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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
JCSG Top96
Barcodes
JCSG Top96
No barcodes found.
Screen Structure
JCSG Top96
Well Count | 96 |
---|---|
Number of distinct ingredients | 54 |
Internal diversity (score mean dist) | 0.948 |
Screen Composition
ingredient type | JCSG Top96 |
---|---|
Buffer | 100 |
Cryoprotectant | 12 |
Organic (non-volatile) | 29 |
Organic (volatile) | 4 |
Polymer | 74 |
Salt | 109 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 400.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of water (100 v/v) |
A2 |
| NA | 250.0 μl of Ethylene glycol (100 v/v) 750.0 μl of water (100 v/v) |
A3 |
| pH 2.5 | 333.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 70.8 μl of tri-Sodium citrate pH 1 (1 M) 29.2 μl of tri-Sodium citrate pH 3 (1 M) 566.7 μl of water (100 v/v) |
A4 |
| pH 5 | 333.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 566.7 μl of water (100 v/v) |
A5 |
| pH 6.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 6.5 (1 M) 500.0 μl of water (100 v/v) |
A6 |
| pH 8.5 | 650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 8.5 (1 M) 250.0 μl of water (100 v/v) |
A7 |
| pH 4.2 | 100.0 μl of Ammonium sulfate (2 M) 333.3 μl of Glycerol (30 v/v) 200.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 266.7 μl of water (100 v/v) |
A8 |
| pH 7.5 | 425.0 μl of Ammonium sulfate (4 M) 187.5 μl of Glycerol (80 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 113.3 μl of Polyethylene glycol 400 (15 v/v) 189.2 μl of water (100 v/v) |
A9 |
| pH 4.6 | 160.0 μl of Ammonium sulfate (1 M) 200.0 μl of Glycerol (100 v/v) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 360.0 μl of water (100 v/v) |
A10 |
| pH 5.6 | 170.0 μl of Ammonium acetate (1 M) 300.0 μl of Glycerol (50 v/v) 255.0 μl of Polyethylene glycol 4,000 (100 w/v) 85.0 μl of tri-Sodium citrate pH 5.6 (1 M) 190.0 μl of water (100 v/v) |
A11 |
| pH 8.5 | 187.5 μl of Glycerol (80 v/v) 255.0 μl of Polyethylene glycol 4,000 (100 w/v) 85.0 μl of Sodium acetate (2 M) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 387.5 μl of water (100 v/v) |
A12 |
| pH 6.5 | 200.0 μl of Glycerol (100 v/v) 160.0 μl of Magnesium acetate (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 240.0 μl of water (100 v/v) |
B1 |
| pH 7.5 | 125.0 μl of Glycerol (80 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 300.0 μl of Polyethylene glycol 400 (100 v/v) 375.0 μl of water (100 v/v) |
B2 |
| pH 6 | 200.0 μl of Glycerol (50 v/v) 100.0 μl of MES pH 6 (1 M) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of Polyethylene glycol 600 (75 w/v) 200.0 μl of water (100 v/v) |
B3 |
| pH 4.6 | 375.0 μl of Glycerol (80 v/v) 112.0 μl of Polyethylene glycol 4,000 (50 w/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 443.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 283.3 μl of 2-Propanol (30 v/v) 187.5 μl of Glycerol (80 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 340.0 μl of Polyethylene glycol 4,000 (50 w/v) 104.2 μl of water (100 v/v) |
B5 |
| pH 5.6 | 190.0 μl of 2-Propanol (100 v/v) 100.0 μl of Glycerol (50 v/v) 380.0 μl of Polyethylene glycol 4,000 (50 w/v) 95.0 μl of tri-Sodium citrate pH 5.6 (1 M) 235.0 μl of water (100 v/v) |
B6 |
| pH 7.5 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 400.0 μl of water (100 v/v) |
B7 |
| pH 5.6 | 200.0 μl of 2-Propanol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 300.0 μl of water (100 v/v) |
B8 |
| pH 6.5 | 400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v) |
B9 |
| pH 4.6 | 444.4 μl of Ammonium sulfate (3.6 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 275.6 μl of water (100 v/v) |
B10 |
| pH 4.6 | 300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of Sodium chloride (2 M) 500.0 μl of water (100 v/v) |
B11 |
| pH 5.6 | 66.7 μl of Ammonium acetate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 833.3 μl of water (100 v/v) |
B12 |
| pH 6 | 350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Lithium sulfate (2 M) 100.0 μl of MES pH 6 (1 M) 450.0 μl of water (100 v/v) |
C1 |
| pH 7 | 500.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 400.0 μl of water (100 v/v) |
C2 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 400.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v) |
C3 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 533.3 μl of Polyethylene glycol 600 (75 w/v) 366.7 μl of water (100 v/v) |
C4 |
| pH 5.5 | 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v) |
C5 |
| pH 4 | 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 800.0 μl of water (100 v/v) |
C6 |
| pH 4 | 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 500.0 μl of water (100 v/v) |
C7 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 500.0 μl of water (100 v/v) |
C8 |
| pH 5 | 100.0 μl of MES pH 5 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
C9 |
| pH 8.5 | 100.0 μl of BICINE pH 8.5 (1 M) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
C10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
C11 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
C12 |
| pH 3.5 | 266.7 μl of Ammonium sulfate (3 M) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 633.3 μl of water (100 v/v) |
D1 |
| pH 4 | 457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 442.9 μl of water (100 v/v) |
D2 |
| pH 5 | 457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of MES pH 5 (1 M) 442.9 μl of water (100 v/v) |
D3 |
| pH 9 | 457.1 μl of Ammonium sulfate (3.5 M) 100.0 μl of BICINE pH 9 (1 M) 442.9 μl of water (100 v/v) |
D4 |
| pH 6.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 40.0 μl of Sodium chloride (5 M) 288.6 μl of water (100 v/v) |
D5 |
| pH 8.5 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 328.6 μl of water (100 v/v) |
D6 |
| pH 10 | 571.4 μl of Ammonium sulfate (3.5 M) 100.0 μl of CAPS pH 10 (1 M) 66.7 μl of Lithium sulfate (3 M) 261.9 μl of water (100 v/v) |
D7 |
| pH 8.5 | 685.7 μl of Ammonium sulfate (3.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 214.3 μl of water (100 v/v) |
D8 |
| pH 9 | 685.7 μl of Ammonium sulfate (3.5 M) 100.0 μl of BICINE pH 9 (1 M) 214.3 μl of water (100 v/v) |
D9 |
| pH 4.5 | 100.0 μl of Lithium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of Sodium chloride (5 M) 300.0 μl of water (100 v/v) |
D10 |
| pH 6.5 | 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v) |
D11 |
| pH 7 | 100.0 μl of Sodium chloride (2 M) 500.0 μl of tri-Sodium citrate pH 6.5 (2 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 300.0 μl of water (100 v/v) |
D12 |
| pH 9.5 | 100.0 μl of CHES pH 9.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 275.0 μl of water (100 v/v) |
E1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 466.7 μl of tri-Sodium citrate (3 M) 433.3 μl of water (100 v/v) |
E2 |
| pH 10 | 100.0 μl of CAPS pH 10 (1 M) 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Sodium Phosphate Monobasic (3 M) 266.7 μl of di-Potassium hydrogen phosphate (3 M) 133.3 μl of water (100 v/v) |
E3 |
| pH 4.2 | 500.0 μl of Polyethylene glycol 200 (100 v/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 360.0 μl of water (100 v/v) |
E4 |
| pH 6.5 | 100.0 μl of Calcium acetate (2 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v) |
E5 |
| pH 7.5 | 100.0 μl of Calcium chloride (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 520.0 μl of water (100 v/v) |
E6 |
| pH 4.5 | 200.0 μl of Calcium acetate (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 400.0 μl of water (100 v/v) |
E7 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
E8 |
| pH 5.5 | 100.0 μl of MES pH 5.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v) |
E9 |
| pH 6.5 | 100.0 μl of Magnesium chloride (2 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
E10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Sodium chloride (2 M) 400.0 μl of water (100 v/v) |
E11 |
| pH 7 | 600.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 260.0 μl of water (100 v/v) |
E12 |
| NA | 20.0 μl of Ammonium fluoride (10 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 580.0 μl of water (100 v/v) |
F1 |
| NA | 80.0 μl of Ammonium formate (2.5 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 520.0 μl of water (100 v/v) |
F2 |
| NA | 200.0 μl of Ammonium sulfate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F3 |
| NA | 200.0 μl of Calcium acetate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F4 |
| NA | 100.0 μl of Calcium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F5 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 57.1 μl of di-Ammonium hydrogen phosphate (3.5 M) 542.9 μl of water (100 v/v) |
F6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Ammonium tartrate (2 M) 500.0 μl of water (100 v/v) |
F7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of di-Sodium hydrogen phosphate (2 M) 500.0 μl of water (100 v/v) |
F8 |
| NA | 100.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F9 |
| NA | 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 500.0 μl of water (100 v/v) |
F10 |
| NA | 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F11 |
| NA | 200.0 μl of Magnesium nitrate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
F12 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Potassium formate (2 M) 500.0 μl of water (100 v/v) |
G1 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Potassium nitrate (2 M) 500.0 μl of water (100 v/v) |
G2 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate (2 M) 500.0 μl of water (100 v/v) |
G3 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium fluoride (2 M) 500.0 μl of water (100 v/v) |
G4 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Sodium formate (4 M) 550.0 μl of water (100 v/v) |
G5 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.6 μl of Sodium nitrate (7 M) 571.4 μl of water (100 v/v) |
G6 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium thiocyanate (2 M) 500.0 μl of water (100 v/v) |
G7 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Ammonium citrate (2.5 M) 520.0 μl of water (100 v/v) |
G8 |
| NA | 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.3 μl of tri-Potassium citrate (1.5 M) 466.7 μl of water (100 v/v) |
G9 |
| pH 4.6 | 100.0 μl of Ammonium sulfate (2 M) 250.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 550.0 μl of water (100 v/v) |
G10 |
| pH 5.6 | 200.0 μl of Ammonium acetate (1 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v) |
G11 |
| pH 8.5 | 100.0 μl of Lithium sulfate (2 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
G12 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v) |
H1 |
| pH 8.5 | 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
H2 |
| pH 5 | 200.0 μl of Lithium chloride (5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
H3 |
| pH 8.5 | 200.0 μl of Lithium chloride (5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
H4 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
H5 |
| pH 6 | 200.0 μl of Lithium chloride (5 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 300.0 μl of water (100 v/v) |
H6 |
| pH 5 | 200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
H7 |
| pH 8 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 500.0 μl of water (100 v/v) |
H8 |
| pH 6 | 200.0 μl of Calcium acetate (1 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 300.0 μl of water (100 v/v) |
H9 |
| pH 6.5 | 200.0 μl of Magnesium acetate (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 300.0 μl of water (100 v/v) |
H10 |
| pH 8.5 | 80.0 μl of Magnesium chloride (2.5 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 420.0 μl of water (100 v/v) |
H11 |
| pH 10 | 100.0 μl of CAPS pH 10 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of Sodium chloride (1 M) 300.0 μl of water (100 v/v) |
H12 |
| pH 6.5 | 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 200.0 μl of water (100 v/v) |