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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
PEG pH HT
Barcodes
PEG pH HT
No barcodes found.
Screen Structure
PEG pH HT
Well Count | 96 |
---|---|
Number of distinct ingredients | 18 |
Internal diversity (score mean dist) | 0.631 |
Screen Composition
ingredient type | PEG pH HT |
---|---|
Buffer | 96 |
Polymer | 96 |
Salt | 28 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 3.5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 64.9 μl of tri-Sodium citrate pH 4.5 (1 M) 135.1 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A2 |
| pH 3.8 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 70.2 μl of tri-Sodium citrate pH 4.5 (1 M) 129.8 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A3 |
| pH 4.1 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 76.3 μl of tri-Sodium citrate pH 4.5 (1 M) 123.7 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A4 |
| pH 4.4 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 84.0 μl of tri-Sodium citrate pH 4.5 (1 M) 116.0 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A5 |
| pH 3.6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 66.4 μl of tri-Sodium citrate pH 4.5 (1 M) 133.6 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A6 |
| pH 3.9 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 71.9 μl of tri-Sodium citrate pH 4.5 (1 M) 128.1 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A7 |
| pH 4.2 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 78.7 μl of tri-Sodium citrate pH 4.5 (1 M) 121.3 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A8 |
| pH 4.5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 87.0 μl of tri-Sodium citrate pH 4.5 (1 M) 113.0 μl of tri-Sodium citrate pH 3.5 (1 M) 600.0 μl of water (100 v/v) |
A9 |
| pH 3.7 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 200.0 μl of Sodium acetate pH 3.7 (1 M) 0.0 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v) |
A10 |
| pH 4 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 176.6 μl of Sodium acetate pH 3.7 (1 M) 23.4 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v) |
A11 |
| pH 4.3 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 138.2 μl of Sodium acetate pH 3.7 (1 M) 61.8 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v) |
A12 |
| pH 4.6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 83.9 μl of Sodium acetate pH 3.7 (1 M) 116.1 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v) |
B1 |
| pH 4.9 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 20.7 μl of Sodium acetate pH 3.7 (1 M) 179.3 μl of Sodium acetate pH 5 (1 M) 600.0 μl of water (100 v/v) |
B2 |
| pH 4.7 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 25.7 μl of di-Sodium DL-malate pH 2.9 (3 M) 41.0 μl of di-Sodium DL-malate pH 7 (3 M) 733.3 μl of water (100 v/v) |
B3 |
| pH 5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 24.3 μl of di-Sodium DL-malate pH 2.9 (3 M) 42.4 μl of di-Sodium DL-malate pH 7 (3 M) 733.3 μl of water (100 v/v) |
B4 |
| pH 5.3 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 23.3 μl of di-Sodium DL-malate pH 2.9 (3 M) 43.3 μl of di-Sodium DL-malate pH 7 (3 M) 733.3 μl of water (100 v/v) |
B5 |
| pH 5.6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 22.8 μl of di-Sodium DL-malate pH 2.9 (3 M) 43.9 μl of di-Sodium DL-malate pH 7 (3 M) 733.3 μl of water (100 v/v) |
B6 |
| pH 5.9 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 22.5 μl of di-Sodium DL-malate pH 2.9 (3 M) 44.2 μl of di-Sodium DL-malate pH 7 (3 M) 733.3 μl of water (100 v/v) |
B7 |
| pH 4.8 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 184.5 μl of di-Sodium succinate pH 4.5 (1 M) 15.5 μl of di-Sodium succinate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
B8 |
| pH 5.1 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 159.1 μl of di-Sodium succinate pH 4.5 (1 M) 40.9 μl of di-Sodium succinate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
B9 |
| pH 5.4 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 123.1 μl of di-Sodium succinate pH 4.5 (1 M) 76.9 μl of di-Sodium succinate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
B10 |
| pH 5.7 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 81.2 μl of di-Sodium succinate pH 4.5 (1 M) 118.8 μl of di-Sodium succinate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
B11 |
| pH 6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 42.5 μl of di-Sodium succinate pH 4.5 (1 M) 157.5 μl of di-Sodium succinate pH 6.5 (1 M) 600.0 μl of water (100 v/v) |
B12 |
| pH 5.2 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 188.6 μl of Sodium cacodylate pH 5 (1 M) 11.4 μl of Sodium cacodylate pH 6.4 (1 M) 600.0 μl of water (100 v/v) |
C1 |
| pH 5.5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 161.4 μl of Sodium cacodylate pH 5 (1 M) 38.6 μl of Sodium cacodylate pH 6.4 (1 M) 600.0 μl of water (100 v/v) |
C2 |
| pH 5.8 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 118.6 μl of Sodium cacodylate pH 5 (1 M) 81.4 μl of Sodium cacodylate pH 6.4 (1 M) 600.0 μl of water (100 v/v) |
C3 |
| pH 6.1 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 61.6 μl of Sodium cacodylate pH 5 (1 M) 138.4 μl of Sodium cacodylate pH 6.4 (1 M) 600.0 μl of water (100 v/v) |
C4 |
| pH 6.4 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) -0.0 μl of Sodium cacodylate pH 5 (1 M) 200.0 μl of Sodium cacodylate pH 6.4 (1 M) 600.0 μl of water (100 v/v) |
C5 |
| pH 5.3 | 180.3 μl of MES pH 5 (1 M) 19.7 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C6 |
| pH 5.6 | 148.1 μl of MES pH 5 (1 M) 51.9 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C7 |
| pH 5.9 | 102.4 μl of MES pH 5 (1 M) 97.6 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C8 |
| pH 6.2 | 49.2 μl of MES pH 5 (1 M) 150.8 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C9 |
| pH 6.5 | 0.0 μl of MES pH 5 (1 M) 200.0 μl of MES pH 6.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C10 |
| pH 5.7 | 13.7 μl of BIS-TRIS pH 7 (1 M) 186.3 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C11 |
| pH 6 | 44.7 μl of BIS-TRIS pH 7 (1 M) 155.3 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
C12 |
| pH 6.3 | 88.5 μl of BIS-TRIS pH 7 (1 M) 111.5 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D1 |
| pH 6.6 | 139.5 μl of BIS-TRIS pH 7 (1 M) 60.5 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D2 |
| pH 6.9 | 186.7 μl of BIS-TRIS pH 7 (1 M) 13.3 μl of BIS-TRIS pH 5.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D3 |
| pH 5.8 | 180.3 μl of ADA pH 5.5 (1 M) 19.7 μl of ADA pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D4 |
| pH 6.1 | 148.1 μl of ADA pH 5.5 (1 M) 51.9 μl of ADA pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D5 |
| pH 6.4 | 102.4 μl of ADA pH 5.5 (1 M) 97.6 μl of ADA pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D6 |
| pH 6.7 | 49.2 μl of ADA pH 5.5 (1 M) 150.8 μl of ADA pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D7 |
| pH 7 | 0.0 μl of ADA pH 5.5 (1 M) 200.0 μl of ADA pH 7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D8 |
| pH 6.2 | 200.0 μl of Imidazole pH 6.2 (1 M) 0.0 μl of Imidazole pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D9 |
| pH 6.5 | 166.8 μl of Imidazole pH 6.2 (1 M) 33.2 μl of Imidazole pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D10 |
| pH 6.8 | 119.7 μl of Imidazole pH 6.2 (1 M) 80.3 μl of Imidazole pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D11 |
| pH 7.1 | 65.0 μl of Imidazole pH 6.2 (1 M) 135.0 μl of Imidazole pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
D12 |
| pH 7.4 | 14.3 μl of Imidazole pH 6.2 (1 M) 185.7 μl of Imidazole pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E1 |
| pH 6.4 | -0.0 μl of BIS-TRIS propane pH 7.5 (1 M) 200.0 μl of BIS-TRIS propane pH 6.4 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E2 |
| pH 6.7 | 57.5 μl of BIS-TRIS propane pH 7.5 (1 M) 142.5 μl of BIS-TRIS propane pH 6.4 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E3 |
| pH 7 | 119.7 μl of BIS-TRIS propane pH 7.5 (1 M) 80.3 μl of BIS-TRIS propane pH 6.4 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E4 |
| pH 7.3 | 173.1 μl of BIS-TRIS propane pH 7.5 (1 M) 26.9 μl of BIS-TRIS propane pH 6.4 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E5 |
| pH 6.5 | 200.0 μl of MOPS pH 6.5 (1 M) 0.0 μl of MOPS pH 7.9 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E6 |
| pH 6.8 | 164.5 μl of MOPS pH 6.5 (1 M) 35.5 μl of MOPS pH 7.9 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E7 |
| pH 7.1 | 117.2 μl of MOPS pH 6.5 (1 M) 82.8 μl of MOPS pH 7.9 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E8 |
| pH 7.4 | 66.1 μl of MOPS pH 6.5 (1 M) 133.9 μl of MOPS pH 7.9 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E9 |
| pH 7.7 | 22.2 μl of MOPS pH 6.5 (1 M) 177.8 μl of MOPS pH 7.9 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E10 |
| pH 6.6 | 200.0 μl of HEPES pH 6.6 (1 M) 0.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E11 |
| pH 6.9 | 154.2 μl of HEPES pH 6.6 (1 M) 45.8 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
E12 |
| pH 7.2 | 85.6 μl of HEPES pH 6.6 (1 M) 114.4 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F1 |
| pH 7.5 | -0.0 μl of HEPES pH 6.6 (1 M) 200.0 μl of HEPES pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F2 |
| pH 6.8 | 177.4 μl of HEPES pH 6.5 (1 M) 22.6 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F3 |
| pH 7.1 | 142.0 μl of HEPES pH 6.5 (1 M) 58.0 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F4 |
| pH 7.4 | 94.8 μl of HEPES pH 6.5 (1 M) 105.2 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F5 |
| pH 7.7 | 43.8 μl of HEPES pH 6.5 (1 M) 156.2 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
F6 |
| pH 7.2 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 188.1 μl of tris chloride BCC pH 7 (1 M) 11.9 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F7 |
| pH 7.5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 160.4 μl of tris chloride BCC pH 7 (1 M) 39.6 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F8 |
| pH 7.8 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 118.9 μl of tris chloride BCC pH 7 (1 M) 81.1 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F9 |
| pH 8.1 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 67.1 μl of tris chloride BCC pH 7 (1 M) 132.9 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F10 |
| pH 7.3 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 180.3 μl of tris chloride BCC pH 7 (1 M) 19.7 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F11 |
| pH 7.6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 148.1 μl of tris chloride BCC pH 7 (1 M) 51.9 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
F12 |
| pH 7.9 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 102.4 μl of tris chloride BCC pH 7 (1 M) 97.6 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
G1 |
| pH 8.2 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 49.2 μl of tris chloride BCC pH 7 (1 M) 150.8 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
G2 |
| pH 8.5 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 0.0 μl of tris chloride BCC pH 7 (1 M) 200.0 μl of tris chloride BCC pH 8.5 (1 M) 600.0 μl of water (100 v/v) |
G3 |
| pH 7.4 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 200.0 μl of TRICINE pH 7.4 (1 M) 0.0 μl of TRICINE pH 8.8 (1 M) 600.0 μl of water (100 v/v) |
G4 |
| pH 7.7 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 164.5 μl of TRICINE pH 7.4 (1 M) 35.5 μl of TRICINE pH 8.8 (1 M) 600.0 μl of water (100 v/v) |
G5 |
| pH 8 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 117.2 μl of TRICINE pH 7.4 (1 M) 82.8 μl of TRICINE pH 8.8 (1 M) 600.0 μl of water (100 v/v) |
G6 |
| pH 8.3 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 66.1 μl of TRICINE pH 7.4 (1 M) 133.9 μl of TRICINE pH 8.8 (1 M) 600.0 μl of water (100 v/v) |
G7 |
| pH 8.6 | 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 22.2 μl of TRICINE pH 7.4 (1 M) 177.8 μl of TRICINE pH 8.8 (1 M) 600.0 μl of water (100 v/v) |
G8 |
| pH 7.5 | 0.0 μl of BICINE pH 8.8 (1 M) 200.0 μl of BICINE pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
G9 |
| pH 7.8 | 33.2 μl of BICINE pH 8.8 (1 M) 166.8 μl of BICINE pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
G10 |
| pH 8.1 | 80.3 μl of BICINE pH 8.8 (1 M) 119.7 μl of BICINE pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
G11 |
| pH 8.4 | 135.0 μl of BICINE pH 8.8 (1 M) 65.0 μl of BICINE pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
G12 |
| pH 8.7 | 185.7 μl of BICINE pH 8.8 (1 M) 14.3 μl of BICINE pH 7.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H1 |
| pH 8.5 | 200.0 μl of BIS-TRIS propane pH 8.5 (1 M) 0.0 μl of BIS-TRIS propane pH 9.7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H2 |
| pH 8.8 | 94.4 μl of BIS-TRIS propane pH 8.5 (1 M) 105.6 μl of BIS-TRIS propane pH 9.7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H3 |
| pH 9.1 | 40.7 μl of BIS-TRIS propane pH 8.5 (1 M) 159.3 μl of BIS-TRIS propane pH 9.7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H4 |
| pH 9.4 | 13.6 μl of BIS-TRIS propane pH 8.5 (1 M) 186.4 μl of BIS-TRIS propane pH 9.7 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H5 |
| pH 8.6 | 172.5 μl of Glycine pH 8 (1 M) 27.5 μl of Glycine pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H6 |
| pH 8.9 | 139.5 μl of Glycine pH 8 (1 M) 60.5 μl of Glycine pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H7 |
| pH 9.2 | 83.6 μl of Glycine pH 8 (1 M) 116.4 μl of Glycine pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H8 |
| pH 9.5 | 0.0 μl of Glycine pH 8 (1 M) 200.0 μl of Glycine pH 9.5 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H9 |
| pH 8.7 | 200.0 μl of AMPD pH 8.7 (1 M) 0.0 μl of AMPD pH 10 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H10 |
| pH 9 | 131.5 μl of AMPD pH 8.7 (1 M) 68.5 μl of AMPD pH 10 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H11 |
| pH 9.3 | 72.7 μl of AMPD pH 8.7 (1 M) 127.3 μl of AMPD pH 10 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |
H12 |
| pH 9.6 | 31.1 μl of AMPD pH 8.7 (1 M) 168.9 μl of AMPD pH 10 (1 M) 200.0 μl of Polyethylene glycol 4,000 (100 w/v) 600.0 μl of water (100 v/v) |