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Supplemental Information

Screen Description

Index Screen

Hampton Research / Index Screen
Created 2018-06-12.

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Index Screen

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Screen Structure

Index Screen

Well Count 96
Number of distinct ingredients 48
Internal diversity (score mean dist) 0.907

Screen Composition

2025-08-12T03:41:43.763983 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type Index Screen
Buffer 113
Complex ingredient 4
Organic (non-volatile) 8
Polymer 59
Salt 95

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
Index Screen Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.1 M pH 3.5
  • Ammonium sulfate 2 M -
pH 3.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
328.6 μl of water (100 v/v)
A2
  • Sodium acetate 0.1 M pH 4.5
  • Ammonium sulfate 2 M -
pH 4.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
328.6 μl of water (100 v/v)
A3
  • BIS-TRIS 0.1 M pH 5.5
  • Ammonium sulfate 2 M -
pH 5.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
328.6 μl of water (100 v/v)
A4
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
328.6 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 7.5
  • Ammonium sulfate 2 M -
pH 7.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
328.6 μl of water (100 v/v)
A6
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
A7
  • tri-Sodium citrate 0.1 M pH 3.5
  • Sodium chloride 3 M -
pH 3.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
300.0 μl of water (100 v/v)
A8
  • Sodium acetate 0.1 M pH 4.5
  • Sodium chloride 3 M -
pH 4.5
100.0 μl of Sodium acetate pH 4.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A9
  • BIS-TRIS 0.1 M pH 5.5
  • Sodium chloride 3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A10
  • BIS-TRIS 0.1 M pH 6.5
  • Sodium chloride 3 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A11
  • HEPES 0.1 M pH 7.5
  • Sodium chloride 3 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
600.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
A12
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium chloride 3 M -
pH 8.5
600.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
B1
  • BIS-TRIS 0.1 M pH 5.5
  • Magnesium formate 0.3 M -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
300.0 μl of Magnesium formate (1 M)
600.0 μl of water (100 v/v)
B2
  • BIS-TRIS 0.1 M pH 6.5
  • Magnesium formate 0.5 M -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Magnesium formate (1 M)
400.0 μl of water (100 v/v)
B3
  • HEPES 0.1 M pH 7.5
  • Magnesium formate 0.5 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Magnesium formate (1 M)
400.0 μl of water (100 v/v)
B4
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium formate 0.3 M -
pH 8.5
300.0 μl of Magnesium formate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
B5
  • Sodium di-hydrogen phosphate 1.26 M -
  • di-Potassium hydrogen phosphate 0.14 M -
NA
315.0 μl of Sodium di-hydrogen phosphate (4 M)
35.0 μl of di-Potassium hydrogen phosphate (4 M)
650.0 μl of water (100 v/v)
B6
  • Sodium di-hydrogen phosphate 0.49 M -
  • di-Potassium hydrogen phosphate 0.91 M -
NA
122.5 μl of Sodium di-hydrogen phosphate (4 M)
227.5 μl of di-Potassium hydrogen phosphate (4 M)
650.0 μl of water (100 v/v)
B7
  • Sodium di-hydrogen phosphate 0.056 M -
  • di-Potassium hydrogen phosphate 1.34 M -
NA
14.0 μl of Sodium di-hydrogen phosphate (4 M)
336.0 μl of di-Potassium hydrogen phosphate (4 M)
650.0 μl of water (100 v/v)
B8
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
875.0 μl of tri-Sodium citrate (1.6 M)
25.0 μl of water (100 v/v)
B9
  • tri-Ammonium citrate 1.8 M -
NA
720.0 μl of tri-Ammonium citrate (2.5 M)
280.0 μl of water (100 v/v)
B10
  • di-Sodium succinate 0.8 M -
NA
666.7 μl of di-Sodium succinate (1.2 M)
333.3 μl of water (100 v/v)
B11
  • di-Sodium DL-malate 2.1 M -
NA
700.0 μl of di-Sodium DL-malate (3 M)
300.0 μl of water (100 v/v)
B12
  • Sodium acetate 2.8 M -
NA
700.0 μl of Sodium acetate (4 M)
300.0 μl of water (100 v/v)
C1
  • Sodium formate 3.5 M -
NA
700.0 μl of Sodium formate (5 M)
300.0 μl of water (100 v/v)
C2
  • di-Ammonium tartrate 1.1 M -
NA
687.5 μl of di-Ammonium tartrate (1.6 M)
312.5 μl of water (100 v/v)
C3
  • di-Sodium malonate 2.4 M -
NA
705.9 μl of di-Sodium malonate pH 7 (3.4 M)
294.1 μl of water (100 v/v)
C4
  • Tacsimate 35 v/v -
NA
350.0 μl of Tacsimate pH 7 (100 v/v)
650.0 μl of water (100 v/v)
C5
  • Tacsimate 60 v/v -
NA
600.0 μl of Tacsimate pH 7 (100 v/v)
400.0 μl of water (100 v/v)
C6
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Ammonium sulfate 1.5 M -
pH 6.5
428.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
20.0 μl of Sodium chloride (5 M)
451.4 μl of water (100 v/v)
C7
  • Potassium Sodium tartrate 0.8 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 5,000 0.5 w/v -
pH 8.5
10.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
533.3 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
356.7 μl of water (100 v/v)
C8
  • Ammonium sulfate 1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 1 w/v -
pH 5.5
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
20.0 μl of Polyethylene glycol 3,350 (50 w/v)
594.3 μl of water (100 v/v)
C9
  • di-Sodium malonate 1.1 M -
  • HEPES 0.1 M pH 7
  • Jeffamine® ED-2001 0.5 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
323.5 μl of di-Sodium malonate pH 7 (3.4 M)
566.5 μl of water (100 v/v)
C10
  • di-Sodium succinate 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 1 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
833.3 μl of di-Sodium succinate (1.2 M)
46.7 μl of water (100 v/v)
C11
  • Ammonium sulfate 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 8,000 0.5 w/v -
pH 7
285.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7 (1 M)
10.0 μl of Polyethylene glycol 8,000 (50 w/v)
604.3 μl of water (100 v/v)
C12
  • Tacsimate 15 v/v -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 3,350 2 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
40.0 μl of Polyethylene glycol 3,350 (50 w/v)
150.0 μl of Tacsimate pH 7 (100 v/v)
710.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 1,500 25 w/v -
NA
500.0 μl of Polyethylene glycol 1,500 (50 w/v)
500.0 μl of water (100 v/v)
D2
  • HEPES 0.1 M pH 7
  • Jeffamine® M-600 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® M-600 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
D3
  • HEPES 0.1 M pH 7
  • Jeffamine® ED-2001 30 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
600.0 μl of Jeffamine® ED-2001 pH 7 (50 v/v)
300.0 μl of water (100 v/v)
D4
  • tri-Sodium citrate 0.1 M pH 3.5
  • Polyethylene glycol 3,350 25 w/v -
pH 3.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
400.0 μl of water (100 v/v)
D5
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 25 w/v -
pH 4.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
D6
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D7
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D8
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
D9
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
D10
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 5,000 20 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
500.0 μl of water (100 v/v)
D11
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 28 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
560.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
340.0 μl of water (100 v/v)
D12
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
E1
  • BIS-TRIS 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
  • Calcium chloride 0.2 M -
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Calcium chloride (2 M)
350.0 μl of water (100 v/v)
E2
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 5.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
250.0 μl of water (100 v/v)
E3
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 6.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
250.0 μl of water (100 v/v)
E4
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 7.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of water (100 v/v)
E5
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 45 v/v -
pH 8.5
450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
200.0 μl of Ammonium acetate (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
250.0 μl of water (100 v/v)
E6
  • Calcium chloride 0.05 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
25.0 μl of Calcium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
E7
  • Magnesium chloride 0.05 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
25.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
575.0 μl of water (100 v/v)
E8
  • Potassium chloride 0.2 M -
  • HEPES 0.05 M pH 7.5
  • Pentaerythritol Propoxylate (5/4 PO/OH) 35 v/v -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
700.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
50.0 μl of Potassium chloride (4 M)
200.0 μl of water (100 v/v)
E9
  • Ammonium sulfate 0.05 M -
  • BIS-TRIS 0.05 M pH 6.5
  • Pentaerythritol ethoxylate (15/4 EO/OH) 30 v/v -
pH 6.5
14.3 μl of Ammonium sulfate (3.5 M)
50.0 μl of BIS-TRIS pH 6.5 (1 M)
600.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
335.7 μl of water (100 v/v)
E10
  • BIS-TRIS 0.1 M pH 6.5
  • Polypropylene glycol 400 45 v/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
450.0 μl of Polypropylene glycol 400 (100 v/v)
450.0 μl of water (100 v/v)
E11
  • Magnesium chloride 0.02 M -
  • HEPES 0.1 M pH 7.5
  • Poly(acrylic acid sodium salt) 5,100 22 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
10.0 μl of Magnesium chloride (2 M)
440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v)
450.0 μl of water (100 v/v)
E12
  • Cobalt (II) chloride 0.01 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyvinylpyrrolidone K15 20 w/v -
pH 8.5
10.0 μl of Cobalt (II) chloride (1 M)
400.0 μl of Polyvinylpyrrolidone K15 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
490.0 μl of water (100 v/v)
F1
  • L-Proline 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 10 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of L-Proline (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F2
  • Trimethylamine N-oxide 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 8.5
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Trimethylamine N-oxide (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
F3
  • Tacsimate 5 v/v -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 5,000 10 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
50.0 μl of Tacsimate pH 7 (100 v/v)
650.0 μl of water (100 v/v)
F4
  • Cobalt (II) chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 12 w/v -
  • Nickel (II) chloride 0.005 M -
  • Cadmium chloride 0.005 M -
  • Magnesium chloride 0.005 M -
pH 7.5
5.0 μl of Cadmium chloride (1 M)
5.0 μl of Cobalt (II) chloride (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
2.5 μl of Magnesium chloride (2 M)
1.2 μl of Nickel (II) chloride (4 M)
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
646.2 μl of water (100 v/v)
F5
  • Ammonium acetate 0.1 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 10,000 17 w/v -
pH 5.5
100.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
340.0 μl of Polyethylene glycol 10,000 (50 w/v)
460.0 μl of water (100 v/v)
F6
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F7
  • Ammonium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F8
  • Ammonium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
57.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
342.9 μl of water (100 v/v)
F9
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
57.1 μl of Ammonium sulfate (3.5 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
342.9 μl of water (100 v/v)
F10
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
F11
  • Sodium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
F12
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
360.0 μl of water (100 v/v)
G1
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
360.0 μl of water (100 v/v)
G2
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G3
  • Lithium sulfate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G4
  • Lithium sulfate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G5
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
G6
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G7
  • Ammonium acetate 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of BIS-TRIS pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G8
  • Ammonium acetate 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
200.0 μl of Ammonium acetate (1 M)
100.0 μl of HEPES pH 7.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
200.0 μl of water (100 v/v)
G9
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
200.0 μl of Ammonium acetate (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
200.0 μl of water (100 v/v)
G10
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G11
  • Magnesium chloride 0.2 M -
  • BIS-TRIS 0.1 M pH 6.5
  • Polyethylene glycol 3,350 25 w/v -
pH 6.5
100.0 μl of BIS-TRIS pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
G12
  • Magnesium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,350 25 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
H1
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 3,350 25 w/v -
pH 8.5
100.0 μl of Magnesium chloride (2 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
300.0 μl of water (100 v/v)
H2
  • Potassium Sodium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of Potassium Sodium tartrate (1.5 M)
466.7 μl of water (100 v/v)
H3
  • di-Sodium malonate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
58.8 μl of di-Sodium malonate pH 7 (3.4 M)
541.2 μl of water (100 v/v)
H4
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
520.0 μl of water (100 v/v)
H5
  • di-Sodium succinate 0.1 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
83.3 μl of di-Sodium succinate (1.2 M)
616.7 μl of water (100 v/v)
H6
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium formate (7 M)
571.4 μl of water (100 v/v)
H7
  • di-Sodium DL-malate 0.15 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of di-Sodium DL-malate (3 M)
550.0 μl of water (100 v/v)
H8
  • Magnesium formate 0.1 M -
  • Polyethylene glycol 3,350 15 w/v -
NA
100.0 μl of Magnesium formate (1 M)
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
600.0 μl of water (100 v/v)
H9
  • Zinc acetate 0.05 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Zinc acetate (1 M)
550.0 μl of water (100 v/v)
H10
  • tri-Sodium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
125.0 μl of tri-Sodium citrate (1.6 M)
475.0 μl of water (100 v/v)
H11
  • Potassium thiocyanate 0.1 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
12.5 μl of Potassium thiocyanate (8 M)
387.5 μl of water (100 v/v)
H12
  • Potassium bromide 0.15 M -
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
NA
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
37.5 μl of Potassium bromide (4 M)
362.5 μl of water (100 v/v)