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Supplemental Information

Screen Description

Low Ionic Strength

Hampton Research / Low Ionic Strength
Created 2019-02-04.

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Low Ionic Strength

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Low Ionic Strength

    Screen Structure

    Low Ionic Strength

    Well Count 24
    Number of distinct ingredients 10
    Internal diversity (score mean dist) 0.886

    Screen Composition

    More information about screen composition chart.
    2025-06-14T14:03:37.290054 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type Low Ionic Strength
    Buffer 18
    Polymer 6
    Salt 9

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    Low Ionic Strength Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Potassium chloride 0.05 M pH 2
    • Polyethylene glycol 3,350 4 w/v -
    		pH 2
    25 μl of Potassium chloride pH 2 (2 M)
    80 μl of Polyethylene glycol 3,350 (50 w/v)
    895 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.05 M pH 3
    • Polyethylene glycol 3,350 8 w/v -
    		pH 3
    12 μl of tri-Sodium citrate pH 3 (1 M)
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    37 μl of tri-Sodium citrate pH 5.5 (1 M)
    790 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 0.05 M pH 3.5
    • Polyethylene glycol 3,350 12 w/v -
    		pH 3.5
    10 μl of tri-Sodium citrate pH 3 (1 M)
    240 μl of Polyethylene glycol 3,350 (50 w/v)
    39 μl of tri-Sodium citrate pH 5.5 (1 M)
    710 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.05 M pH 4
    • Polyethylene glycol 3,350 16 w/v -
    		pH 4
    8 μl of tri-Sodium citrate pH 3 (1 M)
    320 μl of Polyethylene glycol 3,350 (50 w/v)
    41 μl of tri-Sodium citrate pH 5.5 (1 M)
    630 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 3,350 20 w/v -
    		pH 4.5
    6 μl of tri-Sodium citrate pH 3 (1 M)
    400 μl of Polyethylene glycol 3,350 (50 w/v)
    43 μl of tri-Sodium citrate pH 5.5 (1 M)
    550 μl of water (100 v/v)
    A6
    • tri-Sodium citrate 0.05 M pH 5
    • Polyethylene glycol 3,350 24 w/v -
    		pH 5
    4 μl of tri-Sodium citrate pH 3 (1 M)
    480 μl of Polyethylene glycol 3,350 (50 w/v)
    45 μl of tri-Sodium citrate pH 5.5 (1 M)
    470 μl of water (100 v/v)
    B1
    • tri-Sodium citrate 0.05 M pH 5.5
    		pH 5.5
    2 μl of tri-Sodium citrate pH 3 (1 M)
    47 μl of tri-Sodium citrate pH 5.5 (1 M)
    950 μl of water (100 v/v)
    B2
    • MES 0.05 M pH 6
    		pH 6
    100 μl of MES pH 6 (0.5 M)
    900 μl of water (100 v/v)
    B3
    • BIS-TRIS 0.05 M pH 6.5
    		pH 6.5
    50 μl of BIS-TRIS pH 6.5 (1 M)
    950 μl of water (100 v/v)
    B4
    • Imidazole 0.05 M pH 7
    		pH 7
    50 μl of Imidazole pH 7 (1 M)
    950 μl of water (100 v/v)
    B5
    • HEPES 0.05 M pH 7.5
    		pH 7.5
    50 μl of HEPES pH 7.5 (1 M)
    950 μl of water (100 v/v)
    B6
    • tris chloride BCC 0.05 M pH 8
    		pH 8
    50 μl of tris chloride BCC pH 8 (1 M)
    950 μl of water (100 v/v)
    C1
    • tris chloride BCC 0.05 M pH 8.5
    		pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    950 μl of water (100 v/v)
    C2
    • Glycine 0.05 M pH 9
    		pH 9
    50 μl of Glycine pH 9 (1 M)
    950 μl of water (100 v/v)
    C3
    • Glycine 0.05 M pH 9.5
    		pH 9.5
    29 μl of Glycine pH 9 (1 M)
    20 μl of Glycine pH 10 (1 M)
    950 μl of water (100 v/v)
    C4
    • Glycine 0.05 M pH 10
    		pH 10
    50 μl of Glycine pH 10 (1 M)
    950 μl of water (100 v/v)
    C5
    • di-Sodium hydrogen phosphate 0.05 M pH 11
    		pH 11
    50 μl of di-Sodium hydrogen phosphate pH 12 (1 M)
    0 μl of di-Sodium hydrogen phosphate pH 7 (1 M)
    950 μl of water (100 v/v)
    C6
    • di-Sodium hydrogen phosphate 0.05 M pH 12
    		pH 12
    50 μl of di-Sodium hydrogen phosphate pH 12 (1 M)
    950 μl of water (100 v/v)
    D1NA
    check warnings
    D2NA
    check warnings
    D3NA
    check warnings
    D4NA
    check warnings
    D5NA
    check warnings
    D6NA
    check warnings