C6 - Screen Contents

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Supplemental Information

Screen Description

BCS

Molecular Dimensions / BCS

Created 2018-08-03.

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BCS

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BCS

Screen Structure

BCS

Well Count	96
Number of distinct ingredients	47
Internal diversity (score mean dist)	0.929

Screen Composition

More information about screen composition chart.

ingredient type	BCS
Buffer	117
Complex ingredient	96
Cryoprotectant	7
Organic (non-volatile)	18
Organic (volatile)	3
Salt	124

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

BCS Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Sodium acetate 0.1 M pH 4.5 PEG Smear Low 30 v/v - 🔍	pH 4.5	100 μl of Sodium acetate pH 4.5 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
A2	Sodium phosphate citrate 0.1 M pH 5.5 PEG Smear Low 30 v/v - 🔍	pH 5.5	0 μl of Sodium phosphate citrate pH 5 (1 M) 99 μl of Sodium phosphate citrate pH 6 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
A3	MES 0.1 M pH 6.5 PEG Smear Low 30 v/v - 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
A4	PEG Smear Medium 25 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	100 μl of Sodium acetate pH 4.5 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
A5	PEG Smear Medium 25 v/v - Sodium phosphate citrate 0.1 M pH 5.5 🔍	pH 5.5	0 μl of Sodium phosphate citrate pH 5 (1 M) 99 μl of Sodium phosphate citrate pH 6 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
A6	PEG Smear Medium 25 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
A7	Sodium acetate 0.1 M pH 4.5 PEG Smear High 20 v/v - 🔍	pH 4.5	400 μl of PEG Smear High (50 v/v) 100 μl of Sodium acetate pH 4.5 (1 M) 500 μl of water (100 v/v)
A8	Sodium phosphate citrate 0.1 M pH 5.5 PEG Smear High 20 v/v - 🔍	pH 5.5	0 μl of Sodium phosphate citrate pH 5 (1 M) 400 μl of PEG Smear High (50 v/v) 99 μl of Sodium phosphate citrate pH 6 (1 M) 500 μl of water (100 v/v)
A9	MES 0.1 M pH 6.5 PEG Smear High 20 v/v - 🔍	pH 6.5	400 μl of PEG Smear High (50 v/v) 100 μl of MES pH 6.5 (1 M) 500 μl of water (100 v/v)
A10	PEG Smear Broad 22 v/v - Sodium acetate 0.1 M pH 4.5 🔍	pH 4.5	100 μl of Sodium acetate pH 4.5 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
A11	PEG Smear Broad 22 v/v - Sodium phosphate citrate 0.1 M pH 5.5 🔍	pH 5.5	0 μl of Sodium phosphate citrate pH 5 (1 M) 99 μl of Sodium phosphate citrate pH 6 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
A12	PEG Smear Broad 22 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
B1	PEG Smear Low 30 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
B2	PEG Smear Low 30 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
B3	PEG Smear Low 30 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100 μl of BICINE pH 9.3 (1 M) 600 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
B4	PEG Smear Medium 25 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
B5	PEG Smear Medium 25 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
B6	PEG Smear Medium 25 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100 μl of BICINE pH 9.3 (1 M) 500 μl of PEG Smear Medium (50 v/v) 400 μl of water (100 v/v)
B7	PEG Smear High 20 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	400 μl of PEG Smear High (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 500 μl of water (100 v/v)
B8	PEG Smear High 20 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 400 μl of PEG Smear High (50 v/v) 500 μl of water (100 v/v)
B9	PEG Smear High 20 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100 μl of BICINE pH 9.3 (1 M) 400 μl of PEG Smear High (50 v/v) 500 μl of water (100 v/v)
B10	PEG Smear Broad 22 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
B11	PEG Smear Broad 22 v/v - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	100 μl of tris chloride BCC pH 8.5 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
B12	PEG Smear Broad 22 v/v - BICINE 0.1 M pH 9.3 🔍	pH 9.3	100 μl of BICINE pH 9.3 (1 M) 440 μl of PEG Smear Broad (50 v/v) 460 μl of water (100 v/v)
C1	PEG Smear Low 35 v/v - 🔍	NA	700 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
C2	PEG Smear Low 28 v/v - Ethylene glycol 5 v/v - Sodium acetate 0.1 M pH 4.6 Ammonium acetate 0.2 M - 🔍	pH 4.6	100 μl of Sodium acetate pH 4.6 (1 M) 166 μl of Ethylene glycol (30 v/v) 66 μl of Ammonium acetate (3 M) 560 μl of PEG Smear Low (50 v/v) 106 μl of water (100 v/v)
C3	PEG Smear Medium 28 v/v - Sodium chloride 0.15 M - 🔍	NA	75 μl of Sodium chloride (2 M) 560 μl of PEG Smear Medium (50 v/v) 365 μl of water (100 v/v)
C4	PEG Smear Medium 25 v/v - Ammonium sulfate 0.2 M - Sodium cacodylate 0.1 M pH 5.5 🔍	pH 5.5	100 μl of Sodium cacodylate pH 5.5 (1 M) 100 μl of Ammonium sulfate (2 M) 500 μl of PEG Smear Medium (50 v/v) 300 μl of water (100 v/v)
C5	PEG Smear Medium 25 v/v - tri-Sodium citrate 0.1 M pH 5.5 Rubidium chloride 0.1 M - Sodium Potassium phosphate 0.1 M pH 5.5 🔍	NA	100 μl of tri-Sodium citrate pH 5.5 (1 M) 100 μl of Rubidium chloride (1 M) 500 μl of PEG Smear Medium (50 v/v) 100 μl of Sodium Potassium phosphate pH 5.5 (1 M) 200 μl of water (100 v/v)
C6	PEG Smear High 22.5 v/v - Potassium chloride 0.2 M - 🔍	NA	450 μl of PEG Smear High (50 v/v) 200 μl of Potassium chloride (1 M) 350 μl of water (100 v/v)
C7	PEG Smear High 15 v/v - Ammonium acetate 0.15 M - tri-Sodium citrate 0.1 M pH 5 🔍	pH 5	150 μl of Ammonium acetate (1 M) 300 μl of PEG Smear High (50 v/v) 100 μl of tri-Sodium citrate pH 5 (1 M) 450 μl of water (100 v/v)
C8	PEG Smear Broad 28 v/v - L-Arginine 0.05 M - L-Glutamic acid 0.05 M - Glycerol 5 v/v - 🔍	NA	166 μl of Glycerol (30 v/v) 50 μl of L-Glutamic acid (1 M) 50 μl of L-Arginine (1 M) 560 μl of PEG Smear Broad (50 v/v) 173 μl of water (100 v/v)
C9	PEG Smear Broad 20 v/v - Magnesium acetate 0.15 M - tri-Sodium citrate 0.1 M pH 5.6 🔍	pH 5.6	100 μl of tri-Sodium citrate pH 5.6 (1 M) 150 μl of Magnesium acetate (1 M) 400 μl of PEG Smear Broad (50 v/v) 350 μl of water (100 v/v)
C10	PEG Smear Broad 25 v/v - Ammonium sulfate 0.2 M - Sodium acetate 0.1 M pH 4.6 🔍	pH 4.6	200 μl of Ammonium sulfate (1 M) 100 μl of Sodium acetate pH 4.6 (1 M) 500 μl of PEG Smear Broad (50 v/v) 200 μl of water (100 v/v)
C11	PEG Smear Low 25 v/v - MES 0.1 M pH 6 Potassium Sodium tartrate 0.2 M - 🔍	pH 6	100 μl of MES pH 6 (1 M) 500 μl of PEG Smear Low (50 v/v) 200 μl of Potassium Sodium tartrate (1 M) 200 μl of water (100 v/v)
C12	PEG Smear Medium 22.5 v/v - Calcium chloride 0.1 M - Magnesium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	50 μl of Calcium chloride (2 M) 100 μl of PIPES pH 7 (1 M) 100 μl of Magnesium chloride (1 M) 450 μl of PEG Smear Medium (50 v/v) 300 μl of water (100 v/v)
D1	PEG Smear Low 22.5 v/v - Ammonium nitrate 0.2 M - Sodium cacodylate 0.1 M pH 5.3 🔍	pH 5.3	49 μl of Sodium cacodylate pH 5.5 (1 M) 51 μl of Sodium cacodylate pH 5 (1 M) 100 μl of Ammonium nitrate (2 M) 450 μl of PEG Smear Low (50 v/v) 350 μl of water (100 v/v)
D2	PEG Smear Low 22.5 v/v - 2-Propanol 10 v/v - MES 0.1 M pH 6.5 🔍	pH 6.5	333 μl of 2-Propanol (30 v/v) 100 μl of MES pH 6.5 (1 M) 450 μl of PEG Smear Low (50 v/v) 116 μl of water (100 v/v)
D3	PEG Smear Medium 20 v/v - Ethylene glycol 5 v/v - MES 0.1 M pH 6 Ammonium nitrate 0.15 M - 🔍	pH 6	100 μl of MES pH 6 (1 M) 75 μl of Ammonium nitrate (2 M) 166 μl of Ethylene glycol (30 v/v) 400 μl of PEG Smear Medium (50 v/v) 258 μl of water (100 v/v)
D4	PEG Smear Medium 20 v/v - Glycerol 10 v/v - Sodium formate 0.2 M - Sodium phosphate citrate 0.1 M pH 6.2 🔍	pH 6.2	200 μl of Glycerol (50 v/v) 100 μl of Sodium phosphate citrate pH 6.2 (1 M) 50 μl of Sodium formate (4 M) 400 μl of PEG Smear Medium (50 v/v) 250 μl of water (100 v/v)
D5	PEG Smear Medium 30 v/v - Lithium sulfate 0.2 M - ADA 0.1 M pH 6.5 🔍	pH 6.5	100 μl of ADA pH 6.5 (1 M) 100 μl of Lithium sulfate (2 M) 600 μl of PEG Smear Medium (50 v/v) 200 μl of water (100 v/v)
D6	PEG Smear High 12 v/v - Potassium thiocyanate 0.1 M - Sodium bromide 0.1 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 20 μl of Sodium bromide (5 M) 12 μl of Potassium thiocyanate (8 M) 240 μl of PEG Smear High (50 v/v) 627 μl of water (100 v/v)
D7	PEG Smear High 18 v/v - Ammonium sulfate 0.2 M - ADA 0.1 M pH 6.5 🔍	pH 6.5	50 μl of Ammonium sulfate (4 M) 360 μl of PEG Smear High (50 v/v) 100 μl of ADA pH 6.5 (1 M) 490 μl of water (100 v/v)
D8	PEG Smear Broad 15 v/v - Glycerol 5 v/v - Calcium chloride 0.15 M - MES 0.1 M pH 6.2 🔍	pH 6.2	166 μl of Glycerol (30 v/v) 75 μl of Calcium chloride (2 M) 100 μl of MES pH 6.2 (1 M) 300 μl of PEG Smear Broad (50 v/v) 358 μl of water (100 v/v)
D9	PEG Smear Broad 15 v/v - Ethylene glycol 10 w/v - Sodium cacodylate 0.1 M pH 5.3 T-Mate 5 v/v - 🔍	pH 5.3	49 μl of Sodium cacodylate pH 5.5 (1 M) 51 μl of Sodium cacodylate pH 5 (1 M) 200 μl of Ethylene glycol (50 w/v) 300 μl of PEG Smear Broad (50 v/v) 50 μl of T-Mate (100 v/v) 350 μl of water (100 v/v)
D10	PEG Smear Broad 28 v/v - Sodium chloride 0.2 M - Sodium phosphate citrate 0.1 M pH 6.2 🔍	pH 6.2	100 μl of Sodium phosphate citrate pH 6.2 (1 M) 200 μl of Sodium chloride (1 M) 560 μl of PEG Smear Broad (50 v/v) 140 μl of water (100 v/v)
D11	PEG Smear Medium 22.5 v/v - tri-Sodium citrate 0.1 M pH 5.5 Magnesium sulfate 0.05 M - Ammonium sulfate 0.1 M - 🔍	pH 5.5	100 μl of Ammonium sulfate (1 M) 100 μl of tri-Sodium citrate pH 5.5 (1 M) 50 μl of Magnesium sulfate (1 M) 450 μl of PEG Smear Medium (50 v/v) 300 μl of water (100 v/v)
D12	PEG Smear Medium 22.5 v/v - Glycerol 2 v/v - Cobalt (II) chloride 0.01 M - Magnesium chloride 0.2 M - BIS-TRIS propane 0.1 M pH 8 🔍	pH 8	66 μl of Glycerol (30 v/v) 27 μl of BIS-TRIS propane pH 7.5 (1 M) 100 μl of Magnesium chloride (2 M) 72 μl of BIS-TRIS propane pH 8.5 (1 M) 450 μl of PEG Smear Medium (50 v/v) 10 μl of Cobalt (II) chloride (1 M) 273 μl of water (100 v/v)
E1	PEG Smear Low 25 v/v - Magnesium acetate 0.08 M - Magnesium chloride 0.02 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 20 μl of Magnesium chloride (1 M) 500 μl of PEG Smear Low (50 v/v) 80 μl of Magnesium acetate (1 M) 300 μl of water (100 v/v)
E2	PEG Smear Low 16 v/v - Ethylene glycol 5 v/v - Potassium chloride 0.1 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	166 μl of Ethylene glycol (30 v/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Potassium chloride (1 M) 320 μl of PEG Smear Low (50 v/v) 313 μl of water (100 v/v)
E3	PEG Smear Medium 20 v/v - Zinc acetate 0.1 M - Zinc chloride 0.1 M - BIS-TRIS 0.1 M pH 7.5 🔍	pH 7.5	100 μl of Zinc acetate (1 M) 100 μl of BIS-TRIS pH 7.5 (1 M) 400 μl of PEG Smear Medium (50 v/v) 100 μl of Zinc chloride (1 M) 300 μl of water (100 v/v)
E4	PEG Smear Medium 20 v/v - Magnesium chloride 0.1 M - Potassium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	100 μl of Potassium chloride (1 M) 400 μl of PEG Smear Medium (50 v/v) 100 μl of PIPES pH 7 (1 M) 20 μl of Magnesium chloride (5 M) 380 μl of water (100 v/v)
E5	PEG Smear Medium 28 v/v - Magnesium sulfate 0.05 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	50 μl of Magnesium sulfate (1 M) 560 μl of PEG Smear Medium (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 290 μl of water (100 v/v)
E6	PEG Smear High 15 v/v - Ethylene glycol 10 v/v - Sodium Potassium phosphate 0.1 M pH 7.5 HEPES 0.1 M pH 7.5 🔍	NA	300 μl of PEG Smear High (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Sodium Potassium phosphate pH 7.5 (1 M) 333 μl of Ethylene glycol (30 v/v) 166 μl of water (100 v/v)
E7	PEG Smear High 25 v/v - Magnesium formate 0.1 M - Rubidium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	100 μl of Magnesium formate (1 M) 500 μl of PEG Smear High (50 v/v) 100 μl of Rubidium chloride (1 M) 100 μl of PIPES pH 7 (1 M) 200 μl of water (100 v/v)
E8	PEG Smear Broad 25 v/v - Lithium sulfate 0.2 M - HEPES 0.1 M pH 7.2 🔍	pH 7.2	36 μl of HEPES pH 7.5 (1 M) 100 μl of Lithium sulfate (2 M) 500 μl of PEG Smear Broad (50 v/v) 64 μl of HEPES pH 7 (1 M) 300 μl of water (100 v/v)
E9	PEG Smear Broad 20 v/v - Ammonium nitrate 0.2 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of HEPES pH 7.5 (1 M) 100 μl of Ammonium nitrate (2 M) 400 μl of PEG Smear Broad (50 v/v) 400 μl of water (100 v/v)
E10	PEG Smear Broad 30 v/v - Magnesium chloride 0.1 M - Rubidium chloride 0.1 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	100 μl of Rubidium chloride (1 M) 50 μl of Magnesium chloride (2 M) 100 μl of HEPES pH 7.5 (1 M) 600 μl of PEG Smear Broad (50 v/v) 150 μl of water (100 v/v)
E11	PEG Smear High 22.5 v/v - Magnesium chloride 0.05 M - tri-Sodium citrate 0.05 M - BIS-TRIS propane 0.1 M pH 7.8 🔍	pH 7.8	450 μl of PEG Smear High (50 v/v) 48 μl of BIS-TRIS propane pH 7.5 (1 M) 51 μl of BIS-TRIS propane pH 8.5 (1 M) 50 μl of Magnesium chloride (1 M) 50 μl of tri-Sodium citrate (1 M) 350 μl of water (100 v/v)
E12	PEG Smear High 22.5 v/v - Ethylene glycol 10 v/v - BICINE 0.1 M pH 9 T-Mate 7 v/v - 🔍	pH 9	450 μl of PEG Smear High (50 v/v) 333 μl of Ethylene glycol (30 v/v) 100 μl of BICINE pH 9 (1 M) 70 μl of T-Mate (100 v/v) 46 μl of water (100 v/v)
F1	PEG Smear Low 25 v/v - tri-Sodium citrate 0.15 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100 μl of HEPES pH 7.8 (1 M) 150 μl of tri-Sodium citrate (1 M) 500 μl of PEG Smear Low (50 v/v) 250 μl of water (100 v/v)
F2	PEG Smear Low 28 v/v - Glycerol 5 v/v - Sodium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	166 μl of Glycerol (30 v/v) 40 μl of Sodium chloride (5 M) 560 μl of PEG Smear Low (50 v/v) 100 μl of tris chloride BCC pH 8.5 (1 M) 133 μl of water (100 v/v)
F3	PEG Smear Medium 15 v/v - Sodium acetate 0.08 M - Sodium chloride 0.15 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	75 μl of Sodium chloride (2 M) 40 μl of Sodium acetate (2 M) 100 μl of tris chloride BCC pH 8 (1 M) 300 μl of PEG Smear Medium (50 v/v) 485 μl of water (100 v/v)
F4	PEG Smear Medium 25 v/v - Sodium chloride 0.1 M - Sodium formate 0.1 M - BIS-TRIS 0.1 M pH 8.5 🔍	pH 8.5	50 μl of Sodium chloride (2 M) 25 μl of Sodium formate (4 M) 100 μl of BIS-TRIS pH 8.5 (1 M) 500 μl of PEG Smear Medium (50 v/v) 325 μl of water (100 v/v)
F5	PEG Smear Medium 20 v/v - Ammonium sulfate 0.2 M - Magnesium sulfate 0.05 M - BICINE 0.1 M pH 9 🔍	pH 9	200 μl of Ammonium sulfate (1 M) 100 μl of BICINE pH 9 (1 M) 50 μl of Magnesium sulfate (1 M) 400 μl of PEG Smear Medium (50 v/v) 250 μl of water (100 v/v)
F6	PEG Smear High 18 v/v - Ammonium nitrate 0.2 M - BIS-TRIS propane 0.1 M pH 8.5 🔍	pH 8.5	100 μl of Ammonium nitrate (2 M) 360 μl of PEG Smear High (50 v/v) 100 μl of BIS-TRIS propane pH 8.5 (1 M) 440 μl of water (100 v/v)
F7	PEG Smear High 25 v/v - Glycerol 10 v/v - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	500 μl of PEG Smear High (50 v/v) 100 μl of Glycerol (100 v/v) 100 μl of tris chloride BCC pH 8 (1 M) 100 μl of Magnesium chloride (2 M) 200 μl of water (100 v/v)
F8	PEG Smear Broad 28 v/v - Ammonium acetate 0.15 M - Calcium chloride 0.01 M - tris chloride BCC 0.1 M pH 8.5 🔍	pH 8.5	150 μl of Ammonium acetate (1 M) 100 μl of tris chloride BCC pH 8.5 (1 M) 10 μl of Calcium chloride (1 M) 560 μl of PEG Smear Broad (50 v/v) 180 μl of water (100 v/v)
F9	PEG Smear Broad 25 v/v - 2-Propanol 10 v/v - BICINE 0.1 M pH 9 🔍	pH 9	100 μl of 2-Propanol (100 v/v) 100 μl of BICINE pH 9 (1 M) 500 μl of PEG Smear Broad (50 v/v) 300 μl of water (100 v/v)
F10	PEG Smear Broad 20 v/v - Ammonium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 100 μl of Ammonium sulfate (2 M) 400 μl of PEG Smear Broad (50 v/v) 400 μl of water (100 v/v)
F11	PEG Smear Broad 22.5 v/v - Magnesium sulfate 0.02 M - Potassium chloride 0.2 M - BICINE 0.1 M pH 8.8 🔍	pH 8.8	100 μl of BICINE pH 8.8 (1 M) 200 μl of Potassium chloride (1 M) 450 μl of PEG Smear Broad (50 v/v) 20 μl of Magnesium sulfate (1 M) 230 μl of water (100 v/v)
F12	PEG Smear Broad 22.5 v/v - Ethylene glycol 10 v/v - Potassium Sodium tartrate 0.1 M - Sodium cacodylate 0.1 M pH 5.5 🔍	pH 5.5	100 μl of Ethylene glycol (100 v/v) 100 μl of Sodium cacodylate pH 5.5 (1 M) 450 μl of PEG Smear Broad (50 v/v) 100 μl of Potassium Sodium tartrate (1 M) 250 μl of water (100 v/v)
G1	PEG Smear Low 14 v/v - Cobalt (II) chloride 0.01 M - Magnesium formate 0.1 M - MES 0.1 M pH 6.2 🔍	pH 6.2	100 μl of MES pH 6.2 (1 M) 100 μl of Magnesium formate (1 M) 280 μl of PEG Smear Low (50 v/v) 10 μl of Cobalt (II) chloride (1 M) 510 μl of water (100 v/v)
G2	PEG Smear Low 25 v/v - Lithium sulfate 0.15 M - Magnesium chloride 0.05 M - BIS-TRIS 0.1 M pH 6.8 🔍	pH 6.8	150 μl of Lithium sulfate (1 M) 50 μl of Magnesium chloride (1 M) 500 μl of PEG Smear Low (50 v/v) 100 μl of BIS-TRIS pH 6.8 (1 M) 200 μl of water (100 v/v)
G3	PEG Smear Medium 25 v/v - Ammonium sulfate 0.2 M - Cadmium chloride 0.01 M - HEPES 0.1 M pH 7.5 🔍	pH 7.5	200 μl of Ammonium sulfate (1 M) 100 μl of HEPES pH 7.5 (1 M) 10 μl of Cadmium chloride (1 M) 500 μl of PEG Smear Medium (50 v/v) 190 μl of water (100 v/v)
G4	PEG Smear Medium 18 v/v - Ethylene glycol 10 v/v - Potassium chloride 0.1 M - Magnesium chloride 0.1 M - 🔍	NA	100 μl of Ethylene glycol (100 v/v) 100 μl of Magnesium chloride (1 M) 100 μl of Potassium chloride (1 M) 360 μl of PEG Smear Medium (50 v/v) 340 μl of water (100 v/v)
G5	PEG Smear Medium 12 v/v - Ethylene glycol 10 v/v - Magnesium acetate 0.1 M - MES 0.1 M pH 6.5 🔍	pH 6.5	100 μl of MES pH 6.5 (1 M) 100 μl of Ethylene glycol (100 v/v) 240 μl of PEG Smear Medium (50 v/v) 100 μl of Magnesium acetate (1 M) 460 μl of water (100 v/v)
G6	PEG Smear High 12 v/v - Magnesium acetate 0.1 M - Potassium chloride 0.1 M - MES 0.1 M pH 6.2 🔍	pH 6.2	240 μl of PEG Smear High (50 v/v) 100 μl of MES pH 6.2 (1 M) 100 μl of Potassium chloride (1 M) 100 μl of Magnesium acetate (1 M) 460 μl of water (100 v/v)
G7	PEG Smear High 8 v/v - Calcium chloride 0.04 M - Sodium formate 0.04 M - PIPES 0.1 M pH 7 🔍	pH 7	160 μl of PEG Smear High (50 v/v) 100 μl of PIPES pH 7 (1 M) 400 μl of Calcium chloride (0.1 M) 10 μl of Sodium formate (4 M) 330 μl of water (100 v/v)
G8	PEG Smear Broad 18 v/v - Magnesium chloride 0.08 M - tri-Sodium citrate 0.08 M - BIS-TRIS 0.1 M pH 6 🔍	pH 6	80 μl of Magnesium chloride (1 M) 360 μl of PEG Smear Broad (50 v/v) 100 μl of BIS-TRIS pH 6 (1 M) 50 μl of tri-Sodium citrate (1.6 M) 410 μl of water (100 v/v)
G9	PEG Smear Broad 15 v/v - Magnesium chloride 0.1 M - Sodium acetate 0.1 M - BIS-TRIS 0.1 M pH 6.5 🔍	pH 6.5	50 μl of Sodium acetate (2 M) 100 μl of Magnesium chloride (1 M) 300 μl of PEG Smear Broad (50 v/v) 100 μl of BIS-TRIS pH 6.5 (1 M) 450 μl of water (100 v/v)
G10	PEG Smear Broad 25 v/v - Ammonium sulfate 0.1 M - Sodium formate 0.1 M - HEPES 0.1 M pH 7 🔍	pH 7	100 μl of Ammonium sulfate (1 M) 100 μl of HEPES pH 7 (1 M) 25 μl of Sodium formate (4 M) 500 μl of PEG Smear Broad (50 v/v) 275 μl of water (100 v/v)
G11	PEG Smear Medium 22.5 v/v - Glycerol 10 v/v - Sodium Potassium phosphate 0.2 M pH 7.5 HEPES 0.1 M pH 7.5 🔍	NA	100 μl of Sodium Potassium phosphate pH 7.5 (2 M) 100 μl of HEPES pH 7.5 (1 M) 100 μl of Glycerol (100 v/v) 450 μl of PEG Smear Medium (50 v/v) 250 μl of water (100 v/v)
G12	PEG Smear Broad 22.5 v/v - L-Glutamic acid 0.05 M - Sodium chloride 0.3 M - L-Arginine 0.05 M - tris chloride BCC 0.1 M pH 7.5 🔍	pH 7.5	150 μl of Sodium chloride (2 M) 50 μl of L-Glutamic acid (1 M) 50 μl of L-Arginine (1 M) 100 μl of tris chloride BCC pH 7.5 (1 M) 450 μl of PEG Smear Broad (50 v/v) 200 μl of water (100 v/v)
H1	PEG Smear Low 25 v/v - Calcium chloride 0.04 M - Sodium formate 0.04 M - tris chloride BCC 0.1 M pH 8 🔍	pH 8	100 μl of tris chloride BCC pH 8 (1 M) 10 μl of Sodium formate (4 M) 40 μl of Calcium chloride (1 M) 500 μl of PEG Smear Low (50 v/v) 350 μl of water (100 v/v)
H2	PEG Smear Low 20 v/v - Magnesium chloride 0.1 M - Rubidium chloride 0.1 M - PIPES 0.1 M pH 7 🔍	pH 7	100 μl of Rubidium chloride (1 M) 100 μl of PIPES pH 7 (1 M) 100 μl of Magnesium chloride (1 M) 400 μl of PEG Smear Low (50 v/v) 300 μl of water (100 v/v)
H3	PEG Smear Medium 15 v/v - 2-Propanol 5 v/v - Magnesium chloride 0.2 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍	pH 7.5	50 μl of 2-Propanol (100 v/v) 100 μl of Magnesium chloride (2 M) 200 μl of Ethylene glycol (50 v/v) 300 μl of PEG Smear Medium (50 v/v) 100 μl of HEPES pH 7.5 (1 M) 250 μl of water (100 v/v)
H4	PEG Smear Medium 12 v/v - Ammonium acetate 0.05 M - Magnesium sulfate 0.15 M - HEPES 0.1 M pH 7 🔍	pH 7	50 μl of Ammonium acetate (1 M) 100 μl of HEPES pH 7 (1 M) 150 μl of Magnesium sulfate (1 M) 240 μl of PEG Smear Medium (50 v/v) 460 μl of water (100 v/v)
H5	PEG Smear Medium 20 v/v - HEPES 0.1 M pH 7.2 T-Mate 7 v/v - 🔍	pH 7.2	48 μl of HEPES pH 7.3 (1 M) 51 μl of HEPES pH 7.1 (1 M) 400 μl of PEG Smear Medium (50 v/v) 70 μl of T-Mate (100 v/v) 430 μl of water (100 v/v)
H6	PEG Smear High 15 v/v - Ammonium acetate 0.1 M - Zinc chloride 0.1 M - BIS-TRIS 0.1 M pH 7.2 🔍	pH 7.2	100 μl of Ammonium acetate (1 M) 300 μl of PEG Smear High (50 v/v) 49 μl of BIS-TRIS pH 7.5 (1 M) 50 μl of BIS-TRIS pH 7 (1 M) 100 μl of Zinc chloride (1 M) 400 μl of water (100 v/v)
H7	PEG Smear High 20 v/v - Lithium sulfate 0.15 M - Magnesium chloride 0.05 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100 μl of HEPES pH 7.8 (1 M) 400 μl of PEG Smear High (50 v/v) 150 μl of Lithium sulfate (1 M) 50 μl of Magnesium chloride (1 M) 300 μl of water (100 v/v)
H8	PEG Smear Broad 25 v/v - Potassium thiocyanate 100 mM - Sodium bromide 0.1 M - tris chloride BCC 0.1 M pH 7.8 🔍	pH 7.8	20 μl of Sodium bromide (5 M) 25 μl of tris chloride BCC pH 8.5 (1 M) 74 μl of tris chloride BCC pH 7.5 (1 M) 500 μl of PEG Smear Broad (50 v/v) 200 μl of Potassium thiocyanate (500 mM) 180 μl of water (100 v/v)
H9	PEG Smear Broad 28 v/v - Ammonium sulfate 0.05 M - Lithium sulfate 0.05 M - BIS-TRIS propane 0.1 M pH 8.5 🔍	pH 8.5	50 μl of Ammonium sulfate (1 M) 50 μl of Lithium sulfate (1 M) 100 μl of BIS-TRIS propane pH 8.5 (1 M) 560 μl of PEG Smear Broad (50 v/v) 240 μl of water (100 v/v)
H10	PEG Smear Broad 15 v/v - Ethylene glycol 10 v/v - Ammonium sulfate 0.2 M - Cadmium chloride 0.01 M - PIPES 0.1 M pH 7 🔍	pH 7	200 μl of Ammonium sulfate (1 M) 100 μl of Ethylene glycol (100 v/v) 100 μl of PIPES pH 7 (1 M) 100 μl of Cadmium chloride (0.1 M) 300 μl of PEG Smear Broad (50 v/v) 200 μl of water (100 v/v)
H11	PEG Smear Broad 22.5 v/v - Lithium sulfate 0.2 M - Zinc acetate 0.05 M - BIS-TRIS 0.1 M pH 7.5 🔍	pH 7.5	50 μl of Zinc acetate (1 M) 100 μl of BIS-TRIS pH 7.5 (1 M) 200 μl of Lithium sulfate (1 M) 450 μl of PEG Smear Broad (50 v/v) 200 μl of water (100 v/v)
H12	PEG Smear Broad 22.5 v/v - Sodium iodide 80 mM - Sodium bromide 0.08 M - Sodium fluoride 0.05 M - HEPES 0.1 M pH 7.8 🔍	pH 7.8	100 μl of HEPES pH 7.8 (1 M) 16 μl of Sodium bromide (5 M) 160 μl of Sodium iodide (500 mM) 25 μl of Sodium fluoride (2 M) 450 μl of PEG Smear Broad (50 v/v) 249 μl of water (100 v/v)