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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG+ Eco
Barcodes
JCSG+ Eco
Screen Structure
JCSG+ Eco
Well Count | 96 |
---|---|
Number of distinct ingredients | 68 |
Internal diversity (score mean dist) | 0.960 |
Screen Composition
ingredient type | JCSG+ Eco |
---|---|
Buffer | 102 |
Cryoprotectant | 6 |
Organic (non-volatile) | 25 |
Organic (volatile) | 5 |
Polymer | 65 |
Salt | 90 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 100 μl of Lithium sulfate (2 M) 41 μl of Sodium acetate pH 5 (1 M) 58 μl of Sodium acetate pH 4 (1 M) 500 μl of Polyethylene glycol 400 (100 v/v) 300 μl of water (100 v/v) |
A2 |
| pH 5.5 | 58 μl of tri-Sodium citrate pH 6.5 (1.6 M) 3 μl of tri-Sodium citrate pH 3 (1.6 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 537 μl of water (100 v/v) |
A3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of di-Ammonium hydrogen citrate (2.5 M) 520 μl of water (100 v/v) |
A4 |
| pH 4.6 | 300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 10 μl of Calcium chloride (2 M) 52 μl of Sodium acetate pH 5 (1 M) 47 μl of Sodium acetate pH 4 (1 M) 590 μl of water (100 v/v) |
A5 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 200 μl of Magnesium formate (1 M) 400 μl of water (100 v/v) |
A6 |
| pH 4.2 | 100 μl of Lithium sulfate (2 M) 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
A7 |
| pH 9.5 | 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of CHES pH 9.5 (1 M) 500 μl of water (100 v/v) |
A8 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 20 μl of Ammonium formate (10 M) 580 μl of water (100 v/v) |
A9 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Ammonium chloride (5 M) 560 μl of water (100 v/v) |
A10 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 14 μl of Potassium formate (14 M) 585 μl of water (100 v/v) |
A11 |
| pH 8.5 | 500 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 80 μl of Ammonium di-hydrogen phosphate (2.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 320 μl of water (100 v/v) |
A12 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of Potassium nitrate (3 M) 533 μl of water (100 v/v) |
B1 |
| pH 4 | 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 10 μl of tri-Sodium citrate pH 3 (1.6 M) 228 μl of Ammonium sulfate (3.5 M) 708 μl of water (100 v/v) |
B2 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 25 μl of Sodium thiocyanate (8 M) 575 μl of water (100 v/v) |
B3 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 9 μl of BICINE pH 7.4 (1 M) 500 μl of water (100 v/v) |
B4 |
| pH 7.5 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 80 μl of Ethylene glycol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 620 μl of water (100 v/v) |
B5 |
| pH 6.5 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of MES pH 6.5 (1 M) 400 μl of water (100 v/v) |
B6 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Ethanol (100 v/v) 100 μl of Polyethylene glycol 1,000 (50 w/v) 400 μl of water (100 v/v) |
B7 |
| pH 4.6 | 52 μl of Sodium acetate pH 5 (1 M) 47 μl of Sodium acetate pH 4 (1 M) 160 μl of Polyethylene glycol 4,000 (50 w/v) 740 μl of water (100 v/v) |
B8 |
| pH 7 | 200 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 7 (1 M) 100 μl of Magnesium chloride (2 M) 600 μl of water (100 v/v) |
B9 |
| pH 5 | 56 μl of tri-Sodium citrate pH 6.5 (1.6 M) 5 μl of tri-Sodium citrate pH 3 (1.6 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 537 μl of water (100 v/v) |
B10 |
| pH 6.5 | 100 μl of MES pH 6.5 (1 M) 100 μl of Magnesium chloride (2 M) 500 μl of Polyethylene glycol 200 (100 v/v) 300 μl of water (100 v/v) |
B11 |
| NA | 1000 μl of tri-Sodium citrate (1.6 M) |
B12 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 80 μl of tri-Potassium citrate (2.5 M) 520 μl of water (100 v/v) |
C1 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 40 μl of Sodium chloride (5 M) 400 μl of Polyethylene glycol 8,000 (50 w/v) 460 μl of water (100 v/v) |
C2 |
| pH 4 | 51 μl of tri-Sodium citrate pH 6.5 (1.6 M) 10 μl of tri-Sodium citrate pH 3 (1.6 M) 400 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of Lithium chloride (10 M) 437 μl of water (100 v/v) |
C3 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of Ammonium nitrate (2 M) 500 μl of water (100 v/v) |
C4 |
| pH 7 | 200 μl of Polyethylene glycol 6,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 700 μl of water (100 v/v) |
C5 |
| pH 7.5 | 400 μl of Potassium di-hydrogen phosphate (2 M) 100 μl of HEPES pH 7.5 (1 M) 160 μl of Sodium di-hydrogen phosphate (5 M) 340 μl of water (100 v/v) |
C6 |
| pH 4.2 | 100 μl of Potassium phosphate citrate pH 4.2 (1 M) 400 μl of Polyethylene glycol 300 (100 v/v) 500 μl of water (100 v/v) |
C7 |
| pH 4.5 | 41 μl of Sodium acetate pH 5 (1 M) 58 μl of Sodium acetate pH 4 (1 M) 200 μl of Polyethylene glycol 3,000 (50 w/v) 200 μl of Zinc acetate (1 M) 500 μl of water (100 v/v) |
C8 |
| pH 8.5 | 200 μl of Ethanol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 700 μl of water (100 v/v) |
C9 |
| pH 6.2 | 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 100 μl of Glycerol (100 v/v) 250 μl of 1,2-Propanediol (100 v/v) 616 μl of water (100 v/v) |
C10 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 333 μl of Polyethylene glycol 20,000 (30 w/v) 9 μl of BICINE pH 7.4 (1 M) 566 μl of water (100 v/v) |
C11 |
| pH 4.6 | 52 μl of Sodium acetate pH 5 (1 M) 47 μl of Sodium acetate pH 4 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v) |
C12 |
| NA | 200 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Polyethylene glycol 1,000 (50 w/v) 600 μl of water (100 v/v) |
D1 |
| NA | 200 μl of Glycerol (100 v/v) 480 μl of Polyethylene glycol 1,500 (50 w/v) 320 μl of water (100 v/v) |
D2 |
| pH 7.5 | 300 μl of Polyethylene glycol 400 (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Magnesium chloride (2 M) 500 μl of water (100 v/v) |
D3 |
| pH 6.2 | 33 μl of Sodium Potassium phosphate pH 6.2 (3 M) 40 μl of Sodium chloride (5 M) 500 μl of Polyethylene glycol 200 (100 v/v) 426 μl of water (100 v/v) |
D4 |
| pH 4.5 | 100 μl of Lithium sulfate (2 M) 41 μl of Sodium acetate pH 5 (1 M) 58 μl of Sodium acetate pH 4 (1 M) 600 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of water (100 v/v) |
D5 |
| pH 7.5 | 700 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 0 μl of HEPES pH 5 (1 M) 100 μl of HEPES pH 7.5 (1 M) 200 μl of water (100 v/v) |
D6 |
| pH 8.5 | 400 μl of Polyethylene glycol 8,000 (50 w/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 400 μl of water (100 v/v) |
D7 |
| pH 8.5 | 100 μl of Lithium sulfate (2 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol 400 (100 v/v) 400 μl of water (100 v/v) |
D8 |
| pH 8 | 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 47 μl of tris chloride BCC pH 8.5 (1 M) 53 μl of tris chloride BCC pH 7.5 (1 M) 500 μl of water (100 v/v) |
D9 |
| NA | 48 μl of Ammonium sulfate (3.5 M) 150 μl of Glycerol (100 v/v) 510 μl of Polyethylene glycol 4,000 (50 w/v) 291 μl of water (100 v/v) |
D10 |
| pH 6.5 | 400 μl of Polyethylene glycol 300 (100 v/v) 200 μl of Calcium acetate (1 M) 100 μl of MES pH 6.5 (1 M) 300 μl of water (100 v/v) |
D11 |
| pH 4.6 | 70 μl of Calcium chloride (2 M) 36 μl of Sodium acetate pH 5 (1 M) 33 μl of Sodium acetate pH 4 (1 M) 300 μl of Glycerol (100 v/v) 140 μl of 2-Propanol (100 v/v) 420 μl of water (100 v/v) |
D12 |
| NA | 320 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 20 μl of Potassium di-hydrogen phosphate (2 M) 460 μl of water (100 v/v) |
E1 |
| pH 6.5 | 625 μl of tri-Sodium citrate (1.6 M) 100 μl of MES pH 6.5 (1 M) 275 μl of water (100 v/v) |
E2 |
| pH 6.5 | 40 μl of Sodium chloride (5 M) 571 μl of Ammonium sulfate (3.5 M) 100 μl of MES pH 6.5 (1 M) 288 μl of water (100 v/v) |
E3 |
| pH 7.5 | 40 μl of Sodium chloride (5 M) 100 μl of 2-Propanol (100 v/v) 100 μl of HEPES pH 7.5 (1 M) 760 μl of water (100 v/v) |
E4 |
| pH 8.5 | 100 μl of Lithium sulfate (2 M) 360 μl of Ammonium sulfate (3.5 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 440 μl of water (100 v/v) |
E5 |
| pH 10 | 50 μl of CAPS pH 12 (1 M) 400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 50 μl of CAPS pH 9.5 (1 M) 500 μl of water (100 v/v) |
E6 |
| pH 8 | 100 μl of Imidazole pH 8 (1 M) 400 μl of Polyethylene glycol 3,000 (50 w/v) 200 μl of Zinc acetate (1 M) 300 μl of water (100 v/v) |
E7 |
| pH 6.5 | 200 μl of Zinc acetate (1 M) 100 μl of 2-Propanol (100 v/v) 100 μl of MES pH 6.5 (1 M) 600 μl of water (100 v/v) |
E8 |
| pH 4.5 | 41 μl of Sodium acetate pH 5 (1 M) 58 μl of Sodium acetate pH 4 (1 M) 400 μl of Ammonium di-hydrogen phosphate (2.5 M) 500 μl of water (100 v/v) |
E9 |
| pH 6.5 | 48 μl of MES pH 5.2 (0.5 M) 151 μl of MES pH 7.1 (0.5 M) 640 μl of Magnesium sulfate (2.5 M) 160 μl of water (100 v/v) |
E10 |
| pH 9 | 90 μl of BICINE pH 9.3 (1 M) 200 μl of Polyethylene glycol 6,000 (50 w/v) 9 μl of BICINE pH 7.4 (1 M) 700 μl of water (100 v/v) |
E11 |
| pH 6.5 | 288 μl of Polyethylene glycol 8,000 (50 w/v) 200 μl of Glycerol (100 v/v) 160 μl of Calcium acetate (1 M) 80 μl of MES pH 6.5 (1 M) 272 μl of water (100 v/v) |
E12 |
| pH 8 | 100 μl of Imidazole pH 8 (1 M) 200 μl of Polyethylene glycol 8,000 (50 w/v) 700 μl of water (100 v/v) |
F1 |
| pH 6.5 | 48 μl of MES pH 5.2 (0.5 M) 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 50 μl of Cesium chloride (1 M) 151 μl of MES pH 7.1 (0.5 M) 150 μl of water (100 v/v) |
F2 |
| pH 5 | 56 μl of tri-Sodium citrate pH 6.5 (1.6 M) 5 μl of tri-Sodium citrate pH 3 (1.6 M) 914 μl of Ammonium sulfate (3.5 M) 23 μl of water (100 v/v) |
F3 |
| pH 8 | 200 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 47 μl of tris chloride BCC pH 8.5 (1 M) 53 μl of tris chloride BCC pH 7.5 (1 M) 700 μl of water (100 v/v) |
F4 |
| pH 7.5 | 400 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v) |
F5 |
| pH 8.5 | 500 μl of Ethylene glycol (100 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 100 μl of Magnesium chloride (2 M) 300 μl of water (100 v/v) |
F6 |
| pH 9 | 100 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 90 μl of BICINE pH 9.3 (1 M) 9 μl of BICINE pH 7.4 (1 M) 800 μl of water (100 v/v) |
F7 |
| NA | 266 μl of di-Sodium succinate pH 7 (3 M) 733 μl of water (100 v/v) |
F8 |
| NA | 700 μl of di-Sodium DL-malate pH 7 (3 M) 300 μl of water (100 v/v) |
F9 |
| NA | 800 μl of di-Sodium malonate pH 7 (3 M) 200 μl of water (100 v/v) |
F10 |
| pH 7 | 366 μl of di-Sodium malonate pH 7 (3 M) 10 μl of Jeffamine® ED-2003 pH 7 (50 v/v) 100 μl of HEPES pH 7 (1 M) 523 μl of water (100 v/v) |
F11 |
| pH 7 | 333 μl of di-Sodium succinate pH 7 (3 M) 20 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 100 μl of HEPES pH 7 (1 M) 546 μl of water (100 v/v) |
F12 |
| pH 7 | 600 μl of Jeffamine® M-600 pH 7 (50 v/v) 100 μl of HEPES pH 7 (1 M) 300 μl of water (100 v/v) |
G1 |
| pH 7 | 600 μl of Jeffamine® ED-2003 pH 7 (50 v/v) 100 μl of HEPES pH 7 (1 M) 300 μl of water (100 v/v) |
G2 |
| pH 7.5 | 440 μl of Poly(acrylic acid sodium salt) 5,100 (50 w/v) 100 μl of HEPES pH 7.5 (1 M) 10 μl of Magnesium chloride (2 M) 450 μl of water (100 v/v) |
G3 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 10 μl of Cobalt (II) chloride (1 M) 400 μl of Polyvinylpyrrolidone K15 (50 w/v) 490 μl of water (100 v/v) |
G4 |
| pH 8.5 | 100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 200 μl of Trimethylamine N-oxide (1 M) 300 μl of water (100 v/v) |
G5 |
| pH 7.5 | 240 μl of Polyethylene glycol 3,350 (50 w/v) 5 μl of Cadmium chloride (1 M) 5 μl of Cobalt (II) chloride (1 M) 100 μl of HEPES pH 7.5 (1 M) 2 μl of Magnesium chloride (2 M) 1 μl of Nickel (II) chloride (4 M) 646 μl of water (100 v/v) |
G6 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 66 μl of di-Sodium malonate pH 7 (3 M) 533 μl of water (100 v/v) |
G7 |
| NA | 300 μl of Polyethylene glycol 3,350 (50 w/v) 33 μl of di-Sodium succinate pH 7 (3 M) 666 μl of water (100 v/v) |
G8 |
| NA | 400 μl of Polyethylene glycol 3,350 (50 w/v) 50 μl of di-Sodium DL-malate pH 7 (3 M) 550 μl of water (100 v/v) |
G9 |
| NA | 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 12 μl of Potassium thiocyanate (8 M) 387 μl of water (100 v/v) |
G10 |
| NA | 37 μl of Potassium bromide (4 M) 600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v) 362 μl of water (100 v/v) |
G11 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 571 μl of Ammonium sulfate (3.5 M) 328 μl of water (100 v/v) |
G12 |
| pH 5.5 | 600 μl of Sodium chloride (5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of water (100 v/v) |
H1 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of Magnesium formate (1 M) 600 μl of water (100 v/v) |
H2 |
| pH 5.5 | 20 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 285 μl of Ammonium sulfate (3.5 M) 594 μl of water (100 v/v) |
H3 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 400 μl of water (100 v/v) |
H4 |
| pH 5.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of Calcium chloride (2 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 350 μl of water (100 v/v) |
H5 |
| pH 5.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 250 μl of water (100 v/v) |
H6 |
| pH 5.5 | 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Ammonium acetate (1 M) 340 μl of Polyethylene glycol 10,000 (50 w/v) 460 μl of water (100 v/v) |
H7 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 57 μl of Ammonium sulfate (3.5 M) 342 μl of water (100 v/v) |
H8 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 40 μl of Sodium chloride (5 M) 100 μl of BIS-TRIS pH 5.5 (1 M) 360 μl of water (100 v/v) |
H9 |
| pH 5.5 | 100 μl of Lithium sulfate (2 M) 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 300 μl of water (100 v/v) |
H10 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 200 μl of Ammonium acetate (1 M) 200 μl of water (100 v/v) |
H11 |
| pH 5.5 | 500 μl of Polyethylene glycol 3,350 (50 w/v) 100 μl of BIS-TRIS pH 5.5 (1 M) 100 μl of Magnesium chloride (2 M) 300 μl of water (100 v/v) |
H12 |
| pH 7.5 | 450 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 200 μl of Ammonium acetate (1 M) 100 μl of HEPES pH 7.5 (1 M) 250 μl of water (100 v/v) |