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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG+ Eco
Barcodes
JCSG+ Eco
No barcodes found.
Screen Structure
JCSG+ Eco
Well Count | 96 |
---|---|
Number of distinct ingredients | 68 |
Internal diversity (score mean dist) | 0.960 |
Screen Composition
ingredient type | JCSG+ Eco |
---|---|
Buffer | 102 |
Cryoprotectant | 6 |
Organic (non-volatile) | 25 |
Organic (volatile) | 5 |
Polymer | 65 |
Salt | 90 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 400 (100 v/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 300.0 μl of water (100 v/v) |
A2 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 22.7 μl of tri-Sodium citrate pH 3 (1.6 M) 39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M) 537.5 μl of water (100 v/v) |
A3 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of di-Ammonium hydrogen citrate (2.5 M) 520.0 μl of water (100 v/v) |
A4 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10.0 μl of Calcium chloride (2 M) 47.5 μl of Sodium acetate pH 4 (1 M) 52.5 μl of Sodium acetate pH 5 (1 M) 590.0 μl of water (100 v/v) |
A5 |
| NA | 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
A6 |
| pH 4.2 | 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 400.0 μl of water (100 v/v) |
A7 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
A8 |
| NA | 20.0 μl of Ammonium formate (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
A9 |
| NA | 40.0 μl of Ammonium chloride (5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 560.0 μl of water (100 v/v) |
A10 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 14.3 μl of Potassium formate (14 M) 585.7 μl of water (100 v/v) |
A11 |
| pH 8.5 | 500.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v) |
A12 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of Potassium nitrate (3 M) 533.3 μl of water (100 v/v) |
B1 |
| pH 4 | 228.6 μl of Ammonium sulfate (3.5 M) 39.4 μl of tri-Sodium citrate pH 3 (1.6 M) 23.1 μl of tri-Sodium citrate pH 6.5 (1.6 M) 708.9 μl of water (100 v/v) |
B2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Sodium thiocyanate (8 M) 575.0 μl of water (100 v/v) |
B3 |
| pH 9 | 9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 80.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 620.0 μl of water (100 v/v) |
B5 |
| pH 6.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v) |
B6 |
| pH 4.2 | 400.0 μl of Ethanol (100 v/v) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 400.0 μl of water (100 v/v) |
B7 |
| pH 4.6 | 160.0 μl of Polyethylene glycol 4,000 (50 w/v) 47.5 μl of Sodium acetate pH 4 (1 M) 52.5 μl of Sodium acetate pH 5 (1 M) 740.0 μl of water (100 v/v) |
B8 |
| pH 7 | 100.0 μl of Magnesium chloride (2 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 600.0 μl of water (100 v/v) |
B9 |
| pH 5 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 29.9 μl of tri-Sodium citrate pH 3 (1.6 M) 32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M) 537.5 μl of water (100 v/v) |
B10 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 200 (100 v/v) 300.0 μl of water (100 v/v) |
B11 |
| NA | 1000.0 μl of tri-Sodium citrate (1.6 M) |
B12 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Potassium citrate (2.5 M) 520.0 μl of water (100 v/v) |
C1 |
| pH 4.2 | 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 40.0 μl of Sodium chloride (5 M) 460.0 μl of water (100 v/v) |
C2 |
| pH 4 | 100.0 μl of Lithium chloride (10 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 39.4 μl of tri-Sodium citrate pH 3 (1.6 M) 23.1 μl of tri-Sodium citrate pH 6.5 (1.6 M) 437.5 μl of water (100 v/v) |
C3 |
| NA | 100.0 μl of Ammonium nitrate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
C4 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
C5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Potassium di-hydrogen phosphate (2 M) 160.0 μl of Sodium di-hydrogen phosphate (5 M) 340.0 μl of water (100 v/v) |
C6 |
| pH 4.2 | 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Potassium phosphate citrate pH 4.2 (1 M) 500.0 μl of water (100 v/v) |
C7 |
| pH 4.5 | 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 200.0 μl of Zinc acetate (1 M) 500.0 μl of water (100 v/v) |
C8 |
| pH 8.5 | 200.0 μl of Ethanol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v) |
C9 |
| pH 6.2 | 250.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Glycerol (100 v/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 616.7 μl of water (100 v/v) |
C10 |
| pH 9 | 9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 333.3 μl of Polyethylene glycol 20,000 (30 w/v) 566.7 μl of water (100 v/v) |
C11 |
| pH 4.6 | 571.4 μl of Ammonium sulfate (3.5 M) 47.5 μl of Sodium acetate pH 4 (1 M) 52.5 μl of Sodium acetate pH 5 (1 M) 328.6 μl of water (100 v/v) |
C12 |
| NA | 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 600.0 μl of water (100 v/v) |
D1 |
| NA | 200.0 μl of Glycerol (100 v/v) 480.0 μl of Polyethylene glycol 1,500 (50 w/v) 320.0 μl of water (100 v/v) |
D2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 500.0 μl of water (100 v/v) |
D3 |
| pH 6.2 | 500.0 μl of Polyethylene glycol 200 (100 v/v) 33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40.0 μl of Sodium chloride (5 M) 426.7 μl of water (100 v/v) |
D4 |
| pH 4.5 | 100.0 μl of Lithium sulfate (2 M) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 200.0 μl of water (100 v/v) |
D5 |
| pH 7.5 | 700.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) -0.0 μl of HEPES pH 5 (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of water (100 v/v) |
D6 |
| pH 8.5 | 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D7 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
D8 |
| pH 8 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 53.0 μl of tris chloride BCC pH 7.5 (1 M) 47.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
D9 |
| NA | 48.6 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 510.0 μl of Polyethylene glycol 4,000 (50 w/v) 291.4 μl of water (100 v/v) |
D10 |
| pH 6.5 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 300.0 μl of water (100 v/v) |
D11 |
| pH 4.6 | 140.0 μl of 2-Propanol (100 v/v) 70.0 μl of Calcium chloride (2 M) 300.0 μl of Glycerol (100 v/v) 33.3 μl of Sodium acetate pH 4 (1 M) 36.7 μl of Sodium acetate pH 5 (1 M) 420.0 μl of water (100 v/v) |
D12 |
| NA | 200.0 μl of Glycerol (100 v/v) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 20.0 μl of Potassium di-hydrogen phosphate (2 M) 460.0 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v) |
E2 |
| pH 6.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 6.5 (1 M) 40.0 μl of Sodium chloride (5 M) 288.6 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 40.0 μl of Sodium chloride (5 M) 760.0 μl of water (100 v/v) |
E4 |
| pH 8.5 | 360.0 μl of Ammonium sulfate (3.5 M) 100.0 μl of Lithium sulfate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 440.0 μl of water (100 v/v) |
E5 |
| pH 10 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50.0 μl of CAPS pH 9.5 (1 M) 50.0 μl of CAPS pH 12 (1 M) 500.0 μl of water (100 v/v) |
E6 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 200.0 μl of Zinc acetate (1 M) 300.0 μl of water (100 v/v) |
E7 |
| pH 6.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Zinc acetate (1 M) 600.0 μl of water (100 v/v) |
E8 |
| pH 4.5 | 400.0 μl of Ammonium di-hydrogen phosphate (2.5 M) 58.6 μl of Sodium acetate pH 4 (1 M) 41.4 μl of Sodium acetate pH 5 (1 M) 500.0 μl of water (100 v/v) |
E9 |
| pH 6.5 | 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 640.0 μl of Magnesium sulfate (2.5 M) 160.0 μl of water (100 v/v) |
E10 |
| pH 9 | 9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 700.0 μl of water (100 v/v) |
E11 |
| pH 6.5 | 160.0 μl of Calcium acetate (1 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of MES pH 6.5 (1 M) 288.0 μl of Polyethylene glycol 8,000 (50 w/v) 272.0 μl of water (100 v/v) |
E12 |
| pH 8 | 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 700.0 μl of water (100 v/v) |
F1 |
| pH 6.5 | 50.0 μl of Cesium chloride (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 48.6 μl of MES pH 5.2 (0.5 M) 151.4 μl of MES pH 7.1 (0.5 M) 150.0 μl of water (100 v/v) |
F2 |
| pH 5 | 914.3 μl of Ammonium sulfate (3.5 M) 29.9 μl of tri-Sodium citrate pH 3 (1.6 M) 32.6 μl of tri-Sodium citrate pH 6.5 (1.6 M) 23.2 μl of water (100 v/v) |
F3 |
| pH 8 | 200.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 53.0 μl of tris chloride BCC pH 7.5 (1 M) 47.0 μl of tris chloride BCC pH 8.5 (1 M) 700.0 μl of water (100 v/v) |
F4 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 500.0 μl of water (100 v/v) |
F5 |
| pH 8.5 | 500.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium chloride (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
F6 |
| pH 9 | 100.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 9.5 μl of BICINE pH 7.4 (1 M) 90.5 μl of BICINE pH 9.3 (1 M) 800.0 μl of water (100 v/v) |
F7 |
| NA | 266.7 μl of di-Sodium succinate pH 7 (3 M) 733.3 μl of water (100 v/v) |
F8 |
| NA | 700.0 μl of di-Sodium DL-malate pH 7 (3 M) 300.0 μl of water (100 v/v) |
F9 |
| NA | 800.0 μl of di-Sodium malonate pH 7 (3 M) 200.0 μl of water (100 v/v) |
F10 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 10.0 μl of Jeffamine® ED-2003 pH 7 (50 v/v) 366.7 μl of di-Sodium malonate pH 7 (3 M) 523.3 μl of water (100 v/v) |
F11 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 20.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 333.3 μl of di-Sodium succinate pH 7 (3 M) 546.7 μl of water (100 v/v) |
F12 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® M-600 pH 7 (50 v/v) 300.0 μl of water (100 v/v) |
G1 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 600.0 μl of Jeffamine® ED-2003 pH 7 (50 v/v) 300.0 μl of water (100 v/v) |
G2 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 10.0 μl of Magnesium chloride (2 M) 440.0 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 450.0 μl of water (100 v/v) |
G3 |
| pH 8.5 | 10.0 μl of Cobalt (II) chloride (1 M) 400.0 μl of Polyvinylpyrrolidone K15 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 490.0 μl of water (100 v/v) |
G4 |
| pH 8.5 | 400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200.0 μl of Trimethylamine N-oxide (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
G5 |
| pH 7.5 | 5.0 μl of Cadmium chloride (1 M) 5.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 2.5 μl of Magnesium chloride (2 M) 1.2 μl of Nickel (II) chloride (4 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 646.2 μl of water (100 v/v) |
G6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 66.7 μl of di-Sodium malonate pH 7 (3 M) 533.3 μl of water (100 v/v) |
G7 |
| NA | 300.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.3 μl of di-Sodium succinate pH 7 (3 M) 666.7 μl of water (100 v/v) |
G8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of di-Sodium DL-malate pH 7 (3 M) 550.0 μl of water (100 v/v) |
G9 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12.5 μl of Potassium thiocyanate (8 M) 387.5 μl of water (100 v/v) |
G10 |
| NA | 600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 37.5 μl of Potassium bromide (4 M) 362.5 μl of water (100 v/v) |
G11 |
| pH 5.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 328.6 μl of water (100 v/v) |
G12 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 600.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
H1 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 300.0 μl of Magnesium formate (1 M) 600.0 μl of water (100 v/v) |
H2 |
| pH 5.5 | 285.7 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 20.0 μl of Polyethylene glycol 3,350 (50 w/v) 594.3 μl of water (100 v/v) |
H3 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
H4 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Calcium chloride (2 M) 350.0 μl of water (100 v/v) |
H5 |
| pH 5.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 250.0 μl of water (100 v/v) |
H6 |
| pH 5.5 | 100.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 340.0 μl of Polyethylene glycol 10,000 (50 w/v) 460.0 μl of water (100 v/v) |
H7 |
| pH 5.5 | 57.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 342.9 μl of water (100 v/v) |
H8 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 40.0 μl of Sodium chloride (5 M) 360.0 μl of water (100 v/v) |
H9 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
H10 |
| pH 5.5 | 200.0 μl of Ammonium acetate (1 M) 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 200.0 μl of water (100 v/v) |
H11 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v) |
H12 |
| pH 7.5 | 450.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200.0 μl of Ammonium acetate (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 250.0 μl of water (100 v/v) |