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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
We hope you benefit from this website and encourage your feedback. If a feature does not behave as expected, content is missing, or you have recommendations to improve the user experience, please email bcc@csiro.au.
Supplemental Information
Screen Description
Export
BCS Eco
Barcodes
BCS Eco
No barcodes found.
Screen Structure
BCS Eco
Well Count | 96 |
---|---|
Number of distinct ingredients | 47 |
Internal diversity (score mean dist) | 0.927 |
Screen Composition
ingredient type | BCS Eco |
---|---|
Buffer | 117 |
Complex ingredient | 96 |
Cryoprotectant | 7 |
Organic (non-volatile) | 18 |
Organic (volatile) | 3 |
Salt | 118 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 4.5 | 600.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 300.0 μl of water (100 v/v) |
A2 |
| pH 5.5 | 600.0 μl of PEG Smear Low (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 300.0 μl of water (100 v/v) |
A3 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v) |
A4 |
| pH 4.5 | 500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 400.0 μl of water (100 v/v) |
A5 |
| pH 5.5 | 500.0 μl of PEG Smear Medium (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 400.0 μl of water (100 v/v) |
A6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v) |
A7 |
| pH 4.5 | 400.0 μl of PEG Smear High (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 500.0 μl of water (100 v/v) |
A8 |
| pH 5.5 | 400.0 μl of PEG Smear High (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 500.0 μl of water (100 v/v) |
A9 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v) |
A10 |
| pH 4.5 | 440.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 460.0 μl of water (100 v/v) |
A11 |
| pH 5.5 | 440.0 μl of PEG Smear Broad (50 v/v) 98.0 μl of Sodium phosphate citrate pH 6 (1 M) 2.0 μl of Sodium phosphate citrate pH 5 (1 M) 460.0 μl of water (100 v/v) |
A12 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v) |
B1 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v) |
B2 |
| pH 8.5 | 600.0 μl of PEG Smear Low (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 300.0 μl of water (100 v/v) |
B3 |
| pH 9.3 | 100.0 μl of BICINE pH 9.3 (1 M) 600.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v) |
B4 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v) |
B5 |
| pH 8.5 | 500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 400.0 μl of water (100 v/v) |
B6 |
| pH 9.3 | 100.0 μl of BICINE pH 9.3 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 400.0 μl of water (100 v/v) |
B7 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v) |
B8 |
| pH 8.5 | 400.0 μl of PEG Smear High (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 500.0 μl of water (100 v/v) |
B9 |
| pH 9.3 | 100.0 μl of BICINE pH 9.3 (1 M) 400.0 μl of PEG Smear High (50 v/v) 500.0 μl of water (100 v/v) |
B10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v) |
B11 |
| pH 8.5 | 440.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 460.0 μl of water (100 v/v) |
B12 |
| pH 9.3 | 100.0 μl of BICINE pH 9.3 (1 M) 440.0 μl of PEG Smear Broad (50 v/v) 460.0 μl of water (100 v/v) |
C1 |
| NA | 700.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v) |
C2 |
| pH 4.6 | 66.7 μl of Ammonium acetate (3 M) 166.7 μl of Ethylene glycol (30 v/v) 560.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 106.7 μl of water (100 v/v) |
C3 |
| NA | 560.0 μl of PEG Smear Medium (50 v/v) 75.0 μl of Sodium chloride (2 M) 365.0 μl of water (100 v/v) |
C4 |
| pH 5.5 | 100.0 μl of Ammonium sulfate (2 M) 100.0 μl of MES pH 5.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 300.0 μl of water (100 v/v) |
C5 |
| NA | 500.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Rubidium chloride (1 M) 100.0 μl of Sodium Potassium phosphate pH 5.5 (1 M) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 200.0 μl of water (100 v/v) |
C6 |
| NA | 450.0 μl of PEG Smear High (50 v/v) 200.0 μl of Potassium chloride (1 M) 350.0 μl of water (100 v/v) |
C7 |
| pH 5 | 150.0 μl of Ammonium acetate (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 450.0 μl of water (100 v/v) |
C8 |
| NA | 166.7 μl of Glycerol (30 v/v) 50.0 μl of L-Arginine (1 M) 50.0 μl of L-Glutamic acid (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 173.3 μl of water (100 v/v) |
C9 |
| pH 5.6 | 150.0 μl of Magnesium acetate (1 M) 400.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.6 (1 M) 350.0 μl of water (100 v/v) |
C10 |
| pH 4.6 | 200.0 μl of Ammonium sulfate (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Sodium acetate pH 4.6 (1 M) 200.0 μl of water (100 v/v) |
C11 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 500.0 μl of PEG Smear Low (50 v/v) 200.0 μl of Potassium Sodium tartrate (1 M) 200.0 μl of water (100 v/v) |
C12 |
| pH 7 | 50.0 μl of Calcium chloride (2 M) 100.0 μl of Magnesium chloride (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 300.0 μl of water (100 v/v) |
D1 |
| pH 5.3 | 100.0 μl of Ammonium nitrate (2 M) 50.3 μl of MES pH 5 (1 M) 49.7 μl of MES pH 5.5 (1 M) 450.0 μl of PEG Smear Low (50 v/v) 350.0 μl of water (100 v/v) |
D2 |
| pH 6.5 | 333.3 μl of 2-Propanol (30 v/v) 100.0 μl of MES pH 6.5 (1 M) 450.0 μl of PEG Smear Low (50 v/v) 116.7 μl of water (100 v/v) |
D3 |
| pH 6 | 75.0 μl of Ammonium nitrate (2 M) 166.7 μl of Ethylene glycol (30 v/v) 100.0 μl of MES pH 6 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 258.3 μl of water (100 v/v) |
D4 |
| pH 6.2 | 200.0 μl of Glycerol (50 v/v) 400.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium Phosphate pH 6.2 (1 M) 50.0 μl of Sodium formate (4 M) 250.0 μl of water (100 v/v) |
D5 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 100.0 μl of Lithium sulfate (2 M) 600.0 μl of PEG Smear Medium (50 v/v) 200.0 μl of water (100 v/v) |
D6 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 240.0 μl of PEG Smear High (50 v/v) 12.5 μl of Potassium thiocyanate (8 M) 20.0 μl of Sodium bromide (5 M) 627.5 μl of water (100 v/v) |
D7 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 50.0 μl of Ammonium sulfate (4 M) 360.0 μl of PEG Smear High (50 v/v) 490.0 μl of water (100 v/v) |
D8 |
| pH 6.2 | 75.0 μl of Calcium chloride (2 M) 166.7 μl of Glycerol (30 v/v) 100.0 μl of MES pH 6.2 (1 M) 300.0 μl of PEG Smear Broad (50 v/v) 358.3 μl of water (100 v/v) |
D9 |
| pH 5.3 | 200.0 μl of Ethylene glycol (50 w/v) 50.3 μl of MES pH 5 (1 M) 49.7 μl of MES pH 5.5 (1 M) 300.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of T-Mate (100 v/v) 350.0 μl of water (100 v/v) |
D10 |
| pH 6.2 | 560.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Sodium Phosphate pH 6.2 (1 M) 200.0 μl of Sodium chloride (1 M) 140.0 μl of water (100 v/v) |
D11 |
| pH 5.5 | 100.0 μl of Ammonium sulfate (1 M) 50.0 μl of Magnesium sulfate (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 300.0 μl of water (100 v/v) |
D12 |
| pH 8 | 72.9 μl of BIS-TRIS propane pH 8.5 (1 M) 27.1 μl of BIS-TRIS propane pH 7.5 (1 M) 10.0 μl of Cobalt (II) chloride (1 M) 66.7 μl of Glycerol (30 v/v) 100.0 μl of Magnesium chloride (2 M) 450.0 μl of PEG Smear Medium (50 v/v) 273.3 μl of water (100 v/v) |
E1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 80.0 μl of Magnesium acetate (1 M) 20.0 μl of Magnesium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 300.0 μl of water (100 v/v) |
E2 |
| pH 7.5 | 166.7 μl of Ethylene glycol (30 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of PEG Smear Low (50 v/v) 100.0 μl of Potassium chloride (1 M) 313.3 μl of water (100 v/v) |
E3 |
| pH 7.5 | 100.0 μl of BIS-TRIS pH 7.5 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Zinc acetate (1 M) 100.0 μl of Zinc chloride (1 M) 300.0 μl of water (100 v/v) |
E4 |
| pH 7 | 20.0 μl of Magnesium chloride (5 M) 400.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Potassium chloride (1 M) 380.0 μl of water (100 v/v) |
E5 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Magnesium sulfate (1 M) 560.0 μl of PEG Smear Medium (50 v/v) 290.0 μl of water (100 v/v) |
E6 |
| NA | 333.3 μl of Ethylene glycol (30 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of Sodium Potassium phosphate pH 7.5 (1 M) 166.7 μl of water (100 v/v) |
E7 |
| pH 7 | 100.0 μl of Magnesium formate (1 M) 500.0 μl of PEG Smear High (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Rubidium chloride (1 M) 200.0 μl of water (100 v/v) |
E8 |
| pH 7.2 | 36.0 μl of HEPES pH 7.5 (1 M) 64.0 μl of HEPES pH 7 (1 M) 100.0 μl of Lithium sulfate (2 M) 500.0 μl of PEG Smear Broad (50 v/v) 300.0 μl of water (100 v/v) |
E9 |
| pH 7.5 | 100.0 μl of Ammonium nitrate (2 M) 100.0 μl of HEPES pH 7.5 (1 M) 400.0 μl of PEG Smear Broad (50 v/v) 400.0 μl of water (100 v/v) |
E10 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 50.0 μl of Magnesium chloride (2 M) 600.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Rubidium chloride (1 M) 150.0 μl of water (100 v/v) |
E11 |
| pH 7.8 | 51.4 μl of BIS-TRIS propane pH 8.5 (1 M) 48.6 μl of BIS-TRIS propane pH 7.5 (1 M) 50.0 μl of Magnesium chloride (1 M) 450.0 μl of PEG Smear High (50 v/v) 50.0 μl of tri-Sodium citrate (1 M) 350.0 μl of water (100 v/v) |
E12 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 333.3 μl of Ethylene glycol (30 v/v) 450.0 μl of PEG Smear High (50 v/v) 70.0 μl of T-Mate (100 v/v) 46.7 μl of water (100 v/v) |
F1 |
| pH 7.8 | 100.0 μl of HEPES pH 7.8 (1 M) 500.0 μl of PEG Smear Low (50 v/v) 150.0 μl of tri-Sodium citrate (1 M) 250.0 μl of water (100 v/v) |
F2 |
| pH 8.5 | 166.7 μl of Glycerol (30 v/v) 560.0 μl of PEG Smear Low (50 v/v) 40.0 μl of Sodium chloride (5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 133.3 μl of water (100 v/v) |
F3 |
| pH 8 | 300.0 μl of PEG Smear Medium (50 v/v) 40.0 μl of Sodium acetate (2 M) 75.0 μl of Sodium chloride (2 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 485.0 μl of water (100 v/v) |
F4 |
| pH 8.5 | 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 50.0 μl of Sodium chloride (2 M) 25.0 μl of Sodium formate (4 M) 325.0 μl of water (100 v/v) |
F5 |
| pH 9 | 200.0 μl of Ammonium sulfate (1 M) 100.0 μl of BICINE pH 9 (1 M) 50.0 μl of Magnesium sulfate (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 250.0 μl of water (100 v/v) |
F6 |
| pH 8.5 | 100.0 μl of Ammonium nitrate (2 M) 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 360.0 μl of PEG Smear High (50 v/v) 440.0 μl of water (100 v/v) |
F7 |
| pH 8 | 100.0 μl of Glycerol (100 v/v) 100.0 μl of Magnesium chloride (2 M) 500.0 μl of PEG Smear High (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 200.0 μl of water (100 v/v) |
F8 |
| pH 8.5 | 150.0 μl of Ammonium acetate (1 M) 10.0 μl of Calcium chloride (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 180.0 μl of water (100 v/v) |
F9 |
| pH 9 | 100.0 μl of 2-Propanol (100 v/v) 100.0 μl of BICINE pH 9 (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 300.0 μl of water (100 v/v) |
F10 |
| pH 8 | 100.0 μl of Ammonium sulfate (2 M) 400.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 400.0 μl of water (100 v/v) |
F11 |
| pH 8.8 | 100.0 μl of BICINE pH 8.8 (1 M) 20.0 μl of Magnesium sulfate (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 200.0 μl of Potassium chloride (1 M) 230.0 μl of water (100 v/v) |
F12 |
| pH 5.5 | 100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 5.5 (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of Potassium Sodium tartrate (1 M) 250.0 μl of water (100 v/v) |
G1 |
| pH 6.2 | 10.0 μl of Cobalt (II) chloride (1 M) 100.0 μl of MES pH 6.2 (1 M) 100.0 μl of Magnesium formate (1 M) 280.0 μl of PEG Smear Low (50 v/v) 510.0 μl of water (100 v/v) |
G2 |
| pH 6.8 | 100.0 μl of BIS-TRIS pH 6.8 (1 M) 150.0 μl of Lithium sulfate (1 M) 50.0 μl of Magnesium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 200.0 μl of water (100 v/v) |
G3 |
| pH 7.5 | 200.0 μl of Ammonium sulfate (1 M) 10.0 μl of Cadmium chloride (1 M) 100.0 μl of HEPES pH 7.5 (1 M) 500.0 μl of PEG Smear Medium (50 v/v) 190.0 μl of water (100 v/v) |
G4 |
| NA | 100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of Magnesium chloride (1 M) 360.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Potassium chloride (1 M) 340.0 μl of water (100 v/v) |
G5 |
| pH 6.5 | 100.0 μl of Ethylene glycol (100 v/v) 100.0 μl of MES pH 6.5 (1 M) 100.0 μl of Magnesium acetate (1 M) 240.0 μl of PEG Smear Medium (50 v/v) 460.0 μl of water (100 v/v) |
G6 |
| pH 6.2 | 100.0 μl of MES pH 6.2 (1 M) 100.0 μl of Magnesium acetate (1 M) 240.0 μl of PEG Smear High (50 v/v) 100.0 μl of Potassium chloride (1 M) 460.0 μl of water (100 v/v) |
G7 |
| pH 7 | 400.0 μl of Calcium chloride (0.1 M) 160.0 μl of PEG Smear High (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 10.0 μl of Sodium formate (4 M) 330.0 μl of water (100 v/v) |
G8 |
| pH 6 | 100.0 μl of BIS-TRIS pH 6 (1 M) 80.0 μl of Magnesium chloride (1 M) 360.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of tri-Sodium citrate (1.6 M) 410.0 μl of water (100 v/v) |
G9 |
| pH 6.5 | 100.0 μl of BIS-TRIS pH 6.5 (1 M) 100.0 μl of Magnesium chloride (1 M) 300.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of Sodium acetate (2 M) 450.0 μl of water (100 v/v) |
G10 |
| pH 7 | 100.0 μl of Ammonium sulfate (1 M) 100.0 μl of HEPES pH 7 (1 M) 500.0 μl of PEG Smear Broad (50 v/v) 25.0 μl of Sodium formate (4 M) 275.0 μl of water (100 v/v) |
G11 |
| NA | 100.0 μl of Glycerol (100 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 450.0 μl of PEG Smear Medium (50 v/v) 100.0 μl of Sodium Potassium phosphate pH 7.5 (2 M) 250.0 μl of water (100 v/v) |
G12 |
| pH 7.5 | 50.0 μl of L-Arginine (1 M) 50.0 μl of L-Glutamic acid (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 150.0 μl of Sodium chloride (2 M) 100.0 μl of tris chloride BCC pH 7.5 (1 M) 200.0 μl of water (100 v/v) |
H1 |
| pH 8 | 40.0 μl of Calcium chloride (1 M) 500.0 μl of PEG Smear Low (50 v/v) 10.0 μl of Sodium formate (4 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 350.0 μl of water (100 v/v) |
H2 |
| pH 7 | 100.0 μl of Magnesium chloride (1 M) 400.0 μl of PEG Smear Low (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 100.0 μl of Rubidium chloride (1 M) 300.0 μl of water (100 v/v) |
H3 |
| pH 7.5 | 50.0 μl of 2-Propanol (100 v/v) 200.0 μl of Ethylene glycol (50 v/v) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Magnesium chloride (2 M) 300.0 μl of PEG Smear Medium (50 v/v) 250.0 μl of water (100 v/v) |
H4 |
| pH 7 | 50.0 μl of Ammonium acetate (1 M) 100.0 μl of HEPES pH 7 (1 M) 150.0 μl of Magnesium sulfate (1 M) 240.0 μl of PEG Smear Medium (50 v/v) 460.0 μl of water (100 v/v) |
H5 |
| pH 7.2 | 51.9 μl of HEPES pH 7.1 (1 M) 48.1 μl of HEPES pH 7.3 (1 M) 400.0 μl of PEG Smear Medium (50 v/v) 70.0 μl of T-Mate (100 v/v) 430.0 μl of water (100 v/v) |
H6 |
| pH 7.2 | 100.0 μl of Ammonium acetate (1 M) 50.5 μl of BIS-TRIS pH 7 (1 M) 49.5 μl of BIS-TRIS pH 7.5 (1 M) 300.0 μl of PEG Smear High (50 v/v) 100.0 μl of Zinc chloride (1 M) 400.0 μl of water (100 v/v) |
H7 |
| pH 7.8 | 100.0 μl of HEPES pH 7.8 (1 M) 150.0 μl of Lithium sulfate (1 M) 50.0 μl of Magnesium chloride (1 M) 400.0 μl of PEG Smear High (50 v/v) 300.0 μl of water (100 v/v) |
H8 |
| pH 7.8 | 500.0 μl of PEG Smear Broad (50 v/v) 200.0 μl of Potassium thiocyanate (500 mM) 20.0 μl of Sodium bromide (5 M) 74.1 μl of tris chloride BCC pH 7.5 (1 M) 25.9 μl of tris chloride BCC pH 8.5 (1 M) 180.0 μl of water (100 v/v) |
H9 |
| pH 8.5 | 50.0 μl of Ammonium sulfate (1 M) 100.0 μl of BIS-TRIS propane pH 8.5 (1 M) 50.0 μl of Lithium sulfate (1 M) 560.0 μl of PEG Smear Broad (50 v/v) 240.0 μl of water (100 v/v) |
H10 |
| pH 7 | 200.0 μl of Ammonium sulfate (1 M) 100.0 μl of Cadmium chloride (0.1 M) 100.0 μl of Ethylene glycol (100 v/v) 300.0 μl of PEG Smear Broad (50 v/v) 100.0 μl of PIPES pH 7 (1 M) 200.0 μl of water (100 v/v) |
H11 |
| pH 7.5 | 100.0 μl of BIS-TRIS pH 7.5 (1 M) 200.0 μl of Lithium sulfate (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 50.0 μl of Zinc acetate (1 M) 200.0 μl of water (100 v/v) |
H12 |
| pH 7.8 | 100.0 μl of HEPES pH 7.8 (1 M) 450.0 μl of PEG Smear Broad (50 v/v) 16.0 μl of Sodium bromide (5 M) 25.0 μl of Sodium fluoride (2 M) 160.0 μl of Sodium iodide (500 mM) 249.0 μl of water (100 v/v) |