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Supplemental Information

Screen Description

MemTrans Eco

Molecular Dimensions / MemTrans Eco
Created 2020-05-11.

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MemTrans Eco

    Screen Structure

    MemTrans Eco

    Well Count 96
    Number of distinct ingredients 63
    Internal diversity (score mean dist) 0.920

    Screen Composition

    2025-06-14T13:47:24.430197 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemTrans Eco
    Buffer 119
    Organic (non-volatile) 42
    Polymer 98
    Salt 138

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemTrans Eco Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Ammonium thiocyanate 0.4 M -
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 4.3
    • Polyethylene glycol 4,000 10 w/v -
    pH 4.3
    20 μl of Sodium chloride (5 M)
    400 μl of Ammonium thiocyanate (1 M)
    100 μl of tri-Sodium citrate pH 4.3 (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    280 μl of water (100 v/v)
    A2
    • Lithium sulfate 0.1 M -
    • Polyethylene glycol 400 26 v/v -
    • ADA 0.1 M pH 5.2
    pH 5.2
    7 μl of ADA pH 6 (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    92 μl of ADA pH 5 (1 M)
    50 μl of Lithium sulfate (2 M)
    590 μl of water (100 v/v)
    A3
    • Potassium nitrate 0.14 M -
    • MES 0.1 M pH 7.1
    • Polyethylene glycol 3,350 14 w/v -
    pH 7.1
    70 μl of Potassium nitrate (2 M)
    81 μl of MES pH 7.4 (1 M)
    280 μl of Polyethylene glycol 3,350 (50 w/v)
    18 μl of MES pH 6.5 (1 M)
    550 μl of water (100 v/v)
    A4
    • Sodium chloride 0.125 M -
    • Calcium chloride 0.04 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 400 22 v/v -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    440 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of Calcium chloride (1 M)
    125 μl of Sodium chloride (1 M)
    295 μl of water (100 v/v)
    A5
    • Sodium chloride 0.05 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 200 30 v/v -
    pH 6.5
    50 μl of MES pH 6.5 (1 M)
    300 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Sodium chloride (1 M)
    600 μl of water (100 v/v)
    A6
    • Glycine 0.1 M pH 9
    • Polyethylene glycol monomethyl ether 350 38 v/v -
    pH 9
    380 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    100 μl of Glycine pH 9 (1 M)
    520 μl of water (100 v/v)
    A7
    • Calcium chloride 0.06 M -
    • Magnesium chloride 0.06 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    250 μl of Polyethylene glycol 4,000 (100 w/v)
    60 μl of Magnesium chloride (1 M)
    60 μl of Calcium chloride (1 M)
    100 μl of BICINE pH 8.5 (1 M)
    530 μl of water (100 v/v)
    A8
    • Ammonium sulfate 0.2 M -
    • Sodium chloride 0.02 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 400 24 v/v -
    pH 4
    200 μl of Ammonium sulfate (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (1 M)
    100 μl of Sodium acetate pH 4 (1 M)
    440 μl of water (100 v/v)
    A9
    • Potassium thiocyanate 0.2 M -
    • Glycine 0.1 M pH 9
    • Polyethylene glycol 3,350 8 w/v -
    pH 9
    160 μl of Polyethylene glycol 3,350 (50 w/v)
    25 μl of Potassium thiocyanate (8 M)
    100 μl of Glycine pH 9 (1 M)
    715 μl of water (100 v/v)
    A10
    • Sodium sulfate 0.15 M -
    • tris chloride BCC 0.025 M pH 7
    • Polyethylene glycol 400 29 v/v -
    pH 7
    290 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of tris chloride BCC pH 7 (1 M)
    150 μl of Sodium sulfate (1 M)
    535 μl of water (100 v/v)
    A11
    • Sodium chloride 0.2 M -
    • MES 0.05 M pH 6.2
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 6.2
    57 μl of MES pH 6 (0.5 M)
    200 μl of Sodium chloride (1 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    42 μl of MES pH 6.5 (0.5 M)
    360 μl of water (100 v/v)
    A12
    • Calcium chloride 0.1 M -
    • MES 0.05 M pH 5.5
    • Polyethylene glycol 400 29 v/v -
    pH 5.5
    50 μl of MES pH 5.5 (1 M)
    580 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Calcium chloride (1 M)
    270 μl of water (100 v/v)
    B1
    • Sodium chloride 0.115 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 18 v/v -
    pH 7
    360 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    115 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    425 μl of water (100 v/v)
    B2
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 2,000 10 w/v -
    pH 5.5
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    700 μl of water (100 v/v)
    B3
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 600 36 v/v -
    pH 7
    100 μl of MOPS pH 7 (1 M)
    360 μl of Polyethylene glycol 600 (100 v/v)
    540 μl of water (100 v/v)
    B4
    • tri-Potassium citrate 0.075 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 21 w/v -
    pH 5.5
    50 μl of tri-Potassium citrate (1.5 M)
    100 μl of MES pH 5.5 (1 M)
    420 μl of Polyethylene glycol monomethyl ether 550 (50 w/v)
    430 μl of water (100 v/v)
    B5
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 5
    • Polyethylene glycol 400 24 v/v -
    pH 5
    200 μl of Ammonium sulfate (1 M)
    480 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of MES pH 5 (1 M)
    220 μl of water (100 v/v)
    B6
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 4,000 12 w/v -
    pH 5
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium chloride (1 M)
    100 μl of Magnesium chloride (1 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    460 μl of water (100 v/v)
    B7
    • Magnesium chloride 0.225 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 2,000 20 w/v -
    pH 8.5
    112 μl of Magnesium chloride (2 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    400 μl of Polyethylene glycol 2,000 (50 w/v)
    387 μl of water (100 v/v)
    B8
    • Magnesium chloride 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 6.5
    100 μl of Magnesium chloride (2 M)
    50 μl of MES pH 6.5 (1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    690 μl of water (100 v/v)
    B9
    • Ammonium formate 0.1 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol 400 25 v/v -
    pH 6.2
    40 μl of Ammonium formate (2.5 M)
    100 μl of MES pH 6.2 (1 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    360 μl of water (100 v/v)
    B10
    • tris chloride BCC 0.05 M pH 8.7
    • Polyethylene glycol 400 28 v/v -
    pH 8.7
    24 μl of tris chloride BCC pH 9 (1 M)
    25 μl of tris chloride BCC pH 8.5 (1 M)
    560 μl of Polyethylene glycol 400 (50 v/v)
    390 μl of water (100 v/v)
    B11
    • Sodium chloride 0.1 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 400 25 v/v -
    pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    50 μl of Sodium chloride (2 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    400 μl of water (100 v/v)
    B12
    • Calcium chloride 0.055 M -
    • Potassium chloride 0.04 M -
    • MOPS 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 5 w/v -
    pH 7
    55 μl of Calcium chloride (1 M)
    100 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of MOPS pH 7 (1 M)
    40 μl of Potassium chloride (1 M)
    755 μl of water (100 v/v)
    C1
    • Sodium acetate 0.03 M -
    • Sodium chloride 0.02 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 24 v/v -
    pH 8
    15 μl of Sodium acetate (2 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    625 μl of water (100 v/v)
    C2
    • Magnesium chloride 0.25 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 2,000 23 w/v -
    pH 6.5
    125 μl of Magnesium chloride (2 M)
    460 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of MES pH 6.5 (0.5 M)
    315 μl of water (100 v/v)
    C3
    • Sodium chloride 0.06 M -
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 400 24 v/v -
    pH 4.5
    240 μl of Polyethylene glycol 400 (100 v/v)
    60 μl of Sodium chloride (1 M)
    50 μl of tri-Sodium citrate pH 4.5 (1 M)
    650 μl of water (100 v/v)
    C4
    • Magnesium nitrate 0.1 M -
    • tris chloride BCC 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 7
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Magnesium nitrate (1 M)
    550 μl of water (100 v/v)
    C5
    • Manganese (II) chloride 0.01 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    100 μl of Manganese (II) chloride (0.1 M)
    500 μl of water (100 v/v)
    C6
    • tri-Lithium citrate 0.05 M -
    • MOPS 0.05 M pH 6.5
    • Polyethylene glycol 1,000 30 w/v -
    pH 6.5
    600 μl of Polyethylene glycol 1,000 (50 w/v)
    33 μl of tri-Lithium citrate (1.5 M)
    50 μl of MOPS pH 6.5 (1 M)
    316 μl of water (100 v/v)
    C7
    • Lithium Phosphate 0.05 M -
    • Lithium sulfate 0.05 M -
    • Sodium acetate 0.025 M pH 5
    • Polyethylene glycol 300 32 v/v -
    pH 5
    50 μl of Lithium Phosphate (1 M)
    25 μl of Sodium acetate pH 5 (1 M)
    50 μl of Lithium sulfate (1 M)
    320 μl of Polyethylene glycol 300 (100 v/v)
    555 μl of water (100 v/v)
    C8
    • tri-Sodium citrate 0.05 M -
    • tri-Potassium citrate 0.05 M -
    • ADA 0.05 M pH 6.8
    • Polyethylene glycol 400 25 v/v -
    pH 6.8
    50 μl of ADA pH 6.8 (1 M)
    33 μl of tri-Potassium citrate (1.5 M)
    50 μl of tri-Sodium citrate (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    616 μl of water (100 v/v)
    C9
    • Nickel sulfate 0.05 M -
    • Lithium sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 30 v/v -
    pH 8.5
    300 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Lithium sulfate (1 M)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    12 μl of Nickel sulfate (4 M)
    587 μl of water (100 v/v)
    C10
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 9.5
    100 μl of CHES pH 9.5 (1 M)
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    200 μl of Sodium chloride (1 M)
    500 μl of water (100 v/v)
    C11
    • Lithium nitrate 0.05 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 550 30 v/v -
    pH 6.5
    600 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    100 μl of ADA pH 6.5 (1 M)
    6 μl of Lithium nitrate (8 M)
    293 μl of water (100 v/v)
    C12
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 6.2
    100 μl of Magnesium chloride (2 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of MES pH 6.2 (1 M)
    460 μl of water (100 v/v)
    D1
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.03 M pH 8
    • Polyethylene glycol 4,000 17 w/v -
    pH 8
    100 μl of Magnesium chloride (1 M)
    30 μl of tris chloride BCC pH 8 (1 M)
    340 μl of Polyethylene glycol 4,000 (50 w/v)
    530 μl of water (100 v/v)
    D2
    • Calcium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 300 28 v/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Calcium chloride (2 M)
    570 μl of water (100 v/v)
    D3
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.5
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    600 μl of water (100 v/v)
    D4
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6.2
    • Polyethylene glycol 300 30 v/v -
    pH 6.2
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Magnesium chloride (1 M)
    100 μl of MES pH 6.2 (1 M)
    100 μl of Sodium chloride (1 M)
    400 μl of water (100 v/v)
    D5
    • Polyethylene glycol 400 17 v/v -
    • tri-Potassium citrate 0.1 M pH 5.4
    pH 5.4
    340 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of tri-Potassium citrate pH 5.4 (1 M)
    560 μl of water (100 v/v)
    D6
    • Lithium sulfate 0.11 M -
    • Sodium Phosphate Monobasic 0.04 M -
    • tri-Sodium citrate 0.05 M pH 4.3
    • Polyethylene glycol 1,000 15 w/v -
    pH 4.3
    110 μl of Lithium sulfate (1 M)
    40 μl of Sodium Phosphate Monobasic (1 M)
    50 μl of tri-Sodium citrate pH 4.3 (1 M)
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    500 μl of water (100 v/v)
    D7
    • Sodium chloride 0.1 M -
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 36 v/v -
    pH 6.5
    360 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of MES pH 6.5 (1 M)
    50 μl of Calcium chloride (1 M)
    100 μl of Sodium chloride (1 M)
    390 μl of water (100 v/v)
    D8
    • Zinc chloride 0.12 M -
    • tri-Potassium citrate 0.1 M pH 4.2
    • Polyethylene glycol 300 40 v/v -
    pH 4.2
    400 μl of Polyethylene glycol 300 (100 v/v)
    120 μl of Zinc chloride (1 M)
    0 μl of tri-Potassium citrate pH 5.4 (1 M)
    100 μl of tri-Potassium citrate pH 4 (1 M)
    380 μl of water (100 v/v)
    D9
    • Lithium nitrate 0.1 M -
    • Glycine 0.1 M pH 9.5
    • Polyethylene glycol 400 45 v/v -
    pH 9.5
    12 μl of Lithium nitrate (8 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Glycine pH 9.5 (1 M)
    437 μl of water (100 v/v)
    D10
    • tri-Sodium citrate 0.06 M -
    • Sodium chloride 0.05 M -
    • HEPES 0.05 M pH 8
    • Polyethylene glycol monomethyl ether 550 28 v/v -
    pH 8
    50 μl of HEPES pH 8 (1 M)
    50 μl of Sodium chloride (1 M)
    280 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    37 μl of tri-Sodium citrate (1.6 M)
    582 μl of water (100 v/v)
    D11
    • Calcium acetate 0.1 M -
    • Lithium sulfate 0.02 M -
    • tris chloride BCC 0.1 M pH 7.2
    • Polyethylene glycol 400 32 v/v -
    pH 7.2
    320 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Lithium sulfate (1 M)
    100 μl of Calcium acetate (1 M)
    30 μl of tris chloride BCC pH 7.5 (1 M)
    69 μl of tris chloride BCC pH 7 (1 M)
    460 μl of water (100 v/v)
    D12
    • Praseodymium (III) acetate 0.01 M -
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 5.8
    • Polyethylene glycol 400 25 v/v -
    pH 5.8
    26 μl of Sodium acetate pH 5.6 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of Magnesium acetate (1 M)
    100 μl of Praseodymium (III) acetate (0.1 M)
    23 μl of Sodium acetate pH 6.2 (1 M)
    550 μl of water (100 v/v)
    E1
    • Ammonium acetate 0.2 M -
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6.3
    • Polyethylene glycol 4,000 12 w/v -
    pH 6.3
    43 μl of BIS-TRIS pH 6 (1 M)
    200 μl of Ammonium acetate (1 M)
    100 μl of Sodium chloride (1 M)
    56 μl of BIS-TRIS pH 6.5 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    360 μl of water (100 v/v)
    E2
    • Potassium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 27 v/v -
    • Polyethylene glycol 400 8 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    540 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    200 μl of Potassium chloride (1 M)
    80 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of water (100 v/v)
    E3
    • Calcium acetate 0.1 M -
    • Sodium chloride 0.1 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 24 v/v -
    pH 7
    480 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Sodium chloride (1 M)
    100 μl of MOPS pH 7 (1 M)
    100 μl of Calcium acetate (1 M)
    220 μl of water (100 v/v)
    E4
    • Magnesium chloride 0.2 M -
    • BICINE 0.1 M pH 8.5
    • Polyethylene glycol 2,000 21 w/v -
    pH 8.5
    200 μl of Magnesium chloride (1 M)
    420 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of BICINE pH 8.5 (1 M)
    280 μl of water (100 v/v)
    E5
    • Lithium sulfate 0.075 M -
    • Lithium Phosphate 0.075 M -
    • MOPS 0.1 M pH 7.4
    • Polyethylene glycol monomethyl ether 550 24 v/v -
    pH 7.4
    75 μl of Lithium Phosphate (1 M)
    19 μl of MOPS pH 7 (1 M)
    75 μl of Lithium sulfate (1 M)
    480 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    81 μl of MOPS pH 7.5 (1 M)
    270 μl of water (100 v/v)
    E6
    • Calcium chloride 0.045 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 8,000 5 w/v -
    pH 7
    125 μl of Polyethylene glycol 8,000 (40 w/v)
    100 μl of HEPES pH 7 (1 M)
    45 μl of Calcium chloride (1 M)
    730 μl of water (100 v/v)
    E7
    • Barium chloride 0.15 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 26 v/v -
    pH 7
    150 μl of Barium chloride (1 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    490 μl of water (100 v/v)
    E8
    • Sodium chloride 0.125 M -
    • Lithium sulfate 0.125 M -
    • HEPES 0.1 M pH 8
    • Polyethylene glycol 300 33 v/v -
    pH 8
    100 μl of HEPES pH 8 (1 M)
    125 μl of Lithium sulfate (1 M)
    330 μl of Polyethylene glycol 300 (100 v/v)
    125 μl of Sodium chloride (1 M)
    320 μl of water (100 v/v)
    E9
    • Magnesium nitrate 0.1 M -
    • Magnesium acetate 0.05 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 1,500 18 w/v -
    pH 5
    50 μl of Magnesium acetate (1 M)
    100 μl of tri-Sodium citrate pH 5 (1 M)
    360 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of Magnesium nitrate (1 M)
    390 μl of water (100 v/v)
    E10
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.08 M pH 9
    • Polyethylene glycol 600 17 v/v -
    pH 9
    100 μl of Sodium chloride (1 M)
    226 μl of Polyethylene glycol 600 (75 v/v)
    80 μl of tris chloride BCC pH 9 (1 M)
    593 μl of water (100 v/v)
    E11
    • Nickel (II) chloride 0.01 M -
    • MES 0.05 M pH 5.5
    • Polyethylene glycol 400 45 v/v -
    pH 5.5
    50 μl of MES pH 5.5 (1 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Nickel (II) chloride (1 M)
    490 μl of water (100 v/v)
    E12
    • Sodium chloride 0.4 M -
    • tri-Sodium citrate 0.05 M pH 6
    • Polyethylene glycol 400 37 v/v -
    pH 6
    370 μl of Polyethylene glycol 400 (100 v/v)
    80 μl of Sodium chloride (5 M)
    48 μl of tri-Sodium citrate pH 6.2 (1 M)
    1 μl of tri-Sodium citrate pH 5.9 (1 M)
    500 μl of water (100 v/v)
    F1
    • Lithium sulfate 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 400 20 v/v -
    pH 6.5
    200 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of MES pH 6.5 (1 M)
    100 μl of Lithium sulfate (2 M)
    650 μl of water (100 v/v)
    F2
    • Sodium chloride 0.075 M -
    • CHES 0.05 M pH 9.5
    • Polyethylene glycol 300 32 v/v -
    pH 9.5
    50 μl of CHES pH 9.5 (1 M)
    320 μl of Polyethylene glycol 300 (100 v/v)
    75 μl of Sodium chloride (1 M)
    555 μl of water (100 v/v)
    F3
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 600 26 v/v -
    pH 8
    260 μl of Polyethylene glycol 600 (100 v/v)
    200 μl of Sodium chloride (2 M)
    50 μl of tris chloride BCC pH 8 (1 M)
    490 μl of water (100 v/v)
    F4
    • Polyethylene glycol 3,350 15 w/v -
    • di-Sodium succinate 0.1 M pH 7
    pH 7
    100 μl of di-Sodium succinate pH 7 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    F5
    • Calcium acetate 0.08 M -
    • Sodium chloride 0.05 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 28 v/v -
    pH 7
    50 μl of Sodium chloride (1 M)
    100 μl of MOPS pH 7 (1 M)
    80 μl of Calcium acetate (1 M)
    560 μl of Polyethylene glycol 400 (50 v/v)
    210 μl of water (100 v/v)
    F6
    • Sodium chloride 0.07 M -
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 400 22 v/v -
    • Polyethylene glycol 600 2 v/v -
    pH 4.5
    40 μl of Polyethylene glycol 600 (50 v/v)
    220 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of tri-Sodium citrate pH 4.5 (1 M)
    70 μl of Sodium chloride (1 M)
    620 μl of water (100 v/v)
    F7
    • di-Sodium malonate 0.2 M -
    • HEPES 0.075 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    66 μl of di-Sodium malonate (3 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    75 μl of HEPES pH 7 (1 M)
    458 μl of water (100 v/v)
    F8
    • Potassium di-hydrogen phosphate 0.08 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 400 35 v/v -
    pH 6.5
    80 μl of Potassium di-hydrogen phosphate (1 M)
    50 μl of ADA pH 6.5 (1 M)
    700 μl of Polyethylene glycol 400 (50 v/v)
    170 μl of water (100 v/v)
    F9
    • Lithium sulfate 0.1 M -
    • HEPES 0.05 M pH 7
    • Polyethylene glycol 3,350 31 w/v -
    pH 7
    100 μl of Lithium sulfate (1 M)
    620 μl of Polyethylene glycol 3,350 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    230 μl of water (100 v/v)
    F10
    • Potassium acetate 0.25 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 24 v/v -
    pH 8
    240 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Potassium acetate (5 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    610 μl of water (100 v/v)
    F11
    • Magnesium acetate 0.125 M -
    • Magnesium chloride 0.04 M -
    • tris chloride BCC 0.08 M pH 8.7
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.7
    41 μl of tris chloride BCC pH 8.5 (1 M)
    40 μl of Magnesium chloride (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    125 μl of Magnesium acetate (1 M)
    38 μl of tris chloride BCC pH 9 (1 M)
    515 μl of water (100 v/v)
    F12
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.7
    • Polyethylene glycol monomethyl ether 550 38 v/v -
    pH 8.7
    380 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    51 μl of tris chloride BCC pH 8.5 (1 M)
    100 μl of Magnesium chloride (1 M)
    48 μl of tris chloride BCC pH 9 (1 M)
    420 μl of water (100 v/v)
    G1
    • Nickel (II) chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 4
    100 μl of Sodium acetate pH 4 (1 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    50 μl of Nickel (II) chloride (1 M)
    510 μl of water (100 v/v)
    G2
    • Magnesium chloride 0.05 M -
    • MES 0.1 M pH 5.5
    • Polyethylene glycol 400 40 v/v -
    pH 5.5
    100 μl of MES pH 5.5 (1 M)
    50 μl of Magnesium chloride (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    G3
    • Sodium chloride 0.15 M -
    • MOPS 0.075 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    150 μl of Sodium chloride (1 M)
    75 μl of MOPS pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    375 μl of water (100 v/v)
    G4
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 300 30 v/v -
    • 2-methyl-2,4-pentanediol BCC 3 v/v -
    pH 4.5
    200 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
    300 μl of Polyethylene glycol 300 (100 v/v)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    G5
    • Ammonium sulfate 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 22 v/v -
    pH 7.5
    100 μl of Ammonium sulfate (1 M)
    50 μl of HEPES pH 7.5 (1 M)
    440 μl of Polyethylene glycol 400 (50 v/v)
    410 μl of water (100 v/v)
    G6
    • Ammonium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 25 v/v -
    pH 6.5
    66 μl of Ammonium sulfate (3 M)
    100 μl of MES pH 6.5 (1 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    333 μl of water (100 v/v)
    G7
    • Calcium chloride 0.12 M -
    • BIS-TRIS 0.075 M pH 6.3
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.3
    42 μl of BIS-TRIS pH 6.5 (1 M)
    120 μl of Calcium chloride (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    32 μl of BIS-TRIS pH 6 (1 M)
    505 μl of water (100 v/v)
    G8
    • Cadmium chloride 0.005 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 31 v/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    620 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of Cadmium chloride (0.1 M)
    230 μl of water (100 v/v)
    G9
    • Potassium chloride 0.1 M -
    • MES 0.1 M pH 5
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 5
    440 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    100 μl of Potassium chloride (1 M)
    100 μl of MES pH 5 (1 M)
    360 μl of water (100 v/v)
    G10
    • Lithium chloride 0.1 M -
    • Glycine 0.05 M pH 9.5
    • Polyethylene glycol 400 40 v/v -
    pH 9.5
    25 μl of Glycine pH 9.5 (2 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Lithium chloride (1 M)
    475 μl of water (100 v/v)
    G11
    • tri-Sodium citrate 0.05 M -
    • Potassium di-hydrogen phosphate 0.08 M -
    • BIS-TRIS 0.08 M pH 6.3
    • Polyethylene glycol 4,000 11 w/v -
    • Potassium chloride 0.06 M -
    pH 6.3
    45 μl of BIS-TRIS pH 6.5 (1 M)
    80 μl of Potassium di-hydrogen phosphate (1 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    34 μl of BIS-TRIS pH 6 (1 M)
    60 μl of Potassium chloride (1 M)
    31 μl of tri-Sodium citrate (1.6 M)
    528 μl of water (100 v/v)
    G12
    • Calcium chloride 0.05 M -
    • tris chloride BCC 0.02 M pH 7.4
    • Polyethylene glycol 3,000 14 w/v -
    pH 7.4
    14 μl of tris chloride BCC pH 7.5 (1 M)
    50 μl of Calcium chloride (1 M)
    280 μl of Polyethylene glycol 3,000 (50 w/v)
    5 μl of tris chloride BCC pH 7 (1 M)
    650 μl of water (100 v/v)
    H1
    • Zinc sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 4.5
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Zinc sulfate (1 M)
    100 μl of Sodium acetate pH 4.5 (1 M)
    400 μl of water (100 v/v)
    H2
    • Sodium chloride 0.3 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 21 v/v -
    pH 5.5
    420 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    300 μl of Sodium chloride (1 M)
    180 μl of water (100 v/v)
    H3
    • Magnesium chloride 0.08 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,500 19 w/v -
    pH 8.5
    100 μl of tris chloride BCC pH 8.5 (1 M)
    80 μl of Magnesium chloride (1 M)
    380 μl of Polyethylene glycol 1,500 (50 w/v)
    440 μl of water (100 v/v)
    H4
    • Sodium chloride 0.125 M -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 34 v/v -
    pH 8.5
    50 μl of Magnesium chloride (2 M)
    340 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    125 μl of Sodium chloride (1 M)
    385 μl of water (100 v/v)
    H5
    • Magnesium chloride 0.05 M -
    • Potassium chloride 0.02 M -
    • MES 0.05 M pH 5.8
    • Polyethylene glycol 200 40 v/v -
    pH 5.8
    400 μl of Polyethylene glycol 200 (100 v/v)
    50 μl of Magnesium chloride (1 M)
    20 μl of Potassium chloride (1 M)
    50 μl of MES pH 5.8 (1 M)
    480 μl of water (100 v/v)
    H6
    • Ammonium acetate 0.2 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 3,350 24 w/v -
    pH 8.5
    50 μl of tris chloride BCC pH 8.5 (1 M)
    200 μl of Ammonium acetate (1 M)
    480 μl of Polyethylene glycol 3,350 (50 w/v)
    270 μl of water (100 v/v)
    H7
    • Lithium sulfate 0.08 M -
    • Sodium Phosphate Monobasic 0.06 M -
    • tri-Sodium citrate 0.05 M pH 4.7
    • Polyethylene glycol 1,000 16 w/v -
    pH 4.7
    80 μl of Lithium sulfate (1 M)
    60 μl of Sodium Phosphate Monobasic (1 M)
    320 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of tri-Sodium citrate pH 4.7 (1 M)
    490 μl of water (100 v/v)
    H8
    • Potassium thiocyanate 125 mM -
    • Calcium chloride 0.01 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 4,000 8 w/v -
    pH 7.5
    250 μl of Potassium thiocyanate (500 mM)
    50 μl of HEPES pH 7.5 (1 M)
    100 μl of Calcium chloride (0.1 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    440 μl of water (100 v/v)
    H9
    • Magnesium nitrate 0.06 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 550 24 v/v -
    pH 5.5
    100 μl of tri-Sodium citrate pH 5.5 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    60 μl of Magnesium nitrate (1 M)
    600 μl of water (100 v/v)
    H10
    • Sodium chloride 80 mM -
    • Lithium sulfate 25 mM -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 27 v/v -
    pH 7.5
    270 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of HEPES pH 7.5 (1 M)
    160 μl of Sodium chloride (500 mM)
    250 μl of Lithium sulfate (100 mM)
    270 μl of water (100 v/v)
    H11
    • Sodium chloride 0.5 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 40 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    166 μl of Sodium chloride (3 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    333 μl of water (100 v/v)
    H12
    • Ammonium di-hydrogen phosphate 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 1,500 20 w/v -
    pH 7
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    100 μl of HEPES pH 7 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    300 μl of water (100 v/v)