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Supplemental Information

Screen Description

MemChannel Eco

Molecular Dimensions / MemChannel Eco
Created 2020-05-11.

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MemChannel Eco

    Screen Structure

    MemChannel Eco

    Well Count 96
    Number of distinct ingredients 64
    Internal diversity (score mean dist) 0.933

    Screen Composition

    2025-06-15T02:24:58.764356 image/svg+xml Matplotlib v3.10.3, https://matplotlib.org/
    ingredient type MemChannel Eco
    Buffer 116
    Organic (non-volatile) 43
    Polymer 102
    Salt 124

    Well Contents

    Buffers are shown in italics. More information about buffers.
    Primary factors are shown in bold. More information about primary factors.
    MemChannel Eco Well Contents  🔍
    WellIngredient, Concentration, Units, pHpHRecipe 1000μl
    A1
    • Lithium Phosphate 0.05 M -
    • Sodium acetate 0.025 M pH 5
    • Polyethylene glycol 300 23 v/v -
    pH 5
    17 μl of Sodium acetate pH 5.5 (1 M)
    230 μl of Polyethylene glycol 300 (100 v/v)
    50 μl of Lithium Phosphate (1 M)
    7 μl of Sodium acetate pH 4 (1 M)
    695 μl of water (100 v/v)
    A2
    • Potassium thiocyanate 0.3 M -
    • CHES 0.1 M pH 9
    • Polyethylene glycol 3,350 15 w/v -
    pH 9
    100 μl of CHES pH 9 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    37 μl of Potassium thiocyanate (8 M)
    562 μl of water (100 v/v)
    A3
    • Sodium chloride 0.1 M -
    • HEPES 0.05 M pH 7.5
    • Polyethylene glycol 400 25 v/v -
    pH 7.5
    20 μl of Sodium chloride (5 M)
    25 μl of HEPES pH 7 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    25 μl of HEPES pH 8 (1 M)
    680 μl of water (100 v/v)
    A4
    • Sodium chloride 1 M -
    • tri-Sodium citrate 0.05 M pH 6
    • Polyethylene glycol 400 32 v/v -
    pH 6
    200 μl of Sodium chloride (5 M)
    48 μl of tri-Sodium citrate pH 7 (1 M)
    1 μl of tri-Sodium citrate pH 5.6 (1 M)
    320 μl of Polyethylene glycol 400 (100 v/v)
    430 μl of water (100 v/v)
    A5
    • Potassium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 30 v/v -
    pH 8
    54 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Potassium chloride (1 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    300 μl of Polyethylene glycol 400 (100 v/v)
    500 μl of water (100 v/v)
    A6
    • Magnesium chloride 0.2 M -
    • MES 0.05 M pH 6.5
    • Polyethylene glycol 4,000 6 w/v -
    pH 6.5
    50 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5.5 (1 M)
    120 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Magnesium chloride (2 M)
    730 μl of water (100 v/v)
    A7
    • Potassium nitrate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 3,350 17 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5.5 (1 M)
    200 μl of Potassium nitrate (1 M)
    340 μl of Polyethylene glycol 3,350 (50 w/v)
    360 μl of water (100 v/v)
    A8
    • SPG buffer 0.1 M pH 8.1
    • Polyethylene glycol 1,500 20 w/v -
    pH 8.1
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    99 μl of SPG buffer pH 9 (1 M)
    0 μl of SPG buffer pH 8 (1 M)
    500 μl of water (100 v/v)
    A9
    • Sodium chloride 0.1 M -
    • Magnesium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 20 v/v -
    pH 7.5
    100 μl of Magnesium chloride (1 M)
    400 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    100 μl of Sodium chloride (1 M)
    50 μl of HEPES pH 7 (1 M)
    50 μl of HEPES pH 8 (1 M)
    300 μl of water (100 v/v)
    A10
    • Zinc sulfate 0.2 M -
    • Sodium acetate 0.05 M pH 4.4
    • Polyethylene glycol 4,000 15 w/v -
    pH 4.4
    200 μl of Zinc sulfate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    10 μl of Sodium acetate pH 5.5 (1 M)
    39 μl of Sodium acetate pH 4 (1 M)
    450 μl of water (100 v/v)
    A11
    • Sodium thiocyanate 0.4 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol 4,000 8 w/v -
    pH 4
    200 μl of Sodium thiocyanate (2 M)
    160 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    540 μl of water (100 v/v)
    A12
    • tri-Sodium citrate 0.05 M pH 5.6
    • Polyethylene glycol 2,000 10 w/v -
    pH 5.6
    47 μl of tri-Sodium citrate pH 6.5 (1 M)
    2 μl of tri-Sodium citrate pH 3.1 (1 M)
    200 μl of Polyethylene glycol 2,000 (50 w/v)
    750 μl of water (100 v/v)
    B1
    • Ammonium sulfate 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 400 22 v/v -
    pH 7.5
    440 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of HEPES pH 7 (1 M)
    100 μl of Ammonium sulfate (1 M)
    50 μl of HEPES pH 8 (1 M)
    360 μl of water (100 v/v)
    B2
    • MES 0.1 M pH 6.5
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5.5 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    600 μl of water (100 v/v)
    B3
    • Ammonium di-hydrogen phosphate 0.2 M -
    • BIS-TRIS 0.1 M pH 6.7
    • Polyethylene glycol 1,500 20 w/v -
    pH 6.7
    28 μl of BIS-TRIS pH 6 (1 M)
    71 μl of BIS-TRIS pH 7 (1 M)
    400 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Ammonium di-hydrogen phosphate (1 M)
    300 μl of water (100 v/v)
    B4
    • Ammonium chloride 0.2 M -
    • MOPS 0.05 M pH 7.5
    • Polyethylene glycol monomethyl ether 2,000 17 w/v -
    pH 7.5
    0 μl of MOPS pH 6.5 (1 M)
    50 μl of MOPS pH 7.5 (1 M)
    340 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    200 μl of Ammonium chloride pH 6.3 (1 M)
    410 μl of water (100 v/v)
    B5
    • Magnesium chloride 0.01 M -
    • Magnesium acetate 0.045 M -
    • HEPES 0.08 M pH 7.5
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 4,000 2.5 w/v -
    • Polyethylene glycol 8,000 2.5 w/v -
    pH 7.5
    10 μl of Magnesium chloride (1 M)
    50 μl of Polyethylene glycol 8,000 (50 w/v)
    45 μl of Magnesium acetate (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of HEPES pH 7 (1 M)
    50 μl of Polyethylene glycol 4,000 (50 w/v)
    40 μl of HEPES pH 8 (1 M)
    365 μl of water (100 v/v)
    B6
    • di-Sodium malonate 0.2 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 20 w/v -
    pH 7
    200 μl of di-Sodium malonate pH 7 (1 M)
    400 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of HEPES pH 7 (1 M)
    0 μl of HEPES pH 8 (1 M)
    300 μl of water (100 v/v)
    B7
    • Ammonium sulfate 0.2 M -
    • BIS-TRIS 0.05 M pH 6.7
    • Polyethylene glycol 400 25 v/v -
    pH 6.7
    14 μl of BIS-TRIS pH 6 (1 M)
    35 μl of BIS-TRIS pH 7 (1 M)
    500 μl of Polyethylene glycol 400 (50 v/v)
    200 μl of Ammonium sulfate (1 M)
    250 μl of water (100 v/v)
    B8
    • Nickel sulfate 0.05 M -
    • tris chloride BCC 0.05 M pH 8.8
    • Polyethylene glycol 400 30 v/v -
    pH 8.8
    3 μl of tris chloride BCC pH 7 (1 M)
    600 μl of Polyethylene glycol 400 (50 v/v)
    46 μl of tris chloride BCC pH 9 (1 M)
    12 μl of Nickel sulfate (4 M)
    337 μl of water (100 v/v)
    B9
    • Ammonium sulfate 0.2 M -
    • Sodium chloride 0.02 M -
    • tri-Sodium citrate 0.1 M pH 3.7
    • Polyethylene glycol 400 19 v/v -
    pH 3.7
    80 μl of tri-Sodium citrate pH 6.5 (1 M)
    19 μl of tri-Sodium citrate pH 3.1 (1 M)
    380 μl of Polyethylene glycol 400 (50 v/v)
    20 μl of Sodium chloride (1 M)
    200 μl of Ammonium sulfate (1 M)
    300 μl of water (100 v/v)
    B10
    • Magnesium acetate 0.075 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 1,500 22 w/v -
    • 2-methyl-2,4-pentanediol BCC 2 v/v -
    pH 8.5
    133 μl of 2-methyl-2,4-pentanediol BCC (15 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    75 μl of Magnesium acetate (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    251 μl of water (100 v/v)
    B11
    • Sodium chloride 0.1 M -
    • CHES 0.1 M pH 9
    • Polyethylene glycol 600 16 v/v -
    pH 9
    100 μl of CHES pH 9 (1 M)
    100 μl of Sodium chloride (1 M)
    160 μl of Polyethylene glycol 600 (100 v/v)
    640 μl of water (100 v/v)
    B12
    • Magnesium chloride 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 4,000 11 w/v -
    pH 5
    100 μl of Magnesium chloride (1 M)
    90 μl of tri-Sodium citrate pH 6.5 (1 M)
    9 μl of tri-Sodium citrate pH 3.1 (1 M)
    220 μl of Polyethylene glycol 4,000 (50 w/v)
    580 μl of water (100 v/v)
    C1
    • Zinc chloride 0.12 M -
    • tri-Potassium citrate 0.1 M pH 4
    • Polyethylene glycol 300 42 v/v -
    pH 4
    120 μl of Zinc chloride (1 M)
    100 μl of tri-Potassium citrate pH 4 (1 M)
    420 μl of Polyethylene glycol 300 (100 v/v)
    360 μl of water (100 v/v)
    C2
    • Calcium chloride 0.1 M -
    • MES 0.05 M pH 5.5
    • Polyethylene glycol 400 29 v/v -
    pH 5.5
    50 μl of MES pH 5.5 (1 M)
    100 μl of Calcium chloride (1 M)
    290 μl of Polyethylene glycol 400 (100 v/v)
    560 μl of water (100 v/v)
    C3
    • Magnesium nitrate 0.1 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol monomethyl ether 2,000 15 w/v -
    pH 8
    54 μl of tris chloride BCC pH 7 (1 M)
    300 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    45 μl of tris chloride BCC pH 9 (1 M)
    100 μl of Magnesium nitrate (1 M)
    500 μl of water (100 v/v)
    C4
    • Potassium thiocyanate 0.2 M -
    • Calcium chloride 0.01 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 4,000 14 w/v -
    pH 7.5
    50 μl of HEPES pH 7 (1 M)
    280 μl of Polyethylene glycol 4,000 (50 w/v)
    10 μl of Calcium chloride (1 M)
    25 μl of Potassium thiocyanate (8 M)
    50 μl of HEPES pH 8 (1 M)
    585 μl of water (100 v/v)
    C5
    • ADA 0.05 M pH 7
    • Polyethylene glycol 1,500 10 w/v -
    • Polyethylene glycol 1,000 10 w/v -
    pH 7
    200 μl of Polyethylene glycol 1,500 (50 w/v)
    200 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of ADA pH 7 (1 M)
    550 μl of water (100 v/v)
    C6
    • HEPES 0.1 M pH 7
    • 2-methyl-2,4-pentanediol BCC 65 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    650 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    250 μl of water (100 v/v)
    C7
    • Lithium nitrate 0.125 M -
    • Glycine 0.1 M pH 9.8
    • Polyethylene glycol 400 45 v/v -
    pH 9.8
    15 μl of Lithium nitrate (8 M)
    23 μl of Glycine pH 9 (1 M)
    76 μl of Glycine pH 10 (1 M)
    450 μl of Polyethylene glycol 400 (100 v/v)
    434 μl of water (100 v/v)
    C8
    • Sodium chloride 0.1 M -
    • Ammonium sulfate 0.2 M -
    • tri-Sodium citrate 0.1 M pH 6
    • Polyethylene glycol 400 10 v/v -
    • Polyethylene glycol 2,000 18 w/v -
    pH 6
    97 μl of tri-Sodium citrate pH 6.5 (1 M)
    2 μl of tri-Sodium citrate pH 3.1 (1 M)
    100 μl of Sodium chloride (1 M)
    200 μl of Ammonium sulfate (1 M)
    100 μl of Polyethylene glycol 400 (100 v/v)
    360 μl of Polyethylene glycol 2,000 (50 w/v)
    140 μl of water (100 v/v)
    C9
    • Lithium sulfate 0.2 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 20 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5.5 (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    200 μl of Lithium sulfate (1 M)
    300 μl of water (100 v/v)
    C10
    • Ammonium sulfate 0.27 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 3,350 15 w/v -
    pH 9
    100 μl of BICINE pH 9 (1 M)
    270 μl of Ammonium sulfate (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    330 μl of water (100 v/v)
    C11
    • Sodium chloride 0.125 M -
    • Lithium sulfate 0.16 M -
    • HEPES 0.075 M pH 8
    • Polyethylene glycol 300 14 v/v -
    pH 8
    125 μl of Sodium chloride (1 M)
    160 μl of Lithium sulfate (1 M)
    140 μl of Polyethylene glycol 300 (100 v/v)
    75 μl of HEPES pH 8 (1 M)
    500 μl of water (100 v/v)
    C12
    • tri-Sodium citrate 0.05 M -
    • tri-Potassium citrate 0.05 M -
    • ADA 0.1 M pH 7
    • Polyethylene glycol 400 12 v/v -
    pH 7
    240 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of tri-Sodium citrate (1 M)
    33 μl of tri-Potassium citrate (1.5 M)
    100 μl of ADA pH 7 (1 M)
    576 μl of water (100 v/v)
    D1
    • Sodium chloride 0.5 M -
    • Calcium chloride 0.05 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 38 v/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    100 μl of Sodium chloride (5 M)
    0 μl of MES pH 5.5 (1 M)
    50 μl of Calcium chloride (1 M)
    380 μl of Polyethylene glycol 400 (100 v/v)
    370 μl of water (100 v/v)
    D2
    • tri-Sodium citrate 0.05 M -
    • Potassium di-hydrogen phosphate 0.2 M -
    • BIS-TRIS 0.08 M pH 6
    • Polyethylene glycol 4,000 10 w/v -
    pH 6
    200 μl of Potassium di-hydrogen phosphate (1 M)
    80 μl of BIS-TRIS pH 6 (1 M)
    0 μl of BIS-TRIS pH 7 (1 M)
    50 μl of tri-Sodium citrate (1 M)
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    470 μl of water (100 v/v)
    D3
    • Lithium sulfate 0.05 M -
    • Sodium di-hydrogen phosphate 0.05 M -
    • tri-Sodium citrate 0.08 M pH 4.5
    • Polyethylene glycol 1,000 15 w/v -
    pH 4.5
    300 μl of Polyethylene glycol 1,000 (50 w/v)
    50 μl of Lithium sulfate (1 M)
    16 μl of Sodium di-hydrogen phosphate (3 M)
    80 μl of tri-Sodium citrate pH 4.5 (1 M)
    553 μl of water (100 v/v)
    D4
    • Potassium di-hydrogen phosphate 0.1 M -
    • ADA 0.1 M pH 6.5
    • Polyethylene glycol 400 35 v/v -
    pH 6.5
    100 μl of ADA pH 6.5 (1 M)
    100 μl of Potassium di-hydrogen phosphate pH 7 (1 M)
    350 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    D5
    • di-Sodium succinate 0.1 M pH 7
    • Polyethylene glycol 3,350 15 w/v -
    pH 7
    100 μl of di-Sodium succinate pH 7 (1 M)
    300 μl of Polyethylene glycol 3,350 (50 w/v)
    600 μl of water (100 v/v)
    D6
    • Sodium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 10,000 23 w/v -
    pH 8.5
    100 μl of Sodium sulfate (1 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    460 μl of Polyethylene glycol 10,000 (50 w/v)
    78 μl of tris chloride BCC pH 9 (1 M)
    340 μl of water (100 v/v)
    D7
    • Lithium sulfate 0.1 M -
    • MOPS 0.1 M pH 7.5
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    • Polyethylene glycol 20,000 10 w/v -
    pH 7.5
    100 μl of MOPS pH 7.5 (1 M)
    333 μl of Polyethylene glycol 20,000 (30 w/v)
    200 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    100 μl of Lithium sulfate (1 M)
    266 μl of water (100 v/v)
    D8
    • Magnesium chloride 0.3 M -
    • BICINE 0.1 M pH 9
    • Polyethylene glycol 2,000 24 w/v -
    pH 9
    300 μl of Magnesium chloride pH 7 (1 M)
    100 μl of BICINE pH 9 (1 M)
    480 μl of Polyethylene glycol 2,000 (50 w/v)
    120 μl of water (100 v/v)
    D9
    • Sodium chloride 0.1 M -
    • Ammonium formate 0.05 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol monomethyl ether 350 22 v/v -
    pH 8.5
    220 μl of Polyethylene glycol monomethyl ether 350 (100 v/v)
    21 μl of tris chloride BCC pH 7 (1 M)
    100 μl of Sodium chloride (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    20 μl of Ammonium formate (2.5 M)
    560 μl of water (100 v/v)
    D10
    • Calcium chloride 0.1 M -
    • HEPES 0.1 M pH 7.5
    • Polyethylene glycol 8,000 5 w/v -
    pH 7.5
    100 μl of Polyethylene glycol 8,000 (50 w/v)
    50 μl of HEPES pH 7 (1 M)
    100 μl of Calcium chloride (1 M)
    50 μl of HEPES pH 8 (1 M)
    700 μl of water (100 v/v)
    D11
    • Nickel (II) chloride 0.05 M -
    • Sodium acetate 0.1 M pH 4
    • Polyethylene glycol monomethyl ether 2,000 12 w/v -
    pH 4
    50 μl of Nickel (II) chloride (1 M)
    0 μl of Sodium acetate pH 5.5 (1 M)
    240 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    100 μl of Sodium acetate pH 4 (1 M)
    610 μl of water (100 v/v)
    D12
    • Lithium nitrate 0.05 M -
    • ADA 0.1 M pH 6.2
    • Polyethylene glycol monomethyl ether 550 26 v/v -
    pH 6.2
    57 μl of ADA pH 6.5 (1 M)
    6 μl of Lithium nitrate (8 M)
    260 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    42 μl of ADA pH 5.5 (1 M)
    633 μl of water (100 v/v)
    E1
    • Magnesium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.7
    • Polyethylene glycol monomethyl ether 550 36 v/v -
    pH 8.7
    100 μl of Magnesium chloride (1 M)
    10 μl of tris chloride BCC pH 7 (1 M)
    360 μl of Polyethylene glycol monomethyl ether 550 (100 v/v)
    89 μl of tris chloride BCC pH 9 (1 M)
    440 μl of water (100 v/v)
    E2
    • Potassium chloride 0.1 M -
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 400 36 v/v -
    pH 6.5
    100 μl of Potassium chloride (1 M)
    100 μl of MES pH 6.5 (1 M)
    0 μl of MES pH 5.5 (1 M)
    360 μl of Polyethylene glycol 400 (100 v/v)
    440 μl of water (100 v/v)
    E3
    • Sodium chloride 0.15 M -
    • tri-Sodium citrate 0.05 M pH 3.7
    • Polyethylene glycol monomethyl ether 2,000 19 w/v -
    • 2-methyl-2,4-pentanediol BCC 6 v/v -
    pH 3.7
    120 μl of 2-methyl-2,4-pentanediol BCC (50 v/v)
    40 μl of tri-Sodium citrate pH 6.5 (1 M)
    9 μl of tri-Sodium citrate pH 3.1 (1 M)
    150 μl of Sodium chloride (1 M)
    380 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    300 μl of water (100 v/v)
    E4
    • Ammonium sulfate 0.125 M -
    • ADA 0.05 M pH 6.5
    • Polyethylene glycol 4,000 15 w/v -
    pH 6.5
    50 μl of ADA pH 6.5 (1 M)
    125 μl of Ammonium sulfate (1 M)
    300 μl of Polyethylene glycol 4,000 (50 w/v)
    525 μl of water (100 v/v)
    E5
    • Magnesium nitrate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 1,500 16 w/v -
    pH 5.6
    320 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    100 μl of Magnesium nitrate (1 M)
    480 μl of water (100 v/v)
    E6
    • Cadmium chloride 0.002 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 28 v/v -
    pH 7
    100 μl of HEPES pH 7 (1 M)
    280 μl of Polyethylene glycol 400 (100 v/v)
    20 μl of Cadmium chloride (0.1 M)
    600 μl of water (100 v/v)
    E7
    • Ammonium sulfate 0.34 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol 4,000 10 w/v -
    pH 5.6
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    340 μl of Ammonium sulfate (1 M)
    360 μl of water (100 v/v)
    E8
    • Calcium acetate 0.1 M -
    • Sodium chloride 0.075 M -
    • MOPS 0.1 M pH 7
    • Polyethylene glycol 400 24 v/v -
    pH 7
    55 μl of MOPS pH 6.5 (1 M)
    44 μl of MOPS pH 7.5 (1 M)
    75 μl of Sodium chloride (1 M)
    240 μl of Polyethylene glycol 400 (100 v/v)
    100 μl of Calcium acetate (1 M)
    485 μl of water (100 v/v)
    E9
    • Magnesium chloride 0.25 M -
    • MOPS 0.05 M pH 6.5
    • Polyethylene glycol 2,000 23 w/v -
    pH 6.5
    250 μl of Magnesium chloride (1 M)
    50 μl of MOPS pH 6.5 (1 M)
    0 μl of MOPS pH 7.5 (1 M)
    460 μl of Polyethylene glycol 2,000 (50 w/v)
    240 μl of water (100 v/v)
    E10
    • Sodium chloride 0.04 M -
    • Calcium chloride 0.01 M -
    • Sodium acetate 0.035 M pH 5.3
    • Polyethylene glycol 400 20 v/v -
    pH 5.3
    11 μl of Sodium acetate pH 5 (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of Sodium chloride (1 M)
    10 μl of Calcium chloride (1 M)
    23 μl of Sodium acetate pH 5.5 (1 M)
    515 μl of water (100 v/v)
    E11
    • HEPES 0.075 M pH 7
    • Polyethylene glycol 400 20 v/v -
    • Polyethylene glycol 4,000 5 w/v -
    • Polyethylene glycol 8,000 2.5 w/v -
    pH 7
    100 μl of Polyethylene glycol 4,000 (50 w/v)
    50 μl of Polyethylene glycol 8,000 (50 w/v)
    400 μl of Polyethylene glycol 400 (50 v/v)
    75 μl of HEPES pH 7 (1 M)
    375 μl of water (100 v/v)
    E12
    • Magnesium nitrate 0.05 M -
    • tri-Sodium citrate 0.1 M pH 5.6
    • Polyethylene glycol monomethyl ether 550 20 v/v -
    pH 5.6
    400 μl of Polyethylene glycol monomethyl ether 550 (50 v/v)
    100 μl of tri-Sodium citrate pH 5.6 (1 M)
    50 μl of Magnesium nitrate (1 M)
    450 μl of water (100 v/v)
    F1
    • Lithium sulfate 0.1 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 3,350 31 w/v -
    pH 7
    100 μl of tris chloride BCC pH 7 (1 M)
    620 μl of Polyethylene glycol 3,350 (50 w/v)
    100 μl of Lithium sulfate (1 M)
    180 μl of water (100 v/v)
    F2
    • Magnesium acetate 0.4 M -
    • tris chloride BCC 0.08 M pH 8.8
    • Polyethylene glycol 4,000 12 w/v -
    pH 8.8
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    5 μl of tris chloride BCC pH 7 (1 M)
    74 μl of tris chloride BCC pH 9 (1 M)
    400 μl of Magnesium acetate (1 M)
    280 μl of water (100 v/v)
    F3
    • Lithium nitrate 0.05 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 600 36 v/v -
    pH 6
    47 μl of MES pH 6.5 (1 M)
    6 μl of Lithium nitrate (8 M)
    53 μl of MES pH 5.5 (1 M)
    720 μl of Polyethylene glycol 600 (50 v/v)
    173 μl of water (100 v/v)
    F4
    • Lithium sulfate 0.1 M -
    • tri-Sodium citrate 0.1 M pH 5
    • Polyethylene glycol 400 26 v/v -
    pH 5
    100 μl of tri-Sodium citrate pH 5 (1 M)
    520 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of Lithium sulfate (1 M)
    280 μl of water (100 v/v)
    F5
    • Calcium chloride 0.125 M -
    • tris chloride BCC 0.02 M pH 7.4
    • Polyethylene glycol 3,000 16 w/v -
    pH 7.4
    17 μl of tris chloride BCC pH 7 (1 M)
    2 μl of tris chloride BCC pH 9 (1 M)
    320 μl of Polyethylene glycol 3,000 (50 w/v)
    125 μl of Calcium chloride (1 M)
    535 μl of water (100 v/v)
    F6
    • Calcium chloride 0.2 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 40 v/v -
    pH 8
    54 μl of tris chloride BCC pH 7 (1 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    200 μl of Calcium chloride (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    300 μl of water (100 v/v)
    F7
    • Sodium chloride 0.04 M -
    • Calcium chloride 0.125 M -
    • MES 0.035 M pH 5.5
    • Polyethylene glycol 400 20 v/v -
    pH 5.5
    35 μl of MES pH 5.5 (1 M)
    400 μl of Polyethylene glycol 400 (50 v/v)
    40 μl of Sodium chloride (1 M)
    125 μl of Calcium chloride (1 M)
    400 μl of water (100 v/v)
    F8
    • Magnesium chloride 0.02 M -
    • ADA 0.06 M pH 7
    • Polyethylene glycol 1,500 12 w/v -
    pH 7
    20 μl of Magnesium chloride (1 M)
    240 μl of Polyethylene glycol 1,500 (50 w/v)
    60 μl of ADA pH 7 (1 M)
    680 μl of water (100 v/v)
    F9
    • Nickel (II) chloride 0.01 M -
    • MES 0.05 M pH 5.5
    • Polyethylene glycol 400 42 v/v -
    pH 5.5
    50 μl of MES pH 5.5 (1 M)
    10 μl of Nickel (II) chloride (1 M)
    420 μl of Polyethylene glycol 400 (100 v/v)
    520 μl of water (100 v/v)
    F10
    • Potassium chloride 0.055 M -
    • MOPS 0.05 M pH 7
    • Polyethylene glycol monomethyl ether 2,000 11 w/v -
    pH 7
    55 μl of Potassium chloride (1 M)
    50 μl of MOPS pH 7 (1 M)
    220 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    675 μl of water (100 v/v)
    F11
    • Magnesium chloride 0.075 M -
    • HEPES 0.1 M pH 8
    • Polyethylene glycol 1,500 22 w/v -
    pH 8
    75 μl of Magnesium chloride (1 M)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    100 μl of HEPES pH 8 (1 M)
    385 μl of water (100 v/v)
    F12
    • Lithium chloride 0.1 M -
    • Glycine 0.05 M pH 9.5
    • Polyethylene glycol 400 40 v/v -
    pH 9.5
    50 μl of Glycine pH 9.5 (1 M)
    100 μl of Lithium chloride (1 M)
    400 μl of Polyethylene glycol 400 (100 v/v)
    450 μl of water (100 v/v)
    G1
    • Sodium acetate 0.325 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 400 20 v/v -
    pH 8
    54 μl of tris chloride BCC pH 7 (1 M)
    162 μl of Sodium acetate (2 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    200 μl of Polyethylene glycol 400 (100 v/v)
    537 μl of water (100 v/v)
    G2
    • Ammonium sulfate 0.25 M -
    • MES 0.1 M pH 5.5
    • Pentaerythritol ethoxylate (15/4 EO/OH) 35 v/v -
    pH 5.5
    350 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (100 v/v)
    100 μl of MES pH 5.5 (1 M)
    250 μl of Ammonium sulfate (1 M)
    300 μl of water (100 v/v)
    G3
    • tri-Potassium citrate 0.1 M pH 4
    • Polyethylene glycol 400 17 v/v -
    pH 4
    100 μl of tri-Potassium citrate pH 4 (1 M)
    340 μl of Polyethylene glycol 400 (50 v/v)
    560 μl of water (100 v/v)
    G4
    • Sodium chloride 0.07 M -
    • tri-Sodium citrate 0.075 M pH 3.7
    • Polyethylene glycol 400 23 v/v -
    pH 3.7
    460 μl of Polyethylene glycol 400 (50 v/v)
    70 μl of Sodium chloride (1 M)
    14 μl of tri-Sodium citrate pH 3.5 (1 M)
    60 μl of tri-Sodium citrate pH 4 (1 M)
    395 μl of water (100 v/v)
    G5
    • Barium chloride 0.15 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 26 v/v -
    pH 7
    150 μl of Barium chloride (1 M)
    520 μl of Polyethylene glycol 400 (50 v/v)
    100 μl of HEPES pH 7 (1 M)
    230 μl of water (100 v/v)
    G6
    • Ammonium formate 1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 25 v/v -
    pH 6
    47 μl of MES pH 6.5 (1 M)
    53 μl of MES pH 5.5 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    200 μl of Ammonium formate (5 M)
    450 μl of water (100 v/v)
    G7
    • Calcium chloride 0.06 M -
    • Magnesium chloride 0.06 M -
    • CHES 0.075 M pH 9
    • Polyethylene glycol 4,000 22 w/v -
    pH 9
    60 μl of Magnesium chloride (1 M)
    75 μl of CHES pH 9 (1 M)
    440 μl of Polyethylene glycol 4,000 (50 w/v)
    60 μl of Calcium chloride (1 M)
    365 μl of water (100 v/v)
    G8
    • Magnesium chloride 0.1 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 300 28 v/v -
    pH 6
    100 μl of Magnesium chloride (1 M)
    47 μl of MES pH 6.5 (1 M)
    53 μl of MES pH 5.5 (1 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    520 μl of water (100 v/v)
    G9
    • Ammonium thiocyanate 0.4 M -
    • tri-Sodium citrate 0.1 M pH 4.5
    • Polyethylene glycol 4,000 10 w/v -
    pH 4.5
    200 μl of Polyethylene glycol 4,000 (50 w/v)
    400 μl of Ammonium thiocyanate (1 M)
    100 μl of tri-Sodium citrate pH 4.5 (1 M)
    300 μl of water (100 v/v)
    G10
    • Sodium acetate 0.18 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol 400 27 v/v -
    • Pentaerythritol ethoxylate (15/4 EO/OH) 12 v/v -
    pH 6
    240 μl of Pentaerythritol ethoxylate (15/4 EO/OH) (50 v/v)
    47 μl of MES pH 6.5 (1 M)
    90 μl of Sodium acetate (2 M)
    53 μl of MES pH 5.5 (1 M)
    270 μl of Polyethylene glycol 400 (100 v/v)
    300 μl of water (100 v/v)
    G11
    • Sodium chloride 0.2 M -
    • CHES 0.1 M pH 9.5
    • Polyethylene glycol 8,000 10 w/v -
    pH 9.5
    200 μl of Polyethylene glycol 8,000 (50 w/v)
    100 μl of Sodium chloride (2 M)
    100 μl of CHES pH 9.5 (1 M)
    600 μl of water (100 v/v)
    G12
    • Magnesium acetate 0.01 M -
    • Sodium acetate 0.05 M pH 5
    • Polyethylene glycol 400 23 v/v -
    pH 5
    50 μl of Sodium acetate pH 5 (1 M)
    10 μl of Magnesium acetate (1 M)
    460 μl of Polyethylene glycol 400 (50 v/v)
    480 μl of water (100 v/v)
    H1
    • Barium chloride 0.1 M -
    • tris chloride BCC 0.1 M pH 8.5
    • Polyethylene glycol 300 28 v/v -
    pH 8.5
    100 μl of Barium chloride (1 M)
    21 μl of tris chloride BCC pH 7 (1 M)
    78 μl of tris chloride BCC pH 9 (1 M)
    280 μl of Polyethylene glycol 300 (100 v/v)
    520 μl of water (100 v/v)
    H2
    • tri-Sodium citrate 0.05 M pH 5
    • Polyethylene glycol monomethyl ether 2,000 25 w/v -
    pH 5
    50 μl of tri-Sodium citrate pH 5 (1 M)
    500 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    450 μl of water (100 v/v)
    H3
    • Sodium chloride 0.275 M -
    • HEPES 0.1 M pH 7
    • Polyethylene glycol 400 25 v/v -
    pH 7
    275 μl of Sodium chloride (1 M)
    100 μl of HEPES pH 7 (1 M)
    250 μl of Polyethylene glycol 400 (100 v/v)
    375 μl of water (100 v/v)
    H4
    • Sodium sulfate 0.15 M -
    • tris chloride BCC 0.1 M pH 7
    • Polyethylene glycol 400 27 v/v -
    pH 7
    150 μl of Sodium sulfate (1 M)
    100 μl of tris chloride BCC pH 7 (1 M)
    270 μl of Polyethylene glycol 400 (100 v/v)
    480 μl of water (100 v/v)
    H5
    • MES 0.1 M pH 6.5
    • Polyethylene glycol 1,500 22 w/v -
    pH 6.5
    100 μl of MES pH 6.5 (1 M)
    440 μl of Polyethylene glycol 1,500 (50 w/v)
    460 μl of water (100 v/v)
    H6
    • Cadmium chloride 0.001 M -
    • Glycine 0.1 M pH 9.5
    • 2-methyl-2,4-pentanediol BCC 60 v/v -
    pH 9.5
    100 μl of Glycine pH 9.5 (1 M)
    600 μl of 2-methyl-2,4-pentanediol BCC (100 v/v)
    10 μl of Cadmium chloride (0.1 M)
    290 μl of water (100 v/v)
    H7
    • tris chloride BCC 0.05 M pH 8.5
    • Polyethylene glycol 400 27 v/v -
    pH 8.5
    540 μl of Polyethylene glycol 400 (50 v/v)
    50 μl of tris chloride BCC pH 8.5 (1 M)
    410 μl of water (100 v/v)
    H8
    • Potassium acetate 0.25 M -
    • tris chloride BCC 0.1 M pH 8
    • Polyethylene glycol 300 22 v/v -
    pH 8
    50 μl of Potassium acetate (5 M)
    54 μl of tris chloride BCC pH 7 (1 M)
    45 μl of tris chloride BCC pH 9 (1 M)
    220 μl of Polyethylene glycol 300 (100 v/v)
    630 μl of water (100 v/v)
    H9
    • Magnesium chloride 0.125 M -
    • MES 0.1 M pH 6
    • Polyethylene glycol monomethyl ether 2,000 14 w/v -
    pH 6
    125 μl of Magnesium chloride (1 M)
    47 μl of MES pH 6.5 (1 M)
    53 μl of MES pH 5.5 (1 M)
    280 μl of Polyethylene glycol monomethyl ether 2,000 (50 w/v)
    495 μl of water (100 v/v)
    H10
    • Sodium chloride 0.4 M -
    • tris chloride BCC 0.05 M pH 8
    • Polyethylene glycol 600 26 v/v -
    pH 8
    27 μl of tris chloride BCC pH 7 (1 M)
    22 μl of tris chloride BCC pH 9 (1 M)
    260 μl of Polyethylene glycol 600 (100 v/v)
    200 μl of Sodium chloride (2 M)
    490 μl of water (100 v/v)
    H11
    • Ammonium acetate 0.2 M -
    • Sodium chloride 0.1 M -
    • BIS-TRIS 0.1 M pH 6
    • Polyethylene glycol 4,000 12 w/v -
    pH 6
    240 μl of Polyethylene glycol 4,000 (50 w/v)
    200 μl of Ammonium acetate (1 M)
    100 μl of BIS-TRIS pH 6 (1 M)
    50 μl of Sodium chloride (2 M)
    410 μl of water (100 v/v)
    H12
    • tri-Sodium citrate 0.09 M pH 5.6
    • Polyethylene glycol 400 30 v/v -
    pH 5.6
    600 μl of Polyethylene glycol 400 (50 v/v)
    90 μl of tri-Sodium citrate pH 5.6 (1 M)
    310 μl of water (100 v/v)