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This website is a work-in-progress and is actively being developed. As such the user experience may have room for improvement, or content may be incomplete.
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Supplemental Information
Screen Description
Molecular Dimensions / MemGoldMeso Eco
Created 2020-05-11.
Rigaku Recipe XML
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Barcodes
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Screen Structure
Well Count | 96 |
---|---|
Number of distinct ingredients | 67 |
Internal diversity (score mean dist) | 0.917 |
Screen Composition
ingredient type | MemGoldMeso Eco |
---|---|
Buffer | 126 |
Organic (non-volatile) | 54 |
Organic (volatile) | 1 |
Polymer | 77 |
Salt | 130 |
Well Contents
Buffers are shown in italics.
More information about buffers.
Primary factors are shown in bold.
More information about primary factors.
Well | Ingredient, Concentration, Units, pH | pH | Recipe 1000μl |
---|---|---|---|
A1 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 420.0 μl of Polyethylene glycol 400 (100 v/v) 87.5 μl of Sodium acetate (2 M) 392.5 μl of water (100 v/v) |
A2 |
| pH 5.5 | 133.3 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 12.5 μl of Lithium nitrate (8 M) 50.0 μl of Sodium chloride (2 M) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 704.2 μl of water (100 v/v) |
A3 |
| pH 6.8 | 100.0 μl of Lithium sulfate (2 M) 31.5 μl of MES pH 6.5 (1 M) 68.5 μl of MES pH 7 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 550.0 μl of water (100 v/v) |
A4 |
| pH 6 | 37.5 μl of di-Sodium malonate (2 M) 100.0 μl of MES pH 6 (1 M) 400.0 μl of Polyethylene glycol 300 (100 v/v) 462.5 μl of water (100 v/v) |
A5 |
| pH 7.2 | 80.0 μl of Lithium chloride (5 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 77.1 μl of tris chloride BCC pH 7 (1 M) 22.9 μl of tris chloride BCC pH 7.6 (1 M) 520.0 μl of water (100 v/v) |
A6 |
| pH 9 | 100.0 μl of BICINE pH 9 (1 M) 426.7 μl of Polyethylene glycol 600 (75 v/v) 37.5 μl of Sodium chloride (2 M) 435.8 μl of water (100 v/v) |
A7 |
| pH 6.3 | 83.3 μl of di-Sodium succinate (1.2 M) 37.5 μl of MES pH 6 (1 M) 62.5 μl of MES pH 6.5 (1 M) 320.0 μl of Polyethylene glycol 300 (100 v/v) 496.7 μl of water (100 v/v) |
A8 |
| pH 6.3 | 166.7 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 60.0 μl of Magnesium acetate (1 M) 50.0 μl of Sodium chloride (2 M) 43.1 μl of tri-Sodium citrate pH 6.5 (1 M) 6.9 μl of tri-Sodium citrate pH 5.8 (1 M) 673.3 μl of water (100 v/v) |
A9 |
| pH 5.5 | 100.0 μl of Lithium sulfate (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v) |
A10 |
| pH 6 | 50.0 μl of Magnesium sulfate (2 M) 50.0 μl of MOPS pH 6 (1 M) 310.0 μl of Polyethylene glycol monomethyl ether 500 (100 v/v) 100.0 μl of Sodium sulfate (1 M) 490.0 μl of water (100 v/v) |
A11 |
| pH 5 | 30.0 μl of Sodium chloride (5 M) 900.0 μl of Sodium Potassium phosphate pH 5 (1 M) 70.0 μl of water (100 v/v) |
A12 |
| pH 7.5 | 100.0 μl of HEPES pH 7.5 (1 M) 175.0 μl of Magnesium chloride (2 M) 330.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of tri-Sodium citrate (3 M) 345.0 μl of water (100 v/v) |
B1 |
| pH 5 | 300.0 μl of D-(+)-Trehalose (1 M) 333.3 μl of Sodium Potassium phosphate pH 5 (3 M) 366.7 μl of water (100 v/v) |
B2 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 110.0 μl of Lithium sulfate (2 M) 280.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v) 510.0 μl of water (100 v/v) |
B3 |
| pH 8 | 7.0 μl of Ammonium fluoride (10 M) 100.0 μl of HEPES pH 8 (1 M) 320.0 μl of Polyethylene glycol 400 (100 v/v) 573.0 μl of water (100 v/v) |
B4 |
| pH 4.2 | 100.0 μl of Lithium sulfate (2 M) 200.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of Sodium phosphate citrate pH 4.2 (1 M) 600.0 μl of water (100 v/v) |
B5 |
| pH 4.5 | 85.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of Magnesium chloride (0.1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 4.5 (1 M) 414.3 μl of water (100 v/v) |
B6 |
| pH 7.6 | 300.0 μl of Ammonium acetate (1 M) 20.5 μl of BIS-TRIS pH 7 (1 M) 79.5 μl of BIS-TRIS pH 8 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 300.0 μl of water (100 v/v) |
B7 |
| pH 3.6 | 340.0 μl of Polyethylene glycol 400 (100 v/v) 66.8 μl of tri-Sodium citrate pH 3.2 (1 M) 33.2 μl of tri-Sodium citrate pH 4 (1 M) 560.0 μl of water (100 v/v) |
B8 |
| pH 7.5 | 280.0 μl of Ammonium sulfate (2 M) 80.0 μl of HEPES pH 7.5 (1 M) 360.0 μl of Jeffamine® M-600 (50 v/v) 280.0 μl of water (100 v/v) |
B9 |
| pH 8 | 270.0 μl of Polyethylene glycol 400 (100 v/v) 40.0 μl of Sodium formate (5 M) 100.0 μl of tris chloride BCC pH 8 (1 M) 590.0 μl of water (100 v/v) |
B10 |
| pH 5.5 | 100.0 μl of BIS-TRIS pH 5.5 (1 M) 500.0 μl of Polyethylene glycol 3,350 (50 w/v) 400.0 μl of water (100 v/v) |
B11 |
| pH 5 | 220.0 μl of penta-Ethylene glycol (100 v/v) 50.0 μl of Sodium acetate pH 5 (1 M) 730.0 μl of water (100 v/v) |
B12 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 340.0 μl of Polyethylene glycol 400 (50 v/v) 120.0 μl of Potassium Sodium tartrate (1.5 M) 440.0 μl of water (100 v/v) |
C1 |
| pH 6 | 300.0 μl of Polyethylene glycol 400 (100 v/v) 225.0 μl of Potassium nitrate (2 M) 22.5 μl of tri-Sodium citrate pH 5.8 (1 M) 77.5 μl of tri-Sodium citrate pH 6.2 (1 M) 375.0 μl of water (100 v/v) |
C2 |
| pH 8.5 | 20.0 μl of Magnesium chloride (2 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 580.0 μl of water (100 v/v) |
C3 |
| pH 6 | 10.0 μl of Ammonium fluoride (10 M) 100.0 μl of MES pH 6 (1 M) 300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v) 33.3 μl of Sodium acetate (3 M) 556.7 μl of water (100 v/v) |
C4 |
| pH 7.5 | 28.6 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 450.0 μl of Polyethylene glycol 300 (100 v/v) 421.4 μl of water (100 v/v) |
C5 |
| pH 5.1 | 316.9 μl of Sodium Potassium phosphate pH 5 (3 M) 49.8 μl of Sodium Potassium phosphate pH 6.2 (3 M) 633.3 μl of water (100 v/v) |
C6 |
| pH 7.5 | 50.0 μl of Ammonium sulfate (3 M) 100.0 μl of HEPES pH 7.5 (1 M) 100.0 μl of Polyethylene glycol 400 (100 v/v) 750.0 μl of water (100 v/v) |
C7 |
| pH 8.5 | 240.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 50.0 μl of Sodium chloride (2 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 610.0 μl of water (100 v/v) |
C8 |
| pH 8 | 85.7 μl of di-Ammonium hydrogen phosphate (3.5 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 534.3 μl of water (100 v/v) |
C9 |
| pH 5.6 | 533.3 μl of Polyethylene glycol 2,000 (60 w/v) 81.9 μl of Sodium Potassium phosphate pH 5 (1 M) 18.1 μl of Sodium Potassium phosphate pH 6 (1 M) 366.7 μl of water (100 v/v) |
C10 |
| pH 5 | 116.7 μl of Ammonium acetate (3 M) 350.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 433.3 μl of water (100 v/v) |
C11 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 150.0 μl of Lithium sulfate (2 M) 350.0 μl of Polyethylene glycol 400 (100 v/v) 400.0 μl of water (100 v/v) |
C12 |
| pH 6.5 | 20.0 μl of Ammonium chloride (5 M) 10.0 μl of Calcium chloride (1 M) 100.0 μl of MES pH 6.5 (1 M) 200.0 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v) 670.0 μl of water (100 v/v) |
D1 |
| pH 6 | 71.4 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of MES pH 6 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 548.6 μl of water (100 v/v) |
D2 |
| pH 8.3 | 360.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of tri-Sodium citrate (1.6 M) 62.5 μl of TRICINE pH 8.5 (1 M) 37.5 μl of TRICINE pH 8 (1 M) 490.0 μl of water (100 v/v) |
D3 |
| pH 5.5 | 333.3 μl of 2-methyl-2,4-pentanediol BCC (15 v/v) 100.0 μl of Magnesium chloride (1 M) 50.0 μl of tri-Sodium citrate pH 5.5 (1 M) 516.7 μl of water (100 v/v) |
D4 |
| pH 7 | 100.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 80.0 μl of Sodium chloride (5 M) 620.0 μl of water (100 v/v) |
D5 |
| pH 7 | 100.0 μl of BIS-TRIS pH 7 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 21.4 μl of Potassium formate (14 M) 598.6 μl of water (100 v/v) |
D6 |
| pH 6 | 310.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Sodium formate (4 M) 22.5 μl of tri-Sodium citrate pH 5.8 (1 M) 77.5 μl of tri-Sodium citrate pH 6.5 (1 M) 540.0 μl of water (100 v/v) |
D7 |
| pH 8.3 | 175.0 μl of Magnesium sulfate (2 M) 280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 61.7 μl of tris chloride BCC pH 8 (1 M) 38.3 μl of tris chloride BCC pH 9 (1 M) 445.0 μl of water (100 v/v) |
D8 |
| pH 8.8 | 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of Potassium nitrate (1 M) 66.7 μl of Potassium Sodium tartrate (1.5 M) 12.2 μl of tris chloride BCC pH 8 (1 M) 87.8 μl of tris chloride BCC pH 9 (1 M) 433.3 μl of water (100 v/v) |
D9 |
| pH 9 | 500.0 μl of Ammonium sulfate (4 M) 100.0 μl of BICINE pH 9 (1 M) 400.0 μl of water (100 v/v) |
D10 |
| pH 4.5 | 50.0 μl of Lithium sulfate (2 M) 180.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of Sodium chloride (3 M) 100.0 μl of tri-Sodium citrate pH 4.5 (1 M) 636.7 μl of water (100 v/v) |
D11 |
| pH 5.5 | 266.7 μl of 2-methyl-2,4-pentanediol BCC (30 v/v) 100.0 μl of Potassium nitrate (1 M) 33.3 μl of Sodium chloride (3 M) 100.0 μl of tri-Sodium citrate pH 5.5 (1 M) 500.0 μl of water (100 v/v) |
D12 |
| pH 5.8 | 25.1 μl of ADA pH 5.5 (1 M) 24.9 μl of ADA pH 6 (1 M) 400.0 μl of Potassium Sodium tartrate (1.5 M) 550.0 μl of water (100 v/v) |
E1 |
| pH 8.5 | 450.0 μl of 1,4-Butanediol (40 v/v) 140.0 μl of Ammonium formate (2.5 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 310.0 μl of water (100 v/v) |
E2 |
| pH 6 | 150.0 μl of Ethylenediaminetetraacetic acid disodium salt (0.5 M) 100.0 μl of MES pH 6 (1 M) 250.0 μl of Polyethylene glycol 300 (100 v/v) 500.0 μl of water (100 v/v) |
E3 |
| pH 6.8 | 57.5 μl of HEPES pH 6.6 (1 M) 42.5 μl of HEPES pH 7 (1 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 20.0 μl of tri-Ammonium citrate (2.5 M) 600.0 μl of water (100 v/v) |
E4 |
| pH 4.5 | 100.0 μl of di-Sodium malonate (2 M) 240.0 μl of Polyethylene glycol 3,350 (50 w/v) 100.0 μl of Sodium acetate pH 4.5 (1 M) 560.0 μl of water (100 v/v) |
E5 |
| pH 5 | 270.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 25.0 μl of Zinc sulfate (1 M) 605.0 μl of water (100 v/v) |
E6 |
| pH 7.5 | 280.0 μl of Polyethylene glycol 400 (50 v/v) 100.0 μl of tris chloride BCC pH 7.5 (1 M) 620.0 μl of water (100 v/v) |
E7 |
| pH 6.5 | 50.0 μl of Ammonium nitrate (2 M) 100.0 μl of MES pH 6.5 (1 M) 270.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v) 580.0 μl of water (100 v/v) |
E8 |
| pH 6.8 | 57.1 μl of di-Ammonium hydrogen phosphate (3.5 M) 57.5 μl of HEPES pH 6.6 (1 M) 42.5 μl of HEPES pH 7 (1 M) 240.0 μl of Polyethylene glycol 400 (100 v/v) 602.9 μl of water (100 v/v) |
E9 |
| pH 7 | 100.0 μl of ADA pH 7 (1 M) 400.0 μl of Ammonium di-hydrogen phosphate (1 M) 340.0 μl of Polyethylene glycol 400 (100 v/v) 160.0 μl of water (100 v/v) |
E10 |
| pH 4 | 50.0 μl of Magnesium Citrate (1 M) 420.0 μl of Polyethylene glycol 200 (100 v/v) 100.0 μl of Sodium acetate pH 4 (1 M) 430.0 μl of water (100 v/v) |
E11 |
| pH 6 | 280.0 μl of Polyethylene glycol 400 (100 v/v) 266.7 μl of tri-Potassium citrate (1.5 M) 77.5 μl of tri-Sodium citrate pH 6.2 (1 M) 22.5 μl of tri-Sodium citrate pH 5.8 (1 M) 353.3 μl of water (100 v/v) |
E12 |
| pH 6 | 100.0 μl of MOPS pH 6 (1 M) 700.0 μl of Polyethylene glycol 400 (50 v/v) 60.0 μl of Sodium chloride (2 M) 140.0 μl of water (100 v/v) |
F1 |
| pH 6.5 | 70.0 μl of Ammonium formate (2.5 M) 100.0 μl of MES pH 6.5 (1 M) 350.0 μl of Polyethylene glycol 400 (100 v/v) 480.0 μl of water (100 v/v) |
F2 |
| pH 6.8 | 31.5 μl of MES pH 6.5 (1 M) 68.5 μl of MES pH 7 (1 M) 350.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium chloride (2 M) 450.0 μl of water (100 v/v) |
F3 |
| pH 6.5 | 50.0 μl of Magnesium acetate (1 M) 100.0 μl of MES pH 6.5 (1 M) 350.0 μl of Polyethylene glycol 200 (100 v/v) 50.0 μl of Sodium chloride (1 M) 450.0 μl of water (100 v/v) |
F4 |
| pH 6 | 100.0 μl of MES pH 6 (1 M) 640.0 μl of Polyethylene glycol 1,000 (50 w/v) 100.0 μl of tri-Sodium citrate (1 M) 160.0 μl of water (100 v/v) |
F5 |
| pH 6 | 300.0 μl of Polyethylene glycol 400 (100 v/v) 220.0 μl of Potassium nitrate (1 M) 77.5 μl of tri-Sodium citrate pH 6.2 (1 M) 22.5 μl of tri-Sodium citrate pH 5.8 (1 M) 380.0 μl of water (100 v/v) |
F6 |
| pH 5.6 | 94.2 μl of di-Sodium malonate pH 6 (3.4 M) 60.2 μl of di-Sodium malonate pH 5 (3.4 M) 60.0 μl of Polyethylene glycol 6,000 (50 w/v) 785.6 μl of water (100 v/v) |
F7 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 160.0 μl of Lithium sulfate (1 M) 400.0 μl of Polyethylene glycol 400 (100 v/v) 20.0 μl of Strontium chloride (200 mM) 320.0 μl of water (100 v/v) |
F8 |
| pH 6.5 | 200.0 μl of Calcium chloride (0.1 M) 50.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 50.0 μl of Sodium thiocyanate (2 M) 400.0 μl of water (100 v/v) |
F9 |
| pH 6.5 | 25.0 μl of Magnesium sulfate (1 M) 100.0 μl of MES pH 6.5 (1 M) 420.0 μl of Polyethylene glycol 300 (100 v/v) 455.0 μl of water (100 v/v) |
F10 |
| pH 8 | 100.0 μl of BICINE pH 8 (1 M) 75.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 525.0 μl of water (100 v/v) |
F11 |
| pH 6 | 50.0 μl of Magnesium sulfate (1 M) 300.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v) 150.0 μl of Sodium chloride (1 M) 22.5 μl of tri-Sodium citrate pH 5.8 (1 M) 77.5 μl of tri-Sodium citrate pH 6.2 (1 M) 400.0 μl of water (100 v/v) |
F12 |
| pH 6 | 10.0 μl of Ammonium formate (2.5 M) 100.0 μl of MES pH 6 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 640.0 μl of water (100 v/v) |
G1 |
| pH 8 | 100.0 μl of di-Ammonium hydrogen citrate (1 M) 100.0 μl of HEPES pH 8 (1 M) 200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v) 600.0 μl of water (100 v/v) |
G2 |
| pH 6 | 20.0 μl of Cadmium chloride (0.1 M) 100.0 μl of MES pH 6 (1 M) 280.0 μl of Polyethylene glycol dimethyl ether 500 (100 v/v) 600.0 μl of water (100 v/v) |
G3 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 320.0 μl of Polyethylene glycol 400 (100 v/v) 175.0 μl of Potassium Sodium tartrate (1 M) 405.0 μl of water (100 v/v) |
G4 |
| pH 7.5 | 22.9 μl of di-Ammonium hydrogen phosphate (3.5 M) 100.0 μl of HEPES pH 7.5 (1 M) 320.0 μl of Polyethylene glycol 300 (100 v/v) 557.1 μl of water (100 v/v) |
G5 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 390.0 μl of Polyethylene glycol 400 (100 v/v) 225.0 μl of Sodium chloride (2 M) 285.0 μl of water (100 v/v) |
G6 |
| pH 5 | 34.3 μl of di-Ammonium hydrogen phosphate (3.5 M) 280.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Sodium acetate pH 5 (1 M) 585.7 μl of water (100 v/v) |
G7 |
| pH 5 | 250.0 μl of Polyethylene glycol 400 (100 v/v) 25.0 μl of Sodium thiocyanate (2 M) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 625.0 μl of water (100 v/v) |
G8 |
| pH 6.5 | 100.0 μl of ADA pH 6.5 (1 M) 100.0 μl of Magnesium acetate (1 M) 390.0 μl of Polyethylene glycol 400 (100 v/v) 410.0 μl of water (100 v/v) |
G9 |
| pH 7 | 400.0 μl of Ethylenediaminetetraacetic acid disodium salt (0.1 M) 100.0 μl of HEPES pH 7 (1 M) 150.0 μl of Polyethylene glycol 300 (100 v/v) 350.0 μl of water (100 v/v) |
G10 |
| pH 4.5 | 75.0 μl of Sodium acetate pH 4.5 (1 M) 300.0 μl of Sodium chloride (5 M) 625.0 μl of water (100 v/v) |
G11 |
| pH 8.5 | 120.0 μl of Magnesium formate (1 M) 330.0 μl of Polyethylene glycol 600 (100 v/v) 100.0 μl of Sodium chloride (1 M) 100.0 μl of tris chloride BCC pH 8.5 (1 M) 350.0 μl of water (100 v/v) |
G12 |
| pH 6.5 | 100.0 μl of MES pH 6.5 (1 M) 300.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 600.0 μl of water (100 v/v) |
H1 |
| pH 8 | 66.7 μl of di-Sodium malonate (3 M) 580.0 μl of Polyethylene glycol 400 (50 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 253.3 μl of water (100 v/v) |
H2 |
| pH 6.3 | 46.2 μl of BIS-TRIS pH 6.6 (1 M) 53.8 μl of BIS-TRIS pH 6 (1 M) 350.0 μl of Polyethylene glycol 400 (100 v/v) 150.0 μl of Sodium sulfate (1 M) 400.0 μl of water (100 v/v) |
H3 |
| pH 8.3 | 84.8 μl of BIS-TRIS propane pH 9 (1 M) 15.2 μl of BIS-TRIS propane pH 7.5 (1 M) 250.0 μl of Polyethylene glycol 300 (100 v/v) 50.0 μl of Sodium acetate (2 M) 600.0 μl of water (100 v/v) |
H4 |
| pH 5 | 175.0 μl of Magnesium chloride (1 M) 300.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tri-Sodium citrate pH 5 (1 M) 425.0 μl of water (100 v/v) |
H5 |
| pH 7 | 50.0 μl of ADA pH 7 (1 M) 183.3 μl of Ammonium sulfate (3 M) 260.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 506.7 μl of water (100 v/v) |
H6 |
| pH 7.5 | 12.0 μl of Ammonium formate (2.5 M) 50.0 μl of HEPES pH 7.5 (1 M) 380.0 μl of Polyethylene glycol 200 (100 v/v) 558.0 μl of water (100 v/v) |
H7 |
| pH 8 | 50.0 μl of Ammonium sulfate (2 M) 100.0 μl of HEPES pH 8 (1 M) 350.0 μl of Polyethylene glycol 200 (100 v/v) 200.0 μl of tri-Sodium citrate (1 M) 300.0 μl of water (100 v/v) |
H8 |
| pH 6.5 | 100.0 μl of BIS-TRIS propane pH 6.5 (1 M) 250.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of Potassium Sodium tartrate (1.5 M) 550.0 μl of water (100 v/v) |
H9 |
| pH 4 | 280.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v) 125.0 μl of Potassium formate (2 M) 100.0 μl of Sodium acetate pH 4 (1 M) 10.0 μl of Zinc sulfate (1 M) 485.0 μl of water (100 v/v) |
H10 |
| pH 8 | 440.0 μl of Polyethylene glycol 400 (100 v/v) 100.0 μl of tris chloride BCC pH 8 (1 M) 460.0 μl of water (100 v/v) |
H11 |
| pH 7 | 80.0 μl of HEPES pH 7 (1 M) 200.0 μl of Polyethylene glycol 400 (100 v/v) 33.3 μl of tri-Lithium citrate (1.5 M) 686.7 μl of water (100 v/v) |
H12 |
| pH 5.5 | 828.2 μl of Sodium Potassium phosphate pH 5 (3 M) 171.8 μl of Sodium Potassium phosphate pH 6.2 (3 M) |