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Supplemental Information

Screen Description

PEG Ion 400

Hampton Research / PEG Ion 400
Created 2019-02-28.

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PEG Ion 400

    Screen Structure

    PEG Ion 400

    Well Count 96
    Number of distinct ingredients 49
    Internal diversity (score mean dist) 0.660

    Screen Composition

    2025-06-13T21:33:16.586918 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG Ion 400
    Buffer 50
    Complex ingredient 2
    Organic (non-volatile) 96
    Polymer 96
    Salt 92

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG Ion 400 Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Polyethylene glycol 400 30 v/v -
    • Ammonium acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium acetate pH 7.1 (1 M)
    600 μl of water (100 v/v)
    A2
    • Polyethylene glycol 400 30 v/v -
    • Ammonium acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ammonium acetate pH 7.3 (1 M)
    300 μl of water (100 v/v)
    A3
    • Polyethylene glycol 400 30 v/v -
    • Calcium acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium acetate pH 7.2 (1 M)
    600 μl of water (100 v/v)
    A4
    • Polyethylene glycol 400 30 v/v -
    • Calcium acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Calcium acetate pH 7.4 (1 M)
    300 μl of water (100 v/v)
    A5
    • Polyethylene glycol 400 30 v/v -
    • Lithium acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium acetate pH 7.2 (1 M)
    600 μl of water (100 v/v)
    A6
    • Polyethylene glycol 400 30 v/v -
    • Lithium acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium acetate pH 7.5 (1 M)
    300 μl of water (100 v/v)
    A7
    • Polyethylene glycol 400 30 v/v -
    • Magnesium acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium acetate pH 7.2 (1 M)
    600 μl of water (100 v/v)
    A8
    • Polyethylene glycol 400 30 v/v -
    • Magnesium acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Magnesium acetate pH 7.3 (1 M)
    300 μl of water (100 v/v)
    A9
    • Polyethylene glycol 400 30 v/v -
    • Potassium acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Potassium acetate pH 7.3 (1 M)
    600 μl of water (100 v/v)
    A10
    • Polyethylene glycol 400 30 v/v -
    • Potassium acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Potassium acetate pH 7.7 (1 M)
    300 μl of water (100 v/v)
    A11
    • Polyethylene glycol 400 30 v/v -
    • Sodium acetate 0.1 M pH 7.2
    pH 7.2
    300 μl of Polyethylene glycol 400 (100 v/v)
    36 μl of Sodium acetate pH 6.8 (1 M)
    64 μl of Sodium acetate pH 8 (1 M)
    600 μl of water (100 v/v)
    A12
    • Polyethylene glycol 400 30 v/v -
    • Sodium acetate 0.4 M pH 7.6
    pH 7.6
    300 μl of Polyethylene glycol 400 (100 v/v)
    41 μl of Sodium acetate pH 6.8 (1 M)
    358 μl of Sodium acetate pH 8 (1 M)
    300 μl of water (100 v/v)
    B1
    • Polyethylene glycol 400 30 v/v -
    • Zinc acetate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Zinc acetate pH 6.5 (1 M)
    600 μl of water (100 v/v)
    B2
    • Polyethylene glycol 400 30 v/v -
    • Zinc acetate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Zinc acetate pH 6.1 (1 M)
    300 μl of water (100 v/v)
    B3
    • Polyethylene glycol 400 30 v/v -
    • Tetraethylammonium bromide 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Tetraethylammonium bromide pH 6 (1 M)
    600 μl of water (100 v/v)
    B4
    • Polyethylene glycol 400 30 v/v -
    • Tetraethylammonium bromide 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Tetraethylammonium bromide pH 6.1 (1 M)
    300 μl of water (100 v/v)
    B5
    • Polyethylene glycol 400 30 v/v -
    • Ammonium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium chloride pH 5.9 (1 M)
    600 μl of water (100 v/v)
    B6
    • Polyethylene glycol 400 30 v/v -
    • Ammonium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ammonium chloride pH 5.8 (1 M)
    300 μl of water (100 v/v)
    B7
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Cadmium chloride pH 5.4 (1 M)
    600 μl of water (100 v/v)
    B8
    • Polyethylene glycol 400 30 v/v -
    • Cadmium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Cadmium chloride pH 4.9 (1 M)
    300 μl of water (100 v/v)
    B9
    • Polyethylene glycol 400 30 v/v -
    • Calcium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium chloride pH 5.7 (1 M)
    600 μl of water (100 v/v)
    B10
    • Polyethylene glycol 400 30 v/v -
    • Calcium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Calcium chloride pH 5.3 (1 M)
    300 μl of water (100 v/v)
    B11
    • Polyethylene glycol 400 30 v/v -
    • Lithium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium chloride pH 5.9 (1 M)
    600 μl of water (100 v/v)
    B12
    • Polyethylene glycol 400 30 v/v -
    • Lithium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium chloride pH 5.8 (1 M)
    300 μl of water (100 v/v)
    C1
    • Polyethylene glycol 400 30 v/v -
    • Magnesium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium chloride pH 5.7 (1 M)
    600 μl of water (100 v/v)
    C2
    • Polyethylene glycol 400 30 v/v -
    • Magnesium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Magnesium chloride pH 5.3 (1 M)
    300 μl of water (100 v/v)
    C3
    • Polyethylene glycol 400 30 v/v -
    • Nickel (II) chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Nickel (II) chloride pH 5.5 (1 M)
    600 μl of water (100 v/v)
    C4
    • Polyethylene glycol 400 30 v/v -
    • Nickel (II) chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Nickel (II) chloride pH 5 (1 M)
    300 μl of water (100 v/v)
    C5
    • Polyethylene glycol 400 30 v/v -
    • Potassium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Potassium chloride pH 6 (1 M)
    600 μl of water (100 v/v)
    C6
    • Polyethylene glycol 400 30 v/v -
    • Potassium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Potassium chloride pH 5.9 (1 M)
    300 μl of water (100 v/v)
    C7
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium chloride pH 6 (1 M)
    600 μl of water (100 v/v)
    C8
    • Polyethylene glycol 400 30 v/v -
    • Sodium chloride 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Sodium chloride pH 5.8 (1 M)
    300 μl of water (100 v/v)
    C9
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium hydrogen citrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Ammonium hydrogen citrate pH 5.6 (1 M)
    600 μl of water (100 v/v)
    C10
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium hydrogen citrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of di-Ammonium hydrogen citrate pH 5.3 (1 M)
    300 μl of water (100 v/v)
    C11
    • Polyethylene glycol 400 30 v/v -
    • tri-Ammonium citrate 0.1 M pH 7.3
    pH 7.3
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of tri-Ammonium citrate pH 7.5 (3 M)
    0 μl of tri-Ammonium citrate pH 7 (3 M)
    666 μl of water (100 v/v)
    C12
    • Polyethylene glycol 400 30 v/v -
    • tri-Ammonium citrate 0.4 M pH 7.2
    pH 7.2
    300 μl of Polyethylene glycol 400 (100 v/v)
    132 μl of tri-Ammonium citrate pH 7.5 (3 M)
    1 μl of tri-Ammonium citrate pH 7 (3 M)
    566 μl of water (100 v/v)
    D1
    • Polyethylene glycol 400 30 v/v -
    • tri-Lithium citrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tri-Lithium citrate pH 7.6 (1 M)
    600 μl of water (100 v/v)
    D2
    • Polyethylene glycol 400 30 v/v -
    • tri-Lithium citrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of tri-Lithium citrate pH 7.7 (1 M)
    300 μl of water (100 v/v)
    D3
    • Polyethylene glycol 400 30 v/v -
    • tri-Potassium citrate 0.1 M pH 7.9
    pH 7.9
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tri-Potassium citrate pH 6.5 (5 M)
    20 μl of tri-Potassium citrate pH 8.3 (5 M)
    680 μl of water (100 v/v)
    D4
    • Polyethylene glycol 400 30 v/v -
    • tri-Potassium citrate 0.4 M pH 8.2
    pH 8.2
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tri-Potassium citrate pH 6.5 (5 M)
    79 μl of tri-Potassium citrate pH 8.3 (5 M)
    620 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.1 M pH 7.7
    pH 7.7
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tri-Sodium citrate pH 7.5 (1 M)
    100 μl of tri-Sodium citrate pH 8 (1 M)
    600 μl of water (100 v/v)
    D6
    • Polyethylene glycol 400 30 v/v -
    • tri-Sodium citrate 0.4 M pH 7.8
    pH 7.8
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tri-Sodium citrate pH 7.5 (1 M)
    400 μl of tri-Sodium citrate pH 8 (1 M)
    300 μl of water (100 v/v)
    D7
    • Polyethylene glycol 400 30 v/v -
    • Ammonium formate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium formate pH 6.4 (1 M)
    600 μl of water (100 v/v)
    D8
    • Polyethylene glycol 400 30 v/v -
    • Ammonium formate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ammonium formate pH 6.6 (1 M)
    300 μl of water (100 v/v)
    D9
    • Polyethylene glycol 400 30 v/v -
    • Magnesium formate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium formate pH 6.4 (1 M)
    600 μl of water (100 v/v)
    D10
    • Polyethylene glycol 400 30 v/v -
    • Magnesium formate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Magnesium formate pH 6.5 (1 M)
    300 μl of water (100 v/v)
    D11
    • Polyethylene glycol 400 30 v/v -
    • Potassium formate 0.1 M pH 6.4
    pH 6.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    77 μl of Potassium formate pH 6.3 (1 M)
    22 μl of Potassium formate pH 7.3 (1 M)
    600 μl of water (100 v/v)
    D12
    • Polyethylene glycol 400 30 v/v -
    • Potassium formate 0.4 M pH 6.7
    pH 6.7
    300 μl of Polyethylene glycol 400 (100 v/v)
    135 μl of Potassium formate pH 6.3 (1 M)
    265 μl of Potassium formate pH 7.3 (1 M)
    300 μl of water (100 v/v)
    E1
    • Polyethylene glycol 400 30 v/v -
    • Sodium formate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium formate pH 6.5 (1 M)
    600 μl of water (100 v/v)
    E2
    • Polyethylene glycol 400 30 v/v -
    • Sodium formate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Sodium formate pH 6.8 (1 M)
    300 μl of water (100 v/v)
    E3
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium DL-malate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Sodium DL-malate pH 7.1 (1 M)
    600 μl of water (100 v/v)
    E4
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium DL-malate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of di-Sodium DL-malate pH 7.1 (1 M)
    300 μl of water (100 v/v)
    E5
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium malonate 0.1 M pH 7.6
    pH 7.6
    300 μl of Polyethylene glycol 400 (100 v/v)
    24 μl of di-Sodium malonate pH 8 (3.4 M)
    5 μl of di-Sodium malonate pH 7 (3.4 M)
    670 μl of water (100 v/v)
    E6
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium malonate 0.4 M pH 7.5
    pH 7.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    88 μl of di-Sodium malonate pH 8 (3.4 M)
    28 μl of di-Sodium malonate pH 7 (3.4 M)
    582 μl of water (100 v/v)
    E7
    • Polyethylene glycol 400 30 v/v -
    • Ammonium nitrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium nitrate pH 6 (1 M)
    600 μl of water (100 v/v)
    E8
    • Polyethylene glycol 400 30 v/v -
    • Ammonium nitrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ammonium nitrate pH 5.8 (1 M)
    300 μl of water (100 v/v)
    E9
    • Polyethylene glycol 400 30 v/v -
    • Ethylammonium nitrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ethylammonium nitrate pH 6 (1 M)
    600 μl of water (100 v/v)
    E10
    • Polyethylene glycol 400 30 v/v -
    • Ethylammonium nitrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ethylammonium nitrate pH 5.8 (1 M)
    300 μl of water (100 v/v)
    E11
    • Polyethylene glycol 400 30 v/v -
    • Lithium nitrate 0.1 M -
    NA
    100 μl of Lithium nitrate pH 5.9 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    600 μl of water (100 v/v)
    E12
    • Polyethylene glycol 400 30 v/v -
    • Lithium nitrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium nitrate pH 5.7 (1 M)
    300 μl of water (100 v/v)
    F1
    • Polyethylene glycol 400 30 v/v -
    • Magnesium nitrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium nitrate pH 5.7 (1 M)
    600 μl of water (100 v/v)
    F2
    • Polyethylene glycol 400 30 v/v -
    • Magnesium nitrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Magnesium nitrate pH 5.3 (1 M)
    300 μl of water (100 v/v)
    F3
    • Polyethylene glycol 400 30 v/v -
    • Sodium nitrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium nitrate pH 6 (1 M)
    600 μl of water (100 v/v)
    F4
    • Polyethylene glycol 400 30 v/v -
    • Sodium nitrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Sodium nitrate pH 5.8 (1 M)
    300 μl of water (100 v/v)
    F5
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium hydrogen phosphate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Ammonium hydrogen phosphate pH 8.2 (1 M)
    600 μl of water (100 v/v)
    F6
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium hydrogen phosphate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of di-Ammonium hydrogen phosphate pH 8.2 (1 M)
    300 μl of water (100 v/v)
    F7
    • Polyethylene glycol 400 30 v/v -
    • Ammonium di-hydrogen phosphate 0.1 M pH 5.4
    pH 5.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    1 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    34 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    664 μl of water (100 v/v)
    F8
    • Polyethylene glycol 400 30 v/v -
    • Ammonium di-hydrogen phosphate 0.4 M pH 4.8
    pH 4.8
    300 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of Ammonium di-hydrogen phosphate pH 7 (2.8 M)
    142 μl of Ammonium di-hydrogen phosphate pH 4.6 (2.8 M)
    557 μl of water (100 v/v)
    F9
    • Polyethylene glycol 400 30 v/v -
    • di-Potassium hydrogen phosphate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Potassium hydrogen phosphate pH 9 (1 M)
    600 μl of water (100 v/v)
    F10
    • Polyethylene glycol 400 30 v/v -
    • di-Potassium hydrogen phosphate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of di-Potassium hydrogen phosphate pH 9.3 (1 M)
    300 μl of water (100 v/v)
    F11
    • Polyethylene glycol 400 30 v/v -
    • Potassium di-hydrogen phosphate 0.1 M pH 5.5
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    1 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
    48 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M)
    650 μl of water (100 v/v)
    F12
    • Polyethylene glycol 400 30 v/v -
    • Potassium di-hydrogen phosphate 0.4 M pH 5
    pH 5
    300 μl of Polyethylene glycol 400 (100 v/v)
    1 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
    198 μl of Potassium di-hydrogen phosphate pH 4.6 (2 M)
    500 μl of water (100 v/v)
    G1
    • Polyethylene glycol 400 30 v/v -
    • Sodium Potassium phosphate 0.1 M pH 7.7
    pH 7.7
    300 μl of Polyethylene glycol 400 (100 v/v)
    33 μl of Sodium Potassium phosphate pH 8.2 (3 M)
    0 μl of Sodium Potassium phosphate pH 7.5 (3 M)
    666 μl of water (100 v/v)
    G2
    • Polyethylene glycol 400 30 v/v -
    • Sodium Potassium phosphate 0.4 M pH 7.4
    pH 7.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    2 μl of Sodium Potassium phosphate pH 7 (3 M)
    131 μl of Sodium Potassium phosphate pH 8.2 (3 M)
    566 μl of water (100 v/v)
    G3
    • Polyethylene glycol 400 30 v/v -
    • Sodium di-hydrogen phosphate 0.1 M pH 5.5
    pH 5.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    23 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M)
    1 μl of Sodium di-hydrogen phosphate pH 7 (4 M)
    675 μl of water (100 v/v)
    G4
    • Polyethylene glycol 400 30 v/v -
    • Sodium di-hydrogen phosphate 0.4 M pH 4.9
    pH 4.9
    300 μl of Polyethylene glycol 400 (100 v/v)
    99 μl of Sodium di-hydrogen phosphate pH 4.6 (4 M)
    0 μl of Sodium di-hydrogen phosphate pH 7 (4 M)
    600 μl of water (100 v/v)
    G5
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium succinate 0.1 M pH 7.4
    pH 7.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    87 μl of di-Sodium succinate pH 7.5 (1 M)
    12 μl of di-Sodium succinate pH 7 (1 M)
    600 μl of water (100 v/v)
    G6
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium succinate 0.4 M pH 7.4
    pH 7.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    351 μl of di-Sodium succinate pH 7.5 (1 M)
    49 μl of di-Sodium succinate pH 7 (1 M)
    300 μl of water (100 v/v)
    G7
    • Polyethylene glycol 400 30 v/v -
    • Ammonium sulfate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Ammonium sulfate pH 5.7 (1 M)
    600 μl of water (100 v/v)
    G8
    • Polyethylene glycol 400 30 v/v -
    • Ammonium sulfate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Ammonium sulfate pH 5.6 (1 M)
    300 μl of water (100 v/v)
    G9
    • Polyethylene glycol 400 30 v/v -
    • Lithium sulfate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium sulfate pH 5.8 (1 M)
    600 μl of water (100 v/v)
    G10
    • Polyethylene glycol 400 30 v/v -
    • Lithium sulfate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Lithium sulfate pH 5.5 (1 M)
    300 μl of water (100 v/v)
    G11
    • Polyethylene glycol 400 30 v/v -
    • Magnesium sulfate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Magnesium sulfate pH 5.9 (1 M)
    600 μl of water (100 v/v)
    G12
    • Polyethylene glycol 400 30 v/v -
    • Magnesium sulfate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Magnesium sulfate pH 5.6 (1 M)
    300 μl of water (100 v/v)
    H1
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium tartrate 0.1 M pH 6.5
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    44 μl of di-Ammonium tartrate pH 8.5 (2 M)
    5 μl of di-Ammonium tartrate pH 5.5 (2 M)
    650 μl of water (100 v/v)
    H2
    • Polyethylene glycol 400 30 v/v -
    • di-Ammonium tartrate 0.4 M pH 6.6
    pH 6.6
    300 μl of Polyethylene glycol 400 (100 v/v)
    183 μl of di-Ammonium tartrate pH 8.5 (2 M)
    16 μl of di-Ammonium tartrate pH 5.5 (2 M)
    500 μl of water (100 v/v)
    H3
    • Polyethylene glycol 400 30 v/v -
    • Potassium Sodium tartrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Potassium Sodium tartrate pH 6.7 (1 M)
    600 μl of water (100 v/v)
    H4
    • Polyethylene glycol 400 30 v/v -
    • Potassium Sodium tartrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Potassium Sodium tartrate pH 7 (1 M)
    300 μl of water (100 v/v)
    H5
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium tartrate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of di-Sodium tartrate pH 6.6 (1 M)
    600 μl of water (100 v/v)
    H6
    • Polyethylene glycol 400 30 v/v -
    • di-Sodium tartrate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of di-Sodium tartrate pH 6.9 (1 M)
    300 μl of water (100 v/v)
    H7
    • Polyethylene glycol 400 30 v/v -
    • Potassium thiocyanate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Potassium thiocyanate pH 6.1 (1 M)
    600 μl of water (100 v/v)
    H8
    • Polyethylene glycol 400 30 v/v -
    • Potassium thiocyanate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Potassium thiocyanate pH 6 (1 M)
    300 μl of water (100 v/v)
    H9
    • Polyethylene glycol 400 30 v/v -
    • Sodium thiocyanate 0.1 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Sodium thiocyanate pH 6.1 (1 M)
    600 μl of water (100 v/v)
    H10
    • Polyethylene glycol 400 30 v/v -
    • Sodium thiocyanate 0.4 M -
    NA
    300 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of Sodium thiocyanate pH 6.1 (1 M)
    300 μl of water (100 v/v)
    H11
    • Polyethylene glycol 400 30 v/v -
    • Tacsimate 5 v/v pH 7.4
    pH 7.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    49 μl of Tacsimate pH 8 (100 v/v)
    0 μl of Tacsimate pH 7 (100 v/v)
    650 μl of water (100 v/v)
    H12
    • Polyethylene glycol 400 30 v/v -
    • Tacsimate 20 v/v pH 7.4
    pH 7.4
    300 μl of Polyethylene glycol 400 (100 v/v)
    199 μl of Tacsimate pH 8 (100 v/v)
    0 μl of Tacsimate pH 7 (100 v/v)
    500 μl of water (100 v/v)