C6 - JCSG Top96 CRYO - Screen Contents

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Supplemental Information

Screen Description

Rigaku / JCSG Top96 CRYO

Created 2019-01-17.

Rigaku Recipe XML

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Screen Structure

Well Count	96
Number of distinct ingredients	54
Internal diversity (Score mean dist)	0.91
Internal diversity (Ion mean dist)	0.89
Internal diversity (PEG mean dist)	0.88
Internal diversity (Known pH mean dist)	0.83
Internal diversity (Penalty mean dist)	0.9

Screen Composition

More information about screen composition chart.

Chemical classes bar chart — ingredient type JCSG Top96 CRYO

Buffer 100

Cryoprotectant 25

Organic (non-volatile) 87

Organic (volatile) 4

Polymer 80

Salt 109

ingredient type	JCSG Top96 CRYO
Buffer	100
Cryoprotectant	25
Organic (non-volatile)	87
Organic (volatile)	4
Polymer	80
Salt	109

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

JCSG Top96 CRYO Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	1,2-Propanediol 40 v/v - Sodium acetate 0.1 M pH 4.5 🔍 ⚖️	pH 4.5	400.0 μl of 1,2-Propanediol (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of water (100 v/v)
A2	Ethylene glycol 35 v/v - 🔍 ⚖️	NA	350.0 μl of Ethylene glycol (100 v/v) 650.0 μl of water (100 v/v)
A3	2-methyl-2,4-pentanediol BCC 25 v/v - tri-Sodium citrate 0.1 M pH 2.5 🔍 ⚖️	pH 2.5	250.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 95.11 μl of tri-Sodium citrate pH 1 (1 M) 4.89 μl of tri-Sodium citrate pH 3 (1 M) 650.0 μl of water (100 v/v)
A4	2-methyl-2,4-pentanediol BCC 20 v/v - Sodium acetate 0.1 M pH 5 🔍 ⚖️	pH 5	666.67 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 233.33 μl of water (100 v/v)
A5	2-methyl-2,4-pentanediol BCC 40 v/v - HEPES 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of HEPES pH 6.5 (1 M) 500.0 μl of water (100 v/v)
A6	2-methyl-2,4-pentanediol BCC 65 v/v - BICINE 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of BICINE pH 8.5 (1 M) 250.0 μl of water (100 v/v)
A7	Polyethylene glycol 300 20 v/v - Ammonium sulfate 0.2 M - Glycerol 10 v/v - Sodium phosphate citrate 0.1 M pH 4.2 🔍 ⚖️	pH 4.2	100.0 μl of Ammonium sulfate (2 M) 333.33 μl of Glycerol (30 v/v) 200.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 266.67 μl of water (100 v/v)
A8	Ammonium sulfate 1.7 M - Polyethylene glycol 400 1.7 v/v - Glycerol 15 v/v - HEPES 0.085 M pH 7.5 🔍 ⚖️	pH 7.5	425.0 μl of Ammonium sulfate (4 M) 187.5 μl of Glycerol (80 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 113.33 μl of Polyethylene glycol 400 (15 v/v) 189.17 μl of water (100 v/v)
A9	Polyethylene glycol 4,000 20 w/v - Ammonium sulfate 0.16 M - Glycerol 20 v/v - Sodium acetate 0.08 M pH 4.6 🔍 ⚖️	pH 4.6	160.0 μl of Ammonium sulfate (1 M) 200.0 μl of Glycerol (100 v/v) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 360.0 μl of water (100 v/v)
A10	Polyethylene glycol 4,000 25.5 w/v - Ammonium acetate 0.17 M - Glycerol 15 v/v - tri-Sodium citrate 0.085 M pH 5.6 🔍 ⚖️	pH 5.6	170.0 μl of Ammonium acetate (1 M) 300.0 μl of Glycerol (50 v/v) 255.0 μl of Polyethylene glycol 4,000 (100 w/v) 85.0 μl of tri-Sodium citrate pH 5.6 (1 M) 190.0 μl of water (100 v/v)
A11	Polyethylene glycol 4,000 25.5 w/v - Glycerol 15 v/v - Sodium acetate 0.17 M - tris chloride BCC 0.085 M pH 8.5 🔍 ⚖️	pH 8.5	187.5 μl of Glycerol (80 v/v) 255.0 μl of Polyethylene glycol 4,000 (100 w/v) 85.0 μl of Sodium acetate (2 M) 85.0 μl of tris chloride BCC pH 8.5 (1 M) 387.5 μl of water (100 v/v)
A12	Polyethylene glycol 8,000 16 w/v - Glycerol 20 v/v - Magnesium acetate 0.16 M - Sodium cacodylate 0.08 M pH 6.5 🔍 ⚖️	pH 6.5	200.0 μl of Glycerol (100 v/v) 160.0 μl of Magnesium acetate (1 M) 320.0 μl of Polyethylene glycol 8,000 (50 w/v) 80.0 μl of Sodium cacodylate pH 6.5 (1 M) 240.0 μl of water (100 v/v)
B1	Polyethylene glycol 400 30 v/v - Polyethylene glycol 3,000 5 w/v - Glycerol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	125.0 μl of Glycerol (80 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 300.0 μl of Polyethylene glycol 400 (100 v/v) 375.0 μl of water (100 v/v)
B2	Polyethylene glycol 600 30 w/v - Polyethylene glycol 1,000 5 w/v - Glycerol 10 v/v - MES 0.1 M pH 6 🔍 ⚖️	pH 6	200.0 μl of Glycerol (50 v/v) 100.0 μl of MES pH 6 (1 M) 100.0 μl of Polyethylene glycol 1,000 (50 w/v) 400.0 μl of Polyethylene glycol 600 (75 w/v) 200.0 μl of water (100 v/v)
B3	Glycerol 30 v/v - Polyethylene glycol 4,000 5.6 w/v - Sodium acetate 0.07 M pH 4.6 🔍 ⚖️	pH 4.6	375.0 μl of Glycerol (80 v/v) 112.0 μl of Polyethylene glycol 4,000 (50 w/v) 70.0 μl of Sodium acetate pH 4.6 (1 M) 443.0 μl of water (100 v/v)
B4	Polyethylene glycol 4,000 17 w/v - 2-Propanol 8.5 v/v - Glycerol 15 v/v - Ethylene glycol 10 v/v - HEPES 0.085 M pH 7.5 🔍 ⚖️	pH 7.5	283.33 μl of 2-Propanol (30 v/v) 100.0 μl of Ethylene glycol (100 v/v) 187.5 μl of Glycerol (80 v/v) 85.0 μl of HEPES pH 7.5 (1 M) 340.0 μl of Polyethylene glycol 4,000 (50 w/v) 4.17 μl of water (100 v/v)
B5	2-Propanol 19 v/v - Polyethylene glycol 4,000 19 w/v - Glycerol 5 v/v - tri-Sodium citrate 0.095 M pH 5.6 🔍 ⚖️	pH 5.6	190.0 μl of 2-Propanol (100 v/v) 100.0 μl of Glycerol (50 v/v) 380.0 μl of Polyethylene glycol 4,000 (50 w/v) 95.0 μl of tri-Sodium citrate pH 5.6 (1 M) 235.0 μl of water (100 v/v)
B6	Polyethylene glycol 4,000 20 w/v - 2-Propanol 10 v/v - Glycerol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of 2-Propanol (100 v/v) 100.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 300.0 μl of water (100 v/v)
B7	2-Propanol 20 v/v - Polyethylene glycol 4,000 20 w/v - Ethylene glycol 10 v/v - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	200.0 μl of 2-Propanol (100 v/v) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 4,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 200.0 μl of water (100 v/v)
B8	2-methyl-2,4-pentanediol BCC 40 v/v - Polyethylene glycol 8,000 5 w/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	400.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v)
B9	Ammonium sulfate 1.6 M - Glycerol 20 v/v - Sodium acetate 0.08 M pH 4.6 🔍 ⚖️	pH 4.6	444.44 μl of Ammonium sulfate (3.6 M) 200.0 μl of Glycerol (100 v/v) 80.0 μl of Sodium acetate pH 4.6 (1 M) 275.56 μl of water (100 v/v)
B10	2-methyl-2,4-pentanediol BCC 30 v/v - Sodium chloride 0.2 M - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	300.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 100.0 μl of Sodium chloride (2 M) 500.0 μl of water (100 v/v)
B11	2-methyl-2,4-pentanediol BCC 35 v/v - Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 66.67 μl of Ammonium acetate (3 M) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 483.33 μl of water (100 v/v)
B12	2-methyl-2,4-pentanediol BCC 35 v/v - Lithium sulfate 0.2 M - MES 0.1 M pH 6 🔍 ⚖️	pH 6	350.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v) 100.0 μl of Lithium sulfate (2 M) 100.0 μl of MES pH 6 (1 M) 450.0 μl of water (100 v/v)
C1	Polyethylene glycol 200 50 v/v - tris chloride BCC 0.1 M pH 7 🔍 ⚖️	pH 7	500.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of tris chloride BCC pH 7 (1 M) 400.0 μl of water (100 v/v)
C2	Polyethylene glycol 400 40 v/v - Polyethylene glycol 3,000 5 w/v - MES 0.1 M pH 6 🔍 ⚖️	pH 6	100.0 μl of MES pH 6 (1 M) 100.0 μl of Polyethylene glycol 3,000 (50 w/v) 400.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v)
C3	Polyethylene glycol 600 40 w/v - CHES 0.1 M pH 9.5 🔍 ⚖️	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 533.33 μl of Polyethylene glycol 600 (75 w/v) 366.67 μl of water (100 v/v)
C4	Polyethylene glycol 3,000 20 w/v - Ethylene glycol 10 v/v - tri-Sodium citrate 0.1 M pH 5.5 🔍 ⚖️	pH 5.5	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 400.0 μl of water (100 v/v)
C5	Polyethylene glycol 200 25 v/v - Polyethylene glycol 6,000 5 w/v - tri-Sodium citrate 0.1 M pH 4 🔍 ⚖️	pH 4	250.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 550.0 μl of water (100 v/v)
C6	Polyethylene glycol 6,000 20 w/v - Glycerol 15 v/v - tri-Sodium citrate 0.1 M pH 4 🔍 ⚖️	pH 4	150.0 μl of Glycerol (100 v/v) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 350.0 μl of water (100 v/v)
C7	Polyethylene glycol 6,000 20 w/v - Ethylene glycol 10 v/v - BICINE 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	100.0 μl of BICINE pH 8.5 (1 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 400.0 μl of water (100 v/v)
C8	Polyethylene glycol 6,000 30 w/v - Polyethylene glycol 200 10 v/v - MES 0.1 M pH 5 🔍 ⚖️	pH 5	100.0 μl of MES pH 5 (1 M) 100.0 μl of Polyethylene glycol 200 (100 v/v) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
C9	Polyethylene glycol 6,000 30 w/v - Ethylene glycol 5 v/v - BICINE 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	100.0 μl of BICINE pH 8.5 (1 M) 50.0 μl of Ethylene glycol (100 v/v) 600.0 μl of Polyethylene glycol 6,000 (50 w/v) 250.0 μl of water (100 v/v)
C10	Polyethylene glycol 8,000 20 w/v - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 400.0 μl of water (100 v/v)
C11	Polyethylene glycol 8,000 20 w/v - Polyethylene glycol 200 12 v/v - CHES 0.1 M pH 9.5 🔍 ⚖️	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 120.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 380.0 μl of water (100 v/v)
C12	Glycerol 20 v/v - Ammonium sulfate 0.8 M - tri-Sodium citrate 0.1 M pH 3.5 🔍 ⚖️	pH 3.5	266.67 μl of Ammonium sulfate (3 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of tri-Sodium citrate pH 3.5 (1 M) 433.33 μl of water (100 v/v)
D1	Ammonium sulfate 1.6 M - Glycerol 20 v/v - tri-Sodium citrate 0.1 M pH 4 🔍 ⚖️	pH 4	457.14 μl of Ammonium sulfate (3.5 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of tri-Sodium citrate pH 4 (1 M) 242.86 μl of water (100 v/v)
D2	Ammonium sulfate 1.6 M - Glycerol 20 v/v - MES 0.1 M pH 5 🔍 ⚖️	pH 5	457.14 μl of Ammonium sulfate (3.5 M) 200.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 5 (1 M) 242.86 μl of water (100 v/v)
D3	Ammonium sulfate 1.6 M - Glycerol 15 v/v - BICINE 0.1 M pH 9 🔍 ⚖️	pH 9	457.14 μl of Ammonium sulfate (3.5 M) 100.0 μl of BICINE pH 9 (1 M) 150.0 μl of Glycerol (100 v/v) 292.86 μl of water (100 v/v)
D4	Ammonium sulfate 2 M - Sodium chloride 0.2 M - Ethylene glycol 15 v/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	571.43 μl of Ammonium sulfate (3.5 M) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 40.0 μl of Sodium chloride (5 M) 138.57 μl of water (100 v/v)
D5	Ammonium sulfate 2 M - Ethylene glycol 15 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	571.43 μl of Ammonium sulfate (3.5 M) 150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 178.57 μl of water (100 v/v)
D6	Ammonium sulfate 2 M - Lithium sulfate 0.2 M - Ethylene glycol 15 v/v - CAPS 0.1 M pH 10.5 🔍 ⚖️	pH 10.5	571.43 μl of Ammonium sulfate (3.5 M) 100.0 μl of CAPS pH 10.5 (1 M) 150.0 μl of Ethylene glycol (100 v/v) 66.67 μl of Lithium sulfate (3 M) 111.9 μl of water (100 v/v)
D7	Ammonium sulfate 2.4 M - Glycerol 15 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	685.71 μl of Ammonium sulfate (3.5 M) 150.0 μl of Glycerol (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 64.29 μl of water (100 v/v)
D8	Ammonium sulfate 2.4 M - Glycerol 20 v/v - BICINE 0.1 M pH 9 🔍 ⚖️	pH 9	480.0 μl of Ammonium sulfate (5 M) 100.0 μl of BICINE pH 9 (1 M) 200.0 μl of Glycerol (100 v/v) 220.0 μl of water (100 v/v)
D9	Sodium chloride 2.5 M - Lithium sulfate 0.2 M - Ethylene glycol 10 v/v - Sodium acetate 0.1 M pH 4.5 🔍 ⚖️	pH 4.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Lithium sulfate (2 M) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of Sodium chloride (5 M) 200.0 μl of water (100 v/v)
D10	tri-Sodium citrate 1 M - Glycerol 15 v/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	150.0 μl of Glycerol (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 625.0 μl of tri-Sodium citrate (1.6 M) 125.0 μl of water (100 v/v)
D11	tri-Sodium citrate 1 M - Sodium chloride 0.2 M - Ethylene glycol 10 v/v - tris chloride BCC 0.1 M pH 7 🔍 ⚖️	pH 7	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Sodium chloride (2 M) 500.0 μl of tri-Sodium citrate pH 6.5 (2 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 200.0 μl of water (100 v/v)
D12	tri-Sodium citrate 1 M - Ethylene glycol 10 v/v - CHES 0.1 M pH 9.5 🔍 ⚖️	pH 9.5	100.0 μl of CHES pH 9.5 (1 M) 100.0 μl of Ethylene glycol (100 v/v) 625.0 μl of tri-Sodium citrate (1.6 M) 175.0 μl of water (100 v/v)
E1	tri-Sodium citrate 1.4 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 466.67 μl of tri-Sodium citrate (3 M) 333.33 μl of water (100 v/v)
E2	Sodium Phosphate Monobasic 1.2 M - Lithium sulfate 0.2 M - di-Potassium hydrogen phosphate 0.8 M - Glycerol 25 v/v - CAPS 0.1 M pH 10.5 🔍 ⚖️	pH 10.5	100.0 μl of CAPS pH 10.5 (1 M) 160.0 μl of di-Potassium hydrogen phosphate (5 M) 250.0 μl of Glycerol (100 v/v) 40.0 μl of Lithium sulfate (5 M) 400.0 μl of Sodium Phosphate Monobasic (3 M) 50.0 μl of water (100 v/v)
E3	Polyethylene glycol 200 50 v/v - Sodium chloride 0.2 M - Sodium phosphate citrate 0.1 M pH 4.2 🔍 ⚖️	pH 4.2	500.0 μl of Polyethylene glycol 200 (100 v/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 360.0 μl of water (100 v/v)
E4	Polyethylene glycol 300 40 v/v - Calcium acetate 0.2 M - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	100.0 μl of Calcium acetate (2 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 400.0 μl of water (100 v/v)
E5	Polyethylene glycol 400 28 v/v - Calcium chloride 0.2 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of Calcium chloride (2 M) 100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 420.0 μl of water (100 v/v)
E6	Polyethylene glycol 400 30 v/v - Calcium acetate 0.2 M - Sodium acetate 0.1 M pH 4.5 🔍 ⚖️	pH 4.5	200.0 μl of Calcium acetate (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 400.0 μl of water (100 v/v)
E7	Polyethylene glycol 400 45 v/v - Lithium sulfate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	100.0 μl of Lithium sulfate (2 M) 450.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v)
E8	Polyethylene glycol 400 40 v/v - Magnesium chloride 0.2 M - MES 0.1 M pH 5.5 🔍 ⚖️	pH 5.5	100.0 μl of Magnesium chloride (2 M) 100.0 μl of MES pH 5.5 (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v)
E9	Polyethylene glycol 3,000 10 w/v - Magnesium chloride 0.2 M - Ethylene glycol 15 v/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	150.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium chloride (2 M) 200.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 450.0 μl of water (100 v/v)
E10	Polyethylene glycol 3,000 20 w/v - Sodium chloride 0.2 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7.5 🔍 ⚖️	pH 7.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of Polyethylene glycol 3,000 (50 w/v) 100.0 μl of Sodium chloride (2 M) 300.0 μl of water (100 v/v)
E11	Polyethylene glycol 3,000 30 w/v - Sodium chloride 0.2 M - tris chloride BCC 0.1 M pH 7 🔍 ⚖️	pH 7	600.0 μl of Polyethylene glycol 3,000 (50 w/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 7 (1 M) 260.0 μl of water (100 v/v)
E12	Polyethylene glycol 3,350 20 w/v - Ammonium fluoride 0.2 M - Ethylene glycol 15 v/v - 🔍 ⚖️	NA	20.0 μl of Ammonium fluoride (10 M) 150.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 430.0 μl of water (100 v/v)
F1	Polyethylene glycol 3,350 20 w/v - Ammonium formate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	80.0 μl of Ammonium formate (2.5 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 420.0 μl of water (100 v/v)
F2	Polyethylene glycol 3,350 20 w/v - Ammonium sulfate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	200.0 μl of Ammonium sulfate (1 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v)
F3	Polyethylene glycol 3,350 20 w/v - Calcium acetate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	200.0 μl of Calcium acetate (1 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v)
F4	Polyethylene glycol 3,350 20 w/v - Calcium chloride 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Calcium chloride (2 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
F5	Polyethylene glycol 3,350 20 w/v - di-Ammonium hydrogen phosphate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	57.14 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 442.86 μl of water (100 v/v)
F6	Polyethylene glycol 3,350 20 w/v - di-Ammonium tartrate 0.2 M - Ethylene glycol 15 v/v - 🔍 ⚖️	NA	100.0 μl of di-Ammonium tartrate (2 M) 150.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v)
F7	Polyethylene glycol 3,350 20 w/v - di-Sodium hydrogen phosphate 0.2 M - Ethylene glycol 15 v/v - 🔍 ⚖️	NA	100.0 μl of di-Sodium hydrogen phosphate (2 M) 150.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 350.0 μl of water (100 v/v)
F8	Polyethylene glycol 3,350 20 w/v - Magnesium acetate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium acetate (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
F9	Polyethylene glycol 3,350 20 w/v - Magnesium chloride 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium chloride (2 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v)
F10	Polyethylene glycol 3,350 20 w/v - Magnesium formate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Magnesium formate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 300.0 μl of water (100 v/v)
F11	Polyethylene glycol 3,350 20 w/v - Magnesium nitrate 0.2 M - Ethylene glycol 15 v/v - 🔍 ⚖️	NA	150.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Magnesium nitrate (1 M) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 250.0 μl of water (100 v/v)
F12	Polyethylene glycol 3,350 20 w/v - Potassium formate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Potassium formate (2 M) 400.0 μl of water (100 v/v)
G1	Polyethylene glycol 3,350 20 w/v - Potassium nitrate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Potassium nitrate (2 M) 400.0 μl of water (100 v/v)
G2	Polyethylene glycol 3,350 20 w/v - Sodium acetate 0.2 M - Polyethylene glycol 200 10 v/v - 🔍 ⚖️	NA	100.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate (2 M) 400.0 μl of water (100 v/v)
G3	Polyethylene glycol 3,350 20 w/v - Sodium fluoride 0.2 M - Polyethylene glycol 200 15 v/v - 🔍 ⚖️	NA	150.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium fluoride (2 M) 350.0 μl of water (100 v/v)
G4	Polyethylene glycol 3,350 20 w/v - Sodium formate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 50.0 μl of Sodium formate (4 M) 450.0 μl of water (100 v/v)
G5	Polyethylene glycol 3,350 20 w/v - Sodium nitrate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.57 μl of Sodium nitrate (7 M) 471.43 μl of water (100 v/v)
G6	Polyethylene glycol 3,350 20 w/v - Sodium thiocyanate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium thiocyanate (2 M) 400.0 μl of water (100 v/v)
G7	Polyethylene glycol 3,350 20 w/v - tri-Ammonium citrate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 80.0 μl of tri-Ammonium citrate (2.5 M) 420.0 μl of water (100 v/v)
G8	Polyethylene glycol 3,350 20 w/v - tri-Potassium citrate 0.2 M - Ethylene glycol 10 v/v - 🔍 ⚖️	NA	100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 3,350 (50 w/v) 133.33 μl of tri-Potassium citrate (1.5 M) 366.67 μl of water (100 v/v)
G9	Polyethylene glycol 4,000 25 w/v - Ammonium sulfate 0.2 M - Ethylene glycol 10 v/v - Sodium acetate 0.1 M pH 4.6 🔍 ⚖️	pH 4.6	100.0 μl of Ammonium sulfate (2 M) 100.0 μl of Ethylene glycol (100 v/v) 250.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 450.0 μl of water (100 v/v)
G10	Polyethylene glycol 4,000 30 w/v - Ammonium acetate 0.2 M - tri-Sodium citrate 0.1 M pH 5.6 🔍 ⚖️	pH 5.6	200.0 μl of Ammonium acetate (1 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 400.0 μl of water (100 v/v)
G11	Polyethylene glycol 4,000 30 w/v - Lithium sulfate 0.2 M - Ethylene glycol 10 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Lithium sulfate (2 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v)
G12	Polyethylene glycol 4,000 30 w/v - Magnesium chloride 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 520.0 μl of water (100 v/v)
H1	Polyethylene glycol 4,000 30 w/v - Sodium acetate 0.2 M - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	300.0 μl of Polyethylene glycol 4,000 (100 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v)
H2	Polyethylene glycol 6,000 10 w/v - Lithium chloride 1 M - Ethylene glycol 15 v/v - tri-Sodium citrate 0.1 M pH 5 🔍 ⚖️	pH 5	150.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Lithium chloride (5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 350.0 μl of water (100 v/v)
H3	Polyethylene glycol 6,000 10 w/v - Lithium chloride 1 M - Ethylene glycol 15 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	150.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Lithium chloride (5 M) 200.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v)
H4	Polyethylene glycol 6,000 20 w/v - Lithium chloride 1 M - Ethylene glycol 10 v/v - HEPES 0.1 M pH 7 🔍 ⚖️	pH 7	100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 200.0 μl of water (100 v/v)
H5	Polyethylene glycol 6,000 20 w/v - Lithium chloride 1 M - Glycerol 20 v/v - MES 0.1 M pH 6 🔍 ⚖️	pH 6	200.0 μl of Glycerol (100 v/v) 200.0 μl of Lithium chloride (5 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of water (100 v/v)
H6	Polyethylene glycol 6,000 20 w/v - Lithium chloride 1 M - Glycerol 20 v/v - tri-Sodium citrate 0.1 M pH 5 🔍 ⚖️	pH 5	200.0 μl of Glycerol (100 v/v) 200.0 μl of Lithium chloride (5 M) 400.0 μl of Polyethylene glycol 6,000 (50 w/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 100.0 μl of water (100 v/v)
H7	Polyethylene glycol 8,000 10 w/v - Calcium acetate 0.2 M - Ethylene glycol 20 v/v - Imidazole 0.1 M pH 8 🔍 ⚖️	pH 8	200.0 μl of Calcium acetate (1 M) 200.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Imidazole pH 8 (1 M) 200.0 μl of Polyethylene glycol 8,000 (50 w/v) 300.0 μl of water (100 v/v)
H8	Polyethylene glycol 8,000 20 w/v - Calcium acetate 0.2 M - Glycerol 15 v/v - MES 0.1 M pH 6 🔍 ⚖️	pH 6	200.0 μl of Calcium acetate (1 M) 150.0 μl of Glycerol (100 v/v) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 150.0 μl of water (100 v/v)
H9	Polyethylene glycol 8,000 20 w/v - Magnesium acetate 0.2 M - Ethylene glycol 15 v/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	150.0 μl of Ethylene glycol (100 v/v) 200.0 μl of Magnesium acetate (1 M) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 150.0 μl of water (100 v/v)
H10	Polyethylene glycol 8,000 20 w/v - Magnesium chloride 0.2 M - Polyethylene glycol 200 10 v/v - tris chloride BCC 0.1 M pH 8.5 🔍 ⚖️	pH 8.5	80.0 μl of Magnesium chloride (2.5 M) 100.0 μl of Polyethylene glycol 200 (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 320.0 μl of water (100 v/v)
H11	Polyethylene glycol 8,000 20 w/v - Sodium chloride 0.2 M - Ethylene glycol 10 v/v - CAPS 0.1 M pH 10.5 🔍 ⚖️	pH 10.5	100.0 μl of CAPS pH 10.5 (1 M) 100.0 μl of Ethylene glycol (100 v/v) 400.0 μl of Polyethylene glycol 8,000 (50 w/v) 200.0 μl of Sodium chloride (1 M) 200.0 μl of water (100 v/v)
H12	Polyethylene glycol 8,000 30 w/v - Sodium acetate 0.2 M - Ethylene glycol 15 v/v - Sodium cacodylate 0.1 M pH 6.5 🔍 ⚖️	pH 6.5	150.0 μl of Ethylene glycol (100 v/v) 600.0 μl of Polyethylene glycol 8,000 (50 w/v) 100.0 μl of Sodium acetate (2 M) 100.0 μl of Sodium cacodylate pH 6.5 (1 M) 50.0 μl of water (100 v/v)