/v)
    A3
    • BIS-TRIS 0.1 M pH 5.5
    • Ammonium sulfate 2 M -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    A4
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 7.5
    • Ammonium sulfate 2 M -
    pH 7.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    328 μl of water (100 v/v)
    A6
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    pH 8.5
    571 μl of Ammonium sulfate (3.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    328 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.1 M pH 3.5
    • Sodium chloride 3 M -
    pH 3.5
    600 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    300 μl of water (100 v/v)
    A8
    • Sodium acetate 0.1 M pH 4.5
    • Sodium chloride 3 M -
    pH 4.5
    600 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    300 μl of water (100 v/v)
    A9
    • BIS-TRIS 0.1 M pH 5.5
    • Sodium chloride 3 M -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    A10
    • BIS-TRIS 0.1 M pH 6.5
    • Sodium chloride 3 M -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    A11
    • HEPES 0.1 M pH 7.5
    • Sodium chloride 3 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)
    A12
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium chloride 3 M -
    pH 8.5
    600 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    B1
    • BIS-TRIS 0.1 M pH 5.5
    • Magnesium formate 0.3 M -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    300 μl of Magnesium formate (1 M)
    600 μl of water (100 v/v)
    B2
    • BIS-TRIS 0.1 M pH 6.5
    • Magnesium formate 0.5 M -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    B3
    • HEPES 0.1 M pH 7.5
    • Magnesium formate 0.5 M -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    B4
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium formate 0.3 M -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Magnesium formate (1 M)
    600 μl of water (100 v/v)
    B5
    • Sodium di-hydrogen phosphate 1.26 M -
    • di-Potassium hydrogen phosphate 0.14 M -
    NA
    315 μl of Sodium di-hydrogen phosphate (4 M)
    35 μl of di-Potassium hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    B6
    • Sodium di-hydrogen phosphate 0.49 M -
    • di-Potassium hydrogen phosphate 0.91 M -
    NA
    122 μl of Sodium di-hydrogen phosphate (4 M)
    227 μl of di-Potassium hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    B7
    • Sodium di-hydrogen phosphate 0.056 M -
    • di-Potassium hydrogen phosphate 1.34 M -
    NA
    14 μl of Sodium di-hydrogen phosphate (4 M)
    336 μl of di-Potassium hydrogen phosphate (4 M)
    650 μl of water (100 v/v)
    B8
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    pH 7.5
    875 μl of tri-Sodium citrate (1.6 M)
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of water (100 v/v)
    B9
    • tri-Ammonium citrate 1.8 M -
    NA
    720 μl of tri-Ammonium citrate (2.5 M)
    280 μl of water (100 v/v)
    B10
    • di-Sodium succinate 0.8 M -
    NA
    666 μl of di-Sodium succinate (1.2 M)
    333 μl of water (100 v/v)
    B11
    • di-Sodium DL-malate 2.1 M -
    NA
    700 μl of di-Sodium DL-malate (3 M)
    300 μl of water (100 v/v)
    B12
    • Sodium acetate 2.8 M -
    NA
    700 μl of Sodium acetate (4 M)
    300 μl of water (100 v/v)
    C1
    • Sodium formate 3.5 M -
    NA
    700 μl of Sodium formate (5 M)
    300 μl of water (100 v/v)
    C2
    • di-Ammonium tartrate 1.1 M -
    NA
    687 μl of di-Ammonium tartrate (1.6 M)
    312 μl of water (100 v/v)
    C3
    • di-Sodium malonate 2.4 M -
    NA
    705 μl of di-Sodium malonate pH 7 (3.4 M)
    294 μl of water (100 v/v)
    C4
    • Tacsimate 35 v/v -
    NA
    350 μl of Tacsimate pH 7 (100 v/v)
    650 μl of water (100 v/v)
    C5
    • Tacsimate 60 v/v -
    NA
    600 μl of Tacsimate pH 7 (100 v/v)
    400 μl of water (100 v/v)
    C6
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Ammonium sulfate 1.5 M -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    428 μl of Ammonium sulfate (3.5 M)
    20 μl of Sodium chloride (5 M)
    451 μl of water (100 v/v)
    C7
    • Potassium Sodium tartrate 0.8 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 5,000 0.5 w/v -
    pH 8.5
    10 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    533 μl of Potassium Sodium tartrate (1.5 M)
    356 μl of water (100 v/v)
    C8
    • Ammonium sulfate 1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 1 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    285 μl of Ammonium sulfate (3.5 M)
    20 μl of Polyethylene glycol 3,350 (50 w/v)
    594 μl of water (100 v/v)
    C9
    • di-Sodium malonate 1.1 M -
    • HEPES 0.1 M pH 7
    • Jeffamine® ED-2001 0.5 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    10 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
    323 μl of di-Sodium malonate pH 7 (3.4 M)
    566 μl of water (100 v/v)
    C10
    • di-Sodium succinate 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 1 w/v -
    pH 7
    833 μl of di-Sodium succinate (1.2 M)
    100 μl of HEPES pH 7 (1 M)
    20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    46 μl of water (100 v/v)
    C11
    • Ammonium sulfate 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 8,000 0.5 w/v -
    pH 7
    285 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7 (1 M)
    10 μl of Polyethylene glycol 8,000 (50 w/v)
    604 μl of water (100 v/v)
    C12
    • Tacsimate 15 v/v -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 3,350 2 w/v -
    pH 7
    40 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    150 μl of Tacsimate pH 7 (100 v/v)
    710 μl of water (100 v/v)
    D1
    • Polyethylene glycol 1,500 25 w/v -
    NA
    500 μl of Polyethylene glycol 1,500 (50 w/v)
    500 μl of water (100 v/v)
    D2
    • HEPES 0.1 M pH 7
    • Jeffamine® M-600 30 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Jeffamine® M-600 pH 7 (50 v/v)
    300 μl of water (100 v/v)
    D3
    • HEPES 0.1 M pH 7
    • Jeffamine® ED-2001 30 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    600 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
    300 μl of water (100 v/v)
    D4
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 3.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    400 μl of water (100 v/v)
    D5
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 4.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    D6
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D7
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D8
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    D9
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of water (100 v/v)
    D10
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 5,000 20 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    500 μl of water (100 v/v)
    D11
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 28 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    560 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    340 μl of water (100 v/v)
    D12
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Calcium chloride 0.2 M -
    pH 5.5
    100 μl of Calcium chloride (2 M)
    100 μl of BIS-TRIS pH 5.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    350 μl of water (100 v/v)
    E1
    • BIS-TRIS 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    • Calcium chloride 0.2 M -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    100 μl of Calcium chloride (2 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    350 μl of water (100 v/v)
    E2
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    E3
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    E4
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 7.5
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    E5
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 45 v/v -
    pH 8.5
    200 μl of Ammonium acetate (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    E6
    • Calcium chloride 0.05 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    25 μl of Calcium chloride (2 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    575 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.05 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    25 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    575 μl of water (100 v/v)
    E8
    • Potassium chloride 0.2 M -
    • HEPES 0.05 M pH 7.5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
    pH 7.5
    700 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    50 μl of HEPES pH 7.5 (1 M)
    50 μl of Potassium chloride (4 M)
    200 μl of water (100 v/v)
    E9
    • Ammonium sulfate 0.05 M -
    • BIS-TRIS 0.05 M pH 6.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
    pH 6.5
    50 μl of BIS-TRIS pH 6.5 (1 M)
    14 μl of Ammonium sulfate (3.5 M)
    600 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    335 μl of water (100 v/v)
    E10
    • BIS-TRIS 0.1 M pH 6.5
    • Polypropylene glycol 400 45 v/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    450 μl of Polypropylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    E11
    • Magnesium chloride 0.02 M -
    • HEPES 0.1 M pH 7.5
    • Poly(acrylic acid sodium salt) 5,100 22 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    10 μl of Magnesium chloride (2 M)
    440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
    450 μl of water (100 v/v)
    E12
    • Cobalt (II) chloride 0.01 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyvinylpyrrolidone K15 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyvinylpyrrolidone K15 (50 w/v)
    10 μl of Cobalt (II) chloride (1 M)
    490 μl of water (100 v/v)
    F1
    • L-Proline 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 10 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    200 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of L-Proline (1 M)
    500 μl of water (100 v/v)
    F2
    • Trimethylamine N-oxide 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 8.5
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Trimethylamine N-oxide (1 M)
    300 μl of water (100 v/v)
    F3
    • Tacsimate 5 v/v -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 5,000 10 w/v -
    pH 7
    200 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    50 μl of Tacsimate pH 7 (100 v/v)
    650 μl of water (100 v/v)
    F4
    • Cobalt (II) chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 12 w/v -
    • Nickel (II) chloride 0.005 M -
    • Cadmium chloride 0.005 M -
    • Magnesium chloride 0.005 M -
    pH 7.5
    1 μl of Nickel (II) chloride (4 M)
    100 μl of HEPES pH 7.5 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    2 μl of Magnesium chloride (2 M)
    5 μl of Cadmium chloride (1 M)
    5 μl of Cobalt (II) chloride (1 M)
    646 μl of water (100 v/v)
    F5
    • Ammonium acetate 0.1 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 10,000 17 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    100 μl of Ammonium acetate (1 M)
    340 μl of Polyethylene glycol 10,000 (50 w/v)
    460 μl of water (100 v/v)
    F6
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    342 μl of water (100 v/v)
    F7
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    342 μl of water (100 v/v)
    F8
    • Ammonium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    57 μl of Ammonium sulfate (3.5 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    342 μl of water (100 v/v)
    F9
    • Ammonium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    57 μl of Ammonium sulfate (3.5 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    342 μl of water (100 v/v)
    F10
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    F11
    • Sodium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    F12
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    G1
    • Sodium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    40 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    360 μl of water (100 v/v)
    G2
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    G3
    • Lithium sulfate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    G4
    • Lithium sulfate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    G5
    • Lithium sulfate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    G6
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    G7
    • Ammonium acetate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    G8
    • Ammonium acetate 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    200 μl of Ammonium acetate (1 M)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of water (100 v/v)
    G9
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    200 μl of Ammonium acetate (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of water (100 v/v)
    G10
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 5.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 5.5
    100 μl of BIS-TRIS pH 5.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    G11
    • Magnesium chloride 0.2 M -
    • BIS-TRIS 0.1 M pH 6.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 6.5
    100 μl of BIS-TRIS pH 6.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    G12
    • Magnesium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    300 μl of water (100 v/v)
    H1
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 3,350 25 w/v -
    pH 8.5
    500 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    H2
    • Potassium Sodium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of Potassium Sodium tartrate (1.5 M)
    466 μl of water (100 v/v)
    H3
    • di-Sodium malonate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    58 μl of di-Sodium malonate pH 7 (3.4 M)
    541 μl of water (100 v/v)
    H4
    • tri-Ammonium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    80 μl of tri-Ammonium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    H5
    • di-Sodium succinate 0.1 M -
    • Polyethylene glycol 3,350 15 w/v -
    NA
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    83 μl of di-Sodium succinate (1.2 M)
    616 μl of water (100 v/v)
    H6
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    28 μl of Sodium formate (7 M)
    571 μl of water (100 v/v)
    H7
    • di-Sodium DL-malate 0.15 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of di-Sodium DL-malate (3 M)
    550 μl of water (100 v/v)
    H8
    • Magnesium formate 0.1 M -
    • Polyethylene glycol 3,350 15 w/v -
    NA
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Magnesium formate (1 M)
    600 μl of water (100 v/v)
    H9
    • Zinc acetate 0.05 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Zinc acetate (1 M)
    550 μl of water (100 v/v)
    H10
    • tri-Sodium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    NA
    125 μl of tri-Sodium citrate (1.6 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    475 μl of water (100 v/v)
    H11
    • Potassium thiocyanate 0.1 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    NA
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    12 μl of Potassium thiocyanate (8 M)
    387 μl of water (100 v/v)
    H12
    • Potassium bromide 0.15 M -
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    NA
    37 μl of Potassium bromide (4 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    362 μl of water (100 v/v)