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Supplemental Information

Screen Description

MemGoldMeso Eco

Molecular Dimensions / MemGoldMeso Eco
Created 2020-05-11.

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MemGoldMeso Eco

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Screen Structure

MemGoldMeso Eco

Well Count 96
Number of distinct ingredients 67
Internal diversity (score mean dist) 0.917

Screen Composition

2025-08-16T09:05:40.186130 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemGoldMeso Eco
Buffer 126
Organic (non-volatile) 54
Organic (volatile) 1
Polymer 77
Salt 130

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemGoldMeso Eco Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Sodium acetate 0.175 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 42 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
420.0 μl of Polyethylene glycol 400 (100 v/v)
87.5 μl of Sodium acetate (2 M)
392.5 μl of water (100 v/v)
A2
  • Sodium chloride 0.1 M -
  • Lithium nitrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 4 v/v -
pH 5.5
133.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
12.5 μl of Lithium nitrate (8 M)
50.0 μl of Sodium chloride (2 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
704.2 μl of water (100 v/v)
A3
  • Lithium sulfate 0.2 M -
  • MES 0.1 M pH 6.8
  • Polyethylene glycol 400 25 v/v -
pH 6.8
100.0 μl of Lithium sulfate (2 M)
31.5 μl of MES pH 6.5 (1 M)
68.5 μl of MES pH 7 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
550.0 μl of water (100 v/v)
A4
  • di-Sodium malonate 0.075 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 40 v/v -
pH 6
100.0 μl of MES pH 6 (1 M)
400.0 μl of Polyethylene glycol 300 (100 v/v)
37.5 μl of di-Sodium malonate (2 M)
462.5 μl of water (100 v/v)
A5
  • Lithium chloride 0.4 M -
  • tris chloride BCC 0.1 M pH 7.2
  • Polyethylene glycol 400 30 v/v -
pH 7.2
80.0 μl of Lithium chloride (5 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
77.1 μl of tris chloride BCC pH 7 (1 M)
22.9 μl of tris chloride BCC pH 7.6 (1 M)
520.0 μl of water (100 v/v)
A6
  • Sodium chloride 0.075 M -
  • BICINE 0.1 M pH 9
  • Polyethylene glycol 600 32 v/v -
pH 9
100.0 μl of BICINE pH 9 (1 M)
426.7 μl of Polyethylene glycol 600 (75 v/v)
37.5 μl of Sodium chloride (2 M)
435.8 μl of water (100 v/v)
A7
  • di-Sodium succinate 0.1 M -
  • MES 0.1 M pH 6.3
  • Polyethylene glycol 300 32 v/v -
pH 6.3
37.5 μl of MES pH 6 (1 M)
62.5 μl of MES pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 300 (100 v/v)
83.3 μl of di-Sodium succinate (1.2 M)
496.7 μl of water (100 v/v)
A8
  • Sodium chloride 0.1 M -
  • Magnesium acetate 0.06 M -
  • tri-Sodium citrate 0.05 M pH 6.3
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 6.3
166.7 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
60.0 μl of Magnesium acetate (1 M)
50.0 μl of Sodium chloride (2 M)
43.1 μl of tri-Sodium citrate pH 6.5 (1 M)
6.9 μl of tri-Sodium citrate pH 5.8 (1 M)
673.3 μl of water (100 v/v)
A9
  • Lithium sulfate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • Polyethylene glycol 400 30 v/v -
pH 5.5
100.0 μl of Lithium sulfate (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
A10
  • Magnesium sulfate 0.1 M -
  • Sodium sulfate 0.1 M -
  • Polyethylene glycol monomethyl ether 500 31 v/v -
  • MOPS 0.05 M pH 6
pH 6
50.0 μl of MOPS pH 6 (1 M)
50.0 μl of Magnesium sulfate (2 M)
310.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v)
100.0 μl of Sodium sulfate (1 M)
490.0 μl of water (100 v/v)
A11
  • Sodium chloride 0.15 M -
  • Sodium Potassium phosphate 0.9 M pH 5
pH 5
900.0 μl of Sodium Potassium phosphate pH 5 (1 M)
30.0 μl of Sodium chloride (5 M)
70.0 μl of water (100 v/v)
A12
  • Magnesium chloride 0.35 M -
  • tri-Sodium citrate 0.15 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 33 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
175.0 μl of Magnesium chloride (2 M)
330.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tri-Sodium citrate (3 M)
345.0 μl of water (100 v/v)
B1
  • Sodium Potassium phosphate 1 M pH 5
  • D-(+)-Trehalose 0.3 M -
pH 5
300.0 μl of D-(+)-Trehalose (1 M)
333.3 μl of Sodium Potassium phosphate pH 5 (3 M)
366.7 μl of water (100 v/v)
B2
  • Lithium sulfate 0.22 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol dimethyl ether 500 28 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
110.0 μl of Lithium sulfate (2 M)
280.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
510.0 μl of water (100 v/v)
B3
  • Ammonium fluoride 0.07 M -
  • HEPES 0.1 M pH 8
  • Polyethylene glycol 400 32 v/v -
pH 8
7.0 μl of Ammonium fluoride (10 M)
100.0 μl of HEPES pH 8 (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
573.0 μl of water (100 v/v)
B4
  • Lithium sulfate 0.2 M -
  • Sodium phosphate citrate 0.1 M pH 4.2
  • Polyethylene glycol 1,000 10 w/v -
pH 4.2
100.0 μl of Lithium sulfate (2 M)
200.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of Sodium phosphate citrate pH 4.2 (1 M)
600.0 μl of water (100 v/v)
B5
  • di-Ammonium hydrogen phosphate 0.3 M -
  • Magnesium chloride 0.01 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 400 30 v/v -
pH 4.5
100.0 μl of Magnesium chloride (0.1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
85.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
414.3 μl of water (100 v/v)
B6
  • Ammonium acetate 0.3 M -
  • BIS-TRIS 0.1 M pH 7.6
  • Polyethylene glycol 400 30 v/v -
pH 7.6
300.0 μl of Ammonium acetate (1 M)
20.5 μl of BIS-TRIS pH 7 (1 M)
79.5 μl of BIS-TRIS pH 8 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
300.0 μl of water (100 v/v)
B7
  • tri-Sodium citrate 0.1 M pH 3.6
  • Polyethylene glycol 400 34 v/v -
pH 3.6
340.0 μl of Polyethylene glycol 400 (100 v/v)
66.8 μl of tri-Sodium citrate pH 3.2 (1 M)
33.2 μl of tri-Sodium citrate pH 4 (1 M)
560.0 μl of water (100 v/v)
B8
  • Ammonium sulfate 0.56 M -
  • HEPES 0.08 M pH 7.5
  • Jeffamine® M-600 18 v/v -
pH 7.5
280.0 μl of Ammonium sulfate (2 M)
80.0 μl of HEPES pH 7.5 (1 M)
360.0 μl of Jeffamine® M-600 (50 v/v)
280.0 μl of water (100 v/v)
B9
  • Sodium formate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 27 v/v -
pH 8
270.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium formate (5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
590.0 μl of water (100 v/v)
B10
  • BIS-TRIS 0.1 M pH 5.5
  • Polyethylene glycol 3,350 25 w/v -
pH 5.5
100.0 μl of BIS-TRIS pH 5.5 (1 M)
500.0 μl of Polyethylene glycol 3,350 (50 w/v)
400.0 μl of water (100 v/v)
B11
  • Sodium acetate 0.05 M pH 5
  • penta-Ethylene glycol 22 v/v -
pH 5
50.0 μl of Sodium acetate pH 5 (1 M)
220.0 μl of penta-Ethylene glycol (100 v/v)
730.0 μl of water (100 v/v)
B12
  • Potassium Sodium tartrate 0.18 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 17 v/v -
pH 6
100.0 μl of MES pH 6 (1 M)
340.0 μl of Polyethylene glycol 400 (50 v/v)
120.0 μl of Potassium Sodium tartrate (1.5 M)
440.0 μl of water (100 v/v)
C1
  • Potassium nitrate 0.45 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol 400 30 v/v -
pH 6
300.0 μl of Polyethylene glycol 400 (100 v/v)
225.0 μl of Potassium nitrate (2 M)
22.5 μl of tri-Sodium citrate pH 5.8 (1 M)
77.5 μl of tri-Sodium citrate pH 6.2 (1 M)
375.0 μl of water (100 v/v)
C2
  • Magnesium chloride 0.04 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 400 30 v/v -
pH 8.5
20.0 μl of Magnesium chloride (2 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
C3
  • Sodium acetate 0.1 M -
  • Ammonium fluoride 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
pH 6
10.0 μl of Ammonium fluoride (10 M)
100.0 μl of MES pH 6 (1 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
33.3 μl of Sodium acetate (3 M)
556.7 μl of water (100 v/v)
C4
  • di-Ammonium hydrogen phosphate 0.1 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 300 45 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
450.0 μl of Polyethylene glycol 300 (100 v/v)
28.6 μl of di-Ammonium hydrogen phosphate (3.5 M)
421.4 μl of water (100 v/v)
C5
  • Sodium Potassium phosphate 1.1 M pH 5.1
pH 5.1
333.3 μl of Sodium Potassium phosphate pH 5 (3 M)
33.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)
633.3 μl of water (100 v/v)
C6
  • Ammonium sulfate 0.15 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 400 10 v/v -
pH 7.5
50.0 μl of Ammonium sulfate (3 M)
100.0 μl of HEPES pH 7.5 (1 M)
100.0 μl of Polyethylene glycol 400 (100 v/v)
750.0 μl of water (100 v/v)
C7
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol monomethyl ether 350 24 v/v -
pH 8.5
240.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of Sodium chloride (2 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
610.0 μl of water (100 v/v)
C8
  • di-Ammonium hydrogen phosphate 0.3 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 28 v/v -
pH 8
280.0 μl of Polyethylene glycol 400 (100 v/v)
85.7 μl of di-Ammonium hydrogen phosphate (3.5 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
534.3 μl of water (100 v/v)
C9
  • Sodium Potassium phosphate 0.1 M pH 5.6
  • Polyethylene glycol 2,000 32 w/v -
pH 5.6
533.3 μl of Polyethylene glycol 2,000 (60 w/v)
81.5 μl of Sodium Potassium phosphate pH 5 (1 M)
18.5 μl of Sodium Potassium phosphate pH 6 (1 M)
366.7 μl of water (100 v/v)
C10
  • Ammonium acetate 0.35 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 35 v/v -
pH 5
116.7 μl of Ammonium acetate (3 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
433.3 μl of water (100 v/v)
C11
  • Lithium sulfate 0.3 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 35 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
150.0 μl of Lithium sulfate (2 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
C12
  • Ammonium chloride 0.1 M -
  • Calcium chloride 0.01 M -
  • MES 0.1 M pH 6.5
  • Pentaerythritol Propoxylate (5/4 PO/OH) 10 v/v -
pH 6.5
20.0 μl of Ammonium chloride (5 M)
10.0 μl of Calcium chloride (1 M)
100.0 μl of MES pH 6.5 (1 M)
200.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
670.0 μl of water (100 v/v)
D1
  • di-Ammonium hydrogen phosphate 0.25 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 28 v/v -
pH 6
100.0 μl of MES pH 6 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
71.4 μl of di-Ammonium hydrogen phosphate (3.5 M)
548.6 μl of water (100 v/v)
D2
  • tri-Sodium citrate 0.08 M -
  • TRICINE 0.1 M pH 8.3
  • Polyethylene glycol 400 36 v/v -
pH 8.3
360.0 μl of Polyethylene glycol 400 (100 v/v)
62.5 μl of TRICINE pH 8.5 (1 M)
37.5 μl of TRICINE pH 8 (1 M)
50.0 μl of tri-Sodium citrate (1.6 M)
490.0 μl of water (100 v/v)
D3
  • Magnesium chloride 0.1 M -
  • tri-Sodium citrate 0.05 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 5 v/v -
pH 5.5
333.3 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
100.0 μl of Magnesium chloride (1 M)
50.0 μl of tri-Sodium citrate pH 5.5 (1 M)
516.7 μl of water (100 v/v)
D4
  • Sodium chloride 0.4 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 400 20 v/v -
pH 7
100.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
80.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
D5
  • Potassium formate 0.3 M -
  • BIS-TRIS 0.1 M pH 7
  • Polyethylene glycol 400 28 v/v -
pH 7
100.0 μl of BIS-TRIS pH 7 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
21.4 μl of Potassium formate (14 M)
598.6 μl of water (100 v/v)
D6
  • Sodium formate 0.2 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol 400 31 v/v -
pH 6
310.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium formate (4 M)
22.5 μl of tri-Sodium citrate pH 5.8 (1 M)
77.5 μl of tri-Sodium citrate pH 6.5 (1 M)
540.0 μl of water (100 v/v)
D7
  • Magnesium sulfate 0.35 M -
  • tris chloride BCC 0.1 M pH 8.3
  • Polyethylene glycol monomethyl ether 550 28 v/v -
pH 8.3
175.0 μl of Magnesium sulfate (2 M)
280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
61.7 μl of tris chloride BCC pH 8 (1 M)
38.3 μl of tris chloride BCC pH 9 (1 M)
445.0 μl of water (100 v/v)
D8
  • Potassium Sodium tartrate 0.1 M -
  • tris chloride BCC 0.1 M pH 8.8
  • Polyethylene glycol monomethyl ether 550 30 v/v -
  • Potassium nitrate 0.1 M -
pH 8.8
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
66.7 μl of Potassium Sodium tartrate (1.5 M)
100.0 μl of Potassium nitrate (1 M)
12.2 μl of tris chloride BCC pH 8 (1 M)
87.8 μl of tris chloride BCC pH 9 (1 M)
433.3 μl of water (100 v/v)
D9
  • BICINE 0.1 M pH 9
  • Ammonium sulfate 2 M -
pH 9
500.0 μl of Ammonium sulfate (4 M)
100.0 μl of BICINE pH 9 (1 M)
400.0 μl of water (100 v/v)
D10
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 4.5
  • Polyethylene glycol 400 18 v/v -
pH 4.5
50.0 μl of Lithium sulfate (2 M)
180.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of Sodium chloride (3 M)
100.0 μl of tri-Sodium citrate pH 4.5 (1 M)
636.7 μl of water (100 v/v)
D11
  • Sodium chloride 0.1 M -
  • Potassium nitrate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 5.5
  • 2-methyl-2,4-pentanediol BCC 8 v/v -
pH 5.5
266.7 μl of 2-methyl-2,4-pentanediol BCC (30 v/v)
100.0 μl of Potassium nitrate (1 M)
33.3 μl of Sodium chloride (3 M)
100.0 μl of tri-Sodium citrate pH 5.5 (1 M)
500.0 μl of water (100 v/v)
D12
  • ADA 0.05 M pH 5.8
  • Potassium Sodium tartrate 0.6 M -
pH 5.8
25.1 μl of ADA pH 5.5 (1 M)
24.9 μl of ADA pH 6 (1 M)
400.0 μl of Potassium Sodium tartrate (1.5 M)
550.0 μl of water (100 v/v)
E1
  • Ammonium formate 0.35 M -
  • tris chloride BCC 0.1 M pH 8.5
  • 1,4-Butanediol 18 v/v -
pH 8.5
450.0 μl of 1,4-Butanediol (40 v/v)
140.0 μl of Ammonium formate (2.5 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
310.0 μl of water (100 v/v)
E2
  • Ethylenediaminetetraacetic acid disodium salt 0.075 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 300 25 v/v -
pH 6
150.0 μl of Ethylenediaminetetraacetic acid disodium salt (0.5 M)
100.0 μl of MES pH 6 (1 M)
250.0 μl of Polyethylene glycol 300 (100 v/v)
500.0 μl of water (100 v/v)
E3
  • tri-Ammonium citrate 0.05 M -
  • HEPES 0.1 M pH 6.8
  • Polyethylene glycol 400 28 v/v -
pH 6.8
57.5 μl of HEPES pH 6.6 (1 M)
42.5 μl of HEPES pH 7 (1 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of tri-Ammonium citrate (2.5 M)
600.0 μl of water (100 v/v)
E4
  • di-Sodium malonate 0.2 M -
  • Sodium acetate 0.1 M pH 4.5
  • Polyethylene glycol 3,350 12 w/v -
pH 4.5
240.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of Sodium acetate pH 4.5 (1 M)
100.0 μl of di-Sodium malonate (2 M)
560.0 μl of water (100 v/v)
E5
  • Zinc sulfate 0.025 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol monomethyl ether 550 27 v/v -
pH 5
270.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
25.0 μl of Zinc sulfate (1 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
605.0 μl of water (100 v/v)
E6
  • tris chloride BCC 0.1 M pH 7.5
  • Polyethylene glycol 400 14 v/v -
pH 7.5
280.0 μl of Polyethylene glycol 400 (50 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
620.0 μl of water (100 v/v)
E7
  • Ammonium nitrate 0.1 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol dimethyl ether 500 27 v/v -
pH 6.5
50.0 μl of Ammonium nitrate (2 M)
100.0 μl of MES pH 6.5 (1 M)
270.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
580.0 μl of water (100 v/v)
E8
  • di-Ammonium hydrogen phosphate 0.2 M -
  • HEPES 0.1 M pH 6.8
  • Polyethylene glycol 400 24 v/v -
pH 6.8
57.5 μl of HEPES pH 6.6 (1 M)
42.5 μl of HEPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 400 (100 v/v)
57.1 μl of di-Ammonium hydrogen phosphate (3.5 M)
602.9 μl of water (100 v/v)
E9
  • Ammonium di-hydrogen phosphate 0.4 M -
  • ADA 0.1 M pH 7
  • Polyethylene glycol 400 34 v/v -
pH 7
100.0 μl of ADA pH 7 (1 M)
400.0 μl of Ammonium di-hydrogen phosphate (1 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
160.0 μl of water (100 v/v)
E10
  • Magnesium Citrate 0.05 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol 200 42 v/v -
pH 4
50.0 μl of Magnesium Citrate (1 M)
420.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of Sodium acetate pH 4 (1 M)
430.0 μl of water (100 v/v)
E11
  • tri-Potassium citrate 0.4 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol 400 28 v/v -
pH 6
280.0 μl of Polyethylene glycol 400 (100 v/v)
266.7 μl of tri-Potassium citrate (1.5 M)
77.5 μl of tri-Sodium citrate pH 6.2 (1 M)
22.5 μl of tri-Sodium citrate pH 5.8 (1 M)
353.3 μl of water (100 v/v)
E12
  • Sodium chloride 0.12 M -
  • MOPS 0.1 M pH 6
  • Polyethylene glycol 400 35 v/v -
pH 6
100.0 μl of MOPS pH 6 (1 M)
700.0 μl of Polyethylene glycol 400 (50 v/v)
60.0 μl of Sodium chloride (2 M)
140.0 μl of water (100 v/v)
F1
  • Ammonium formate 0.175 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 35 v/v -
pH 6.5
70.0 μl of Ammonium formate (2.5 M)
100.0 μl of MES pH 6.5 (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
480.0 μl of water (100 v/v)
F2
  • Sodium chloride 0.2 M -
  • MES 0.1 M pH 6.8
  • Polyethylene glycol 400 35 v/v -
pH 6.8
31.5 μl of MES pH 6.5 (1 M)
68.5 μl of MES pH 7 (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium chloride (2 M)
450.0 μl of water (100 v/v)
F3
  • Sodium chloride 0.05 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 200 35 v/v -
  • Magnesium acetate 0.05 M -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
50.0 μl of Magnesium acetate (1 M)
350.0 μl of Polyethylene glycol 200 (100 v/v)
50.0 μl of Sodium chloride (1 M)
450.0 μl of water (100 v/v)
F4
  • tri-Sodium citrate 0.1 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 1,000 32 w/v -
pH 6
100.0 μl of MES pH 6 (1 M)
640.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tri-Sodium citrate (1 M)
160.0 μl of water (100 v/v)
F5
  • Potassium nitrate 0.22 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol 400 30 v/v -
pH 6
300.0 μl of Polyethylene glycol 400 (100 v/v)
220.0 μl of Potassium nitrate (1 M)
77.5 μl of tri-Sodium citrate pH 6.2 (1 M)
22.5 μl of tri-Sodium citrate pH 5.8 (1 M)
380.0 μl of water (100 v/v)
F6
  • di-Sodium malonate 0.525 M pH 5.6
  • Polyethylene glycol 6,000 3 w/v -
pH 5.6
60.0 μl of Polyethylene glycol 6,000 (50 w/v)
94.2 μl of di-Sodium malonate pH 6 (3.4 M)
60.2 μl of di-Sodium malonate pH 5 (3.4 M)
785.6 μl of water (100 v/v)
F7
  • Lithium sulfate 0.16 M -
  • Strontium chloride 4 mM -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 40 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
160.0 μl of Lithium sulfate (1 M)
400.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Strontium chloride (200 mM)
320.0 μl of water (100 v/v)
F8
  • Sodium thiocyanate 0.1 M -
  • Calcium chloride 0.02 M -
  • MES 0.05 M pH 6.5
  • Polyethylene glycol 400 30 v/v -
pH 6.5
200.0 μl of Calcium chloride (0.1 M)
50.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of Sodium thiocyanate (2 M)
400.0 μl of water (100 v/v)
F9
  • Magnesium sulfate 0.025 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 300 42 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
25.0 μl of Magnesium sulfate (1 M)
420.0 μl of Polyethylene glycol 300 (100 v/v)
455.0 μl of water (100 v/v)
F10
  • Magnesium sulfate 0.075 M -
  • BICINE 0.1 M pH 8
  • Polyethylene glycol 400 30 v/v -
pH 8
100.0 μl of BICINE pH 8 (1 M)
75.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
525.0 μl of water (100 v/v)
F11
  • Magnesium sulfate 0.05 M -
  • Sodium chloride 0.15 M -
  • tri-Sodium citrate 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 30 v/v -
pH 6
50.0 μl of Magnesium sulfate (1 M)
300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
150.0 μl of Sodium chloride (1 M)
22.5 μl of tri-Sodium citrate pH 5.8 (1 M)
77.5 μl of tri-Sodium citrate pH 6.2 (1 M)
400.0 μl of water (100 v/v)
F12
  • Ammonium formate 0.025 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol 400 25 v/v -
pH 6
10.0 μl of Ammonium formate (2.5 M)
100.0 μl of MES pH 6 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
640.0 μl of water (100 v/v)
G1
  • di-Ammonium hydrogen citrate 0.1 M -
  • HEPES 0.1 M pH 8
  • Polyethylene glycol monomethyl ether 350 20 v/v -
pH 8
100.0 μl of HEPES pH 8 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
100.0 μl of di-Ammonium hydrogen citrate (1 M)
600.0 μl of water (100 v/v)
G2
  • Cadmium chloride 0.002 M -
  • MES 0.1 M pH 6
  • Polyethylene glycol dimethyl ether 500 28 v/v -
pH 6
20.0 μl of Cadmium chloride (0.1 M)
100.0 μl of MES pH 6 (1 M)
280.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v)
600.0 μl of water (100 v/v)
G3
  • Potassium Sodium tartrate 0.175 M -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Polyethylene glycol 400 32 v/v -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
175.0 μl of Potassium Sodium tartrate (1 M)
405.0 μl of water (100 v/v)
G4
  • di-Ammonium hydrogen phosphate 0.08 M -
  • HEPES 0.1 M pH 7.5
  • Polyethylene glycol 300 32 v/v -
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
320.0 μl of Polyethylene glycol 300 (100 v/v)
22.9 μl of di-Ammonium hydrogen phosphate (3.5 M)
557.1 μl of water (100 v/v)
G5
  • Sodium chloride 0.45 M -
  • MES 0.1 M pH 6.5
  • Polyethylene glycol 400 39 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
390.0 μl of Polyethylene glycol 400 (100 v/v)
225.0 μl of Sodium chloride (2 M)
285.0 μl of water (100 v/v)
G6
  • di-Ammonium hydrogen phosphate 0.12 M -
  • Sodium acetate 0.1 M pH 5
  • Polyethylene glycol 400 28 v/v -
pH 5
280.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
34.3 μl of di-Ammonium hydrogen phosphate (3.5 M)
585.7 μl of water (100 v/v)
G7
  • Sodium thiocyanate 0.05 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 25 v/v -
pH 5
250.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Sodium thiocyanate (2 M)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
625.0 μl of water (100 v/v)
G8
  • Magnesium acetate 0.1 M -
  • ADA 0.1 M pH 6.5
  • Polyethylene glycol 400 39 v/v -
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
100.0 μl of Magnesium acetate (1 M)
390.0 μl of Polyethylene glycol 400 (100 v/v)
410.0 μl of water (100 v/v)
G9
  • Ethylenediaminetetraacetic acid disodium salt 0.04 M -
  • HEPES 0.1 M pH 7
  • Polyethylene glycol 300 15 v/v -
pH 7
400.0 μl of Ethylenediaminetetraacetic acid disodium salt (0.1 M)
100.0 μl of HEPES pH 7 (1 M)
150.0 μl of Polyethylene glycol 300 (100 v/v)
350.0 μl of water (100 v/v)
G10
  • Sodium acetate 0.075 M pH 4.5
  • Sodium chloride 1.5 M -
pH 4.5
75.0 μl of Sodium acetate pH 4.5 (1 M)
300.0 μl of Sodium chloride (5 M)
625.0 μl of water (100 v/v)
G11
  • Magnesium formate 0.12 M -
  • Sodium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 8.5
  • Polyethylene glycol 600 33 v/v -
pH 8.5
120.0 μl of Magnesium formate (1 M)
330.0 μl of Polyethylene glycol 600 (100 v/v)
100.0 μl of Sodium chloride (1 M)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
350.0 μl of water (100 v/v)
G12
  • MES 0.1 M pH 6.5
  • Polyethylene glycol monomethyl ether 550 30 v/v -
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
600.0 μl of water (100 v/v)
H1
  • di-Sodium malonate 0.2 M -
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 29 v/v -
pH 8
580.0 μl of Polyethylene glycol 400 (50 v/v)
66.7 μl of di-Sodium malonate (3 M)
100.0 μl of tris chloride BCC pH 8 (1 M)
253.3 μl of water (100 v/v)
H2
  • Sodium sulfate 0.15 M -
  • BIS-TRIS 0.1 M pH 6.3
  • Polyethylene glycol 400 35 v/v -
pH 6.3
46.2 μl of BIS-TRIS pH 6.6 (1 M)
53.8 μl of BIS-TRIS pH 6 (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
150.0 μl of Sodium sulfate (1 M)
400.0 μl of water (100 v/v)
H3
  • Sodium acetate 0.1 M -
  • BIS-TRIS propane 0.1 M pH 8.3
  • Polyethylene glycol 300 25 v/v -
pH 8.3
84.8 μl of BIS-TRIS propane pH 9 (1 M)
15.2 μl of BIS-TRIS propane pH 7.5 (1 M)
250.0 μl of Polyethylene glycol 300 (100 v/v)
50.0 μl of Sodium acetate (2 M)
600.0 μl of water (100 v/v)
H4
  • Magnesium chloride 0.175 M -
  • tri-Sodium citrate 0.1 M pH 5
  • Polyethylene glycol 400 30 v/v -
pH 5
175.0 μl of Magnesium chloride (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tri-Sodium citrate pH 5 (1 M)
425.0 μl of water (100 v/v)
H5
  • Ammonium sulfate 0.55 M -
  • ADA 0.05 M pH 7
  • Polyethylene glycol monomethyl ether 550 26 v/v -
pH 7
50.0 μl of ADA pH 7 (1 M)
183.3 μl of Ammonium sulfate (3 M)
260.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
506.7 μl of water (100 v/v)
H6
  • Ammonium formate 0.03 M -
  • HEPES 0.05 M pH 7.5
  • Polyethylene glycol 200 38 v/v -
pH 7.5
12.0 μl of Ammonium formate (2.5 M)
50.0 μl of HEPES pH 7.5 (1 M)
380.0 μl of Polyethylene glycol 200 (100 v/v)
558.0 μl of water (100 v/v)
H7
  • Ammonium sulfate 0.1 M -
  • tri-Sodium citrate 0.2 M -
  • HEPES 0.1 M pH 8
  • Polyethylene glycol 200 35 v/v -
pH 8
50.0 μl of Ammonium sulfate (2 M)
100.0 μl of HEPES pH 8 (1 M)
350.0 μl of Polyethylene glycol 200 (100 v/v)
200.0 μl of tri-Sodium citrate (1 M)
300.0 μl of water (100 v/v)
H8
  • Potassium Sodium tartrate 0.15 M -
  • BIS-TRIS propane 0.1 M pH 6.5
  • Polyethylene glycol 400 25 v/v -
pH 6.5
100.0 μl of BIS-TRIS propane pH 6.5 (1 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium Sodium tartrate (1.5 M)
550.0 μl of water (100 v/v)
H9
  • Potassium formate 0.25 M -
  • Zinc sulfate 0.01 M -
  • Sodium acetate 0.1 M pH 4
  • Polyethylene glycol monomethyl ether 550 28 v/v -
pH 4
280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
125.0 μl of Potassium formate (2 M)
100.0 μl of Sodium acetate pH 4 (1 M)
10.0 μl of Zinc sulfate (1 M)
485.0 μl of water (100 v/v)
H10
  • tris chloride BCC 0.1 M pH 8
  • Polyethylene glycol 400 44 v/v -
pH 8
440.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
460.0 μl of water (100 v/v)
H11
  • tri-Lithium citrate 0.05 M -
  • HEPES 0.08 M pH 7
  • Polyethylene glycol 400 20 v/v -
pH 7
80.0 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
33.3 μl of tri-Lithium citrate (1.5 M)
686.7 μl of water (100 v/v)
H12
  • Sodium Potassium phosphate 3 M pH 5.5
pH 5.5
814.7 μl of Sodium Potassium phosphate pH 5 (3 M)
185.3 μl of Sodium Potassium phosphate pH 6.2 (3 M)