C6 - Screen Contents

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Supplemental Information

Screen Description

BCS

Molecular Dimensions / BCS

Created 2018-08-03.

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BCS

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BCS

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Screen Structure

BCS

Well Count	96
Number of distinct ingredients	47
Internal diversity (score mean dist)	0.929

Screen Composition

More information about screen composition chart.

ingredient type	BCS
Buffer	117
Complex ingredient	96
Cryoprotectant	7
Organic (non-volatile)	18
Organic (volatile)	3
Salt	124

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

BCS Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.1 M pH 4.5 PEG Smear Low 30 v/v - 🔍	pH 4.5	600.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 300.0 μl of water (100 v/v)
A2	Sodium phosphate citrate 0.1 M pH 5.5 PEG Smear Low 30 v/v - 🔍	pH 5.5	600.0 μl of PEG Smear Low (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 300.0 μl of water (100 v/v)
A3	MES 0.1 M pH 6.5 PEG Smear Low 30 v/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v)
A4	PEG Smear Medium 25 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 400.0 μl of water (100 v/v)
A5	PEG Smear Medium 25 v/v - Sodium phosphate citrate 0.1 M pH 5.5 🔍	pH 5.5	500.0 μl of PEG Smear Medium (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 400.0 μl of water (100 v/v)
A6	PEG Smear Medium 25 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v)
A7	Sodium acetate 0.1 M pH 4.5 PEG Smear High 20 v/v - 🔍	pH 4.5	400.0 μl of PEG Smear High (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of water (100 v/v)
A8	Sodium phosphate citrate 0.1 M pH 5.5 PEG Smear High 20 v/v - 🔍	pH 5.5	400.0 μl of PEG Smear High (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 500.0 μl of water (100 v/v)
A9	MES 0.1 M pH 6.5 PEG Smear High 20 v/v - 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v)
A10	PEG Smear Broad 22 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	440.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 460.0 μl of water (100 v/v)
A11	PEG Smear Broad 22 v/v - Sodium phosphate citrate 0.1 M pH 5.5 🔍	pH 5.5	440.0 μl of PEG Smear Broad (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 460.0 μl of water (100 v/v)
A12	PEG Smear Broad 22 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v)
B1	PEG Smear Low 30 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v)
B2	PEG Smear Low 30 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	600.0 μl of PEG Smear Low (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v)
B3	PEG Smear Low 30 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100.0 μl of BICINE pH 9.3 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v)
B4	PEG Smear Medium 25 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v)
B5	PEG Smear Medium 25 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
B6	PEG Smear Medium 25 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100.0 μl of BICINE pH 9.3 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v)
B7	PEG Smear High 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v)
B8	PEG Smear High 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	400.0 μl of PEG Smear High (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
B9	PEG Smear High 20 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100.0 μl of BICINE pH 9.3 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v)
B10	PEG Smear Broad 22 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v)
B11	PEG Smear Broad 22 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	440.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 460.0 μl of water (100 v/v)
B12	PEG Smear Broad 22 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100.0 μl of BICINE pH 9.3 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v)
C1	PEG Smear Low 35 v/v - 🔍	NA	700.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v)
C2	PEG Smear Low 28 v/v - Ethylene glycol 5 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium acetate 0.2 M - 🔍	pH 4.6	66.7 μl of Ammonium acetate (3 M) 166.7 μl of Ethylene glycol (30 v/v) 560.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 106.7 μl of water (100 v/v)
C3	PEG Smear Medium 28 v/v - Sodium chloride 0.15 M - 🔍	NA	560.0 μl of PEG Smear Medium (50 v/v) 75.0 μl of Sodium chloride (2 M) 365.0 μl of water (100 v/v)
C4	PEG Smear Medium 25 v/v - Ammonium sulfate 0.2 M - Sodium cacodylate 0.1 M pH 5.5 🔍	pH 5.5	100.0 μl of Ammonium sulfate (2 M) 500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium cacodylate pH 5.5 (1 M) 300.0 μl of water (100 v/v)
C5	PEG Smear Medium 25 v/v - tri-Sodium citrate 0.1 M pH 5.5 Rubidium chloride 0.1 M - Sodium Potassium phosphate 0.1 M pH 5.5 🔍	NA	500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Rubidium chloride (1 M) 100.0 μl of Sodium Potassium phosphate pH 5.5 (1 M) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 200.0 μl of water (100 v/v)
C6	PEG Smear High 22.5 v/v - Potassium chloride 0.2 M - 🔍	NA	450.0 μl of PEG Smear High (50 v/v) 200.0 μl of Potassium chloride (1 M) 350.0 μl of water (100 v/v)
C7	PEG Smear High 15 v/v - Ammonium acetate 0.15 M - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	150.0 μl of Ammonium acetate (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 450.0 μl of water (100 v/v)
C8	PEG Smear Broad 28 v/v - L-Arginine 0.05 M - L-Glutamic acid 0.05 M - Glycerol 5 v/v - 🔍	NA	166.7 μl of Glycerol (30 v/v) 50.0 μl of L-Arginine (1 M) 50.0 μl of L-Glutamic acid (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 173.3 μl of water (100 v/v)
C9	PEG Smear Broad 20 v/v - Magnesium acetate 0.15 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	150.0 μl of Magnesium acetate (1 M) 400.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 350.0 μl of water (100 v/v)
C10	PEG Smear Broad 25 v/v - Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	200.0 μl of Ammonium sulfate (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of water (100 v/v)
C11	PEG Smear Low 25 v/v - MES 0.1 M pH 6 Potassium Sodium tartrate 0.2 M - 🔍	pH 6	100.0 μl of MES pH 6 (1 M) 500.0 μl of PEG Smear Low (50 v/v) 200.0 μl of Potassium Sodium tartrate (1 M) 200.0 μl of water (100 v/v)
C12	PEG Smear Medium 22.5 v/v - Calcium chloride 0.1 M - Magnesium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	50.0 μl of Calcium chloride (2 M) 100.0 μl of Magnesium chloride (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 300.0 μl of water (100 v/v)
D1	PEG Smear Low 22.5 v/v - Ammonium nitrate 0.2 M - Sodium cacodylate 0.1 M pH 5.3 🔍	pH 5.3	100.0 μl of Ammonium nitrate (2 M) 450.0 μl of PEG Smear Low (50 v/v) 51.0 μl of Sodium cacodylate pH 5 (1 M) 49.0 μl of Sodium cacodylate pH 5.5 (1 M) 350.0 μl of water (100 v/v)
D2	PEG Smear Low 22.5 v/v - 2-Propanol 10 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	333.3 μl of 2-Propanol (30 v/v) 100.0 μl of MES pH 6.5 (1 M) 450.0 μl of PEG Smear Low (50 v/v) 116.7 μl of water (100 v/v)
D3	PEG Smear Medium 20 v/v - Ethylene glycol 5 v/v - MES 0.1 M pH 6 Ammonium nitrate 0.15 M - 🔍	pH 6	75.0 μl of Ammonium nitrate (2 M) 166.7 μl of Ethylene glycol (30 v/v) 100.0 μl of MES pH 6 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 258.3 μl of water (100 v/v)
D4	PEG Smear Medium 20 v/v - Glycerol 10 v/v - Sodium formate 0.2 M - Sodium phosphate citrate 0.1 M pH 6.2 🔍	pH 6.2	200.0 μl of Glycerol (50 v/v) 400.0 μl of PEG Smear Medium (50 v/v) 50.0 μl of Sodium formate (4 M) 100.0 μl of Sodium phosphate citrate pH 6.2 (1 M) 250.0 μl of water (100 v/v)
D5	PEG Smear Medium 30 v/v - Lithium sulfate 0.2 M - ADA 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 600.0 μl of PEG Smear Medium (50 v/v) 200.0 μl of water (100 v/v)
D6	PEG Smear High 12 v/v - Potassium thiocyanate 0.1 M - Sodium bromide 0.1 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 240.0 μl of PEG Smear High (50 v/v) 12.5 μl of Potassium thiocyanate (8 M) 20.0 μl of Sodium bromide (5 M) 627.5 μl of water (100 v/v)
D7	PEG Smear High 18 v/v - Ammonium sulfate 0.2 M - ADA 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Ammonium sulfate (4 M) 360.0 μl of PEG Smear High (50 v/v) 490.0 μl of water (100 v/v)
D8	PEG Smear Broad 15 v/v - Glycerol 5 v/v - Calcium chloride 0.15 M - MES 0.1 M pH 6.2 🔍	pH 6.2	75.0 μl of Calcium chloride (2 M) 166.7 μl of Glycerol (30 v/v) 100.0 μl of MES pH 6.2 (1 M) 300.0 μl of PEG Smear Broad (50 v/v) 358.3 μl of water (100 v/v)
D9	PEG Smear Broad 15 v/v - Ethylene glycol 10 w/v - Sodium cacodylate 0.1 M pH 5.3 T-Mate 5 v/v - 🔍	pH 5.3	200.0 μl of Ethylene glycol (50 w/v) 300.0 μl of PEG Smear Broad (50 v/v) 49.0 μl of Sodium cacodylate pH 5.5 (1 M) 51.0 μl of Sodium cacodylate pH 5 (1 M) 50.0 μl of T-Mate (100 v/v) 350.0 μl of water (100 v/v)
D10	PEG Smear Broad 28 v/v - Sodium chloride 0.2 M - Sodium phosphate citrate 0.1 M pH 6.2 🔍	pH 6.2	560.0 μl of PEG Smear Broad (50 v/v) 200.0 μl of Sodium chloride (1 M) 100.0 μl of Sodium phosphate citrate pH 6.2 (1 M) 140.0 μl of water (100 v/v)
D11	PEG Smear Medium 22.5 v/v - tri-Sodium citrate 0.1 M pH 5.5 Magnesium sulfate 0.05 M - Ammonium sulfate 0.1 M - 🔍	pH 5.5	100.0 μl of Ammonium sulfate (1 M) 50.0 μl of Magnesium sulfate (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 300.0 μl of water (100 v/v)
D12	PEG Smear Medium 22.5 v/v - Glycerol 2 v/v - Cobalt (II) chloride 0.01 M - Magnesium chloride 0.2 M - BIS-TRIS propane 0.1 M pH 8 🔍	pH 8	72.9 μl of BIS-TRIS propane pH 8.5 (1 M) 27.1 μl of BIS-TRIS propane pH 7.5 (1 M) 10.0 μl of Cobalt (II) chloride (1 M) 66.7 μl of Glycerol (30 v/v) 100.0 μl of Magnesium chloride (2 M) 450.0 μl of PEG Smear Medium (50 v/v) 273.3 μl of water (100 v/v)
E1	PEG Smear Low 25 v/v - Magnesium acetate 0.08 M - Magnesium chloride 0.02 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium acetate (1 M) 20.0 μl of Magnesium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v)
E2	PEG Smear Low 16 v/v - Ethylene glycol 5 v/v - Potassium chloride 0.1 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	166.7 μl of Ethylene glycol (30 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Potassium chloride (1 M) 313.3 μl of water (100 v/v)
E3	PEG Smear Medium 20 v/v - Zinc acetate 0.1 M - Zinc chloride 0.1 M - BIS-TRIS 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of BIS-TRIS pH 7.5 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Zinc acetate (1 M) 100.0 μl of Zinc chloride (1 M) 300.0 μl of water (100 v/v)
E4	PEG Smear Medium 20 v/v - Magnesium chloride 0.1 M - Potassium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	20.0 μl of Magnesium chloride (5 M) 400.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Potassium chloride (1 M) 380.0 μl of water (100 v/v)
E5	PEG Smear Medium 28 v/v - Magnesium sulfate 0.05 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Magnesium sulfate (1 M) 560.0 μl of PEG Smear Medium (50 v/v) 290.0 μl of water (100 v/v)
E6	PEG Smear High 15 v/v - Ethylene glycol 10 v/v - Sodium Potassium phosphate 0.1 M pH 7.5 HEPES 0.1 M pH 7.5 🔍	NA	333.3 μl of Ethylene glycol (30 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of Sodium Potassium phosphate pH 7.5 (1 M) 166.7 μl of water (100 v/v)
E7	PEG Smear High 25 v/v - Magnesium formate 0.1 M - Rubidium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	100.0 μl of Magnesium formate (1 M) 500.0 μl of PEG Smear High (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Rubidium chloride (1 M) 200.0 μl of water (100 v/v)
E8	PEG Smear Broad 25 v/v - Lithium sulfate 0.2 M - HEPES 0.1 M pH 7.2 🔍	pH 7.2	36.0 μl of HEPES pH 7.5 (1 M) 64.0 μl of HEPES pH 7 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of PEG Smear Broad (50 v/v) 300.0 μl of water (100 v/v)
E9	PEG Smear Broad 20 v/v - Ammonium nitrate 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of Ammonium nitrate (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of PEG Smear Broad (50 v/v) 400.0 μl of water (100 v/v)
E10	PEG Smear Broad 30 v/v - Magnesium chloride 0.1 M - Rubidium chloride 0.1 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Magnesium chloride (2 M) 600.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Rubidium chloride (1 M) 150.0 μl of water (100 v/v)
E11	PEG Smear High 22.5 v/v - Magnesium chloride 0.05 M - tri-Sodium citrate 0.05 M - BIS-TRIS propane 0.1 M pH 7.8 🔍	pH 7.8	51.4 μl of BIS-TRIS propane pH 8.5 (1 M) 48.6 μl of BIS-TRIS propane pH 7.5 (1 M) 50.0 μl of Magnesium chloride (1 M) 450.0 μl of PEG Smear High (50 v/v) 50.0 μl of tri-Sodium citrate (1 M) 350.0 μl of water (100 v/v)
E12	PEG Smear High 22.5 v/v - Ethylene glycol 10 v/v - BICINE 0.1 M pH 9 T-Mate 7 v/v - 🔍	pH 9	100.0 μl of BICINE pH 9 (1 M) 333.3 μl of Ethylene glycol (30 v/v) 450.0 μl of PEG Smear High (50 v/v) 70.0 μl of T-Mate (100 v/v) 46.7 μl of water (100 v/v)
F1	PEG Smear Low 25 v/v - tri-Sodium citrate 0.15 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100.0 μl of HEPES pH 7.8 (1 M) 500.0 μl of PEG Smear Low (50 v/v) 150.0 μl of tri-Sodium citrate (1 M) 250.0 μl of water (100 v/v)
F2	PEG Smear Low 28 v/v - Glycerol 5 v/v - Sodium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	166.7 μl of Glycerol (30 v/v) 560.0 μl of PEG Smear Low (50 v/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 133.3 μl of water (100 v/v)
F3	PEG Smear Medium 15 v/v - Sodium acetate 0.08 M - Sodium chloride 0.15 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	300.0 μl of PEG Smear Medium (50 v/v) 40.0 μl of Sodium acetate (2 M) 75.0 μl of Sodium chloride (2 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 485.0 μl of water (100 v/v)
F4	PEG Smear Medium 25 v/v - Sodium chloride 0.1 M - Sodium formate 0.1 M - BIS-TRIS 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of BIS-TRIS pH 8.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 50.0 μl of Sodium chloride (2 M) 25.0 μl of Sodium formate (4 M) 325.0 μl of water (100 v/v)
F5	PEG Smear Medium 20 v/v - Ammonium sulfate 0.2 M - Magnesium sulfate 0.05 M - BICINE 0.1 M pH 9 🔍	pH 9	200.0 μl of Ammonium sulfate (1 M) 100.0 μl of BICINE pH 9 (1 M) 50.0 μl of Magnesium sulfate (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 250.0 μl of water (100 v/v)
F6	PEG Smear High 18 v/v - Ammonium nitrate 0.2 M - BIS-TRIS propane 0.1 M pH 8.5 🔍	pH 8.5	100.0 μl of Ammonium nitrate (2 M) 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 360.0 μl of PEG Smear High (50 v/v) 440.0 μl of water (100 v/v)
F7	PEG Smear High 25 v/v - Glycerol 10 v/v - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100.0 μl of Glycerol (100 v/v) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of PEG Smear High (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 200.0 μl of water (100 v/v)
F8	PEG Smear Broad 28 v/v - Ammonium acetate 0.15 M - Calcium chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	150.0 μl of Ammonium acetate (1 M) 10.0 μl of Calcium chloride (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 180.0 μl of water (100 v/v)
F9	PEG Smear Broad 25 v/v - 2-Propanol 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 300.0 μl of water (100 v/v)
F10	PEG Smear Broad 20 v/v - Ammonium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100.0 μl of Ammonium sulfate (2 M) 400.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 400.0 μl of water (100 v/v)
F11	PEG Smear Broad 22.5 v/v - Magnesium sulfate 0.02 M - Potassium chloride 0.2 M - BICINE 0.1 M pH 8.8 🔍	pH 8.8	100.0 μl of BICINE pH 8.8 (1 M) 20.0 μl of Magnesium sulfate (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 200.0 μl of Potassium chloride (1 M) 230.0 μl of water (100 v/v)
F12	PEG Smear Broad 22.5 v/v - Ethylene glycol 10 v/v - Potassium Sodium tartrate 0.1 M - Sodium cacodylate 0.1 M pH 5.5 🔍	pH 5.5	100.0 μl of Ethylene glycol (100 v/v) 450.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Potassium Sodium tartrate (1 M) 100.0 μl of Sodium cacodylate pH 5.5 (1 M) 250.0 μl of water (100 v/v)
G1	PEG Smear Low 14 v/v - Cobalt (II) chloride 0.01 M - Magnesium formate 0.1 M - MES 0.1 M pH 6.2 🔍	pH 6.2	10.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.2 (1 M) 100.0 μl of Magnesium formate (1 M) 280.0 μl of PEG Smear Low (50 v/v) 510.0 μl of water (100 v/v)
G2	PEG Smear Low 25 v/v - Lithium sulfate 0.15 M - Magnesium chloride 0.05 M - BIS-TRIS 0.1 M pH 6.8 🔍	pH 6.8	100.0 μl of BIS-TRIS pH 6.8 (1 M) 150.0 μl of Lithium sulfate (1 M) 50.0 μl of Magnesium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 200.0 μl of water (100 v/v)
G3	PEG Smear Medium 25 v/v - Ammonium sulfate 0.2 M - Cadmium chloride 0.01 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200.0 μl of Ammonium sulfate (1 M) 10.0 μl of Cadmium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 190.0 μl of water (100 v/v)
G4	PEG Smear Medium 18 v/v - Ethylene glycol 10 v/v - Potassium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	NA	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium chloride (1 M) 360.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Potassium chloride (1 M) 340.0 μl of water (100 v/v)
G5	PEG Smear Medium 12 v/v - Ethylene glycol 10 v/v - Magnesium acetate 0.1 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (1 M) 240.0 μl of PEG Smear Medium (50 v/v) 460.0 μl of water (100 v/v)
G6	PEG Smear High 12 v/v - Magnesium acetate 0.1 M - Potassium chloride 0.1 M - MES 0.1 M pH 6.2 🔍	pH 6.2	100.0 μl of MES pH 6.2 (1 M) 100.0 μl of Magnesium acetate (1 M) 240.0 μl of PEG Smear High (50 v/v) 100.0 μl of Potassium chloride (1 M) 460.0 μl of water (100 v/v)
G7	PEG Smear High 8 v/v - Calcium chloride 0.04 M - Sodium formate 0.04 M - PIPES 0.1 M pH 7 🔍	pH 7	400.0 μl of Calcium chloride (0.1 M) 160.0 μl of PEG Smear High (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 10.0 μl of Sodium formate (4 M) 330.0 μl of water (100 v/v)
G8	PEG Smear Broad 18 v/v - Magnesium chloride 0.08 M - tri-Sodium citrate 0.08 M - BIS-TRIS 0.1 M pH 6 🔍	pH 6	100.0 μl of BIS-TRIS pH 6 (1 M) 80.0 μl of Magnesium chloride (1 M) 360.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of tri-Sodium citrate (1.6 M) 410.0 μl of water (100 v/v)
G9	PEG Smear Broad 15 v/v - Magnesium chloride 0.1 M - Sodium acetate 0.1 M - BIS-TRIS 0.1 M pH 6.5 🔍	pH 6.5	100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Magnesium chloride (1 M) 300.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of Sodium acetate (2 M) 450.0 μl of water (100 v/v)
G10	PEG Smear Broad 25 v/v - Ammonium sulfate 0.1 M - Sodium formate 0.1 M - HEPES 0.1 M pH 7 🔍	pH 7	100.0 μl of Ammonium sulfate (1 M) 100.0 μl of HEPES pH 7 (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 25.0 μl of Sodium formate (4 M) 275.0 μl of water (100 v/v)
G11	PEG Smear Medium 22.5 v/v - Glycerol 10 v/v - Sodium Potassium phosphate 0.2 M pH 7.5 HEPES 0.1 M pH 7.5 🔍	NA	100.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium Potassium phosphate pH 7.5 (2 M) 250.0 μl of water (100 v/v)
G12	PEG Smear Broad 22.5 v/v - L-Glutamic acid 0.05 M - Sodium chloride 0.3 M - L-Arginine 0.05 M - tris chloride BCC 0.1 M pH 7.5 🔍	pH 7.5	50.0 μl of L-Arginine (1 M) 50.0 μl of L-Glutamic acid (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 150.0 μl of Sodium chloride (2 M) 100.0 μl of tris chloride BCC pH 7.5 (1 M) 200.0 μl of water (100 v/v)
H1	PEG Smear Low 25 v/v - Calcium chloride 0.04 M - Sodium formate 0.04 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	40.0 μl of Calcium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 10.0 μl of Sodium formate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 350.0 μl of water (100 v/v)
H2	PEG Smear Low 20 v/v - Magnesium chloride 0.1 M - Rubidium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	100.0 μl of Magnesium chloride (1 M) 400.0 μl of PEG Smear Low (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Rubidium chloride (1 M) 300.0 μl of water (100 v/v)
H3	PEG Smear Medium 15 v/v - 2-Propanol 5 v/v - Magnesium chloride 0.2 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	50.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ethylene glycol (50 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of PEG Smear Medium (50 v/v) 250.0 μl of water (100 v/v)
H4	PEG Smear Medium 12 v/v - Ammonium acetate 0.05 M - Magnesium sulfate 0.15 M - HEPES 0.1 M pH 7 🔍	pH 7	50.0 μl of Ammonium acetate (1 M) 100.0 μl of HEPES pH 7 (1 M) 150.0 μl of Magnesium sulfate (1 M) 240.0 μl of PEG Smear Medium (50 v/v) 460.0 μl of water (100 v/v)
H5	PEG Smear Medium 20 v/v - HEPES 0.1 M pH 7.2 T-Mate 7 v/v - 🔍	pH 7.2	51.9 μl of HEPES pH 7.1 (1 M) 48.1 μl of HEPES pH 7.3 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 70.0 μl of T-Mate (100 v/v) 430.0 μl of water (100 v/v)
H6	PEG Smear High 15 v/v - Ammonium acetate 0.1 M - Zinc chloride 0.1 M - BIS-TRIS 0.1 M pH 7.2 🔍	pH 7.2	100.0 μl of Ammonium acetate (1 M) 50.5 μl of BIS-TRIS pH 7 (1 M) 49.5 μl of BIS-TRIS pH 7.5 (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of Zinc chloride (1 M) 400.0 μl of water (100 v/v)
H7	PEG Smear High 20 v/v - Lithium sulfate 0.15 M - Magnesium chloride 0.05 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100.0 μl of HEPES pH 7.8 (1 M) 150.0 μl of Lithium sulfate (1 M) 50.0 μl of Magnesium chloride (1 M) 400.0 μl of PEG Smear High (50 v/v) 300.0 μl of water (100 v/v)
H8	PEG Smear Broad 25 v/v - Potassium thiocyanate 100 mM - Sodium bromide 0.1 M - tris chloride BCC 0.1 M pH 7.8 🔍	pH 7.8	500.0 μl of PEG Smear Broad (50 v/v) 200.0 μl of Potassium thiocyanate (500 mM) 20.0 μl of Sodium bromide (5 M) 74.1 μl of tris chloride BCC pH 7.5 (1 M) 25.9 μl of tris chloride BCC pH 8.5 (1 M) 180.0 μl of water (100 v/v)
H9	PEG Smear Broad 28 v/v - Ammonium sulfate 0.05 M - Lithium sulfate 0.05 M - BIS-TRIS propane 0.1 M pH 8.5 🔍	pH 8.5	50.0 μl of Ammonium sulfate (1 M) 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 50.0 μl of Lithium sulfate (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 240.0 μl of water (100 v/v)
H10	PEG Smear Broad 15 v/v - Ethylene glycol 10 v/v - Ammonium sulfate 0.2 M - Cadmium chloride 0.01 M - PIPES 0.1 M pH 7 🔍	pH 7	200.0 μl of Ammonium sulfate (1 M) 100.0 μl of Cadmium chloride (0.1 M) 100.0 μl of Ethylene glycol (100 v/v) 300.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 200.0 μl of water (100 v/v)
H11	PEG Smear Broad 22.5 v/v - Lithium sulfate 0.2 M - Zinc acetate 0.05 M - BIS-TRIS 0.1 M pH 7.5 🔍	pH 7.5	100.0 μl of BIS-TRIS pH 7.5 (1 M) 200.0 μl of Lithium sulfate (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of Zinc acetate (1 M) 200.0 μl of water (100 v/v)
H12	PEG Smear Broad 22.5 v/v - Sodium iodide 80 mM - Sodium bromide 0.08 M - Sodium fluoride 0.05 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100.0 μl of HEPES pH 7.8 (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 16.0 μl of Sodium bromide (5 M) 25.0 μl of Sodium fluoride (2 M) 160.0 μl of Sodium iodide (500 mM) 249.0 μl of water (100 v/v)