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Supplemental Information

Screen Description

JCSG Top96 CRYO

Rigaku / JCSG Top96 CRYO
Created 2019-01-17.

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Barcodes

JCSG Top96 CRYO

No barcodes found.

Screen Structure

JCSG Top96 CRYO

Well Count 96
Number of distinct ingredients 54
Internal diversity (score mean dist) 0.910

Screen Composition

2025-08-16T06:25:42.564371 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type JCSG Top96 CRYO
Buffer 100
Cryoprotectant 25
Organic (non-volatile) 87
Organic (volatile) 4
Polymer 80
Salt 109

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
JCSG Top96 CRYO Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.1 M pH 4.5
  • 1,2-Propanediol 40 v/v -
pH 4.5
400.0 μl of 1,2-Propanediol (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of water (100 v/v)
A2
  • Ethylene glycol 35 v/v -
NA
350.0 μl of Ethylene glycol (100 v/v)
650.0 μl of water (100 v/v)
A3
  • tri-Sodium citrate 0.1 M pH 2.5
  • 2-methyl-2,4-pentanediol BCC 25 v/v -
pH 2.5
250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
70.8 μl of tri-Sodium citrate pH 1 (1 M)
29.2 μl of tri-Sodium citrate pH 3 (1 M)
650.0 μl of water (100 v/v)
A4
  • Sodium acetate 0.1 M pH 5
  • 2-methyl-2,4-pentanediol BCC 20 v/v -
pH 5
666.7 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
233.3 μl of water (100 v/v)
A5
  • HEPES 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of HEPES pH 6.5 (1 M)
500.0 μl of water (100 v/v)
A6
  • BICINE 0.1 M pH 8.5
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
pH 8.5
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of BICINE pH 8.5 (1 M)
250.0 μl of water (100 v/v)
A7
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 300 20 v/v -
  • Ammonium sulfate 0.2 M -
  • Glycerol 10 v/v -
pH 4.2
100.0 μl of Ammonium sulfate (2 M)
333.3 μl of Glycerol (30 v/v)
200.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
266.7 μl of water (100 v/v)
A8
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 400 1.7 v/v -
  • Ammonium sulfate 1.7 M -
  • Glycerol 15 v/v -
pH 7.5
425.0 μl of Ammonium sulfate (4 M)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
113.3 μl of Polyethylene glycol 400 (15 v/v)
189.2 μl of water (100 v/v)
A9
  • Sodium acetate 0.08 M pH 4.6
  • Polyethylene glycol 4,000 20 w/v -
  • Ammonium sulfate 0.16 M -
  • Glycerol 20 v/v -
pH 4.6
160.0 μl of Ammonium sulfate (1 M)
200.0 μl of Glycerol (100 v/v)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
360.0 μl of water (100 v/v)
A10
  • tri-Sodium citrate 0.085 M pH 5.6
  • Polyethylene glycol 4,000 25.5 w/v -
  • Ammonium acetate 0.17 M -
  • Glycerol 15 v/v -
pH 5.6
170.0 μl of Ammonium acetate (1 M)
300.0 μl of Glycerol (50 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of tri-Sodium citrate pH 5.6 (1 M)
190.0 μl of water (100 v/v)
A11
  • tris chloride BCC 0.085 M pH 8.5
  • Polyethylene glycol 4,000 25.5 w/v -
  • Glycerol 15 v/v -
  • Sodium acetate 0.17 M -
pH 8.5
187.5 μl of Glycerol (80 v/v)
255.0 μl of Polyethylene glycol 4,000 (100 w/v)
85.0 μl of Sodium acetate (2 M)
85.0 μl of tris chloride BCC pH 8.5 (1 M)
387.5 μl of water (100 v/v)
A12
  • Sodium cacodylate 0.08 M pH 6.5
  • Polyethylene glycol 8,000 16 w/v -
  • Glycerol 20 v/v -
  • Magnesium acetate 0.16 M -
pH 6.5
200.0 μl of Glycerol (100 v/v)
160.0 μl of Magnesium acetate (1 M)
320.0 μl of Polyethylene glycol 8,000 (50 w/v)
80.0 μl of Sodium cacodylate pH 6.5 (1 M)
240.0 μl of water (100 v/v)
B1
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 30 v/v -
  • Polyethylene glycol 3,000 5 w/v -
  • Glycerol 10 v/v -
pH 7.5
125.0 μl of Glycerol (80 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
300.0 μl of Polyethylene glycol 400 (100 v/v)
375.0 μl of water (100 v/v)
B2
  • MES 0.1 M pH 6
  • Polyethylene glycol 600 30 w/v -
  • Polyethylene glycol 1,000 5 w/v -
  • Glycerol 10 v/v -
pH 6
200.0 μl of Glycerol (50 v/v)
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 1,000 (50 w/v)
400.0 μl of Polyethylene glycol 600 (75 w/v)
200.0 μl of water (100 v/v)
B3
  • Sodium acetate 0.07 M pH 4.6
  • Polyethylene glycol 4,000 5.6 w/v -
  • Glycerol 30 v/v -
pH 4.6
375.0 μl of Glycerol (80 v/v)
112.0 μl of Polyethylene glycol 4,000 (50 w/v)
70.0 μl of Sodium acetate pH 4.6 (1 M)
443.0 μl of water (100 v/v)
B4
  • HEPES 0.085 M pH 7.5
  • Polyethylene glycol 4,000 17 w/v -
  • 2-Propanol 8.5 v/v -
  • Glycerol 15 v/v -
  • Ethylene glycol 10 v/v -
pH 7.5
283.3 μl of 2-Propanol (30 v/v)
100.0 μl of Ethylene glycol (100 v/v)
187.5 μl of Glycerol (80 v/v)
85.0 μl of HEPES pH 7.5 (1 M)
340.0 μl of Polyethylene glycol 4,000 (50 w/v)
4.2 μl of water (100 v/v)
B5
  • tri-Sodium citrate 0.095 M pH 5.6
  • Polyethylene glycol 4,000 19 w/v -
  • 2-Propanol 19 v/v -
  • Glycerol 5 v/v -
pH 5.6
190.0 μl of 2-Propanol (100 v/v)
100.0 μl of Glycerol (50 v/v)
380.0 μl of Polyethylene glycol 4,000 (50 w/v)
95.0 μl of tri-Sodium citrate pH 5.6 (1 M)
235.0 μl of water (100 v/v)
B6
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 10 v/v -
  • Glycerol 10 v/v -
pH 7.5
100.0 μl of 2-Propanol (100 v/v)
100.0 μl of Glycerol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
300.0 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.1 M pH 5.6
  • Polyethylene glycol 4,000 20 w/v -
  • 2-Propanol 20 v/v -
  • Ethylene glycol 10 v/v -
pH 5.6
200.0 μl of 2-Propanol (100 v/v)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
200.0 μl of water (100 v/v)
B8
  • Sodium cacodylate 0.1 M pH 6.5
  • 2-methyl-2,4-pentanediol BCC 40 v/v -
  • Polyethylene glycol 8,000 5 w/v -
pH 6.5
400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
B9
  • Sodium acetate 0.08 M pH 4.6
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
pH 4.6
444.4 μl of Ammonium sulfate (3.6 M)
200.0 μl of Glycerol (100 v/v)
80.0 μl of Sodium acetate pH 4.6 (1 M)
275.6 μl of water (100 v/v)
B10
  • Sodium acetate 0.1 M pH 4.6
  • 2-methyl-2,4-pentanediol BCC 30 v/v -
  • Sodium chloride 0.2 M -
pH 4.6
300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
100.0 μl of Sodium chloride (2 M)
500.0 μl of water (100 v/v)
B11
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
pH 5.6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
66.7 μl of Ammonium acetate (3 M)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
483.3 μl of water (100 v/v)
B12
  • MES 0.1 M pH 6
  • 2-methyl-2,4-pentanediol BCC 35 v/v -
  • Lithium sulfate 0.2 M -
pH 6
350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of MES pH 6 (1 M)
450.0 μl of water (100 v/v)
C1
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 200 50 v/v -
pH 7
500.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
400.0 μl of water (100 v/v)
C2
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 40 v/v -
  • Polyethylene glycol 3,000 5 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
100.0 μl of Polyethylene glycol 3,000 (50 w/v)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
C3
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 600 40 w/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
533.3 μl of Polyethylene glycol 600 (75 w/v)
366.7 μl of water (100 v/v)
C4
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 3,000 20 w/v -
  • Ethylene glycol 10 v/v -
pH 5.5
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
400.0 μl of water (100 v/v)
C5
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 5 w/v -
  • Polyethylene glycol 200 25 v/v -
pH 4
250.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
550.0 μl of water (100 v/v)
C6
  • tri-Sodium citrate 0.1 M pH 4
  • Polyethylene glycol 6,000 20 w/v -
  • Glycerol 15 v/v -
pH 4
150.0 μl of Glycerol (100 v/v)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
350.0 μl of water (100 v/v)
C7
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 20 w/v -
  • Ethylene glycol 10 v/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
400.0 μl of water (100 v/v)
C8
  • MES 0.1 M pH 5
  • Polyethylene glycol 6,000 30 w/v -
  • Polyethylene glycol 200 10 v/v -
pH 5
100.0 μl of MES pH 5 (1 M)
100.0 μl of Polyethylene glycol 200 (100 v/v)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
200.0 μl of water (100 v/v)
C9
  • BICINE 0.1 M pH 8.5
  • Polyethylene glycol 6,000 30 w/v -
  • Ethylene glycol 5 v/v -
pH 8.5
100.0 μl of BICINE pH 8.5 (1 M)
50.0 μl of Ethylene glycol (100 v/v)
600.0 μl of Polyethylene glycol 6,000 (50 w/v)
250.0 μl of water (100 v/v)
C10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 8,000 20 w/v -
  • Ethylene glycol 10 v/v -
pH 7.5
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
400.0 μl of water (100 v/v)
C11
  • CHES 0.1 M pH 9.5
  • Polyethylene glycol 8,000 20 w/v -
  • Polyethylene glycol 200 12 v/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
120.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
380.0 μl of water (100 v/v)
C12
  • tri-Sodium citrate 0.1 M pH 3.5
  • Ammonium sulfate 0.8 M -
  • Glycerol 20 v/v -
pH 3.5
266.7 μl of Ammonium sulfate (3 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of tri-Sodium citrate pH 3.5 (1 M)
433.3 μl of water (100 v/v)
D1
  • tri-Sodium citrate 0.1 M pH 4
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
pH 4
457.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of tri-Sodium citrate pH 4 (1 M)
242.9 μl of water (100 v/v)
D2
  • MES 0.1 M pH 5
  • Ammonium sulfate 1.6 M -
  • Glycerol 20 v/v -
pH 5
457.1 μl of Ammonium sulfate (3.5 M)
200.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 5 (1 M)
242.9 μl of water (100 v/v)
D3
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 1.6 M -
  • Glycerol 15 v/v -
pH 9
457.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of BICINE pH 9 (1 M)
150.0 μl of Glycerol (100 v/v)
292.9 μl of water (100 v/v)
D4
  • Sodium cacodylate 0.1 M pH 6.5
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
  • Ethylene glycol 15 v/v -
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
150.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
138.6 μl of water (100 v/v)
D5
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2 M -
  • Ethylene glycol 15 v/v -
pH 8.5
571.4 μl of Ammonium sulfate (3.5 M)
150.0 μl of Ethylene glycol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
178.6 μl of water (100 v/v)
D6
  • CAPS 0.1 M pH 10
  • Ammonium sulfate 2 M -
  • Lithium sulfate 0.2 M -
  • Ethylene glycol 15 v/v -
pH 10
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of CAPS pH 10 (1 M)
150.0 μl of Ethylene glycol (100 v/v)
66.7 μl of Lithium sulfate (3 M)
111.9 μl of water (100 v/v)
D7
  • tris chloride BCC 0.1 M pH 8.5
  • Ammonium sulfate 2.4 M -
  • Glycerol 15 v/v -
pH 8.5
685.7 μl of Ammonium sulfate (3.5 M)
150.0 μl of Glycerol (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
64.3 μl of water (100 v/v)
D8
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2.4 M -
  • Glycerol 20 v/v -
pH 9
480.0 μl of Ammonium sulfate (5 M)
100.0 μl of BICINE pH 9 (1 M)
200.0 μl of Glycerol (100 v/v)
220.0 μl of water (100 v/v)
D9
  • Sodium acetate 0.1 M pH 4.5
  • Lithium sulfate 0.2 M -
  • Sodium chloride 2.5 M -
  • Ethylene glycol 10 v/v -
pH 4.5
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Lithium sulfate (2 M)
100.0 μl of Sodium acetate pH 4.5 (1 M)
500.0 μl of Sodium chloride (5 M)
200.0 μl of water (100 v/v)
D10
  • Sodium cacodylate 0.1 M pH 6.5
  • tri-Sodium citrate 1 M -
  • Glycerol 15 v/v -
pH 6.5
150.0 μl of Glycerol (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
625.0 μl of tri-Sodium citrate (1.6 M)
125.0 μl of water (100 v/v)
D11
  • tris chloride BCC 0.1 M pH 7
  • Sodium chloride 0.2 M -
  • tri-Sodium citrate 1 M -
  • Ethylene glycol 10 v/v -
pH 7
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Sodium chloride (2 M)
500.0 μl of tri-Sodium citrate pH 6.5 (2 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
200.0 μl of water (100 v/v)
D12
  • CHES 0.1 M pH 9.5
  • tri-Sodium citrate 1 M -
  • Ethylene glycol 10 v/v -
pH 9.5
100.0 μl of CHES pH 9.5 (1 M)
100.0 μl of Ethylene glycol (100 v/v)
625.0 μl of tri-Sodium citrate (1.6 M)
175.0 μl of water (100 v/v)
E1
  • HEPES 0.1 M pH 7.5
  • tri-Sodium citrate 1.4 M -
  • Ethylene glycol 10 v/v -
pH 7.5
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
466.7 μl of tri-Sodium citrate (3 M)
333.3 μl of water (100 v/v)
E2
  • CAPS 0.1 M pH 10
  • Lithium sulfate 0.2 M -
  • di-Potassium hydrogen phosphate 0.8 M -
  • Sodium Phosphate Monobasic 1.2 M -
  • Glycerol 25 v/v -
pH 10
100.0 μl of CAPS pH 10 (1 M)
250.0 μl of Glycerol (100 v/v)
40.0 μl of Lithium sulfate (5 M)
400.0 μl of Sodium Phosphate Monobasic (3 M)
160.0 μl of di-Potassium hydrogen phosphate (5 M)
50.0 μl of water (100 v/v)
E3
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 200 50 v/v -
  • Sodium chloride 0.2 M -
pH 4.2
500.0 μl of Polyethylene glycol 200 (100 v/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
360.0 μl of water (100 v/v)
E4
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 300 40 v/v -
  • Calcium acetate 0.2 M -
pH 6.5
100.0 μl of Calcium acetate (2 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
400.0 μl of water (100 v/v)
E5
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 28 v/v -
  • Calcium chloride 0.2 M -
  • Ethylene glycol 10 v/v -
pH 7.5
100.0 μl of Calcium chloride (2 M)
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
420.0 μl of water (100 v/v)
E6
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
  • Calcium acetate 0.2 M -
pH 4.5
200.0 μl of Calcium acetate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
400.0 μl of water (100 v/v)
E7
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 45 v/v -
  • Lithium sulfate 0.2 M -
pH 8.5
100.0 μl of Lithium sulfate (2 M)
450.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
E8
  • MES 0.1 M pH 5.5
  • Polyethylene glycol 400 40 v/v -
  • Magnesium chloride 0.2 M -
pH 5.5
100.0 μl of MES pH 5.5 (1 M)
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
E9
  • Sodium cacodylate 0.1 M pH 6.5
  • Polyethylene glycol 3,000 10 w/v -
  • Magnesium chloride 0.2 M -
  • Ethylene glycol 15 v/v -
pH 6.5
150.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
200.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
450.0 μl of water (100 v/v)
E10
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 3,000 20 w/v -
  • Sodium chloride 0.2 M -
  • Ethylene glycol 10 v/v -
pH 7.5
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7.5 (1 M)
400.0 μl of Polyethylene glycol 3,000 (50 w/v)
100.0 μl of Sodium chloride (2 M)
300.0 μl of water (100 v/v)
E11
  • tris chloride BCC 0.1 M pH 7
  • Polyethylene glycol 3,000 30 w/v -
  • Sodium chloride 0.2 M -
pH 7
600.0 μl of Polyethylene glycol 3,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E12
  • Ammonium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 15 v/v -
NA
20.0 μl of Ammonium fluoride (10 M)
150.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
430.0 μl of water (100 v/v)
F1
  • Ammonium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
80.0 μl of Ammonium formate (2.5 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
420.0 μl of water (100 v/v)
F2
  • Ammonium sulfate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
200.0 μl of Ammonium sulfate (1 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
F3
  • Calcium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
200.0 μl of Calcium acetate (1 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
F4
  • Calcium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Calcium chloride (2 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F5
  • di-Ammonium hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
442.9 μl of water (100 v/v)
F6
  • di-Ammonium tartrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 15 v/v -
NA
150.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Ammonium tartrate (2 M)
350.0 μl of water (100 v/v)
F7
  • di-Sodium hydrogen phosphate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 15 v/v -
NA
150.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of di-Sodium hydrogen phosphate (2 M)
350.0 μl of water (100 v/v)
F8
  • Magnesium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Magnesium acetate (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F9
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Magnesium chloride (2 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
F10
  • Magnesium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Magnesium formate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
300.0 μl of water (100 v/v)
F11
  • Magnesium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 15 v/v -
NA
150.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Magnesium nitrate (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
250.0 μl of water (100 v/v)
F12
  • Potassium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium formate (2 M)
400.0 μl of water (100 v/v)
G1
  • Potassium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Potassium nitrate (2 M)
400.0 μl of water (100 v/v)
G2
  • Sodium acetate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Polyethylene glycol 200 10 v/v -
NA
100.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate (2 M)
400.0 μl of water (100 v/v)
G3
  • Sodium fluoride 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Polyethylene glycol 200 15 v/v -
NA
150.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium fluoride (2 M)
350.0 μl of water (100 v/v)
G4
  • Sodium formate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Sodium formate (4 M)
450.0 μl of water (100 v/v)
G5
  • Sodium nitrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
28.6 μl of Sodium nitrate (7 M)
471.4 μl of water (100 v/v)
G6
  • Sodium thiocyanate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium thiocyanate (2 M)
400.0 μl of water (100 v/v)
G7
  • tri-Ammonium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
80.0 μl of tri-Ammonium citrate (2.5 M)
420.0 μl of water (100 v/v)
G8
  • tri-Potassium citrate 0.2 M -
  • Polyethylene glycol 3,350 20 w/v -
  • Ethylene glycol 10 v/v -
NA
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
133.3 μl of tri-Potassium citrate (1.5 M)
366.7 μl of water (100 v/v)
G9
  • Polyethylene glycol 4,000 25 w/v -
  • Sodium acetate 0.1 M pH 4.6
  • Ammonium sulfate 0.2 M -
  • Ethylene glycol 10 v/v -
pH 4.6
100.0 μl of Ammonium sulfate (2 M)
100.0 μl of Ethylene glycol (100 v/v)
250.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate pH 4.6 (1 M)
450.0 μl of water (100 v/v)
G10
  • Polyethylene glycol 4,000 30 w/v -
  • tri-Sodium citrate 0.1 M pH 5.6
  • Ammonium acetate 0.2 M -
pH 5.6
200.0 μl of Ammonium acetate (1 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tri-Sodium citrate pH 5.6 (1 M)
400.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Lithium sulfate 0.2 M -
  • Ethylene glycol 10 v/v -
pH 8.5
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
400.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
520.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 4,000 30 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Sodium acetate 0.2 M -
pH 8.5
300.0 μl of Polyethylene glycol 4,000 (100 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
500.0 μl of water (100 v/v)
H2
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 10 w/v -
  • Ethylene glycol 15 v/v -
pH 5
150.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
350.0 μl of water (100 v/v)
H3
  • Lithium chloride 1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 6,000 10 w/v -
  • Ethylene glycol 15 v/v -
pH 8.5
150.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Lithium chloride (5 M)
200.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
H4
  • Lithium chloride 1 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 6,000 20 w/v -
  • Ethylene glycol 10 v/v -
pH 7
100.0 μl of Ethylene glycol (100 v/v)
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
200.0 μl of water (100 v/v)
H5
  • Lithium chloride 1 M -
  • Polyethylene glycol 6,000 20 w/v -
  • MES 0.1 M pH 6
  • Glycerol 20 v/v -
pH 6
200.0 μl of Glycerol (100 v/v)
200.0 μl of Lithium chloride (5 M)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of water (100 v/v)
H6
  • Lithium chloride 1 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 6,000 20 w/v -
  • Glycerol 20 v/v -
pH 5
200.0 μl of Glycerol (100 v/v)
200.0 μl of Lithium chloride (5 M)
400.0 μl of Polyethylene glycol 6,000 (50 w/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
100.0 μl of water (100 v/v)
H7
  • Polyethylene glycol 8,000 10 w/v -
  • Imidazole 0.1 M pH 8
  • Calcium acetate 0.2 M -
  • Ethylene glycol 20 v/v -
pH 8
200.0 μl of Calcium acetate (1 M)
200.0 μl of Ethylene glycol (100 v/v)
100.0 μl of Imidazole pH 8 (1 M)
200.0 μl of Polyethylene glycol 8,000 (50 w/v)
300.0 μl of water (100 v/v)
H8
  • Polyethylene glycol 8,000 20 w/v -
  • MES 0.1 M pH 6
  • Calcium acetate 0.2 M -
  • Glycerol 15 v/v -
pH 6
200.0 μl of Calcium acetate (1 M)
150.0 μl of Glycerol (100 v/v)
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
150.0 μl of water (100 v/v)
H9
  • Polyethylene glycol 8,000 20 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Magnesium acetate 0.2 M -
  • Ethylene glycol 15 v/v -
pH 6.5
150.0 μl of Ethylene glycol (100 v/v)
200.0 μl of Magnesium acetate (1 M)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
150.0 μl of water (100 v/v)
H10
  • Polyethylene glycol 8,000 20 w/v -
  • tris chloride BCC 0.1 M pH 8.5
  • Magnesium chloride 0.2 M -
  • Polyethylene glycol 200 10 v/v -
pH 8.5
80.0 μl of Magnesium chloride (2.5 M)
100.0 μl of Polyethylene glycol 200 (100 v/v)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
320.0 μl of water (100 v/v)
H11
  • Polyethylene glycol 8,000 20 w/v -
  • CAPS 0.1 M pH 10
  • Sodium chloride 0.2 M -
  • Ethylene glycol 10 v/v -
pH 10
100.0 μl of CAPS pH 10 (1 M)
100.0 μl of Ethylene glycol (100 v/v)
400.0 μl of Polyethylene glycol 8,000 (50 w/v)
200.0 μl of Sodium chloride (1 M)
200.0 μl of water (100 v/v)
H12
  • Polyethylene glycol 8,000 30 w/v -
  • Sodium cacodylate 0.1 M pH 6.5
  • Sodium acetate 0.2 M -
  • Ethylene glycol 15 v/v -
pH 6.5
150.0 μl of Ethylene glycol (100 v/v)
600.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of Sodium acetate (2 M)
100.0 μl of Sodium cacodylate pH 6.5 (1 M)
50.0 μl of water (100 v/v)