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Supplemental Information

Screen Description

MemTrans

Created 2018-08-29.

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MemTrans

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Screen Structure

MemTrans

Well Count 96
Number of distinct ingredients 63
Internal diversity (score mean dist) 0.923

Screen Composition

2025-08-16T09:05:41.194508 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemTrans
Buffer 119
Organic (non-volatile) 42
Polymer 98
Salt 143

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemTrans Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Ammonium thiocyanate 0.4 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 4.3
  • Polyethylene glycol 4,000 10 w/v -
pH 4.3
400.0 μl of Ammonium thiocyanate (1 M)
200.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
100.0 μl of tri-Sodium citrate pH 4.3 (1 M)
280.0 μl of water (100 v/v)
A2
  • Lithium sulfate 0.1 M -
  • Polyethylene glycol 400 26 v/v -
  • ADA 0.1 M pH 5.2
pH 5.2
92.2 μl of ADA pH 5 (1 M)
7.8 μl of ADA pH 6 (1 M)
50.0 μl of Lithium sulfate (2 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
590.0 μl of water (100 v/v)
A3
  • Potassium nitrate 0.14 M -
  • MES 0.1 M pH 7.1
  • Polyethylene glycol 3,350 14 w/v -
pH 7.1
81.8 μl of MES pH 7.4 (1 M)
18.2 μl of MES pH 6.5 (1 M)
280.0 μl of Polyethylene glycol 3,350 (50 w/v)
70.0 μl of Potassium nitrate (2 M)
550.0 μl of water (100 v/v)
A4
  • Sodium chloride 0.125 M -
  • Calcium chloride 0.04 M -
  • MES 0.1 M pH 5.5
  • Polyethylene glycol 400 22 v/v -
pH 5.5
40.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 5.5 (1 M)
440.0 μl of Polyethylene glycol 400 (50 v/v)
125.0 μl of Sodium chloride (1 M)
295.0 μl of water (100 v/v)
A5
  • Sodium chloride 0.05 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 200 30 v/v -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium chloride (1 M)
600.0 μl of water (100 v/v)
A6
  • Glycine 0.1 M pH 9
  • Polyethylene glycol monomethyl ether 350 38 v/v -
pH 9
100.0 μl of Glycine pH 9 (1 M)
380.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
520.0 μl of water (100 v/v)
A7
  • Calcium chloride 0.06 M -
  • Magnesium chloride 0.06 M -
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 4,000 25 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
60.0 μl of Calcium chloride (1 M)
60.0 μl of Magnesium chloride (1 M)
250.0 μl of Polyethylene glycol 4,000 (100 w/v)
530.0 μl of water (100 v/v)
A8
  • Ammonium sulfate 0.2 M -
  • Sodium chloride 0.02 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 400 24 v/v -
pH 4
200.0 μl of Ammonium sulfate (1 M)
240.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
20.0 μl of Sodium chloride (1 M)
440.0 μl of water (100 v/v)
A9
  • Potassium thiocyanate 0.2 M -
  • Glycine 0.1 M pH 9
  • Polyethylene glycol 3,350 8 w/v -
pH 9
100.0 μl of Glycine pH 9 (1 M)
160.0 μl of Polyethylene glycol 3,350 (50 w/v)
25.0 μl of Potassium thiocyanate (8 M)
715.0 μl of water (100 v/v)
A10
  • Sodium sulfate 0.15 M -
  • tris chloride BCC 0.025 M pH 7
  • Polyethylene glycol 400 29 v/v -
pH 7
290.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of Sodium sulfate (1 M)
25.0 μl of tris chloride BCC pH 7 (1 M)
535.0 μl of water (100 v/v)
A11
  • Sodium chloride 0.2 M -
  • MES 0.05 M pH 6.2
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 6.2
42.1 μl of MES pH 6.5 (0.5 M)
57.9 μl of MES pH 6 (0.5 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
360.0 μl of water (100 v/v)
A12
  • Calcium chloride 0.1 M -
  • Sodium cacodylate 0.05 M pH 5.5
  • Polyethylene glycol 400 29 v/v -
pH 5.5
100.0 μl of Calcium chloride (1 M)
580.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Sodium cacodylate pH 5.5 (1 M)
270.0 μl of water (100 v/v)
B1
  • Sodium chloride 0.115 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol monomethyl ether 550 18 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
360.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
115.0 μl of Sodium chloride (1 M)
425.0 μl of water (100 v/v)
B2
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 2,000 10 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
700.0 μl of water (100 v/v)
B3
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 600 36 v/v -
pH 7
100.0 μl of MOPS pH 7 (1 M)
360.0 μl of Polyethylene glycol 600 (100 v/v)
540.0 μl of water (100 v/v)
B4
  • tri-Potassium citrate 0.075 M -
  • MES 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 550 21 w/v -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
420.0 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
50.0 μl of tri-Potassium citrate (1.5 M)
430.0 μl of water (100 v/v)
B5
  • Ammonium sulfate 0.2 M -
  • Sodium cacodylate 0.1 M pH 5
  • Polyethylene glycol 400 24 v/v -
pH 5
200.0 μl of Ammonium sulfate (1 M)
480.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of Sodium cacodylate pH 5 (1 M)
220.0 μl of water (100 v/v)
B6
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 4,000 12 w/v -
pH 5
100.0 μl of Magnesium chloride (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
460.0 μl of water (100 v/v)
B7
  • Magnesium chloride 0.225 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 2,000 20 w/v -
pH 8.5
112.5 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
387.5 μl of water (100 v/v)
B8
  • Magnesium chloride 0.2 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 4,000 8 w/v -
pH 6.5
50.0 μl of MES pH 6.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
690.0 μl of water (100 v/v)
B9
  • Ammonium formate 0.1 M -
  • MES 0.1 M pH 6.2
  • Polyethylene glycol 400 25 v/v -
pH 6.2
40.0 μl of Ammonium formate (2.5 M)
100.0 μl of MES pH 6.2 (1 M)
500.0 μl of Polyethylene glycol 400 (50 v/v)
360.0 μl of water (100 v/v)
B10
  • tris chloride BCC 0.05 M pH 8.7
  • Polyethylene glycol 400 28 v/v -
pH 8.7
560.0 μl of Polyethylene glycol 400 (50 v/v)
24.3 μl of tris chloride BCC pH 9 (1 M)
25.7 μl of tris chloride BCC pH 8.5 (1 M)
390.0 μl of water (100 v/v)
B11
  • Sodium chloride 0.1 M -
  • ADA 0.05 M pH 6.5
  • Polyethylene glycol 400 25 v/v -
pH 6.5
50.0 μl of ADA pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Sodium chloride (2 M)
400.0 μl of water (100 v/v)
B12
  • Calcium chloride 0.055 M -
  • Potassium chloride 0.04 M -
  • MOPS 0.05 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 5 w/v -
pH 7
55.0 μl of Calcium chloride (1 M)
50.0 μl of MOPS pH 7 (1 M)
100.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
40.0 μl of Potassium chloride (1 M)
755.0 μl of water (100 v/v)
C1
  • Sodium acetate 0.03 M -
  • Sodium chloride 0.02 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 24 v/v -
pH 8
240.0 μl of Polyethylene glycol 400 (100 v/v)
15.0 μl of Sodium acetate (2 M)
20.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
625.0 μl of water (100 v/v)
C2
  • Magnesium chloride 0.25 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 2,000 23 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (0.5 M)
125.0 μl of Magnesium chloride (2 M)
460.0 μl of Polyethylene glycol 2,000 (50 w/v)
315.0 μl of water (100 v/v)
C3
  • Sodium chloride 0.06 M -
  • tri-Sodium citrate 0.05 M pH 4.5
  • Polyethylene glycol 400 24 v/v -
pH 4.5
240.0 μl of Polyethylene glycol 400 (100 v/v)
60.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 4.5 (1 M)
650.0 μl of water (100 v/v)
C4
  • Magnesium nitrate 0.1 M -
  • tris chloride BCC 0.05 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
pH 7
100.0 μl of Magnesium nitrate (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
50.0 μl of tris chloride BCC pH 7 (1 M)
550.0 μl of water (100 v/v)
C5
  • Magnesium chloride 0.01 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
5.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
595.0 μl of water (100 v/v)
C6
  • tri-Lithium citrate 0.05 M -
  • MOPS 0.05 M pH 6.5
  • Polyethylene glycol 1,000 30 w/v -
pH 6.5
50.0 μl of MOPS pH 6.5 (1 M)
600.0 μl of Polyethylene glycol 1,000 (50 w/v)
33.3 μl of tri-Lithium citrate (1.5 M)
316.7 μl of water (100 v/v)
C7
  • Lithium Phosphate 0.05 M -
  • Lithium sulfate 0.05 M -
  • Sodium acetate 0.025 M pH 5
  • Polyethylene glycol 300 32 v/v -
pH 5
50.0 μl of Lithium Phosphate (1 M)
50.0 μl of Lithium sulfate (1 M)
320.0 μl of Polyethylene glycol 300 (100 v/v)
25.0 μl of Sodium acetate pH 5 (1 M)
555.0 μl of water (100 v/v)
C8
  • tri-Sodium citrate 0.05 M -
  • tri-Potassium citrate 0.05 M -
  • ADA 0.05 M pH 6.8
  • Polyethylene glycol 400 25 v/v -
pH 6.8
50.0 μl of ADA pH 6.8 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of tri-Potassium citrate (1.5 M)
50.0 μl of tri-Sodium citrate (1 M)
616.7 μl of water (100 v/v)
C9
  • Nickel sulfate 0.05 M -
  • Lithium sulfate 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
50.0 μl of Lithium sulfate (1 M)
12.5 μl of Nickel sulfate (4 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
587.5 μl of water (100 v/v)
C10
  • Sodium chloride 0.2 M -
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 10 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
500.0 μl of water (100 v/v)
C11
  • Lithium nitrate 0.05 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
6.2 μl of Lithium nitrate (8 M)
600.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
293.8 μl of water (100 v/v)
C12
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 6.2
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 6.2
100.0 μl of MES pH 6.2 (1 M)
100.0 μl of Magnesium chloride (2 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
460.0 μl of water (100 v/v)
D1
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.03 M pH 8
  • Polyethylene glycol 4,000 17 w/v -
pH 8
100.0 μl of Magnesium chloride (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
30.0 μl of tris chloride BCC pH 8 (1 M)
530.0 μl of water (100 v/v)
D2
  • Calcium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 300 28 v/v -
pH 8.5
50.0 μl of Calcium chloride (2 M)
280.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
570.0 μl of water (100 v/v)
D3
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
600.0 μl of water (100 v/v)
D4
  • Sodium chloride 0.1 M -
  • Magnesium chloride 0.1 M -
  • MES 0.1 M pH 6.2
  • Polyethylene glycol 300 30 v/v -
pH 6.2
100.0 μl of MES pH 6.2 (1 M)
100.0 μl of Magnesium chloride (1 M)
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium chloride (1 M)
400.0 μl of water (100 v/v)
D5
  • Polyethylene glycol 400 17 v/v -
  • tri-Potassium citrate 0.1 M pH 5.4
pH 5.4
340.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of tri-Potassium citrate pH 5.4 (1 M)
560.0 μl of water (100 v/v)
D6
  • Lithium sulfate 0.11 M -
  • Sodium Phosphate Monobasic 0.04 M -
  • tri-Sodium citrate 0.05 M pH 4.3
  • Polyethylene glycol 1,000 15 w/v -
pH 4.3
110.0 μl of Lithium sulfate (1 M)
300.0 μl of Polyethylene glycol 1,000 (50 w/v)
40.0 μl of Sodium Phosphate Monobasic (1 M)
50.0 μl of tri-Sodium citrate pH 4.3 (1 M)
500.0 μl of water (100 v/v)
D7
  • Sodium chloride 0.1 M -
  • Calcium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 36 v/v -
pH 6.5
50.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (1 M)
390.0 μl of water (100 v/v)
D8
  • Zinc chloride 0.12 M -
  • tri-Potassium citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 40 v/v -
pH 4.2
400.0 μl of Polyethylene glycol 300 (100 v/v)
120.0 μl of Zinc chloride (1 M)
90.9 μl of tri-Potassium citrate pH 4 (1 M)
9.1 μl of tri-Potassium citrate pH 5.4 (1 M)
380.0 μl of water (100 v/v)
D9
  • Lithium nitrate 0.1 M -
  • Glycine 0.1 M pH 9.5
  • Polyethylene glycol 400 45 v/v -
pH 9.5
100.0 μl of Glycine pH 9.5 (1 M)
12.5 μl of Lithium nitrate (8 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
437.5 μl of water (100 v/v)
D10
  • tri-Sodium citrate 0.06 M -
  • Sodium chloride 0.05 M -
  • HEPES 0.05 M pH 8
  • Polyethylene glycol monomethyl ether 550 28 v/v -
pH 8
50.0 μl of HEPES pH 8 (1 M)
280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of Sodium chloride (1 M)
37.5 μl of tri-Sodium citrate (1.6 M)
582.5 μl of water (100 v/v)
D11
  • Calcium acetate 0.1 M -
  • Lithium sulfate 0.02 M -
  • tris chloride BCC 0.1 M pH 7.2
  • Polyethylene glycol 400 32 v/v -
pH 7.2
100.0 μl of Calcium acetate (1 M)
20.0 μl of Lithium sulfate (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
69.9 μl of tris chloride BCC pH 7 (1 M)
30.1 μl of tris chloride BCC pH 7.5 (1 M)
460.0 μl of water (100 v/v)
D12
  • Praseodymium (III) acetate 0.01 M -
  • Magnesium acetate 0.05 M -
  • Sodium acetate 0.05 M pH 5.8
  • Polyethylene glycol 400 25 v/v -
pH 5.8
50.0 μl of Magnesium acetate (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Praseodymium (III) acetate (0.1 M)
23.3 μl of Sodium acetate pH 6.2 (1 M)
26.7 μl of Sodium acetate pH 5.6 (1 M)
550.0 μl of water (100 v/v)
E1
  • Ammonium acetate 0.2 M -
  • Sodium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6.3
  • Polyethylene glycol 4,000 12 w/v -
pH 6.3
200.0 μl of Ammonium acetate (1 M)
56.4 μl of BIS-TRIS pH 6.5 (1 M)
43.6 μl of BIS-TRIS pH 6 (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium chloride (1 M)
360.0 μl of water (100 v/v)
E2
  • Potassium chloride 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Pentaerythritol Propoxylate (5/4 PO/OH) 27 v/v -
  • Polyethylene glycol 400 8 v/v -
pH 5.5
540.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
80.0 μl of Polyethylene glycol 400 (100 v/v)
200.0 μl of Potassium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
80.0 μl of water (100 v/v)
E3
  • Calcium acetate 0.1 M -
  • Sodium chloride 0.1 M -
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 400 24 v/v -
pH 7
100.0 μl of Calcium acetate (1 M)
100.0 μl of MOPS pH 7 (1 M)
480.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of Sodium chloride (1 M)
220.0 μl of water (100 v/v)
E4
  • Magnesium chloride 0.2 M -
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 2,000 21 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
200.0 μl of Magnesium chloride (1 M)
420.0 μl of Polyethylene glycol 2,000 (50 w/v)
280.0 μl of water (100 v/v)
E5
  • Lithium sulfate 0.075 M -
  • Lithium Phosphate 0.075 M -
  • MOPS 0.1 M pH 7.4
  • Polyethylene glycol monomethyl ether 550 24 v/v -
pH 7.4
75.0 μl of Lithium Phosphate (1 M)
75.0 μl of Lithium sulfate (1 M)
81.0 μl of MOPS pH 7.5 (1 M)
19.0 μl of MOPS pH 7 (1 M)
480.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
270.0 μl of water (100 v/v)
E6
  • Calcium chloride 0.045 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 8,000 5 w/v -
pH 7
45.0 μl of Calcium chloride (1 M)
100.0 μl of HEPES pH 7 (1 M)
125.0 μl of Polyethylene glycol 8,000 (40 w/v)
730.0 μl of water (100 v/v)
E7
  • Barium chloride 0.15 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 26 v/v -
pH 7
150.0 μl of Barium chloride (1 M)
100.0 μl of HEPES pH 7 (1 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
490.0 μl of water (100 v/v)
E8
  • Sodium chloride 0.125 M -
  • Lithium sulfate 0.125 M -
  • HEPES 0.1 M pH 8
  • Polyethylene glycol 300 33 v/v -
pH 8
100.0 μl of HEPES pH 8 (1 M)
125.0 μl of Lithium sulfate (1 M)
330.0 μl of Polyethylene glycol 300 (100 v/v)
125.0 μl of Sodium chloride (1 M)
320.0 μl of water (100 v/v)
E9
  • Magnesium nitrate 0.1 M -
  • Magnesium acetate 0.05 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 1,500 18 w/v -
pH 5
50.0 μl of Magnesium acetate (1 M)
100.0 μl of Magnesium nitrate (1 M)
360.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
390.0 μl of water (100 v/v)
E10
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.08 M pH 9
  • Polyethylene glycol 600 17 v/v -
pH 9
226.7 μl of Polyethylene glycol 600 (75 v/v)
100.0 μl of Sodium chloride (1 M)
80.0 μl of tris chloride BCC pH 9 (1 M)
593.3 μl of water (100 v/v)
E11
  • Nickel (II) chloride 0.01 M -
  • Sodium cacodylate 0.05 M pH 5.5
  • Polyethylene glycol 400 45 v/v -
pH 5.5
10.0 μl of Nickel (II) chloride (1 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium cacodylate pH 5.5 (1 M)
490.0 μl of water (100 v/v)
E12
  • Sodium chloride 0.4 M -
  • tri-Sodium citrate 0.05 M pH 6
  • Polyethylene glycol 400 37 v/v -
pH 6
370.0 μl of Polyethylene glycol 400 (100 v/v)
80.0 μl of Sodium chloride (5 M)
11.2 μl of tri-Sodium citrate pH 5.9 (1 M)
38.8 μl of tri-Sodium citrate pH 6.2 (1 M)
500.0 μl of water (100 v/v)
F1
  • Lithium sulfate 0.2 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 400 20 v/v -
pH 6.5
100.0 μl of Lithium sulfate (2 M)
50.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
650.0 μl of water (100 v/v)
F2
  • Sodium chloride 0.075 M -
  • CHES 0.05 M pH 9.5
  • Polyethylene glycol 300 32 v/v -
pH 9.5
50.0 μl of CHES pH 9.5 (1 M)
320.0 μl of Polyethylene glycol 300 (100 v/v)
75.0 μl of Sodium chloride (1 M)
555.0 μl of water (100 v/v)
F3
  • Sodium chloride 0.4 M -
  • tris chloride BCC 0.05 M pH 8
  • Polyethylene glycol 600 26 v/v -
pH 8
260.0 μl of Polyethylene glycol 600 (100 v/v)
200.0 μl of Sodium chloride (2 M)
50.0 μl of tris chloride BCC pH 8 (1 M)
490.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 3,350 15 w/v -
  • di-Sodium succinate 0.1 M pH 7
pH 7
300.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium succinate pH 7 (1 M)
600.0 μl of water (100 v/v)
F5
  • Calcium acetate 0.08 M -
  • Sodium chloride 0.05 M -
  • MOPS 0.1 M pH 7
  • Polyethylene glycol 400 28 v/v -
pH 7
80.0 μl of Calcium acetate (1 M)
100.0 μl of MOPS pH 7 (1 M)
560.0 μl of Polyethylene glycol 400 (50 v/v)
50.0 μl of Sodium chloride (1 M)
210.0 μl of water (100 v/v)
F6
  • Sodium chloride 0.07 M -
  • tri-Sodium citrate 0.05 M pH 4.5
  • Polyethylene glycol 400 22 v/v -
  • Polyethylene glycol 600 2 v/v -
pH 4.5
220.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Polyethylene glycol 600 (50 v/v)
70.0 μl of Sodium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 4.5 (1 M)
620.0 μl of water (100 v/v)
F7
  • di-Sodium malonate 0.2 M -
  • HEPES 0.075 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7
75.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
66.7 μl of di-Sodium malonate (3 M)
458.3 μl of water (100 v/v)
F8
  • Potassium di-hydrogen phosphate 0.08 M -
  • ADA 0.05 M pH 6.5
  • Polyethylene glycol 400 35 v/v -
pH 6.5
50.0 μl of ADA pH 6.5 (1 M)
700.0 μl of Polyethylene glycol 400 (50 v/v)
80.0 μl of Potassium di-hydrogen phosphate (1 M)
170.0 μl of water (100 v/v)
F9
  • Lithium sulfate 0.1 M -
  • HEPES 0.05 M pH 7
  • Polyethylene glycol 3,350 31 w/v -
pH 7
50.0 μl of HEPES pH 7 (1 M)
100.0 μl of Lithium sulfate (1 M)
620.0 μl of Polyethylene glycol 3,350 (50 w/v)
230.0 μl of water (100 v/v)
F10
  • Potassium acetate 0.25 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 300 24 v/v -
pH 8
240.0 μl of Polyethylene glycol 300 (100 v/v)
50.0 μl of Potassium acetate (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
610.0 μl of water (100 v/v)
F11
  • Magnesium acetate 0.125 M -
  • Magnesium chloride 0.04 M -
  • tris chloride BCC 0.08 M pH 8.7
  • Polyethylene glycol 4,000 12 w/v -
pH 8.7
125.0 μl of Magnesium acetate (1 M)
40.0 μl of Magnesium chloride (1 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
41.1 μl of tris chloride BCC pH 8.5 (1 M)
38.9 μl of tris chloride BCC pH 9 (1 M)
515.0 μl of water (100 v/v)
F12
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.7
  • Polyethylene glycol monomethyl ether 550 38 v/v -
pH 8.7
100.0 μl of Magnesium chloride (1 M)
380.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
48.6 μl of tris chloride BCC pH 9 (1 M)
51.4 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
G1
  • Nickel (II) chloride 0.05 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol monomethyl ether 2,000 17 w/v -
pH 4
50.0 μl of Nickel (II) chloride (1 M)
340.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
510.0 μl of water (100 v/v)
G2
  • Magnesium chloride 0.05 M -
  • Sodium cacodylate 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
pH 5.5
50.0 μl of Magnesium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium cacodylate pH 5.5 (1 M)
450.0 μl of water (100 v/v)
G3
  • Sodium chloride 0.15 M -
  • MOPS 0.075 M pH 7
  • Polyethylene glycol monomethyl ether 2,000 20 w/v -
pH 7
75.0 μl of MOPS pH 7 (1 M)
400.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
150.0 μl of Sodium chloride (1 M)
375.0 μl of water (100 v/v)
G4
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 300 30 v/v -
  • 2-methyl-2,4-pentanediol BCC 3 v/v -
pH 4.5
200.0 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
300.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
G5
  • Ammonium sulfate 0.1 M -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 400 22 v/v -
pH 7.5
100.0 μl of Ammonium sulfate (1 M)
50.0 μl of HEPES pH 7.5 (1 M)
440.0 μl of Polyethylene glycol 400 (50 v/v)
410.0 μl of water (100 v/v)
G6
  • Ammonium sulfate 0.2 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 25 v/v -
pH 6.5
66.7 μl of Ammonium sulfate (3 M)
100.0 μl of MES pH 6.5 (1 M)
500.0 μl of Polyethylene glycol 400 (50 v/v)
333.3 μl of water (100 v/v)
G7
  • Calcium chloride 0.12 M -
  • BIS-TRIS 0.075 M pH 6.3
  • Polyethylene glycol monomethyl ether 2,000 15 w/v -
pH 6.3
42.3 μl of BIS-TRIS pH 6.5 (1 M)
32.7 μl of BIS-TRIS pH 6 (1 M)
120.0 μl of Calcium chloride (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
505.0 μl of water (100 v/v)
G8
  • Cadmium chloride 0.005 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 31 v/v -
pH 7.5
50.0 μl of Cadmium chloride (0.1 M)
100.0 μl of HEPES pH 7.5 (1 M)
620.0 μl of Polyethylene glycol 400 (50 v/v)
230.0 μl of water (100 v/v)
G9
  • Potassium chloride 0.1 M -
  • MES 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 550 22 v/v -
pH 5
100.0 μl of MES pH 5 (1 M)
440.0 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
100.0 μl of Potassium chloride (1 M)
360.0 μl of water (100 v/v)
G10
  • Lithium chloride 0.1 M -
  • Glycine 0.05 M pH 9.5
  • Polyethylene glycol 400 40 v/v -
pH 9.5
25.0 μl of Glycine pH 9.5 (2 M)
100.0 μl of Lithium chloride (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
475.0 μl of water (100 v/v)
G11
  • tri-Sodium citrate 0.05 M -
  • Potassium di-hydrogen phosphate 0.08 M -
  • BIS-TRIS 0.08 M pH 6.3
  • Polyethylene glycol 4,000 11 w/v -
  • Potassium chloride 0.06 M -
pH 6.3
34.8 μl of BIS-TRIS pH 6 (1 M)
45.2 μl of BIS-TRIS pH 6.5 (1 M)
220.0 μl of Polyethylene glycol 4,000 (50 w/v)
60.0 μl of Potassium chloride (1 M)
80.0 μl of Potassium di-hydrogen phosphate (1 M)
31.2 μl of tri-Sodium citrate (1.6 M)
528.8 μl of water (100 v/v)
G12
  • Calcium chloride 0.05 M -
  • tris chloride BCC 0.02 M pH 7.4
  • Polyethylene glycol 3,000 14 w/v -
pH 7.4
50.0 μl of Calcium chloride (1 M)
280.0 μl of Polyethylene glycol 3,000 (50 w/v)
5.4 μl of tris chloride BCC pH 7 (1 M)
14.6 μl of tris chloride BCC pH 7.5 (1 M)
650.0 μl of water (100 v/v)
H1
  • Zinc sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 4,000 15 w/v -
pH 4.5
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
200.0 μl of Zinc sulfate (1 M)
400.0 μl of water (100 v/v)
H2
  • Sodium chloride 0.3 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 21 v/v -
pH 5.5
420.0 μl of Polyethylene glycol 400 (50 v/v)
300.0 μl of Sodium chloride (1 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
180.0 μl of water (100 v/v)
H3
  • Magnesium chloride 0.08 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 1,500 19 w/v -
pH 8.5
80.0 μl of Magnesium chloride (1 M)
380.0 μl of Polyethylene glycol 1,500 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
440.0 μl of water (100 v/v)
H4
  • Sodium chloride 0.125 M -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 34 v/v -
pH 8.5
50.0 μl of Magnesium chloride (2 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
385.0 μl of water (100 v/v)
H5
  • Magnesium chloride 0.05 M -
  • Potassium chloride 0.02 M -
  • Sodium cacodylate 0.05 M pH 5.8
  • Polyethylene glycol 200 40 v/v -
pH 5.8
50.0 μl of Magnesium chloride (1 M)
400.0 μl of Polyethylene glycol 200 (100 v/v)
20.0 μl of Potassium chloride (1 M)
50.0 μl of Sodium cacodylate pH 5.8 (1 M)
480.0 μl of water (100 v/v)
H6
  • Ammonium acetate 0.2 M -
  • tris chloride BCC 0.05 M pH 8.5
  • Polyethylene glycol 3,350 24 w/v -
pH 8.5
200.0 μl of Ammonium acetate (1 M)
480.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
270.0 μl of water (100 v/v)
H7
  • Lithium sulfate 0.08 M -
  • Sodium Phosphate Monobasic 0.06 M -
  • tri-Sodium citrate 0.05 M pH 4.7
  • Polyethylene glycol 1,000 16 w/v -
pH 4.7
80.0 μl of Lithium sulfate (1 M)
320.0 μl of Polyethylene glycol 1,000 (50 w/v)
60.0 μl of Sodium Phosphate Monobasic (1 M)
50.0 μl of tri-Sodium citrate pH 4.7 (1 M)
490.0 μl of water (100 v/v)
H8
  • Potassium thiocyanate 125 mM -
  • Calcium chloride 0.01 M -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 4,000 8 w/v -
pH 7.5
100.0 μl of Calcium chloride (0.1 M)
50.0 μl of HEPES pH 7.5 (1 M)
160.0 μl of Polyethylene glycol 4,000 (50 w/v)
250.0 μl of Potassium thiocyanate (500 mM)
440.0 μl of water (100 v/v)
H9
  • Magnesium nitrate 0.06 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol monomethyl ether 550 24 v/v -
pH 5.5
60.0 μl of Magnesium nitrate (1 M)
240.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
600.0 μl of water (100 v/v)
H10
  • Sodium chloride 80 mM -
  • Lithium sulfate 25 mM -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 400 27 v/v -
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
250.0 μl of Lithium sulfate (100 mM)
270.0 μl of Polyethylene glycol 400 (100 v/v)
160.0 μl of Sodium chloride (500 mM)
270.0 μl of water (100 v/v)
H11
  • Sodium chloride 0.5 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 40 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
166.7 μl of Sodium chloride (3 M)
333.3 μl of water (100 v/v)
H12
  • Ammonium di-hydrogen phosphate 0.2 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 1,500 20 w/v -
pH 7
200.0 μl of Ammonium di-hydrogen phosphate (1 M)
100.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 1,500 (50 w/v)
300.0 μl of water (100 v/v)