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Supplemental Information

Screen Description

MemTrans

Created 2018-08-29.

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MemTrans

    Screen Structure

    MemTrans

    Well Count 96
    Number of distinct ingredients 63
    Internal diversity (score mean dist) 0.923

    Screen Composition

    2025-06-13T21:31:48.818427 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemTrans
    Buffer 119
    Organic (non-volatile) 42
    Polymer 98
    Salt 143

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemTrans Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium thiocyanate 0.4 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 4.3
    • Polyethylene glycol 4,000 10 w/v -
    pH 4.3
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of Sodium chloride (5 M)
    100 μl of tri-Sodium citrate pH 4.3 (1 M)
    400 μl of Ammonium thiocyanate (1 M)
    280 μl of water (100 v/v)
    A2
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 26 v/v -
    • ADA 0.1 M pH 5.2
    pH 5.2
    50 μl of Lithium sulfate (2 M)
    92 μl of ADA pH 5 (1 M)
    7 μl of ADA pH 6 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    590 μl of water (100 v/v)
    A3
    • Potassium nitrate 0.14 M -
    • MES 0.1 M pH 7.1
    • Polyethylene glycol 3,350 14 w/v -
    pH 7.1
    18 μl of MES pH 6.5 (1 M)
    81 μl of MES pH 7.4 (1 M)
    280 μl of Polyethylene glycol 3,350 (50 w/v)
    70 μl of Potassium nitrate (2 M)
    550 μl of water (100 v/v)
    A4
    • Sodium chloride 0.125 M -
    • Calcium chloride 0.04 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 400 22 v/v -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    125 μl of Sodium chloride (1 M)
    440 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of Calcium chloride (1 M)
    295 μl of water (100 v/v)
    A5
    • Sodium chloride 0.05 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 200 30 v/v -
    pH 6.5
    50 μl of Sodium chloride (1 M)
    50 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    600 μl of water (100 v/v)
    A6
    • Glycine 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 350 38 v/v -
    pH 9
    100 μl of Glycine pH 9 (1 M)
    380 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    520 μl of water (100 v/v)
    A7
    • Calcium chloride 0.06 M -
    • Magnesium chloride 0.06 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    60 μl of Calcium chloride (1 M)
    60 μl of Magnesium chloride (1 M)
    100 μl of BICINE pH 8.5 (1 M)
    250 μl of Polyethylene glycol 4,000 (100 w/v)
    530 μl of water (100 v/v)
    A8
    • Ammonium sulfate 0.2 M -
    • Sodium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 400 24 v/v -
    pH 4
    20 μl of Sodium chloride (1 M)
    100 μl of Sodium acetate pH 4 (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    200 μl of Ammonium sulfate (1 M)
    440 μl of water (100 v/v)
    A9
    • Potassium thiocyanate 0.2 M -
    • Glycine 0.1 M pH 9
    • Polyethylene glycol 3,350 8 w/v -
    pH 9
    100 μl of Glycine pH 9 (1 M)
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    715 μl of water (100 v/v)
    A10
    • Sodium sulfate 0.15 M -
    • tris chloride BCC 0.025 M pH 7
    • Polyethylene glycol 400 29 v/v -
    pH 7
    290 μl of Polyethylene glycol 400 (100 v/v)
    150 μl of Sodium sulfate (1 M)
    25 μl of tris chloride BCC pH 7 (1 M)
    535 μl of water (100 v/v)
    A11
    • Sodium chloride 0.2 M -
    • MES 0.05 M pH 6.2
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 6.2
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Sodium chloride (1 M)
    42 μl of MES pH 6.5 (0.5 M)
    57 μl of MES pH 6 (0.5 M)
    360 μl of water (100 v/v)
    A12
    • Calcium chloride 0.1 M -
    • Sodium cacodylate 0.05 M pH 5.5
    • Polyethylene glycol 400 29 v/v -
    pH 5.5
    580 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Calcium chloride (1 M)
    50 μl of Sodium cacodylate pH 5.5 (1 M)
    270 μl of water (100 v/v)
    B1
    • Sodium chloride 0.115 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 18 v/v -
    pH 7
    360 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    115 μl of Sodium chloride (1 M)
    425 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 2,000 10 w/v -
    pH 5.5
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    700 μl of water (100 v/v)
    B3
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 600 36 v/v -
    pH 7
    100 μl of MOPS pH 7 (1 M)
    360 μl of Polyethylene glycol 600 (100 v/v)
    540 μl of water (100 v/v)
    B4
    • tri-Potassium citrate 0.075 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 21 w/v -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    50 μl of tri-Potassium citrate (1.5 M)
    420 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    430 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.2 M -
    • Sodium cacodylate 0.1 M pH 5
    • Polyethylene glycol 400 24 v/v -
    pH 5
    480 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Ammonium sulfate (1 M)
    220 μl of water (100 v/v)
    B6
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 4,000 12 w/v -
    pH 5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    100 μl of Sodium chloride (1 M)
    100 μl of Magnesium chloride (1 M)
    460 μl of water (100 v/v)
    B7
    • Magnesium chloride 0.225 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 2,000 20 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    112 μl of Magnesium chloride (2 M)
    387 μl of water (100 v/v)
    B8
    • Magnesium chloride 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 6.5
    50 μl of MES pH 6.5 (1 M)
    100 μl of Magnesium chloride (2 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    690 μl of water (100 v/v)
    B9
    • Ammonium formate 0.1 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol 400 25 v/v -
    pH 6.2
    100 μl of MES pH 6.2 (1 M)
    40 μl of Ammonium formate (2.5 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    360 μl of water (100 v/v)
    B10
    • tris chloride BCC 0.05 M pH 8.7
    • Polyethylene glycol 400 28 v/v -
    pH 8.7
    25 μl of tris chloride BCC pH 8.5 (1 M)
    24 μl of tris chloride BCC pH 9 (1 M)
    560 μl of Polyethylene glycol 400 (50 v/v)
    390 μl of water (100 v/v)
    B11
    • Sodium chloride 0.1 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 400 25 v/v -
    pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of Sodium chloride (2 M)
    400 μl of water (100 v/v)
    B12
    • Calcium chloride 0.055 M -
    • Potassium chloride 0.04 M -
    • MOPS 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 5 w/v -
    pH 7
    40 μl of Potassium chloride (1 M)
    50 μl of MOPS pH 7 (1 M)
    55 μl of Calcium chloride (1 M)
    100 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    755 μl of water (100 v/v)
    C1
    • Sodium acetate 0.03 M -
    • Sodium chloride 0.02 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 24 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    15 μl of Sodium acetate (2 M)
    20 μl of Sodium chloride (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    625 μl of water (100 v/v)
    C2
    • Magnesium chloride 0.25 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 2,000 23 w/v -
    pH 6.5
    125 μl of Magnesium chloride (2 M)
    100 μl of MES pH 6.5 (0.5 M)
    460 μl of Polyethylene glycol 2,000 (50 w/v)
    315 μl of water (100 v/v)
    C3
    • Sodium chloride 0.06 M -
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 400 24 v/v -
    pH 4.5
    240 μl of Polyethylene glycol 400 (100 v/v)
    60 μl of Sodium chloride (1 M)
    50 μl of tri-Sodium citrate pH 4.5 (1 M)
    650 μl of water (100 v/v)
    C4
    • Magnesium nitrate 0.1 M -
    • tris chloride BCC 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 7
    50 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Magnesium nitrate (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    550 μl of water (100 v/v)
    C5
    • Magnesium chloride 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    5 μl of Magnesium chloride (2 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    595 μl of water (100 v/v)
    C6
    • tri-Lithium citrate 0.05 M -
    • MOPS 0.05 M pH 6.5
    • Polyethylene glycol 1,000 30 w/v -
    pH 6.5
    50 μl of MOPS pH 6.5 (1 M)
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    33 μl of tri-Lithium citrate (1.5 M)
    316 μl of water (100 v/v)
    C7
    • Lithium Phosphate 0.05 M -
    • Lithium sulfate 0.05 M -
    • Sodium acetate 0.025 M pH 5
    • Polyethylene glycol 300 32 v/v -
    pH 5
    25 μl of Sodium acetate pH 5 (1 M)
    320 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Lithium sulfate (1 M)
    50 μl of Lithium Phosphate (1 M)
    555 μl of water (100 v/v)
    C8
    • tri-Sodium citrate 0.05 M -
    • tri-Potassium citrate 0.05 M -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 400 25 v/v -
    pH 6.8
    33 μl of tri-Potassium citrate (1.5 M)
    50 μl of tri-Sodium citrate (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of ADA pH 6.8 (1 M)
    616 μl of water (100 v/v)
    C9
    • Nickel sulfate 0.05 M -
    • Lithium sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    12 μl of Nickel sulfate (4 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Lithium sulfate (1 M)
    587 μl of water (100 v/v)
    C10
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 9.5
    200 μl of Sodium chloride (1 M)
    100 μl of CHES pH 9.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    C11
    • Lithium nitrate 0.05 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 6.5
    6 μl of Lithium nitrate (8 M)
    100 μl of ADA pH 6.5 (1 M)
    600 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    293 μl of water (100 v/v)
    C12
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 6.2
    100 μl of MES pH 6.2 (1 M)
    100 μl of Magnesium chloride (2 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    460 μl of water (100 v/v)
    D1
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.03 M pH 8
    • Polyethylene glycol 4,000 17 w/v -
    pH 8
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (1 M)
    30 μl of tris chloride BCC pH 8 (1 M)
    530 μl of water (100 v/v)
    D2
    • Calcium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 300 28 v/v -
    pH 8.5
    50 μl of Calcium chloride (2 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    570 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    600 μl of water (100 v/v)
    D4
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol 300 30 v/v -
    pH 6.2
    100 μl of Sodium chloride (1 M)
    100 μl of MES pH 6.2 (1 M)
    100 μl of Magnesium chloride (1 M)
    300 μl of Polyethylene glycol 300 (100 v/v)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 17 v/v -
    • tri-Potassium citrate 0.1 M pH 5.4
    pH 5.4
    340 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of tri-Potassium citrate pH 5.4 (1 M)
    560 μl of water (100 v/v)
    D6
    • Lithium sulfate 0.11 M -
    • Sodium Phosphate Monobasic 0.04 M -
    • tri-Sodium citrate 0.05 M pH 4.3
    • Polyethylene glycol 1,000 15 w/v -
    pH 4.3
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of tri-Sodium citrate pH 4.3 (1 M)
    40 μl of Sodium Phosphate Monobasic (1 M)
    110 μl of Lithium sulfate (1 M)
    500 μl of water (100 v/v)
    D7
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 36 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    360 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride (1 M)
    50 μl of Calcium chloride (1 M)
    390 μl of water (100 v/v)
    D8
    • Zinc chloride 0.12 M -
    • tri-Potassium citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    pH 4.2
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of tri-Potassium citrate pH 4 (1 M)
    120 μl of Zinc chloride (1 M)
    0 μl of tri-Potassium citrate pH 5.4 (1 M)
    380 μl of water (100 v/v)
    D9
    • Lithium nitrate 0.1 M -
    • Glycine 0.1 M pH 9.5
    • Polyethylene glycol 400 45 v/v -
    pH 9.5
    100 μl of Glycine pH 9.5 (1 M)
    12 μl of Lithium nitrate (8 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    437 μl of water (100 v/v)
    D10
    • tri-Sodium citrate 0.06 M -
    • Sodium chloride 0.05 M -
    • HEPES 0.05 M pH 8
    • Polyethylene glycol monomethyl ether 550 28 v/v -
    pH 8
    50 μl of Sodium chloride (1 M)
    37 μl of tri-Sodium citrate (1.6 M)
    280 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    50 μl of HEPES pH 8 (1 M)
    582 μl of water (100 v/v)
    D11
    • Calcium acetate 0.1 M -
    • Lithium sulfate 0.02 M -
    • tris chloride BCC 0.1 M pH 7.2
    • Polyethylene glycol 400 32 v/v -
    pH 7.2
    69 μl of tris chloride BCC pH 7 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Lithium sulfate (1 M)
    30 μl of tris chloride BCC pH 7.5 (1 M)
    100 μl of Calcium acetate (1 M)
    460 μl of water (100 v/v)
    D12
    • Praseodymium (III) acetate 0.01 M -
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 5.8
    • Polyethylene glycol 400 25 v/v -
    pH 5.8
    100 μl of Praseodymium (III) acetate (0.1 M)
    23 μl of Sodium acetate pH 6.2 (1 M)
    26 μl of Sodium acetate pH 5.6 (1 M)
    50 μl of Magnesium acetate (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    E1
    • Ammonium acetate 0.2 M -
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.3
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.3
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (1 M)
    200 μl of Ammonium acetate (1 M)
    43 μl of BIS-TRIS pH 6 (1 M)
    56 μl of BIS-TRIS pH 6.5 (1 M)
    360 μl of water (100 v/v)
    E2
    • Potassium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 27 v/v -
    • Polyethylene glycol 400 8 v/v -
    pH 5.5
    540 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    200 μl of Potassium chloride (1 M)
    80 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of water (100 v/v)
    E3
    • Calcium acetate 0.1 M -
    • Sodium chloride 0.1 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 24 v/v -
    pH 7
    100 μl of Sodium chloride (1 M)
    480 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of MOPS pH 7 (1 M)
    100 μl of Calcium acetate (1 M)
    220 μl of water (100 v/v)
    E4
    • Magnesium chloride 0.2 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 2,000 21 w/v -
    pH 8.5
    200 μl of Magnesium chloride (1 M)
    420 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    280 μl of water (100 v/v)
    E5
    • Lithium sulfate 0.075 M -
    • Lithium Phosphate 0.075 M -
    • MOPS 0.1 M pH 7.4
    • Polyethylene glycol monomethyl ether 550 24 v/v -
    pH 7.4
    480 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    75 μl of Lithium sulfate (1 M)
    19 μl of MOPS pH 7 (1 M)
    75 μl of Lithium Phosphate (1 M)
    81 μl of MOPS pH 7.5 (1 M)
    270 μl of water (100 v/v)
    E6
    • Calcium chloride 0.045 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 8,000 5 w/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    45 μl of Calcium chloride (1 M)
    125 μl of Polyethylene glycol 8,000 (40 w/v)
    730 μl of water (100 v/v)
    E7
    • Barium chloride 0.15 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 26 v/v -
    pH 7
    150 μl of Barium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    490 μl of water (100 v/v)
    E8
    • Sodium chloride 0.125 M -
    • Lithium sulfate 0.125 M -
    • HEPES 0.1 M pH 8
    • Polyethylene glycol 300 33 v/v -
    pH 8
    125 μl of Sodium chloride (1 M)
    330 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of HEPES pH 8 (1 M)
    125 μl of Lithium sulfate (1 M)
    320 μl of water (100 v/v)
    E9
    • Magnesium nitrate 0.1 M -
    • Magnesium acetate 0.05 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 1,500 18 w/v -
    pH 5
    50 μl of Magnesium acetate (1 M)
    100 μl of Magnesium nitrate (1 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    360 μl of Polyethylene glycol 1,500 (50 w/v)
    390 μl of water (100 v/v)
    E10
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.08 M pH 9
    • Polyethylene glycol 600 17 v/v -
    pH 9
    80 μl of tris chloride BCC pH 9 (1 M)
    100 μl of Sodium chloride (1 M)
    226 μl of Polyethylene glycol 600 (75 v/v)
    593 μl of water (100 v/v)
    E11
    • Nickel (II) chloride 0.01 M -
    • Sodium cacodylate 0.05 M pH 5.5
    • Polyethylene glycol 400 45 v/v -
    pH 5.5
    10 μl of Nickel (II) chloride (1 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Sodium cacodylate pH 5.5 (1 M)
    490 μl of water (100 v/v)
    E12
    • Sodium chloride 0.4 M -
    • tri-Sodium citrate 0.05 M pH 6
    • Polyethylene glycol 400 37 v/v -
    pH 6
    48 μl of tri-Sodium citrate pH 6.2 (1 M)
    1 μl of tri-Sodium citrate pH 5.9 (1 M)
    80 μl of Sodium chloride (5 M)
    370 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    F1
    • Lithium sulfate 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 400 20 v/v -
    pH 6.5
    100 μl of Lithium sulfate (2 M)
    50 μl of MES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    650 μl of water (100 v/v)
    F2
    • Sodium chloride 0.075 M -
    • CHES 0.05 M pH 9.5
    • Polyethylene glycol 300 32 v/v -
    pH 9.5
    75 μl of Sodium chloride (1 M)
    50 μl of CHES pH 9.5 (1 M)
    320 μl of Polyethylene glycol 300 (100 v/v)
    555 μl of water (100 v/v)
    F3
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 600 26 v/v -
    pH 8
    50 μl of tris chloride BCC pH 8 (1 M)
    200 μl of Sodium chloride (2 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    490 μl of water (100 v/v)
    F4
    • Polyethylene glycol 3,350 15 w/v -
    • di-Sodium succinate 0.1 M pH 7
    pH 7
    100 μl of di-Sodium succinate pH 7 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    F5
    • Calcium acetate 0.08 M -
    • Sodium chloride 0.05 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 28 v/v -
    pH 7
    50 μl of Sodium chloride (1 M)
    100 μl of MOPS pH 7 (1 M)
    560 μl of Polyethylene glycol 400 (50 v/v)
    80 μl of Calcium acetate (1 M)
    210 μl of water (100 v/v)
    F6
    • Sodium chloride 0.07 M -
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 400 22 v/v -
    • Polyethylene glycol 600 2 v/v -
    pH 4.5
    70 μl of Sodium chloride (1 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of Polyethylene glycol 600 (50 v/v)
    50 μl of tri-Sodium citrate pH 4.5 (1 M)
    620 μl of water (100 v/v)
    F7
    • di-Sodium malonate 0.2 M -
    • HEPES 0.075 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    75 μl of HEPES pH 7 (1 M)
    66 μl of di-Sodium malonate (3 M)
    458 μl of water (100 v/v)
    F8
    • Potassium di-hydrogen phosphate 0.08 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 400 35 v/v -
    pH 6.5
    80 μl of Potassium di-hydrogen phosphate (1 M)
    50 μl of ADA pH 6.5 (1 M)
    700 μl of Polyethylene glycol 400 (50 v/v)
    170 μl of water (100 v/v)
    F9
    • Lithium sulfate 0.1 M -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 31 w/v -
    pH 7
    620 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    100 μl of Lithium sulfate (1 M)
    230 μl of water (100 v/v)
    F10
    • Potassium acetate 0.25 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 24 v/v -
    pH 8
    100 μl of tris chloride BCC pH 8 (1 M)
    240 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Potassium acetate (5 M)
    610 μl of water (100 v/v)
    F11
    • Magnesium acetate 0.125 M -
    • Magnesium chloride 0.04 M -
    • tris chloride BCC 0.08 M pH 8.7
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.7
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    41 μl of tris chloride BCC pH 8.5 (1 M)
    38 μl of tris chloride BCC pH 9 (1 M)
    125 μl of Magnesium acetate (1 M)
    40 μl of Magnesium chloride (1 M)
    515 μl of water (100 v/v)
    F12
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.7
    • Polyethylene glycol monomethyl ether 550 38 v/v -
    pH 8.7
    51 μl of tris chloride BCC pH 8.5 (1 M)
    380 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Magnesium chloride (1 M)
    48 μl of tris chloride BCC pH 9 (1 M)
    420 μl of water (100 v/v)
    G1
    • Nickel (II) chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 4
    50 μl of Nickel (II) chloride (1 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    510 μl of water (100 v/v)
    G2
    • Magnesium chloride 0.05 M -
    • Sodium cacodylate 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    pH 5.5
    50 μl of Magnesium chloride (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium cacodylate pH 5.5 (1 M)
    450 μl of water (100 v/v)
    G3
    • Sodium chloride 0.15 M -
    • MOPS 0.075 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    150 μl of Sodium chloride (1 M)
    75 μl of MOPS pH 7 (1 M)
    375 μl of water (100 v/v)
    G4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 30 v/v -
    • 2-methyl-2,4-pentanediol BCC 3 v/v -
    pH 4.5
    200 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    G5
    • Ammonium sulfate 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 22 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of HEPES pH 7.5 (1 M)
    100 μl of Ammonium sulfate (1 M)
    410 μl of water (100 v/v)
    G6
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 25 v/v -
    pH 6.5
    500 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of MES pH 6.5 (1 M)
    66 μl of Ammonium sulfate (3 M)
    333 μl of water (100 v/v)
    G7
    • Calcium chloride 0.12 M -
    • BIS-TRIS 0.075 M pH 6.3
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.3
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    32 μl of BIS-TRIS pH 6 (1 M)
    120 μl of Calcium chloride (1 M)
    42 μl of BIS-TRIS pH 6.5 (1 M)
    505 μl of water (100 v/v)
    G8
    • Cadmium chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 31 v/v -
    pH 7.5
    50 μl of Cadmium chloride (0.1 M)
    620 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    230 μl of water (100 v/v)
    G9
    • Potassium chloride 0.1 M -
    • MES 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 5
    100 μl of MES pH 5 (1 M)
    100 μl of Potassium chloride (1 M)
    440 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    360 μl of water (100 v/v)
    G10
    • Lithium chloride 0.1 M -
    • Glycine 0.05 M pH 9.5
    • Polyethylene glycol 400 40 v/v -
    pH 9.5
    25 μl of Glycine pH 9.5 (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium chloride (1 M)
    475 μl of water (100 v/v)
    G11
    • tri-Sodium citrate 0.05 M -
    • Potassium di-hydrogen phosphate 0.08 M -
    • BIS-TRIS 0.08 M pH 6.3
    • Polyethylene glycol 4,000 11 w/v -
    • Potassium chloride 0.06 M -
    pH 6.3
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    31 μl of tri-Sodium citrate (1.6 M)
    80 μl of Potassium di-hydrogen phosphate (1 M)
    45 μl of BIS-TRIS pH 6.5 (1 M)
    34 μl of BIS-TRIS pH 6 (1 M)
    60 μl of Potassium chloride (1 M)
    528 μl of water (100 v/v)
    G12
    • Calcium chloride 0.05 M -
    • tris chloride BCC 0.02 M pH 7.4
    • Polyethylene glycol 3,000 14 w/v -
    pH 7.4
    5 μl of tris chloride BCC pH 7 (1 M)
    50 μl of Calcium chloride (1 M)
    280 μl of Polyethylene glycol 3,000 (50 w/v)
    14 μl of tris chloride BCC pH 7.5 (1 M)
    650 μl of water (100 v/v)
    H1
    • Zinc sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 4.5
    200 μl of Zinc sulfate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    H2
    • Sodium chloride 0.3 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 21 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    420 μl of Polyethylene glycol 400 (50 v/v)
    300 μl of Sodium chloride (1 M)
    180 μl of water (100 v/v)
    H3
    • Magnesium chloride 0.08 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,500 19 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    380 μl of Polyethylene glycol 1,500 (50 w/v)
    80 μl of Magnesium chloride (1 M)
    440 μl of water (100 v/v)
    H4
    • Sodium chloride 0.125 M -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 34 v/v -
    pH 8.5
    125 μl of Sodium chloride (1 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    50 μl of Magnesium chloride (2 M)
    340 μl of Polyethylene glycol 400 (100 v/v)
    385 μl of water (100 v/v)
    H5
    • Magnesium chloride 0.05 M -
    • Potassium chloride 0.02 M -
    • Sodium cacodylate 0.05 M pH 5.8
    • Polyethylene glycol 200 40 v/v -
    pH 5.8
    50 μl of Sodium cacodylate pH 5.8 (1 M)
    20 μl of Potassium chloride (1 M)
    400 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Magnesium chloride (1 M)
    480 μl of water (100 v/v)
    H6
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 3,350 24 w/v -
    pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    480 μl of Polyethylene glycol 3,350 (50 w/v)
    270 μl of water (100 v/v)
    H7
    • Lithium sulfate 0.08 M -
    • Sodium Phosphate Monobasic 0.06 M -
    • tri-Sodium citrate 0.05 M pH 4.7
    • Polyethylene glycol 1,000 16 w/v -
    pH 4.7
    50 μl of tri-Sodium citrate pH 4.7 (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    60 μl of Sodium Phosphate Monobasic (1 M)
    80 μl of Lithium sulfate (1 M)
    490 μl of water (100 v/v)
    H8
    • Potassium thiocyanate 125 mM -
    • Calcium chloride 0.01 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 7.5
    250 μl of Potassium thiocyanate (500 mM)
    100 μl of Calcium chloride (0.1 M)
    50 μl of HEPES pH 7.5 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    440 μl of water (100 v/v)
    H9
    • Magnesium nitrate 0.06 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 24 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    60 μl of Magnesium nitrate (1 M)
    600 μl of water (100 v/v)
    H10
    • Sodium chloride 80 mM -
    • Lithium sulfate 25 mM -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 27 v/v -
    pH 7.5
    160 μl of Sodium chloride (500 mM)
    270 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of HEPES pH 7.5 (1 M)
    250 μl of Lithium sulfate (100 mM)
    270 μl of water (100 v/v)
    H11
    • Sodium chloride 0.5 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 40 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    166 μl of Sodium chloride (3 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    333 μl of water (100 v/v)
    H12
    • Ammonium di-hydrogen phosphate 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 1,500 20 w/v -
    pH 7
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of water (100 v/v)