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Supplemental Information

Screen Description

JCSG Top96

Rigaku / JCSG Top96
Created 2018-10-02.

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JCSG Top96

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JCSG Top96

    Screen Structure

    JCSG Top96

    Well Count 96
    Number of distinct ingredients 54
    Internal diversity (score mean dist) 0.948

    Screen Composition

    More information about screen composition chart.
    2025-06-15T02:23:14.291414 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type JCSG Top96
    Buffer 100
    Cryoprotectant 12
    Organic (non-volatile) 29
    Organic (volatile) 4
    Polymer 74
    Salt 109

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    JCSG Top96 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Sodium acetate 0.1 M pH 4.5
    • 1,2-Propanediol 40 v/v -
    		pH 4.5
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of 1,2-Propanediol (100 v/v)
    500 μl of water (100 v/v)
    A2
    • Ethylene glycol 25 v/v -
    		NA
    250 μl of Ethylene glycol (100 v/v)
    750 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 0.1 M pH 2.5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 2.5
    75 μl of tri-Sodium citrate pH 3 (1 M)
    24 μl of tri-Sodium citrate pH 1 (1 M)
    333 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
    566 μl of water (100 v/v)
    A4
    • Sodium acetate 0.1 M pH 5
    • 2-methyl-2,4-pentanediol BCC 10 v/v -
    		pH 5
    333 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
    100 μl of Sodium acetate pH 5 (1 M)
    566 μl of water (100 v/v)
    A5
    • HEPES 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    		pH 6.5
    100 μl of HEPES pH 6.5 (1 M)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    500 μl of water (100 v/v)
    A6
    • BICINE 0.1 M pH 8.5
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    		pH 8.5
    100 μl of BICINE pH 8.5 (1 M)
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    A7
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 20 v/v -
    • Ammonium sulfate 0.2 M -
    • Glycerol 10 v/v -
    		pH 4.2
    333 μl of Glycerol (30 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Ammonium sulfate (2 M)
    266 μl of water (100 v/v)
    A8
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 400 1.7 v/v -
    • Ammonium sulfate 1.7 M -
    • Glycerol 15 v/v -
    		pH 7.5
    425 μl of Ammonium sulfate (4 M)
    85 μl of HEPES pH 7.5 (1 M)
    187 μl of Glycerol (80 v/v)
    113 μl of Polyethylene glycol 400 (15 v/v)
    189 μl of water (100 v/v)
    A9
    • Sodium acetate 0.08 M pH 4.6
    • Polyethylene glycol 4,000 20 w/v -
    • Ammonium sulfate 0.16 M -
    • Glycerol 20 v/v -
    		pH 4.6
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    160 μl of Ammonium sulfate (1 M)
    80 μl of Sodium acetate pH 4.6 (1 M)
    200 μl of Glycerol (100 v/v)
    360 μl of water (100 v/v)
    A10
    • tri-Sodium citrate 0.085 M pH 5.6
    • Polyethylene glycol 4,000 25.5 w/v -
    • Ammonium acetate 0.17 M -
    • Glycerol 15 v/v -
    		pH 5.6
    255 μl of Polyethylene glycol 4,000 (100 w/v)
    300 μl of Glycerol (50 v/v)
    170 μl of Ammonium acetate (1 M)
    85 μl of tri-Sodium citrate pH 5.6 (1 M)
    190 μl of water (100 v/v)
    A11
    • tris chloride BCC 0.085 M pH 8.5
    • Polyethylene glycol 4,000 25.5 w/v -
    • Glycerol 15 v/v -
    • Sodium acetate 0.17 M -
    		pH 8.5
    255 μl of Polyethylene glycol 4,000 (100 w/v)
    85 μl of tris chloride BCC pH 8.5 (1 M)
    187 μl of Glycerol (80 v/v)
    85 μl of Sodium acetate (2 M)
    387 μl of water (100 v/v)
    A12
    • Sodium cacodylate 0.08 M pH 6.5
    • Polyethylene glycol 8,000 16 w/v -
    • Glycerol 20 v/v -
    • Magnesium acetate 0.16 M -
    		pH 6.5
    320 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Glycerol (100 v/v)
    160 μl of Magnesium acetate (1 M)
    80 μl of Sodium cacodylate pH 6.5 (1 M)
    240 μl of water (100 v/v)
    B1
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 30 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    • Glycerol 10 v/v -
    		pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    125 μl of Glycerol (80 v/v)
    375 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 30 w/v -
    • Polyethylene glycol 1,000 5 w/v -
    • Glycerol 10 v/v -
    		pH 6
    400 μl of Polyethylene glycol 600 (75 w/v)
    200 μl of Glycerol (50 v/v)
    100 μl of MES pH 6 (1 M)
    100 μl of Polyethylene glycol 1,000 (50 w/v)
    200 μl of water (100 v/v)
    B3
    • Sodium acetate 0.07 M pH 4.6
    • Polyethylene glycol 4,000 5.6 w/v -
    • Glycerol 30 v/v -
    		pH 4.6
    112 μl of Polyethylene glycol 4,000 (50 w/v)
    375 μl of Glycerol (80 v/v)
    70 μl of Sodium acetate pH 4.6 (1 M)
    443 μl of water (100 v/v)
    B4
    • HEPES 0.085 M pH 7.5
    • Polyethylene glycol 4,000 17 w/v -
    • 2-Propanol 8.5 v/v -
    • Glycerol 15 v/v -
    		pH 7.5
    283 μl of 2-Propanol (30 v/v)
    187 μl of Glycerol (80 v/v)
    85 μl of HEPES pH 7.5 (1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    104 μl of water (100 v/v)
    B5
    • tri-Sodium citrate 0.095 M pH 5.6
    • Polyethylene glycol 4,000 19 w/v -
    • 2-Propanol 19 v/v -
    • Glycerol 5 v/v -
    		pH 5.6
    100 μl of Glycerol (50 v/v)
    95 μl of tri-Sodium citrate pH 5.6 (1 M)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    190 μl of 2-Propanol (100 v/v)
    235 μl of water (100 v/v)
    B6
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 10 v/v -
    		pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of 2-Propanol (100 v/v)
    400 μl of water (100 v/v)
    B7
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 20 w/v -
    • 2-Propanol 20 v/v -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of 2-Propanol (100 v/v)
    300 μl of water (100 v/v)
    B8
    • Sodium cacodylate 0.1 M pH 6.5
    • 2-methyl-2,4-pentanediol BCC 40 v/v -
    • Polyethylene glycol 8,000 5 w/v -
    		pH 6.5
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of water (100 v/v)
    B9
    • Sodium acetate 0.08 M pH 4.6
    • Ammonium sulfate 1.6 M -
    • Glycerol 20 v/v -
    		pH 4.6
    80 μl of Sodium acetate pH 4.6 (1 M)
    444 μl of Ammonium sulfate (3.6 M)
    200 μl of Glycerol (100 v/v)
    275 μl of water (100 v/v)
    B10
    • Sodium acetate 0.1 M pH 4.6
    • 2-methyl-2,4-pentanediol BCC 30 v/v -
    • Sodium chloride 0.2 M -
    		pH 4.6
    300 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Sodium chloride (2 M)
    500 μl of water (100 v/v)
    B11
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.2 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    66 μl of Ammonium acetate (3 M)
    833 μl of water (100 v/v)
    B12
    • MES 0.1 M pH 6
    • 2-methyl-2,4-pentanediol BCC 35 v/v -
    • Lithium sulfate 0.2 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    350 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    100 μl of Lithium sulfate (2 M)
    450 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 200 50 v/v -
    		pH 7
    500 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    400 μl of water (100 v/v)
    C2
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 40 v/v -
    • Polyethylene glycol 3,000 5 w/v -
    		pH 6
    100 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    C3
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 600 40 w/v -
    		pH 9.5
    533 μl of Polyethylene glycol 600 (75 w/v)
    100 μl of CHES pH 9.5 (1 M)
    366 μl of water (100 v/v)
    C4
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 3,000 20 w/v -
    		pH 5.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    500 μl of water (100 v/v)
    C5
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 5 w/v -
    		pH 4
    100 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    800 μl of water (100 v/v)
    C6
    • tri-Sodium citrate 0.1 M pH 4
    • Polyethylene glycol 6,000 20 w/v -
    		pH 4
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    500 μl of water (100 v/v)
    C7
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 8.5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    500 μl of water (100 v/v)
    C8
    • MES 0.1 M pH 5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 5
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 5 (1 M)
    300 μl of water (100 v/v)
    C9
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 6,000 30 w/v -
    		pH 8.5
    600 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    300 μl of water (100 v/v)
    C10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 7.5
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    500 μl of water (100 v/v)
    C11
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 20 w/v -
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 0.1 M pH 3.5
    • Ammonium sulfate 0.8 M -
    		pH 3.5
    266 μl of Ammonium sulfate (3 M)
    100 μl of tri-Sodium citrate pH 3.5 (1 M)
    633 μl of water (100 v/v)
    D1
    • tri-Sodium citrate 0.1 M pH 4
    • Ammonium sulfate 1.6 M -
    		pH 4
    457 μl of Ammonium sulfate (3.5 M)
    100 μl of tri-Sodium citrate pH 4 (1 M)
    442 μl of water (100 v/v)
    D2
    • MES 0.1 M pH 5
    • Ammonium sulfate 1.6 M -
    		pH 5
    100 μl of MES pH 5 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    D3
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 1.6 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    457 μl of Ammonium sulfate (3.5 M)
    442 μl of water (100 v/v)
    D4
    • Sodium cacodylate 0.1 M pH 6.5
    • Ammonium sulfate 2 M -
    • Sodium chloride 0.2 M -
    		pH 6.5
    40 μl of Sodium chloride (5 M)
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    288 μl of water (100 v/v)
    D5
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    328 μl of water (100 v/v)
    D6
    • CAPS 0.1 M pH 10
    • Ammonium sulfate 2 M -
    • Lithium sulfate 0.2 M -
    		pH 10
    100 μl of CAPS pH 10 (1 M)
    571 μl of Ammonium sulfate (3.5 M)
    66 μl of Lithium sulfate (3 M)
    261 μl of water (100 v/v)
    D7
    • tris chloride BCC 0.1 M pH 8.5
    • Ammonium sulfate 2.4 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    D8
    • BICINE 0.1 M pH 9
    • Ammonium sulfate 2.4 M -
    		pH 9
    100 μl of BICINE pH 9 (1 M)
    685 μl of Ammonium sulfate (3.5 M)
    214 μl of water (100 v/v)
    D9
    • Sodium acetate 0.1 M pH 4.5
    • Lithium sulfate 0.2 M -
    • Sodium chloride 2.5 M -
    		pH 4.5
    500 μl of Sodium chloride (5 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    300 μl of water (100 v/v)
    D10
    • Sodium cacodylate 0.1 M pH 6.5
    • tri-Sodium citrate 1 M -
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    D11
    • tris chloride BCC 0.1 M pH 7
    • Sodium chloride 0.2 M -
    • tri-Sodium citrate 1 M -
    		pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    500 μl of tri-Sodium citrate pH 6.5 (2 M)
    100 μl of Sodium chloride (2 M)
    300 μl of water (100 v/v)
    D12
    • CHES 0.1 M pH 9.5
    • tri-Sodium citrate 1 M -
    		pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    625 μl of tri-Sodium citrate (1.6 M)
    275 μl of water (100 v/v)
    E1
    • HEPES 0.1 M pH 7.5
    • tri-Sodium citrate 1.4 M -
    		pH 7.5
    466 μl of tri-Sodium citrate (3 M)
    100 μl of HEPES pH 7.5 (1 M)
    433 μl of water (100 v/v)
    E2
    • CAPS 0.1 M pH 10
    • Lithium sulfate 0.2 M -
    • di-Potassium hydrogen phosphate 0.8 M -
    • Sodium Phosphate Monobasic 1.2 M -
    		pH 10
    266 μl of di-Potassium hydrogen phosphate (3 M)
    400 μl of Sodium Phosphate Monobasic (3 M)
    100 μl of Lithium sulfate (2 M)
    100 μl of CAPS pH 10 (1 M)
    133 μl of water (100 v/v)
    E3
    • Sodium phosphate citrate 0.1 M pH 4.2
    • Polyethylene glycol 200 50 v/v -
    • Sodium chloride 0.2 M -
    		pH 4.2
    500 μl of Polyethylene glycol 200 (100 v/v)
    100 μl of Sodium phosphate citrate pH 4.2 (1 M)
    40 μl of Sodium chloride (5 M)
    360 μl of water (100 v/v)
    E4
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 300 40 v/v -
    • Calcium acetate 0.2 M -
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    400 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Calcium acetate (2 M)
    400 μl of water (100 v/v)
    E5
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 28 v/v -
    • Calcium chloride 0.2 M -
    		pH 7.5
    100 μl of Calcium chloride (2 M)
    100 μl of HEPES pH 7.5 (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    E6
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 30 v/v -
    • Calcium acetate 0.2 M -
    		pH 4.5
    200 μl of Calcium acetate (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    E7
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 40 v/v -
    • Lithium sulfate 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    E8
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    • Magnesium chloride 0.2 M -
    		pH 5.5
    100 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 5.5 (1 M)
    400 μl of water (100 v/v)
    E9
    • Sodium cacodylate 0.1 M pH 6.5
    • Polyethylene glycol 3,000 10 w/v -
    • Magnesium chloride 0.2 M -
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    600 μl of water (100 v/v)
    E10
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,000 20 w/v -
    • Sodium chloride 0.2 M -
    		pH 7.5
    400 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of HEPES pH 7.5 (1 M)
    100 μl of Sodium chloride (2 M)
    400 μl of water (100 v/v)
    E11
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,000 30 w/v -
    • Sodium chloride 0.2 M -
    		pH 7
    600 μl of Polyethylene glycol 3,000 (50 w/v)
    100 μl of tris chloride BCC pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    260 μl of water (100 v/v)
    E12
    • Ammonium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    20 μl of Ammonium fluoride (10 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    580 μl of water (100 v/v)
    F1
    • Ammonium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of Ammonium formate (2.5 M)
    520 μl of water (100 v/v)
    F2
    • Ammonium sulfate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Ammonium sulfate (1 M)
    400 μl of water (100 v/v)
    F3
    • Calcium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    400 μl of water (100 v/v)
    F4
    • Calcium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Calcium chloride (2 M)
    500 μl of water (100 v/v)
    F5
    • di-Ammonium hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    57 μl of di-Ammonium hydrogen phosphate (3.5 M)
    542 μl of water (100 v/v)
    F6
    • di-Ammonium tartrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Ammonium tartrate (2 M)
    500 μl of water (100 v/v)
    F7
    • di-Sodium hydrogen phosphate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of di-Sodium hydrogen phosphate (2 M)
    500 μl of water (100 v/v)
    F8
    • Magnesium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    100 μl of Magnesium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    F9
    • Magnesium chloride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    100 μl of Magnesium chloride (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    F10
    • Magnesium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium formate (1 M)
    400 μl of water (100 v/v)
    F11
    • Magnesium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    200 μl of Magnesium nitrate (1 M)
    400 μl of water (100 v/v)
    F12
    • Potassium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Potassium formate (2 M)
    500 μl of water (100 v/v)
    G1
    • Potassium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    100 μl of Potassium nitrate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    G2
    • Sodium acetate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    100 μl of Sodium acetate (2 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    500 μl of water (100 v/v)
    G3
    • Sodium fluoride 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium fluoride (2 M)
    500 μl of water (100 v/v)
    G4
    • Sodium formate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of Sodium formate (4 M)
    550 μl of water (100 v/v)
    G5
    • Sodium nitrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    28 μl of Sodium nitrate (7 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    571 μl of water (100 v/v)
    G6
    • Sodium thiocyanate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Sodium thiocyanate (2 M)
    500 μl of water (100 v/v)
    G7
    • tri-Ammonium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    80 μl of tri-Ammonium citrate (2.5 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    520 μl of water (100 v/v)
    G8
    • tri-Potassium citrate 0.2 M -
    • Polyethylene glycol 3,350 20 w/v -
    		NA
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    133 μl of tri-Potassium citrate (1.5 M)
    466 μl of water (100 v/v)
    G9
    • Polyethylene glycol 4,000 25 w/v -
    • Sodium acetate 0.1 M pH 4.6
    • Ammonium sulfate 0.2 M -
    		pH 4.6
    250 μl of Polyethylene glycol 4,000 (100 w/v)
    100 μl of Sodium acetate pH 4.6 (1 M)
    100 μl of Ammonium sulfate (2 M)
    550 μl of water (100 v/v)
    G10
    • Polyethylene glycol 4,000 30 w/v -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Ammonium acetate 0.2 M -
    		pH 5.6
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    300 μl of Polyethylene glycol 4,000 (100 w/v)
    200 μl of Ammonium acetate (1 M)
    400 μl of water (100 v/v)
    G11
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Lithium sulfate 0.2 M -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (100 w/v)
    100 μl of Lithium sulfate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G12
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (100 w/v)
    80 μl of Magnesium chloride (2.5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    520 μl of water (100 v/v)
    H1
    • Polyethylene glycol 4,000 30 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Sodium acetate 0.2 M -
    		pH 8.5
    300 μl of Polyethylene glycol 4,000 (100 w/v)
    100 μl of Sodium acetate (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    H2
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Lithium chloride (5 M)
    500 μl of water (100 v/v)
    H3
    • Lithium chloride 1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 6,000 10 w/v -
    		pH 8.5
    200 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Lithium chloride (5 M)
    500 μl of water (100 v/v)
    H4
    • Lithium chloride 1 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 6,000 20 w/v -
    		pH 7
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    200 μl of Lithium chloride (5 M)
    300 μl of water (100 v/v)
    H5
    • Lithium chloride 1 M -
    • Polyethylene glycol 6,000 20 w/v -
    • MES 0.1 M pH 6
    		pH 6
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of MES pH 6 (1 M)
    200 μl of Lithium chloride (5 M)
    300 μl of water (100 v/v)
    H6
    • Lithium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 6,000 20 w/v -
    		pH 5
    400 μl of Polyethylene glycol 6,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    200 μl of Lithium chloride (5 M)
    300 μl of water (100 v/v)
    H7
    • Polyethylene glycol 8,000 10 w/v -
    • Imidazole 0.1 M pH 8
    • Calcium acetate 0.2 M -
    		pH 8
    100 μl of Imidazole pH 8 (1 M)
    200 μl of Calcium acetate (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of water (100 v/v)
    H8
    • Polyethylene glycol 8,000 20 w/v -
    • MES 0.1 M pH 6
    • Calcium acetate 0.2 M -
    		pH 6
    100 μl of MES pH 6 (1 M)
    200 μl of Calcium acetate (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    300 μl of water (100 v/v)
    H9
    • Polyethylene glycol 8,000 20 w/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Magnesium acetate 0.2 M -
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    200 μl of Magnesium acetate (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    300 μl of water (100 v/v)
    H10
    • Polyethylene glycol 8,000 20 w/v -
    • tris chloride BCC 0.1 M pH 8.5
    • Magnesium chloride 0.2 M -
    		pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (2.5 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    420 μl of water (100 v/v)
    H11
    • Polyethylene glycol 8,000 20 w/v -
    • CAPS 0.1 M pH 10
    • Sodium chloride 0.2 M -
    		pH 10
    100 μl of CAPS pH 10 (1 M)
    400 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Sodium chloride (1 M)
    300 μl of water (100 v/v)
    H12
    • Polyethylene glycol 8,000 30 w/v -
    • Sodium cacodylate 0.1 M pH 6.5
    • Sodium acetate 0.2 M -
    		pH 6.5
    100 μl of Sodium cacodylate pH 6.5 (1 M)
    100 μl of Sodium acetate (2 M)
    600 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of water (100 v/v)