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Supplemental Information

Screen Description

PEG pH HT

Hampton Research / PEG pH HT
Created 2019-07-03.

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PEG pH HT

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PEG pH HT

    Screen Structure

    PEG pH HT

    Well Count 96
    Number of distinct ingredients 18
    Internal diversity (score mean dist) 0.631

    Screen Composition

    More information about screen composition chart.
    2025-06-14T13:45:43.990510 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type PEG pH HT
    Buffer 96
    Polymer 96
    Salt 28

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    PEG pH HT Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.2 M pH 3.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 3.5
    158 μl of tri-Sodium citrate pH 4.5 (1 M)
    41 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A2
    • tri-Sodium citrate 0.2 M pH 3.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 3.8
    163 μl of tri-Sodium citrate pH 4.5 (1 M)
    37 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A3
    • tri-Sodium citrate 0.2 M pH 4.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.1
    167 μl of tri-Sodium citrate pH 4.5 (1 M)
    32 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A4
    • tri-Sodium citrate 0.2 M pH 4.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.4
    172 μl of tri-Sodium citrate pH 4.5 (1 M)
    27 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A5
    • tri-Sodium citrate 0.2 M pH 3.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 3.6
    159 μl of tri-Sodium citrate pH 4.5 (1 M)
    40 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A6
    • tri-Sodium citrate 0.2 M pH 3.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 3.9
    164 μl of tri-Sodium citrate pH 4.5 (1 M)
    35 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A7
    • tri-Sodium citrate 0.2 M pH 4.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.2
    169 μl of tri-Sodium citrate pH 4.5 (1 M)
    30 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A8
    • tri-Sodium citrate 0.2 M pH 4.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.5
    174 μl of tri-Sodium citrate pH 4.5 (1 M)
    26 μl of tri-Sodium citrate pH 3.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A9
    • Sodium acetate 0.2 M pH 3.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 3.7
    0 μl of Sodium acetate pH 5 (1 M)
    200 μl of Sodium acetate pH 3.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A10
    • Sodium acetate 0.2 M pH 4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4
    23 μl of Sodium acetate pH 5 (1 M)
    176 μl of Sodium acetate pH 3.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A11
    • Sodium acetate 0.2 M pH 4.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.3
    61 μl of Sodium acetate pH 5 (1 M)
    138 μl of Sodium acetate pH 3.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    A12
    • Sodium acetate 0.2 M pH 4.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.6
    116 μl of Sodium acetate pH 5 (1 M)
    83 μl of Sodium acetate pH 3.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B1
    • Sodium acetate 0.2 M pH 4.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.9
    179 μl of Sodium acetate pH 5 (1 M)
    20 μl of Sodium acetate pH 3.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B2
    • di-Sodium DL-malate 0.2 M pH 4.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.7
    62 μl of di-Sodium DL-malate pH 7 (3 M)
    4 μl of di-Sodium DL-malate pH 2.9 (3 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    733 μl of water (100 v/v)
    B3
    • di-Sodium DL-malate 0.2 M pH 5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5
    62 μl of di-Sodium DL-malate pH 7 (3 M)
    3 μl of di-Sodium DL-malate pH 2.9 (3 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    733 μl of water (100 v/v)
    B4
    • di-Sodium DL-malate 0.2 M pH 5.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.3
    63 μl of di-Sodium DL-malate pH 7 (3 M)
    3 μl of di-Sodium DL-malate pH 2.9 (3 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    733 μl of water (100 v/v)
    B5
    • di-Sodium DL-malate 0.2 M pH 5.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.6
    63 μl of di-Sodium DL-malate pH 7 (3 M)
    3 μl of di-Sodium DL-malate pH 2.9 (3 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    733 μl of water (100 v/v)
    B6
    • di-Sodium DL-malate 0.2 M pH 5.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.9
    63 μl of di-Sodium DL-malate pH 7 (3 M)
    3 μl of di-Sodium DL-malate pH 2.9 (3 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    733 μl of water (100 v/v)
    B7
    • di-Sodium succinate 0.2 M pH 4.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 4.8
    15 μl of di-Sodium succinate pH 6.5 (1 M)
    184 μl of di-Sodium succinate pH 4.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B8
    • di-Sodium succinate 0.2 M pH 5.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.1
    40 μl of di-Sodium succinate pH 6.5 (1 M)
    159 μl of di-Sodium succinate pH 4.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B9
    • di-Sodium succinate 0.2 M pH 5.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.4
    76 μl of di-Sodium succinate pH 6.5 (1 M)
    123 μl of di-Sodium succinate pH 4.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B10
    • di-Sodium succinate 0.2 M pH 5.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.7
    118 μl of di-Sodium succinate pH 6.5 (1 M)
    81 μl of di-Sodium succinate pH 4.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B11
    • di-Sodium succinate 0.2 M pH 6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6
    157 μl of di-Sodium succinate pH 6.5 (1 M)
    42 μl of di-Sodium succinate pH 4.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    B12
    • Sodium cacodylate 0.2 M pH 5.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.2
    11 μl of Sodium cacodylate pH 6.4 (1 M)
    188 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C1
    • Sodium cacodylate 0.2 M pH 5.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.5
    38 μl of Sodium cacodylate pH 6.4 (1 M)
    161 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C2
    • Sodium cacodylate 0.2 M pH 5.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.8
    81 μl of Sodium cacodylate pH 6.4 (1 M)
    118 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C3
    • Sodium cacodylate 0.2 M pH 6.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.1
    138 μl of Sodium cacodylate pH 6.4 (1 M)
    61 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C4
    • Sodium cacodylate 0.2 M pH 6.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.4
    200 μl of Sodium cacodylate pH 6.4 (1 M)
    0 μl of Sodium cacodylate pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C5
    • MES 0.2 M pH 5.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.3
    19 μl of MES pH 6.5 (1 M)
    180 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C6
    • MES 0.2 M pH 5.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.6
    51 μl of MES pH 6.5 (1 M)
    148 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C7
    • MES 0.2 M pH 5.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.9
    97 μl of MES pH 6.5 (1 M)
    102 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C8
    • MES 0.2 M pH 6.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.2
    150 μl of MES pH 6.5 (1 M)
    49 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C9
    • MES 0.2 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.5
    200 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C10
    • BIS-TRIS 0.2 M pH 5.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.7
    13 μl of BIS-TRIS pH 7 (1 M)
    186 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C11
    • BIS-TRIS 0.2 M pH 6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6
    44 μl of BIS-TRIS pH 7 (1 M)
    155 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    C12
    • BIS-TRIS 0.2 M pH 6.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.3
    88 μl of BIS-TRIS pH 7 (1 M)
    111 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D1
    • BIS-TRIS 0.2 M pH 6.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.6
    139 μl of BIS-TRIS pH 7 (1 M)
    60 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D2
    • BIS-TRIS 0.2 M pH 6.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.9
    186 μl of BIS-TRIS pH 7 (1 M)
    13 μl of BIS-TRIS pH 5.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D3
    • ADA 0.2 M pH 5.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 5.8
    19 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    180 μl of ADA pH 5.5 (1 M)
    600 μl of water (100 v/v)
    D4
    • ADA 0.2 M pH 6.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.1
    51 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    148 μl of ADA pH 5.5 (1 M)
    600 μl of water (100 v/v)
    D5
    • ADA 0.2 M pH 6.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.4
    97 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    102 μl of ADA pH 5.5 (1 M)
    600 μl of water (100 v/v)
    D6
    • ADA 0.2 M pH 6.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.7
    150 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    49 μl of ADA pH 5.5 (1 M)
    600 μl of water (100 v/v)
    D7
    • ADA 0.2 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7
    200 μl of ADA pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    0 μl of ADA pH 5.5 (1 M)
    600 μl of water (100 v/v)
    D8
    • Imidazole 0.2 M pH 6.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.2
    200 μl of Imidazole pH 6.2 (1 M)
    0 μl of Imidazole pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D9
    • Imidazole 0.2 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.5
    166 μl of Imidazole pH 6.2 (1 M)
    33 μl of Imidazole pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D10
    • Imidazole 0.2 M pH 6.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.8
    119 μl of Imidazole pH 6.2 (1 M)
    80 μl of Imidazole pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D11
    • Imidazole 0.2 M pH 7.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.1
    65 μl of Imidazole pH 6.2 (1 M)
    135 μl of Imidazole pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    D12
    • Imidazole 0.2 M pH 7.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.4
    14 μl of Imidazole pH 6.2 (1 M)
    185 μl of Imidazole pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E1
    • BIS-TRIS propane 0.2 M pH 6.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.4
    200 μl of BIS-TRIS propane pH 6.4 (1 M)
    0 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E2
    • BIS-TRIS propane 0.2 M pH 6.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.7
    142 μl of BIS-TRIS propane pH 6.4 (1 M)
    57 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E3
    • BIS-TRIS propane 0.2 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7
    80 μl of BIS-TRIS propane pH 6.4 (1 M)
    119 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E4
    • BIS-TRIS propane 0.2 M pH 7.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.3
    26 μl of BIS-TRIS propane pH 6.4 (1 M)
    173 μl of BIS-TRIS propane pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E5
    • MOPS 0.2 M pH 6.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.5
    0 μl of MOPS pH 7.9 (1 M)
    200 μl of MOPS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E6
    • MOPS 0.2 M pH 6.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.8
    35 μl of MOPS pH 7.9 (1 M)
    164 μl of MOPS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E7
    • MOPS 0.2 M pH 7.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.1
    82 μl of MOPS pH 7.9 (1 M)
    117 μl of MOPS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E8
    • MOPS 0.2 M pH 7.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.4
    133 μl of MOPS pH 7.9 (1 M)
    66 μl of MOPS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E9
    • MOPS 0.2 M pH 7.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.7
    177 μl of MOPS pH 7.9 (1 M)
    22 μl of MOPS pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E10
    • HEPES 0.2 M pH 6.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.6
    0 μl of HEPES pH 7.5 (1 M)
    200 μl of HEPES pH 6.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E11
    • HEPES 0.2 M pH 6.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.9
    45 μl of HEPES pH 7.5 (1 M)
    154 μl of HEPES pH 6.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    E12
    • HEPES 0.2 M pH 7.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.2
    114 μl of HEPES pH 7.5 (1 M)
    85 μl of HEPES pH 6.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F1
    • HEPES 0.2 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.5
    200 μl of HEPES pH 7.5 (1 M)
    0 μl of HEPES pH 6.6 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F2
    • HEPES 0.2 M pH 6.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 6.8
    22 μl of HEPES pH 8 (1 M)
    177 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F3
    • HEPES 0.2 M pH 7.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.1
    58 μl of HEPES pH 8 (1 M)
    142 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F4
    • HEPES 0.2 M pH 7.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.4
    105 μl of HEPES pH 8 (1 M)
    94 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F5
    • HEPES 0.2 M pH 7.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.7
    156 μl of HEPES pH 8 (1 M)
    43 μl of HEPES pH 6.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F6
    • tris chloride BCC 0.2 M pH 7.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.2
    11 μl of tris chloride BCC pH 8.5 (1 M)
    188 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F7
    • tris chloride BCC 0.2 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.5
    39 μl of tris chloride BCC pH 8.5 (1 M)
    160 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F8
    • tris chloride BCC 0.2 M pH 7.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.8
    81 μl of tris chloride BCC pH 8.5 (1 M)
    118 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F9
    • tris chloride BCC 0.2 M pH 8.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.1
    132 μl of tris chloride BCC pH 8.5 (1 M)
    67 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F10
    • tris chloride BCC 0.2 M pH 7.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.3
    19 μl of tris chloride BCC pH 8.5 (1 M)
    180 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F11
    • tris chloride BCC 0.2 M pH 7.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.6
    51 μl of tris chloride BCC pH 8.5 (1 M)
    148 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    F12
    • tris chloride BCC 0.2 M pH 7.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.9
    97 μl of tris chloride BCC pH 8.5 (1 M)
    102 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G1
    • tris chloride BCC 0.2 M pH 8.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.2
    150 μl of tris chloride BCC pH 8.5 (1 M)
    49 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G2
    • tris chloride BCC 0.2 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.5
    200 μl of tris chloride BCC pH 8.5 (1 M)
    0 μl of tris chloride BCC pH 7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G3
    • TRICINE 0.2 M pH 7.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.4
    200 μl of TRICINE pH 7.4 (1 M)
    0 μl of TRICINE pH 8.8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G4
    • TRICINE 0.2 M pH 7.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.7
    164 μl of TRICINE pH 7.4 (1 M)
    35 μl of TRICINE pH 8.8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G5
    • TRICINE 0.2 M pH 8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8
    117 μl of TRICINE pH 7.4 (1 M)
    82 μl of TRICINE pH 8.8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G6
    • TRICINE 0.2 M pH 8.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.3
    66 μl of TRICINE pH 7.4 (1 M)
    133 μl of TRICINE pH 8.8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G7
    • TRICINE 0.2 M pH 8.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.6
    22 μl of TRICINE pH 7.4 (1 M)
    177 μl of TRICINE pH 8.8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G8
    • BICINE 0.2 M pH 7.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.5
    0 μl of BICINE pH 8.8 (1 M)
    200 μl of BICINE pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G9
    • BICINE 0.2 M pH 7.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 7.8
    33 μl of BICINE pH 8.8 (1 M)
    166 μl of BICINE pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G10
    • BICINE 0.2 M pH 8.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.1
    80 μl of BICINE pH 8.8 (1 M)
    119 μl of BICINE pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G11
    • BICINE 0.2 M pH 8.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.4
    135 μl of BICINE pH 8.8 (1 M)
    65 μl of BICINE pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    G12
    • BICINE 0.2 M pH 8.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.7
    185 μl of BICINE pH 8.8 (1 M)
    14 μl of BICINE pH 7.5 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H1
    • BIS-TRIS propane 0.2 M pH 8.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.5
    200 μl of BIS-TRIS propane pH 8.5 (1 M)
    0 μl of BIS-TRIS propane pH 9.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H2
    • BIS-TRIS propane 0.2 M pH 8.8
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.8
    94 μl of BIS-TRIS propane pH 8.5 (1 M)
    105 μl of BIS-TRIS propane pH 9.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H3
    • BIS-TRIS propane 0.2 M pH 9.1
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.1
    40 μl of BIS-TRIS propane pH 8.5 (1 M)
    159 μl of BIS-TRIS propane pH 9.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H4
    • BIS-TRIS propane 0.2 M pH 9.4
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.4
    13 μl of BIS-TRIS propane pH 8.5 (1 M)
    186 μl of BIS-TRIS propane pH 9.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H5
    • Glycine 0.2 M pH 8.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.6
    27 μl of Glycine pH 9.5 (1 M)
    172 μl of Glycine pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H6
    • Glycine 0.2 M pH 8.9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.9
    60 μl of Glycine pH 9.5 (1 M)
    139 μl of Glycine pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H7
    • Glycine 0.2 M pH 9.2
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.2
    116 μl of Glycine pH 9.5 (1 M)
    83 μl of Glycine pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H8
    • Glycine 0.2 M pH 9.5
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.5
    200 μl of Glycine pH 9.5 (1 M)
    0 μl of Glycine pH 8 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H9
    • AMPD 0.2 M pH 8.7
    • Polyethylene glycol 4,000 20 w/v -
    		pH 8.7
    0 μl of AMPD pH 10 (1 M)
    200 μl of AMPD pH 8.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H10
    • AMPD 0.2 M pH 9
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9
    68 μl of AMPD pH 10 (1 M)
    131 μl of AMPD pH 8.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H11
    • AMPD 0.2 M pH 9.3
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.3
    127 μl of AMPD pH 10 (1 M)
    72 μl of AMPD pH 8.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)
    H12
    • AMPD 0.2 M pH 9.6
    • Polyethylene glycol 4,000 20 w/v -
    		pH 9.6
    168 μl of AMPD pH 10 (1 M)
    31 μl of AMPD pH 8.7 (1 M)
    200 μl of Polyethylene glycol 4,000 (100 w/v)
    600 μl of water (100 v/v)