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Supplemental Information

Screen Description

MemGold 2 Eco

Molecular Dimensions / MemGold 2 Eco
Created 2020-05-11.

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MemGold 2 Eco

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Screen Structure

MemGold 2 Eco

Well Count 96
Number of distinct ingredients 62
Internal diversity (score mean dist) 0.936

Screen Composition

2025-08-16T06:25:37.793026 image/svg+xml Matplotlib v3.10.5, https://matplotlib.org/
ingredient type MemGold 2 Eco
Buffer 120
Organic (non-volatile) 33
Polymer 85
Salt 112

Well Contents

Buffers are shown in italics. More information about buffers.
Primary factors are shown in bold. More information about primary factors.
MemGold 2 Eco Well Contents  🔍
WellIngredient, Concentration, Units, pHpHRecipe 1000μl
A1
  • Polyethylene glycol monomethyl ether 550 14 v/v -
  • Magnesium chloride 0.2 M -
  • Cadmium chloride 0.005 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
5.0 μl of Cadmium chloride (1 M)
100.0 μl of Magnesium chloride (2 M)
140.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
655.0 μl of water (100 v/v)
A2
  • Polyethylene glycol 3,000 44 w/v -
  • Potassium acetate 0.1 M -
  • Potassium chloride 0.01 M -
  • tris chloride BCC 0.02 M pH 7
pH 7
880.0 μl of Polyethylene glycol 3,000 (50 w/v)
20.0 μl of Potassium acetate (5 M)
2.5 μl of Potassium chloride (4 M)
20.0 μl of tris chloride BCC pH 7 (1 M)
77.5 μl of water (100 v/v)
A3
  • Polyethylene glycol 1,450 10 w/v -
  • Magnesium sulfate 0.08 M -
  • Sodium chloride 0.02 M -
  • MES 0.02 M pH 6
pH 6
23.4 μl of MES pH 5.2 (0.5 M)
16.6 μl of MES pH 7.1 (0.5 M)
32.0 μl of Magnesium sulfate (2.5 M)
200.0 μl of Polyethylene glycol 1,450 (50 w/v)
4.0 μl of Sodium chloride (5 M)
724.0 μl of water (100 v/v)
A4
  • Polyethylene glycol 1,450 8 w/v -
  • Calcium chloride 0.02 M -
  • Magnesium sulfate 0.04 M -
  • MES 0.02 M pH 6.5
pH 6.5
10.0 μl of Calcium chloride (2 M)
9.7 μl of MES pH 5.2 (0.5 M)
30.3 μl of MES pH 7.1 (0.5 M)
16.0 μl of Magnesium sulfate (2.5 M)
160.0 μl of Polyethylene glycol 1,450 (50 w/v)
774.0 μl of water (100 v/v)
A5
  • Polyethylene glycol 400 32 v/v -
  • Nickel sulfate 0.05 M -
  • Lithium chloride 0.05 M -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
5.0 μl of Lithium chloride (10 M)
12.5 μl of Nickel sulfate (4 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
612.5 μl of water (100 v/v)
A6
  • Polyethylene glycol 3,350 10 w/v -
  • di-Sodium hydrogen phosphate 0.1 M -
  • Potassium di-hydrogen phosphate 0.1 M -
  • BIS-TRIS propane 0.1 M pH 7.5
pH 7.5
100.0 μl of BIS-TRIS propane pH 7.5 (1 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of Potassium di-hydrogen phosphate (2 M)
25.0 μl of di-Sodium hydrogen phosphate (4 M)
625.0 μl of water (100 v/v)
A7
  • Polyethylene glycol 4,000 11.5 w/v -
  • Sodium chloride 0.1 M -
  • Lithium sulfate 0.1 M -
  • ADA 0.1 M pH 6.5
pH 6.5
100.0 μl of ADA pH 6.5 (1 M)
50.0 μl of Lithium sulfate (2 M)
230.0 μl of Polyethylene glycol 4,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
600.0 μl of water (100 v/v)
A8
  • Polyethylene glycol 400 30 v/v -
  • Cadmium chloride 0.1 M -
  • Lithium chloride 0.1 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
100.0 μl of Cadmium chloride (1 M)
10.0 μl of Lithium chloride (10 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
490.0 μl of water (100 v/v)
A9
  • Polyethylene glycol 2,000 20 w/v -
  • Ammonium sulfate 0.2 M -
  • Sodium chloride 0.1 M -
  • tri-Sodium citrate 0.1 M pH 6
pH 6
57.1 μl of Ammonium sulfate (3.5 M)
400.0 μl of Polyethylene glycol 2,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
14.1 μl of tri-Sodium citrate pH 3 (1.6 M)
48.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
460.4 μl of water (100 v/v)
A10
  • Polyethylene glycol 400 31 v/v -
  • Lithium sulfate 0.2 M -
  • Sodium chloride 0.1 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
100.0 μl of Lithium sulfate (2 M)
310.0 μl of Polyethylene glycol 400 (100 v/v)
20.0 μl of Sodium chloride (5 M)
470.0 μl of water (100 v/v)
A11
  • Polyethylene glycol 400 32 v/v -
  • Ammonium di-hydrogen phosphate 0.2 M -
  • Ammonium sulfate 0.1 M -
  • tri-Sodium citrate 0.1 M pH 4.5
pH 4.5
80.0 μl of Ammonium di-hydrogen phosphate (2.5 M)
28.6 μl of Ammonium sulfate (3.5 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
35.3 μl of tri-Sodium citrate pH 3 (1.6 M)
27.2 μl of tri-Sodium citrate pH 6.5 (1.6 M)
508.9 μl of water (100 v/v)
A12
  • Polyethylene glycol 4,000 14 w/v -
  • tri-Sodium citrate 0.05 M -
  • Potassium chloride 0.12 M -
  • BIS-TRIS 0.08 M pH 6
pH 6
62.1 μl of BIS-TRIS pH 5.5 (1 M)
17.9 μl of BIS-TRIS pH 7 (1 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
30.0 μl of Potassium chloride (4 M)
31.2 μl of tri-Sodium citrate (1.6 M)
578.8 μl of water (100 v/v)
B1
  • Polyethylene glycol 1,000 19 w/v -
  • Sodium chloride 0.1 M -
  • Ammonium sulfate 0.1 M -
  • MES 0.01 M pH 6.5
pH 6.5
28.6 μl of Ammonium sulfate (3.5 M)
4.9 μl of MES pH 5.2 (0.5 M)
15.1 μl of MES pH 7.1 (0.5 M)
380.0 μl of Polyethylene glycol 1,000 (50 w/v)
20.0 μl of Sodium chloride (5 M)
551.4 μl of water (100 v/v)
B2
  • Polyethylene glycol monomethyl ether 2,000 18 w/v -
  • Nickel sulfate 0.01 M -
  • tri-Sodium citrate 0.1 M pH 6
pH 6
2.5 μl of Nickel sulfate (4 M)
360.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
14.1 μl of tri-Sodium citrate pH 3 (1.6 M)
48.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
575.0 μl of water (100 v/v)
B3
  • Polyethylene glycol 3,350 3.5 w/v -
  • Magnesium chloride 0.02 M -
  • MES 0.02 M pH 6
pH 6
23.4 μl of MES pH 5.2 (0.5 M)
16.6 μl of MES pH 7.1 (0.5 M)
10.0 μl of Magnesium chloride (2 M)
70.0 μl of Polyethylene glycol 3,350 (50 w/v)
880.0 μl of water (100 v/v)
B4
  • Polyethylene glycol monomethyl ether 350 14 v/v -
  • Sodium chloride 0.02 M -
  • MES 0.05 M pH 5.5
pH 5.5
50.0 μl of MES pH 5.5 (1 M)
140.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
4.0 μl of Sodium chloride (5 M)
806.0 μl of water (100 v/v)
B5
  • Polyethylene glycol monomethyl ether 550 35 v/v -
  • Magnesium chloride 0.025 M -
  • MOPS 0.02 M pH 7
pH 7
13.4 μl of MOPS pH 6.5 (1 M)
6.6 μl of MOPS pH 7.9 (1 M)
12.5 μl of Magnesium chloride (2 M)
350.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
617.5 μl of water (100 v/v)
B6
  • Polyethylene glycol 400 28 v/v -
  • Magnesium chloride 0.03 M -
  • MES 0.1 M pH 6.5
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
15.0 μl of Magnesium chloride (2 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
505.0 μl of water (100 v/v)
B7
  • Polyethylene glycol monomethyl ether 350 25 v/v -
  • Sodium chloride 0.04 M -
  • tris chloride BCC 0.04 M pH 8
pH 8
250.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
8.0 μl of Sodium chloride (5 M)
40.0 μl of tris chloride BCC pH 8 (1 M)
702.0 μl of water (100 v/v)
B8
  • 2-methyl-2,4-pentanediol BCC 36 v/v -
  • Magnesium acetate 0.04 M -
  • MES 0.1 M pH 6
pH 6
360.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
40.0 μl of Magnesium acetate (1 M)
400.0 μl of water (100 v/v)
B9
  • Polyethylene glycol 8,000 11 w/v -
  • Zinc acetate 0.05 M -
  • ADA 0.05 M pH 6.3
pH 6.3
22.5 μl of ADA pH 6 (1 M)
27.5 μl of ADA pH 6.5 (1 M)
220.0 μl of Polyethylene glycol 8,000 (50 w/v)
50.0 μl of Zinc acetate (1 M)
680.0 μl of water (100 v/v)
B10
  • Polyethylene glycol 400 26 v/v -
  • Magnesium acetate 0.05 M -
  • MES 0.1 M pH 6.5
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
50.0 μl of Magnesium acetate (1 M)
260.0 μl of Polyethylene glycol 400 (100 v/v)
490.0 μl of water (100 v/v)
B11
  • Polyethylene glycol 400 32 v/v -
  • Magnesium acetate 0.05 M -
  • Glycine 0.1 M pH 9.5
pH 9.5
58.6 μl of Glycine pH 9 (1 M)
41.4 μl of Glycine pH 10 (1 M)
50.0 μl of Magnesium acetate (1 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
530.0 μl of water (100 v/v)
B12
  • Polyethylene glycol 4,000 3 w/v -
  • Sodium chloride 0.066 M -
  • tris chloride BCC 0.02 M pH 7.5
pH 7.5
60.0 μl of Polyethylene glycol 4,000 (50 w/v)
13.2 μl of Sodium chloride (5 M)
20.0 μl of tris chloride BCC pH 7.5 (1 M)
906.8 μl of water (100 v/v)
C1
  • Polyethylene glycol monomethyl ether 2,000 30 w/v -
  • Magnesium chloride 0.075 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
37.5 μl of Magnesium chloride (2 M)
600.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
262.5 μl of water (100 v/v)
C2
  • Polyethylene glycol monomethyl ether 5,000 14 w/v -
  • Magnesium acetate 0.08 M -
  • tri-Sodium citrate 0.1 M pH 6
pH 6
80.0 μl of Magnesium acetate (1 M)
280.0 μl of Polyethylene glycol monomethyl ether 5,000 (50 w/v)
14.1 μl of tri-Sodium citrate pH 3 (1.6 M)
48.4 μl of tri-Sodium citrate pH 6.5 (1.6 M)
577.5 μl of water (100 v/v)
C3
  • Ammonium sulfate 2 M -
  • Zinc acetate 0.05 M -
  • MES 0.1 M pH 6
pH 6
571.4 μl of Ammonium sulfate (3.5 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
50.0 μl of Zinc acetate (1 M)
178.6 μl of water (100 v/v)
C4
  • Polyethylene glycol dimethyl ether 250 22 v/v -
  • Ammonium sulfate 0.087 M -
  • tris chloride BCC 0.5 M pH 7
pH 7
24.9 μl of Ammonium sulfate (3.5 M)
440.0 μl of Polyethylene glycol dimethyl ether 250 (50 v/v)
500.0 μl of tris chloride BCC pH 7 (1 M)
35.1 μl of water (100 v/v)
C5
  • Polyethylene glycol 8,000 13 w/v -
  • Magnesium chloride 0.1 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
50.0 μl of Magnesium chloride (2 M)
260.0 μl of Polyethylene glycol 8,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
590.0 μl of water (100 v/v)
C6
  • Polyethylene glycol 3,350 17 w/v -
  • Magnesium formate 0.1 M -
  • MOPS 0.1 M pH 7
pH 7
67.0 μl of MOPS pH 6.5 (1 M)
33.0 μl of MOPS pH 7.9 (1 M)
100.0 μl of Magnesium formate (1 M)
340.0 μl of Polyethylene glycol 3,350 (50 w/v)
460.0 μl of water (100 v/v)
C7
  • Polyethylene glycol 4,000 18 w/v -
  • Potassium chloride 0.1 M -
  • BIS-TRIS 0.1 M pH 6
pH 6
63.5 μl of BIS-TRIS pH 5.5 (1 M)
36.5 μl of BIS-TRIS pH 6.5 (1 M)
360.0 μl of Polyethylene glycol 4,000 (50 w/v)
25.0 μl of Potassium chloride (4 M)
515.0 μl of water (100 v/v)
C8
  • Polyethylene glycol 200 18 v/v -
  • Potassium chloride 0.1 M -
  • Potassium di-hydrogen phosphate 0.1 M pH 7.5
pH 7.5
180.0 μl of Polyethylene glycol 200 (100 v/v)
25.0 μl of Potassium chloride (4 M)
50.0 μl of Potassium di-hydrogen phosphate pH 7.5 (2 M)
745.0 μl of water (100 v/v)
C9
  • Polyethylene glycol 4,000 22 w/v -
  • Magnesium acetate 0.1 M -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
100.0 μl of Magnesium acetate (1 M)
440.0 μl of Polyethylene glycol 4,000 (50 w/v)
260.0 μl of water (100 v/v)
C10
  • Polyethylene glycol 8,000 22 w/v -
  • Calcium acetate 0.1 M -
  • MES 0.1 M pH 6
pH 6
50.0 μl of Calcium acetate (2 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
440.0 μl of Polyethylene glycol 8,000 (50 w/v)
310.0 μl of water (100 v/v)
C11
  • Polyethylene glycol 3,350 23 w/v -
  • Ammonium sulfate 0.1 M -
  • HEPES 0.1 M pH 8.5
pH 8.5
28.6 μl of Ammonium sulfate (3.5 M)
100.0 μl of HEPES pH 8.5 (1 M)
460.0 μl of Polyethylene glycol 3,350 (50 w/v)
411.4 μl of water (100 v/v)
C12
  • Polyethylene glycol 400 32 v/v -
  • Potassium chloride 0.1 M -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
25.0 μl of Potassium chloride (4 M)
455.0 μl of water (100 v/v)
D1
  • Polyethylene glycol 300 36 v/v -
  • Sodium chloride 0.1 M -
  • MES 0.1 M pH 6.5
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
360.0 μl of Polyethylene glycol 300 (100 v/v)
20.0 μl of Sodium chloride (5 M)
420.0 μl of water (100 v/v)
D2
  • Polyethylene glycol monomethyl ether 550 45 v/v -
  • Sodium chloride 0.1 M -
  • BICINE 0.1 M pH 9
pH 9
9.5 μl of BICINE pH 7.4 (1 M)
90.5 μl of BICINE pH 9.3 (1 M)
450.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
20.0 μl of Sodium chloride (5 M)
430.0 μl of water (100 v/v)
D3
  • Polyethylene glycol 400 35 v/v -
  • Calcium chloride 0.15 M -
  • Glycine 0.1 M pH 9
pH 9
75.0 μl of Calcium chloride (2 M)
100.0 μl of Glycine pH 9 (1 M)
350.0 μl of Polyethylene glycol 400 (100 v/v)
475.0 μl of water (100 v/v)
D4
  • Polyethylene glycol 4,000 13 w/v -
  • Ammonium sulfate 0.2 M -
  • ADA 0.5 M pH 6.5
pH 6.5
500.0 μl of ADA pH 6.5 (1 M)
57.1 μl of Ammonium sulfate (3.5 M)
260.0 μl of Polyethylene glycol 4,000 (50 w/v)
182.9 μl of water (100 v/v)
D5
  • Polyethylene glycol monomethyl ether 2,000 14 w/v -
  • Choline chloride 0.2 M -
  • tris chloride BCC 0.1 M pH 7.5
pH 7.5
200.0 μl of Choline chloride (1 M)
280.0 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
100.0 μl of tris chloride BCC pH 7.5 (1 M)
420.0 μl of water (100 v/v)
D6
  • Polyethylene glycol 6,000 19 w/v -
  • Sodium chloride 0.2 M -
  • MOPS 0.05 M pH 7
pH 7
33.5 μl of MOPS pH 6.5 (1 M)
16.5 μl of MOPS pH 7.9 (1 M)
380.0 μl of Polyethylene glycol 6,000 (50 w/v)
40.0 μl of Sodium chloride (5 M)
530.0 μl of water (100 v/v)
D7
  • Polyethylene glycol 6,000 19 w/v -
  • Sodium chloride 0.05 M -
  • MOPS 0.05 M pH 7
pH 7
33.5 μl of MOPS pH 6.5 (1 M)
16.5 μl of MOPS pH 7.9 (1 M)
380.0 μl of Polyethylene glycol 6,000 (50 w/v)
10.0 μl of Sodium chloride (5 M)
560.0 μl of water (100 v/v)
D8
  • Polyethylene glycol 3,350 19 w/v -
  • Magnesium formate 0.2 M -
  • tris chloride BCC 0.05 M pH 8
pH 8
200.0 μl of Magnesium formate (1 M)
380.0 μl of Polyethylene glycol 3,350 (50 w/v)
50.0 μl of tris chloride BCC pH 8 (1 M)
370.0 μl of water (100 v/v)
D9
  • Polyethylene glycol monomethyl ether 350 20 v/v -
  • Calcium chloride 0.2 M -
  • MES 0.1 M pH 5
pH 5
100.0 μl of Calcium chloride (2 M)
100.0 μl of MES pH 5 (1 M)
200.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
600.0 μl of water (100 v/v)
D10
  • Polyethylene glycol 3,350 20 w/v -
  • Ammonium nitrate 0.2 M -
  • HEPES 0.05 M pH 7
pH 7
100.0 μl of Ammonium nitrate (2 M)
50.0 μl of HEPES pH 7 (1 M)
400.0 μl of Polyethylene glycol 3,350 (50 w/v)
450.0 μl of water (100 v/v)
D11
  • Polyethylene glycol 1,000 33 w/v -
  • Magnesium chloride 0.5 M -
  • Lithium chloride 0.02 M -
  • Glycine 0.02 M pH 10
pH 10
20.0 μl of Glycine pH 10 (1 M)
2.0 μl of Lithium chloride (10 M)
250.0 μl of Magnesium chloride (2 M)
660.0 μl of Polyethylene glycol 1,000 (50 w/v)
68.0 μl of water (100 v/v)
D12
  • Polyethylene glycol 400 24 v/v -
  • Calcium acetate 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of Calcium acetate (2 M)
100.0 μl of HEPES pH 7 (1 M)
240.0 μl of Polyethylene glycol 400 (100 v/v)
560.0 μl of water (100 v/v)
E1
  • Polyethylene glycol 400 28 v/v -
  • Sodium acetate 0.2 M -
  • MES 0.1 M pH 6.5
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
280.0 μl of Polyethylene glycol 400 (100 v/v)
66.7 μl of Sodium acetate (3 M)
453.3 μl of water (100 v/v)
E2
  • Polyethylene glycol 400 29 v/v -
  • Sodium chloride 0.2 M -
  • Calcium acetate 0.05 M pH 5
pH 5
50.0 μl of Calcium acetate pH 5 (1 M)
290.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium chloride (5 M)
620.0 μl of water (100 v/v)
E3
  • Polyethylene glycol 400 29 v/v -
  • Sodium chloride 0.2 M -
  • HEPES 0.1 M pH 7
pH 7
100.0 μl of HEPES pH 7 (1 M)
290.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium chloride (5 M)
570.0 μl of water (100 v/v)
E4
  • Pentaerythritol ethoxylate (15/4 EO/OH) 31 v/v -
  • Ammonium formate 0.2 M -
  • tris chloride BCC 0.1 M pH 7
pH 7
20.0 μl of Ammonium formate (10 M)
620.0 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
100.0 μl of tris chloride BCC pH 7 (1 M)
260.0 μl of water (100 v/v)
E5
  • Polyethylene glycol 3,350 35 w/v -
  • Ammonium sulfate 0.2 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
57.1 μl of Ammonium sulfate (3.5 M)
700.0 μl of Polyethylene glycol 3,350 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
142.9 μl of water (100 v/v)
E6
  • Polyethylene glycol 400 38 v/v -
  • Calcium acetate 0.2 M -
  • Sodium acetate 0.1 M pH 5
pH 5
100.0 μl of Calcium acetate (2 M)
380.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Sodium acetate pH 5 (1 M)
420.0 μl of water (100 v/v)
E7
  • Polyethylene glycol 400 38 v/v -
  • Sodium chloride 0.2 M -
  • MOPS 0.1 M pH 7.5
pH 7.5
25.1 μl of MOPS pH 6.5 (1 M)
74.9 μl of MOPS pH 7.9 (1 M)
380.0 μl of Polyethylene glycol 400 (100 v/v)
40.0 μl of Sodium chloride (5 M)
480.0 μl of water (100 v/v)
E8
  • Ammonium sulfate 2 M -
  • Sodium chloride 0.2 M -
  • MES 0.1 M pH 6.5
pH 6.5
571.4 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 6.5 (1 M)
40.0 μl of Sodium chloride (5 M)
288.6 μl of water (100 v/v)
E9
  • Polyethylene glycol 4,000 12 w/v -
  • Ammonium sulfate 0.225 M -
  • Sodium acetate 0.05 M pH 4
pH 4
64.3 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
50.0 μl of Sodium acetate pH 4 (1 M)
645.7 μl of water (100 v/v)
E10
  • Polyethylene glycol 400 33 v/v -
  • Sodium chloride 0.23 M -
  • Sodium acetate 0.05 M pH 4.5
pH 4.5
330.0 μl of Polyethylene glycol 400 (100 v/v)
29.3 μl of Sodium acetate pH 4 (1 M)
20.7 μl of Sodium acetate pH 5 (1 M)
46.0 μl of Sodium chloride (5 M)
574.0 μl of water (100 v/v)
E11
  • Polyethylene glycol 3,000 22 w/v -
  • Magnesium formate 0.25 M -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
250.0 μl of Magnesium formate (1 M)
440.0 μl of Polyethylene glycol 3,000 (50 w/v)
210.0 μl of water (100 v/v)
E12
  • Polyethylene glycol 1,000 40 w/v -
  • Magnesium chloride 0.25 M -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
62.5 μl of Magnesium chloride (4 M)
800.0 μl of Polyethylene glycol 1,000 (50 w/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
37.5 μl of water (100 v/v)
F1
  • Polyethylene glycol 400 25 v/v -
  • Lithium sulfate 0.3 M -
  • MES 0.1 M pH 6.5
pH 6.5
150.0 μl of Lithium sulfate (2 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
250.0 μl of Polyethylene glycol 400 (100 v/v)
400.0 μl of water (100 v/v)
F2
  • Polyethylene glycol monomethyl ether 550 33 v/v -
  • Ammonium formate 0.3 M -
  • tris chloride BCC 0.05 M pH 9
pH 9
30.0 μl of Ammonium formate (10 M)
330.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
50.0 μl of tris chloride BCC pH 9 (1 M)
590.0 μl of water (100 v/v)
F3
  • Polyethylene glycol 400 34 v/v -
  • Barium chloride 0.3 M -
  • MES 0.1 M pH 6
pH 6
300.0 μl of Barium chloride (1 M)
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
340.0 μl of Polyethylene glycol 400 (100 v/v)
160.0 μl of water (100 v/v)
F4
  • Polyethylene glycol 4,000 14 w/v -
  • Lithium chloride 0.32 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
32.0 μl of Lithium chloride (10 M)
280.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
625.5 μl of water (100 v/v)
F5
  • Polyethylene glycol 4,000 12 w/v -
  • Ammonium sulfate 0.34 M -
  • tri-Sodium citrate 0.1 M pH 5.5
pH 5.5
97.1 μl of Ammonium sulfate (3.5 M)
240.0 μl of Polyethylene glycol 4,000 (50 w/v)
22.7 μl of tri-Sodium citrate pH 3 (1.6 M)
39.8 μl of tri-Sodium citrate pH 6.5 (1.6 M)
600.4 μl of water (100 v/v)
F6
  • Polyethylene glycol 600 11 v/v -
  • Lithium sulfate 0.35 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
175.0 μl of Lithium sulfate (2 M)
110.0 μl of Polyethylene glycol 600 (100 v/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
615.0 μl of water (100 v/v)
F7
  • Polyethylene glycol 400 22 v/v -
  • Potassium nitrate 0.37 M -
NA
220.0 μl of Polyethylene glycol 400 (100 v/v)
123.3 μl of Potassium nitrate (3 M)
656.7 μl of water (100 v/v)
F8
  • Polyethylene glycol 3,350 10 w/v -
  • Ammonium sulfate 0.4 M -
  • MES 0.1 M pH 6.5
pH 6.5
114.3 μl of Ammonium sulfate (3.5 M)
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
200.0 μl of Polyethylene glycol 3,350 (50 w/v)
485.7 μl of water (100 v/v)
F9
  • Polyethylene glycol 400 32 v/v -
  • Sodium chloride 0.05 M -
  • Magnesium chloride 0.04 M -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
20.0 μl of Magnesium chloride (2 M)
320.0 μl of Polyethylene glycol 400 (100 v/v)
10.0 μl of Sodium chloride (5 M)
550.0 μl of water (100 v/v)
F10
  • Polyethylene glycol 400 12 v/v -
  • Potassium chloride 0.4 M -
  • HEPES 0.05 M pH 7.5
pH 7.5
50.0 μl of HEPES pH 7.5 (1 M)
120.0 μl of Polyethylene glycol 400 (100 v/v)
100.0 μl of Potassium chloride (4 M)
730.0 μl of water (100 v/v)
F11
  • Polyethylene glycol 4,000 15 w/v -
  • Ammonium thiocyanate 0.4 M -
  • Sodium acetate 0.1 M pH 4.5
pH 4.5
400.0 μl of Ammonium thiocyanate (1 M)
300.0 μl of Polyethylene glycol 4,000 (50 w/v)
58.6 μl of Sodium acetate pH 4 (1 M)
41.4 μl of Sodium acetate pH 5 (1 M)
200.0 μl of water (100 v/v)
F12
  • Polyethylene glycol 4,000 16 w/v -
  • Sodium thiocyanate 0.4 M -
  • Sodium acetate 0.1 M pH 4
pH 4
320.0 μl of Polyethylene glycol 4,000 (50 w/v)
100.0 μl of Sodium acetate pH 4 (1 M)
50.0 μl of Sodium thiocyanate (8 M)
530.0 μl of water (100 v/v)
G1
  • Polyethylene glycol 400 20 v/v -
  • Potassium chloride 0.5 M -
  • HEPES 0.05 M pH 6.5
pH 6.5
31.5 μl of HEPES pH 5 (1 M)
18.5 μl of HEPES pH 7 (1 M)
200.0 μl of Polyethylene glycol 400 (100 v/v)
125.0 μl of Potassium chloride (4 M)
625.0 μl of water (100 v/v)
G2
  • Polyethylene glycol monomethyl ether 350 21 v/v -
  • Magnesium chloride 0.5 M -
  • tris chloride BCC 0.05 M pH 7.5
pH 7.5
250.0 μl of Magnesium chloride (2 M)
210.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
490.0 μl of water (100 v/v)
G3
  • Polyethylene glycol 4,000 11 w/v -
  • Potassium formate 0.8 M -
  • Sodium acetate 0.1 M pH 5
pH 5
220.0 μl of Polyethylene glycol 4,000 (50 w/v)
57.1 μl of Potassium formate (14 M)
100.0 μl of Sodium acetate pH 5 (1 M)
622.9 μl of water (100 v/v)
G4
  • Polyethylene glycol 8,000 9 w/v -
  • MOPS 0.1 M pH 7
pH 7
67.0 μl of MOPS pH 6.5 (1 M)
33.0 μl of MOPS pH 7.9 (1 M)
180.0 μl of Polyethylene glycol 8,000 (50 w/v)
720.0 μl of water (100 v/v)
G5
  • Polyethylene glycol 20,000 11 w/v -
  • MES 0.1 M pH 6
pH 6
117.0 μl of MES pH 5.2 (0.5 M)
83.0 μl of MES pH 7.1 (0.5 M)
366.7 μl of Polyethylene glycol 20,000 (30 w/v)
433.3 μl of water (100 v/v)
G6
  • Polyethylene glycol 400 13 v/v -
  • MES 0.1 M pH 6.5
pH 6.5
48.6 μl of MES pH 5.2 (0.5 M)
151.4 μl of MES pH 7.1 (0.5 M)
130.0 μl of Polyethylene glycol 400 (100 v/v)
670.0 μl of water (100 v/v)
G7
  • Polyethylene glycol 6,000 14 w/v -
  • ADA 0.1 M pH 5.5
pH 5.5
100.0 μl of ADA pH 5.5 (1 M)
280.0 μl of Polyethylene glycol 6,000 (50 w/v)
620.0 μl of water (100 v/v)
G8
  • Polyethylene glycol monomethyl ether 350 17 v/v -
  • tris chloride BCC 0.05 M pH 7.5
pH 7.5
170.0 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
50.0 μl of tris chloride BCC pH 7.5 (1 M)
780.0 μl of water (100 v/v)
G9
  • Polyethylene glycol 300 22 v/v -
  • tri-Sodium citrate 0.07 M pH 4.5
pH 4.5
220.0 μl of Polyethylene glycol 300 (100 v/v)
24.7 μl of tri-Sodium citrate pH 3 (1.6 M)
19.0 μl of tri-Sodium citrate pH 6.5 (1.6 M)
736.2 μl of water (100 v/v)
G10
  • Polyethylene glycol 400 24 v/v -
  • ADA 0.05 M pH 6.5
pH 6.5
50.0 μl of ADA pH 6.5 (1 M)
240.0 μl of Polyethylene glycol 400 (100 v/v)
710.0 μl of water (100 v/v)
G11
  • Polyethylene glycol 1,500 24 w/v -
  • MES 0.1 M pH 6.5
pH 6.5
100.0 μl of MES pH 6.5 (1 M)
480.0 μl of Polyethylene glycol 1,500 (50 w/v)
420.0 μl of water (100 v/v)
G12
  • Polyethylene glycol 600 28 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
280.0 μl of Polyethylene glycol 600 (100 v/v)
620.0 μl of water (100 v/v)
H1
  • Polyethylene glycol 400 28 v/v -
  • tris chloride BCC 0.05 M pH 8.5
pH 8.5
280.0 μl of Polyethylene glycol 400 (100 v/v)
50.0 μl of tris chloride BCC pH 8.5 (1 M)
670.0 μl of water (100 v/v)
H2
  • Polyethylene glycol 400 30 v/v -
  • BICINE 0.1 M pH 9
pH 9
9.5 μl of BICINE pH 7.4 (1 M)
90.5 μl of BICINE pH 9.3 (1 M)
300.0 μl of Polyethylene glycol 400 (100 v/v)
600.0 μl of water (100 v/v)
H3
  • Polyethylene glycol 600 31 v/v -
  • ADA 0.1 M pH 7
pH 7
100.0 μl of ADA pH 7 (1 M)
310.0 μl of Polyethylene glycol 600 (100 v/v)
590.0 μl of water (100 v/v)
H4
  • Polyethylene glycol monomethyl ether 550 32 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
320.0 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
580.0 μl of water (100 v/v)
H5
  • Polyethylene glycol 400 33 v/v -
  • HEPES 0.1 M pH 7.5
pH 7.5
100.0 μl of HEPES pH 7.5 (1 M)
330.0 μl of Polyethylene glycol 400 (100 v/v)
570.0 μl of water (100 v/v)
H6
  • Polyethylene glycol 3,350 34 w/v -
  • tri-Sodium citrate 0.18 M pH 4
pH 4
680.0 μl of Polyethylene glycol 3,350 (50 w/v)
70.8 μl of tri-Sodium citrate pH 3 (1.6 M)
41.7 μl of tri-Sodium citrate pH 6.5 (1.6 M)
207.5 μl of water (100 v/v)
H7
  • Polyethylene glycol 200 44 v/v -
  • tris chloride BCC 0.1 M pH 8.5
pH 8.5
440.0 μl of Polyethylene glycol 200 (100 v/v)
100.0 μl of tris chloride BCC pH 8.5 (1 M)
460.0 μl of water (100 v/v)
H8
  • 2-methyl-2,4-pentanediol BCC 65 v/v -
  • tris chloride BCC 0.1 M pH 8
pH 8
650.0 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
100.0 μl of tris chloride BCC pH 8 (1 M)
250.0 μl of water (100 v/v)
H9
  • Ammonium chloride 2.75 M -
  • BIS-TRIS 0.025 M pH 7
pH 7
550.0 μl of Ammonium chloride (5 M)
25.0 μl of BIS-TRIS pH 7 (1 M)
425.0 μl of water (100 v/v)
H10
  • Ammonium chloride 2.8 M -
  • HEPES 0.075 M pH 7.5
pH 7.5
560.0 μl of Ammonium chloride (5 M)
75.0 μl of HEPES pH 7.5 (1 M)
365.0 μl of water (100 v/v)
H11
  • Ammonium sulfate 3 M -
  • MES 0.1 M pH 5.5
pH 5.5
857.1 μl of Ammonium sulfate (3.5 M)
100.0 μl of MES pH 5.5 (1 M)
42.9 μl of water (100 v/v)
H12
  • 1,6-Hexanediol 3.25 M -
  • HEPES 0.01 M pH 7.5
pH 7.5
541.7 μl of 1,6-Hexanediol (6 M)
10.0 μl of HEPES pH 7.5 (1 M)
448.3 μl of water (100 v/v)