C6 - Screen Contents - Low Ionic Strength

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Screen Description

Low Ionic Strength

Hampton Research / Low Ionic Strength

Created 2019-02-04.

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Low Ionic Strength

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Screen Structure

Low Ionic Strength

Well Count	24
Number of distinct ingredients	10
Internal diversity (score mean dist)	0.886

Screen Composition

More information about screen composition chart.

ingredient type	Low Ionic Strength
Buffer	18
Polymer	6
Salt	9

Well Contents

Buffers are shown in italics. More information about buffers.

Primary factors are shown in bold. More information about primary factors.

Low Ionic Strength Well Contents 🔍
Well	Ingredient, Concentration, Units, pH	pH	Recipe 1000μl
A1	Potassium chloride 0.05 M pH 2 Polyethylene glycol 3,350 4 w/v - 🔍	pH 2	80.0 μl of Polyethylene glycol 3,350 (50 w/v) 25.0 μl of Potassium chloride pH 2 (2 M) 895.0 μl of water (100 v/v)
A2	tri-Sodium citrate 0.05 M pH 3 Polyethylene glycol 3,350 8 w/v - 🔍	pH 3	160.0 μl of Polyethylene glycol 3,350 (50 w/v) 35.4 μl of tri-Sodium citrate pH 3 (1 M) 14.6 μl of tri-Sodium citrate pH 5.5 (1 M) 790.0 μl of water (100 v/v)
A3	tri-Sodium citrate 0.05 M pH 3.5 Polyethylene glycol 3,350 12 w/v - 🔍	pH 3.5	240.0 μl of Polyethylene glycol 3,350 (50 w/v) 33.8 μl of tri-Sodium citrate pH 3 (1 M) 16.2 μl of tri-Sodium citrate pH 5.5 (1 M) 710.0 μl of water (100 v/v)
A4	tri-Sodium citrate 0.05 M pH 4 Polyethylene glycol 3,350 16 w/v - 🔍	pH 4	320.0 μl of Polyethylene glycol 3,350 (50 w/v) 31.5 μl of tri-Sodium citrate pH 3 (1 M) 18.5 μl of tri-Sodium citrate pH 5.5 (1 M) 630.0 μl of water (100 v/v)
A5	tri-Sodium citrate 0.05 M pH 4.5 Polyethylene glycol 3,350 20 w/v - 🔍	pH 4.5	400.0 μl of Polyethylene glycol 3,350 (50 w/v) 28.3 μl of tri-Sodium citrate pH 3 (1 M) 21.7 μl of tri-Sodium citrate pH 5.5 (1 M) 550.0 μl of water (100 v/v)
A6	tri-Sodium citrate 0.05 M pH 5 Polyethylene glycol 3,350 24 w/v - 🔍	pH 5	480.0 μl of Polyethylene glycol 3,350 (50 w/v) 24.0 μl of tri-Sodium citrate pH 3 (1 M) 26.0 μl of tri-Sodium citrate pH 5.5 (1 M) 470.0 μl of water (100 v/v)
B1	tri-Sodium citrate 0.05 M pH 5.5 🔍	pH 5.5	18.2 μl of tri-Sodium citrate pH 3 (1 M) 31.8 μl of tri-Sodium citrate pH 5.5 (1 M) 950.0 μl of water (100 v/v)
B2	MES 0.05 M pH 6 🔍	pH 6	100.0 μl of MES pH 6 (0.5 M) 900.0 μl of water (100 v/v)
B3	BIS-TRIS 0.05 M pH 6.5 🔍	pH 6.5	50.0 μl of BIS-TRIS pH 6.5 (1 M) 950.0 μl of water (100 v/v)
B4	Imidazole 0.05 M pH 7 🔍	pH 7	50.0 μl of Imidazole pH 7 (1 M) 950.0 μl of water (100 v/v)
B5	HEPES 0.05 M pH 7.5 🔍	pH 7.5	50.0 μl of HEPES pH 7.5 (1 M) 950.0 μl of water (100 v/v)
B6	tris chloride BCC 0.05 M pH 8 🔍	pH 8	50.0 μl of tris chloride BCC pH 8 (1 M) 950.0 μl of water (100 v/v)
C1	tris chloride BCC 0.05 M pH 8.5 🔍	pH 8.5	50.0 μl of tris chloride BCC pH 8.5 (1 M) 950.0 μl of water (100 v/v)
C2	Glycine 0.05 M pH 9 🔍	pH 9	50.0 μl of Glycine pH 9 (1 M) 950.0 μl of water (100 v/v)
C3	Glycine 0.05 M pH 9.5 🔍	pH 9.5	29.3 μl of Glycine pH 9 (1 M) 20.7 μl of Glycine pH 10 (1 M) 950.0 μl of water (100 v/v)
C4	Glycine 0.05 M pH 10 🔍	pH 10	50.0 μl of Glycine pH 10 (1 M) 950.0 μl of water (100 v/v)
C5	di-Sodium hydrogen phosphate 0.05 M pH 11 🔍	pH 11	50.0 μl of di-Sodium hydrogen phosphate pH 12 (1 M) 0.0 μl of di-Sodium hydrogen phosphate pH 7 (1 M) 950.0 μl of water (100 v/v)
C6	di-Sodium hydrogen phosphate 0.05 M pH 12 🔍	pH 12	50.0 μl of di-Sodium hydrogen phosphate pH 12 (1 M) 950.0 μl of water (100 v/v)
D1	🔍	NA	1000.0 μl of water (100 v/v)
D2	🔍	NA	1000.0 μl of water (100 v/v)
D3	🔍	NA	1000.0 μl of water (100 v/v)
D4	🔍	NA	1000.0 μl of water (100 v/v)
D5	🔍	NA	1000.0 μl of water (100 v/v)
D6	🔍	NA	1000.0 μl of water (100 v/v)