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Supplemental Information

Screen Description

Rigaku / JCSG Top96
Created 2018-10-02.

Rigaku Recipe XML

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Screen Structure

Well Count 96
Number of distinct ingredients 54
Internal diversity (Score mean dist)0.95
Internal diversity (Ion mean dist)0.92
Internal diversity (PEG mean dist)0.91
Internal diversity (Known pH mean dist)0.85
Internal diversity (Penalty mean dist)0.92

Screen Composition

Chemical classes bar chart
ingredient type JCSG Top96
Buffer 100
Cryoprotectant 12
Organic (non-volatile) 29
Organic (volatile) 4
Polymer 74
Salt 109

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Top96 Well Contents  🔍
Well Ingredient, Concentration, Units, pH pHRecipe 1000μl
A1
  • 1,2-Propanediol 40 v/v -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
A2
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
A3
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • tri-Sodium citrate 0.1 M pH 2.5
pH 2.5
333.33 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
95.11 μl of tri-Sodium citrate pH 1 (1 M)
4.89 μl of tri-Sodium citrate pH 3 (1 M)
566.67 μl of water (100 v/v)
A4
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
  • Sodium acetate 0.1 M pH 5
pH 5
333.33 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
566.67 μl of water (100 v/v)
A5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • HEPES 0.1 M pH 6.5
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
A6
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 8.5 (1 M)
250.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 300 20 v/v -
  • Ammonium sulfate 0.2 M -
  • Glycerol 10 v/v -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
100.0 μl of Ammonium sulfate (2 M)
333.33 μl of Glycerol (30 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
266.67 μl of water (100 v/v)
A8
  • Ammonium sulfate 1.7 M -
  • Polyethylene glycol 400 1.7 v/v -
  • Glycerol 15 v/v -
  • HEPES 0.085 M pH 7.5
pH 7.5
425.0 μl of Ammonium sulfate (4 M)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
113.33 μl of Polyethylene glycol 400 (15 v/v)
189.17 μl of water (100 v/v)
A9
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.16 M -
  • Glycerol 20 v/v -
  • Sodium acetate 0.08 M pH 4.6
pH 4.6
160.0 μl of Ammonium sulfate (1 M)
200.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
360.0 μl of water (100 v/v)
A10
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium acetate 0.17 M -
  • Glycerol 15 v/v -
  • tri-Sodium citrate 0.085 M pH 5.6
pH 5.6
170.0 μl of Ammonium acetate (1 M)
300.0 μl of Glycerol (50 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
190.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
  • Sodium acetate 0.17 M -
  • tris chloride BCC 0.085 M pH 8.5
pH 8.5
187.5 μl of Glycerol (80 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of Sodium acetate (2 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
387.5 μl of water (100 v/v)
A12
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
  • Magnesium acetate 0.16 M -
  • Sodium cacodylate 0.08 M pH 6.5
pH 6.5
200.0 μl of Glycerol (100 v/v)
160.0 μl of Magnesium acetate (1 M)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
240.0 μl of water (100 v/v)
B1
  • Polyethylene glycol 400 30 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • Glycerol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
125.0 μl of Glycerol (80 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
375.0 μl of water (100 v/v)
B2
  • Polyethylene glycol 600 30 w/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Glycerol 10 v/v -
  • MES 0.1 M pH 6
pH 6
200.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
200.0 μl of water (100 v/v)
B3
  • Glycerol 30 v/v -
  • Polyethylene glycol 4,000 5.6 w/v -
  • Sodium acetate 0.07 M pH 4.6
pH 4.6
375.0 μl of Glycerol (80 v/v)
112.0 μl of Polyethylene glycol 4,000 (50 w/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
443.0 μl of water (100 v/v)
B4
  • Polyethylene glycol 4,000 17 w/v -
  • 2-Propanol 8.5 v/v -
  • Glycerol 15 v/v -
  • HEPES 0.085 M pH 7.5
pH 7.5
283.33 μl of 2-Propanol (30 v/v)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
104.17 μl of water (100 v/v)
B5
  • 2-Propanol 19 v/v -
  • Polyethylene glycol 4,000 19 w/v -
  • Glycerol 5 v/v -
  • tri-Sodium citrate 0.095 M pH 5.6
pH 5.6
190.0 μl of 2-Propanol (100 v/v)
100.0 μl of Glycerol (50 v/v)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
95.0 μl of tri-Sodium citrate pH 5.6 (1 M)
235.0 μl of water (100 v/v)
B6
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
B7
  • 2-Propanol 20 v/v -
  • Polyethylene glycol 4,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B9
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
  • Sodium acetate 0.08 M pH 4.6
pH 4.6
444.44 μl of Ammonium sulfate (3.6 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
275.56 μl of water (100 v/v)
B10
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium chloride 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of Sodium chloride (2 M)
500.0 μl of water (100 v/v)
B11
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
66.67 μl of Ammonium acetate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
833.33 μl of water (100 v/v)
B12
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of MES pH 6 (1 M)
450.0 μl of water (100 v/v)
C1
  • Polyethylene glycol 200 50 v/v -
  • tris chloride BCC 0.1 M pH 7
pH 7
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
400.0 μl of water (100 v/v)
C2
  • Polyethylene glycol 400 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • MES 0.1 M pH 6
pH 6
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
C3
  • Polyethylene glycol 600 40 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
533.33 μl of Polyethylene glycol 600 (75 w/v)
366.67 μl of water (100 v/v)
C4
  • Polyethylene glycol 3,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
C5
  • Polyethylene glycol 6,000 5 w/v -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
800.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 6,000 20 w/v -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
500.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 6,000 20 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 6,000 30 w/v -
  • MES 0.1 M pH 5
pH 5
100.0 μl of MES pH 5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 6,000 30 w/v -
  • BICINE 0.1 M pH 8.5
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 8,000 20 w/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 8,000 20 w/v -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
C12
  • Ammonium sulfate 0.8 M -
  • tri-Sodium citrate 0.1 M pH 3.5
pH 3.5
266.67 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
633.33 μl of water (100 v/v)
D1
  • Ammonium sulfate 1.6 M -
  • tri-Sodium citrate 0.1 M pH 4
pH 4
457.14 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
442.86 μl of water (100 v/v)
D2
  • Ammonium sulfate 1.6 M -
  • MES 0.1 M pH 5
pH 5
457.14 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 5 (1 M)
442.86 μl of water (100 v/v)
D3
  • Ammonium sulfate 1.6 M -
  • BICINE 0.1 M pH 9
pH 9
457.14 μl of Ammonium sulfate (3.5 M)
100.0 μl of BICINE pH 9 (1 M)
442.86 μl of water (100 v/v)
D4
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
288.57 μl of water (100 v/v)
D5
  • Ammonium sulfate 2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.57 μl of water (100 v/v)
D6
  • Ammonium sulfate 2 M -
  • Lithium sulfate 0.2 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
571.43 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10.5 (1 M)
66.67 μl of Lithium sulfate (3 M)
261.9 μl of water (100 v/v)
D7
  • Ammonium sulfate 2.4 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
685.71 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
214.29 μl of water (100 v/v)
D8
  • Ammonium sulfate 2.4 M -
  • BICINE 0.1 M pH 9
pH 9
685.71 μl of Ammonium sulfate (3.5 M)
100.0 μl of BICINE pH 9 (1 M)
214.29 μl of water (100 v/v)
D9
  • Sodium chloride 2.5 M -
  • Lithium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
D10
  • tri-Sodium citrate 1 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
D11
  • tri-Sodium citrate 1 M -
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
100.0 μl of Sodium chloride (2 M)
500.0 μl of tri-Sodium citrate pH 6.5 (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
300.0 μl of water (100 v/v)
D12
  • tri-Sodium citrate 1 M -
  • CHES 0.1 M pH 9.5
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
E1
  • tri-Sodium citrate 1.4 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
466.67 μl of tri-Sodium citrate (3 M)
433.33 μl of water (100 v/v)
E2
  • Sodium Phosphate Monobasic 1.2 M -
  • Lithium sulfate 0.2 M -
  • di-Potassium hydrogen phosphate 0.8 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
266.67 μl of di-Potassium hydrogen phosphate (3 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Sodium Phosphate Monobasic (3 M)
133.33 μl of water (100 v/v)
E3
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
pH 4.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
360.0 μl of water (100 v/v)
E4
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Calcium acetate (2 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 30 v/v -
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 400 40 v/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E8
  • Polyethylene glycol 400 40 v/v -
  • Magnesium chloride 0.2 M -
  • MES 0.1 M pH 5.5
pH 5.5
100.0 μl of Magnesium chloride (2 M)
100.0 μl of MES pH 5.5 (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E9
  • Polyethylene glycol 3,000 10 w/v -
  • Magnesium chloride 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
E10
  • Polyethylene glycol 3,000 20 w/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium chloride (2 M)
400.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 3,000 30 w/v -
  • Sodium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium fluoride 0.2 M -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
F1
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium formate 0.2 M -
NA
80.0 μl of Ammonium formate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
F2
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium sulfate 0.2 M -
NA
200.0 μl of Ammonium sulfate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 3,350 20 w/v -
  • Calcium acetate 0.2 M -
NA
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 3,350 20 w/v -
  • Calcium chloride 0.2 M -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F5
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium hydrogen phosphate 0.2 M -
NA
57.14 μl of di-Ammonium hydrogen phosphate (3.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
542.86 μl of water (100 v/v)
F6
  • Polyethylene glycol 3,350 20 w/v -
  • di-Ammonium tartrate 0.2 M -
NA
100.0 μl of di-Ammonium tartrate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 3,350 20 w/v -
  • di-Sodium hydrogen phosphate 0.2 M -
NA
100.0 μl of di-Sodium hydrogen phosphate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F8
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium acetate 0.2 M -
NA
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F9
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium chloride 0.2 M -
NA
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium formate 0.2 M -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 3,350 20 w/v -
  • Magnesium nitrate 0.2 M -
NA
200.0 μl of Magnesium nitrate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium formate (2 M)
500.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 3,350 20 w/v -
  • Potassium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium nitrate (2 M)
500.0 μl of water (100 v/v)
G2
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium acetate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate (2 M)
500.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium fluoride 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium fluoride (2 M)
500.0 μl of water (100 v/v)
G4
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium formate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium formate (4 M)
550.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium nitrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.57 μl of Sodium nitrate (7 M)
571.43 μl of water (100 v/v)
G6
  • Polyethylene glycol 3,350 20 w/v -
  • Sodium thiocyanate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium thiocyanate (2 M)
500.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Ammonium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
520.0 μl of water (100 v/v)
G8
  • Polyethylene glycol 3,350 20 w/v -
  • tri-Potassium citrate 0.2 M -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.33 μl of tri-Potassium citrate (1.5 M)
466.67 μl of water (100 v/v)
G9
  • Polyethylene glycol 4,000 25 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium acetate 0.1 M pH 4.6
pH 4.6
100.0 μl of Ammonium sulfate (2 M)
250.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 4,000 30 w/v -
  • Ammonium acetate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.6
pH 5.6
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 4,000 30 w/v -
  • Lithium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 4,000 30 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 4,000 30 w/v -
  • Sodium acetate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 6,000 10 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 6,000 10 w/v -
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H4
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • MES 0.1 M pH 6
pH 6
200.0 μl of Lithium chloride (5 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 6,000 20 w/v -
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
pH 5
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 8,000 10 w/v -
  • Calcium acetate 0.2 M -
  • Imidazole 0.1 M pH 8
pH 8
200.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 8,000 20 w/v -
  • Calcium acetate 0.2 M -
  • MES 0.1 M pH 6
pH 6
200.0 μl of Calcium acetate (1 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
300.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 8,000 20 w/v -
  • Magnesium chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium chloride 0.2 M -
  • CAPS 0.1 M pH 10.5
pH 10.5
100.0 μl of CAPS pH 10.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium acetate 0.2 M -
  • Sodium cacodylate 0.1 M pH 6.5
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of water (100 v/v)