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Supplemental Information

Screen Description

PEG pH HT

Hampton Research / PEG pH HT
Created 2019-07-03.

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PEG pH HT

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PEG pH HT

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Screen Structure

PEG pH HT

Well Count 96
Number of distinct ingredients 18
Internal diversity (score mean dist) 0.631

Screen Composition

More information about screen composition chart.
2025-08-11T21:33:36.588822 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type PEG pH HT
Buffer 96
Polymer 96
Salt 28

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
PEG pH HT Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • tri-Sodium citrate 0.2 M pH 3.5
  • Polyethylene glycol 4,000 20 w/v -
pH 3.5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
64.9 μl of tri-Sodium citrate pH 4.5 (1 M)
135.1 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A2
  • tri-Sodium citrate 0.2 M pH 3.8
  • Polyethylene glycol 4,000 20 w/v -
pH 3.8
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
70.2 μl of tri-Sodium citrate pH 4.5 (1 M)
129.8 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A3
  • tri-Sodium citrate 0.2 M pH 4.1
  • Polyethylene glycol 4,000 20 w/v -
pH 4.1
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
76.3 μl of tri-Sodium citrate pH 4.5 (1 M)
123.7 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A4
  • tri-Sodium citrate 0.2 M pH 4.4
  • Polyethylene glycol 4,000 20 w/v -
pH 4.4
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
84.0 μl of tri-Sodium citrate pH 4.5 (1 M)
116.0 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A5
  • tri-Sodium citrate 0.2 M pH 3.6
  • Polyethylene glycol 4,000 20 w/v -
pH 3.6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
66.4 μl of tri-Sodium citrate pH 4.5 (1 M)
133.6 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A6
  • tri-Sodium citrate 0.2 M pH 3.9
  • Polyethylene glycol 4,000 20 w/v -
pH 3.9
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
71.9 μl of tri-Sodium citrate pH 4.5 (1 M)
128.1 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A7
  • tri-Sodium citrate 0.2 M pH 4.2
  • Polyethylene glycol 4,000 20 w/v -
pH 4.2
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
78.7 μl of tri-Sodium citrate pH 4.5 (1 M)
121.3 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A8
  • tri-Sodium citrate 0.2 M pH 4.5
  • Polyethylene glycol 4,000 20 w/v -
pH 4.5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
87.0 μl of tri-Sodium citrate pH 4.5 (1 M)
113.0 μl of tri-Sodium citrate pH 3.5 (1 M)
600.0 μl of water (100 v/v)
A9
  • Sodium acetate 0.2 M pH 3.7
  • Polyethylene glycol 4,000 20 w/v -
pH 3.7
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
200.0 μl of Sodium acetate pH 3.7 (1 M)
0.0 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
A10
  • Sodium acetate 0.2 M pH 4
  • Polyethylene glycol 4,000 20 w/v -
pH 4
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
176.6 μl of Sodium acetate pH 3.7 (1 M)
23.4 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
A11
  • Sodium acetate 0.2 M pH 4.3
  • Polyethylene glycol 4,000 20 w/v -
pH 4.3
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
138.2 μl of Sodium acetate pH 3.7 (1 M)
61.8 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
A12
  • Sodium acetate 0.2 M pH 4.6
  • Polyethylene glycol 4,000 20 w/v -
pH 4.6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
83.9 μl of Sodium acetate pH 3.7 (1 M)
116.1 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
B1
  • Sodium acetate 0.2 M pH 4.9
  • Polyethylene glycol 4,000 20 w/v -
pH 4.9
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
20.7 μl of Sodium acetate pH 3.7 (1 M)
179.3 μl of Sodium acetate pH 5 (1 M)
600.0 μl of water (100 v/v)
B2
  • di-Sodium DL-malate 0.2 M pH 4.7
  • Polyethylene glycol 4,000 20 w/v -
pH 4.7
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
25.7 μl of di-Sodium DL-malate pH 2.9 (3 M)
41.0 μl of di-Sodium DL-malate pH 7 (3 M)
733.3 μl of water (100 v/v)
B3
  • di-Sodium DL-malate 0.2 M pH 5
  • Polyethylene glycol 4,000 20 w/v -
pH 5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
24.3 μl of di-Sodium DL-malate pH 2.9 (3 M)
42.4 μl of di-Sodium DL-malate pH 7 (3 M)
733.3 μl of water (100 v/v)
B4
  • di-Sodium DL-malate 0.2 M pH 5.3
  • Polyethylene glycol 4,000 20 w/v -
pH 5.3
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
23.3 μl of di-Sodium DL-malate pH 2.9 (3 M)
43.3 μl of di-Sodium DL-malate pH 7 (3 M)
733.3 μl of water (100 v/v)
B5
  • di-Sodium DL-malate 0.2 M pH 5.6
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
22.8 μl of di-Sodium DL-malate pH 2.9 (3 M)
43.9 μl of di-Sodium DL-malate pH 7 (3 M)
733.3 μl of water (100 v/v)
B6
  • di-Sodium DL-malate 0.2 M pH 5.9
  • Polyethylene glycol 4,000 20 w/v -
pH 5.9
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
22.5 μl of di-Sodium DL-malate pH 2.9 (3 M)
44.2 μl of di-Sodium DL-malate pH 7 (3 M)
733.3 μl of water (100 v/v)
B7
  • di-Sodium succinate 0.2 M pH 4.8
  • Polyethylene glycol 4,000 20 w/v -
pH 4.8
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
184.5 μl of di-Sodium succinate pH 4.5 (1 M)
15.5 μl of di-Sodium succinate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
B8
  • di-Sodium succinate 0.2 M pH 5.1
  • Polyethylene glycol 4,000 20 w/v -
pH 5.1
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
159.1 μl of di-Sodium succinate pH 4.5 (1 M)
40.9 μl of di-Sodium succinate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
B9
  • di-Sodium succinate 0.2 M pH 5.4
  • Polyethylene glycol 4,000 20 w/v -
pH 5.4
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
123.1 μl of di-Sodium succinate pH 4.5 (1 M)
76.9 μl of di-Sodium succinate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
B10
  • di-Sodium succinate 0.2 M pH 5.7
  • Polyethylene glycol 4,000 20 w/v -
pH 5.7
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
81.2 μl of di-Sodium succinate pH 4.5 (1 M)
118.8 μl of di-Sodium succinate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
B11
  • di-Sodium succinate 0.2 M pH 6
  • Polyethylene glycol 4,000 20 w/v -
pH 6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
42.5 μl of di-Sodium succinate pH 4.5 (1 M)
157.5 μl of di-Sodium succinate pH 6.5 (1 M)
600.0 μl of water (100 v/v)
B12
  • Sodium cacodylate 0.2 M pH 5.2
  • Polyethylene glycol 4,000 20 w/v -
pH 5.2
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
188.6 μl of Sodium cacodylate pH 5 (1 M)
11.4 μl of Sodium cacodylate pH 6.4 (1 M)
600.0 μl of water (100 v/v)
C1
  • Sodium cacodylate 0.2 M pH 5.5
  • Polyethylene glycol 4,000 20 w/v -
pH 5.5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
161.4 μl of Sodium cacodylate pH 5 (1 M)
38.6 μl of Sodium cacodylate pH 6.4 (1 M)
600.0 μl of water (100 v/v)
C2
  • Sodium cacodylate 0.2 M pH 5.8
  • Polyethylene glycol 4,000 20 w/v -
pH 5.8
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
118.6 μl of Sodium cacodylate pH 5 (1 M)
81.4 μl of Sodium cacodylate pH 6.4 (1 M)
600.0 μl of water (100 v/v)
C3
  • Sodium cacodylate 0.2 M pH 6.1
  • Polyethylene glycol 4,000 20 w/v -
pH 6.1
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
61.6 μl of Sodium cacodylate pH 5 (1 M)
138.4 μl of Sodium cacodylate pH 6.4 (1 M)
600.0 μl of water (100 v/v)
C4
  • Sodium cacodylate 0.2 M pH 6.4
  • Polyethylene glycol 4,000 20 w/v -
pH 6.4
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
-0.0 μl of Sodium cacodylate pH 5 (1 M)
200.0 μl of Sodium cacodylate pH 6.4 (1 M)
600.0 μl of water (100 v/v)
C5
  • MES 0.2 M pH 5.3
  • Polyethylene glycol 4,000 20 w/v -
pH 5.3
180.3 μl of MES pH 5 (1 M)
19.7 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C6
  • MES 0.2 M pH 5.6
  • Polyethylene glycol 4,000 20 w/v -
pH 5.6
148.1 μl of MES pH 5 (1 M)
51.9 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C7
  • MES 0.2 M pH 5.9
  • Polyethylene glycol 4,000 20 w/v -
pH 5.9
102.4 μl of MES pH 5 (1 M)
97.6 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C8
  • MES 0.2 M pH 6.2
  • Polyethylene glycol 4,000 20 w/v -
pH 6.2
49.2 μl of MES pH 5 (1 M)
150.8 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C9
  • MES 0.2 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
0.0 μl of MES pH 5 (1 M)
200.0 μl of MES pH 6.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C10
  • BIS-TRIS 0.2 M pH 5.7
  • Polyethylene glycol 4,000 20 w/v -
pH 5.7
13.7 μl of BIS-TRIS pH 7 (1 M)
186.3 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C11
  • BIS-TRIS 0.2 M pH 6
  • Polyethylene glycol 4,000 20 w/v -
pH 6
44.7 μl of BIS-TRIS pH 7 (1 M)
155.3 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
C12
  • BIS-TRIS 0.2 M pH 6.3
  • Polyethylene glycol 4,000 20 w/v -
pH 6.3
88.5 μl of BIS-TRIS pH 7 (1 M)
111.5 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D1
  • BIS-TRIS 0.2 M pH 6.6
  • Polyethylene glycol 4,000 20 w/v -
pH 6.6
139.5 μl of BIS-TRIS pH 7 (1 M)
60.5 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D2
  • BIS-TRIS 0.2 M pH 6.9
  • Polyethylene glycol 4,000 20 w/v -
pH 6.9
186.7 μl of BIS-TRIS pH 7 (1 M)
13.3 μl of BIS-TRIS pH 5.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D3
  • ADA 0.2 M pH 5.8
  • Polyethylene glycol 4,000 20 w/v -
pH 5.8
180.3 μl of ADA pH 5.5 (1 M)
19.7 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D4
  • ADA 0.2 M pH 6.1
  • Polyethylene glycol 4,000 20 w/v -
pH 6.1
148.1 μl of ADA pH 5.5 (1 M)
51.9 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D5
  • ADA 0.2 M pH 6.4
  • Polyethylene glycol 4,000 20 w/v -
pH 6.4
102.4 μl of ADA pH 5.5 (1 M)
97.6 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D6
  • ADA 0.2 M pH 6.7
  • Polyethylene glycol 4,000 20 w/v -
pH 6.7
49.2 μl of ADA pH 5.5 (1 M)
150.8 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D7
  • ADA 0.2 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
0.0 μl of ADA pH 5.5 (1 M)
200.0 μl of ADA pH 7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D8
  • Imidazole 0.2 M pH 6.2
  • Polyethylene glycol 4,000 20 w/v -
pH 6.2
200.0 μl of Imidazole pH 6.2 (1 M)
0.0 μl of Imidazole pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D9
  • Imidazole 0.2 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
166.8 μl of Imidazole pH 6.2 (1 M)
33.2 μl of Imidazole pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D10
  • Imidazole 0.2 M pH 6.8
  • Polyethylene glycol 4,000 20 w/v -
pH 6.8
119.7 μl of Imidazole pH 6.2 (1 M)
80.3 μl of Imidazole pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D11
  • Imidazole 0.2 M pH 7.1
  • Polyethylene glycol 4,000 20 w/v -
pH 7.1
65.0 μl of Imidazole pH 6.2 (1 M)
135.0 μl of Imidazole pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
D12
  • Imidazole 0.2 M pH 7.4
  • Polyethylene glycol 4,000 20 w/v -
pH 7.4
14.3 μl of Imidazole pH 6.2 (1 M)
185.7 μl of Imidazole pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E1
  • BIS-TRIS propane 0.2 M pH 6.4
  • Polyethylene glycol 4,000 20 w/v -
pH 6.4
-0.0 μl of BIS-TRIS propane pH 7.5 (1 M)
200.0 μl of BIS-TRIS propane pH 6.4 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E2
  • BIS-TRIS propane 0.2 M pH 6.7
  • Polyethylene glycol 4,000 20 w/v -
pH 6.7
57.5 μl of BIS-TRIS propane pH 7.5 (1 M)
142.5 μl of BIS-TRIS propane pH 6.4 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E3
  • BIS-TRIS propane 0.2 M pH 7
  • Polyethylene glycol 4,000 20 w/v -
pH 7
119.7 μl of BIS-TRIS propane pH 7.5 (1 M)
80.3 μl of BIS-TRIS propane pH 6.4 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E4
  • BIS-TRIS propane 0.2 M pH 7.3
  • Polyethylene glycol 4,000 20 w/v -
pH 7.3
173.1 μl of BIS-TRIS propane pH 7.5 (1 M)
26.9 μl of BIS-TRIS propane pH 6.4 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E5
  • MOPS 0.2 M pH 6.5
  • Polyethylene glycol 4,000 20 w/v -
pH 6.5
200.0 μl of MOPS pH 6.5 (1 M)
0.0 μl of MOPS pH 7.9 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E6
  • MOPS 0.2 M pH 6.8
  • Polyethylene glycol 4,000 20 w/v -
pH 6.8
164.5 μl of MOPS pH 6.5 (1 M)
35.5 μl of MOPS pH 7.9 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E7
  • MOPS 0.2 M pH 7.1
  • Polyethylene glycol 4,000 20 w/v -
pH 7.1
117.2 μl of MOPS pH 6.5 (1 M)
82.8 μl of MOPS pH 7.9 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E8
  • MOPS 0.2 M pH 7.4
  • Polyethylene glycol 4,000 20 w/v -
pH 7.4
66.1 μl of MOPS pH 6.5 (1 M)
133.9 μl of MOPS pH 7.9 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E9
  • MOPS 0.2 M pH 7.7
  • Polyethylene glycol 4,000 20 w/v -
pH 7.7
22.2 μl of MOPS pH 6.5 (1 M)
177.8 μl of MOPS pH 7.9 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E10
  • HEPES 0.2 M pH 6.6
  • Polyethylene glycol 4,000 20 w/v -
pH 6.6
200.0 μl of HEPES pH 6.6 (1 M)
0.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E11
  • HEPES 0.2 M pH 6.9
  • Polyethylene glycol 4,000 20 w/v -
pH 6.9
154.2 μl of HEPES pH 6.6 (1 M)
45.8 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
E12
  • HEPES 0.2 M pH 7.2
  • Polyethylene glycol 4,000 20 w/v -
pH 7.2
85.6 μl of HEPES pH 6.6 (1 M)
114.4 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F1
  • HEPES 0.2 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
-0.0 μl of HEPES pH 6.6 (1 M)
200.0 μl of HEPES pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F2
  • HEPES 0.2 M pH 6.8
  • Polyethylene glycol 4,000 20 w/v -
pH 6.8
177.4 μl of HEPES pH 6.5 (1 M)
22.6 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F3
  • HEPES 0.2 M pH 7.1
  • Polyethylene glycol 4,000 20 w/v -
pH 7.1
142.0 μl of HEPES pH 6.5 (1 M)
58.0 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F4
  • HEPES 0.2 M pH 7.4
  • Polyethylene glycol 4,000 20 w/v -
pH 7.4
94.8 μl of HEPES pH 6.5 (1 M)
105.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F5
  • HEPES 0.2 M pH 7.7
  • Polyethylene glycol 4,000 20 w/v -
pH 7.7
43.8 μl of HEPES pH 6.5 (1 M)
156.2 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
F6
  • tris chloride BCC 0.2 M pH 7.2
  • Polyethylene glycol 4,000 20 w/v -
pH 7.2
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
188.1 μl of tris chloride BCC pH 7 (1 M)
11.9 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F7
  • tris chloride BCC 0.2 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
160.4 μl of tris chloride BCC pH 7 (1 M)
39.6 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F8
  • tris chloride BCC 0.2 M pH 7.8
  • Polyethylene glycol 4,000 20 w/v -
pH 7.8
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
118.9 μl of tris chloride BCC pH 7 (1 M)
81.1 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F9
  • tris chloride BCC 0.2 M pH 8.1
  • Polyethylene glycol 4,000 20 w/v -
pH 8.1
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
67.1 μl of tris chloride BCC pH 7 (1 M)
132.9 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F10
  • tris chloride BCC 0.2 M pH 7.3
  • Polyethylene glycol 4,000 20 w/v -
pH 7.3
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
180.3 μl of tris chloride BCC pH 7 (1 M)
19.7 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F11
  • tris chloride BCC 0.2 M pH 7.6
  • Polyethylene glycol 4,000 20 w/v -
pH 7.6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
148.1 μl of tris chloride BCC pH 7 (1 M)
51.9 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
F12
  • tris chloride BCC 0.2 M pH 7.9
  • Polyethylene glycol 4,000 20 w/v -
pH 7.9
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
102.4 μl of tris chloride BCC pH 7 (1 M)
97.6 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
G1
  • tris chloride BCC 0.2 M pH 8.2
  • Polyethylene glycol 4,000 20 w/v -
pH 8.2
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
49.2 μl of tris chloride BCC pH 7 (1 M)
150.8 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
G2
  • tris chloride BCC 0.2 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
0.0 μl of tris chloride BCC pH 7 (1 M)
200.0 μl of tris chloride BCC pH 8.5 (1 M)
600.0 μl of water (100 v/v)
G3
  • TRICINE 0.2 M pH 7.4
  • Polyethylene glycol 4,000 20 w/v -
pH 7.4
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
200.0 μl of TRICINE pH 7.4 (1 M)
0.0 μl of TRICINE pH 8.8 (1 M)
600.0 μl of water (100 v/v)
G4
  • TRICINE 0.2 M pH 7.7
  • Polyethylene glycol 4,000 20 w/v -
pH 7.7
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
164.5 μl of TRICINE pH 7.4 (1 M)
35.5 μl of TRICINE pH 8.8 (1 M)
600.0 μl of water (100 v/v)
G5
  • TRICINE 0.2 M pH 8
  • Polyethylene glycol 4,000 20 w/v -
pH 8
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
117.2 μl of TRICINE pH 7.4 (1 M)
82.8 μl of TRICINE pH 8.8 (1 M)
600.0 μl of water (100 v/v)
G6
  • TRICINE 0.2 M pH 8.3
  • Polyethylene glycol 4,000 20 w/v -
pH 8.3
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
66.1 μl of TRICINE pH 7.4 (1 M)
133.9 μl of TRICINE pH 8.8 (1 M)
600.0 μl of water (100 v/v)
G7
  • TRICINE 0.2 M pH 8.6
  • Polyethylene glycol 4,000 20 w/v -
pH 8.6
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
22.2 μl of TRICINE pH 7.4 (1 M)
177.8 μl of TRICINE pH 8.8 (1 M)
600.0 μl of water (100 v/v)
G8
  • BICINE 0.2 M pH 7.5
  • Polyethylene glycol 4,000 20 w/v -
pH 7.5
0.0 μl of BICINE pH 8.8 (1 M)
200.0 μl of BICINE pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
G9
  • BICINE 0.2 M pH 7.8
  • Polyethylene glycol 4,000 20 w/v -
pH 7.8
33.2 μl of BICINE pH 8.8 (1 M)
166.8 μl of BICINE pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
G10
  • BICINE 0.2 M pH 8.1
  • Polyethylene glycol 4,000 20 w/v -
pH 8.1
80.3 μl of BICINE pH 8.8 (1 M)
119.7 μl of BICINE pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
G11
  • BICINE 0.2 M pH 8.4
  • Polyethylene glycol 4,000 20 w/v -
pH 8.4
135.0 μl of BICINE pH 8.8 (1 M)
65.0 μl of BICINE pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
G12
  • BICINE 0.2 M pH 8.7
  • Polyethylene glycol 4,000 20 w/v -
pH 8.7
185.7 μl of BICINE pH 8.8 (1 M)
14.3 μl of BICINE pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H1
  • BIS-TRIS propane 0.2 M pH 8.5
  • Polyethylene glycol 4,000 20 w/v -
pH 8.5
200.0 μl of BIS-TRIS propane pH 8.5 (1 M)
0.0 μl of BIS-TRIS propane pH 9.7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H2
  • BIS-TRIS propane 0.2 M pH 8.8
  • Polyethylene glycol 4,000 20 w/v -
pH 8.8
94.4 μl of BIS-TRIS propane pH 8.5 (1 M)
105.6 μl of BIS-TRIS propane pH 9.7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H3
  • BIS-TRIS propane 0.2 M pH 9.1
  • Polyethylene glycol 4,000 20 w/v -
pH 9.1
40.7 μl of BIS-TRIS propane pH 8.5 (1 M)
159.3 μl of BIS-TRIS propane pH 9.7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H4
  • BIS-TRIS propane 0.2 M pH 9.4
  • Polyethylene glycol 4,000 20 w/v -
pH 9.4
13.6 μl of BIS-TRIS propane pH 8.5 (1 M)
186.4 μl of BIS-TRIS propane pH 9.7 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H5
  • Glycine 0.2 M pH 8.6
  • Polyethylene glycol 4,000 20 w/v -
pH 8.6
172.5 μl of Glycine pH 8 (1 M)
27.5 μl of Glycine pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H6
  • Glycine 0.2 M pH 8.9
  • Polyethylene glycol 4,000 20 w/v -
pH 8.9
139.5 μl of Glycine pH 8 (1 M)
60.5 μl of Glycine pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H7
  • Glycine 0.2 M pH 9.2
  • Polyethylene glycol 4,000 20 w/v -
pH 9.2
83.6 μl of Glycine pH 8 (1 M)
116.4 μl of Glycine pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H8
  • Glycine 0.2 M pH 9.5
  • Polyethylene glycol 4,000 20 w/v -
pH 9.5
0.0 μl of Glycine pH 8 (1 M)
200.0 μl of Glycine pH 9.5 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H9
  • AMPD 0.2 M pH 8.7
  • Polyethylene glycol 4,000 20 w/v -
pH 8.7
200.0 μl of AMPD pH 8.7 (1 M)
0.0 μl of AMPD pH 10 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H10
  • AMPD 0.2 M pH 9
  • Polyethylene glycol 4,000 20 w/v -
pH 9
131.5 μl of AMPD pH 8.7 (1 M)
68.5 μl of AMPD pH 10 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H11
  • AMPD 0.2 M pH 9.3
  • Polyethylene glycol 4,000 20 w/v -
pH 9.3
72.7 μl of AMPD pH 8.7 (1 M)
127.3 μl of AMPD pH 10 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)
H12
  • AMPD 0.2 M pH 9.6
  • Polyethylene glycol 4,000 20 w/v -
pH 9.6
31.1 μl of AMPD pH 8.7 (1 M)
168.9 μl of AMPD pH 10 (1 M)
200.0 μl of Polyethylene glycol 4,000 (100 w/v)
600.0 μl of water (100 v/v)