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Supplemental Information

Screen Description

JCSG Top96

Rigaku / JCSG Top96
Created 2018-10-02.

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JCSG Top96

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Screen Structure

JCSG Top96

Well Count 96
Number of distinct ingredients 54
Internal diversity (score mean dist) 0.948

Screen Composition

2025-08-16T06:25:38.450815 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG Top96
Buffer 100
Cryoprotectant 12
Organic (non-volatile) 29
Organic (volatile) 4
Polymer 74
Salt 109

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Top96 Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
A2
  • Ethylene glycol 25 v/v -
NA
250.0 μl of Ethylene glycol (100 v/v)
750.0 μl of water (100 v/v)
A3
  • tri-Sodium citrate 0.1 M pH 2.5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 2.5
333.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
70.8 μl of tri-Sodium citrate pH 1 (1 M)
29.2 μl of tri-Sodium citrate pH 3 (1 M)
566.7 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 10 v/v -
pH 5
333.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
566.7 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
A6
  • BICINE 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 8.5 (1 M)
250.0 μl of water (100 v/v)
A7
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 20 v/v -
  • Ammonium sulfate 0.2 M -
  • Glycerol 10 v/v -
pH 4.2
100.0 μl of Ammonium sulfate (2 M)
333.3 μl of Glycerol (30 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
266.7 μl of water (100 v/v)
A8
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 400 1.7 v/v -
  • Ammonium sulfate 1.7 M -
  • Glycerol 15 v/v -
pH 7.5
425.0 μl of Ammonium sulfate (4 M)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
113.3 μl of Polyethylene glycol 400 (15 v/v)
189.2 μl of water (100 v/v)
A9
  • Sodium acetate 0.08 M pH 4.6
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.16 M -
  • Glycerol 20 v/v -
pH 4.6
160.0 μl of Ammonium sulfate (1 M)
200.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
360.0 μl of water (100 v/v)
A10
  • tri-Sodium citrate 0.085 M pH 5.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium acetate 0.17 M -
  • Glycerol 15 v/v -
pH 5.6
170.0 μl of Ammonium acetate (1 M)
300.0 μl of Glycerol (50 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
190.0 μl of water (100 v/v)
A11
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
  • Sodium acetate 0.17 M -
pH 8.5
187.5 μl of Glycerol (80 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of Sodium acetate (2 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
387.5 μl of water (100 v/v)
A12
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
  • Magnesium acetate 0.16 M -
pH 6.5
200.0 μl of Glycerol (100 v/v)
160.0 μl of Magnesium acetate (1 M)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
240.0 μl of water (100 v/v)
B1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • Glycerol 10 v/v -
pH 7.5
125.0 μl of Glycerol (80 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
375.0 μl of water (100 v/v)
B2
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 w/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Glycerol 10 v/v -
pH 6
200.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
200.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.07 M pH 4.6
  • Polyethylene glycol 4,000 5.6 w/v -
  • Glycerol 30 v/v -
pH 4.6
375.0 μl of Glycerol (80 v/v)
112.0 μl of Polyethylene glycol 4,000 (50 w/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
443.0 μl of water (100 v/v)
B4
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 4,000 17 w/v -
  • 2-Propanol 8.5 v/v -
  • Glycerol 15 v/v -
pH 7.5
283.3 μl of 2-Propanol (30 v/v)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
104.2 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.095 M pH 5.6
  • Polyethylene glycol 4,000 19 w/v -
  • 2-Propanol 19 v/v -
  • Glycerol 5 v/v -
pH 5.6
190.0 μl of 2-Propanol (100 v/v)
100.0 μl of Glycerol (50 v/v)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
95.0 μl of tri-Sodium citrate pH 5.6 (1 M)
235.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
400.0 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
300.0 μl of water (100 v/v)
B8
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B9
  • Sodium acetate 0.08 M pH 4.6
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
pH 4.6
444.4 μl of Ammonium sulfate (3.6 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
275.6 μl of water (100 v/v)
B10
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium chloride 0.2 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of Sodium chloride (2 M)
500.0 μl of water (100 v/v)
B11
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
66.7 μl of Ammonium acetate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
833.3 μl of water (100 v/v)
B12
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Lithium sulfate 0.2 M -
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of MES pH 6 (1 M)
450.0 μl of water (100 v/v)
C1
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 200 50 v/v -
pH 7
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
400.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
C3
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 600 40 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
533.3 μl of Polyethylene glycol 600 (75 w/v)
366.7 μl of water (100 v/v)
C4
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
pH 5.5
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
C5
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 5 w/v -
pH 4
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
800.0 μl of water (100 v/v)
C6
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
pH 4
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
500.0 μl of water (100 v/v)
C7
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 20 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
500.0 μl of water (100 v/v)
C8
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 30 w/v -
pH 5
100.0 μl of MES pH 5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
C9
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 30 w/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
C10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
C11
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.1 M pH 3.5
  • Ammonium sulfate 0.8 M -
pH 3.5
266.7 μl of Ammonium sulfate (3 M)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
633.3 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 1.6 M -
pH 4
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
442.9 μl of water (100 v/v)
D2
  • MES 0.1 M pH 5
  • Ammonium sulfate 1.6 M -
pH 5
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 5 (1 M)
442.9 μl of water (100 v/v)
D3
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 1.6 M -
pH 9
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BICINE pH 9 (1 M)
442.9 μl of water (100 v/v)
D4
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
288.6 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
328.6 μl of water (100 v/v)
D6
  • CAPS 0.1 M pH 10
  • Ammonium sulfate 2 M -
  • Lithium sulfate 0.2 M -
pH 10
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10 (1 M)
66.7 μl of Lithium sulfate (3 M)
261.9 μl of water (100 v/v)
D7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2.4 M -
pH 8.5
685.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
214.3 μl of water (100 v/v)
D8
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2.4 M -
pH 9
685.7 μl of Ammonium sulfate (3.5 M)
100.0 μl of BICINE pH 9 (1 M)
214.3 μl of water (100 v/v)
D9
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
  • Sodium chloride 2.5 M -
pH 4.5
100.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of Sodium chloride (5 M)
300.0 μl of water (100 v/v)
D10
  • Sodium cacodylate 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
pH 6.5
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 7
  • Sodium chloride 0.2 M -
  • tri-Sodium citrate 1 M -
pH 7
100.0 μl of Sodium chloride (2 M)
500.0 μl of tri-Sodium citrate pH 6.5 (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
300.0 μl of water (100 v/v)
D12
  • CHES 0.1 M pH 9.5
  • tri-Sodium citrate 1 M -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
275.0 μl of water (100 v/v)
E1
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
466.7 μl of tri-Sodium citrate (3 M)
433.3 μl of water (100 v/v)
E2
  • CAPS 0.1 M pH 10
  • Lithium sulfate 0.2 M -
  • di-Potassium hydrogen phosphate 0.8 M -
  • Sodium Phosphate Monobasic 1.2 M -
pH 10
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Sodium Phosphate Monobasic (3 M)
266.7 μl of di-Potassium hydrogen phosphate (3 M)
133.3 μl of water (100 v/v)
E3
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
pH 4.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
360.0 μl of water (100 v/v)
E4
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.2 M -
pH 6.5
100.0 μl of Calcium acetate (2 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
E5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
520.0 μl of water (100 v/v)
E6
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
  • Calcium acetate 0.2 M -
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
E7
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 40 v/v -
  • Lithium sulfate 0.2 M -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
E8
  • MES 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
  • Magnesium chloride 0.2 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E9
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 3,000 10 w/v -
  • Magnesium chloride 0.2 M -
pH 6.5
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
  • Sodium chloride 0.2 M -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium chloride (2 M)
400.0 μl of water (100 v/v)
E11
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,000 30 w/v -
  • Sodium chloride 0.2 M -
pH 7
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E12
  • Ammonium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
20.0 μl of Ammonium fluoride (10 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
580.0 μl of water (100 v/v)
F1
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
80.0 μl of Ammonium formate (2.5 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
520.0 μl of water (100 v/v)
F2
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Ammonium sulfate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F3
  • Calcium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Calcium acetate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F4
  • Calcium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Calcium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F5
  • di-Ammonium hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
542.9 μl of water (100 v/v)
F6
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
500.0 μl of water (100 v/v)
F7
  • di-Sodium hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium hydrogen phosphate (2 M)
500.0 μl of water (100 v/v)
F8
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F9
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
500.0 μl of water (100 v/v)
F10
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F11
  • Magnesium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
200.0 μl of Magnesium nitrate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F12
  • Potassium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium formate (2 M)
500.0 μl of water (100 v/v)
G1
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium nitrate (2 M)
500.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate (2 M)
500.0 μl of water (100 v/v)
G3
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium fluoride (2 M)
500.0 μl of water (100 v/v)
G4
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium formate (4 M)
550.0 μl of water (100 v/v)
G5
  • Sodium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium nitrate (7 M)
571.4 μl of water (100 v/v)
G6
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium thiocyanate (2 M)
500.0 μl of water (100 v/v)
G7
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
520.0 μl of water (100 v/v)
G8
  • tri-Potassium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
NA
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Potassium citrate (1.5 M)
466.7 μl of water (100 v/v)
G9
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.2 M -
pH 4.6
100.0 μl of Ammonium sulfate (2 M)
250.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
550.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 4,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.2 M -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H2
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
pH 5
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
500.0 μl of water (100 v/v)
H3
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 10 w/v -
pH 8.5
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H4
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 20 w/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
H5
  • Lithium chloride 1 M -
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 6
pH 6
200.0 μl of Lithium chloride (5 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
300.0 μl of water (100 v/v)
H6
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
pH 5
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
300.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 8
  • Calcium acetate 0.2 M -
pH 8
200.0 μl of Calcium acetate (1 M)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
500.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 8,000 20 w/v -
  • MES 0.1 M pH 6
  • Calcium acetate 0.2 M -
pH 6
200.0 μl of Calcium acetate (1 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
300.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Magnesium acetate 0.2 M -
pH 6.5
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
300.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
420.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 20 w/v -
  • CAPS 0.1 M pH 10
  • Sodium chloride 0.2 M -
pH 10
100.0 μl of CAPS pH 10 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
300.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Sodium acetate 0.2 M -
pH 6.5
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
200.0 μl of water (100 v/v)