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Supplemental Information

Screen Description

MemGold Eco

Molecular Dimensions / MemGold Eco
Created 2020-05-11.

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MemGold Eco

    Screen Structure

    MemGold Eco

    Well Count 96
    Number of distinct ingredients 59
    Internal diversity (score mean dist) 0.921

    Screen Composition

    2025-06-14T14:01:12.436050 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemGold Eco
    Buffer 114
    Cryoprotectant 1
    Organic (non-volatile) 31
    Polymer 89
    Salt 128

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemGold Eco Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • tri-Sodium citrate 0.08 M pH 5.2
    • Ammonium sulfate 2.2 M -
    pH 5.2
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    628 μl of Ammonium sulfate (3.5 M)
    46 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    321 μl of water (100 v/v)
    A2
    • tris chloride BCC 0.01 M pH 8
    • tri-Sodium citrate 1.2 M -
    pH 8
    4 μl of tris chloride BCC pH 9 (1 M)
    750 μl of tri-Sodium citrate (1.6 M)
    5 μl of tris chloride BCC pH 7 (1 M)
    240 μl of water (100 v/v)
    A3
    • TRICINE 0.015 M pH 8.5
    • Polyethylene glycol 4,000 24 w/v -
    pH 8.5
    480 μl of Polyethylene glycol 4,000 (50 w/v)
    2 μl of TRICINE pH 7.4 (1 M)
    12 μl of TRICINE pH 8.8 (1 M)
    505 μl of water (100 v/v)
    A4
    • Sodium chloride 0.36 M -
    • Sodium azide 0.1 w/v -
    • Sodium di-hydrogen phosphate 0.015 M pH 7
    • Polyethylene glycol 4,000 9.9 w/v -
    pH 7
    198 μl of Polyethylene glycol 4,000 (50 w/v)
    1 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    100 μl of Sodium azide (1 w/v)
    1 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    72 μl of Sodium chloride (5 M)
    627 μl of water (100 v/v)
    A5
    • Sodium chloride 0.3 M -
    • tris chloride BCC 0.01 M pH 8
    • Polyethylene glycol 4,000 27.5 w/v -
    pH 8
    4 μl of tris chloride BCC pH 9 (1 M)
    550 μl of Polyethylene glycol 4,000 (50 w/v)
    5 μl of tris chloride BCC pH 7 (1 M)
    60 μl of Sodium chloride (5 M)
    380 μl of water (100 v/v)
    A6
    • MES 0.225 M pH 6.6
    • Polyethylene glycol 6,000 6.6 w/v -
    • BIS-TRIS 0.225 M pH 6.6
    NA
    68 μl of BIS-TRIS pH 5.5 (1 M)
    84 μl of MES pH 5.2 (0.5 M)
    132 μl of Polyethylene glycol 6,000 (50 w/v)
    156 μl of BIS-TRIS pH 7 (1 M)
    365 μl of MES pH 7.1 (0.5 M)
    193 μl of water (100 v/v)
    A7
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 12 w/v -
    • Glycerol 22 v/v -
    pH 7.5
    28 μl of Ammonium sulfate (3.5 M)
    51 μl of HEPES pH 6.6 (1 M)
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    220 μl of Glycerol (100 v/v)
    48 μl of HEPES pH 8.5 (1 M)
    411 μl of water (100 v/v)
    A8
    • Calcium chloride 0.02 M -
    • Magnesium sulfate 0.01 M -
    • Sodium chloride 0.02 M -
    • MES 0.02 M pH 6.5
    • Polyethylene glycol 1,500 7.7 w/v -
    pH 6.5
    154 μl of Polyethylene glycol 1,500 (50 w/v)
    4 μl of Magnesium sulfate (2.5 M)
    9 μl of MES pH 5.2 (0.5 M)
    10 μl of Calcium chloride (2 M)
    30 μl of MES pH 7.1 (0.5 M)
    4 μl of Sodium chloride (5 M)
    788 μl of water (100 v/v)
    A9
    • HEPES 0.05 M pH 7.5
    • Ammonium sulfate 2.5 M -
    pH 7.5
    714 μl of Ammonium sulfate (3.5 M)
    25 μl of HEPES pH 6.6 (1 M)
    24 μl of HEPES pH 8.5 (1 M)
    235 μl of water (100 v/v)
    A10
    • HEPES 0.0665 M pH 7.5
    • tri-Sodium citrate 1.1 M -
    pH 7.5
    34 μl of HEPES pH 6.6 (1 M)
    687 μl of tri-Sodium citrate (1.6 M)
    32 μl of HEPES pH 8.5 (1 M)
    246 μl of water (100 v/v)
    A11
    • Potassium Sodium phosphate 0.15 M pH 6.5
    • Ammonium sulfate 3.3 M -
    pH 6.5
    942 μl of Ammonium sulfate (3.5 M)
    50 μl of Potassium Sodium phosphate pH 5.5 (3 M)
    0 μl of Potassium Sodium phosphate pH 7.5 (3 M)
    7 μl of water (100 v/v)
    A12
    • Magnesium acetate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.8
    • Polyethylene glycol monomethyl ether 5,000 14 w/v -
    pH 5.8
    2 μl of tri-Sodium citrate pH 3 (1.6 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    100 μl of Magnesium acetate (1 M)
    280 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
    557 μl of water (100 v/v)
    B1
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.02 M pH 5.6
    • Polyethylene glycol 3,350 11 w/v -
    pH 5.6
    0 μl of tri-Sodium citrate pH 3 (1.6 M)
    11 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    220 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Sodium chloride (5 M)
    747 μl of water (100 v/v)
    B2
    • Sodium chloride 0.1 M -
    • tri-Sodium citrate 0.02 M pH 5.6
    • Polyethylene glycol 3,350 5.5 w/v -
    pH 5.6
    0 μl of tri-Sodium citrate pH 3 (1.6 M)
    11 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    110 μl of Polyethylene glycol 3,350 (50 w/v)
    20 μl of Sodium chloride (5 M)
    857 μl of water (100 v/v)
    B3
    • Calcium chloride 0.05 M -
    • Barium chloride 0.05 M -
    • tris chloride BCC 0.1 M pH 8.2
    • Polyethylene glycol 400 32 v/v -
    pH 8.2
    59 μl of tris chloride BCC pH 9 (1 M)
    25 μl of Calcium chloride (2 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    40 μl of tris chloride BCC pH 7 (1 M)
    500 μl of Barium chloride (0.1 M)
    55 μl of water (100 v/v)
    B4
    • Sodium chloride 0.05 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 6.2
    • Polyethylene glycol 4,000 16 w/v -
    pH 6.2
    320 μl of Polyethylene glycol 4,000 (50 w/v)
    1 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    18 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    10 μl of Sodium chloride (5 M)
    650 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.03 M pH 8.2
    • Polyethylene glycol 4,000 19 w/v -
    pH 8.2
    50 μl of Magnesium chloride (2 M)
    380 μl of Polyethylene glycol 4,000 (50 w/v)
    17 μl of tris chloride BCC pH 8 (1 M)
    12 μl of tris chloride BCC pH 8.5 (1 M)
    540 μl of water (100 v/v)
    B6
    • Sodium chloride 0.2 M -
    • HEPES 0.025 M pH 7.5
    • Polyethylene glycol 4,000 13 w/v -
    pH 7.5
    12 μl of HEPES pH 6.6 (1 M)
    260 μl of Polyethylene glycol 4,000 (50 w/v)
    12 μl of HEPES pH 8.5 (1 M)
    40 μl of Sodium chloride (5 M)
    675 μl of water (100 v/v)
    B7
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 3,350 11 w/v -
    pH 7.5
    51 μl of HEPES pH 6.6 (1 M)
    220 μl of Polyethylene glycol 3,350 (50 w/v)
    48 μl of HEPES pH 8.5 (1 M)
    680 μl of water (100 v/v)
    B8
    • Sodium chloride 0.1 M -
    • Potassium MES 0.02 M pH 6.7
    • Polyethylene glycol 4,000 6.6 w/v -
    pH 6.7
    2 μl of Potassium MES pH 5.2 (1 M)
    132 μl of Polyethylene glycol 4,000 (50 w/v)
    17 μl of Potassium MES pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    828 μl of water (100 v/v)
    B9
    • Potassium chloride 0.1 M -
    • tris chloride BCC 0.02 M pH 7
    • Polyethylene glycol 4,000 20 w/v -
    pH 7
    0 μl of tris chloride BCC pH 9 (1 M)
    400 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of tris chloride BCC pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    555 μl of water (100 v/v)
    B10
    • Magnesium chloride 0.05 M -
    • Sodium azide 0.1 w/v -
    • MES 0.1 M pH 6.7
    • Polyethylene glycol 3,350 6.6 w/v -
    pH 6.7
    25 μl of Magnesium chloride (2 M)
    20 μl of MES pH 6 (1 M)
    100 μl of Sodium azide (1 w/v)
    132 μl of Polyethylene glycol 3,350 (50 w/v)
    80 μl of MES pH 7 (1 M)
    643 μl of water (100 v/v)
    B11
    • Potassium chloride 0.2 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Pentaerythritol Propoxylate (5/4 PO/OH) 37 v/v -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    740 μl of Pentaerythritol Propoxylate (5/4 PO/OH) (50 v/v)
    50 μl of Potassium chloride (4 M)
    147 μl of water (100 v/v)
    B12
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 4,000 5.5 w/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    110 μl of Polyethylene glycol 4,000 (50 w/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    790 μl of water (100 v/v)
    C1
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.02 M pH 7
    • Polyethylene glycol 4,000 7.7 w/v -
    pH 7
    0 μl of tris chloride BCC pH 9 (1 M)
    154 μl of Polyethylene glycol 4,000 (50 w/v)
    20 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    806 μl of water (100 v/v)
    C2
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 400 22 v/v -
    pH 7.5
    15 μl of tris chloride BCC pH 9 (1 M)
    50 μl of Magnesium chloride (2 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    84 μl of tris chloride BCC pH 7 (1 M)
    630 μl of water (100 v/v)
    C3
    • Sodium chloride 0.04 M -
    • tris chloride BCC 0.04 M pH 8
    • Polyethylene glycol monomethyl ether 350 27 v/v -
    pH 8
    18 μl of tris chloride BCC pH 9 (1 M)
    270 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    8 μl of Sodium chloride (5 M)
    682 μl of water (100 v/v)
    C4
    • Sodium chloride 0.05 M -
    • Magnesium chloride 0.02 M -
    • tri-Sodium citrate 0.1 M pH 6
    • Polyethylene glycol 400 22 v/v -
    pH 6
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    10 μl of Magnesium chloride (2 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Sodium chloride (5 M)
    697 μl of water (100 v/v)
    C5
    • Sodium acetate 0.1 M pH 5.5
    • Polyethylene glycol monomethyl ether 2,000 8.8 w/v -
    pH 5.5
    100 μl of Sodium acetate pH 5.5 (1 M)
    176 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    724 μl of water (100 v/v)
    C6
    • Ammonium acetate 0.4 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 13 w/v -
    pH 8
    0 μl of Ammonium acetate pH 4 (1 M)
    260 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    399 μl of Ammonium acetate pH 10 (1 M)
    340 μl of water (100 v/v)
    C7
    • BIS-TRIS 0.02 M pH 7
    • Polyethylene glycol 2,000 15 w/v -
    pH 7
    300 μl of Polyethylene glycol 2,000 (50 w/v)
    20 μl of BIS-TRIS pH 7 (1 M)
    680 μl of water (100 v/v)
    C8
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.02 M pH 7.5
    • Polyethylene glycol 1,500 11 w/v -
    pH 7.5
    3 μl of tris chloride BCC pH 9 (1 M)
    220 μl of Polyethylene glycol 1,500 (50 w/v)
    50 μl of Magnesium chloride (2 M)
    16 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    690 μl of water (100 v/v)
    C9
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 8
    • Polyethylene glycol 1,500 11 w/v -
    pH 8
    220 μl of Polyethylene glycol 1,500 (50 w/v)
    18 μl of HEPES pH 6.6 (1 M)
    50 μl of Magnesium chloride (2 M)
    81 μl of HEPES pH 8.5 (1 M)
    20 μl of Sodium chloride (5 M)
    610 μl of water (100 v/v)
    C10
    • Sodium acetate 0.2 M -
    • Potassium chloride 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 3,000 22 w/v -
    pH 7
    66 μl of Sodium acetate (3 M)
    83 μl of HEPES pH 6.6 (1 M)
    440 μl of Polyethylene glycol 3,000 (50 w/v)
    16 μl of HEPES pH 8.5 (1 M)
    50 μl of Potassium chloride (4 M)
    343 μl of water (100 v/v)
    C11
    • Nickel sulfate 0.02 M -
    • HEPES 0.01 M pH 7
    • Jeffamine® M-600 33 v/v -
    pH 7
    8 μl of HEPES pH 6.6 (1 M)
    5 μl of Nickel sulfate (4 M)
    660 μl of Jeffamine® M-600 pH 7 (50 v/v)
    1 μl of HEPES pH 8.5 (1 M)
    325 μl of water (100 v/v)
    C12
    • Sodium chloride 0.15 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 6,000 13 w/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    260 μl of Polyethylene glycol 6,000 (50 w/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    30 μl of Sodium chloride (5 M)
    610 μl of water (100 v/v)
    D1
    • Calcium chloride 0.2 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 53 v/v -
    pH 7.5
    51 μl of HEPES pH 6.6 (1 M)
    100 μl of Calcium chloride (2 M)
    48 μl of HEPES pH 8.5 (1 M)
    530 μl of Polyethylene glycol 400 (100 v/v)
    270 μl of water (100 v/v)
    D2
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 5
    • Polyethylene glycol 400 28 v/v -
    pH 5
    15 μl of Sodium acetate pH 4 (1 M)
    34 μl of Sodium acetate pH 5.5 (1 M)
    50 μl of Magnesium acetate (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    620 μl of water (100 v/v)
    D3
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 4,000 22 w/v -
    pH 7.5
    25 μl of HEPES pH 6.6 (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    24 μl of HEPES pH 8.5 (1 M)
    510 μl of water (100 v/v)
    D4
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 44 v/v -
    pH 8
    100 μl of Calcium chloride (2 M)
    440 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of tris chloride BCC pH 8 (1 M)
    360 μl of water (100 v/v)
    D5
    • Magnesium acetate 0.05 M -
    • Sodium acetate 0.05 M pH 5.4
    • Polyethylene glycol 400 24 v/v -
    pH 5.4
    42 μl of Sodium acetate pH 5.5 (1 M)
    7 μl of Sodium acetate pH 5 (1 M)
    50 μl of Magnesium acetate (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    660 μl of water (100 v/v)
    D6
    • Calcium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 350 26 v/v -
    pH 6.5
    260 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    100 μl of Calcium chloride (2 M)
    151 μl of MES pH 7.1 (0.5 M)
    440 μl of water (100 v/v)
    D7
    • Potassium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 39 v/v -
    pH 8.5
    78 μl of tris chloride BCC pH 9 (1 M)
    390 μl of Polyethylene glycol 400 (100 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    25 μl of Potassium chloride (4 M)
    485 μl of water (100 v/v)
    D8
    • Magnesium chloride 0.05 M -
    • Glycine 0.1 M pH 9
    • Polyethylene glycol 400 22 v/v -
    pH 9
    25 μl of Magnesium chloride (2 M)
    100 μl of Glycine pH 9 (1 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    655 μl of water (100 v/v)
    D9
    • Ammonium sulfate 0.1 M -
    • Glycine 0.1 M pH 3.8
    • Triethylene glycol 28 w/v -
    pH 3.8
    28 μl of Ammonium sulfate (3.5 M)
    280 μl of Triethylene glycol (100 w/v)
    50 μl of Glycine pH 3.8 (2 M)
    641 μl of water (100 v/v)
    D10
    • Sodium formate 0.15 M -
    • HEPES 0.1 M pH 7.2
    • Polyethylene glycol 3,350 18 w/v -
    pH 7.2
    72 μl of HEPES pH 6.6 (1 M)
    21 μl of Sodium formate (7 M)
    360 μl of Polyethylene glycol 3,350 (50 w/v)
    27 μl of HEPES pH 8.5 (1 M)
    518 μl of water (100 v/v)
    D11
    • Sodium acetate 0.2 M pH 6.8
    • Polyethylene glycol 6,000 8.8 w/v -
    pH 6.8
    200 μl of Sodium acetate pH 6.8 (1 M)
    176 μl of Polyethylene glycol 6,000 (50 w/v)
    624 μl of water (100 v/v)
    D12
    • Potassium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 6,000 18 w/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    360 μl of Polyethylene glycol 6,000 (50 w/v)
    151 μl of MES pH 7.1 (0.5 M)
    50 μl of Potassium chloride (4 M)
    390 μl of water (100 v/v)
    E1
    • tri-Sodium citrate 0.22 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 35 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    137 μl of tri-Sodium citrate (1.6 M)
    350 μl of Polyethylene glycol 400 (100 v/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    412 μl of water (100 v/v)
    E2
    • Sodium acetate 0.1 M pH 4.5
    • Polyethylene glycol 400 17 v/v -
    pH 4.5
    71 μl of Sodium acetate pH 4 (1 M)
    28 μl of Sodium acetate pH 5.5 (1 M)
    170 μl of Polyethylene glycol 400 (100 v/v)
    730 μl of water (100 v/v)
    E3
    • tris chloride BCC 0.02 M pH 8.5
    • Lithium sulfate 1 M -
    • Polyethylene glycol 8,000 1.8 w/v -
    pH 8.5
    36 μl of Polyethylene glycol 8,000 (50 w/v)
    500 μl of Lithium sulfate (2 M)
    20 μl of tris chloride BCC pH 8.5 (1 M)
    444 μl of water (100 v/v)
    E4
    • tris chloride BCC 0.02 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 7.5
    220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    20 μl of tris chloride BCC pH 7.5 (1 M)
    760 μl of water (100 v/v)
    E5
    • Sodium chloride 0.05 M -
    • Glycine 0.02 M pH 10
    • Polyethylene glycol 1,000 33 w/v -
    pH 10
    20 μl of Glycine pH 10 (1 M)
    660 μl of Polyethylene glycol 1,000 (50 w/v)
    10 μl of Sodium chloride (5 M)
    310 μl of water (100 v/v)
    E6
    • Magnesium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 4,000 25 w/v -
    pH 8.5
    100 μl of Magnesium chloride (2 M)
    500 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    300 μl of water (100 v/v)
    E7
    • Magnesium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 2,000 31 w/v -
    pH 6.5
    100 μl of Magnesium chloride (2 M)
    620 μl of Polyethylene glycol 2,000 (50 w/v)
    100 μl of MES pH 6.5 (1 M)
    180 μl of water (100 v/v)
    E8
    • Sodium acetate 0.64 M pH 4.6
    • Polyethylene glycol 3,350 18 w/v -
    pH 4.6
    410 μl of Sodium acetate pH 4 (1 M)
    229 μl of Sodium acetate pH 5.5 (1 M)
    360 μl of Polyethylene glycol 3,350 (50 w/v)
    E9
    • Sodium chloride 0.1 M -
    • Cadmium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 33 v/v -
    pH 8
    330 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Cadmium chloride (1 M)
    100 μl of tris chloride BCC pH 8 (1 M)
    20 μl of Sodium chloride (5 M)
    450 μl of water (100 v/v)
    E10
    • BICINE 0.1 M pH 8.9
    • Polyethylene glycol 2,000 31 w/v -
    pH 8.9
    86 μl of BICINE pH 9.3 (1 M)
    13 μl of BICINE pH 7.4 (1 M)
    620 μl of Polyethylene glycol 2,000 (50 w/v)
    280 μl of water (100 v/v)
    E11
    • Sodium sulfate 0.05 M -
    • Lithium sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 35 v/v -
    pH 8.5
    25 μl of Lithium sulfate (2 M)
    50 μl of Sodium sulfate (1 M)
    350 μl of Polyethylene glycol 400 (100 v/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    525 μl of water (100 v/v)
    E12
    • Sodium chloride 0.1 M -
    • Glycine 0.05 M pH 9.5
    • Polyethylene glycol 300 33 v/v -
    pH 9.5
    20 μl of Glycine pH 10 (1 M)
    330 μl of Polyethylene glycol 300 (100 v/v)
    29 μl of Glycine pH 9 (1 M)
    20 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    F1
    • Magnesium nitrate 0.3 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 2,000 23 w/v -
    pH 8
    100 μl of Magnesium nitrate (3 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    460 μl of Polyethylene glycol 2,000 (50 w/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    340 μl of water (100 v/v)
    F2
    • Lithium sulfate 0.12 M -
    • tris chloride BCC 0.02 M pH 7.5
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 300 20 v/v -
    NA
    5 μl of tri-Sodium citrate pH 3 (1.6 M)
    60 μl of Lithium sulfate (2 M)
    56 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    200 μl of Polyethylene glycol 300 (100 v/v)
    20 μl of tris chloride BCC pH 7.5 (1 M)
    657 μl of water (100 v/v)
    F3
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.12 M pH 9
    • Polyethylene glycol 400 20 v/v -
    pH 9
    120 μl of tris chloride BCC pH 9 (1 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    0 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    660 μl of water (100 v/v)
    F4
    • Sodium chloride 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 550 22 v/v -
    pH 7
    220 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of HEPES pH 7 (1 M)
    40 μl of Sodium chloride (5 M)
    640 μl of water (100 v/v)
    F5
    • Sodium chloride 0.1 M -
    • Sodium acetate 0.325 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 21 v/v -
    pH 8
    45 μl of tris chloride BCC pH 9 (1 M)
    108 μl of Sodium acetate (3 M)
    210 μl of Polyethylene glycol 400 (100 v/v)
    54 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    561 μl of water (100 v/v)
    F6
    • tri-Sodium citrate 0.02 M -
    • Sodium di-hydrogen phosphate 0.08 M pH 6.2
    • Polyethylene glycol 2,000 18 w/v -
    pH 6.2
    12 μl of tri-Sodium citrate (1.6 M)
    360 μl of Polyethylene glycol 2,000 (50 w/v)
    1 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    15 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    611 μl of water (100 v/v)
    F7
    • Potassium nitrate 0.02 M -
    • tri-Potassium citrate 0.03 M pH 6.5
    • Polyethylene glycol 4,000 7.7 w/v -
    pH 6.5
    6 μl of tri-Potassium citrate pH 6.5 (5 M)
    154 μl of Polyethylene glycol 4,000 (50 w/v)
    6 μl of Potassium nitrate (3 M)
    833 μl of water (100 v/v)
    F8
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.005 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 2,000 30 w/v -
    pH 8.5
    78 μl of tris chloride BCC pH 9 (1 M)
    2 μl of Magnesium chloride (2 M)
    600 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    20 μl of Sodium chloride (5 M)
    277 μl of water (100 v/v)
    F9
    • Cadmium chloride 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 33 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    200 μl of Cadmium chloride (1 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    370 μl of water (100 v/v)
    F10
    • Calcium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 13 w/v -
    pH 6.5
    100 μl of tris chloride BCC pH 6.5 (1 M)
    50 μl of Calcium chloride (2 M)
    260 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    590 μl of water (100 v/v)
    F11
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.02 M pH 4
    • Polyethylene glycol 200 33 v/v -
    • Sodium chloride 0.02 M -
    pH 4
    20 μl of Sodium acetate pH 4 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    330 μl of Polyethylene glycol 200 (100 v/v)
    4 μl of Sodium chloride (5 M)
    588 μl of water (100 v/v)
    F12
    • Sodium chloride 0.07 M -
    • tri-Sodium citrate 0.05 M pH 4.5
    • Polyethylene glycol 400 22 v/v -
    pH 4.5
    4 μl of tri-Sodium citrate pH 3 (1.6 M)
    27 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    220 μl of Polyethylene glycol 400 (100 v/v)
    14 μl of Sodium chloride (5 M)
    734 μl of water (100 v/v)
    G1
    • Ammonium sulfate 0.2 M -
    • Sodium acetate 0.1 M pH 4.6
    • Polyethylene glycol monomethyl ether 550 28 v/v -
    pH 4.6
    64 μl of Sodium acetate pH 4 (1 M)
    57 μl of Ammonium sulfate (3.5 M)
    35 μl of Sodium acetate pH 5.5 (1 M)
    280 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    562 μl of water (100 v/v)
    G2
    • Glycine 0.05 M pH 9
    • Polyethylene glycol 400 55 v/v -
    pH 9
    50 μl of Glycine pH 9 (1 M)
    550 μl of Polyethylene glycol 400 (100 v/v)
    400 μl of water (100 v/v)
    G3
    • Magnesium chloride 0.1 M -
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 400 33 v/v -
    pH 8.5
    50 μl of Magnesium chloride (2 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    100 μl of tris chloride BCC pH 8.5 (1 M)
    500 μl of water (100 v/v)
    G4
    • Lithium sulfate 0.1 M -
    • di-Sodium hydrogen phosphate 0.05 M -
    • tri-Sodium citrate 0.05 M pH 1
    • Polyethylene glycol 1,000 19 w/v -
    pH 1
    100 μl of di-Sodium hydrogen phosphate (0.5 M)
    50 μl of tri-Sodium citrate pH 1 (1 M)
    50 μl of Lithium sulfate (2 M)
    380 μl of Polyethylene glycol 1,000 (50 w/v)
    420 μl of water (100 v/v)
    G5
    • Magnesium chloride 0.2 M -
    • Potassium chloride 0.1 M -
    • tri-Sodium citrate 0.025 M pH 4
    • Polyethylene glycol 400 33 v/v -
    pH 4
    2 μl of tri-Sodium citrate pH 3 (1.6 M)
    100 μl of Magnesium chloride (2 M)
    13 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of Potassium chloride (4 M)
    529 μl of water (100 v/v)
    G6
    • Zinc acetate 0.05 M -
    • MES 0.05 M pH 6.1
    • Polyethylene glycol 8,000 11 w/v -
    pH 6.1
    220 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Zinc acetate (1 M)
    51 μl of MES pH 5.2 (0.5 M)
    48 μl of MES pH 7.1 (0.5 M)
    630 μl of water (100 v/v)
    G7
    • Magnesium nitrate 0.3 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 8,000 22 w/v -
    pH 8
    440 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Magnesium nitrate (3 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    54 μl of tris chloride BCC pH 7 (1 M)
    360 μl of water (100 v/v)
    G8
    • Sodium chloride 0.1 M -
    • Ethylene glycol 4 v/v -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 33 v/v -
    pH 6.5
    40 μl of Ethylene glycol (100 v/v)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Sodium chloride (5 M)
    410 μl of water (100 v/v)
    G9
    • Sodium chloride 0.05 M -
    • tri-Sodium citrate 0.1 M pH 5.5
    • Polyethylene glycol 400 26 v/v -
    pH 5.5
    3 μl of tri-Sodium citrate pH 3 (1.6 M)
    58 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    260 μl of Polyethylene glycol 400 (100 v/v)
    10 μl of Sodium chloride (5 M)
    667 μl of water (100 v/v)
    G10
    • Lithium sulfate 0.1 M -
    • Glycine 0.1 M pH 9.3
    • Polyethylene glycol 400 30 v/v -
    pH 9.3
    50 μl of Lithium sulfate (2 M)
    21 μl of Glycine pH 10 (1 M)
    78 μl of Glycine pH 9 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    550 μl of water (100 v/v)
    G11
    • tri-Potassium citrate 0.15 M -
    • tri-Lithium citrate 0.05 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 4.2
    • Polyethylene glycol 6,000 22 w/v -
    pH 4.2
    33 μl of tri-Lithium citrate (1.5 M)
    60 μl of tri-Potassium citrate (2.5 M)
    20 μl of Sodium di-hydrogen phosphate pH 4.2 (5 M)
    440 μl of Polyethylene glycol 6,000 (50 w/v)
    446 μl of water (100 v/v)
    G12
    • Zinc sulfate 0.001 M -
    • HEPES 0.05 M pH 7.8
    • Polyethylene glycol 600 28 v/v -
    pH 7.8
    0 μl of Zinc sulfate (2 M)
    280 μl of Polyethylene glycol 600 (100 v/v)
    50 μl of HEPES pH 7.8 (1 M)
    669 μl of water (100 v/v)
    H1
    • Sodium chloride 0.1 M -
    • Sodium di-hydrogen phosphate 0.1 M pH 7
    • Polyethylene glycol 300 33 v/v -
    pH 7
    330 μl of Polyethylene glycol 300 (100 v/v)
    8 μl of Sodium di-hydrogen phosphate pH 8 (5 M)
    11 μl of Sodium di-hydrogen phosphate pH 5.8 (5 M)
    20 μl of Sodium chloride (5 M)
    630 μl of water (100 v/v)
    H2
    • Sodium chloride 0.1 M -
    • BICINE 0.05 M pH 9
    • Polyethylene glycol 300 33 v/v -
    pH 9
    45 μl of BICINE pH 9.3 (1 M)
    330 μl of Polyethylene glycol 300 (100 v/v)
    4 μl of BICINE pH 7.4 (1 M)
    20 μl of Sodium chloride (5 M)
    600 μl of water (100 v/v)
    H3
    • Zinc acetate 0.05 M -
    • Ethylene glycol 6 v/v -
    • MES 0.1 M pH 6
    • Polyethylene glycol 8,000 6.6 w/v -
    pH 6
    132 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of Zinc acetate (1 M)
    60 μl of Ethylene glycol (100 v/v)
    100 μl of MES pH 6 (1 M)
    658 μl of water (100 v/v)
    H4
    • Lithium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 3.5
    • Polyethylene glycol 400 28 v/v -
    pH 3.5
    13 μl of tri-Sodium citrate pH 3 (1.6 M)
    100 μl of Lithium sulfate (2 M)
    49 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    557 μl of water (100 v/v)
    H5
    • Sodium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 7.5
    • Polyethylene glycol 4,000 11 w/v -
    pH 7.5
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tris chloride BCC pH 7.5 (1 M)
    20 μl of Sodium chloride (5 M)
    660 μl of water (100 v/v)
    H6
    • Lithium sulfate 0.05 M -
    • TRICINE 0.1 M pH 7.4
    • Polyethylene glycol 3,000 7 w/v -
    pH 7.4
    25 μl of Lithium sulfate (2 M)
    100 μl of TRICINE pH 7.4 (1 M)
    140 μl of Polyethylene glycol 3,000 (50 w/v)
    735 μl of water (100 v/v)
    H7
    • Calcium chloride 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 33 v/v -
    pH 6.5
    48 μl of MES pH 5.2 (0.5 M)
    100 μl of Calcium chloride (2 M)
    151 μl of MES pH 7.1 (0.5 M)
    330 μl of Polyethylene glycol 400 (100 v/v)
    370 μl of water (100 v/v)
    H8
    • Sodium chloride 1 M -
    • tri-Sodium citrate 0.1 M pH 6
    • Polyethylene glycol 4,000 28 w/v -
    pH 6
    1 μl of tri-Sodium citrate pH 3 (1.6 M)
    60 μl of tri-Sodium citrate pH 6.5 (1.6 M)
    560 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Sodium chloride (5 M)
    177 μl of water (100 v/v)
    H9
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 11 w/v -
    pH 7.5
    100 μl of HEPES pH 7.5 (1 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    680 μl of water (100 v/v)
    H10
    • Zinc sulfate 0.002 M -
    • HEPES 0.08 M pH 7
    • Jeffamine® ED-2001 25 v/v -
    pH 7
    1 μl of Zinc sulfate (2 M)
    80 μl of HEPES pH 7 (1 M)
    500 μl of Jeffamine® ED-2001 (50 v/v)
    419 μl of water (100 v/v)
    H11
    • Cadmium chloride 0.001 M -
    • Magnesium chloride 0.03 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 30 v/v -
    pH 6.5
    15 μl of Magnesium chloride (2 M)
    1 μl of Cadmium chloride (1 M)
    48 μl of MES pH 5.2 (0.5 M)
    151 μl of MES pH 7.1 (0.5 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    484 μl of water (100 v/v)
    H12
    • BIS-TRIS propane 0.1 M pH 7
    • Sodium chloride 3 M -
    pH 7
    100 μl of BIS-TRIS propane pH 7 (1 M)
    600 μl of Sodium chloride (5 M)
    300 μl of water (100 v/v)